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1.
J Agric Food Chem ; 68(3): 799-807, 2020 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-31893634

RESUMEN

Onion is the most widely cultivated vegetable around the world. In this study, the isolation, concentration, quantification, and bioactivity evaluation of the phenolics in onion peels were investigated. Thirty-four phenolics, including 17 flavonoids and 17 nonflavonoid phenolics, were purified and identified. Among them, there were 2 new unusual epoxyflavanones and a new phenolic constituent, as well as 13 unreported constituents from the genus Allium. The total flavonoids were concentrated, and finally obtained 90.25% of flavonoid content extract. Fifteen main flavonoids were quantified using an ultra-performance liquid chromatography-photodiode array (UPLC-PDA), and quercetin (36.94%) and quercetin 4'-O-ß-d-glucopyranoside (15.81%) were the richest contents of flavonoids. The antioxidant and anti-neuroinflammatory capacities were evaluated, and the bioactive results indicated that the flavonoids in onion exhibited pronounced activities. The study suggested that the flavonoids in onion peels could be used in functional food.


Asunto(s)
Antiinflamatorios/química , Antioxidantes/química , Flavonoides/química , Cebollas/química , Extractos Vegetales/química , Animales , Antiinflamatorios/farmacología , Antioxidantes/farmacología , Flavonoides/farmacología , Ratones , Microglía/efectos de los fármacos , Microglía/inmunología , Extractos Vegetales/farmacología
2.
J Photochem Photobiol B ; 203: 111748, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31918235

RESUMEN

Nanotechnology is an emerged field to develop the plant mediated metal based nanodrugs by green method. In this current study, the zinc oxide metal based nanoparticles were developed using (Clausena lansium (Lour.) Skeels) Peel aqueous extracts and zinc nitrate. The C.L extract zinc nanoparticleswere indicated by the sharp peak seen at 350 nm utilizing the Ultraviolet-Visible spectroscopy (UV-Vis). The high peaks indicate the presence of phytochemicals and its functional groups in ZnONPs were studied by the Fourier Transform Infrared Spectroscopy (FT-IR). The X-Ray Diffraction analysis (XRD) explores the pattern and structure of ZnONPs as spherical and base-centered monoclinic crystalline shapes. The C.L extract with Zn nanoparticles were spherical in nature and the size of the synthesized particles were about 28.42 nm respectively. The autophagy (Beclin-1, LC3-I, LC3-II and ATG4B) and apoptotic (Bax, Bcl-2 and Caspase-3) proteins were regulated by the treatment with ZnONPs in SH-SY5Y neuroblastoma cells. The DNA loss or damage was occurred in the ZnONPs treatment and it was performed using Comet assay. The ZnONPs treatment generates the ROS in the cells and decreased its stability and viability. Addition of NAC prevents ROS in the cultured SH-SY5Y cells and prevents the cells from the apoptosis. We concluded that the ZnONPs potentially kills the neuroblastoma cells by producing the intracellular ROS.


Asunto(s)
Antineoplásicos/farmacología , Autofagia/efectos de los fármacos , Nanopartículas del Metal/química , Óxido de Zinc/química , Antineoplásicos/química , Apoptosis/efectos de los fármacos , Beclina-1/metabolismo , Caspasa 3/metabolismo , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Clausena/química , Clausena/metabolismo , Daño del ADN/efectos de los fármacos , Tecnología Química Verde , Humanos , Nanopartículas del Metal/toxicidad , Neuroblastoma/metabolismo , Neuroblastoma/patología , Extractos Vegetales/química , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Especies Reactivas de Oxígeno/metabolismo
3.
J Photochem Photobiol B ; 203: 111763, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31931382

RESUMEN

Photodynamic therapy (PDT) is a clinical modality that allows the destruction of tumor cells and microorganisms by reactive oxygen species, formed by the combination of photosensitizer (PS), molecular oxygen and adequate wavelength light. This research, through a clean methodology that involves pressurized liquids extraction (PLE), obtained a highly antimicrobial extract of Tetragonia tetragonoides, which rich in chlorophylls as photosensitizers. The Chlorophylls-based extract (Cbe-PLE) presented pharmacological safety, through the maintenance of cellular viability. In addition, Cbe-PLE showed great efficacy against Staphylococcus aureus, with severe dose-dependent damage to the cell wall of the pathogen. The obtained product has a high potential for the development of photostimulated phytotherapic formulations for clinical applications in localized infections, as a complementary therapeutic alternative to antibiotics.


Asunto(s)
Aizoaceae/química , Extractos Vegetales/química , Aizoaceae/metabolismo , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Clorofila/química , Clorofila/farmacología , Luz , Ratones , Pruebas de Sensibilidad Microbiana , Nanoestructuras/química , Fármacos Fotosensibilizantes/química , Fármacos Fotosensibilizantes/farmacología , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Tallos de la Planta/química , Tallos de la Planta/metabolismo , Staphylococcus aureus/efectos de los fármacos
4.
Chem Pharm Bull (Tokyo) ; 68(1): 91-95, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31902904

RESUMEN

Magnolia Flower is a crude drug used for the treatment of headaches, toothaches, and nasal congestion. Here, we focused on Magnolia kobus, one of the botanical origins of Magnolia Flower, and collected the flower parts at different growth stages to compare chemical compositions and investigate potential inhibitory activities against interleukin-2 (IL-2) production in murine splenic T cells. After determining the structures, we examined the inhibitory effects of the constituents of the bud, the medicinal part of the crude drug, against IL-2 production. We first extracted the flower parts of M. kobus from the bud to fallen bloom stages and analysed the chemical compositions to identify the constituents characteristic to the buds. We found that the inhibitory activity of the buds against IL-2 production was more potent than that of the blooms. We isolated two known compounds, tiliroside (1) and syringin (2), characteristic to the buds from the methanol (MeOH) extract of Magnolia Flower. Moreover, we examined the inhibitory activities of both compounds against IL-2 production and found that tiliroside (1) but not syringin (2), showed strong inhibitory activity against IL-2 production and inhibited its mRNA expression. Thus, our strategy to examine the relationship between chemical compositions and biological activities during plant maturation could not only contribute to the scientific evaluation of medicinal parts of crude drugs but also assist in identifying biologically active constituents that have not yet been reported.


Asunto(s)
Interleucina-2/metabolismo , Magnolia/química , Extractos Vegetales/química , Animales , Línea Celular , Flavonoides/química , Flavonoides/aislamiento & purificación , Flavonoides/farmacología , Flores/química , Flores/metabolismo , Glucósidos/química , Glucósidos/aislamiento & purificación , Glucósidos/farmacología , Interleucina-2/genética , Magnolia/metabolismo , Ratones , Fenilpropionatos/química , Fenilpropionatos/aislamiento & purificación , Fenilpropionatos/farmacología , Linfocitos T/citología , Linfocitos T/efectos de los fármacos , Linfocitos T/metabolismo
5.
J Photochem Photobiol B ; 202: 111674, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31778953

RESUMEN

The present work showed the preparation of gold (Au NPs) and silver nanoparticles (Ag NPs) using aqueous leaf extract of Clinacanthus nutans Lindau (C. Lindau). The prepared NPs were studied using various microscopic and spectroscopic techniques, which confirmed the formation of crystalline Ag NPs, Au NPs that are stabilized with C. Lindau extract polyphenols. The prepared Au NPs and Ag NPs are studied to assess their comparative analgesic and muscle relaxant activities conducted on BALB/c mice. The muscle relaxant studies displayed that Ag NPs were comparatively higher efficient than Au NPs and methanolic C. Lindau extract in traction examination. Additionally, the analgesic studies exhibited that Ag NPs, Au NPs showed maximum percentage reduction in acetic acid induced writhing at the concentrations of 50, 100 and 150 mg/kg body weight. Further, these results conclude that as prepared Au NPs and Ag NPs prepared from C. Lindau leaf extract demonstrated very good analgesic and muscle relaxant activities for the use of pain management in nursing care.


Asunto(s)
Acanthaceae/química , Analgésicos/síntesis química , Oro/química , Nanopartículas del Metal/química , Plata/química , Acanthaceae/metabolismo , Ácido Acético/toxicidad , Analgésicos/farmacología , Analgésicos/uso terapéutico , Animales , Tecnología Química Verde , Nanopartículas del Metal/uso terapéutico , Ratones , Ratones Endogámicos BALB C , Relajación Muscular/efectos de los fármacos , Atención de Enfermería , Dolor/inducido químicamente , Dolor/tratamiento farmacológico , Manejo del Dolor/métodos , Extractos Vegetales/química , Hojas de la Planta/química , Hojas de la Planta/metabolismo
6.
J Agric Food Chem ; 68(1): 117-127, 2020 Jan 08.
Artículo en Inglés | MEDLINE | ID: mdl-31820963

RESUMEN

Six new quassinoids, named kumulactone F (1), kumulactone G (2), kumulactone H (4), kumulactone I (5), kumulactone J (6), and kumulactone K (7), a pair of undescribed epimers α- and ß-nigakihemiacetal G (3), 15 known quassinoids (8-22), and a mixture of the known compounds α- and ß-neoquassin (23) were separated from the dried stems of the medical plants Picrasma quassioides. The chemical structures of all of the new compounds were established by spectroscopic data analyses (HR-ESI-MS, 1D and 2D NMR spectroscopy, and electronic circular dichroism (ECD)). Biologically, compounds 9 and 21 showed toxicity toward the Asian citrus psyllid Diaphorina citri Kuwayama with potent activity even equal to that of the positive control (Abamectin), compound 11 exhibited an excellent neuroprotective effect against SH-SY5Y cells which were pretreated by H2O2 with potent activity equal to that of the positive control (Trolox), and none of them showed cytotoxic activity toward the HeLa or A549 cell lines (IC50 > 100 µM).


Asunto(s)
Hemípteros/efectos de los fármacos , Insecticidas/farmacología , Fármacos Neuroprotectores/farmacología , Picrasma/química , Extractos Vegetales/farmacología , Cuassinas/farmacología , Animales , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Hemípteros/crecimiento & desarrollo , Humanos , Peróxido de Hidrógeno/toxicidad , Insecticidas/química , Neuronas/citología , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/química , Extractos Vegetales/química , Cuassinas/química
7.
J Agric Food Chem ; 68(3): 884-891, 2020 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-31825610

RESUMEN

Having a long history of traditional medicinal applications, Papaver somniferum is also known as a source of various pharmacologically highly active opiates. Consequently, their detection from plant extracts is an important analytical task and generally addressed by methods of GC-MS and LC-MS. However, opiates do also show structural similarities to matrix molecules used in matrix-assisted laser desorption ionization (LDI) and may therefore ionize upon simple laser irradiation. Following this analytical approach, the present work thoroughly evaluated the direct detection of opiates by matrix-free LDI in crude extracts of P. somniferum. The method facilitated the identification of 10 reported opiates by their molecular formulas without any chromatographic prepurification. Moreover, a principal component analysis based on LDI-MS data permitted the correct grouping of all extracts according to their inherent chemistry. Concluding experiments on serial dilutions of thebaine further evaluated potential quantitative applications of the method. Overall results highlight the promising potential of LDI-MS for the swift detection of opiates in complex mixtures.


Asunto(s)
Alcaloides Opiáceos/química , Papaver/química , Extractos Vegetales/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Rayos Láser , Estructura Molecular , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/instrumentación
8.
J Agric Food Chem ; 68(3): 769-778, 2020 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-31826608

RESUMEN

Previous work with Caco-2 cell cultures has shown that individual polyphenols can either promote or inhibit iron uptake. This investigation was designed to characterize the relationship between iron bioavailability and seed coat polyphenol composition in a panel of 14 yellow beans representing five market classes with the potential for fast cooking time and high iron content. The study included two white and two red mottled bean lines, which represent high and low iron bioavailability capacity in dry beans, respectively. Polyphenols were measured quantitatively by high-performance liquid chromatography-mass spectrometry (HPLC-MS)/UV and iron bioavailability of seed coat extracts was measured in Caco-2 assays. Thirteen of the yellow bean seed types contained high concentrations (up to 35.3 ± 2.7 µmol/g) of kaempferol 3-glucoside (k 3-g), a known promoter of iron uptake. A general association between the ratio of promoting to inhibiting polyphenols (P/I) and iron uptake was observed. The presence of iron uptake inhibiting condensed tannins proportionately countered the promotional effects of kaempferol compounds. Unidentified factors present in seed coats other than polyphenols also appeared to affect iron uptake.


Asunto(s)
Hierro/metabolismo , Phaseolus/química , Extractos Vegetales/química , Polifenoles/química , Disponibilidad Biológica , Transporte Biológico , Células CACO-2 , Humanos , Hierro/química , Phaseolus/clasificación , Phaseolus/metabolismo , Extractos Vegetales/metabolismo , Polifenoles/metabolismo , Semillas/química , Semillas/metabolismo
9.
J Agric Food Chem ; 68(1): 88-96, 2020 Jan 08.
Artículo en Inglés | MEDLINE | ID: mdl-31826619

RESUMEN

Solanum rostratum is a worldwide malignant invasive weed, causing serious harm to the ecological environment and biodiversity. Strong chemical defense against herbivorous insects is supposed to be one of the successful invasive mechanisms of this exotic plant. However, the real defense components and their action mechanisms and distributions are still unknown. To address these problems, we bioassay-guided isolated compounds from the aerial part of S. rostratum and determined their structures using high-resolution electrospray ionization mass spectrometry, nuclear magnetic resonance, and electronic circular dichroism calculation. One new and seven known compounds were identified, and all of the isolates exhibited different levels of antifeedant activities, especially compounds 1 and 4. Consistently, compounds 1 and 4 displayed potent inhibitory effects on antifeedant-related enzymes (AchE and CarE). The action mechanisms of active compounds 1 and 4 were revealed by molecular docking and molecular dynamic simulation studies. Furthermore, the distributions of the active compounds in leaves, stems, and flowers were also analyzed by liquid chromatography-mass spectrometry.


Asunto(s)
Conducta Alimentaria/efectos de los fármacos , Insecticidas/farmacología , Mariposas Nocturnas/efectos de los fármacos , Extractos Vegetales/farmacología , Malezas/química , Solanum/química , Animales , Flores/química , Flores/metabolismo , Insecticidas/química , Insecticidas/aislamiento & purificación , Insecticidas/metabolismo , Simulación del Acoplamiento Molecular , Estructura Molecular , Mariposas Nocturnas/fisiología , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/metabolismo , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Malezas/metabolismo , Metabolismo Secundario , Solanum/metabolismo , Espectrometría de Masa por Ionización de Electrospray
10.
Fitoterapia ; 140: 104444, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31790768

RESUMEN

Alpinia zerumbet (Pers.) B.L.Burtt & R.M.Sm. (Zingiberaceae) is a perennial plant native to the East Indies and is widely distributed in South America, Oceania, and Asia. The mature fruits of the plant have been used in traditional medicine in China. In this study, we compared the chemical constituents in the methanol extracts of the leaves, the placenta, the pericarps, and the seeds obtained from the same plant using LC-MS, and we examined the NO inhibitory activities of the respective extracts and the isolated compounds. As a result of LC-MS analyses, kavalactone derivatives (1-6) were detected in the methanol extracts of the leaves, placenta, and pericarps. Of these, compound 6 was identified as a new asymmetrical cyclobutane dimer of 5,6-dehydrokawain. Quantitative analysis showed that the total amounts of kavalactone derivatives were highest in the methanol extract of the pericarps. Moreover, the results of measurements of the anti-inflammatory activity revealed that the pericarps extract showed the strongest activity. The compounds responsible for the anti-inflammatory activity of the extracts from A. zerumbet were identified. Of these, five were known kavalactone derivatives and one was a new kavalactone derivative (aniba dimer C). The results showed that the pericarps of A. zerumbet are a rich source of kavalactone derivatives, and that the pericarps of A. zerumbet can be utilized as an important medicinal resource.


Asunto(s)
Alpinia/química , Antiinflamatorios/farmacología , Lactonas/farmacología , Animales , Antiinflamatorios/aislamiento & purificación , Células Cultivadas , Hepatocitos/efectos de los fármacos , Japón , Lactonas/aislamiento & purificación , Estructura Molecular , Óxido Nítrico/metabolismo , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Extractos Vegetales/química , Hojas de la Planta/química , Ratas
11.
J Photochem Photobiol B ; 202: 111715, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31790882

RESUMEN

Biomimetic gold nanoparticles of biological origin have created a significant impact on the field of biomedicine due to the great expectations of its applications. Because of this, the influences of biomimetic gold nanoparticles have been immensely studied, targeting various cancer cells. However, the impact of biomimetic gold nanoparticles against normal non-cancerous cells is scanty, which impose several limitations in their utility. Taking this as a challenge, we in this study report the biomimetic gold nanoparticles from marine seaweed Gelidium pusillum (G. pusillum) to evaluate its cytotoxic and biocompatible ability evidenced by fluorescence-based assays in cultured cells. The gold nanoparticles obtained in the study were spherical shaped with a mean diameter of 12 ± 4.2 nm. The seaweed extract plays a crucial role in stabilizing the gold nanoparticles to avoid aggregation and coalescence. At an IC50 concentration of 43.09 ± 1.6 µgmL-1, the biomimetic gold nanoparticles were found to be toxic to cancerous cells (MDA-MB-231). Whereas, biomimetic gold nanoparticles exhibit significant biocompatibility with human embryonic kidney cells even at a higher concentration of 150 µgmL-1. The morphological based fluorescence assays confirmed the ability of biomimetic gold nanoparticles in inducing apoptosis and thereby kills cancer cells. Altogether, the gold nanoparticles were safe to normal cells and did not show a significant impact. Hence, the novel biomimetic gold nanoparticles hold potential as multifaceted agent and can further be taken up to various biomedical applications.


Asunto(s)
Materiales Biocompatibles/química , Oro/química , Nanopartículas del Metal/química , Apoptosis/efectos de los fármacos , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/farmacología , Línea Celular Tumoral , Tecnología Química Verde , Células HEK293 , Humanos , Nanopartículas del Metal/toxicidad , Tamaño de la Partícula , Extractos Vegetales/química , Algas Marinas/química , Algas Marinas/metabolismo
12.
J Photochem Photobiol B ; 202: 111718, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31790883

RESUMEN

Metallic nanoparticles were extensively examined to explore their impending exploitations over pharmaceutical purposes. Current work attempting to explores the cytotoxic capacity of zinc oxide (ZnO) nanoparticles besides to human melanoma cell line (A375). Viability of cells was resoluted, and the promising cytotoxicity potential was exhibited by zinc oxide nanoparticles. Cellular adhesion and morphology was determined by propidium iodide assay. Characterization studies like UV-Spectroscopy, X-ray diffraction (XRD) investigation, transmission electron microscope (TEM), energy dispersive X-ray (EDX) Spec, and Fourier transform infrared (FT-IR) examination confirms the accessibility of measurement, form and volume. The mRNA expression of apoptotic genes like caspase 3, 8 and 9 was elevated followed by the exposure to ZnO nanoparticles and it was narrowly proved that ZnO nanoparticles stimulates the apoptotic cell necrosis at the transcriptional stage. Cardiospermum halicacabum down regulated the apoptotic gene expressions. Reactive oxygen species (ROS) accumulation was augmented at concentration reliant mode, that changed normalize numerous indicator pathways and manipulate the kinetic cellular actions. ZnO nanoparticle synthesized Cardiospermum halicacabum might persuades programmed cell necrosis via elevated ROS levels in cells. CH-ZnONPs was further stimulates the markers of apoptosis and aggravates necrosis of cancerous cells, toxicity to cells, and accretion of ROS. With sourced on above whole data, this might accomplished that CH-ZnONPs amalgamated Cardiospermum halicacabum appreciably possessed a toxicity to human melanoma cells (A375) via provoking the apoptotic cell necrosis, entailed feasible efficacy of CH-ZnONPs besides malignancy management.


Asunto(s)
Antineoplásicos/síntesis química , Apoptosis , Nanopartículas del Metal/química , Sapindaceae/química , Antineoplásicos/química , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Adhesión Celular/efectos de los fármacos , Línea Celular Tumoral , Regulación hacia Abajo/efectos de los fármacos , Tecnología Química Verde , Humanos , Melanoma/metabolismo , Melanoma/patología , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Extractos Vegetales/química , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Sapindaceae/metabolismo , Óxido de Zinc/química
13.
J Photochem Photobiol B ; 202: 111644, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31770706

RESUMEN

Management of degenerative spine pathologies frequently leads to the need for bone growth. Rehmanniae Radix (RR), a Chinese herbal formulation was found to exhibit numerous therapeutic properties including its potent effect against cancer cell lines. However, the underlying mechanism through which the Zinc oxide nanoparticles (ZnONPs) synthesized from Rehmanniae Radix exerts its anti-cancer activity against osteosarcoma cell line MG-63 needs to be explored. Therefore, the study was performed to evaluate the anticancer, cytotoxicity and apoptotic effectiveness of ZnONPs from RR against MG-63 cells. Characterization studies such UV-vis spectroscopy, FTIR, TEM and XRD analysis were performed. Cytotoxicity assay, mitochondrial membrane potential (MMP), morphological examination of cells and formation of reactive oxygen species (ROS), and apoptosis inducing ability of RR were evaluated by various procedures. Western blot analysis of apoptotic markers such as Bax, caspase-3 and caspase-9 were also performed. RR was found to inhibit growth of MG-63 cells at increasing dose. AO/EB staining confirmed the apoptotic efficacy of ZnONPs induced by RR in MG-63 cells. ZnONPs was also found to initiate increased generation of ROS and decreased MMP. Decreased MMP has resulted in increased levels of apoptotic proteins Bax, caspase-3 and caspase-9 and induction of apoptosis was substantiated by western blot analysis. The outcomes of the work propose that ZnONPs from RR exhibits strong anticancer action and inducing apoptosis on MG-63 cells via stimulating increased generation of ROS. Thus, ZnONPs from RR might be used as a hopeful drug target against several types of cancer cell lines.


Asunto(s)
Caspasa 3/metabolismo , Tecnología Química Verde , Nanopartículas del Metal/química , Óxido de Zinc/química , Proteína X Asociada a bcl-2/metabolismo , Caspasa 3/genética , Adhesión Celular/efectos de los fármacos , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Osteosarcoma/metabolismo , Osteosarcoma/patología , Extractos Vegetales/química , Especies Reactivas de Oxígeno/metabolismo , Rehmannia/química , Rehmannia/metabolismo , Proteína X Asociada a bcl-2/genética
14.
J Photochem Photobiol B ; 202: 111706, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31775112

RESUMEN

The noble metal such as Ag and Au doped CeO2 nanoparticles was successfully prepared by ionic liquid assisted hydrothermal method in the presence of Justicia adhatoda leaves extract and were used as an antibacterial and anticancer agent. The FT-IR and Raman spectrum exhibit the peaks at 460 and 464 cm-1 assigned to CeO stretching vibrations of NPs. The electron microscopic micrographs confirmed the spherical shaped morphology of prepared NPs. The insertion of Ag and Au ions into the CeO2 surface creates lattice defects the leads to reduce the band gap energy of Ag-Au/CeO2 at 3.15 eV. The XRD results suggested the average crystalline size of the silver­gold loaded CeO2 was 28 nm. From the elemental mapping images, we have visualized that existence and uniform distribution of Ag, Au, Ce, and O in the prepared nanomaterials. The antibacterial activity of unloaded and bimetal loaded CeO2 NPs was evaluated with Gram-positive and Gram-negative bacteria using disc diffusion assay. The AgAu loaded CeO2 NPs exhibited the highest zone of inhibition against E. coli and S. aureus strains when compared with pristine CeO2, Ag loaded CeO2, and Au loaded CeO2 NPs. In addition, the 100 µg mL-1 of CeO2, Ag/CeO2, Au/CeO2 and Ag-Au/CeO2 NPs exposed the 50, 51, 52 and 56% of anticancer activity against the HeLa cells respectively. Overall, this study concludes that the ionic liquid functionalized green synthesized bimetal loaded cerium oxide NPs showed potent antibacterial and anticancer activities.


Asunto(s)
Antibacterianos/química , Antineoplásicos/química , Líquidos Iónicos/química , Justicia/química , Nanopartículas del Metal/química , Antibacterianos/síntesis química , Antibacterianos/farmacología , Antineoplásicos/síntesis química , Antineoplásicos/farmacología , Supervivencia Celular/efectos de los fármacos , Cerio/química , Pruebas Antimicrobianas de Difusión por Disco , Oro/química , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Tecnología Química Verde , Células HeLa , Humanos , Justicia/metabolismo , Nanopartículas del Metal/toxicidad , Tamaño de la Partícula , Extractos Vegetales/química , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Plata/química
15.
J Photochem Photobiol B ; 202: 111713, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31760373

RESUMEN

The field of nanobiotechnology and nanomedicine paves way for the use of several nanoparticles. Especially, in biomedical applications, the silver nanoparticles (AgNPs), gold nanoparticles (AuNPs) and palladium nanoparticles (PdNPs) are found most vital and promising, among other nanoparticles. The biomedical activities of these particles mostly depend on their shape, size and distribution. Preparation of these particles in an eco-friendly method is an immediate need of the society. Herein, AuNPs, AgNPs and PdNPs (MNPS) were synthesized by Solanum nigrum Leaves (SNL) extract. The structural and morphological studies were carried out by using TEM, XRD and EDAX, while the optical and chemical properties were studied using UV-visible spectrum and FTIR spectroscopy. The particles obtained were found to possess a FCC (Face Centered Cubic) structure. TEM images of Ag, Au and PdNPs showed spherical well dispersed nanoparticles with average size of 3.46 nm, 9.39 nm and 21.55 nm respectively. The FTIR spectra confirmed polyphenols and antioxidants in SNL extract act as reducing and capping agents respectively in the synthesis of MNPs. The EDX technique confirmed the presence of silver, gold and palladium nanoparticles. Antimicrobial studies noted that the AgNPs have effective inhibition against E. coli. The complete reduction of 4-Nitrophenol and the formation of 4-Aminophenol with the presence of NaBH4 was chosen for the study of catalytic activities of the prepared MNPs. The reduction time of Au and Pd catalyst were smaller compared to that of Ag. This viable preparation method for producing small spherical shaped nanoparticles expected to the applied to the fields of nanomedicine.


Asunto(s)
Antibacterianos/química , Oro/química , Nanopartículas del Metal/química , Paladio/química , Plata/química , Solanum nigrum/química , Antibacterianos/síntesis química , Antibacterianos/farmacología , Catálisis , Pruebas Antimicrobianas de Difusión por Disco , Escherichia coli/efectos de los fármacos , Tecnología Química Verde , Nanopartículas del Metal/toxicidad , Nitrofenoles/química , Tamaño de la Partícula , Extractos Vegetales/química , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Solanum nigrum/metabolismo
16.
J Photochem Photobiol B ; 202: 111652, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31760374

RESUMEN

The Biocompatibility and stability of nanoparticles using plants have been widely investigated due to its applications in the biomedical industry. Currently, there is a growing interest in nanoparticles in bone remodelling. Artemisia annua is an herbal plant commonly used in the treatment of various ailments. This study investigated the zinc oxide nanoparticles (ZnO NPs) using the green synthesis technique from A. annua and the effects of A. annua ZnO-NPs on osteoblast differentiation and inhibition of osteoclast formation. The formulated ZnO-NPs from A. annua were characterized by using various spectroscopic and microscopic methods Fourier transform-infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and UV-Visible spectroscopy. The disc diffusion method was adopted to test the antimicrobial efficacy of ZnO-NPs. The viability of MG-63 cells were assayed by MTT test and Osteogenic-related assays like Real-time PCR and Mineralization assay were adopted to determine the effects of A. annua ZnO-NPs on the multiplication and differentiation of human osteoblast-like MG-63 cells. The characterization of A. annua ZnO-NPs revealed the crystalline nature with high zinc content and the presence of bioactive compounds from A. annua extract. The synthesized A. annua ZnO-NPs indicate significant antimicrobial potential. Besides, A. annua ZnO-NPs enhanced the proliferation, differentiation, and mineralization without causing significant cytotoxic impact on MG-63 cells. These effects indicate that A. annua ZnO-NPs can both stimulate bone formation via the differentiation of MG-63 cells. Hence, it was concluded that A. annua ZnO-NPs can be a promising agent for the treatment of bone deformities and bone-related diseases, however further research also required to explore the clear mechanism of A. annua ZnO-NPs in the formation and differentiation of MG-63 cells.


Asunto(s)
Artemisia annua/química , Diferenciación Celular , Proliferación Celular , Nanopartículas del Metal/química , Antiinfecciosos/síntesis química , Antiinfecciosos/química , Antiinfecciosos/farmacología , Artemisia annua/metabolismo , Calcio/metabolismo , Candida/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Tecnología Química Verde , Humanos , Nanopartículas del Metal/toxicidad , Osteoblastos/citología , Osteoblastos/metabolismo , Osteogénesis/efectos de los fármacos , Corteza de la Planta/química , Corteza de la Planta/metabolismo , Extractos Vegetales/química , Óxido de Zinc/química
17.
J Photochem Photobiol B ; 202: 111705, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31812087

RESUMEN

The procurance of gold nanoparticles in the plant extracts is an excellent way to attain nanomaterials natural and eco-friendly nanomaterials. The Dehydrated roots of Chinese Euphorbia fischeriana flowering plant are called "Lang-Du". In this study, the retrieving of gold nanoparticles from Euphorbia fischeriana root was amalgamated by standard procedure. Fabricated gold nanoparticles were portrayed through the investigations of ultraviolet and visible spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FTIR), High resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD). The UV-Vis and FTIR results explicated the obtained particles were sphere-shaped and the terpenoids of Euphorbia fischeriana had strong communications with gold surface. The HRTEM and XRD images exposed the produced gold nanoparticles had an extreme composition of crystal arrangement and excellent uniformed size of particles. In our study, the Isoprenaline induced myocardial damage established the elevation in TBARS, LOOH of heart tissues and notable decline in antioxidant enzymes SOD, CAT, GPx, and GSH. This biochemical result was additionally proved by histopathological assessment. Remarkably, the pretreatment with EF-AuNps(50 mg/kg b.w) illustrated stabilized levels of serum creatine and cardiotropins in myocardial infarcted animals. And further we understood the essential function of NF-ƙB, TNF-α, IL-6 signaling molecules and its way progression in the development of vascular tenderness.


Asunto(s)
Euphorbia/química , Oro/química , Nanopartículas del Metal/uso terapéutico , Infarto del Miocardio/prevención & control , Animales , Antioxidantes/química , Catalasa/genética , Catalasa/metabolismo , Creatina Quinasa/sangre , Citocinas/metabolismo , Euphorbia/metabolismo , Tecnología Química Verde , Isoproterenol/toxicidad , Nanopartículas del Metal/química , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/metabolismo , Miocardio/metabolismo , Miocardio/patología , Extractos Vegetales/química , Raíces de Plantas/química , Ratas , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo
18.
Food Chem Toxicol ; 135: 110949, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31751643

RESUMEN

In 2015, the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA) initiated a program for the re-evaluation of the safety of over 250 natural flavor complexes (NFCs) used as flavor ingredients. This publication, third in the series, considers NFCs composed primarily of constituents with the 3-phenyl-2-propenyl or a cinnamyl functional group, using the procedure outlined in 2005 and updated in 2018 to evaluate the safety of naturally-occurring mixtures for their intended use as flavor ingredients. The procedure relies on a complete chemical characterization of the NFC intended for commerce and organization of each NFC's chemical constituents into well-defined congeneric groups. The safety of the NFC is evaluated using the well-established and conservative threshold of toxicological concern (TTC) concept in addition to data on absorption, metabolism and toxicology of members of the congeneric groups and the NFC under evaluation. Six NFCs from the Myroxylon and Cinnamomum genera, Balsam Oil, Peru (FEMA 2117), Tolu Balsam Extract (FEMA 3069), Cassia Bark Extract (FEMA 2257), Cassia Bark Oil (FEMA 2258), Cinnamon Bark Extract (FEMA 2290) and Cinnamon Bark Oil (FEMA 2291) were evaluated and affirmed as generally recognized as safe (GRAS) under their conditions of intended use as flavor ingredients.


Asunto(s)
Cinnamomum/química , Aromatizantes/toxicidad , Myroxylon/química , Aceites Volátiles/toxicidad , Extractos Vegetales/toxicidad , Animales , Línea Celular , Seguridad de Productos para el Consumidor , Aromatizantes/química , Humanos , Nivel sin Efectos Adversos Observados , Aceites Volátiles/química , Extractos Vegetales/química , Medición de Riesgo
19.
J Agric Food Chem ; 68(2): 485-502, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31805232

RESUMEN

In this study we report a detailed investigation of the polyphenol composition of Protea pure (P. cynaroides and P. neriifolia) and hybrid cultivars (Black beauty and Limelight). Aqueous methanol extracts of leaf and bract tissues were analyzed by ultrahigh pressure liquid chromatography hyphenated to photodiode array and ion mobility-high resolution mass spectrometric (UHPLC-PDA-IM-HR-MS) detection. A total of 67 metabolites were characterized based on their relative reversed phase (RP) retention, UV-vis spectra, low and high collision energy HR-MS data, and collisional cross section (CCS) values. These metabolites included 41 phenolic acid esters and 25 flavonoid derivatives, including 5 anthocyanins. In addition, an undescribed hydroxycinnamic acid-polygalatol ester, caffeoyl-O-polygalatol (1,5-anhydro-[6-O-caffeoyl]-sorbitol(glucitol)) was isolated and characterized by 1D and 2D NMR for the first time. This compound and its isomer are shown to be potential chemo-taxonomic markers.


Asunto(s)
Fenoles/química , Extractos Vegetales/química , Proteaceae/química , Cromatografía Líquida de Alta Presión , Espectrometría de Masas , Estructura Molecular , Hojas de la Planta/química , Proteaceae/genética
20.
J Photochem Photobiol B ; 202: 111700, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31810039

RESUMEN

Zinc oxide (ZnO), an inorganic metal oxide established in the form of nanoparticles, has considerable biological properties. The current research uses Selaginella convolute (S. convolute) leaf extract to establish ZnO NPs and to assess their use in pain management. S. Convolute leaf extract mediated ZnO NPs were characterized by modern techniques and instruments such as Fourier transforms infrared spectroscopy (FTIR), electron microscopy, X-ray diffraction (XRD), and Ultraviolet-vis-spectroscopy (UV-vis), energy dispersive X-ray spectroscopy (EDX), indicating the emergence of spherical NPs of which is around 40 nm. The FTIR spectrum also signified that S. convolute plant extract polyphenols acted as a capping ligand for the fabricated ZnO NPs. Possessed ZnO NPs have shown important characteristics of muscle relaxing and antinociceptive. A concentration dependent acetic acid induced writhing effect was noted for both S. convolute extract and ZnO NPs. S. convolute plant extract and ZnO NPs are found to exhibit highest muscle relaxation effect in both traction and chimney tests and no sedative effect was shown by both ZnO NPs and plant extract. The present results showed that the S. convolute leaves extract is a very effective green reducing agent for the preparation of ZnO NPs and the prepared NPs can be used in pain management in emerging nursing care in future.


Asunto(s)
Nanopartículas del Metal/química , Selaginellaceae/química , Óxido de Zinc/química , Ácido Acético/toxicidad , Animales , Tecnología Química Verde , Locomoción/efectos de los fármacos , Nanopartículas del Metal/uso terapéutico , Nanopartículas del Metal/toxicidad , Ratones , Ratones Endogámicos BALB C , Relajación Muscular/efectos de los fármacos , Dolor/inducido químicamente , Dolor/tratamiento farmacológico , Manejo del Dolor , Extractos Vegetales/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Selaginellaceae/metabolismo
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