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1.
Int J Immunopathol Pharmacol ; 35: 20587384211005645, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33779346

RESUMEN

Protective effects of peroxiredoxin 6 (PRDX6) in RIN-m5F ß-cells and of thymulin in mice with alloxan-induced diabetes were recently reported. The present work was aimed at studying the efficiency of thymulin and PRDX6 in a type 1 diabetes mellitus model induced by streptozotocin in mice. Effects of prolonged treatment with PRDX6 or thymic peptide thymulin on diabetes development were evaluated. We assessed the effects of the drugs on the physiological status of diabetic mice by measuring blood glucose, body weight, and cell counts in several organs, as well as effects of thymulin and PRDX6 on the immune status of diabetic mice measuring concentrations of pro-inflammatory cytokines in blood plasma (TNF-α, interleukin-5 and 17, and interferon-γ), activity of NF-κB and JNK pathways, and Hsp90α expression in immune cells. Both thymulin and PRDX6 reduced the physiological impairments in diabetic mice at various levels. Thymulin and PRDX6 provide beneficial effects in the model of diabetes via very different mechanisms. Taken together, the results of our study indicated that the thymic peptide and the antioxidant enzyme have anti-inflammatory functions. As increasing evidences show diabetes mellitus as a distinct comorbidity leading to acute respiratory distress syndrome and increased mortality in patients with COVID-19 having cytokine storm, thymulin, and PRDX6 might serve as a supporting anti-inflammatory treatment in the therapy of COVID 19 in diabetic patients.


Asunto(s)
MAP Quinasa Quinasa 4/metabolismo , FN-kappa B/metabolismo , Peroxiredoxina VI , Transducción de Señal , Factor Tímico Circulante , Animales , Antiinflamatorios/metabolismo , Antiinflamatorios/farmacología , Antioxidantes/metabolismo , Antioxidantes/farmacología , /inmunología , Diabetes Mellitus/tratamiento farmacológico , Diabetes Mellitus/inmunología , Diabetes Mellitus Experimental/inmunología , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/terapia , Descubrimiento de Drogas , Interferón gamma/sangre , Interleucinas/sangre , Ratones , Peroxiredoxina VI/metabolismo , Peroxiredoxina VI/farmacología , Transducción de Señal/efectos de los fármacos , Transducción de Señal/inmunología , Factor Tímico Circulante/metabolismo , Factor Tímico Circulante/farmacología , Factor de Necrosis Tumoral alfa/sangre
2.
BMC Infect Dis ; 21(1): 250, 2021 Mar 10.
Artículo en Inglés | MEDLINE | ID: mdl-33691633

RESUMEN

BACKGROUND: Infants with bronchiolitis have an increased risk of developing recurrent wheezing and asthma. However, the risk factors for the development of recurrent wheezing after bronchiolitis remains controversial. Our study was to investigate risk factors of post-bronchiolitis recurrent wheezing. METHODS: Infants with bronchiolitis were enrolled from November 2016 through March 2017. Nasopharyngeal aspirates were obtained for detection of respiratory viruses which were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and direct immunofluorescent assay. Serum cytokines including TSLP, IL2, IL13, TIMP-1, MMP-9, IL33, IL5, IL4, IL25, TNF- α and MIP-1α were measured by flow cytometry. Patients were followed up every 3 months for a duration of 2 years by telephone or at outpatient appointments. RESULTS: We enrolled 89 infants, of which 81 patients were successfully followed up. In total, 22.2% of patients experienced recurrent wheezing episodes. The proportion of patients with history of eczema, systemic glucocorticoid use and patients with moderate-to-severe disease were significantly higher in the recurrent wheezing group than the non-recurrent wheezing group (83.3% vs 52.4%; 66.7% vs 36.5%; 61.1% vs 33.3%, respectively, all P < 0.05); There were no significant differences between patients with and without recurrent wheezing episodes in the levels of TSLP, IL2, IL13, TIMP-1, MMP-9, IL33, IL5, IL4, IL25, TNF- α and MIP-1α (P > 0.05). Logistic regression analysis showed that history of eczema was an independent risk factor for post-bronchiolitis recurrent wheezing (odds ratio [OR] = 5.622; 95% confidence interval [CI], 1.3-24.9; P = 0.023). CONCLUSION: The incidence of recurrent wheezing among infants after contracting bronchiolitis was 22.2% during a 2-year follow-up. History of eczema was the only independent risk factor identified and no correlation was found between the specific virus and disease severity in children with post-bronchiolitis recurrent wheezing.


Asunto(s)
Bronquiolitis/fisiopatología , Ruidos Respiratorios , Bronquiolitis/virología , China , Citocinas/sangre , Eccema/complicaciones , Femenino , Estudios de Seguimiento , Glucocorticoides/efectos adversos , Humanos , Incidencia , Lactante , Masculino , Recurrencia , Ruidos Respiratorios/etiología , Factores de Riesgo , Suero , Factor de Necrosis Tumoral alfa/sangre
3.
J Infect Public Health ; 14(4): 514-520, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33743374

RESUMEN

BACKGROUND: Streptococcus pneumoniae infection is a leading cause of bacterial meningitis in children with severe sequelae. Cytokines are important molecules in regulating of host inflammatory and anti-inflammatory responses. So far, the cytokine profile of bacterial meningitis caused by single pathogen has been rarely reported. The aim of this study was to explore serum cytokine profile in pediatric patients with pneumococcal meningitis (PM) and its clinical relevance which could be considered as a valuable tool for differential diagnosis of PM. METHODS: During 2015-2018, 95 children with laboratory-confirmed PM were included. Of them, 63 had serum samples at admission. Ten cytokines including TNF-α, IL-12p40, IL-17A, IL-1ß, IFN-γ, GM-CSF, IL-10, CXCL-1, IL-8 and IL-13 were measured by multiplex immunoassay in sera of 63 PM patients and 55 age-matched healthy controls (HCs). Level of serum cytokines was compared with different clinical features of patients. RESULTS: Significantly higher level of IL-10 was observed in patients than HCs (median, 2.19 vs. 1.92 pg/mL, p = 0.017). Significantly lower levels of serum IL-12p40, IL-17A and IL-1ß were observed in patients than HCs (median, 0.68 vs. 10.12 pg/mL, p < 0.0001; 1.14 vs. 1.14 pg/mL, p = 0.004; 1.00 vs. 5.09 pg/mL, p < 0.0001, respectively). No difference was found in levels of other cytokines between patients and controls. A negative correlation was noticed between percentages of blood neutrophils and concentrations of IL-10 (p = 0.048, r = -0.25). Significantly lower levels of IL-12p40 and CXCL-1 were observed in PM patients with sepsis than those without (median 0.68 vs. 1.64 pg/mL, p = 0.026; 7.25 vs. 12.84 pg/mL, p = 0.043, respectively). CONCLUSIONS: Our results suggested that there might be significant changes in serum pro-inflammatory and anti-inflammatory cytokines in PM children and that the determination of these cytokines may have limited value for evaluation of clinical outcome of pediatric PM.


Asunto(s)
Citocinas/sangre , Meningitis Neumocócica/diagnóstico , Niño , Humanos , Laboratorios , Meningitis Neumocócica/sangre , Factor de Necrosis Tumoral alfa/sangre
4.
Infez Med ; 29(1): 1-9, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33664168

RESUMEN

In this review, we summarize the possible mechanisms of COVID-19-associated coagulopathy and compare its features to other similar conditions. The recent COVID-19 pandemic has caused enormous mortality and morbidity worldwide. It is important to note that COVID-19-associated thrombotic events play a huge role in the morbidity of this disease. Interestingly, it has been observed that this complication may occur despite prophylactic anticoagulant therapy. Recent studies on COVID-19-associated coagulopathy revealed that the COVID-19-associated hypercoagulability is more frequently observed among those with a severe course of the disease. Various mechanisms have been suggested as explanations for this condition and possible underlying etiologies.


Asunto(s)
Trastornos de la Coagulación Sanguínea/etiología , /complicaciones , /metabolismo , Anticoagulantes/administración & dosificación , Anticoagulantes/efectos adversos , Trastornos de la Coagulación Sanguínea/metabolismo , Endotelio Vascular/metabolismo , Productos de Degradación de Fibrina-Fibrinógeno/metabolismo , Hemostasis , Heparina/efectos adversos , Humanos , Interleucina-10/sangre , Interleucina-6/sangre , Receptores de Interleucina-2/sangre , Trombofilia/etiología , Trombosis/etiología , Factor de Necrosis Tumoral alfa/sangre , Internalización del Virus
5.
In Vivo ; 35(2): 1295-1298, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33622933

RESUMEN

BACKGROUND/AIM: Lately, studies have reported contradicting results on the cytokine storm seen in critically-ill COVID-19 patients. Depending on the control group used, cytokines have been found to be higher, similar or even lower in COVID-19 compared to critical illnesses associated with elevated cytokine concentrations. However, most of these studies do not take into account critical illness severity. Hence, we decided to compare cytokine levels in critically-ill COVID-19 patients and critically-ill patients of a general intensive care unit (ICU), who did not have sepsis or septic shock, but had an equal disease severity. PATIENTS AND METHODS: Interleukin (IL)-6, IL-8, IL-10 and tumour necrosis factor-α (TNF-α) were measured on ICU admission in mechanically ventilated, COVID-19 (N=36) and non-COVID-19 (N=30) patients, who had not received dexamethasone, and had equal critical illness severity. Non-COVID-19 patients did not have sepsis or septic shock. RESULTS: In our case control study, circulating IL-6 and IL-10 were lower, while TNF-α and IL-8 levels were higher in critically-ill COVID-19 patients, compared to critically-ill non-COVID-19 patients. CONCLUSION: It is difficult to infer whether the cytokine storm seen in COVID-19 differs from other critical conditions. It is important to recognize that the conclusions of related studies may depend on control group selection.


Asunto(s)
/prevención & control , Enfermedad Crítica/terapia , Síndrome de Liberación de Citoquinas/metabolismo , Unidades de Cuidados Intensivos/estadística & datos numéricos , /aislamiento & purificación , Adulto , Anciano , /virología , Estudios de Casos y Controles , Grupos Control , Femenino , Humanos , Interleucina-10/sangre , Interleucina-6/sangre , Interleucina-8/sangre , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Índice de Severidad de la Enfermedad , Factor de Necrosis Tumoral alfa/sangre
6.
Nutr Metab Cardiovasc Dis ; 31(3): 950-960, 2021 03 10.
Artículo en Inglés | MEDLINE | ID: mdl-33546942

RESUMEN

BACKGROUND & AIMS: Vascular function, blood pressure and inflammation are involved in the pathogenesis of major chronic diseases, including both cardiovascular disease (CVD) and mild cognitive impairment (MCI). This study investigated the effects of food anthocyanins on microvascular function, 24-h ambulatory blood pressure (ABP) and inflammatory biomarkers in older adults with MCI. METHODS AND RESULTS: Thirty-one participants with MCI [19 female, 12 male, mean age 75.3 (SD 6.9) years and body mass index 26.1 (SD 3.3) kg/m2], participated in a randomized, controlled, double-blind clinical trial (Australian New Zealand Clinical Trials Registry: ACTRN12618001184268). Participants consumed 250 mL fruit juice daily for 8 weeks, allocated into three groups: a) high dose anthocyanins (201 mg); b) low dose anthocyanins (47 mg); c) control. Microvascular function (Laser Speckle Contrast Imaging combined with a post-occlusive reactive hyperaemia test), 24h ABP and serum inflammatory biomarkers were assessed before and after the nutritional intervention. RESULTS: Participants in the high anthocyanins group had a reduction in serum tumor necrosis factor alpha (TNF-α) (P = 0.002) compared to controls and the low anthocyanins group (all P's > 0.05). Serum IL-6, IL-1ß, c-reactive protein, and parameters of microvascular function and 24h ABP were not altered by any treatment. CONCLUSION: A daily high dose of fruit-based anthocyanins for 8 weeks reduced concentrations of TNF-α in older adults with MCI. Anthocyanins did not alter other inflammatory biomarkers, microvascular function or blood pressure parameters. Further studies with a larger sample size and longer period of follow-up are required to elucidate whether this change in the immune response will alter CVD risk and progression of cognitive decline.


Asunto(s)
Antocianinas/administración & dosificación , Presión Sanguínea , Cognición , Disfunción Cognitiva/dietoterapia , Jugos de Frutas y Vegetales , Mediadores de Inflamación/sangre , Factor de Necrosis Tumoral alfa/sangre , Anciano , Anciano de 80 o más Años , Biomarcadores/sangre , Disfunción Cognitiva/sangre , Disfunción Cognitiva/fisiopatología , Disfunción Cognitiva/psicología , Método Doble Ciego , Regulación hacia Abajo , Femenino , Humanos , Masculino , Microcirculación , Nueva Gales del Sur , Factores de Tiempo , Resultado del Tratamiento
7.
Medicine (Baltimore) ; 100(5): e24275, 2021 Feb 05.
Artículo en Inglés | MEDLINE | ID: mdl-33592871

RESUMEN

ABSTRACT: To investigate serum level of high mobility group box protein-1 (HMGB1) and prognosis of patients with end-stage renal disease (ESRD) on hemodialysis (HD) and peritoneal dialysis (PD).This prospective cohort observational study included a total of 253 ESRD patients who came to our hospital for HD or PD from February 2013 to February 2015. Enzyme linked immunosorbent assay (ELISA) method was used to detect the serum level of HMGB1, interleukin (IL-6), IL-8, and tumor necrosis factor-alpha (TNF-α). The kidney disease quality of life short form (KDQOL-SF) and kidney disease targeted area (KDTA) was applied for evaluating the quality of life. Kaplan-Meier (K-M) curve was performed for survival time.Serum level of HMGB1 in patients on HD was higher than PD. HMGB1 levels were gradually decreased with the treatment of HD or PD. Furthermore, HMGB1 was positively correlated with IL-6 and TNF-α. Moreover, patients with higher HMGB1 had more complications than patients with lower HMGB1, but there was no difference for the survival rate. In addition, the quality of life was associated with different dialysis methods.The serum level of HMGB1 and prognosis of ESRD patients was associated with different dialysis methods.


Asunto(s)
Proteína HMGB1/sangre , Fallo Renal Crónico , Diálisis Peritoneal , Calidad de Vida , Diálisis Renal , China/epidemiología , Correlación de Datos , Femenino , Humanos , Interleucina-6/sangre , Estimación de Kaplan-Meier , Fallo Renal Crónico/sangre , Fallo Renal Crónico/mortalidad , Fallo Renal Crónico/psicología , Fallo Renal Crónico/terapia , Masculino , Persona de Mediana Edad , Diálisis Peritoneal/métodos , Diálisis Peritoneal/estadística & datos numéricos , Pronóstico , Estudios Prospectivos , Diálisis Renal/métodos , Diálisis Renal/estadística & datos numéricos , Factor de Necrosis Tumoral alfa/sangre
8.
Med Clin (Barc) ; 156(7): 332-335, 2021 04 09.
Artículo en Inglés, Español | MEDLINE | ID: mdl-33549331

RESUMEN

INTRODUCTION: Rise of central cytokines resulting from infections produces neuronal changes. Covid-19 allows the study of depressive symptoms in sustained stress and its relationship with molecular mechanisms. OBJECTIVES: To assess correlation between IL-6, IL-1ß and TNF-α and depressive symptoms. Characterize the depressive symptoms present. METHODS: Observational study. Patients admitted for Covid-19 older than 60 years with a interleukin determination were included. The Yesavage Geriatric Depression Scale (GDS) was used, associating each item with a neurotransmitter. RESULTS: 27 patients included. We did not find correlation between IL-6 levels and the GDS scale score (rho=0.204; 95% CI -0.192 to 0.543); with IL-1ß levels (rho=-0.126; 95% CI -0.490 to 0.276); nor of TNF-α (rho=-0.033; 95% CI -0.416 to 0.360). 3 patients (11.1%) presented score compatible with depressive disorder. It was associated with a deficiency of noradrenaline and serotonin. CONCLUSIONS: We found no correlation between the levels of IL-6, IL-1ß, and TNF-α with the GDS score. Depressive symptomatology is similar to vascular depressions.


Asunto(s)
/psicología , Depresión/inmunología , Depresión/virología , Interleucina-1beta/sangre , Interleucina-6/sangre , Factor de Necrosis Tumoral alfa/sangre , Anciano , Anciano de 80 o más Años , Biomarcadores/sangre , /inmunología , Depresión/sangre , Depresión/diagnóstico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Escalas de Valoración Psiquiátrica , Factores de Riesgo
9.
Arch Gynecol Obstet ; 303(3): 631-643, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33439300

RESUMEN

BACKGROUND: Polycystic ovarian syndrome (PCOS) is the most prevalent metabolic disorder in reproductive-age women. It is indeed a multifactorial condition evidenced by ovarian dysfunction, hyperandrogenaemia, infertility, hormonal imbalance and chronic anovulation. Experimental evidence infers that PCOS women are prone to cardiovascular problems and insulin resistance. PURPOSE: To furnish the details about the association of inflammatory markers in PCOS. DESIGN: An extensive literature search on PubMed, science direct and google scholar has been performed for articles about PCOS and inflammation in PCOS. A comprehensive analysis using original articles, reviews, systemic and meta-analysis was conducted for better understanding the relationship between inflammatory cytokines and PCOS. RESULTS: The inflammatory markers perform a substantial part in managing the functions of the ovary. Any disturbances in their levels can lead to ovarian dysfunction. Inflammatory markers are associated with PCOS pathogenesis. The interplay between inflammatory cytokines in the PCOS ovary strongly implies that inflammation is one of the most potent risk factors of PCOS. CONCLUSION: Inflammatory markers have a significant role in regulating the ovary. This manuscript highlights the significance of metabolic and inflammatory markers with PCOS. Since PCOS is always considered as a metabolic disorder, researchers can also consider focusing on the relationship between the inflammatory markers in PCOS to establish a new treatment or management of the disease and to improve women's health.


Asunto(s)
Citocinas/metabolismo , Hiperandrogenismo/complicaciones , Inflamación/sangre , Síndrome del Ovario Poliquístico/sangre , Factor de Necrosis Tumoral alfa/sangre , Anovulación/complicaciones , Biomarcadores/sangre , Femenino , Humanos , Infertilidad , Inflamación/metabolismo , Insulina/sangre , Resistencia a la Insulina , Interleucina-6 , Síndrome Metabólico/complicaciones , Síndrome del Ovario Poliquístico/metabolismo , Síndrome del Ovario Poliquístico/patología
10.
BMJ Open ; 11(1): e043166, 2021 01 27.
Artículo en Inglés | MEDLINE | ID: mdl-33504558

RESUMEN

OBJECTIVE: High blood pressure (BP) is a risk factor for cardiovascular disease. Examining the role of inflammatory mediators on BP is important since vitamin D (VD) is a modifiable risk factor, which possibly modulates inflammatory cytokines. This study simulated what are known as average 'controlled direct effects (CDE)' of inflammatory markers, C reactive protein (CRP), tumour necrosis factor-α (TNF-α), and interlukin-6 (IL-6) on continuous BP measures, while fixing VD, an intermediate variable to specific level. DESIGN: Cross-sectional study. SETTING: We analysed data from the Multi-Community Environment-and-Health Study, 2005-2009, conducted in Eeyou Istchee, Quebec, Canada. PARTICIPANTS: This study recruited 1425 study Indigenous Cree participants from seven Cree communities. Only adults with serum VD levels, inflammatory markers and BP measures were included in this data analysis. PRIMARY AND SECONDARY OUTCOMES MEASURES: Inflammatory markers examined the top 25th exposure percentiles. VD 'insufficiency' (ie, 25-hydroxyvitamin-D levels<50 nmol/L) defined by the Institute of Medicine. CDE for each inflammatory marker in the presence and absence of population VD insufficiency simulated the average direct effect change for systolic and diastolic BP (SBP and DBP) measures. All models were adjusted for exposure-and-mediator outcome relationship. RESULTS: Among 161 participants, 97 (60 %) were female. The prevalence of VD insufficiency was 32%. CDE estimates show in the presence and absence of population vitamin D insufficiency, inflammatory markers have a slightly different association on BP. TNF-α significantly and inversely associated with SBP in the presence of vitamin D insufficiency, fully adjusted model ß = -13.61 (95% CI -24.42 to -2.80); however, TNF-α was not associated with SBP in the absence of vitamin D insufficiency. CRP, IL-6 were also not significantly associated with BP measures, although the magnitude of association was greater for those with elevated inflammation and VD insufficiency. CONCLUSION: This novel analysis shows in the presence of VD insufficiency, inflammation (particularly TNF-α) may affect SBP. Additional research is needed to elucidate these findings, and the temporal relationship between these variables.


Asunto(s)
Hipertensión/sangre , Hipertensión/fisiopatología , Inflamación/sangre , Interleucina-6/sangre , Deficiencia de Vitamina D/etnología , Adolescente , Adulto , Anciano , Presión Sanguínea , Canadá , Niño , Preescolar , Estudios Transversales , Diabetes Mellitus Tipo 2/etnología , Femenino , Humanos , Lactante , Recién Nacido , Inflamación/fisiopatología , Masculino , Persona de Mediana Edad , Quebec/epidemiología , Factor de Necrosis Tumoral alfa/sangre , Vitamina D , Adulto Joven
11.
Int J Mol Sci ; 22(3)2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-33498456

RESUMEN

The urgency of the search for inexpensive and effective drugs with localized action for the treatment of rheumatoid arthritis continues unabated. In this study, for the first time we investigated the Cytos-11 antisense oligonucleotide suppression of TNF-α gene expression in a rat model of rheumatoid arthritis induced by complete Freund's adjuvant. Cytos-11 has been shown to effectively reduce peripheral blood concentrations of TNF-α, reduce joint inflammation, and reduce pannus development. The results achieved following treatment with the antisense oligonucleotide Cytos-11 were similar to those of adalimumab (Humira®); they also compared favorably with those results, which provides evidence of the promise of drugs based on antisense technologies in the treatment of this disease.


Asunto(s)
Antirreumáticos/uso terapéutico , Artritis Reumatoide/tratamiento farmacológico , Oligonucleótidos Antisentido/uso terapéutico , Factor de Necrosis Tumoral alfa/genética , Animales , Silenciador del Gen , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Factor de Necrosis Tumoral alfa/sangre , Factor de Necrosis Tumoral alfa/metabolismo
12.
Int J Nanomedicine ; 16: 443-456, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33505159

RESUMEN

Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs' concentration in isolates from blood (46%; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs' concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that >15 hours post-physical effort, CNVs' concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs' concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.


Asunto(s)
Atletas , Sangre/metabolismo , Nanopartículas/química , Adulto , Recuento de Células Sanguíneas , Proteína C-Reactiva/análisis , Eritrocitos/metabolismo , Femenino , Citometría de Flujo , Humanos , Interleucina-6/sangre , Lípidos/química , Masculino , Persona de Mediana Edad , Esfuerzo Físico/fisiología , Factor de Necrosis Tumoral alfa/sangre , Adulto Joven
13.
Sci Rep ; 11(1): 2291, 2021 01 27.
Artículo en Inglés | MEDLINE | ID: mdl-33504824

RESUMEN

Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients.


Asunto(s)
/sangre , Lipoproteínas HDL/sangre , Apolipoproteína A-I/sangre , Arildialquilfosfatasa/análisis , Arildialquilfosfatasa/sangre , /patología , Estudios de Casos y Controles , Cromatografía Liquida/métodos , Células Endoteliales/patología , Femenino , Francia/epidemiología , Humanos , Masculino , Persona de Mediana Edad , Pandemias , Proteoma/metabolismo , Proteómica/métodos , Proteína Amiloide A Sérica/metabolismo , Espectrometría de Masas en Tándem/métodos , Factor de Necrosis Tumoral alfa/sangre , alfa 1-Antitripsina/sangre
14.
Nat Commun ; 12(1): 213, 2021 01 11.
Artículo en Inglés | MEDLINE | ID: mdl-33431899

RESUMEN

High-fat diet (HFD) decreases insulin sensitivity. How high-fat diet causes insulin resistance is largely unknown. Here, we show that lean mice become insulin resistant after being administered exosomes isolated from the feces of obese mice fed a HFD or from patients with type II diabetes. HFD altered the lipid composition of exosomes from predominantly phosphatidylethanolamine (PE) in exosomes from lean animals (L-Exo) to phosphatidylcholine (PC) in exosomes from obese animals (H-Exo). Mechanistically, we show that intestinal H-Exo is taken up by macrophages and hepatocytes, leading to inhibition of the insulin signaling pathway. Moreover, exosome-derived PC binds to and activates AhR, leading to inhibition of the expression of genes essential for activation of the insulin signaling pathway, including IRS-2, and its downstream genes PI3K and Akt. Together, our results reveal HFD-induced exosomes as potential contributors to the development of insulin resistance. Intestinal exosomes thus have potential as broad therapeutic targets.


Asunto(s)
Dieta Alta en Grasa , Exosomas/metabolismo , Resistencia a la Insulina/genética , Fosfatidilcolinas/metabolismo , Regulación hacia Arriba/genética , Tejido Adiposo/metabolismo , Animales , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patología , Dislipidemias/complicaciones , Dislipidemias/genética , Dislipidemias/patología , Células Epiteliales/metabolismo , Hígado Graso/complicaciones , Hígado Graso/genética , Hígado Graso/patología , Heces , Regulación de la Expresión Génica , Intolerancia a la Glucosa , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Insulina/metabolismo , Interleucina-6/sangre , Intestinos/citología , Lípidos/química , Hígado/metabolismo , Hígado/patología , Activación de Macrófagos , Ratones Endogámicos C57BL , Receptores de Hidrocarburo de Aril/metabolismo , Transducción de Señal , Tetraspanina 30/metabolismo , Factor de Necrosis Tumoral alfa/sangre
15.
Eur Cytokine Netw ; 31(3): 81-93, 2020 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-33361013

RESUMEN

Coronavirus disease (COVID-19) reached pandemic proportions at the beginning of 2020 and continues to be a worldwide concern. End organ damage and acute respiratory distress syndrome are the leading causes of death in severely or critically ill patients. The elevated cytokine levels in severe patients in comparison with mildly affected patients suggest that cytokine release syndrome (CRS) occurs in the severe form of the disease. In this paper, the significant role of pro-inflammatory cytokines, including IL-1, IL-6, and TNF-alpha, and their mechanism of action in the CRS cascade is explained. Potential therapeutic approaches involving anti-IL-6 and anti-TNF-alpha antibodies to fight COVID-19 and reduce mortality rate in severe cases are also discussed.


Asunto(s)
Anticuerpos/uso terapéutico , Síndrome de Liberación de Citoquinas , Interleucina-6/antagonistas & inhibidores , Pandemias , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , /sangre , /tratamiento farmacológico , Síndrome de Liberación de Citoquinas/sangre , Síndrome de Liberación de Citoquinas/tratamiento farmacológico , Síndrome de Liberación de Citoquinas/etiología , Síndrome de Liberación de Citoquinas/mortalidad , Humanos , Interleucina-6/sangre , Factor de Necrosis Tumoral alfa/sangre
16.
BMC Infect Dis ; 20(1): 963, 2020 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-33349241

RESUMEN

BACKGROUND: COVID-19 is highly contagious, and the crude mortality rate could reach 49% in critical patients. Inflammation concerns on disease progression. This study analyzed blood inflammation indicators among mild, severe and critical patients, helping to identify severe or critical patients early. METHODS: In this cross-sectional study, 100 patients were included and divided into mild, severe or critical groups according to disease condition. Correlation of peripheral blood inflammation-related indicators with disease criticality was analyzed. Cut-off values for critically ill patients were speculated through the ROC curve. RESULTS: Significantly, disease severity was associated with age (R = -0.564, P < 0.001), interleukin-2 receptor (IL2R) (R = -0.534, P < 0.001), interleukin-6 (IL-6) (R = -0.535, P < 0.001), interleukin-8 (IL-8) (R = -0.308, P < 0.001), interleukin-10 (IL-10) (R = -0.422, P < 0.001), tumor necrosis factor α (TNFα) (R = -0.322, P < 0.001), C-reactive protein (CRP) (R = -0.604, P < 0.001), ferroprotein (R = -0.508, P < 0.001), procalcitonin (R = -0.650, P < 0.001), white cell counts (WBC) (R = -0.54, P < 0.001), lymphocyte counts (LC) (R = 0.56, P < 0.001), neutrophil count (NC) (R = -0.585, P < 0.001) and eosinophil counts (EC) (R = 0.299, P < 0.001). With IL2R > 793.5 U/mL or CRP > 30.7 ng/mL, the progress of COVID-19 to critical stage should be closely observed and possibly prevented. CONCLUSIONS: Inflammation is closely related to severity of COVID-19, and IL-6 and TNFα might be promising therapeutic targets.


Asunto(s)
/diagnóstico , Inflamación/complicaciones , Adulto , Anciano , Área Bajo la Curva , Proteína C-Reactiva/metabolismo , Estudios Transversales , Femenino , Humanos , Inflamación/inmunología , Interleucina-10/sangre , Interleucina-6/sangre , Interleucina-8/sangre , Recuento de Leucocitos , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , Polipéptido alfa Relacionado con Calcitonina/sangre , Curva ROC , Estudios Retrospectivos , Índice de Severidad de la Enfermedad , Factor de Necrosis Tumoral alfa/sangre
17.
Medicine (Baltimore) ; 99(52): e23832, 2020 Dec 24.
Artículo en Inglés | MEDLINE | ID: mdl-33350771

RESUMEN

ABSTRACT: Guillain-Barré syndrome (GBS) is an acute autoimmune neurological disorder mainly involving the peripheral nerves. Currently, various cytokines have been shown to be involved in the pathogenesis of GBS. Because of their similar biological structures, interleukin (IL)-36α, IL-36ß, IL-36γ, and IL-36 receptor antagonist (Ra) were all renamed and collectively called IL-36 cytokines. The roles of IL-36 cytokines in GBS currently remain unclear.Forty-two patients with GBS and 32 healthy volunteers were included in our study. Serum IL-36α, ß, γ, and interleukin-36 receptor antagonist (IL-36Ra) levels of patients with GBS in the acute and remission phases and healthy volunteers were measured by enzyme-linked immunosorbent assay (ELISA). In addition, we examined the serum levels of other inflammatory factors that have been shown to be involved in GBS pathogenesis, represented by IL-17 and tumor necrosis factor-α (TNF-α). Furthermore, the correlations between the serum levels of IL-36 cytokines and different clinical data or the serum levels of other inflammatory factors in GBS patients were analyzed.Significantly higher serum IL-36α and IL-36γ levels were measured in the acute phase than in the remission phase and in healthy control (HC) subjects (P < .05), while lower serum IL-36Ra levels were measured in the acute phase than in the remission phase and in HC subjects (P < .05). Serum IL-36α and IL-36γ levels were positively correlated with GBS disability scale scores (GDSs), while serum IL-36Ra levels were negatively correlated with GDSs. Correlation analyses among inflammatory factors showed that serum IL-36α and IL-36γ levels in GBS patients were positively correlated with serum IL-17 and TNF-α levels, while serum IL-36Ra levels were negatively correlated with the levels of these 2 inflammatory factors. Similar results were observed in cerebrospinal fluid (CSF), IL-36α and IL-36γ levels in CSF were positively correlated with GDSs, while IL-36Ra levels in CSF were negatively correlated with GDSs. Additionally, the serum and CSF levels of IL-36α and IL-36γ in the axonal subtype of GBS patients were higher than those in the demyelination subtype.Based on our findings, IL-36 cytokines may be involved in the pathogenesis of GBS and some of these cytokines may help predict the disease severity and other clinical characteristics of GBS.


Asunto(s)
Síndrome de Guillain-Barré , Interleucina-1/sangre , Interleucinas/sangre , Adulto , Correlación de Datos , Evaluación de la Discapacidad , Ensayo de Inmunoadsorción Enzimática , Femenino , Síndrome de Guillain-Barré/diagnóstico , Síndrome de Guillain-Barré/inmunología , Síndrome de Guillain-Barré/fisiopatología , Humanos , Interleucina-17/sangre , Masculino , Gravedad del Paciente , Índice de Severidad de la Enfermedad , Factor de Necrosis Tumoral alfa/sangre
18.
Artículo en Inglés | MEDLINE | ID: mdl-33321891

RESUMEN

BACKGROUND: Pyrethroids are synthetic insecticides used for plant protection. They are synthetic analogues of pyrethrins. Lambdacyhalothrin (LCH) is a type II pyrethroid used for wheat, potato, corn farming, and malaria control. There are data that pyrethroids may cause neurotoxicity, nephrotoxicity, hepatotoxicity, and immunotoxicity in non-target organisms. METHODS: The experiment was carried on 32 Albino Swiss mice (16 females and 16 males). The animals were divided into four groups. Controls received canola oil; the rest received LCH orally in oil at a dose of 2 mg/kg bw for 7 days. Memory retention was assessed in a passive avoidance task on day 2 and 7, and spatial memory and motor activity in a Y-maze on day 1 and 7. Blood morphology, biochemical tests, tumor necrosis factor α, and interleukin 1ß were measured. RESULTS: Decreased white blood cell count and red blood cell count, increased creatinine, and increased kidney and liver mass were observed in groups exposed to LCH. In LCH-exposed males' kidneys and livers, interleukin 1ß was significantly elevated, and it was correlated with creatinine concentration. CONCLUSIONS: Subacute poisoning with a low dose of LCH does not significantly affect memory nor locomotor activity but increases proinflammatory interleukin 1ß in male livers and kidneys and reduces white and red blood cell counts.


Asunto(s)
Interleucina-1beta , Nitrilos , Piretrinas , Factor de Necrosis Tumoral alfa , Animales , Recuento de Células Sanguíneas , Células Sanguíneas/efectos de los fármacos , Femenino , Interleucina-1beta/sangre , Locomoción/efectos de los fármacos , Masculino , Memoria/efectos de los fármacos , Ratones , Nitrilos/toxicidad , Plaguicidas/toxicidad , Piretrinas/toxicidad , Factores Sexuales , Factor de Necrosis Tumoral alfa/sangre
19.
PLoS One ; 15(12): e0243545, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33326443

RESUMEN

Downregulation of the T cell system has been proposed as a mechanism to block immunity in colonic cancer (CC). However, little has been studied about circulating αß and γδ T cells and their immunological status in newly diagnosed patients. The aim of this study was to characterize the αß and γδ T cell subsets in peripheral blood of patients with CC matched with healthy volunteers. In this prospective case-control study, blood samples were obtained from 96 patients with newly diagnosed treatment-naïve infiltrating colonic adenocarcinoma and 48 healthy volunteers. Pathological report at surgery was obtained from all CC patients. A significant decrease in CD3+ γδ T cells and CD3+CD8+ γδ T cells (p<0.001) were observed in CC patients. Apoptosis was significantly increased in all conventional and both αß and γδ T cell subsets in patients with CC vs healthy subjects. γδ T cells were decreased in peripheral blood of patients with microscopic infiltration in tissues, history of cancer and synchronous colon cancer (p < 0.05). IFN-γ was significantly reduced in CC patients compared to controls. Cytotoxic effector γδ T cells TEMRA (CD8 and CD56) are the proportionally most abundant T cells in peripheral blood of CC patients. Patients with CC present a deep downregulation in the systemic T-cell immunity. These variations are evident through all tumor stages and suggest that a deficiency in γδ T cell populations could be preventing control of tumor progression. This fact prove the role of immunomodulation on CC carcinogenesis.


Asunto(s)
Neoplasias del Colon/inmunología , Linfocitos Intraepiteliales/inmunología , Anciano , Biomarcadores/sangre , Linfocitos T CD8-positivos/inmunología , Estudios de Casos y Controles , Femenino , Humanos , Interferón gamma/análisis , Interferón gamma/sangre , Linfocitos Intraepiteliales/metabolismo , Masculino , Persona de Mediana Edad , Pronóstico , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Receptores de Antígenos de Linfocitos T gamma-delta/inmunología , Subgrupos de Linfocitos T/inmunología , Factor de Necrosis Tumoral alfa/análisis , Factor de Necrosis Tumoral alfa/sangre
20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 36(10): 903-910, 2020 Oct.
Artículo en Chino | MEDLINE | ID: mdl-33148385

RESUMEN

Objective To investigate the immunotherapeutic effect and mechanism of dendritic cell (DC) vaccine assisted by Tiaohengfang polysaccharides (ThPP) in S180 tumor-bearing mice. Methods Mouse bone marrow-derived cells were cultured in vitro and mature DCs were obtained with the assistance of cytokines and ThPP. The expression of CD80 and CD86 of DCs induced by ThPP was examined, and S180 tumor cells were used as antigens to stimulate dendritic cells to become dendritic cell tumor vaccine. Tumor-bearing models were established in mice by S180 tumor cells inoculated into the armpit of the left forelimb, and the mice were randomly divided into four groups according to body mass, namely tumor-bearing blank group, positive control group (cyclophosphamide), dendritic cell vaccine group adjuvanted by ThPP and TNF-α. The tumor-bearing mice were treated on the 5th and 10th days after inoculation of tumor cells. The tumor-bearing mice were killed on the 12th day and the tumor inhibition was observed by the tumor mass detection. At the same time, peritoneal macrophages were isolated and cultured, and the expression of CD11b and IL-12 were measured by immunohistochemistry. The levels of serum IL-12 and TNF-α in the mice were detected by ELISA. The survival time of the other four groups of tumor-bearing mice was observed after treatment with the same method. Results The expression of CD80 and CD86 in the TNF-α group and ThPP group were higher than those in the blank control group, and the ThPP group was more significant. The tumor inhibition rate and survival extension period of ThPP, TNF-α and positive groups were significantly higher than those of the model blank group. The levels of serum IL-12 and TNF-α in the ThPP group were higher than those in the positive cyclophosphamide group and model black group. There was no significant difference between the ThPP group and TNF-α group. The expression of CD11b in the macrophages of ThPP group was lower than that in the model blank group and positive group, while the expression of IL-12 in the macrophages of ThPP group was higher than that in the model blank group and positive group, without significant difference compared with TNF-α group. Conclusion ThPP-adjuvanted DC tumor vaccine can inhibit tumor growth and prolong survival time of S180 tumor-bearing mice, which is related to promoting the maturation of DCs and increasing the secretion of IL-12 and TNF-α.


Asunto(s)
Vacunas contra el Cáncer/inmunología , Células Dendríticas/inmunología , Medicamentos Herbarios Chinos/farmacología , Neoplasias Experimentales/terapia , Polisacáridos/farmacología , Adyuvantes Inmunológicos/farmacología , Animales , Interleucina-12/sangre , Ratones , Factor de Necrosis Tumoral alfa/sangre
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