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1.
Medicine (Baltimore) ; 99(8): e19287, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32080143

RESUMEN

Little evidence is available about the relationship between ambient temperatures and hypoglycemia in Taiwan. The purpose of the present paper is to investigate whether there is an association between ambient temperatures and hypoglycemia in patients with type 2 diabetes.An ecological study was conducted to analyze the type 2 diabetes dataset of the Taiwan National Health Insurance Program. Every episode of hypoglycemia diagnosed at emergency department among subjects with type 2 diabetes was identified monthly between 2006 and 2013. Average monthly ambient temperatures in Celsius between 2006 and 2013 were measured according to the database of the Central Weather Bureau in Taiwan.The incidence rates of hypoglycemia were higher during the period of cold ambient temperatures (from December to March) than the period of warm ambient temperatures (from April to November). The peak period of hypoglycemia always occurred in winter months (January and February).Patients with type 2 diabetes in Taiwan are more susceptible to hypoglycemia during the period of cold ambient temperatures, particularly in winter months. Clinicians in Taiwan should remind patients to make a preventive strategy for hypoglycemia during the periods of cold ambient temperatures.


Asunto(s)
Frío/efectos adversos , Diabetes Mellitus Tipo 2/epidemiología , Hipoglucemia/epidemiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Bases de Datos Factuales , Servicio de Urgencia en Hospital , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Estaciones del Año , Taiwán/epidemiología , Adulto Joven
2.
Science ; 367(6478): 628-629, 2020 02 07.
Artículo en Inglés | MEDLINE | ID: mdl-32029616
3.
Nature ; 578(7793): 44-45, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-32025006
5.
Chemosphere ; 244: 125513, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32050330

RESUMEN

To achieve efficient biological nutrients removal at low temperature, a modified sequencing batch reactor (SBR) was developed at 10 °C by extending sludge retention time (SRT), shortening aerobic stage and compensating anoxic stage. The average removal rates of ammonium (NH4+-N), total nitrogen (TN) and total phosphorus (TP) were 98.82%, 94.12% and 96.04%, respectively. Variation of carbon source in a typical cycle demonstrated the maximum synthesis of poly-ß-hydroxybutyrate (PHB) (60 mg/L) occurred after feast period. Furthermore, the TP in sludge reached 50.4 mg/g mixed liquor suspended solids (MLSS) (78.4% was inorganic phosphorus and 21.6% was organic phosphorus) after 120 days of operation, indicating an excellent P-accumulating capacity was achieved in this system. Ammonia oxidizing bacteria (AOB) activity inhibition test verified both AOB and ammonia oxidizing archaea (AOA) were involved in ammonia-oxidizing process and the latter accounted for 17%-19%. Metagenomic-based taxonomy revealed the dominant genera were Candidatus Accumulibacter (12.18%), Dechloromonas (7.54%), Haliangium (6.69%) and Candidatus Contendobacter (3.40%). As described from the denitrifying genes perspective, with the exception of nitrite reduction (performed by denitrifiers), denitrifying phosphorus-accumulating organisms (DPAOs) played a leading role in denitrification pathway, showing that poly-ß-hydroxyalkanoates (PHA)-driven nutrients removal was the dominate process.


Asunto(s)
Reactores Biológicos/microbiología , Eliminación de Residuos Líquidos/métodos , Amoníaco/metabolismo , Compuestos de Amonio , Betaproteobacteria/metabolismo , Carbono/metabolismo , Frío , Desnitrificación , Hidroxibutiratos , Metagenoma , Microbiota , Nitritos/metabolismo , Nitrógeno/análisis , Nitrógeno/metabolismo , Fósforo/análisis , Fósforo/metabolismo , Poliésteres , Aguas del Alcantarillado/química , Temperatura Ambiental , Contaminantes del Agua/análisis , Contaminantes del Agua/metabolismo
6.
J Insect Sci ; 20(1)2020 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32104893

RESUMEN

Different densities prerelease packing and times of lethargy in the fruit fly parasitoids Diachasmimorpha longicaudata (Ashmead) were evaluated in order to standardize the process of chilled insect technique for this species. Adults were kept at densities of 0.048, 0.072, 0.096, 0.120, and 0.144 parasitoids/cm2 before release in a México tower, where thermal lethargy was induced at a temperature of 2 ± 2°C for 45 min. Samples of parasitoids were collected to evaluate mortality, survival, fecundity, and flight capacity. All densities showed a similar mortality, both for males (ca. >10%) and females (ca. <7). There was no effect of density on survival and flight capacity in both sexes. On the other hand, fecundity increased with density, 1.66 sons/♀/day, similar to the control. We conclude that a density of 30,000 pupae per cage (0.144 parasitoids/cm2) is adequate for the massive prerelease packaging of the parasitoid D. longicaudata. Regarding the thermal lethargy period, 180 min under 2 ± 2°C conditions, considered as time for management, does not affect the survival, fecundity, and flight capacity of adults. The results obtained are of great utility to establish prerelease packaging parameters for D. longicaudata used in the biological control of Tephritidae fruit fly populations.


Asunto(s)
Frío , Control Biológico de Vectores/métodos , Avispas/fisiología , Animales , Femenino , Fertilidad/fisiología , Vuelo Animal/fisiología , Masculino , Tephritidae/parasitología
7.
Bioresour Technol ; 301: 122744, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31972400

RESUMEN

Vegetated ditches are widely used to treat agricultural wastewater, but effective nitrogen removal at low temperatures remains a challenge because plants wilt in the winter. In this study, three simulated drainage ditches vegetated with Myriophyllum aquaticum were operated with low, medium, and high water levels to study ammonium nitrogen (NH4+-N) removal under cold temperatures. The M. aquaticum ditches had a mean NH4+-N removal efficiency of 75.8-86.8% throughout cold period. Based on nitrogen mass balance, plant uptake, sediment adsorption, and microbial removal accounted for 12.4-21.5%, 0.0-8.1%, and 38.9-54.6% of the influent total nitrogen loading, respectively. The accumulation of nitrate confirmed that intense microbial nitrification occurred in M. aquaticum ditches even at low temperature. These results suggest that M. aquaticum is appropriate as a cold-tolerant plant for NH4+-N removal in drainage ditches.


Asunto(s)
Nitrógeno , Contaminantes Químicos del Agua , Frío , Desnitrificación , Drenaje , Aguas Residuales
8.
9.
Plant Mol Biol ; 102(4-5): 447-462, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31898148

RESUMEN

KEY MESSAGE: ZjICE2 works as a positive regulator in abiotic stress responses and ZjICE2 is a valuable genetic resource to improve abiotic stress tolerance in the molecular breeding program of Zoysia japonica. The basic helix-loop-helix (bHLH) family transcription factors (TFs) play an important role in response to biotic or abiotic stresses in plants. However, the functions of bHLH TFs in Zoysia japonica, one of the warm-season turfgrasses, remain poorly understood. Here, we identified ZjICE2 from Z. japonica, a novel MYC-type bHLH transcription factor that was closely related to ICE homologs in the phylogenetic tree, and its expression was regulated by various abiotic stresses. Transient expression of ZjICE2-GFP in onion epidermal cells revealed that ZjICE2 was a nuclear-localized protein. Also, ZjICE2 bound the MYC cis-element in the promoter of dehydration responsive element binding 1 of Z. japonica (ZjDREB1) using yeast one-hybrid assay. A phenotypic analysis showed that overexpression of the ZjICE2 in Arabidopsis enhanced tolerance to cold, drought, and salt stresses. The transgenic Arabidopsis and Z. japonica accumulated more transcripts of cold-responsive DREB/CBFs and their downstream genes than the wild type (WT) after cold treatment. Furthermore, the transgenic plants exhibited an enhanced Reactive oxygen species (ROS) scavenging ability, which resulted in an efficient maintenance of oxidant-antioxidant homeostasis. In addition, overexpression of the ZjICE2 in Z. japonica displayed intensive cold tolerance with increases in chlorophyll contents and photosynthetic efficiency. Our study suggests that ZjICE2 works as a positive regulator in abiotic stress responses and the ICE-DREB/CBFs response pathway involved in cold stress tolerance is also conserved in Z. japonica. These results provide a valuable genetic resource for the molecular breeding program especially for warm-season grasses as well as other leaf crop plants.


Asunto(s)
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/fisiología , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/fisiología , Poaceae/fisiología , Especies Reactivas de Oxígeno/metabolismo , Estrés Fisiológico , Arabidopsis/genética , Arabidopsis/fisiología , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/fisiología , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Frío , Respuesta al Choque por Frío , Sequías , Filogenia , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/fisiología , Poaceae/genética , Regulón , Tolerancia a la Sal , Factores de Transcripción/genética , Factores de Transcripción/fisiología , Activación Transcripcional
10.
Food Chem ; 312: 125904, 2020 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-31901701

RESUMEN

This study investigated sucrose catabolism during cold-induced sweetening (CIS) and its impact on the quality of sweet potato chips of cultivars with varied levels of tolerance to cold during storage at 6 and 13 °C. In contrast to cultivar Beauregard, cultivar BRS Rubissol and BRS Cuia were cold-sensitive exhibiting intense symptoms of chilling injury at 6 °C. CIS in the sensitive cultivars BRS Rubissol and BRS Cuia was characterized by low accumulation of reducing sugar (RS), high non-reducing sugars content, low invertase activity and high sucrose synthase (SuSy) activity. In the tolerant cultivar Beauregard, the high content of RS was due to high invertases activity. In the three cultivars, the darkening of chips was more influenced by the non-reducing sugars, instead of RS. Our results suggest that SuSy was induced by cold stress in cold-sensitive cultivar, but did not contribute to the CIS in sweet potato.


Asunto(s)
Edulcorantes/química , Metabolismo de los Hidratos de Carbono , Carbohidratos/química , Frío , Glucosiltransferasas , Ipomoea batatas/metabolismo , Edulcorantes/metabolismo , Gusto , beta-Fructofuranosidasa/metabolismo
11.
Microbes Environ ; 35(1)2020.
Artículo en Inglés | MEDLINE | ID: mdl-31996499

RESUMEN

In central Europe, soybean cultivation is gaining increasing importance to reduce protein imports from overseas and make cropping systems more sustainable. In the field, despite the inoculation of soybean with commercial rhizobia, its nodulation is low. In many parts of Europe, limited information is currently available on the genetic diversity of rhizobia and, thus, biological resources for selecting high nitrogen-fixing rhizobia are inadequate. These resources are urgently needed to improve soybean production in central Europe. The objective of the present study was to identify strains that have the potential to increase nitrogen fixation by and the yield of soybean in German soils. We isolated and characterized 77 soybean rhizobia from 18 different sampling sites. Based on a multilocus sequence analysis (MLSA), 71% of isolates were identified as Bradyrhizobium and 29% as Rhizobium. A comparative analysis of the nodD and nifH genes showed no significant differences, which indicated that the soybean rhizobia symbiotic genes in the present study belong to only one type. One isolate, GMF14 which was tolerant of a low temperature (4°C), exhibited higher nitrogen fixation in root nodules and a greater plant biomass than USDA 110 under cold conditions. These results strongly suggest that some indigenous rhizobia enhance biological nitrogen fixation and soybean yield due to their adaption to local conditions.


Asunto(s)
Bradyrhizobium/fisiología , Rhizobium/fisiología , Microbiología del Suelo , Soja/crecimiento & desarrollo , Soja/microbiología , Agricultura , Proteínas Bacterianas/genética , Bradyrhizobium/clasificación , Bradyrhizobium/genética , Bradyrhizobium/metabolismo , Frío , Alemania , Tipificación de Secuencias Multilocus , Fijación del Nitrógeno/genética , Oxidorreductasas/genética , Filogenia , Rhizobium/clasificación , Rhizobium/genética , Rhizobium/metabolismo , Nódulos de las Raíces de las Plantas/microbiología , Estrés Fisiológico , Simbiosis/genética
12.
Food Microbiol ; 87: 103351, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31948609

RESUMEN

The objective of this work was to investigate the effect of stress conditions frequently encountered in food-associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 °C, cold stress) and optimal (37 °C) temperatures and in high NaCl concentrations (6% NaCl, 11 °C; cold-osmotic stress) and tested for their ability to invade the human intestinal epithelial Caco-2 cells. Results demonstrate that the correlation between exposure to cold stress and increased invasion phenotype is strain-dependent as strains investigated exhibited different behaviours, i.e. exposure to cold stress conditions resulted in a significant increase of invasion levels in five out of the eight strains tested, when compared to growth under optimal conditions. On the other hand, when these cold-adapted cells were subsequently submitted to high salt concentrations and low temperature, their enhanced ability to invade Caco-2 was lost. Surprisingly, saturated fatty acids (SFA) and branched chain fatty acids (BCFA) decreased when L. monocytogenes were exposed to stress conditions as opposed to what has been observed in other studies, therefore highlighting that further studies will need to deepen in the understanding of the lipid metabolism of these strains. The effect of stress conditions on the survival of three selected L. monocytogenes strains through an in vitro gastrointestinal (GI) tract digestion model was further investigated. The exposure to cold-osmotic stress increased the survival of one strain through the GI tract.


Asunto(s)
Listeria monocytogenes/fisiología , Listeriosis/microbiología , Adaptación Fisiológica , Células CACO-2 , Frío , Recuento de Colonia Microbiana , Humanos , Listeria monocytogenes/química , Listeria monocytogenes/crecimiento & desarrollo , Listeria monocytogenes/patogenicidad , Presión Osmótica , Cloruro de Sodio/metabolismo , Estrés Fisiológico , Virulencia
13.
Food Microbiol ; 87: 103390, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31948631

RESUMEN

Carcass chilling is a critical step in broiler processing. Understanding the effect of chilling on the bacterial communities of broilers is important, as these communities may be largely responsible for the spoilage process. This study examined the effect of chilling systems (air chilling [AC] and water chilling [WC]) and subsequent aerobic storage on the microbiota of yellow-feathered broiler carcasses using a high-throughput sequencing technique targeting the V3-V4 region of the 16S RNA gene. Evidence of the clear differences in the microbiota structures between AC and WC carcasses was illustrated by principle coordinates and heat map clustered analyses. The distinctions between the AC and WC carcass bacterial communities were more pronounced during the later storage stages. The major genera on the spoiled AC carcasses were Pseudomonas, Psychrobacter and Shewanella, whereas the major genera on the spoiled WC carcasses were Psychrobacter, Pseudomonas and Carnobacterium. These data suggest that the chilling method has a marked effect on the microbiota composition of yellow-feathered broilers along the entire storage period. The chilling method was also of great importance for surface color. However, there was no significant difference in the sensorial shelf-life of chicken when comparing the chilling methods.


Asunto(s)
Carne/microbiología , Microbiota , Animales , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Pollos/microbiología , Frío , Recuento de Colonia Microbiana , Almacenamiento de Alimentos , Humanos , Gusto , Agua/química
14.
Int J Biometeorol ; 64(1): 123-136, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31707494

RESUMEN

Cold- and heat-related mortality poses significant public health concerns worldwide. Although there are numerous studies dealing with the association between extreme ambient temperature and mortality, only a small number adopt a synoptic climatological approach in order to understand the nature of weather systems that precipitate increases in cold- or heat-related mortality. In this paper, the Lamb Weather Type synoptic classification is used to examine the relationship between daily mortality and weather patterns across nine regions of England. Analysis results revealed that the population in England is more susceptible to cold weather. Furthermore, it was found that the Easterly weather types are the most hazardous for public health all-year-long; however, during the cold period, the results are more evident and spatially homogenous. Nevertheless, it is noteworthy that the most dangerous weather conditions are not always associated with extreme (high or low) temperatures, a finding which points to the complexity of weather-related health effects and highlights the importance of a synoptic climatological approach in elucidating the relationship between temperature and mortality.


Asunto(s)
Meteorología , Tiempo (Meteorología) , Animales , Frío , Inglaterra , Calor , Mortalidad , Estaciones del Año , Ovinos
15.
J Sci Food Agric ; 100(3): 1099-1105, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31667840

RESUMEN

BACKGROUND: γ-Aminobutyric acid (GABA) is a non-protein amino acid with several functions in the human body. Although freeze-thawing could effectively accumulate GABA in soybean sprouts, the mechanism has not been revealed. The mechanism by which freeze-thawing enhances GABA accumulation in germinated soybean was revealed by evaluating GABA content, the activity of related synthesis enzymes, and the microstructure of the tissues and cells of sprouts. The germinated soybeans were treated at different temperatures (from -196 °C to 25 °C) for 12 h and then thawed at 25 °C for 6 h. RESULTS: The results showed that GABA content in frozen soybean sprouts did not change significantly before thawing. After thawing, the GABA content of sprouts increased by 83.9% and 82.9% when treated by liquid nitrogen flash freeze at - 80 °C for 12 h compared with the control (4 °C treatment for 12 h). The results indicated that GABA formation mainly occurred during thawing. However, glutamate decarboxylase (GAD), diamine oxidase (DAO), and aminoaldehyde dehydrogenase (AMADH) activity decreased during thawing. Based on the malonaldehyde (MDA) content and microstructure of sprouts, it was suggested that freezing at lower temperatures (< -20 °C) maintained the integrity of the cell structure, while the tissues and cell membranes were broken during thawing. CONCLUSION: These results could provide evidence for the hypothesis that GABA formation resulted from full contact between enzymes and substrates during thawing, rather than the contribution of higher enzyme activity. © 2019 Society of Chemical Industry.


Asunto(s)
Producción de Cultivos/métodos , Semillas/química , Soja/crecimiento & desarrollo , Ácido gamma-Aminobutírico/metabolismo , Amina Oxidasa (conteniendo Cobre)/análisis , Amina Oxidasa (conteniendo Cobre)/metabolismo , Frío , Congelación , Germinación , Glutamato Descarboxilasa/análisis , Glutamato Descarboxilasa/metabolismo , Malondialdehído/análisis , Malondialdehído/metabolismo , Proteínas de Plantas/análisis , Proteínas de Plantas/metabolismo , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Soja/química , Soja/metabolismo , Ácido gamma-Aminobutírico/análisis
16.
Magn Reson Chem ; 58(2): 163-169, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31671221

RESUMEN

The chemical shift difference, Δσ, between the methylene and hydroxyl protons in the high resolution 1 H nuclear magnetic resonance spectrum of ethylene glycol is shown to be pressure dependent. The equilibrium Δσ values for ethylene glycol are reported as a function of temperature and pressure between ambient conditions, 323 K and 2 kbar, respectively. This surface is used along with Δσ values measured in response to a rapid pressure increase to calculate a temperature rise that is used to infer a temperature change for water that is consistent with theoretical estimates. This work implies that compression heating and decompression cooling are not significant enough to interfere with pressure induced protein folding studies.


Asunto(s)
Frío , Glicol de Etileno/química , Calor , Fenómenos Físicos , Presión , Espectroscopía de Protones por Resonancia Magnética
17.
Int J Biometeorol ; 64(3): 397-407, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31720855

RESUMEN

An increasing number of epidemiological studies are finding statistical evidence that diurnal temperature range (DTR) is positively correlated to human morbidity and mortality despite the lack of clear clinical understanding. We examine a 14-year daily time series of emergency department (ED) admissions to the University of Virginia Medical Center in Charlottesville, Virginia, relative to long-term climate records from the Charlottesville/Albemarle County Airport weather station and the Spatial Synoptic Classification. DTR has a consistent strong positive correlation (r ~ 0.5) with maximum temperature in all months but only a weak, negative correlation (r ~- 0.1) with minimum temperature except in late summer (r ~- 0.4). Warm season DTR is highest on dry air mass days with low dew point temperatures. Cool season DTR is unrelated to morning temperature. Using a distributed lag non-linear model with an emphasis on DTR and its seasonal variation, after stratifying the models by season, we find that ED visits are linked to extreme cold events (cold days and nights) and high DTR in the cold season. In the warm season, ED visits are also linked to high DTR, but these are cool, dry, and pleasant days. The existing confusion regarding interpretation of DTR impacts on health might be rectified through a more careful analysis of the underlying physical factors that drive variations in DTR over the course of a year.


Asunto(s)
Frío , Calor , Humanos , Estaciones del Año , Temperatura Ambiental , Virginia
18.
Environ Pollut ; 256: 113433, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31761597

RESUMEN

Epidemiology suggests ambient temperature is the triggers and potential activator of asthma. The role of high and low temperatures on airway inflammation of asthma, and the underlying molecular mechanism are not yet understood. A mouse model of asthma was adopted in our experiment. The BALB/c mice were exposed at different temperature for 4 h (2 h in the morning and 2 h in the afternoon) on weekday. The exposure temperatures were 10 °C, 24 °C and 40 °C. Ovalbumin (OVA) was used to sensitize the mice on days 14, 18, 22, 26, and 30, followed by an aerosol challenge for 30 min from day 32-38. After the final OVA challenge, lung function, serum protein and pulmonary inflammation were assessed. Comparing the OVA with the saline group at 24 °C, we saw a significant increase in: serum Total-IgE (p < 0.05); OVA-sIgE (p < 0.01); IL-4 (p < 0.05); IL-1ß (p < 0.01); IL-6 (p < 0.01); TNF-α (p < 0.01); and the ratio of IL-4/IFN-γ (p < 0.01). At the same time, there was a significant decrease in IFN-γ (p < 0.01). As the temperature increase, there is a U shape for immune proteins and pro-inflammatory factors with a peak value at 24 °C, exception for IFN-γ (inverted U-shape). After the high and low temperature exposure, the Ri and Re increased significantly, while Cldyn decreased significantly compared with the 24 °C group. Histopathological analysis of the OVA groups showed airway remodeling, airway wall thickening and deforming, and subepithelial fibrosis. More obvious changes were found in the high and low temperature exposure groups. The immunohistochemistry suggested that TRPs changed with temperatures. High and low temperatures can aggravate airway inflammation in a mouse model of asthma. TRPs play an important role in temperature aggravation of allergic asthma. The results suggest that asthmatics should avoid exposure to high and low temperatures for too long time.


Asunto(s)
Asma/fisiopatología , Temperatura Ambiental , Animales , Líquido del Lavado Bronquioalveolar , Frío , Modelos Animales de Enfermedad , Femenino , Inflamación , Ratones , Ratones Endogámicos BALB C , Ovalbúmina , Neumonía , Factor de Necrosis Tumoral alfa
19.
Vasa ; 49(2): 133-140, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31808732

RESUMEN

Idiopathic chilblain is a relatively common yet poorly recognized acrosyndrome. This literature review aims to better understand and draw attention to this disorder. Chilblain is a localized inflammation of the skin that occurs on exposure to cold but non-freezing wet weather. It usually resolves spontaneously. The etiology is uncertain, but vasospasm seems to play a role in this abnormal reaction to cold. Diagnosis is most often based on clinical presentation, but a skin biopsy can be useful in dubious cases. In histology, dermal edema and an inflammatory infiltrate are usually present. A distribution of the infiltrate particularly around the eccrine gland is typical. Systemic symptoms and underlying autoimmune disease should be screened. Avoiding cold and keeping extremities warm is the first recommendation for management, as well as smoking cessation. Calcium channel blockers (in particular nifedipine) seems to be the treatment that has been most evaluated in chilblains. However, their effectiveness is not confirmed by all studies. Topical betamethasone is often used but its effect has not been confirmed by randomized clinical trials. Other treatments, such as pentoxifylline, hydrochloroquine and topical nitroglycerin have shown positive effects only in a reduced number of patients. Acupuncture seems to bring a benefit.


Asunto(s)
Eritema Pernio , Biopsia , Frío , Humanos , Piel , Vasoconstricción
20.
Gene ; 727: 144244, 2020 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-31715303

RESUMEN

Sweetpotato (Ipomoea batatas [L.] Lam) is considered an economically important crop worldwide and is used as a source of food, feed, and biomaterials. However, its origin in tropical regions makes it vulnerable to chilling injury during postharvest storage at low temperature. To gain further insight into the molecular mechanism of chilling response, we performed comparative transcriptome analysis of two sweetpotato lines, Xushu 15-1 and Xushu 15-4, with high and low cold storage ability, respectively. Tuberous roots of these lines were stored at 4 °C for 0, 2, and 6 weeks. RNA-Seq data of both lines were de novo assembled, producing 27,636 unigenes with a N50 value of 1204 bp. A total of 525 differentially expressed genes (DEGs) were identified and categorized into six clusters. Genes with higher expression in Xushu 15-1 than in Xushu 15-4 significantly increased in number over time during low temperature storage. Functional annotation of DEGs using KEGG enrichment analysis showed that these DEGs were involved in carbohydrate metabolism, ribosome, protein processing in endoplasmic reticulum, plant-pathogen interaction, and plant hormone signal transduction. Several key candidate genes involved in KEGG pathways were selected and discussed further. The results of this study enhance our understanding of the complex mechanisms involved in low temperature tolerance in sweetpotato during storage and provide a set of candidate genes for the development of new varieties with improved cold storage ability.


Asunto(s)
Respuesta al Choque por Frío/genética , Ipomoea batatas/genética , Frío/efectos adversos , Perfilación de la Expresión Génica/métodos , Regulación de la Expresión Génica de las Plantas/genética , Anotación de Secuencia Molecular/métodos , Raíces de Plantas/genética , Análisis de Secuencia de ARN/métodos , Temperatura Ambiental , Transcriptoma/genética
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