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1.
BMJ Case Rep ; 13(12)2020 Dec 13.
Artículo en Inglés | MEDLINE | ID: mdl-33318282

RESUMEN

This report presents the case of a mixed infection of Actinomyces israelii and Fusobacterium nucleatum, presenting as an extensive neck mass progressing through tissue planes and causing bony destruction. Despite multiple abscess aspirates, imaging and serological investigations, the causative organisms proved elusive over the course of the patient's long admission, only to be identified postdischarge. The patient was successfully initiated on a prolonged course of intravenous antibiotics and did not suffer from any complications. This report aims to raise awareness of the presentation, pathogenicity and treatment of Actinomyces and Fusobacteria infections, given a notable difficulty in diagnosis.


Asunto(s)
Absceso/etiología , Actinomyces/aislamiento & purificación , Coinfección/diagnóstico , Coinfección/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Cuello/patología , Absceso/microbiología , Actinomicosis/diagnóstico , Actinomicosis/microbiología , Administración Intravenosa , Anciano , Antibacterianos/administración & dosificación , Coinfección/tratamiento farmacológico , Infecciones por Fusobacterium/diagnóstico , Infecciones por Fusobacterium/microbiología , Humanos , Masculino , Tomografía Computarizada por Rayos X
3.
Int J Hematol ; 112(6): 851-859, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32880823

RESUMEN

The aim of this study is to investigate the association between chemotherapy-induced fever with neutropenia less than 1500/µL (FwN) and oral bacteria and/or oral immunity in patients with hematological cancer. Thirty-two patients with hematological cancer were enrolled in the study. Secretory immunoglobulin A (sIgA) in saliva and the anaerobic bacteria in tongue coating of each subject were assessed before the first chemotherapy. Eleven subjects had an onset of FwN and 21 subjects did not during the observation periods. It was revealed by the Cox-proportional hazard model analysis that the levels of sIgA were low (HR 0.98, p < 0.05) and the rate of Fusobacterium nucleatum [F. nucleatum count per total bacterial count (%)] was high (HR 1.65, p < 0.05) in patients with FwN onset. Using ROC curve analysis, the optimal cutoff point based on the AUC in the F. nucleatum/sIgA ratio was 0.023, and this model had a 78.4% probability (p < 0.01). The risk of FwN onset was also significantly higher among the group of ≥ 0.023 F. nucleatum/sIgA ratio (HR 66.06, p < 0.01). These results suggest that the rate of F. nucleatum and the levels of sIgA at baseline might be related to FwN onset as risk factors.


Asunto(s)
Antineoplásicos/efectos adversos , Neutropenia Febril/etiología , Neoplasias Hematológicas/tratamiento farmacológico , Neoplasias Hematológicas/inmunología , Inmunoglobulina A Secretora , Boca/inmunología , Boca/microbiología , Adulto , Anciano , Anciano de 80 o más Años , Carga Bacteriana , Femenino , Fusobacterium nucleatum/aislamiento & purificación , Neoplasias Hematológicas/complicaciones , Neoplasias Hematológicas/microbiología , Humanos , Masculino , Persona de Mediana Edad , Factores de Riesgo , Adulto Joven
4.
Medicine (Baltimore) ; 99(15): e19698, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32282725

RESUMEN

BACKGROUND: Periodontal bacteria is the major pathogens in the oral cavity and the main cause of adult chronic periodontitis, but their association with incidence and prognosis in cancer is controversial. The aim of this study was to evaluate the effect of periodontal bacteria infection on incidence and prognosis of cancer. METHODS: A systematic literature search of PubMed, Embase, Web of Science, and Cochrane Library databases was performed to obtain 39 studies comprising 7184 participants. The incidence of cancer was evaluated as odd ratios (OR) with a 95% confidence interval (95% CI) using Review Manager 5.2 software. Overall survival, cancer-specific survival and disease-free survival, which were measured as hazard ratios (HR) with a 95% CI using Review Manager 5.2 software. RESULTS: Our results indicated that periodontal bacteria infection increased the incidence of cancer (OR = 1.25; 95%CI: 1.03-1.52) and was associated with poor overall survival (HR = 1.75; 95% CI: 1.40-2.20), disease-free survival (HR = 2.18; 95%CI: 1.24-3.84) and cancer-specific survival (HR = 1.85, 95%CI: 1.44-2.39). Subgroup analysis indicted that the risk of cancer was associated with Porphyromonas gingivalis (Pg) infection (OR = 2.16; 95%CI: 1.34-3.47) and Prevotella intermedia (Pi) infection (OR = 1.28; 95%CI: 1.01-1.63) but not Tannerella forsythia (Tf) (OR = 1.06; 95%CI: 0.8-1.41), Treponema denticola (Td) (OR = 1.30; 95%CI: 0.99-1.72), Aggregatibacter actinomycetemcomitans (Aa) (OR = 1.00; 95%CI: 0.48-2.08) and Fusobacterium nucleatum (Fn) (OR = 0.61; 95%CI: 0.32-1.16). CONCLUSION: This meta-analysis revealed periodontal bacteria infection increased the incidence of cancer and predicted poor prognosis of cancer.


Asunto(s)
Infecciones Bacterianas/microbiología , Periodontitis Crónica/microbiología , Boca/microbiología , Neoplasias/epidemiología , Aggregatibacter actinomycetemcomitans/aislamiento & purificación , Infecciones Bacterianas/complicaciones , Periodontitis Crónica/complicaciones , Supervivencia sin Enfermedad , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Incidencia , Neoplasias/mortalidad , Porphyromonas gingivalis/aislamiento & purificación , Prevotella intermedia/aislamiento & purificación , Pronóstico , Medición de Riesgo , Treponema denticola/aislamiento & purificación
5.
Medicine (Baltimore) ; 99(10): e18954, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32150046

RESUMEN

RATIONALE: In patients receiving biological therapies, serious infections are a major concern. Infections associated with anti-tumor necrosis factor antibody therapy include tuberculosis, viral, fungal, and bacterial infections. Likewise, severe infections of the upper and lower respiratory tract, lung, skin and soft tissue, urinary tract, gastrointestinal tract, joint, and bone have also been reported previously. However, infections involving the central nervous system are rare, especially an intracranial infection caused by odontogenic infection. To date, only few cases have been reported of this infection. This is the first case of a patient with psoriatic arthritis receiving adalimumab and developing brain abscess of odontogenic origin. PATIENT CONCERNS: A 39-year-old male with psoriatic arthritis receiving adalimumab treatment came to the emergency department with initial presentation of sudden onset convulsions. He had been receiving adalimumab treatment for 1 month. Two days after the third injection, the patient had an episode of sudden-onset general convulsion for nearly 5 min with the upgazing and general tonic presentation. Magnetic resonance imaging (MRI) showed left frontal lobe brain abscess. Pus culture from the brain abscess detected Streptococcus sanguinis (S. sanguinis), Fusobacterium nucleatum (F. nucleatum), and Parvimonas micra (P. micra). DIAGNOSIS: Brain abscess with odontogenic infection. INTERVENTIONS: The patient received left frontal craniotomy, abscess drainage and systemic empiric antibiotics treatment with vancomycin, cefepime, and metronidazole. Due to drug rash with eosinophilia and systemic symptoms during the treatment, vancomycin and metronidazole were discontinued, and systemic antibiotics were switched to teicoplanin and ceftriaxone. OUTCOMES: A brain MRI follow-up performed after 1 month of initial treatment revealed the reduced size of the abscess lesion and minimal oedema. The patient was discharged with stable condition. LESSONS: To the best of our knowledge, this is the first case of a patient with psoriatic arthritis receiving adalimumab and developing brain abscess of odontogenic origin. Such a rare diagnosis must be kept in mind when patients treated with adalimumab present with sudden-onset convulsions. Careful dental examination should be performed before administration of adalimumab.


Asunto(s)
Adalimumab/efectos adversos , Antirreumáticos/efectos adversos , Artritis Psoriásica/tratamiento farmacológico , Absceso Encefálico/diagnóstico , Cerebro , Adulto , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Absceso Encefálico/diagnóstico por imagen , Absceso Encefálico/etiología , Absceso Encefálico/microbiología , Ceftriaxona/administración & dosificación , Ceftriaxona/uso terapéutico , Terapia Combinada , Craneotomía , Diagnóstico Diferencial , Firmicutes/aislamiento & purificación , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Imagen por Resonancia Magnética , Masculino , Periodontitis/complicaciones , Streptococcus/aislamiento & purificación , Streptococcus sanguis , Teicoplanina/administración & dosificación , Teicoplanina/uso terapéutico
6.
PLoS One ; 15(2): e0229485, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32109938

RESUMEN

Periodontal disease, the most prevalent infectious disease in the world, is caused by biofilms formed in periodontal pockets. No specific bacterial species that can cause periodontitis alone has been found in any study to date. Several periodontopathic bacteria are associated with the progress of periodontal disease. Consequently, it is hypothesized that dysbiosis of subgingival microbiota may be a cause of periodontal disease. This study aimed to investigate the relationship between the subgingival microbiota and the clinical status of periodontal pockets in a quantitative and clinically applicable way with the newly developed Oral Care Chip. The Oral Care Chip is a DNA microarray tool with improved quantitative performance, that can be used in combination with competitive PCR to quantitatively detect 17 species of subgingival bacteria. Cluster analysis based on the similarity of each bacterial quantity was performed on 204 subgingival plaque samples collected from periodontitis patients and healthy volunteers. A significant difference in the number of total bacteria, Treponema denticola, Campylobacter rectus, Fusobacterium nucleatum, and Streptococcus intermedia bacteria in any combination of the three clusters indicated that these bacteria gradually increased in number from the stage before the pocket depth deepened. Conversely, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Streptococcus constellatus, which had significant differences only in limited clusters, were thought to increase in number as the pocket depth deepened, after periodontal pocket formation. Furthermore, in clusters where healthy or mild periodontal disease sites were classified, there was no statistically significant difference in pocket depth, but the number of bacteria gradually increased from the stage before the pocket depth increased. This means that quantitative changes in these bacteria can be a predictor of the progress of periodontal tissue destruction, and this novel microbiological test using the Oral Care Chip could be effective at detecting dysbiosis.


Asunto(s)
Bacterias/aislamiento & purificación , ADN Bacteriano/análisis , Microbiota , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Enfermedades Periodontales/microbiología , Bolsa Periodontal/microbiología , Adulto , Campylobacter rectus/aislamiento & purificación , Femenino , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Periodontales/diagnóstico , Índice Periodontal , Porphyromonas gingivalis/aislamiento & purificación , Prevotella intermedia/aislamiento & purificación , Streptococcus constellatus/aislamiento & purificación , Tannerella forsythia/aislamiento & purificación , Treponema denticola/aislamiento & purificación , Adulto Joven
7.
Microbiol Immunol ; 64(2): 99-112, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31793046

RESUMEN

Periodontitis is a major cause of tooth loss in adults that initially results from dental plaque. Subgingival plaque pathogenesis is affected by both community composition and plaque structures, although limited data are available concerning the latter. To bridge this knowledge gap, subgingival plaques were obtained using filter paper (the fourth layer) and curette (the first-third layers) sequentially and the phylogenetic differences between the first-third layers and the fourth layer were characterized by sequencing the V3-V4 regions of 16S rRNA. A total of 11 phyla, 148 genera, and 308 species were obtained by bioinformatic analysis, and no significant differences between the operational taxonomic unit numbers were observed for these groups. In both groups, the most abundant species were Porphyromonas gingivalis and Fusobacterium nucleatum. Actinomyces naeslundii, Streptococcus intermedius, and Prevotella intermedia possessed relatively high proportions in the first-third layers; while in the fourth layer, both traditional pathogens (Treponema denticola and Campylobacter rectus) and novel pathobionts (Eubacterium saphenum, Filifactor alocis, Treponema sp. HOT238) were prominent. Network analysis showed that either of them exhibited a scale-free property and was constructed by two negatively correlated components (the pathogen component and the nonpathogen component), while the synergy in the nonpathogen component was lower in the first-third layers than that in the fourth layer. After merging these two parts into a whole plaque group, the negative/positive correlation ratio increased. With potential connections, the first-third layers and the fourth layer showed characteristic key nodes in bacterial networks.


Asunto(s)
Bacterias/aislamiento & purificación , Placa Dental/microbiología , Microbiota , Periodontitis/microbiología , Actinobacteria/clasificación , Actinobacteria/genética , Actinobacteria/aislamiento & purificación , Actinomyces/aislamiento & purificación , Adulto , Bacterias/clasificación , Bacterias/genética , Clasificación , Femenino , Fusobacterias/clasificación , Fusobacterias/genética , Fusobacterias/aislamiento & purificación , Fusobacterium/aislamiento & purificación , Fusobacterium nucleatum/aislamiento & purificación , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Humanos , Masculino , Metagenómica , Microbiota/genética , Filogenia , Porphyromonas gingivalis/aislamiento & purificación , Prevotella intermedia/aislamiento & purificación , ARN Ribosómico 16S/genética , Spirochaetales/clasificación , Spirochaetales/genética , Spirochaetales/aislamiento & purificación , Streptococcus intermedius/aislamiento & purificación , Treponema/aislamiento & purificación , Adulto Joven
8.
Oral Dis ; 26(1): 122-130, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31710746

RESUMEN

OBJECTIVE: We determined the bacterial community structure of the buccal mucosa in patients with oral lichen planus (OLP) and evaluated the potential association of Fusobacterium nucleatum with OLP. SUBJECTS AND METHODS: We collected buccal mucosal swab samples of patients with OLP (n = 20) and healthy controls (n = 10) and performed 16S rRNA gene sequencing and real-time PCR to determine potentially different bacteria. Damaged and adjacent non-damaged mucosal swab samples of 25 OLP patients were used to detect the amount of F. nucleatum by real-time PCR. RESULTS: Compared with healthy controls, enrichment of Fusobacterium and Granulicatella was more abundant in patients with OLP (p = .0146 and 0.0034). The abundance of Fusobacterium and F. nucleatum was significantly enriched on buccal mucosa of patients with OLP compared with healthy controls (p = .0043 and 0.0235). Compared with adjacent non-damaged buccal mucosa of OLP patients, the amount of F. nucleatum in the damaged mucosa was significantly increased (p = .001). We examined third-level KEGG pathways for bacteria on mucosal surface and found that genes controlling sporulation and ether lipid metabolism were enriched in patients with OLP. CONCLUSIONS: A high amount of F. nucleatum may be associated with OLP. Further studies are required to investigate the precise association of F. nucleatum with OLP.


Asunto(s)
Fusobacterium nucleatum/aislamiento & purificación , Liquen Plano Oral/microbiología , Mucosa Bucal/microbiología , Adulto , Anciano , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Persona de Mediana Edad , ARN Ribosómico 16S/genética
9.
J Pathol ; 250(2): 170-182, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31610014

RESUMEN

Accumulating evidence links Fusobacterium nucleatum with ulcerative colitis (UC). The mechanism by which F. nucleatum promotes intestinal inflammation in UC remains poorly defined. Here, we first examined the abundance and impact of F. nucleatum on disease activity in UC tissues. Next, we isolated a strain of F. nucleatum from UC tissues and explored whether F. nucleatum aggravates the intestinal inflammatory response in vitro and in vivo. We also examined whether F. nucleatum infection involves the NF-κB or IL-17F signaling pathways. Our data showed that F. nucleatum was enriched in 51.78% of UC tissues and was correlated with the clinical course, clinical activity and refractory behavior of UC (p < 0.05). Furthermore, we demonstrated that F. nucleatum promoted intestinal epithelial damage and the expression of the inflammatory cytokines IL-1ß, Il-6, IL-17F and TNF-α. Mechanistically, F. nucleatum targeted caspase activation and recruitment domain 3 (CARD3) through NOD2 to activate the IL-17F/NF-κB pathway in vivo and in vitro. Thus, F. nucleatum orchestrates a molecular network involving CARD3 and IL-17F to control the UC process. Measuring and targeting F. nucleatum and its associated pathways will yield valuable insight into the prevention and treatment of UC. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.


Asunto(s)
Colitis Ulcerosa/microbiología , Infecciones por Fusobacterium/complicaciones , Fusobacterium nucleatum/patogenicidad , Interleucina-17/metabolismo , Proteína Serina-Treonina Quinasa 2 de Interacción con Receptor/biosíntesis , Adolescente , Adulto , Anciano , Animales , Estudios de Casos y Controles , Células Cultivadas , Colitis Ulcerosa/inducido químicamente , Colitis Ulcerosa/metabolismo , Colitis Ulcerosa/patología , Sulfato de Dextran , Modelos Animales de Enfermedad , Femenino , Infecciones por Fusobacterium/metabolismo , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Masculino , Ratones Noqueados , Persona de Mediana Edad , FN-kappa B/metabolismo , ARN Mensajero/genética , Proteína Serina-Treonina Quinasa 2 de Interacción con Receptor/deficiencia , Proteína Serina-Treonina Quinasa 2 de Interacción con Receptor/genética , Proteína Serina-Treonina Quinasa 2 de Interacción con Receptor/fisiología , Índice de Severidad de la Enfermedad , Transducción de Señal/fisiología , Regulación hacia Arriba/fisiología , Adulto Joven
10.
Clin Cancer Res ; 25(20): 6170-6179, 2019 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-31358543

RESUMEN

PURPOSE: Emerging evidence indicates that gut microbiome plays a crucial role in the cancer pathogenesis. Although Fusobacterium nucleatum (F. nucleatum) is associated with poor prognosis in multiple cancers, its clinical significance in predicting response to chemotherapy in patients with esophageal squamous cell carcinoma (ESCC) remains unclear. EXPERIMENTAL DESIGN: The F. nucleatum levels were quantified by qPCR assays in tumor tissues from 551 patients with ESCC from two independent cohorts, including 101 patients who received neoadjuvant chemotherapy prior to curative resection. Associations between F. nucleatum burden and recurrence-free survival (RFS), as well with chemotherapeutic response were evaluated using response evaluation criteria in solid tumors (RECISTs), primary tumor metabolic response defined by maximum standardized uptake value (SUVmax) changes in positron emission tomography-CT (PET/CT), and pathologic tumor regression grade (TRG). RESULTS: High burden of F. nucleatum in patients with ESCC associated with poor RFS in both training [log-rank P = 0.02; HR = 1.61; P = 0.03] and validation cohorts (log-rank P = 0.003; HR = 1.96; P = 0.004). Importantly, patients with ESCC with high levels of F. nucleatum displayed poor chemotherapeutic response for all three evaluation methods: RECIST (P = 0.04), SUVmax change in PET/CT (P = 0.0004), and TRG (P = 0.003). CONCLUSIONS: We conclude that high levels of intratumoral F. nucleatum have a prognostic significance for predicting poor RFS in patients with ESCC. More importantly, our data indicates that higher F. nucleatum burden correlates with poor response to neoadjuvant chemotherapy, suggesting the possibility that an antibiotic intervention against this bacterium may significantly improve therapeutic response in patients with ESCC.


Asunto(s)
Neoplasias Esofágicas/terapia , Carcinoma de Células Escamosas de Esófago/terapia , Esófago/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Terapia Neoadyuvante/métodos , Adulto , Anciano , Anciano de 80 o más Años , Quimioterapia Adyuvante/métodos , ADN Bacteriano/aislamiento & purificación , Supervivencia sin Enfermedad , Neoplasias Esofágicas/microbiología , Neoplasias Esofágicas/mortalidad , Neoplasias Esofágicas/patología , Carcinoma de Células Escamosas de Esófago/microbiología , Carcinoma de Células Escamosas de Esófago/mortalidad , Carcinoma de Células Escamosas de Esófago/patología , Esofagectomía , Esófago/diagnóstico por imagen , Esófago/patología , Esófago/cirugía , Femenino , Fusobacterium nucleatum/genética , Humanos , Masculino , Persona de Mediana Edad , Tomografía Computarizada por Tomografía de Emisión de Positrones , Pronóstico , Criterios de Evaluación de Respuesta en Tumores Sólidos , Estudios Retrospectivos , Factores de Riesgo , Carga Tumoral/efectos de los fármacos
11.
Biomark Med ; 13(9): 725-735, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-31157977

RESUMEN

Aim: The aim of the study was to examine the prevalence and amount of Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) and Streptococcus gallolyticus (Sg) in the saliva of colorectal cancer (CRC) patients and controls. Methods: PCR analyses performed in 71 CRC patients and 77 controls. Results: Saliva samples of patients had higher amounts of Fn (p = 0.001) and Sg (p < 0.001) compared with controls. Amount of Fn and Sg were lower in the microsatellite instability (+) group. Evaluation of salivary Sg amount by receiver operating characteristics analysis found to have diagnostic value for CRC (AUC: 0.84, 95% CI: 0.72-0.96). Conclusion: We found higher amounts of Fn and Sg in the saliva of CRC patients. Salivary Sg could helpful in distinction of CRC.


Asunto(s)
Neoplasias Colorrectales/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Saliva/microbiología , Streptococcus gallolyticus/aislamiento & purificación , Carga Bacteriana , Estudios de Casos y Controles , Neoplasias Colorrectales/diagnóstico , Neoplasias Colorrectales/genética , Disbiosis/complicaciones , Disbiosis/microbiología , Femenino , Fusobacterium nucleatum/genética , Fusobacterium nucleatum/patogenicidad , Microbioma Gastrointestinal/genética , Humanos , Masculino , Inestabilidad de Microsatélites , Persona de Mediana Edad , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/aislamiento & purificación , Porphyromonas gingivalis/patogenicidad , Streptococcus gallolyticus/genética , Streptococcus gallolyticus/patogenicidad
12.
Acta Odontol Scand ; 77(7): 502-507, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31094270

RESUMEN

Objective: The aim of this clinical quality study was to determine whether the aseptic working field is maintained during the endodontic procedure. Materials and methods: Bacterial samples were collected from the rubber dam of 27 patients during endodontic treatment performed by postgraduate students at the Department of Endodontics, University of Oslo. A bacterial sample was first obtained immediately after disinfection of the working field (A), and the second sample was collected just before obturation or dressing with calcium hydroxide cement (B). Aerobic cultivation technique and PCR were used for detection of bacterial growth and species. Results: All samples were negative on culturing except in one case, which showed positive results with cultivation in both sample A and B. Specie detected with cultivation technique were Streptococcus mitis. With PCR technique, 6 samples in 5 patients (11%), showed positive results. Species detected with PCR technique were Bacteroidales spp. Propionibacterium spp., Bacteroidetes spp., Prevotella nigrescens, Haemophilus parainfluenzae, Neisseria elongata, Alloprevotella tannerae, Capnocytophaga granulosa, Cardiobacterium hominis, Fusobacterium nucleatum and Streptococcus mitis. Conclusion: The present study showed that an aseptic working field was maintained throughout the endodontic procedure in 81% (22/27) of the cases after disinfection of the rubber dam.


Asunto(s)
ADN Bacteriano/análisis , Cavidad Pulpar/microbiología , Endodoncia/métodos , Bacterias Gramnegativas/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/diagnóstico , Bacterias Grampositivas/aislamiento & purificación , Infecciones por Bacterias Grampositivas/diagnóstico , Adolescente , Adulto , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Reacción en Cadena de la Polimerasa
13.
PLoS One ; 14(4): e0215088, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30951563

RESUMEN

Colorectal cancer (CRC) is a common and highly lethal form of cancer. Although the etiologic role of Fusobacterium nucleatum (F. nucleatum) in the development of CRC has been elucidated, the specific tumor molecules involved in the progression of CRC induced by F. nucleatum have not been identified. This study investigated several miRNAs and genes involved in the progression of F. nucleatum-induced CRC by Affymetrix miRNA microarray technology and GeneChip Human Transcriptome Array 2.0. The results suggest that miR-4474 and miR-4717 are up-regulated in CRC tissues in response to F. nucleatum infection, compared with the control group (paracancerous tissues), while other genes associated with signaling pathways in cancer, including CREB-binding protein (CREBBP), STAT1, PRKACB, CAMK2B, JUN, TP53 and EWSR1, were dysregulated. Bioinformatic analysis identified CREBBP as the primary aberrantly expressed gene in F. nucleatum-induced CRC. Consistent with the microarray analysis results, real-time RT-PCR analysis demonstrated that the expression of miR-4474/4717 was upregulated while that of CREBBP mRNA was downregulated in CRC patients infected with F. nucleatum. Additionally, CREBBP was identified as a novel target of miR-4474/4717. The results of this study suggest that miR-4474 and miR-4717 are involved in the progression of F. nucleatum-induced CRC by posttranscriptionally regulating the target gene CREBBP.


Asunto(s)
Proteína de Unión a CREB/metabolismo , Neoplasias Colorrectales/genética , Infecciones por Fusobacterium/complicaciones , Fusobacterium nucleatum/aislamiento & purificación , MicroARNs/genética , Adulto , Proteína de Unión a CREB/genética , Estudios de Casos y Controles , Neoplasias Colorrectales/microbiología , Neoplasias Colorrectales/patología , Femenino , Infecciones por Fusobacterium/epidemiología , Infecciones por Fusobacterium/microbiología , Regulación Neoplásica de la Expresión Génica , Humanos , Masculino , Persona de Mediana Edad , Transducción de Señal , Adulto Joven
14.
Eur J Clin Microbiol Infect Dis ; 38(7): 1367-1376, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31025134

RESUMEN

Norway has one of the world's highest incidences of colorectal cancer (CRC). Accumulating research suggests that the intestinal microbiota may have an important role in initiation and progression of colorectal cancer. In order to evaluate microbiome-based biomarkers for non-invasive detection of CRC, the levels of Fusobacterium nucleatum and selected Escherichia coli toxin genes in stool and mucosa from a small cohort of Norwegian patients were investigated. The study cohort included 72 patients scheduled for colonoscopy. The patients were divided into three groups upon their examinations: cancer, polyp, and control groups. Levels of F. nucleatum in stool samples were significantly higher in the cancer group compared with the control group and the polyp group. High levels of F. nucleatum in stool reflected detection of F. nucleatum in the tumor tissues of colorectal cancer patients. However, no difference in the levels of E. coli toxin genes in neither stool nor biopsy samples between the patient groups was observed. This study suggests that a quantitative PCR assay targeting F. nucleatum in stool samples has the potential to be included in a larger panel of biomarkers for non-invasive testing for colorectal cancer.


Asunto(s)
Colon/microbiología , Neoplasias Colorrectales/diagnóstico , Neoplasias Colorrectales/microbiología , Heces/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Anciano , Anciano de 80 o más Años , Biomarcadores de Tumor/análisis , Biopsia , Estudios de Cohortes , Colon/patología , Colonoscopía , Detección Precoz del Cáncer/métodos , Escherichia coli/genética , Femenino , Infecciones por Fusobacterium/complicaciones , Microbioma Gastrointestinal , Humanos , Masculino , Persona de Mediana Edad , Noruega , Reacción en Cadena en Tiempo Real de la Polimerasa
15.
J Infect Chemother ; 25(9): 708-713, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30982727

RESUMEN

PURPOSE: Controversy exists over whether bacterial flora within the appendix differs between patients with and without appendicitis. To examine these potential differences, we cultured the appendiceal luminal microbiota of patients with and without acute appendicitis, and identified the bacterial species therein. METHODS: Fifty-seven patients with acute appendicitis and 37 patients without acute appendicitis who underwent curative resection of colorectal cancer and prophylactic appendectomies (control group) were included. Appendicitis patients were classified into the phlegmonous group or the gangrenous appendicitis group histopathologically. There was no patient with perforated appendicitis. Aerobic isolates were identified using standard identification schemata, and anaerobic isolates were identified according to the Japanese guidelines. RESULTS: There were no significant differences among the three groups in the median number aerobe species present per patient. However, the median number anaerobe species in the gangrenous appendicitis group was significantly higher than that of the control group and the phlegmonous appendicitis group. In addition, the incidence of patients with Bacillus species, Fusobacterium nucleatum, and Bilophila wadsworthia increased as the disease progressed from phlegmonous to gangrenous appendicitis. CONCLUSION: The present results suggest that increased diversity of anaerobes and the translocation of Bacillus species, F. nucleatum, and B. wadsworthia are associated with the progression of acute appendicitis.


Asunto(s)
Apendicitis/microbiología , Apéndice/microbiología , Infecciones Bacterianas/microbiología , Enfermedad Aguda , Adulto , Apendicectomía , Apendicitis/patología , Apendicitis/cirugía , Bacillus/aislamiento & purificación , Bacterias Aerobias/aislamiento & purificación , Bacterias Anaerobias/aislamiento & purificación , Infecciones Bacterianas/patología , Infecciones Bacterianas/cirugía , Bilophila/aislamiento & purificación , Femenino , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Masculino , Microbiota , Persona de Mediana Edad
17.
BMJ Case Rep ; 12(1)2019 Jan 29.
Artículo en Inglés | MEDLINE | ID: mdl-30700472

RESUMEN

Fusobacterium infections can have a wide clinical spectrum, ranging from mild infections to severe sepsis and abscess formation. This range depends partly on the patient's underlying conditions, such as immunosuppression or malignancy. Fusobacteria are commensal rods in the oropharyngeal cavity and digestive tract, but should mucosal barrier disruption occur, in the presence of the above-mentioned predisposing conditions, fusobacteria can spread and cause infections in the soft tissues, liver and so on. An elderly woman was admitted with an altered level of consciousness (lethargy). The ensuing workup revealed a posterior oral cavity tumour (squamous cell carcinoma), Fusobacterium nucleatum bacteraemia and liver abscesses. Due to the severe sepsis, the patient was referred to our intensive care unit, but she passed away despite antibiotic treatment.


Asunto(s)
Bacteriemia/etiología , Infecciones por Fusobacterium/complicaciones , Fusobacterium nucleatum/aislamiento & purificación , Absceso Hepático/etiología , Neoplasias Orofaríngeas/complicaciones , Anciano de 80 o más Años , Resultado Fatal , Femenino , Humanos , Hígado/microbiología
18.
Arch Microbiol ; 201(2): 259-266, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30610246

RESUMEN

The aim of this study is to establish a novel high resolution tracking ability of a specific bacterium in multispecies biofilm. A periodontal multispecies biofilm was constructed with Streptococcus sanguis, Actinomyces naeslundii, Porphyromonas gingivalis and Fusobacterium nucleatum. A single species was stained with fluorescein isothiocyanate (FITC). The mature biofilm was stained for viability (propidium iodide) and analysis was performed with flow cytometry. The sensitivity of the assay was compared with colony forming units (CFU) counts. A single cell suspension of P. gingivalis was grown in broth and biofilm to identify the location of these events on side scatter and forward scatter. The sensitivity of the assay was comparable to that of the CFU counts. The assay allows quantification of the ratio of a single bacterium within the biofilm, and its viable proportion. The described method is reproducible and of high resolution, and allows the examination of microbes' composition and viability within a biofilm structure.


Asunto(s)
Bacterias/aislamiento & purificación , Biopelículas , Citometría de Flujo , Actinomyces/aislamiento & purificación , Fusobacterium nucleatum/aislamiento & purificación , Porphyromonas gingivalis/aislamiento & purificación , Streptococcus sanguis/aislamiento & purificación
19.
Cancer Med ; 8(2): 480-491, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30636375

RESUMEN

The fecal Fusobacterium nucleatum has been reported as a potential noninvasive biomarker for colorectal tumor in several studies, but its exact diagnostic accuracy was ambiguous due to the wide range of sensitivity and specificity. To assess the diagnostic accuracy of fecal F. nucleatum for colorectal tumor, we searched electronic databases including PubMed, Cochrane Library, Embase, and Web of Science, without any date and language restrictions. Two reviewers independently extracted data and appraised study quality with Quality Assessment of Diagnostic Accuracy Studies. We included ten studies comprising 13 cohorts for colorectal cancer (CRC) and seven cohorts for colorectal adenoma (CRA). A total of 1450 patients and 1421 controls for CRC and 656 patients and 827 controls for CRA were included. The pooled sensitivity and specificity of fecal F. nucleatum for CRC were 71% (95% CI, 61%-79%) and 76% (95% CI, 66%-84%), with the area under the receiver-operating characteristics (AUC) curve of 0.80 (95% CI, 0.76-0.83). The pooled sensitivity and specificity of fecal F. nucleatum for CRA were 36% (95% CI, 27%-46%) and 73% (95% CI, 65%-79%), with an AUC of 0.60 (95% CI, 0.56-0.65). Substantial heterogeneity among studies existed, which was partly caused by DNA extraction kits, regions of study, sample size, and demographic characteristics of participants. Fecal F. nucleatum was valuable for the diagnosis of CRC although it performed below expectation. For CRA, the specificity of fecal F. nucleatum indicated the possibility of noninvasive screening. Subgroup analyses for adenoma were incomplete due to lack of data. Heterogeneity limited the credibility of the study.


Asunto(s)
Neoplasias Colorrectales/diagnóstico , Heces/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Biomarcadores , Neoplasias Colorrectales/microbiología , Humanos
20.
Appl Microbiol Biotechnol ; 103(3): 1393-1404, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30470868

RESUMEN

Mounting evidence suggests a causal relationship between specific bacterial infections or microbial compositions and the development of certain malignant neoplasms. In this study, we performed research through 16S rRNA amplicon sequencing, qPCR and fluorescence in situ hybridization to certify the relationship between periodontal pathogens and oral squamous cell carcinoma (OSCC). Subgingival plaque, cancer and paracancerous tissues from 6 patients with OSCC were selected for mapping bacterial profiles by 16S rRNA amplicon sequencing. The research showed that periodontal pathogens were enriched in cancer and paracancerous tissues, while the bacterial profiles were similar between the cancer tissues and subgingival plaque. Furthermore, the relative abundance of Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis was detected in 61 cancer tissues, paracancerous tissues and subgingival plaque samples and in 30 normal tissues by qPCR. The results revealed that P. gingivalis and F. nucleatum existed at higher levels in cancer tissue than in normal tissues and were correlated with subgingival plaques. P. gingivalis was detected using a special oligonucleotide probe in 60.7% of OSCC tissues, 32.8% of paracancerous tissues and 13.3% of normal tissues. Relevance analysis showed that P. gingivalis infection was positively associated with late clinical staging, low differentiation and lymph node metastasis in patients with OSCC, which was accompanied by deeper periodontal pockets, severe clinical attachment loss and loss of teeth. This study revealed that there might be a close relationship between oral microorganisms, particularly periodontal pathogens, and OSCC, which might enrich the pathogenesis of oral squamous carcinoma.


Asunto(s)
Carcinoma de Células Escamosas/microbiología , Fusobacterium nucleatum/aislamiento & purificación , Neoplasias de la Boca/microbiología , Bolsa Periodontal/microbiología , Porphyromonas gingivalis/aislamiento & purificación , Streptococcus sanguis/aislamiento & purificación , Adulto , Anciano , Carcinoma de Células Escamosas/patología , Placa Dental/microbiología , Femenino , Humanos , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Mucosa Bucal/microbiología , Neoplasias de la Boca/patología , ARN Ribosómico 16S/genética
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