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1.
Chem Biol Interact ; 338: 109403, 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33582111

RESUMEN

Cells death is indispensable for embryonic development, tissue homeostasis, and the elimination of cancer, virally infected, or degenerated cells in multicellular organisms. It occurs not only via existing modes but also via unidentified modes, whose elucidation requires. Exposure to non-thermal atmospheric pressure plasma (NTAPP) has been demonstrated to induce cell death, probably because of its ability to generate reactive oxygen species (ROS). However, the mode of this cell death and its underlying mechanism remained elusive. Here we show cell death occurring in a novel and distinctive mode different from apoptosis and necrosis/necroptosis through a mechanism that ROS mediate the loss of the translation inhibitor Programmed cell death 4 (Pdcd4) when cells are cultured in solutions activated by NTAPP irradiation. Thus, our study performed with NTAPP-activated solutions may provide insight into the existence of the atypical cell death in cells and some features of its distinguishing mode and underlying mechanism.


Asunto(s)
Gases em Plasma/farmacología , Biosíntesis de Proteínas , Especies Reactivas de Oxígeno/metabolismo , Proteínas Reguladoras de la Apoptosis , Muerte Celular/efectos de los fármacos , Células HeLa , Humanos , Biosíntesis de Proteínas/efectos de los fármacos , Proteínas de Unión al ARN
2.
Arch Biochem Biophys ; 698: 108726, 2021 02 15.
Artículo en Inglés | MEDLINE | ID: mdl-33326801

RESUMEN

The study investigates the effect of LPDBD (Low Pressure Dielectric Barrier Discharge) (Ar + Air) plasma on seed germination, seedling growth and antioxidant enzyme activity of rice. Rice seeds were treated with LPDBD (Ar + Air) plasma for 2min, 4min, 6min, 8min and 10min. Seed germination rate, seedling growth, total chlorophyll content, enzymatic activity, total soluble sugar and protein concentration were increased in plants grown from the LPDBD (Ar + Air) plasma treated seeds. It was observed that the sprouting of seeds and the growth of seedlings of rice depends on the feed gases used to generate plasma and plasma processing time. In the case of plantlets germinated from the plasma-treated seeds of rice, the H2O2 level was increased significantly both in leaves and roots for 6min, 8min and 10min treatment respectively. No significant change was observed in Nitric Oxide (NO) concentration in seed, leaf, or root of plants grown from LPDBD (Ar + Air) plasma-treated seeds. The amount of total soluble sugar and protein increased significantly in the case of 2min, 4min, 6min, 8min and 10min seed treatment. Although plants exhibited no significant increase in APX activities, but a significant increase of CAT and SOD activity in the leaf and root was found. This study reveals that LPDBD (Ar + Air) plasma is involved in the elevation of ROS species in leaf and root of rice plants which is tightly regulated by the upregulation of CAT activity that ultimately enhances the seed germination and growth of rice plantlets.


Asunto(s)
Catalasa/metabolismo , Oryza/metabolismo , Gases em Plasma/farmacología , Plantones/metabolismo , Semillas/metabolismo , Superóxido Dismutasa/metabolismo , Aire , Argón/química , Germinación/efectos de los fármacos , Germinación/fisiología , Oryza/efectos de los fármacos , Oryza/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Raíces de Plantas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Semillas/efectos de los fármacos , Semillas/crecimiento & desarrollo
3.
Int J Food Microbiol ; 337: 108948, 2021 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-33197682

RESUMEN

Novel processing methods such as cold atmospheric plasma (CAP) and natural antimicrobials like nisin, are of interest to replace traditional food decontamination approaches as, due to their mild nature, they can maintain desirable food characteristics, i.e., taste, texture, and nutritional content. However, the microbial growth characteristics (planktonic growth/surface colonies) and/or the food structure itself (liquid/solid surface) can impact the inactivation efficacy of these novel processing methods. More specifically, cells grown as colonies on a solid(like) surface experience a completely different growth environment to cells grown planktonically in liquid, and thus could display a different response to novel processing treatments through stress adaptation and/or cross protection mechanisms. The order in which combined treatments are applied could also impact their efficacy, especially if the mechanisms of action are complementary. This work presents a fundamental study on the efficacy of CAP and nisin, alone and combined, as affected by food system structure. More specifically, Listeria innocua was grown planktonically (liquid broth) or on a viscoelastic Xanthan gum gel system (1.5% w/v) and treated with CAP, nisin, or a combination of the two. Both the inactivation system, i.e., liquid versus solid(like) surface and the growth characteristics, i.e., planktonic versus colony growth, were shown to impact the treatment efficacy. The combination of nisin and CAP was more effective than individual treatments, but only when nisin was applied before the CAP treatment. This study provides insight into the environmental stress response/adaptation of L. innocua grown on structured systems in response to natural antimicrobials and novel processing technologies, and is a step towards the faster delivery of these food decontamination methods from the bench to the food industry.


Asunto(s)
Antibacterianos/farmacología , Manipulación de Alimentos/métodos , Listeria/efectos de los fármacos , Nisina/farmacología , Gases em Plasma/farmacología , Recuento de Colonia Microbiana , Microbiología de Alimentos , Listeria/crecimiento & desarrollo , Modelos Biológicos , Pasteurización/métodos
4.
J Sci Food Agric ; 101(2): 424-432, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32648588

RESUMEN

BACKGROUND: Pesticides have been widely used to control pests on agricultural products in China, and large amounts of pesticide residues have caused a serious threat to human health. Thus, developing a high-efficiency pesticide degradation method for fresh vegetables represents a great challenge. The present study investigated the effects of dielectric barrier discharge (DBD) plasma on the degradation of malathion and chlorpyrifos in aqueous solutions and on lettuces. RESULTS: DBD treatment significantly degraded malathion and chlorpyrifos in water and on lettuce. After cold plasma treatment at 80 kV for 180 s, the degradation efficiency of malathion (0.5 µg mL-1 ) and chlorpyrifos (1.0 µg mL-1 ) in aqueous solutions reached 64.6% and 62.7%, respectively. The degradation intermediates were explored by HPLC-mass spectrometry and the DBD plasma degradation pathways of malathion and chlorpyrifos were proposed. There was no significant damage to the quality of lettuces, including color and chlorophyll content, after plasma treatment. Ascorbic acid decreased significantly during long-term treatment with DBD plasma. To ensure the quality of lettuces during processing, the treatment time was shortened to 120 s. Under this condition, the degradation efficiency of malathion (0.5 mg kg-1 ) and chlorpyrifos (1.0 mg kg-1 ) on lettuces was found to be 53.1% and 51.4%. More importantly, we noted that cold plasma treatment significantly inactivated the microorganisms on lettuces. CONCLUSION: The results of the present study show that cold plasma is an effective and safe method for the degradation of organic pesticide residues on fresh vegetables at the same time as retaining the original quality. © 2020 Society of Chemical Industry.


Asunto(s)
Cloropirifos/química , Contaminación de Alimentos/prevención & control , Manipulación de Alimentos/métodos , Lechuga/efectos de los fármacos , Malatión/química , Residuos de Plaguicidas/química , Hojas de la Planta/química , Gases em Plasma/farmacología , Contaminación de Alimentos/análisis , Manipulación de Alimentos/instrumentación , Cinética , Lechuga/química , Hojas de la Planta/efectos de la radiación , Contaminantes Químicos del Agua/química
5.
Int J Mol Sci ; 21(24)2020 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-33322775

RESUMEN

Since the earliest agricultural attempts, humankind has been trying to improve crop quality and yields, as well as protect them from adverse conditions. Strategies to meet these goals include breeding, the use of fertilisers, and the genetic manipulation of crops, but also an interesting phenomenon called priming or adaptive response. Priming is based on an application of mild stress to prime a plant for another, mostly stronger stress. There are many priming techniques, such as osmopriming, halopriming, or using physical agents. Non-thermal plasma (NTP) represents a physical agent that contains a mixture of charged, neutral, and radical (mostly reactive oxygen and nitrogen species) particles, and can cause oxidative stress or even the death of cells or organisms upon interaction. However, under certain conditions, NTP can have the opposite effect, which has been previously documented for many plant species. Seed surface sterilization and growth enhancement are the most-reported positive effects of NTP on plants. Moreover, some studies suggest the role of NTP as a promising priming agent. This review deals with the effects of NTP treatment on plants from interaction with seed and cell surface, influence on cellular molecular processes, up to the adaptive response caused by NTP.


Asunto(s)
Adaptación Biológica/efectos de los fármacos , Productos Agrícolas/efectos de los fármacos , Germinación/efectos de los fármacos , Gases em Plasma/farmacología , Estrés Fisiológico/efectos de los fármacos , Adaptación Biológica/genética , Productos Agrícolas/genética , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/efectos de la radiación , Germinación/genética , Estrés Oxidativo , Gases em Plasma/efectos adversos , Gases em Plasma/química , Especies de Nitrógeno Reactivo/metabolismo , Especies de Nitrógeno Reactivo/farmacología , Especies Reactivas de Oxígeno/metabolismo , Especies Reactivas de Oxígeno/farmacología , Semillas , Estrés Fisiológico/genética
6.
PLoS One ; 15(12): e0243965, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33326504

RESUMEN

The response to the COVID-19 epidemic is generating severe shortages of personal protective equipment around the world. In particular, the supply of N95 respirator masks has become severely depleted, with supplies having to be rationed and health care workers having to use masks for prolonged periods in many countries. We sought to test the ability of 7 different decontamination methods: autoclave treatment, ethylene oxide gassing (ETO), low temperature hydrogen peroxide gas plasma (LT-HPGP) treatment, vaporous hydrogen peroxide (VHP) exposure, peracetic acid dry fogging (PAF), ultraviolet C irradiation (UVCI) and moist heat (MH) treatment to decontaminate a variety of different N95 masks following experimental contamination with SARS-CoV-2 or vesicular stomatitis virus as a surrogate. In addition, we sought to determine whether masks would tolerate repeated cycles of decontamination while maintaining structural and functional integrity. All methods except for UVCI were effective in total elimination of viable virus from treated masks. We found that all respirator masks tolerated at least one cycle of all treatment modalities without structural or functional deterioration as assessed by fit testing; filtration efficiency testing results were mostly similar except that a single cycle of LT-HPGP was associated with failures in 3 of 6 masks assessed. VHP, PAF, UVCI, and MH were associated with preserved mask integrity to a minimum of 10 cycles by both fit and filtration testing. A similar result was shown with ethylene oxide gassing to the maximum 3 cycles tested. Pleated, layered non-woven fabric N95 masks retained integrity in fit testing for at least 10 cycles of autoclaving but the molded N95 masks failed after 1 cycle; filtration testing however was intact to 5 cycles for all masks. The successful application of autoclaving for layered, pleated masks may be of particular use to institutions globally due to the virtually universal accessibility of autoclaves in health care settings. Given the ability to modify widely available heating cabinets on hospital wards in well-resourced settings, the application of moist heat may allow local processing of N95 masks.


Asunto(s)
Descontaminación/métodos , Equipo Reutilizado , /virología , /patología , Óxido de Etileno/farmacología , Humanos , Peróxido de Hidrógeno/farmacología , Ácido Peracético/farmacología , Gases em Plasma/farmacología , /aislamiento & purificación , Rayos Ultravioleta , Vesiculovirus/efectos de los fármacos , Vesiculovirus/efectos de la radiación
7.
Int J Food Microbiol ; 335: 108889, 2020 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-33007604

RESUMEN

The safety and quality of cereal grain supplies are adversely impacted by microbiological contamination, with novel interventions required to maximise whole grains safety and stability. The microbiological contaminants of wheat grains and the efficacy of Atmospheric Cold Plasma (ACP) for potential to control these risks were investigated. The evaluations were performed using a contained reactor dielectric barrier discharge (DBD) system; samples were treated for 0-20 min using direct and indirect plasma exposure. Amplicon-based metagenomic analysis using bacterial 16S rRNA gene and fungal 18S rRNA gene with internal transcribed spacer (ITS) region was performed to characterize the change in microbial community composition in response to ACP treatment. The antimicrobial efficacy of ACP against a range of bacterial and fungal contaminants of wheat, was assessed to include individual isolates from grains as challenge pathogens. ACP influenced wheat microbiome composition, with a higher microbial diversity as well as abundance found on the untreated control grain samples. Culture and genomic approaches revealed different trends for mycoflora detection and control. A challenge study demonstrated that using direct mode of plasma exposure with 20 min of treatment significantly reduced the concentration of all pathogens. Overall, reduction levels for B. atrophaeus vegetative cells were higher than for all fungal species tested, whereas B. atrophaeus spores were the most resistant to ACP among all microorganisms tested. Of note, repeating sub-lethal plasma treatment did not induce resistance to ACP in either B. atrophaeus or A. flavus spores. ACP process control could be tailored to address diverse microbiological risks for grain stability and safety.


Asunto(s)
Antiinfecciosos/farmacología , Farmacorresistencia Microbiana , Microbiota/efectos de los fármacos , Gases em Plasma/farmacología , Triticum/microbiología , Bacterias/clasificación , Bacterias/efectos de los fármacos , Bacterias/genética , Bacterias/aislamiento & purificación , Grano Comestible/microbiología , Hongos/clasificación , Hongos/efectos de los fármacos , Hongos/genética , Hongos/aislamiento & purificación , ARN Ribosómico/genética , Especificidad de la Especie
8.
Anticancer Res ; 40(11): 6205-6212, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33109557

RESUMEN

BACKGROUND/AIM: Mammary carcinoma (MC) remains one of the leading causes of morbidity and mortality in the female population worldwide. Cold physical plasma at atmospheric pressure (CAP) has an antioncogenic effect on tumor cells, and its anticancer properties may complement or even extend existing treatment options. In the present study, the efficacy of CAP was characterized on an MC in vitro cell culture system. MATERIALS AND METHODS: MC cells (MCF-7, MDA-MB-231) were directly treated with CAP or incubated with CAP-treated cell culture medium. Cell growth, cell mobility and apoptosis were subsequently analyzed. RESULTS: A single treatment of MC cells with CAP and CAP treated medium led to a treatment-time dependent reduction of cell growth. Furthermore, CAP exposure led to a loss of cellular motility and induced apoptosis. CONCLUSION: Due to its anticancer properties, CAP treatment is an innovative and promising physical approach to expand and complement the treatment options for MC. In particular, a combination of CAP application with surgical and/or chemotherapeutic interventions might significantly improve the therapeutic outcomes.


Asunto(s)
Argón/uso terapéutico , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/patología , Gases em Plasma/uso terapéutico , Argón/farmacología , Presión Atmosférica , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Femenino , Humanos , Cinética , Gases em Plasma/farmacología
9.
Int J Food Microbiol ; 335: 108892, 2020 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-32979616

RESUMEN

Cold plasma is a novel technology for surface decontamination. Almond slices can be contaminated with different microorganisms during their production. In the current research, the atmospheric argon plasma treatment (5, 10, 15, and 20 min) was used to decontamination of almond slices surface. Microbial load, Color, peroxide value, hardness, and sensory properties of slices was comprised with untreated samples (control). Descriptive sensory evaluation about color, flavor, crispiness, crunchiness, and hardness of almond slices were performed. According to the results of the microbial tests, Total count, molds and yeasts, and Staphylococcus aureus of almond surface decreased about 2.95 log cfu/g, 1.81 log cfu/g, and 2.72 log cfu/g after 20 min of plasma treatment, respectively, provided that microbial reduction increased more by increasing the treatment time. Coupled with the color evaluation, peroxide value and sensory attributes didn't change during plasma treatment. Having said that, the hardness of slices was changed by increasing treatment time. Furthermore, Principal Component Analysis and cluster analysis were performed for sensory evaluation. In light of the consumer's point of view, firstly 10 min and secondly 15 min plasma treatment can be more desirable.


Asunto(s)
Argón/farmacología , Descontaminación/métodos , Calidad de los Alimentos , Gases em Plasma/farmacología , Prunus dulcis/microbiología , Recuento de Colonia Microbiana , Microbiología de Alimentos , Hongos/efectos de los fármacos , Hongos/crecimiento & desarrollo , Humanos , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo
10.
Int J Nanomedicine ; 15: 5813-5824, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32821103

RESUMEN

Introduction: This paper presents a novel technique for the synthesis of graphene oxide (GO) with various surface features using high-density atmospheric plasma deposition. Furthermore, to investigate the use of hydrophobic, super-hydrophobic, and hydrophilic graphene in biological applications, we synthesized hydrophobic, super-hydrophobic, and hydrophilic graphene oxides by additional heat treatment and argon plasma treatment, respectively. In contrast to conventional fabrication procedures, reduced graphene oxide (rGO) formed under low pressure and high-temperature environment using a new synthesis method-developed and described in this study-offers a convenient deposition method on any kind surface with controlled wettability. Methods: High density at atmospheric plasma is used for the synthesis of rGO and GO and its biocompatibility based on various wetting properties was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the viability of cells in response to rGO and GO with various surface features was investigated. Structural integrity was characterized by Raman spectroscopy, FESEM and FE-TEM. Wettability was measured via contact angle method and confirmed with XPS analysis. Results: We found that GO coating with a hydrophilic feature is more biocompatible than other surfaces as observed in case of fibroblast cells. We have shown that wettability-controlled by GO deposition-influences biocompatibilities and antibacterial effect of biomaterial surfaces. Discussion: Measuring the contact angle, it is found that contact angle for hydrophobic is increased to 150.590 and reduced to 11.580 by heat and argon plasma treatment, respectively, from 75.880 that was initially in the case of hydrophobic surface. XPS analysis confirmed various oxygen-containing functional groups transforming as deposited hydrophobic surface into superhydrophobic and hydrophilic surface. Thus, we have proposed a new, direct, cost-effective, and highly productive method for the synthesis of rGO and GO-with various surface properties-for biological applications. Similarly, for the dental implant application, the Streptococcus mutans was used as an antibacterial effect and found that S. mutans grows slowly on hydrophilic surface. Thus, antibacterial effect was prominent on GO with hydrophilic surface.


Asunto(s)
Atmósfera/química , Grafito/síntesis química , Gases em Plasma/farmacología , Animales , Muerte Celular/efectos de los fármacos , Línea Celular , Grafito/química , Ratones , Viabilidad Microbiana/efectos de los fármacos , Oxidación-Reducción , Streptococcus mutans/efectos de los fármacos , Agua , Humectabilidad
11.
Anticancer Res ; 40(7): 3743-3749, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32620613

RESUMEN

BACKGROUND/AIM: The antiproliferative effects of cold atmospheric plasma (CAP) make it a promising application option in oncology. The aim of the present study was to examine whether short-term CAP treatment leads to an initial partial elimination of the treated cells or to long-term impairement and inhibition of cell growth. MATERIALS AND METHODS: Cells were treated with CAP and biostatistical modelling was used to estimate growth rates over the incubation time. Four cell lines (U2-OS and MNNG osteosarcoma cells, 3T3 fibroblasts, HaCaT keratinocytes) and three CAP sources (MiniJet-R, kINPen MED, Maxium) were used. RESULTS: The antiproliferative efficacy of CAP was due to a significant reduction in cell count during treatment and the long-lasting inhibition of growth rate in the remaining cells, detectable in all cell lines and after treatment using all three CAP devices. CONCLUSION: Induction of cell death and inhibition of cell growth are part of a general mechanism of biological CAP efficacy. However, data contradict the hypothesis that cancer cells respond more sensitively to CAP treatment compared to non-malignant cells.


Asunto(s)
Proliferación Celular/efectos de los fármacos , Células Plasmáticas/patología , Gases em Plasma/farmacología , Muerte Celular/efectos de los fármacos , Línea Celular , Línea Celular Tumoral , Fibroblastos/efectos de los fármacos , Fibroblastos/patología , Humanos , Cinética , Osteosarcoma/tratamiento farmacológico , Osteosarcoma/patología , Células Plasmáticas/efectos de los fármacos
12.
Arch Oral Biol ; 117: 104822, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32592931

RESUMEN

OBJECTIVE: Overcoming substantial shortcomings of soft liners as physico-chemical changes and liner-biofilm-related infections remains a challenge in the rehabilitation treatment. In this study, protective non-thermal plasma (NTP) treatments were developed on the soft liner surface to improve its surface and physico-chemical properties and to reduce fungal colonization after biofilm inhibition challenge. METHODS: Resinous liner specimens (Coe-Soft) were prepared and distributed in 3 groups according to the surface treatments: (1) untreated (control); (2) treated with sulfur hexafluoride-based NTP (SF6); and (3) treated with hexamethyldisiloxane-based NTP (HMDSO). To test the NTP stability and their protective and antimicrobial effect on the liner surface over time, the morphology, chemical composition, roughness, water contact angle, shore A hardness, sorption and solubility were evaluated before and after the specimens were exposed to dual-species biofilm of Candida albicans and Streptococcus oralis for 14 days. Colony forming units and biofilm structure were assessed. Data were submitted to ANOVA and Tukey tests (α = 0.05). RESULTS: Both treatments modified the surface morphology, increased hydrophobicity and roughness of the liner, and were effective to reduce C. albicans adhesion without affecting the commensal health-associated S. oralis. HMDSO presented chemical stability and lower hardness in both periods, whereas SF6 exhibited higher initial hardness than control and the highest sorption; contrarily, similar solubility was noted for all groups. CONCLUSION: HMDSO-based film showed improved physico-chemical properties and inhibited C. albicans biofilm. Thus, it has potential for use to control candida-related stomatitis and improve liner's stability even after being exposed to biofilm inhibition challenge.


Asunto(s)
Antiinfecciosos , Biopelículas , Alineadores Dentales , Gases em Plasma , Antiinfecciosos/farmacología , Candida albicans , Ensayo de Materiales , Gases em Plasma/farmacología , Streptococcus oralis , Propiedades de Superficie
13.
Arch Biochem Biophys ; 689: 108462, 2020 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-32590068

RESUMEN

Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5 mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) and 10 nm PVA-coated AgNP using U373MG Glioblastoma Multiforme cells. Cytotoxicity in U373MG cells was >100-fold greater when treated with both CAP and PVA-AgNP compared with either therapy alone (IC50 of 4.30 µg/mL with PVA-AgNP alone compared with 0.07 µg/mL after 25s CAP and 0.01 µg/mL 40s CAP). Combined cytotoxicity was ROS-dependent and was prevented using N-Acetyl Cysteine. A novel darkfield spectral imaging method investigated and quantified AgNP uptake in cells determining significantly enhanced uptake, aggregation and subcellular accumulation following CAP treatment, which was confirmed and quantified using atomic absorption spectroscopy. The results indicate that CAP decreases nanoparticle size, decreases surface charge distribution of AgNP and induces uptake, aggregation and enhanced cytotoxicity in vitro.


Asunto(s)
Neoplasias Encefálicas/terapia , Glioblastoma/terapia , Gases em Plasma/farmacología , Plata/farmacología , Neoplasias Encefálicas/metabolismo , Línea Celular Tumoral/metabolismo , Supervivencia Celular/efectos de los fármacos , Glioblastoma/metabolismo , Humanos , Nanopartículas del Metal/análisis , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Plata/farmacocinética
14.
Anticancer Res ; 40(5): 2591-2599, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32366404

RESUMEN

BACKGROUND: Physical plasma is a mixture of reactive particles and electromagnetic radiation. Due to the antimicrobial, immunomodulatory, anti-inflammatory, wound-healing promoting, and antineoplastic effects of body tempered physical plasma under atmospheric pressure (cold atmospheric plasma: CAP), CAP therapy is increasingly becoming the focus of surgical and oncological disciplines. However, when applied in practice, a potential emission of harmful noxae such as toxic nitrogen oxides must be taken into account, which was investigated in the following study. MATERIALS AND METHODS: MiniJet-R Ar CAP device was characterized with respect to NOX-specific spectra, ultraviolet radiation C (UVC) intensity in the range of 200-275 nm and the formation of NOX gases. Instrument-specific parameters such as gas flow, energy setting of the high-frequency generator, and flow rate of the carrier gas Ar were varied. To test the toxic properties of the NO2 concentrations formed by CAP, SK-OV-3 human ovarian cancer cells were incubated with different NO2 concentrations and cell growth was monitored for 120 h. RESULTS: The operation of MiniJet-R led to the formation of NO2 in the proximity of the CAP effluent. Synthesis of NO led to a NO-specific spectrum in the range of 100-275 nm, whereby UVC radiation produced reached intensities of up to 90 mW/m2 NO gas itself, however, was not detectable, as it was converted to NO2 rapidly. Cell culture incubation experiments demonstrated that NO2 in these concentration ranges had no influence on the cell growth of human cancer cells. CONCLUSION: Although no limit values were exceeded in the present study, the emission of high-energy UVC radiation and toxic NO2 is a risk factor with regard to the legal regulations on workplace protection (operator hazard) and the approval of medical devices (patient hazard). This is important for considerations regarding treatment frequency and duration. The growth inhibitory effect of CAP treatment on human cancer cells principally suggests a medical application of the MiniJet-R device, although more extensive studies will have to follow.


Asunto(s)
Presión Atmosférica , Dióxido de Nitrógeno/toxicidad , Gases em Plasma/farmacología , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Humanos , Óxido Nítrico/análisis , Dióxido de Nitrógeno/análisis , Rayos Ultravioleta
15.
Trends Biotechnol ; 38(11): 1278-1291, 2020 11.
Artículo en Inglés | MEDLINE | ID: mdl-32418663

RESUMEN

Viruses can infect all cell-based organisms, from bacteria to humans, animals, and plants. They are responsible for numerous cases of hospitalization, many deaths, and widespread crop destruction, all of which result in an enormous medical, economical, and biological burden. Each of the currently used decontamination methods has important drawbacks. Cold plasma (CP) has entered this field as a novel, efficient, and clean solution for virus inactivation. We present recent developments in this promising field of CP-mediated virus inactivation, and describe the applications and mechanisms of the inactivation. This is particularly relevant because viral pandemics, such as COVID-19, highlight the need for alternative virus inactivation methods to replace, complement, or upgrade existing procedures.


Asunto(s)
Betacoronavirus , Infecciones por Coronavirus/prevención & control , Pandemias/prevención & control , Gases em Plasma/farmacología , Neumonía Viral/prevención & control , Inactivación de Virus , Animales , Bacteriófagos/patogenicidad , Betacoronavirus/patogenicidad , Biotecnología/instrumentación , Infecciones por Coronavirus/transmisión , Descontaminación/métodos , Desinfección/métodos , Microbiología Ambiental , Humanos , Modelos Biológicos , Virus de Plantas/patogenicidad , Gases em Plasma/química , Neumonía Viral/transmisión , Prueba de Estudio Conceptual , Virus/patogenicidad
16.
PLoS One ; 15(5): e0232724, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32374749

RESUMEN

DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.


Asunto(s)
Roturas del ADN de Doble Cadena/efectos de los fármacos , Roturas del ADN de Cadena Simple/efectos de los fármacos , ADN Glicosilasas/metabolismo , Reparación del ADN , Guanina/análogos & derivados , Gases em Plasma/farmacología , Células A549 , Supervivencia Celular/efectos de los fármacos , ADN/metabolismo , Guanina/biosíntesis , Humanos , Estrés Oxidativo/efectos de los fármacos , Especies de Nitrógeno Reactivo/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Regulación hacia Arriba
17.
J Food Prot ; 83(5): 794-800, 2020 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-32318724

RESUMEN

ABSTRACT: Cilantro was recently identified as a vehicle for protozoan illness. Current postharvest practices are not sufficient to inactivate protozoa on cilantro. Cold plasma is an emerging nonthermal waterless technology with potential applications in food processing that are currently being investigated to enhance the safety of herbs. The purpose of this study was to determine the impact of cold atmospheric plasma (CP) on the viability of Cryptosporidium parvum oocysts on cilantro. C. parvum oocysts were inoculated onto cilantro and treated with a CP jet for 0, 30, 90, and 180 s at a working distance of 10 cm with a flow of 1.42 × 10-3 m3/s. Oocyst viability was determined using HCT-8 cell culture infectivity assays. Overall, each treatment significantly reduced oocyst infectivity compared with the 0-s treatment control (P ≤ 0.02). Log inactivations of oocysts observed on cilantro were 0.84, 1.23, and 2.03 for the 30-, 90-, and 180-s treatment times, respectively. Drying and darkening of cilantro leaves was observed with treatments longer than 30 s. CP can reduce C. parvum infectivity on cilantro. With further research and optimization, this treatment technology has potential applications in postharvest processing of cilantro.


Asunto(s)
Coriandrum , Cryptosporidium parvum , Gases em Plasma , Animales , Coriandrum/parasitología , Criptosporidiosis/prevención & control , Cryptosporidium , Cryptosporidium parvum/efectos de los fármacos , Cryptosporidium parvum/crecimiento & desarrollo , Manipulación de Alimentos , Parasitología de Alimentos , Oocistos , Gases em Plasma/farmacología
18.
J Biosci Bioeng ; 130(1): 48-53, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32224010

RESUMEN

Macrolactins (MLNs) have attracted considerable attention due to their antibacterial and antiviral properties. Here, the MLN production of Bacillus sp. strain IMDGX0108 was improved using a breeding strategy of atmospheric room temperature plasma (ARTP) technique. Combining with a selection procedure based on the colony morphology and specific growth rate index (SGRI), two genetically stable mutants A29 and A72 were identified. The MLN production of A29 and A72 was 35.2% and 52.8% greater than that of the parent strain, respectively. The best-performing mutant A72 was subjected to RNA-sequence analysis. Five pathways were significantly enriched, and fatty acid bioprocesses might play an important role in improving the production of MLNs. The combined strategy developed herein (i.e., ARTP mutation plus an efficient screening procedure) might be an appropriate method by which to obtain strains overproducing MLNs.


Asunto(s)
Bacillus/efectos de los fármacos , Bacillus/crecimiento & desarrollo , Alcaloides Indólicos/metabolismo , Gases em Plasma/farmacología , Bacillus/química , Bacillus/genética , Ácidos Grasos/metabolismo , Cinética , Mutación
19.
Sci Rep ; 10(1): 4917, 2020 03 18.
Artículo en Inglés | MEDLINE | ID: mdl-32188896

RESUMEN

Soybean (Glycine max (L.) Merrill) is one of the most important crops worldwide providing dietary protein and vegetable oil. Most of the nitrogen required by the crop is supplied through biological N2 fixation. Non-thermal plasma is a fast, economical, and environmental-friendly technology that can improve seed quality, plant growth, and crop yield. Soybean seeds were exposed to a dielectric barrier discharge plasma operating at atmospheric pressure air with superimposed flows of O2 or N2 as carrying gases. An arrangement of a thin phenolic sheet covered by polyester films was employed as an insulating barrier. We focused on the ability of plasma to improve soybean nodulation and biological nitrogen fixation. The total number of nodules and their weight were significantly higher in plants grown from treated seeds than in control. Plasma treatments incremented 1.6 fold the nitrogenase activity in nodules, while leghaemoglobin content was increased two times, indicating that nodules were fixing nitrogen more actively than control. Accordingly, the nitrogen content in nodules and the aerial part of plants increased by 64% and 23%, respectively. Our results were supported by biometrical parameters. The results suggested that different mechanisms are involved in soybean nodulation improvement. Therefore, the root contents of isoflavonoids, glutathione, auxin and cytokinin, and expansin (GmEXP1) gene expression were determined. We consider this emerging technology is a suitable pre-sowing seed treatment.


Asunto(s)
Fijación del Nitrógeno , Nodulación de la Raíz de la Planta , Gases em Plasma , Nódulos de las Raíces de las Plantas/fisiología , Semillas , Soja/fisiología , Nitrógeno/metabolismo , Fijación del Nitrógeno/efectos de los fármacos , Fenotipo , Desarrollo de la Planta , Nodulación de la Raíz de la Planta/efectos de los fármacos , Gases em Plasma/farmacología , Carácter Cuantitativo Heredable , Nódulos de las Raíces de las Plantas/efectos de los fármacos , Semillas/efectos de los fármacos , Soja/efectos de los fármacos
20.
J Food Sci ; 85(4): 1203-1212, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32118300

RESUMEN

Listeria monocytogenes is often responsible for postprocessing contamination of ready-to-eat (RTE) products including cooked ham. As an emerging technology, atmospheric cold plasma (ACP) has the potential to inactivate L. monocytogenes in packaged RTE meats. The objectives of this study were to evaluate the effect of treatment time, modified atmosphere gas compositions (MAP), ham formulation, and post-treatment storage (1 and 7 days at 4 °C) on the reduction of a five-strain cocktail of L. monocytogenes and quality changes in ham subjected to in-package ACP treatment. Initial average cells population on ham surfaces were 8 log CFU/cm2 . The ACP treatment time and gas composition significantly (P < 0.05) influenced the inactivation of L. monocytogenes, irrespective of ham formulations. When MAP1 (20% O2 + 40% CO2 + 40% N2 ) was used, there was a significantly higher log reduction (>2 log reduction) in L. monocytogenes on ham in comparison to MAP2 (50% CO2 + 50% N2 ) and MAP3 (100% CO2 ), irrespective of ham formulation. Addition of preservatives (that is, 0.1% sodium diacetate and 1.4% sodium lactate) or bacteriocins (that is, 0.05% of a partially purified culture ferment from Carnobacterium maltaromaticum UAL 307) did not significantly reduce cell counts of L. monocytogenes after ACP treatment. Regardless of type of ham, storage of 24 hr after ACP treatment significantly reduced cells counts of L. monocytogenes to approximately 4 log CFU/cm2 . Following 7 days of storage after ACP treatment, L. monocytogenes counts were below the detection limit (>6 log reduction) when samples were stored in MAP1. However, there were significant changes in lipid oxidation and color after post-treatment storage. In conclusion, the antimicrobial efficacy of ACP is strongly influenced by gas composition inside the package and post-treatment storage. PRACTICAL APPLICATION: Surface contamination of RTE ham with L. monocytogenes may occur during processing steps such as slicing and packaging. In-package ACP is an emerging nonthermal technology, which can be used as a postpackaging decontamination step in industrial settings. This study demonstrated the influence of in-package gas composition, treatment time, post-treatment storage, and ham formulation on L. monocytogenes inactivation efficacy of ACP. Results of present study will be helpful to optimize in-package ACP treatment and storage conditions to reduce L. monocytogenes, while maintaining the quality of ham.


Asunto(s)
Embalaje de Alimentos/métodos , Conservación de Alimentos/métodos , Productos de la Carne/microbiología , Gases em Plasma/farmacología , Animales , Bacteriocinas/farmacología , Recuento de Colonia Microbiana , Contaminación de Alimentos/análisis , Embalaje de Alimentos/instrumentación , Conservación de Alimentos/instrumentación , Conservantes de Alimentos/farmacología , Almacenamiento de Alimentos , Listeria monocytogenes/efectos de los fármacos , Listeria monocytogenes/crecimiento & desarrollo , Productos de la Carne/análisis , Lactato de Sodio/farmacología , Porcinos
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