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1.
Food Chem ; 352: 129402, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33690074

RESUMEN

A non-thermal processing method was developed to promote preservation of brown rice using dielectric barrier discharge cold plasma (DBD-CP). Physicochemical properties including free fatty acid (FFA) content, surface color change, volatile organic components (VOCs) and flavor fingerprints were evaluated in brown rice submitted to DBD-CP. FFA levels were 25.2% lower in treated samples compared to the control, and a more stable surface color was obtained at the end of the storage period. A total of 35 major VOCs could be detected in treated samples, and reduced levels of hexanal can be used as an indicator of DBD-CP treatment in brown rice during storage. Moreover, the flavor fingerprints in DBD-CP treated groups can be successfully distinguished through headspace gas chromatography ion mobility spectrometry. Collectively, application of DBD-CP treatment could be utilized as a feasible approach to promote stabilization of brown rice and preserve flavor during storage.


Asunto(s)
Frío , Oryza/química , Gases em Plasma/química , Gusto , Impedancia Eléctrica , Compuestos Orgánicos Volátiles/análisis
2.
Food Chem ; 350: 129195, 2021 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-33636620

RESUMEN

This study investigated the effect of plasma-activated liquid (PAL) including plasma-activated water (PAW) and plasma-activated buffer solution (PABS) for the reduction of chlorothalonil (CTL) and thiram (THM) pesticide residues on tomato fruit. Results revealed that the PAL obtained by using atmospheric air as the feed gas, CTL residues were decreased to 85.3% and 74.2% and THM residues decreased to 79.47 and 72.21% after treatments with PAW10 and PABS10, respectively, and increasing the activation time caused a significant reduction in fungicide residues. In addition, CTL and THM residues were also decreased while increasing the activation time of PAL using Ar/O2 as the feed gas, the concentrations of the CTL residues were decreased to 75.07 and 69.89% for PAW10 and PABS10, respectively and THM residues decreased to 65.89 and 61.91% for PAW10 and PABS10, respectively. Oxidation-reduction potential (ORP) and electrical conductivity (EC) were increased significantly after plasma treatment, while pH values of both solutions were decreased significantly with activation time. NO3- and NO2- concentrations of PAW increased significantly, while for PABS, NO3- concentration decreased but NO2-, with increasing the plasma activation time. Additionally, washing with PAW and PABS caused no notable negative impact on tomato fruit. Results confirmed that PAL treatments showed a significant reduction of CTL and THM fungicide residues (p < 0.05) in tomato without affecting the quality.


Asunto(s)
Frutas/química , Fungicidas Industriales/química , Lycopersicon esculentum/química , Gases em Plasma/química , Agua/química , Tampones (Química) , Residuos de Plaguicidas/análisis , Soluciones
3.
Food Chem ; 349: 129159, 2021 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-33545604

RESUMEN

The effect of dry heat (DH, 130 °C, 1, 3 and 9 h), cold plasma (CP, 40 V, 1, 5 and 10 min) and their combination (D-P) treatment on the structure, physicochemical and digestive properties of red adzuki bean starch were studied. The results showed that DH or CP had slight change in morphology while diffraction pattern of starch was remained. With the extension of treated time of DH and CP, the amylose content, crystallinity, molecular weight, short-range order, the long chain of amylopectin, enthalpy value, swelling power, digestibility were reduced, while gelatinization temperatures, the short chain of amylopectin and solubility were increased. The D-P had deeper modification than the single treatment. The combination of dry heat and cold plasma is a simple and green method to improve the starch structure and enhance starch properties and this modified starch could be implemented to tailor starch to the desired food applications.


Asunto(s)
Frío , Digestión , Calor , Gases em Plasma/química , Almidón/química , Almidón/metabolismo , Vigna/química , Manipulación de Alimentos , Gelatina/química , Peso Molecular , Solubilidad , Termodinámica
4.
Food Chem ; 351: 129278, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33631612

RESUMEN

The poor gel strength and microbial infection of conventional chicken myofibrillar protein (CMP) gels have severely limited the application. Here, plasma activated water (PAW) instead of normal water was used to prepare CMP gels. PAW prepared by treating deionized water with plasma jet was incubated with CMPs and followed by heating to prepare CMP gels. Effects of PAW on CMP gels were assessed in terms of basic physicochemical properties, network structure, and antibacterial activity. The results showed that PAW treatment accelerated the aggregation of CMPs and increased the strength and water holding capacity of CMP gels. Due to the presence of NO and NO2 free radicals in PAW, the prepared CMP gels were endowed with antibacterial activity against Salmonella Enteritidis and Staphylococcus aureus. The new method of PAW-induced CMP gels will have the prospect of improving the quality of gels and extending the shelf life of chicken gel products.


Asunto(s)
Antibacterianos/química , Antibacterianos/farmacología , Pollos , Proteínas Musculares/química , Proteínas Musculares/farmacología , Gases em Plasma/química , Agua/química , Animales , Geles
5.
Food Chem ; 351: 129285, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33640775

RESUMEN

Macro- and microelement determination in chocolate bars by microwave-induced plasma optical emission spectrometry (MIP OES) was evaluated after microwave-assisted sample digestion. Optimization of the sample digestion was carried out, and the recommended conditions were obtained at a temperature of 190 °C, with a digestion time of 40 min and in a mixture constituted by 2.3 mL of nitric acid, 1.0 mL of hydrogen peroxide and 4.7 mL of water. The method was applied in the analysis of chocolate bars, and the concentration ranges of the elements determined were (in mg kg-1): Ca (653-3096); Cr (<0.6-2.8); Cu (<0.16-19.5); Fe (<1.6-227); Mg (147-2775); K (3554-8573); Mn (<0.03-25.2); Na (45.6-1095); Ni (3.2-10.2); P (1111-22594) and Zn (4.8-33.3). The association of the proposed microwave-assisted acid digestion with the MIP OES technique was adequate for multi-element determination in chocolate bars for routine analysis.


Asunto(s)
Chocolate/análisis , Microondas , Gases em Plasma/química , Espectrofotometría/métodos , Digestión , Calor , Peróxido de Hidrógeno/química , Límite de Detección , Metales/análisis , Ácido Nítrico/química , Agua/química
6.
Arch Biochem Biophys ; 700: 108762, 2021 03 30.
Artículo en Inglés | MEDLINE | ID: mdl-33482147

RESUMEN

Non-thermal plasma (NTP) devices generate reactive oxygen species (ROS) and reactive nitrogen species, such as singlet oxygen (1O2), superoxide (O2-), hydroxyl radical (●OH), hydrogen peroxide (H2O2), ozone, and nitric oxide at near-physiological temperature. In preclinical studies, NTP promotes blood coagulation, wound healing with disinfection, and selective killing of cancer cells. Although these biological effects of NTP have been widely explored, the stoichiometric quantitation of ROS in the liquid phase has not been performed in the presence of biocompatible reducing agents, which may modify the final biological effects of NTP. Here, we utilized electron paramagnetic resonance spectroscopy to quantitate ●OH, using a spin-trapping probe 5,5-dimethyl-1-pyrroline-N-oxide; 1O2, using a fluorescent probe; and O2- and H2O2, using luminescent probes, after NTP exposure in the presence of antioxidants. l-ascorbate (Asc) at 50 µM concentration (physiological concentration in serum) significantly scavenged ●OH, whereas (-)-epigallocatechin gallate (EGCG) and α-tocopherol were also effective at performing scavenging activities at 250 µM concentrations. Asc significantly scavenged O2- and H2O2 at 100 µM. l-Dehydroascorbate (DHA), an oxidized form of Asc, degraded H2O2, whereas it did not quench ●OH or O2-, which are sources of H2O2. Furthermore, EGCG efficiently scavenged NTP-induced 1O2, O2-, and H2O2 in Chelex-treated water. These results indicate that the redox cycling of Asc/DHA and metabolites of DHA are important to be considered when applying NTP to cells and tissues. Additionally, ROS-reducing compounds, such as EGCG, affect the outcome. Further studies are warranted to elucidate the interaction between ROS and biomolecules to promote the medical applications of NTP.


Asunto(s)
Ácido Deshidroascórbico/química , Peróxido de Hidrógeno/química , Gases em Plasma/química , Oxidación-Reducción
7.
Molecules ; 26(2)2021 Jan 08.
Artículo en Inglés | MEDLINE | ID: mdl-33430078

RESUMEN

This work presents the long-term determination accuracy study of ICP-QMS for rare earth elements (REEs) in geological matrices. Following high-pressure closed acidic decomposition, REEs are measured repetitively across seven months by ICP-QMS. Under optimum experimental conditions (including spray chamber temperature, gas flow rate, sampling depth, etc.), the REE contents in geological standard materials from basic (basalt BCR-2 and BE-N) to intermediate (andesite AGV-2) and up to acidic (granite GSR-1) show good agreement with the certified values, giving relative errors below 10%. Here, the influence of two storage materials (perfluoroalkoxy PFA and polypropylene PP) on the long-term determination accuracy of REEs has also been monitored. It is found that the relative errors of REEs using a PFA container range from -6.6 to 6.3% (RSDs < 6.0%), while that using a PP container are within -4.0 to 3.9% (RSDs < 4.6%). By using PP material as a solution storage container, the accuracy of REEs quantification in a series of real geological samples are checked, showing the RSDs of less than 5.0%. This work first clarifies the long-term stability of REEs quantification by ICP-QMS covering two types of storage materials, confirming the reasonability of PP material as a daily storage container in terms of higher data precision and lower cost.


Asunto(s)
Metales de Tierras Raras/química , Gases em Plasma/química , Espectrometría de Masas , Análisis Espectral
8.
Food Chem ; 346: 128974, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33465571

RESUMEN

The effects of argon and nitrogen cold plasma treatments on the lipolytic enzymes activity in wheat germ were investigated. Using argon as plasma gas, the residual activity of lipase and lipoxygenase decreased to 42.50% and 87.72%, respectively after 30 min. Switching plasma input gas to nitrogen, the residual activities of lipase and lipoxygenase after the same time of atmospheric cold plasma (ACP) treatment were 77.50% and 92.52%, respectively. The antioxidant potential and phenolic compounds show no significant difference during ACP duration. However, the remaining activities of lipase and lipoxygenase after 30 min steam autoclaving were 6.25% and 18.60%, respectively. Also, the antioxidant activity and total phenolic content reduced by 14.70% and 30.80%, respectively. In brief, the ACP treatment efficiency was function of the input gas and the treatment time. The presented results about the input gas impacts would be useful in industrial development of ACP application for wheat germ stabilization.


Asunto(s)
Lipasa/metabolismo , Lipooxigenasa/metabolismo , Gases em Plasma/química , Triticum/enzimología , Antioxidantes/química , Argón/química , Nitrógeno/química , Fenoles/química , Temperatura , Triticum/química
9.
ACS Appl Mater Interfaces ; 13(3): 3631-3644, 2021 Jan 27.
Artículo en Inglés | MEDLINE | ID: mdl-33448783

RESUMEN

Three-dimensional (3D) scaffolds with optimum physicochemical properties are able to elicit specific cellular behaviors and guide tissue formation. However, cell-material interactions are limited in scaffolds fabricated by melt extrusion additive manufacturing (ME-AM) of synthetic polymers, and plasma treatment can be used to render the surface of the scaffolds more cell adhesive. In this study, a hybrid AM technology, which combines a ME-AM technique with an atmospheric pressure plasma jet, was employed to fabricate and plasma treat scaffolds in a single process. The organosilane monomer (3-aminopropyl)trimethoxysilane (APTMS) and a mixture of maleic anhydride and vinyltrimethoxysilane (MA-VTMOS) were used for the first time to plasma treat 3D scaffolds. APTMS treatment deposited plasma-polymerized films containing positively charged amine functional groups, while MA-VTMOS introduced negatively charged carboxyl groups on the 3D scaffolds' surface. Argon plasma activation was used as a control. All plasma treatments increased the surface wettability and protein adsorption to the surface of the scaffolds and improved cell distribution and proliferation. Notably, APTMS-treated scaffolds also allowed cell attachment by electrostatic interactions in the absence of serum. Interestingly, cell attachment and proliferation were not significantly affected by plasma treatment-induced aging. Also, while no significant differences were observed between plasma treatments in terms of gene expression, human mesenchymal stromal cells (hMSCs) could undergo osteogenic differentiation on aged scaffolds. This is probably because osteogenic differentiation is rather dependent on initial cell confluency and surface chemistry might play a secondary role.


Asunto(s)
Células Madre Mesenquimatosas/citología , Gases em Plasma/química , Ingeniería de Tejidos/métodos , Andamios del Tejido/química , Adhesión Celular , Línea Celular , Proliferación Celular , Humanos , Osteogénesis , Silanos/química , Compuestos de Vinilo/química , Humectabilidad
10.
Food Chem ; 340: 128198, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33032147

RESUMEN

In this study, potential of dielectric-barrier discharge (DBD) plasma treatment (40 kV, 12 kHz at 1, 2, 3 and 4 min) to eliminate soybean agglutinin (SBA) activity was investigated in a SBA model system and soymilk. The plasma treatment decreased the SBA in the model system and hemagglutination activity was decreased by 87.31%. SDS-PAGE analysis confirmed the degradation of the SBA polypeptide chain. The multi-spectroscopic analysis revealed a two-stage structure alteration in the SBA upon exposure to the plasma treatment. Oxidation of NH-/NH2- at the peptide bond disrupted the hydrogen bonds and altered the secondary structure of SBA. Further oxidation of aromatic amino acid, cleavage of peptide bonds and the breakage of polypeptide led to the SBA fragmentation and complete unfolding of the protein. The SBA inactivation by the plasma treatment was confirmed in soymilk. Plasma treatment is a promising technology for the elimination of SBA in soybean product.


Asunto(s)
Lectinas de Plantas/química , Gases em Plasma/química , Proteínas de Soja/química , Impedancia Eléctrica , Enlace de Hidrógeno , Oxidación-Reducción , Estructura Secundaria de Proteína , Leche de Soja/química
11.
Molecules ; 25(24)2020 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-33371281

RESUMEN

Industries' air pollution causes serious challenges to modern society, among them exhaust gases from internal combustion engines, which are currently one of the main sources. This study proposes a non-thermal plasma (NTP) system for placement in the exhaust system of internal combustion engines to reduce the toxic contaminants (HC, CO, and NOx) of exhaust gases. This NTP system generates a high-voltage discharge that not only responds to the ion chemical reaction to eliminate NOx and CO, but that also generates a combustion reaction at the local high temperature of plasma to reduce HC. The NTP system was designed on both the front and rear of the exhaust pipe to analyze the difference of different exhaust flow rates under the specified frequency. The results indicate that the NTP system can greatly reduce toxic contaminants. The NTP reactor placed in the front of exhaust pipe gave HC and CO removal efficiency of about 34.5% and 16.0%, respectively, while the NTP reactor placed in the rear of exhaust pipe gave NOx removal efficiency of about 41.3%. In addition, the voltage and material directly affect the exhaust gases obviously. In conclusion, the proposed NTP system installed in the exhaust system can significantly reduce air pollutants. These results suggest that applying NTP to the combustion engine should be a useful tool to simultaneously reduce both emissions of NOx and CO.


Asunto(s)
Contaminantes Atmosféricos/química , Gases em Plasma/química , Emisiones de Vehículos/análisis , Contaminación del Aire/análisis , Gases/química , Gasolina/análisis , Óxidos de Nitrógeno/química , Material Particulado/química , Fenómenos Físicos
12.
Int J Mol Sci ; 21(24)2020 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-33322775

RESUMEN

Since the earliest agricultural attempts, humankind has been trying to improve crop quality and yields, as well as protect them from adverse conditions. Strategies to meet these goals include breeding, the use of fertilisers, and the genetic manipulation of crops, but also an interesting phenomenon called priming or adaptive response. Priming is based on an application of mild stress to prime a plant for another, mostly stronger stress. There are many priming techniques, such as osmopriming, halopriming, or using physical agents. Non-thermal plasma (NTP) represents a physical agent that contains a mixture of charged, neutral, and radical (mostly reactive oxygen and nitrogen species) particles, and can cause oxidative stress or even the death of cells or organisms upon interaction. However, under certain conditions, NTP can have the opposite effect, which has been previously documented for many plant species. Seed surface sterilization and growth enhancement are the most-reported positive effects of NTP on plants. Moreover, some studies suggest the role of NTP as a promising priming agent. This review deals with the effects of NTP treatment on plants from interaction with seed and cell surface, influence on cellular molecular processes, up to the adaptive response caused by NTP.


Asunto(s)
Adaptación Biológica/efectos de los fármacos , Productos Agrícolas/efectos de los fármacos , Germinación/efectos de los fármacos , Gases em Plasma/farmacología , Estrés Fisiológico/efectos de los fármacos , Adaptación Biológica/genética , Productos Agrícolas/genética , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/efectos de la radiación , Germinación/genética , Estrés Oxidativo , Gases em Plasma/efectos adversos , Gases em Plasma/química , Especies de Nitrógeno Reactivo/metabolismo , Especies de Nitrógeno Reactivo/farmacología , Especies Reactivas de Oxígeno/metabolismo , Especies Reactivas de Oxígeno/farmacología , Semillas , Estrés Fisiológico/genética
13.
PLoS One ; 15(8): e0237604, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32790732

RESUMEN

This study investigated the efficiency of a portable nitric oxide (NO) inhalation device through optimizing its design and structure. The portable rescue device could be used in clinical applications in outbreaks of viral pneumonia such as SARS. To reduce energy consumption for battery-powered portable usage, NO micro-channel plasma reactions induced by a continuous discharge arc were employed. A single-use airway tube could be combined with an intubation tube in clinical applications. In the experiment, a switching transistor controlled high frequency DC (12.5 kHz) was used to create a continuous discharge arc between two stainless steel electrodes (1-mm separation) after high-voltage breakthrough. A rotate instrument was employed to change the direction angle between the airflow and discharge arc, tube filled with Calcium hydroxide connected with gas outlet for reducing NO2, gas flow rate and input voltage were evaluated separately with concentration of NO and NO2/NO ratio. Results showed that a 2 L/min air flow direction from the cathode to the anode of electrodes (direction angle was zero) under 4 V input voltages produced 32.5±3.8 ppm NO, and the NO2/NO ratio reduced to less than 10%, stable output of nitric oxide might be convenient and effective for NO inhalation therapy. Modularization of the design produced a portable NO inhalation device that has potential for use in clinical applications as it is low cost, easy to disinfect, consumes low levels of energy and is ready to use.


Asunto(s)
Tratamiento de Urgencia/instrumentación , Diseño de Equipo , Óxido Nítrico/metabolismo , Gases em Plasma/química , Neumonía Viral/terapia , Terapia Respiratoria/instrumentación , Ventiladores Mecánicos , Administración por Inhalación , Tratamiento de Urgencia/métodos , Humanos , Óxido Nítrico/administración & dosificación , Terapia Respiratoria/métodos
14.
J Vis Exp ; (159)2020 05 25.
Artículo en Inglés | MEDLINE | ID: mdl-32510497

RESUMEN

Electrospinning affords researchers the opportunity to fabricate reproducible micro to nanoscale polymer fibers. The 3D fibrous architecture of electrospun polymers is regarded as a structural imitation of the extracellular matrix (ECM). Hence, electrospun fibers fabricated from biocompatible polymers have been widely investigated by tissue engineering researchers for their potential role as an artificial ECM for guiding tissue growth both in vitro and in vivo. All cells are acutely sensitive to their mechanical environment. This has been demonstrated by the discovery of multiple mechanotransduction pathways intrinsically linked to the cytoskeletal actin filaments. The cytoskeleton acts as a mechanical sensor that can direct the functionality and differentiation of the host cell depending on the stiffness and morphology of its substrate. Electrospun fibers can be tuned both in terms of fiber size and morphology to easily modulate the mechanical environment within a fibrous polymer scaffold. Here, methods for electrospinning polycaprolactone (PCL) for three distinct morphologies at two different fiber diameters are described. The morphological fiber categories consist of randomly oriented fibers, aligned fibers, and porous cryogenically spun fibers, with 1 µm and 5 µm diameters. The methods detailed within this study are proposed as a platform for investigating the effect of electrospun fiber architecture on tissue generation. Understanding these effects will allow researchers to optimize the mechanical properties of electrospun fibers and demonstrate the potential of this technology more thoroughly.


Asunto(s)
Poliésteres/química , Ingeniería de Tejidos/métodos , Diferenciación Celular , Células Hep G2 , Humanos , Gases em Plasma/química , Porosidad , Esterilización , Resistencia a la Tracción , Andamios del Tejido/química
15.
Food Chem ; 328: 127053, 2020 Oct 30.
Artículo en Inglés | MEDLINE | ID: mdl-32464553

RESUMEN

Compared with dried noodles, fresh wet noodles (FWNs) have unique flavor and texture characteristics. However, they have a short shelf life due to their high moisture content (approximately 33%). We developed an innovative technique applying cold plasma, which allows the quick removal of some of the moisture to improve their shelf-life. We investigated the effect of cold plasma treatment on the extent of moisture molecule migration and on quality changes in FWNs. A rapid moisture molecule migration occurred due to the synergism between the structure driving force and drying driving force caused by cold plasma. The color of FWNs became whiter and the texture became harder upon plasma treatment. Scanning electron microscopy and differential scanning calorimetry showed that the structural stability of FWNs increased. Moreover, cold plasma treatment had no effect on the secondary structure types of FWNs, while the α-helix and ß-sheet converted to ß-turn and random coil.


Asunto(s)
Harina/análisis , Gases em Plasma/química , Rastreo Diferencial de Calorimetría , Deshidratación , Microscopía Electrónica de Rastreo , Gusto
16.
J Appl Microbiol ; 129(5): 1248-1260, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32358824

RESUMEN

AIMS: The study aimed to investigate the inactivation efficacy and mechanisms of plasma activated water (PAW) on selected bacteria in planktonic state. METHODS AND RESULTS: Plasma activated water was generated using an atmospheric cold plasma jet at 15, 22 and 30 kV for 5 min. Escherichia coli, Listeria innocua, Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas fluorescens and Shewanella putrefaciens were selected as the representative bacterial species. Each bacterial suspension was inoculated into PAW immediately after generation, and the viable counts at different exposure times of 0·5, 1, 3, 5 and 24 h during 4°C storage were measured to determine the inactivation efficacy. Scanning electron microscopy images of the bacteria were conducted to examine the structural changes. Physicochemical properties of PAW, including pH, conductivity, oxidation reduction potential (ORP), and reactive species of H2 O2 , NO2 - and NO3 - were measured. The results demonstrated that inactivation efficacy was in positive correlation with voltage and exposure time. Gram-negative bacteria were more susceptible to PAW than Gram-positive bacteria. Morphology damage was observed for all the bacterial species. PAW was significantly acidified, conductivity and ORP were significantly increased, and reactive species were detectable after 48 h. CONCLUSIONS: This study offered a better understanding of the inactivation mechanisms of PAW, and the inactivation efficacy can be affected by voltage, exposure time and bacterial species. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrated the potential usage of PAW as an alternative disinfectant.


Asunto(s)
Bacterias/efectos de los fármacos , Desinfectantes/farmacología , Gases em Plasma/química , Agua/farmacología , Bacterias/clasificación , Desinfectantes/química , Radicales Libres/análisis , Radicales Libres/farmacología , Concentración de Iones de Hidrógeno , Viabilidad Microbiana/efectos de los fármacos , Microscopía Electrónica de Rastreo , Especificidad de la Especie , Agua/química , Microbiología del Agua
17.
Artículo en Inglés | MEDLINE | ID: mdl-32432966

RESUMEN

In this study, a newly developed liquid-phase plasma discharge (LPPD) process was evaluated for removing methylene blue (MB) in water. Three independent variables, i.e., liquid flow rate (LF), air flow rate (AF), and MB concentration in water, were all tested at five levels (37, 68, 81, 94, and 103 mL/min for LF; 1, 2, 3, 4, and 5 L/min for AF; and 10, 30, 50, 70, and 90 mg/L for MB) using Central Composite Design (CCD) and Response Surface Methodology (RSM) to optimize the operation for the plasma reactor. The results showed that the regression model produced by the CCD/RSM analysis could adequately predict the MB removal rate of the LPPD process with a model p value of less than 0.0001. The uncertainty analysis further confirmed the error range for the regression model was from -3.93% to 0.63% in predicting the MB removal rate within the ranges of the three independent variables tested. The removal rates were all above *Address correspondence to Dr. Sarah Wu, Department of Biological Engineering, University of Idaho, 875 Perimeter Drive MS 0904, Moscow, ID 83844-0904. 99% for the MB concentrations experimented at LF 68 mL/min and AF 4 L/min. The results concluded that the novel LPPD process was effective in removing MB from dye wastewater.


Asunto(s)
Técnicas Electroquímicas/métodos , Azul de Metileno/análisis , Aguas Residuales/química , Contaminantes Químicos del Agua/análisis , Purificación del Agua/métodos , Adsorción , Modelos Teóricos , Gases em Plasma/química
18.
Trends Biotechnol ; 38(11): 1278-1291, 2020 11.
Artículo en Inglés | MEDLINE | ID: mdl-32418663

RESUMEN

Viruses can infect all cell-based organisms, from bacteria to humans, animals, and plants. They are responsible for numerous cases of hospitalization, many deaths, and widespread crop destruction, all of which result in an enormous medical, economical, and biological burden. Each of the currently used decontamination methods has important drawbacks. Cold plasma (CP) has entered this field as a novel, efficient, and clean solution for virus inactivation. We present recent developments in this promising field of CP-mediated virus inactivation, and describe the applications and mechanisms of the inactivation. This is particularly relevant because viral pandemics, such as COVID-19, highlight the need for alternative virus inactivation methods to replace, complement, or upgrade existing procedures.


Asunto(s)
Betacoronavirus , Infecciones por Coronavirus/prevención & control , Pandemias/prevención & control , Gases em Plasma/farmacología , Neumonía Viral/prevención & control , Inactivación de Virus , Animales , Bacteriófagos/patogenicidad , Betacoronavirus/patogenicidad , Biotecnología/instrumentación , Infecciones por Coronavirus/transmisión , Descontaminación/métodos , Desinfección/métodos , Microbiología Ambiental , Humanos , Modelos Biológicos , Virus de Plantas/patogenicidad , Gases em Plasma/química , Neumonía Viral/transmisión , Prueba de Estudio Conceptual , Virus/patogenicidad
19.
Int J Food Microbiol ; 324: 108612, 2020 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-32244103

RESUMEN

Effect of in-bag dielectric barrier discharge cold plasma (IB-DBD-CP) on the keeping quality of Asian sea bass slices (ASBS) packaged under different gases during refrigerated storage at 4 °C was studied. ASBS without and with IB-DBD-CP treatment packaged under the gas combination of argon and oxygen (10:90) (gas A) or the mixtures of carbon dioxide, argon and oxygen (60:30:10) (gas B) and the control (kept in air) were monitored for quality changes up to 18 days. ASBS treated with IB-DBD-CP, regardless of gas composition, had lower microbial loads than those without treatment and the control (p < 0.05). The shelf-life of ASBS was prolonged to 9 and 12 days after being packaged under gas A and B, respectively without IB-DBD-CP treatment, while 6 days were recorded for the control. However, ASBS treated with IB-DBD-CP, packaged under gas A and B had the shelf-life of 12 and 15 days, respectively. Throughout the storage, trimethylamine content and total volatile nitrogen base content were lower in ASBS treated with IB-DBD-CP, particularly those packaged under gas B than that without treatment and the control (p < 0.05). Nevertheless, lipid oxidation as well as protein oxidation were higher in samples treated with IB-DBD-CP, regardless of gas composition used, in comparison with untreated counterpart. Therefore, IB-DBD-CP of ASBS packaged under high ratio of CO2 (60%) along with argon and oxygen was the potential method for augmenting the shelf-life of ASBS for >15 days at 4 °C.


Asunto(s)
Lubina , Conservación de Alimentos/métodos , Almacenamiento de Alimentos/métodos , Gases em Plasma/química , Alimentos Marinos/análisis , Animales , Argón/análisis , Lubina/microbiología , Dióxido de Carbono/análisis , Calidad de los Alimentos , Oxígeno/análisis , Alimentos Marinos/microbiología
20.
Sci Rep ; 10(1): 3673, 2020 02 28.
Artículo en Inglés | MEDLINE | ID: mdl-32111863

RESUMEN

Fungal diseases seriously affect agricultural production and the food industry. Crop protection is usually achieved by synthetic fungicides, therefore more sustainable and innovative technologies are increasingly required. The atmospheric pressure low-temperature plasma is a novel suitable measure. We report on the effect of plasma treatment on phytopathogenic fungi causing quantitative and qualitative losses of products both in the field and postharvest. We focus our attention on the in vitro direct inhibitory effect of non-contact Surface Dielectric Barrier Discharge on conidia germination of Botrytis cinerea, Monilinia fructicola, Aspergillus carbonarius and Alternaria alternata. A few minutes of treatment was required to completely inactivate the fungi on an artificial medium. Morphological analysis of spores by Scanning Electron Microscopy suggests that the main mechanism is plasma etching due to Reactive Oxygen Species or UV radiation. Spectroscopic analysis of plasma generated in humid air gives the hint that the rotational temperature of gas should not play a relevant role being very close to room temperature. In vivo experiments on artificially inoculated cherry fruits demonstrated that inactivation of fungal spores by the direct inhibitory effect of plasma extend their shelf life. Pre-treatment of fruits before inoculation improve the resistance to infections maybe by activating defense responses in plant tissues.


Asunto(s)
Hongos Mitospóricos/crecimiento & desarrollo , Enfermedades de las Plantas/microbiología , Gases em Plasma , Esporas Fúngicas/crecimiento & desarrollo , Gases em Plasma/química , Gases em Plasma/farmacología
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