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1.
Int J Mol Sci ; 22(5)2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33652991

RESUMEN

A hydrogel system based on oxidized alginate covalently crosslinked with gelatin (ADA-GEL) has been utilized for different biofabrication approaches to design constructs, in which cell growth, proliferation and migration have been observed. However, cell-bioink interactions are not completely understood and the potential effects of free aldehyde groups on the living cells have not been investigated. In this study, alginate, ADA and ADA-GEL were characterized via FTIR and NMR, and their effect on cell viability was investigated. In the tested cell lines, there was a concentration-dependent effect of oxidation degree on cell viability, with the strongest cytotoxicity observed after 72 h of culture. Subsequently, primary human cells, namely fibroblasts and endothelial cells (ECs) were grown in ADA and ADA-GEL hydrogels to investigate the molecular effects of oxidized material. In ADA, an extremely strong ROS generation resulting in a rapid depletion of cellular thiols was observed in ECs, leading to rapid necrotic cell death. In contrast, less pronounced cytotoxic effects of ADA were noted on human fibroblasts. Human fibroblasts had higher cellular thiol content than primary ECs and entered apoptosis under strong oxidative stress. The presence of gelatin in the hydrogel improved the primary cell survival, likely by reducing the oxidative stress via binding to the CHO groups. Consequently, ADA-GEL was better tolerated than ADA alone. Fibroblasts were able to survive the oxidative stress in ADA-GEL and re-entered the proliferative phase. To the best of our knowledge, this is the first report that shows in detail the relationship between oxidative stress-induced intracellular processes and alginate di-aldehyde-based bioinks.


Asunto(s)
Alginatos/química , Materiales Biocompatibles/química , Células Endoteliales/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Gelatina/química , Estrés Oxidativo/efectos de los fármacos , Alginatos/toxicidad , Animales , Materiales Biocompatibles/toxicidad , Línea Celular , Supervivencia Celular/efectos de los fármacos , Células Endoteliales/citología , Fibroblastos/citología , Gelatina/toxicidad , Humanos , Ratones , Células 3T3 NIH , Andamios del Tejido/química
2.
Carbohydr Polym ; 260: 117801, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712149

RESUMEN

Slowly digestible starches have received interest due to their lower increase of postprandial blood glucose and insulin levels and, hence, modification of starches towards slower digestibility has commercial interest. However, chemical characteristics driving enzymatic (digestive) degradation are not fully unraveled. The digestion properties of starches have been linked to their crystalline type, chain length distribution, amylose content or degree of branching, but content and length of relatively long side-chains in amylopectin has not been paid attention to. Therefore, this research focusses on the unique content and length of amylopectin side-chains from conventional and new starch sources (potato, corn, pea, and tulip) correlated to the enzymatic digestion. The rate of hydrolysis was found to be correlated with the crystalline type of starch, as previously suggested, however, the complete hydrolysis of all starches, independent of the crystalline type and source, was shown to be governed by the content of longer amylopectin chains.


Asunto(s)
Gelatina/química , Glucano 1,4-alfa-Glucosidasa/metabolismo , Almidón/metabolismo , alfa-Amilasas/metabolismo , Cristalización , Digestión , Hidrólisis , Espectroscopía de Resonancia Magnética , Guisantes/metabolismo , Solanum tuberosum/metabolismo , Almidón/química , Zea mays/metabolismo
3.
Molecules ; 26(4)2021 Feb 19.
Artículo en Inglés | MEDLINE | ID: mdl-33669650

RESUMEN

Apples are an important source of biologically active compounds. Consequently, we decided to model hard gelatin capsules with lyophilized apple powder by using different excipients and to evaluate the release kinetics of phenolic compounds. The apple slices of "Ligol" cultivar were immediately frozen in a freezer (at -35°C) with air circulation and were lyophilized with a sublimator at the pressure of 0.01 mbar (condenser temperature, -85°C). Lyophilized apple powder was used as an active substance filled into hard gelatin capsules. We conducted capsule disintegration and dissolution tests to evaluate the quality of apple lyophilizate-containing capsules of different encapsulating content. Individual phenolic compounds can be arranged in the following descending order according to the amount released from the capsules of different compositions: chlorogenic acid > rutin > avicularin > hyperoside > phloridzin > quercitrin > (-)-epicatechin > isoquercitrin. Chlorogenic acid was the compound that was released in the highest amounts from capsules of different encapsulating content: its released amounts ranged from 68.4 to 640.3 µg/mL. According to the obtained data, when hypromellose content ranged from 29% to 41% of the capsule mass, the capsules disintegrated within less than 30 min, and such amounts of hypromellose did not prolong the release of phenolic compounds. Based on the results of the dissolution test, the capsules can be classified as fast-dissolving preparations, as more than 85% of the active substances were released within 30 min.


Asunto(s)
Productos Biológicos/análisis , Cápsulas/química , Liofilización , Malus/química , Etanol/química , Gelatina/química , Glicósidos/química , Fenoles/análisis , Fenoles/química , Polvos
4.
Int J Mol Sci ; 22(4)2021 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-33669209

RESUMEN

Nisin Z, an amphipathic peptide, with a significant antibacterial activity against Gram-positive bacteria and low toxicity in humans, has been studied for food preservation applications. Thus far, very little research has been done to explore its potential in biomedicine. Here, we report the modification of sodium alginate (SA) and gelatin (GN) blended microfibers, produced via the wet-spinning technique, with Nisin Z, with the purpose of eradicating Staphylococcus aureus-induced infections. Wet-spun SAGN microfibers were successfully produced at a 70/30% v/v of SA (2 wt%)/GN (1 wt%) polymer ratio by extrusion within a calcium chloride (CaCl2) coagulation bath. Modifications to the biodegradable fibers' chemical stability and structure were then introduced via crosslinking with CaCl2 and glutaraldehyde (SAGNCL). Regardless of the chemical modification employed, all microfibers were labelled as homogeneous both in size (≈246.79 µm) and shape (cylindrical and defect-free). SA-free microfibers, with an increased surface area for peptide immobilization, originated from the action of phosphate buffer saline solution on SAGN fibers, were also produced (GNCL). Their durability in physiological conditions (simulated body fluid) was, however, compromised very early in the experiment (day 1 and 3, with and without Nisin Z, respectively). Only the crosslinked SAGNCL fibers remained intact for the 28 day-testing period. Their thermal resilience in comparison with the unmodified and SA-free fibers was also demonstrated. Nisin Z was functionalized onto the unmodified and chemically altered fibers at an average concentration of 178 µg/mL. Nisin Z did not impact on the fiber's morphology nor on their chemical/thermal stability. However, the peptide improved the SA fibers (control) structural integrity, guaranteeing its stability for longer, in physiological conditions. Its main effect was detected on the time-kill kinetics of the bacteria S. aureus. SAGNCL and GNCL loaded with Nisin Z were capable of progressively eliminating the bacteria, reaching an inhibition superior to 99% after 24 h of culture. The peptide-modified SA and SAGN were not as effective, losing their antimicrobial action after 6 h of incubation. Bacteria elimination was consistent with the release kinetics of Nisin Z from the fibers. In general, data revealed the increased potential and durable effect of Nisin Z (significantly superior to its free, unloaded form) against S. aureus-induced infections, while loaded onto prospective biomedical wet-spun scaffolds.


Asunto(s)
Alginatos/química , Antibacterianos/química , Antibacterianos/farmacología , Reactivos de Enlaces Cruzados/química , Gelatina/química , Nisina/análogos & derivados , Staphylococcus aureus/efectos de los fármacos , Materiales Biocompatibles/química , Plásticos Biodegradables/química , Biopolímeros/química , Cloruro de Calcio/química , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos , Glutaral/química , Cinética , Pruebas de Sensibilidad Microbiana , Nisina/química , Nisina/farmacología , Porosidad , Solubilidad , Infecciones Estafilocócicas/tratamiento farmacológico , Infecciones Estafilocócicas/microbiología , Agua/química
5.
Food Chem ; 351: 129347, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33647702

RESUMEN

In this study, two active packaging types were produced: single-layer biopolymer films with a polysaccharide - furcellaran and carp skin gelatin hydrolysate; two-layer films with identical composition, but synthetic peptide Alanina-Tyrosine addition. The procedure objective was multiplying antioxidant effects of the hydrolysate complexed with furcellaran. Films were used on Atlantic mackerel (storage 4 °C, 15 days); samples were analysed for changes in microbiological quality, TVB-N, biogenic amine content, fatty acid composition, TBARS. Consumer analysis was performed characterising mackerel carcass perception depending on implemented active coatings. The developed innovative single- and double-layer coatings effectively slow down lipid oxidation processes, especially at the initial period of Atlantic mackerel storage in refrigerated conditions. The coatings effectively inhibited microorganism growth, extending shelf-life by 2 days, single-layer coatings showing greater efficiency. According to consumers, coating application did not adversely affect product attractiveness parameters. The developed innovative coatings show great applicative potential as a new active packaging for perishable foods.


Asunto(s)
Alginatos/química , Antioxidantes/química , Carpas/anatomía & histología , Embalaje de Alimentos/métodos , Gelatina/química , Péptidos/química , Gomas de Plantas/química , Piel/química , Animales , Hidrólisis , Oxidación-Reducción , Perciformes , Alimentos Marinos/análisis
6.
Food Chem ; 345: 128865, 2021 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-33601664

RESUMEN

To enhance the physicochemical stability of ω-3 PUFAs concentrates from fish oil, biopolymer coating based on chitosan (CH) and gelatin (GE) deposited on the surface of nanoliposomes (NLs) has been synthesized and characterized. The mean particle size of surface-decorated nanoliposomes (SDNLs) containing ω-3 PUFAs concentrates was found to be in the range of 209.5-454.3 nm. Scanning and transmission electron microscopy revealed the spherical shape and smooth surface of the nanovesicles. Fourier-transform infrared spectroscopy and X-ray diffraction observations confirmed that the NLs have been successfully coated by biopolymeric blends. The highest entrapment efficiency of 81.6% was obtained in polymer-stabilized NLs with a concentration ratio of 0.3:0.1 (CH:GE). Differential scanning calorimetry results revealed enhanced thermal stability of vesicles after polymeric blend desorption. Finally, the oxidative stability assays demonstrated that the ω-3 PUFAs concentrates entrapped in SDNLs was protected against oxidation in comparison to the free ω-3 PUFAs concentrates.


Asunto(s)
Quitosano/química , Ácidos Grasos Omega-3/química , Gelatina/química , Liposomas/química , Nanoestructuras/química , Cromatografía de Gases , Estabilidad de Medicamentos , Ácidos Grasos Omega-3/análisis , Aceites de Pescado/química , Oxidación-Reducción , Tamaño de la Partícula , Temperatura de Transición , Urea/química
7.
Methods Mol Biol ; 2273: 263-278, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33604860

RESUMEN

Tissue engineering is an elegant tool to create organs in vitro, that can help obviate the lack of organ donors in transplantation medicine and provide the opportunity of studying complex biological systems in vitro, thereby reducing the need for animal experiments. Artificial intestine models are at the core of Fish-AI, an EU FET-Open research project dedicated to the development of a 3D in vitro platform that is intended to enable the aquaculture feed industry to predict the nutritional and health value of alternative feed sources accurately and efficiently.At present, it is impossible to infer the health and nutrition value through the chemical characterization of any given feed. Therefore, each new feed must be tested through in vivo growth trials. The procedure is lengthy, expensive and requires the use of many animals. Furthermore, although this process allows for a precise evaluation of the final effect of each feed, it does not improve our basic knowledge of the cellular and molecular mechanisms determining such end-results. In turn, this lack of mechanistic knowledge severely limits the capacity to understand and predict the biological value of a single raw material and of their different combinations.The protocol described herein allows to develop the two main components essential to produce a functional platform for the efficient and reliable screening of feeds that the feed industry is currently developing for improving their health and nutritional value. It is here applied to the Rainbow Trout, but it can be fruitfully used to many other fish species.


Asunto(s)
Técnicas de Cultivo de Célula/métodos , Gelatina/química , Mucosa Intestinal/citología , Oncorhynchus mykiss , Andamios del Tejido/química , Acrilamidas/química , Alimentación Animal/análisis , Animales , Acuicultura/métodos , Materiales Biocompatibles/química , Separación Celular/métodos , Células Cultivadas , Norbornanos/química , Oncorhynchus mykiss/crecimiento & desarrollo
8.
Food Chem ; 349: 129159, 2021 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-33545604

RESUMEN

The effect of dry heat (DH, 130 °C, 1, 3 and 9 h), cold plasma (CP, 40 V, 1, 5 and 10 min) and their combination (D-P) treatment on the structure, physicochemical and digestive properties of red adzuki bean starch were studied. The results showed that DH or CP had slight change in morphology while diffraction pattern of starch was remained. With the extension of treated time of DH and CP, the amylose content, crystallinity, molecular weight, short-range order, the long chain of amylopectin, enthalpy value, swelling power, digestibility were reduced, while gelatinization temperatures, the short chain of amylopectin and solubility were increased. The D-P had deeper modification than the single treatment. The combination of dry heat and cold plasma is a simple and green method to improve the starch structure and enhance starch properties and this modified starch could be implemented to tailor starch to the desired food applications.


Asunto(s)
Frío , Digestión , Calor , Gases em Plasma/química , Almidón/química , Almidón/metabolismo , Vigna/química , Manipulación de Alimentos , Gelatina/química , Peso Molecular , Solubilidad , Termodinámica
9.
Food Chem ; 351: 129226, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33639430

RESUMEN

The effect of three drying methods (hot air, freeze and spray drying) on the solubility and amphiphilicity of gelatin were investigated and compared. Results showed spray drying gelatin (SDG) and hot air drying gelatin (HDG) showed the lowest and best solubility, respectively. This phenomenon was attributed to the degree of subunits degradation and hydrophobicity. The HDG showed an obvious degradation during the hot air drying and displayed the strongest hydrophilicity, while SDG showed a slight degradation and strongest hydrophobicity. The results of wettability showed that SDG had a better amphiphilicity (92.48°) in comparison with HDG (57.7°) and freeze drying gelatin (VDG, 77.53°), which can effectively reduce the interfacial tension of gelatin, thus significantly improving the stability of foam and emulsion (p < 0.05). These results suggested the drying methods can adjust the amphiphilicity of gelatin, and the SDG displayed a better amphiphilicity, showing good potential applications in foam and emulsion.


Asunto(s)
Desecación/métodos , Congelación , Gelatina/química , Gelatina/aislamiento & purificación , Interacciones Hidrofóbicas e Hidrofílicas , Microondas , Solubilidad , Factores de Tiempo , Humectabilidad
10.
Food Chem ; 351: 129269, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33640772

RESUMEN

Chemical modifications of gelatin from mechanically separated chicken meat (MSM) residue were practiced using caffeic acid as a cross-linker. The effects of oxidation period (OP), cross-linking temperature (CT), and caffeic acid (CA) concentration were investigated. Experiments were performed using Response Surface Methodology (RSM). The effects of 16 different cross-linking modifications on the physicochemical properties of chicken gelatin gels were investigated. Maximum gel strength was determined at 12.5 min OP, 50 °C CT and 2.5% CA concentration and this was 63% higher than the control (uncross-linked chicken gelatin). Temperature has an increasing effect on the degree of cross-linking value up to a certain degree. The highest degree of cross-linking was observed at between 50° and 55 °C. The color characteristics of gels were affected by cross-linking having more brown color. Overall this study demonstrated that caffeic acid has a potential to be an efficient natural cross-linking factor increasing the mechanical properties of chicken gelatin thermo-irreversibly.


Asunto(s)
Ácidos Cafeicos/química , Gelatina/química , Animales , Pollos , Oxidación-Reducción , Temperatura
11.
Food Funct ; 12(5): 1983-1995, 2021 Mar 07.
Artículo en Inglés | MEDLINE | ID: mdl-33537688

RESUMEN

To evaluate the effect of young apple polyphenols (YAP) on starch digestion and gut microbiota, complexes of native wheat starch (NWS) with YAP, and their main components chlorogenic acid (CA) and phlorizin (P) were fabricated and gelatinized. Through XRD and FTIR analysis, it was found that the partial crystalline structure of NWS was destroyed during gelatinization, and the addition of P decreased the extent of destruction. Then, the gelatinized starchy samples were subjected to in vitro digestion. The wheat starch (WS)-phenolic compound complexes significantly suppressed the digestion rate and increased the proportion of resistant starch (RS) in WS. Furthermore, the residual starchy components after digestion were fermented by human fecal samples for 24 h. The WS-YAP complex greatly increased the concentration of short-chain fatty acids (SCFAs), especially acetic and propionic acids, and enhanced the growth of health-promoting gut microbiota such as Prevotella. Conclusively, YAP was shown to play a positive role in maintaining blood glucose balance and intestinal health.


Asunto(s)
Digestión/efectos de los fármacos , Fermentación/efectos de los fármacos , Malus/química , Polifenoles/farmacología , Almidón/metabolismo , Triticum/química , Adulto , Bacterias/clasificación , Bacterias/metabolismo , Cristalización , Ácidos Grasos/metabolismo , Ácidos Grasos Volátiles/metabolismo , Heces/microbiología , Frutas/química , Gelatina/química , Humanos , Almidón/química , alfa-Amilasas/metabolismo
12.
Carbohydr Polym ; 255: 117484, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33436244

RESUMEN

Wound dressing composed of chitosan, based crosslinked gelatin/ polyvinyl pyrrolidone, embedded silver nanoparticles were fabricated using solution casting method. The membrane was characterized by FTIR, SEM and TGA. Glutaraldehyde (0.5 %) was used for the crosslinking of membrane components and associated with 7-folds boosted mechanical performance, 28 % more hydrolytic stability, 3-folds thickness reduction and morphological roughness. Silver nanoparticles were characterized by UV-vis, XRD and TEM for an average size of 9.9 nm. The membrane with higher concentration of silver nanoparticles showed maximum antibacterial activity against human pathogenic bacteria; and the measured inhibition zones ranged from 1.5 to 3 cm. The activity of the particles ranged from severe to complete reduction in Penicillin, Erythromycin and Macrolide family's resistance genes expression such as ß-Lactamase, mecA and erm. This developed membrane can serve as promising and cost-effective system against severe diabetic and burn wound infections.


Asunto(s)
Antibacterianos/farmacología , Vendajes , Quitosano/química , Citrullus colocynthis/química , Gelatina/química , Povidona/química , Plata/farmacología , Bacillus subtilis/efectos de los fármacos , Bacillus subtilis/crecimiento & desarrollo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Farmacorresistencia Bacteriana Múltiple/efectos de los fármacos , Farmacorresistencia Bacteriana Múltiple/genética , Eritromicina/farmacología , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Humanos , Macrólidos/farmacología , Nanopartículas del Metal/química , Nanopartículas del Metal/ultraestructura , Metiltransferasas/genética , Metiltransferasas/metabolismo , Pruebas de Sensibilidad Microbiana , Proteínas de Unión a las Penicilinas/genética , Proteínas de Unión a las Penicilinas/metabolismo , Penicilinas/farmacología , Cultivo Primario de Células , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/crecimiento & desarrollo , Salmonella typhi/efectos de los fármacos , Salmonella typhi/crecimiento & desarrollo , Plata/química , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , beta-Lactamasas/genética , beta-Lactamasas/metabolismo
13.
ACS Appl Mater Interfaces ; 13(1): 112-122, 2021 Jan 13.
Artículo en Inglés | MEDLINE | ID: mdl-33397079

RESUMEN

The gold standard treatment for peripheral nerve injuries (PNIs) is the autologous graft, while it is associated with the shortage of donors and results in major complications. In the present study, we engineer a graphene mesh-supported double-network (DN) hydrogel scaffold, loaded with netrin-1. Natural alginate and gelatin-methacryloyl entangled hydrogel that is synthesized via fast exchange of ions and ultraviolet irradiation provide proper mechanical strength and excellent biocompatibility and can also serve as a reservoir for netrin-1. Meanwhile, the graphene mesh can promote the proliferation of Schwann cells and guide their alignments. This approach allows scaffolds to have an acceptable Young's modulus of 725.8 ± 46.52 kPa, matching with peripheral nerves, as well as a satisfactory electrical conductivity of 6.8 ± 0.85 S/m. In addition, netrin-1 plays a dual role in directing axon pathfinding and neuronal migration that optimizes the tube formation ability at a concentration of 100 ng/mL. This netrin-1-loaded graphene mesh tube/DN hydrogel nerve scaffold can significantly promote the regeneration of peripheral nerves and the restoration of denervated muscle, which is even superior to autologous grafts. Our findings may provide an effective therapeutic strategy for PNI patients that can replace the scarce autologous graft.


Asunto(s)
Grafito/química , Hidrogeles/química , Regeneración Nerviosa/efectos de los fármacos , Netrina-1/uso terapéutico , Traumatismos de los Nervios Periféricos/tratamiento farmacológico , Nervio Ciático/efectos de los fármacos , Alginatos/química , Alginatos/toxicidad , Animales , Movimiento Celular/efectos de los fármacos , Sistemas de Liberación de Medicamentos , Módulo de Elasticidad , Gelatina/química , Gelatina/toxicidad , Grafito/toxicidad , Células Endoteliales de la Vena Umbilical Humana , Humanos , Hidrogeles/toxicidad , Masculino , Metacrilatos/química , Metacrilatos/toxicidad , Neovascularización Fisiológica/efectos de los fármacos , Ratas Sprague-Dawley , Células de Schwann/efectos de los fármacos , Nervio Ciático/lesiones , Andamios del Tejido/química
14.
Food Chem ; 348: 129055, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-33508595

RESUMEN

The study evaluated the potential and antioxidant stability of nanoencapsulated carotenoid-rich extract (CE) from Cantaloupe melon (EPG). DPPH and ABTS radical scavenging assays were used to investigate the nanoencapsulation effect on antioxidant potential. CE and EPG stability were evaluated at 25 °C and 5 °C, with and without light (1600 lx) for 60 days, determining the ß-carotene concentration by UHPLC and antioxidant potential by ABTS. The antioxidant potential of carotenoids increased after nanoencapsulation (57-59%). After 60 days, there was low retention of ß-carotene (0-43.6%) in the CE, mainly at 25 °C light (0.00%) and dark (10.0%), and total loss of activity in the four conditions. EPG preserved the ß-carotene concentration in the dark at 25 °C (99.0%) and in the light (83.1%) and dark (99.0%) at 5 °C, maintaining the antioxidant potential (68.7-48.3%). Therefore, EPG enhanced and stabilized the antioxidant potential of carotenoids, beneficial to human health.


Asunto(s)
Antioxidantes/química , Antioxidantes/aislamiento & purificación , Carotenoides/análisis , Cucumis melo/química , Almacenamiento de Alimentos , Gelatina/química , Nanoestructuras/química , Cápsulas , Frutas/química , Humanos
15.
Int J Biol Macromol ; 172: 580-588, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33476616

RESUMEN

Tissue adhesives have been developed for sealing tissue damaged in surgery. Among these, sheet-type adhesives require a relatively long time to adhere to biological tissue under wet conditions. To address this clinical problem, we fabricated a tissue-adhesive fiber sheet (AdFS) based on decanyl group (C10) modified Alaska pollock-derived gelatin (C10-ApGltn) using electrospinning. Ultraviolet (UV) irradiation of the AdFS was performed to increase the affinity between the AdFS and wet biological tissue by introducing hydrophilic functional groups. The UV irradiated AdFS (UV-C10-AdFS) strongly adhered to porcine pleura within 2 min under wet conditions and showed higher burst strength compared with the original ApGltn (Org-ApGltn) sheet. Hematoxylin-eosin stained sections revealed that a dense UV-C10-AdFS layer remained on the surface of the porcine pleura even after burst strength measurement. Moreover, UV-C10-AdFS has excellent cytocompatibility and efficiently supports the growth of L929 cells. UV-C10-AdFS is a promising adhesive material for sealing wet biological tissue.


Asunto(s)
Gelatina/química , Pleura/efectos de los fármacos , Adhesivos Tisulares/química , Adhesivos Tisulares/efectos de la radiación , Alaska , Animales , Línea Celular , Técnicas Electroquímicas , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Gadiformes/metabolismo , Gelatina/efectos de la radiación , Interacciones Hidrofóbicas e Hidrofílicas , Ensayo de Materiales , Ratones , Pleura/efectos de la radiación , Polietilenglicoles/química , Porcinos , Resistencia a la Tracción , Adhesivos Tisulares/farmacología , Rayos Ultravioleta
16.
Int J Biol Macromol ; 173: 203-210, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33484799

RESUMEN

Nonwoven fabrics containing silver nanoparticles (AgNPs) are widely utilized to assist management of infected wounds and those at risk of infection. However, such materials have varied responses due to their chemical nature. Herein we investigated the correlation between the concentration of AgNPs taken up by nonwoven viscose material and antibacterial activity in a simulated wound fluid model against two bacterial models (i.e., Escherichia coli and Staphylococcus aureus). Thereafter, the developed nonwoven viscose containing AgNPs were independently coated with two polyacid carbohydrate polymers (i.e., carboxymethyl chitosan (CMCs), alginate (ALG)), and gelatin (GEL) protein in order to study their influence on the physical and biological attributes in vitro and in vivo. Intensive characterizations were utilized to monitor the physicochemical features of the developed nonwoven viscose. The results demonstrated that higher concentrations of AgNPs were taken up by viscose fabric whilewhile increasing AgNPs in the colloidal solution during padding process. Overall, the treated nonwoven fabric with and without polymers' coatings showed remarkable antibacterial activity against two bacterial models in vitro. As well as they achieved high and speed wound recovery in rats which was almost similar to commercial dermazin treatment. Therefore, it validates excellent nonwoven dressing clinically relevant to the wound type and condition.


Asunto(s)
Quemaduras Químicas/tratamiento farmacológico , Infecciones por Escherichia coli/tratamiento farmacológico , Nanopartículas del Metal/química , Plata/farmacología , Infecciones Estafilocócicas/tratamiento farmacológico , Cicatrización de Heridas/efectos de los fármacos , Alginatos/química , Animales , Antibacterianos/química , Antibacterianos/farmacología , Vendajes , Quemaduras Químicas/microbiología , Carboximetilcelulosa de Sodio/química , Quitosano/análogos & derivados , Quitosano/química , Preparaciones de Acción Retardada/química , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Infecciones por Escherichia coli/microbiología , Gelatina/química , Nanopartículas del Metal/ultraestructura , Ratas , Plata/química , Piel/efectos de los fármacos , Piel/microbiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo , Cicatrización de Heridas/fisiología
17.
Int J Biol Macromol ; 171: 1-9, 2021 Feb 28.
Artículo en Inglés | MEDLINE | ID: mdl-33412193

RESUMEN

In this study, the microwave irradiation as a green approach was applied to improve the properties (mainly solubility and interface properties) of pigskin gelatin. The results showed that the solubility of pigskin gelatin was improved obviously at room temperature (25 °C) due to the destruction of polymer subunits. Furthermore, the exposure of more hydrophobic groups in microwave-irradiated gelatin increased its hydrophobicity, consequently improving the amphiphilic property and the interfacial properties of gelatin. The results of interface behavior showed that the interfacial tension of microwave-irradiated gelatin was reduced obviously with the extension of irradiation time (0-30 min), which is more beneficial to adsorption of gelatin molecules at the interface, thus resulting in a significant increase of adsorption rate (AP) from 56.13% (0 min) to 91.87% (30 min). Correspondingly, the foaming and emulsifying properties of gelatin were also improved significantly (p < 0.05). This study would promote the development of food-grade foam and emulsion based on pigskin gelatin by adjusting solubility and interface properties.


Asunto(s)
Gelatina/química , Tecnología Química Verde , Adsorción , Animales , Cosméticos/química , Emulsiones , Tecnología de Alimentos/métodos , Gelatina/aislamiento & purificación , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Microondas , Piel/química , Solubilidad , Porcinos , Temperatura , Factores de Tiempo
18.
Carbohydr Polym ; 256: 117520, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33483041

RESUMEN

Xylan and gelatin-based hydrogels are prepared in different molar ratios using ethylene glycol diglycidyl ether cross-linker. The hydrogels are characterized by Fourier transform infrared spectroscopy, morphology, thermal analysis, and swelling ratio. The rheological experiment shows the gels are cross-linked successfully by revealing the viscoelastic nature. The xylan-gelatin gel synthesized in a 5:1 molar ratio (hence XG5) has higher storage modulus, gelation temperature, and time among the synthesized gels. The adsorption behaviour of the synthesized gels is studied for the removal of methylene blue, by varying adsorbate concentration, pH, and temperature. Among the synthesized hydrogels, XG5 shows the highest adsorption capacity of 26.04 mg g-1 at pH = 5.84 and 25 °C. The kinetics of the adsorption process follows the pseudo-second-order model, and monolayer adsorption is adequately represented by the Langmuir isotherm model. The adsorption process is established as spontaneous, exothermic, and physisorption from the thermodynamic parameters.


Asunto(s)
Gelatina/química , Hidrogeles/química , Azul de Metileno/aislamiento & purificación , Contaminantes Químicos del Agua/aislamiento & purificación , Xilanos/química , Adsorción , Reactivos de Enlaces Cruzados/química , Elasticidad , Resinas Epoxi/química , Concentración de Iones de Hidrógeno , Cinética , Azul de Metileno/química , Reología , Temperatura , Termodinámica , Viscosidad , Contaminantes Químicos del Agua/química , Humectabilidad
19.
Food Chem ; 348: 129093, 2021 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-33503534

RESUMEN

Fish gelatin (FG) has been extensively studied as a potential substitute for mammal gelatin. However, FG often requires different modification methods to change its physical and chemical properties due to its low gelling properties. Here, γ-polyglutamic acid (γ-PGA) and microbial transglutaminase (MTGase) were combined to modify FG to improve its gelling properties. The γ-PGA at 0.04% (w/v) and MTGase of different concentrations (0.02-0.08%, w/v) were used to modify FG, and the effects of complex modification on the gelling properties and structure of FG were studied. When the MTGase content was 0.08% (w/v), FG had the best gelling properties. In addition, the complex modification of MTGase and γ-PGA hindered the formation of the triple helix during the FG gel process. This reduced the gel rate, but significantly increased its viscosity. A schematic model was also proposed to illustrate the complex modifications of FG by MTGase and γ-PGA.


Asunto(s)
Peces , Gelatina/química , Ácido Poliglutámico/análogos & derivados , Reología , Transglutaminasas/química , Animales , Geles , Ácido Poliglutámico/química , Viscosidad
20.
Int J Biol Macromol ; 169: 414-427, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-33352152

RESUMEN

The metamorphosis of biodegradable polymers in biomedical applications is an auspicious myriad of indagation. The utmost challenge in clinical conditions includes trauma, organs failure, soft and hard tissues, infection, cancer and inflammation, congenital disorders which are still not medicated efficiently. To overcome this bone of contention, proliferation in the concatenation of biodegradable materials for clinical applications has emerged as a silver bullet owing to eco-friendly, nontoxicity, exorbitant mechanical properties, cost efficiency, and degradability. Several bioimplants are designed and fabricated in a way to reabsorb or degrade inside the body after performing the specific function rather than eliminating the bioimplants. The objective of this comprehensive is to unfurl the anecdote of emerging biological polymers derived implants including silk, lignin, soy, collagen, gelatin, chitosan, alginate, starch, etc. by explicating the selection, fabrication, properties, and applications. Into the bargain, emphasis on the significant characteristics of current discernment and purview of nanotechnology integrated biopolymeric implants has also been expounded. This robust contrivance shed light on recent inclinations and evolution in tissue regeneration and targeting organs followed by precedency and fly in the ointment concerning biodegradable implants evolved by employing fringe benefits provided by 3D printing technology for building tissues or organs construct for implantation.


Asunto(s)
Implantes Absorbibles/tendencias , Materiales Biocompatibles/química , Animales , Quitosano/química , Colágeno/metabolismo , Gelatina/química , Humanos , Nanotecnología/métodos , Polímeros/química , Impresión Tridimensional/tendencias , Ingeniería de Tejidos/métodos , Andamios del Tejido/química
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