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1.
Zhonghua Zhong Liu Za Zhi ; 42(2): 122-126, 2020 Feb 23.
Artículo en Chino | MEDLINE | ID: mdl-32135646

RESUMEN

Objective: To investigate the expression of type Ⅰ collagen α1 chain protein (COL1A1) in triple negative breast cancer (TNBC), and its relationship with clinicopathological features and prognosis of TNBC. Methods: A total of 148 TNBC specimens were collected from the Affiliated Cancer Hospital of Xinjiang Medical University from 2013 to 2015. The mRNA expression of COL1A1 was detected by fluorescence quantitative RT-PCR and the protein expression of COL1A1 was detected by Western blot. The expression of COL1A1 and α-smooth muscle actin (α-SMA) in TNBC were detected by immunohistochemistry. The relationship between the expression of COL1A1 and clinicopathological parameters and prognosis of TNBC patients was analyzed. Results: The mRNA and protein expression of COL1A1 in MDA-MB-231 cells were 1.696±0.486 and 0.550±0.088, respectively, which were higher than those in MCF-10A cells (1.020±0.231 and 0.350±0.083, P=0.032, P=0.046). The mRNA and protein expression of COL1A1 in TNBC tissues were 1.632 ±0.598 and 0.733 ±0.068, respectively, which were higher than those in paracancerous tissues (1.041±0.316 and 0.612±0.016, P=0.003, P=0.039). The high expression rates of COL1A1 and α-SMA in TNBC tissues were 35.8% and 56.7% respectively, which were higher than those in paracancerous tissues (16.7% and 30.0%, P=0.041, P=0.037). The expression of COL1A1 was correlated with tumor size, TNM stage, lymph node metastasis, vascular invasion and α-SMA expression (all P<0.05). The median survival time in COL1A1 high expression group was 64 months, which was lower than that in low expression group (73 months, P<0.05). Multivariate analysis of Cox proportional hazard regression model showed that COL1A1 expression was an independent influencing factor for the survival of TNBC patients (HR=3.952, P=0.004). Conclusion: The high expression of COL1A1 in TNBC is an independent prognostic factor of TNBC patients.


Asunto(s)
Colágeno Tipo I/biosíntesis , Neoplasias de la Mama Triple Negativas/metabolismo , Actinas/biosíntesis , Humanos , Inmunohistoquímica , Metástasis Linfática , Pronóstico , Biosíntesis de Proteínas , ARN Mensajero/biosíntesis , Neoplasias de la Mama Triple Negativas/patología
2.
Rev Soc Bras Med Trop ; 53: e20190446, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32130324

RESUMEN

INTRODUCTION: Visceral leishmaniasis (VL) represents a public health concern in several areas of the world. In the American continent, VL transmission is typically zoonotic, but humans with active VL caused by Leishmania infantum are able to infect sandflies. Thus, individuals with cutaneous parasitic infections may act as reservoirs and allow interhuman transmission. Additionally, the skin may be responsible for reactivation of the disease after therapy. This study's objective was to evaluate cutaneous parasitism in humans with VL in an American endemic area. METHODS: A cross-sectional hospital-based study was conducted in northeast Brazil from October 2016 to April 2017. Biopsies of healthy skin for histopathology and immunohistochemistry were performed prior to treatment in all study patients. RESULTS: Twenty-two patients between the ages of five months to 78 years were included in the study. Seven patients (31.8%) tested positive for HIV. Only one patient had cutaneous parasitism, as confirmed by immunohistochemistry prior to treatment. Parasitism was not detected after treatment. CONCLUSIONS: Cutaneous parasitism in the healthy skin of humans with visceral leishmaniasis, although unusual, may be a source of infection for phlebotomine sandflies.


Asunto(s)
Leishmania infantum/aislamiento & purificación , Leishmaniasis Visceral/parasitología , Piel/parasitología , Adolescente , Adulto , Anciano , Biopsia , Niño , Preescolar , Estudios Transversales , Escolaridad , Enfermedades Endémicas , Femenino , Humanos , Inmunohistoquímica , Lactante , Leishmaniasis Visceral/patología , Masculino , Persona de Mediana Edad , Piel/patología , Adulto Joven
3.
Cancer Treat Rev ; 85: 101995, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32113080

RESUMEN

Up to one in four patients with nasopharyngeal carcinoma present with non-metastatic stage IV disease (i.e. T4 or N3). Distinct failure patterns exist, despite the routine adoption of contemporary treatment modalities such as intensity modulated radiotherapy and systemic chemotherapy. Concurrent chemoradiotherapy (CCRT) followed by adjuvant chemotherapy or induction chemotherapy followed by CCRT are commonly employed in this setting, with the latter emerging as the preferred option. Additionally, emerging radiation technologies like proton therapy has become available offering new opportunities for prevention of radiation-induced side effects. This article reviews not only the current treatment strategies, but also discusses novel ways to tackle this challenging disease with respect to the patterns of failure.


Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Causas de Muerte , Carcinoma Nasofaríngeo/patología , Carcinoma Nasofaríngeo/terapia , Neoplasias Nasofaríngeas/patología , Neoplasias Nasofaríngeas/terapia , Biopsia con Aguja , Quimioradioterapia/métodos , Terapia Combinada , Supervivencia sin Enfermedad , Femenino , Humanos , Inmunohistoquímica , Quimioterapia de Inducción/métodos , Masculino , Carcinoma Nasofaríngeo/diagnóstico por imagen , Carcinoma Nasofaríngeo/mortalidad , Neoplasias Nasofaríngeas/diagnóstico por imagen , Neoplasias Nasofaríngeas/mortalidad , Invasividad Neoplásica/patología , Metástasis de la Neoplasia , Estadificación de Neoplasias , Tomografía de Emisión de Positrones/métodos , Pronóstico , Medición de Riesgo , Análisis de Supervivencia , Resultado del Tratamiento
4.
Acta Cir Bras ; 34(12): e201901202, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32049183

RESUMEN

PURPOSE: To explore the potential role and unclear molecular mechanisms of vaccarin in wound healing. METHODS: Rats' skin excision model to study the effects of vaccarin on wound healing in vivo . Hematoxylin and eosin staining was performed to evaluate Histopathologic characteristics. Immunohistochemistry was employed to assess the effects of vaccarin in accelerating angiogenesis. Western blot was used to evaluate relative protein expressed levels. RESULTS: Vaccarin could significantly promote wound healing and endothelial cells and fibroblasts proliferation in the wound site. Immunohistochemistry and Western blot studies showed that the nodal proteins and receptor (bFGFR) related to angiogenesis signaling pathway were activated, and the microvascular density in the wound site was markedly higher than that in the control group. CONCLUSIONS: The present study was the first to demonstrate that vaccarin is able to induce angiogenesis and accelerate wound healing in vivo by increasing expressions of p-Akt, p-Erk and p-bFGFR. This process is mediated by MAPK/ERK and PI3K/AKT signaling pathways.


Asunto(s)
Inductores de la Angiogénesis/farmacología , Caryophyllaceae/química , Quinasas de Proteína Quinasa Activadas por Mitógenos/efectos de los fármacos , Fosfatidilinositol 3-Quinasas/efectos de los fármacos , Extractos Vegetales/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Western Blotting , Proliferación Celular/efectos de los fármacos , Células Endoteliales/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Inmunohistoquímica , Masculino , Quinasas de Proteína Quinasa Activadas por Mitógenos/análisis , Fosfatidilinositol 3-Quinasas/análisis , Extractos Vegetales/química , Ratas Sprague-Dawley , Receptor Tipo 1 de Factor de Crecimiento de Fibroblastos/análisis , Receptor Tipo 1 de Factor de Crecimiento de Fibroblastos/efectos de los fármacos , Reproducibilidad de los Resultados , Transducción de Señal , Factores de Tiempo
5.
Acta Cir Bras ; 34(12): e201901204, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32074166

RESUMEN

PURPOSE: To examine the therapeutic effect of external adenosine on an acetic acid-induced acute ulcerative colitis model in rats. METHODS: Thirty male mature rats were divided into three groups as control, acute colitis (AC) and AC+adenosine group (AC+AD). AC was induced by rectal administration of 4% acetic acid (AA). 5mg/kg/day adenosine was performed i.p for 4 weeks to AC+AD group. Rectum and colon were excised for microscopic and histopathological histopathologic evaluations, and immunohistochemical analysis of nuclear factor kappa B (NF-kB). Blood samples were collected for biochemical detection of TNF-α, Pentraxin-3 and malondialdehyde (MDA) levels. RESULTS: AC group had generalized hyperemia and hemorrhage with increased macroscopic and histopathological scores compared with control (P <0.0001) while adenosine treatment decreased these scores significantly (P <0.001), with reduced distribution of disrupted epithelium, leukocyte infiltrates, and focal hemorrhage. AC group showed significantly increased immunoexpression of NF-kB in rectum, plasma and tissue levels of TNF-α, plasma Pentraxin-3 and MDA levels (P <0.0001) while adenosine reduced these levels (P < 0.05). CONCLUSION: Adenosine appears to promote healing of colon and rectum exposed to AA-induced AC, suggesting a boosting effect of adenosine on the intestinal immune system to cure ulcerative colitis.


Asunto(s)
Adenosina/uso terapéutico , Antiinflamatorios/uso terapéutico , Colitis Ulcerosa/tratamiento farmacológico , Ácido Acético , Enfermedad Aguda , Animales , Proteína C-Reactiva/análisis , Colitis Ulcerosa/inducido químicamente , Colitis Ulcerosa/patología , Colon/patología , Ensayo de Inmunoadsorción Enzimática , Inmunohistoquímica , Masculino , Malondialdehído/sangre , FN-kappa B/análisis , Ratas Sprague-Dawley , Recto/patología , Valores de Referencia , Reproducibilidad de los Resultados , Componente Amiloide P Sérico/análisis , Sustancias Reactivas al Ácido Tiobarbitúrico , Factores de Tiempo , Resultado del Tratamiento , Factor de Necrosis Tumoral alfa/análisis
7.
West Afr J Med ; 37(1): 26-31, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32030708

RESUMEN

BACKGROUND AND OBJECTIVES: Giant cell lesions (GCLs) are rare lesions which prominently feature multinucleated giant cells in their histology. They include central giant cell granuloma (CGCG), giant cell tumour of bone (GCT), peripheral giant cell granuloma (PGCG), Cherubism (CHB), e.t.c. This study reviewed the clinico-demographic parameters of GCLs of the jaws and assessed the giant cells. METHODS: This was a retrospective study examining the histopathology records of which part of the body of two tertiary institutions. All entries of cases diagnosed as GCLs were retrieved and data were extracted. Also, the giant cells in tissue sections were assessed. Data were analysed using SPSS Inc. version 20 while Chi square test was used to test for association. This was considered significant quand p < 0.05. RESULTS: Over the study period, 2,862 biopsy reports were reviewed. GCLs constituted 48(1.7%) and M: F ratio was 1:1.6 while majority occurred in the 2nd and 3rd decades. The mandible was the most common site recording 27(56.3%) cases and CGCG was the most frequently diagnosed GCL constituting 22(45.8%). Assessment of the giant cells revealed CGCG had predominantly large giant cells, a dense dispersal of giant cells and irregularly shaped giant cells, while CHB mainly had large giant cells with dense dispersal, but round shaped giant cells. CONCLUSION: GCLs are rare lesions commonly seen in females in the 2nd and 3rd decades of life with preference for the mandible. CGCG was the most commonly encountered lesion, while the giant cells in CGCG and CHB were similar in size as well as dispersal.


Asunto(s)
Granuloma de Células Gigantes/patología , Enfermedades Maxilomandibulares/patología , Adulto , Biopsia , Femenino , Células Gigantes , Humanos , Inmunohistoquímica , Estudios Retrospectivos
8.
Medicina (B Aires) ; 80(1): 81-83, 2020.
Artículo en Español | MEDLINE | ID: mdl-32044744

RESUMEN

Wiskott-Aldrich syndrome is a rare X chromosome-linked primary immunodeficiency syndrome associated with an increased incidence of infections, autoimmune disorders and neoplasms. We present the case of a 41-year-old man with a diagnosis of Wiskott-Aldrich syndrome with ileitis as a form of presentation of a lymphoproliferative syndrome. The ileitis, in the context of the patient, represents a clinical challenge given the large number of differential diagnoses (inflammatory bowel disease, infections, neoplasms and lymphoproliferative diseases), so it usually requires anatomopathological diagnosis and particular considerations regarding the subsequent specific treatment.


Asunto(s)
Neoplasias del Íleon/patología , Ileítis/patología , Linfoma/patología , Síndrome de Wiskott-Aldrich/patología , Adulto , Biopsia , Diagnóstico Diferencial , Humanos , Neoplasias del Íleon/diagnóstico , Ileítis/diagnóstico , Inmunohistoquímica , Linfoma/diagnóstico , Masculino , Síndrome de Wiskott-Aldrich/diagnóstico
9.
J Korean Med Sci ; 35(5): e31, 2020 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-32030920

RESUMEN

BACKGROUND: Mechanism and predictive biomarkers for tyrosine kinase inhibitor (TKI) resistance of advanced clear cell renal cell carcinoma (ccRCC) have not been fully evaluated. METHODS: We performed gene expression profiling on samples from an acquired TKI resistance cohort that consisted of 10 cases of TKI-treated ccRCC patients with matched tumor tissues harvested at pre-treatment and TKI-resistant post-treatment periods. In addition, a public microarray dataset from patient-derived xenograft model for TKI-treated ccRCC (GSE76068) was retrieved. Commonly altered pathways between the datasets were investigated by Ingenuity Pathway Analysis using commonly regulated differently expressed genes (DEGs). The significance of candidate DEG on intrinsic TKI resistance was assessed through immunohistochemistry in a separate cohort of 101 TKI-treated ccRCC cases. RESULTS: TNFRSF1A gene expression and tumor necrosis factor (TNF)-α pathway were upregulated in ccRCCs with acquired TKI resistance in both microarray datasets. Also, high expression (> 10% of labeled tumor cells) of TNF receptor 1 (TNFR1), the protein product of TNFRSF1A gene, was correlated with sarcomatoid dedifferentiation and was an independent predictive factor of clinically unfavorable response and shorter survivals in separated TKI-treated ccRCC cohort. CONCLUSION: TNF-α signaling may play a role in TKI resistance, and TNFR1 expression may serve as a predictive biomarker for clinically unfavorable TKI responses in ccRCC.


Asunto(s)
Biomarcadores de Tumor , Carcinoma de Células Renales , Resistencia a Antineoplásicos , Neoplasias Renales , Inhibidores de Proteínas Quinasas , Receptores Tipo I de Factores de Necrosis Tumoral , Transducción de Señal , Factor de Necrosis Tumoral alfa , Adulto , Biomarcadores de Tumor/metabolismo , Carcinoma de Células Renales/diagnóstico , Carcinoma de Células Renales/metabolismo , Carcinoma de Células Renales/terapia , Femenino , Humanos , Inmunohistoquímica , Neoplasias Renales/diagnóstico , Neoplasias Renales/metabolismo , Neoplasias Renales/terapia , Masculino , Persona de Mediana Edad , Pronóstico , Inhibidores de Proteínas Quinasas/farmacología , Inhibidores de Proteínas Quinasas/uso terapéutico , Receptores Tipo I de Factores de Necrosis Tumoral/metabolismo , Análisis de Supervivencia , Resultado del Tratamiento , Factor de Necrosis Tumoral alfa/metabolismo
10.
Cancer Treat Rev ; 85: 101992, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32092618

RESUMEN

Liquid biopsies (LB) are emerging in the oncology field, with promising data as new diagnostic, prognostic and treatment-monitoring tools. Squamous cell carcinoma of the head and neck (SCCHN) is a heterogenous disease and many challenges remain to improve patient outcomes. Liquid biopsy could be of interest at different stages of SCCHN disease, including better screening to diagnose more patients at an early stage, early detection of relapse after curative treatment, and the implementation of precision medicine. As LB is very attractive by the ease of sampling, this field is moving fast. Therefore, it is important to be aware of the potential applications but also the limitations of these new tools in regards to technical aspects and interpretation of the data. In this review, we will first give an overview of potential clinical applications and technical challenges of circulating tumor DNA (ctDNA) and then focus on current available data of ctDNA in SCCHN. Although the literature on ctDNA analysis for SCCHN is scarce compared to other tumors, preliminary results seem to hold promise for the future, including the detection of minimal residual disease or the detection of potentially targetable events through liquid biopsy. Prospective liquid-biopsy driven clinical trials are needed to validate its clinical relevance.


Asunto(s)
Biomarcadores de Tumor/genética , ADN Tumoral Circulante/sangre , Neoplasias de Cabeza y Cuello/genética , Neoplasias de Cabeza y Cuello/patología , Carcinoma de Células Escamosas de Cabeza y Cuello/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Biopsia con Aguja , Femenino , Humanos , Inmunohistoquímica , Masculino , Monitoreo Fisiológico/métodos , Invasividad Neoplásica/patología , Estadificación de Neoplasias , Pronóstico , Sensibilidad y Especificidad
11.
Sud Med Ekspert ; 63(1): 53-55, 2020.
Artículo en Ruso | MEDLINE | ID: mdl-32040089

RESUMEN

Aim of this study is to establish a possibility of finding morphologic signs of diffuse axonal injury early after the injury. Use of immunohistochemical examination of the brain to detect protein ß-APP made it possible not only to diagnose this condition correctly, but also to reasonably and categorically answer the question of a causal relationship between causing damage and the onset of death.


Asunto(s)
Lesiones Encefálicas , Lesión Axonal Difusa , Precursor de Proteína beta-Amiloide , Axones , Encéfalo/patología , Lesiones Encefálicas/diagnóstico , Lesiones Encefálicas/patología , Causas de Muerte , Lesión Axonal Difusa/diagnóstico , Lesión Axonal Difusa/patología , Humanos , Inmunohistoquímica
12.
Anticancer Res ; 40(2): 645-652, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32014905

RESUMEN

BACKGROUND/AIM: In estrogen receptor (ER)-positive, human epidermal growth factor receptor 2 (HER2)-negative breast cancer, standard chemotherapies as well as adjuvant endocrine therapy might not be enough for prevention of early relapse. MATERIALS AND METHODS: We focused on ER-positive, HER2 immunohistochemistry (IHC) 0 or 1+ breast cancer, and retrospectively examined HER2 gene amplification and TP53 mutation in breast cancer tissues in patients with or without early recurrence. Post-relapse survival in patients with early recurrence was also analyzed by mutation status of HER2 and TP53. RESULTS: Surprisingly, amplification of the HER2 gene was found in 15% of patients with early recurrence. None of the patients without relapse had HER2-amplified tumors. Post-relapse survival in patients with HER2 gene amplification and/or TP53 mutation in primary tumors was shorter than that in patients without these mutations, especially among postmenopausal women. CONCLUSION: HER2 gene amplification exists in ER-positive, HER2 IHC 0 or 1+ breast cancer in patients who developed early distant metastasis.


Asunto(s)
Neoplasias de la Mama/genética , Recurrencia Local de Neoplasia/genética , Receptor ErbB-2/genética , Receptores Estrogénicos/metabolismo , Adulto , Anciano , Neoplasias de la Mama/enzimología , Neoplasias de la Mama/patología , Estudios de Casos y Controles , Femenino , Amplificación de Genes , Humanos , Inmunohistoquímica , Persona de Mediana Edad , Mutación , Recurrencia Local de Neoplasia/enzimología , Recurrencia Local de Neoplasia/patología , Estadificación de Neoplasias , Receptor ErbB-2/metabolismo , Receptores Estrogénicos/biosíntesis , Estudios Retrospectivos , Proteína p53 Supresora de Tumor/genética
13.
Anticancer Res ; 40(2): 709-718, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32014912

RESUMEN

BACKGROUND/AIM: Prostate cancer (PCa) is the second most commonly diagnosed cancer in men. In contrast to localized disease, metastatic PCa leads to increased mortality. Kisspeptin (KISS1) functions as a metastasis suppressor in various cancers. The aim of this study was to detect the expression of KISS1 and its receptor GPR54 (KISS1R) in prostate cancer. MATERIALS AND METHODS: The expression of KISS1 and KISS1R was examined in prostate cancer tissue specimens after radical prostatectomy. RESULTS: A higher expression of KISS1 and KISS1R was shown in patients with localized tumors (Stage ≤IIb) compared to patients with advanced (Stage ≥III) tumor. High Gleason score PCa and higher prognostic groups patients showed a lower expression rate of both KISS1 and KISS1R. CONCLUSION: A down-regulation of KISS1-KISS1R system was detected in advanced prostate cancer. KISS1as tumor suppressor might be useful in the future for the diagnosis, risk assessment of prostate cancer progression, as well as a therapeutic target for aggressive tumors.


Asunto(s)
Kisspeptinas/biosíntesis , Neoplasias de la Próstata/metabolismo , Receptores de Kisspeptina-1/biosíntesis , Anciano , Humanos , Inmunohistoquímica , Kisspeptinas/metabolismo , Masculino , Persona de Mediana Edad , Clasificación del Tumor , Estadificación de Neoplasias , Neoplasias de la Próstata/genética , Neoplasias de la Próstata/patología , Receptores de Kisspeptina-1/genética , Receptores de Kisspeptina-1/metabolismo
14.
Braz Oral Res ; 34: e007, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32049108

RESUMEN

The aim of this study was to assess the influence of cyclosporine administration on the repair of critical-sized calvaria defects (CSDs) in rat calvaria filled with diverse biomaterials. Sixty animals were divided into two groups: the control (CTR) group (saline solution) and the cyclosporine (CCP) group (cyclosporine, 10 mg/kg/day). These medications were administered daily by gavage, beginning 15 days before the surgical procedure and lasting until the day the animals were euthanized. A CSD (5 mm Ø) was made in the calvaria of each animal, which was allocated to one of 3 subgroups, according to the biomaterial used to fill the defect: coagulum (COA), deproteinized bovine bone (DBB), or biphasic calcium phosphate ceramics of hydroxyapatite and ß-phosphate tricalcium (HA/TCP). Euthanasia of the animals was performed 15 and 60 days after the surgical procedure (n = 5 animals/period/subgroup). Bone repair (formation) assessment was performed through microtomography and histometry, while the analyses of the expression of the BMP2, Osteocalcin, and TGFß1 proteins were performed using immunohistochemistry. The CSDs not filled with biomaterials demonstrated lower bone formation in the CCP group. At 15 days, less bone formation was observed in the CSDs filled with DBB, a smaller volume of mineralized tissue was observed in the CSDs filled with HA/TCP, and the expression levels of BMP2 and osteocalcin were lower in the CCP group compared to the CTR group. The use of cyclosporine impaired bone repair in CSD, and this effect can be partially explained by the suppression of BMP2 and osteocalcin expression.


Asunto(s)
Regeneración Ósea/efectos de los fármacos , Sustitutos de Huesos/farmacología , Inhibidores de la Calcineurina/farmacología , Ciclosporina/farmacología , Osteogénesis/efectos de los fármacos , Animales , Proteína Morfogenética Ósea 2/análisis , Inmunohistoquímica , Masculino , Osteocalcina/análisis , Distribución Aleatoria , Ratas , Reproducibilidad de los Resultados , Cráneo/efectos de los fármacos , Cráneo/patología , Factores de Tiempo , Factor de Crecimiento Transformador beta1/análisis , Microtomografía por Rayos X
15.
Zhonghua Bing Li Xue Za Zhi ; 49(2): 116-121, 2020 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-32074722

RESUMEN

Objective: To investigate the expression of H3.3 G34W mutant-specific antibody in giant cell tumors of bone (GCTB), and its value in the diagnosis of GCTB. Methods: Immunohistochemical (IHC) EnVision method was used to detect the expression of H3.3 G34W mutant-specific antibody and p63 in 83 GCTBs, 18 aneurysmal bone cysts, 23 chondroblastomas and 28 osteosarcomas diagnosed at Nanjing Jinling Hospital from June 2001 to April 2019. Results: Among the 83 cases of GCTB, 69 cases (69/83, 83.1%) expressed H3.3 G34W. H3.3 G34W expression was found exclusively in the mononuclear cell population with strong and diffuse nuclear staining. H3.3 G34W was expressed in 55 of 57 (96.5%) cases of GCTB in long bones, but only 14 of 26 (53.8%) cases of non-long bone GCTB. All recurrent (9/9)/metastatic GCTB (2/2), post-denosumab GCTB (3/3), primary malignant GCTB (3/3) and secondary malignant GCTB (5/5) also expressed H3.3 G34W. H3.3 G34W was negative in all aneurysmal bone cysts and chondroblastomas. H3.3 G34W was positive in 3 of 28(10.7%) cases of osteosarcomas, and giant cell-rich osteosarcoma(GCRO) was the only histological subtype of osteosarcoma that expressed H3.3 G34W. p63 was expressed in 71.1%(59/83) of GCTB, while the positive rates of p63 in aneurysmal bone cysts,chondroblastomas and osteosarcomas were 3/18, 43.5% (10/23) and 21.4% (6/28) respectively. The sensitivity and specificity of H3.3 G34W mutant-specific antibody in the diagnosis of GCTB were 83.1% and 95.7%. Conclusions: H3.3 G34W mutant-specific antibody is a highly sensitive and specific marker for GCTB and helpful for the diagnosis of GCTB and its variants. The limitation of this antibody is that as a mall number of GCTB harbor G34 mutation other than G34W, and thus that cannot be detected. The incidental expression of H3.3 G34W mutant protein in osteosarcoma could be a potential diagnostic dilemma, and the results of H3.3 G34W IHC staining needs careful interpretation.


Asunto(s)
Neoplasias Óseas , Tumor Óseo de Células Gigantes , Neoplasias Óseas/diagnóstico , Condroblastoma , Tumor Óseo de Células Gigantes/diagnóstico , Histonas , Humanos , Inmunohistoquímica
16.
Zhonghua Bing Li Xue Za Zhi ; 49(2): 129-133, 2020 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-32074724

RESUMEN

Objective: To investigate the clinicopathological characteristics, histogenesis, immunophenotypes, molecular genetic characteristics, diagnosis and differential diagnosis of calcifying fibrous tumors (CFT). Methods: A total of 32 cases of CFT (22 cases from Henan Provincial People's Hospital and 10 cases from PLA Army Medical Center) diagnosed between June 2009 and February 2019 were reviewed. The clinical and pathologic data were analyzed. Results: There were 12 male and 20 female patients, aged from 15 to 63 years (mean 40.8 years). Eleven cases occurred in stomach, four cases in retroperitoneum, four cases in ovary, two cases in scrotum, two cases in mediastinum, two cases in head and neck, one case each in thoracic cavity, lung, adrenal gland, kidney, sigmoid colon, epididymis and mesosalpinx. All the tumors were solid masses with clear boundaries. The maximal dimension of the tumors ranged from 0.6 to 10.0 cm. Microscopically, there was hypocellular stromal sclerosis and wavy storiform coarse collagen with superimposed scattered or patchy lymphocytes and plasma cells; calcification or gravel formation were also detected. Immunohistochemistry showed that spindle cells were positive for vimentin and some were positive for CD34; and they were negative for calponin, SMA, desmin, S-100 protein, SOX10, STAT6, ß-catenin, ALK, CD117, DOG1, CKpan, and EMA. No ALK rearrangement was detected by FISH in all cases. No C-KIT and PDGFRA mutation was detected in all the tested 11 cases of stomach, four cases of retroperitoneal and one case of sigmoid colon CFT. MDM2 was not amplified by FISH in all four tested cases of retroperitoneal CFT. Conclusions: CFT is a rare benign tumor of fibroblastic cell origin. The diagnosis mainly depends on histomorphologic analysis and immunophenotyping. CFT should be differentiated from other benign and malignant spindle cell mesenchymal tumors.


Asunto(s)
Neoplasias de Tejido Fibroso , Adolescente , Adulto , Biomarcadores de Tumor , Diagnóstico Diferencial , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Proteínas Proto-Oncogénicas c-kit , Neoplasias Retroperitoneales , Vimentina , Adulto Joven
17.
Zhonghua Bing Li Xue Za Zhi ; 49(2): 134-138, 2020 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-32074725

RESUMEN

Objective: To investigate the clinicopathological manifestations, molecular genetic, diagnostic histology and differential diagnosis of alveolar soft part sarcoma (ASPS) in children. Methods: A total of 13 cases of ASPS diagnosed at Beijing Children's Hospital from August 2009 to November 2018 were collected. HE staining, histochemical staining for PAS and D-PAS, immunohistochemical (IHC) staining for TFE3, INI1 and CD68 and florescence in situ hybridization (FISH) for TFE3 gene translocation were performed. Results: There were four males and nine females, age ranged from 1 year and 2 months to 13 years and 8 months (mean 7.8 years); and four patients were under 5 years old. Histologically, the tumors showed a distinctive and characteristic nested or organoid growth pattern (11 cases) or solid, diffuse growth (2 cases). The tumor cells possessed abundant eosinophilic, or glycogen-rich and clear to vacuolated cytoplasm. The chromatin was relatively dispersed, with prominent and pleomorphic nucleoli; mitotic figures were rare. Vascular invasion was frequently seen. IHC staining showed specific nuclear TFE3 staining. The tumor cells were also positive for INI1,CD68 and vimentin; but were negative for MyoD1, Myogenin, CK and S-100 protein. Seven cases showed PAS and D-PAS staining, with fuchsia acicular or rod-shaped crystals in tumor cytoplasm. Nine cases showed TFE3 break-apart signals by FISH. Conclusions: ASPS is a rare soft tissue sarcoma in children. Compared with ASPA in adults, it has both similarities and unique clinicopathologic characteristics. The diagnosis needs to be confirmed by combining clinical, pathologic, IHC and genetic testing.


Asunto(s)
Sarcoma de Parte Blanda Alveolar , Adolescente , Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice , Niño , Preescolar , Femenino , Humanos , Inmunohistoquímica , Hibridación Fluorescente in Situ , Lactante , Masculino , Neoplasias de los Tejidos Blandos
18.
Zhonghua Bing Li Xue Za Zhi ; 49(2): 139-144, 2020 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-32074726

RESUMEN

Objective: To investigate the histopathologic, immunohistochemical, molecular genetic characteristics of dedifferentiated liposarcomas with meningothelial-like whorls(DDLMW). Methods: Six cases of DDLMW diagnosed at Jiangsu Province Hospital(the First Affiliated Hospital of Nanjing Medical University) from March 2012 to August 2018 were enrolled. The cases were analyzed by routine HE staining, immunohistochemistry(MDM2, CDK4 and p16) and fluorescent in-situ hybridization(FISH) on MDM2 gene. Related literatures were also reviewed. Results: Three of the 6 patients were male.The patient ages ranged from 40 to 77 years (mean, 58 years). Four tumors occurred in the retroperitoneum and two in the mediastinum. Histologically, the tumors showed, in addition to foci of well-differentied liposarcoma, characteristic, scattered meningothelial-like concentrical whorls. The whorls were composed of tightly, concentrically arranged, spindle to ovoid cells with mild to mederate cytological atypia. Metaplastic bone was present within or in their immediate vicinity in four case. The tumors cell also showed strong and diffuse immunoreactivity to MDM2, CDK4 and p16, but no immunoreactivity to S-100 protein, SMA, SOX10, EMA, CD21, CD23 or CD35. The Ki-67 labeling indexes were low, while FISH showed high levels of MDM2 amplification in all cases. Conclusions: DDLMW is a rare morphologic variant of dedifferentiated liposarcoma. The whorls in DDLMW do not represent perineurial or follicular dendritic differentiation. Recognition and familiarity with its existence, as well as combined application of immunohistochemical staining and MDM FISH, are important to avoid confusion with other lesions.


Asunto(s)
Liposarcoma , Adulto , Anciano , Biomarcadores de Tumor , Humanos , Inmunohistoquímica , Hibridación Fluorescente in Situ , Masculino , Persona de Mediana Edad , Proteínas Proto-Oncogénicas c-mdm2 , Proteínas S100
19.
Zhonghua Bing Li Xue Za Zhi ; 49(2): 145-148, 2020 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-32074727

RESUMEN

Objective: To detect the clinicopathological features, immunophenotype, diagnosis, and differential diagnosis of composite pheochromocytoma(CP). Methods: Five cases of CP were collected at Zhejiang Provincial People's Hospital from January 2011 to January 2019. The clinical, radiological, histologic, immunohistochemical and outcome data were analyzed; the diagnosis and differential diagnosis were discussed. Results: The patients' age range was 52-68 years (mean 59 years, median 54 years), There were 4 males and 1 female, and the male to female ratio was 4∶1. Tumor size was 3-4 cm (mean 3.6 cm, median 3.5 cm). The most common clinical manifestation was adrenal mass. Histologically, the classical feature was two distinct morphologic components, one with tumor cells arranged in irregular nests, and with fine granular and basophilic oramphophilic cytoplasm; the other was composed of scattered ganglion cells in the background of Schwann cells organized in interwoven bundles. The components of pheochromocytoma expressed PHOX2B(5/5), synaptophysin (5/5), CgA (5/5), the sustentacular cells expressed S-100 protein; the components of ganglioneuroma expressed S-100 protein (5/5), NF (5/5), the ganglion cells were weakly positive for PHOX2B, synaptophysin and CgA. All the cases were surgically resected and all patients were free of recurrence at follow-up. Conclusions: CP is rare adrenal tumor, and it has typical histologic features but no specific clinical manifestations. Attention should be paid to its characteristic histomorphology with the use of PHOX2B, CgA, synaptophysin and S-100 protein immunohistochemistry that is helpful for its diagnosis.


Asunto(s)
Neoplasias de las Glándulas Suprarrenales , Feocromocitoma , Anciano , Citoplasma , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Proteínas S100
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