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1.
Sci Rep ; 11(1): 2941, 2021 02 03.
Artículo en Inglés | MEDLINE | ID: mdl-33536486

RESUMEN

In recent months, Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world. COVID-19 patients show mild, moderate or severe symptoms with the latter ones requiring access to specialized intensive care. SARS-CoV-2 infections, pathogenesis and progression have not been clearly elucidated yet, thus forcing the development of many complementary approaches to identify candidate cellular pathways involved in disease progression. Host lipids play a critical role in the virus life, being the double-membrane vesicles a key factor in coronavirus replication. Moreover, lipid biogenesis pathways affect receptor-mediated virus entry at the endosomal cell surface and modulate virus propagation. In this study, targeted lipidomic analysis coupled with proinflammatory cytokines and alarmins measurement were carried out in serum of COVID-19 patients characterized by different severity degree. Serum IL-26, a cytokine involved in IL-17 pathway, TSLP and adiponectin were measured and correlated to lipid COVID-19 patient profiles. These results could be important for the classification of the COVID-19 disease and the identification of therapeutic targets.


Asunto(s)
/patología , Metabolismo de los Lípidos/fisiología , Alarminas/sangre , Citocinas/sangre , Análisis Discriminante , Femenino , Humanos , Análisis de los Mínimos Cuadrados , Lípidos/sangre , Masculino , Persona de Mediana Edad , Índice de Severidad de la Enfermedad
2.
Medicine (Baltimore) ; 100(5): e23955, 2021 Feb 05.
Artículo en Inglés | MEDLINE | ID: mdl-33592849

RESUMEN

BACKGROUND: Overweight before pregnancy is independent risk factor for diabetes mellitus. This randomized controlled trial was to investigate the long-term effects of regular exercise during pregnancy on overweight and obese gravidas. METHODS AND ANALYSIS: This single-center, prospective, randomized controlled test will be conducted in Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology. Overweight and obese pregnant women will be included in our study and randomized into 2 groups: regular exercise and control groups. The informed consent will be acquired in each patient. Body weight, body fat, fasting and 2 h glucose level in 75 g oral glucose tolerance test (OGTT), insulin resistance index, and lipid profiles were compared. We also evaluated their physical activities with International Physical Activity Questionnaire (IPAQ), their dietary habits with modified Adult Dietary Behavior Assessment Scale, and depression condition with Postpartum Depression Screen Scale (PDSS). The significance level was defaulted as P < .05. RESULTS: Results will be published in relevant peer-reviewed journals. CONCLUSION: Our study aims to systematically assess the effects of regular exercise for overweight and obese gravidas, which will be provided clinical guidance for overweight and obese gravidas.


Asunto(s)
Terapia por Ejercicio/métodos , Ejercicio Físico/fisiología , Obesidad/terapia , Sobrepeso/terapia , Complicaciones del Embarazo/terapia , Adulto , Peso Corporal/fisiología , Dieta/estadística & datos numéricos , Ayuno/fisiología , Conducta Alimentaria/fisiología , Femenino , Prueba de Tolerancia a la Glucosa , Humanos , Resistencia a la Insulina , Lípidos/sangre , Obesidad/fisiopatología , Sobrepeso/fisiopatología , Embarazo , Complicaciones del Embarazo/fisiopatología , Atención Prenatal/métodos , Estudios Prospectivos , Ensayos Clínicos Controlados Aleatorios como Asunto , Resultado del Tratamiento , Adulto Joven
3.
PLoS Pathog ; 17(2): e1009243, 2021 02.
Artículo en Inglés | MEDLINE | ID: mdl-33524041

RESUMEN

The current pandemic emergence of novel coronavirus disease (COVID-19) poses a relevant threat to global health. SARS-CoV-2 infection is characterized by a wide range of clinical manifestations, ranging from absence of symptoms to severe forms that need intensive care treatment. Here, plasma-EDTA samples of 30 patients compared with age- and sex-matched controls were analyzed via untargeted nuclear magnetic resonance (NMR)-based metabolomics and lipidomics. With the same approach, the effect of tocilizumab administration was evaluated in a subset of patients. Despite the heterogeneity of the clinical symptoms, COVID-19 patients are characterized by common plasma metabolomic and lipidomic signatures (91.7% and 87.5% accuracy, respectively, when compared to controls). Tocilizumab treatment resulted in at least partial reversion of the metabolic alterations due to SARS-CoV-2 infection. In conclusion, NMR-based metabolomic and lipidomic profiling provides novel insights into the pathophysiological mechanism of human response to SARS-CoV-2 infection and to monitor treatment outcomes.


Asunto(s)
Anticuerpos Monoclonales Humanizados/administración & dosificación , Lipidómica , Lípidos/sangre , /metabolismo , /sangre , Femenino , Humanos , Masculino , Resonancia Magnética Nuclear Biomolecular
4.
Medicine (Baltimore) ; 100(4): e24433, 2021 Jan 29.
Artículo en Inglés | MEDLINE | ID: mdl-33530244

RESUMEN

BACKGROUND: The improvement effect of exercise on diabetes mellitus has been widely recognized. Taijiquan, as a popular exercise mode for middle-aged and elderly people, is not clear about its effect on glucose and lipid metabolism in elderly diabetic patients. In this paper, the influence of Taijiquan on glucose and lipid metabolism in middle-aged and elderly diabetic patients was studied by using a meta-analysis method, to provide evidence for the clinical promotion of Taijiquan to improve glucose and lipid metabolism in diabetic patients. METHODS: Computer system search and manual search were conducted respectively in web of science, PubMed, Cochrane Library, EMBASE, Google Scholar, China National Knowledge Infrastructure, Wanfang Database, VIP from the inception to August 1, 2020. Randomized controlled trials of the application of Taijiquan in middle-aged and elderly diabetic patients were collected. RESULTS: The current study is a systematic review and meta-analysis program with no results. Data analysis will be completed after the program. CONCLUSION: This review aims to study the effect of Taijiquan on the glucose and lipid metabolism of middle-aged and elderly diabetic patients, objectively evaluate the effect of Taijiquan on the glucose and lipid metabolism of middle-aged and elderly diabetic patients, and provide scientific basis for clinical exercise intervention in middle-aged and elderly diabetic patients. PROTOCOL REGISTRATION NUMBER: INPLASY2020120107.


Asunto(s)
Glucemia/metabolismo , Diabetes Mellitus/terapia , Lípidos/sangre , Tai Ji/métodos , Anciano , Anciano de 80 o más Años , Diabetes Mellitus/sangre , Femenino , Humanos , Metabolismo de los Lípidos , Masculino , Metaanálisis como Asunto , Persona de Mediana Edad , Proyectos de Investigación , Revisiones Sistemáticas como Asunto , Resultado del Tratamiento
5.
Medicine (Baltimore) ; 100(2): e23743, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33466125

RESUMEN

INTRODUCTION: Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by high blood sugar caused by impaired insulin action. With an increasing incidence year by year, it has become a worldwide epidemic. Because of its serious, long-term condition, T2DM has a bad impact on the life and well-being of individuals, families and society. Renshen and Huanglian or compound prescription contain Renshen and Huanglian for treatment of T2DM has already been confirmed. However, due to the lack of evidence, there is no specific method or suggestion, so it is necessary to carry out systematic evaluation on Renshen and Huanglian and provide effective evidence for further research. METHODS AND ANALYSIS: We will search English databases (PubMed, Embase, Web of Science, Nature, Science on line, the Cochrane Library) and Chinese databases (CNKI, Wan Fang, VIP, Chinese biomedical database), from the establishment of database to October 2020, for randomized controlled trials (RCTs) of ginseng and coptis and the compound containing ginseng and coptis in the treatment of T2DM. Primary outcomes: fasting blood-glucose (FBG), 2 Hours Postprandial Blood Glucose (2hPBG), Glycosylated hemoglobin A1c (HbA1c). Additional outcomes: Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL), triglycerides (TG), total serum cholesterol (TC). Two researchers independently extracted the data and evaluated the quality of the included research, and meta-analysis was conducted on the included data using the software of RevMan5.3 and Stata V.12.0. RESULTS: The results of this study will systematically evaluate the effectiveness and safety of Renshen and Huanglian intervention for people with T2DM. CONCLUSION: The systematic review of this study will summarize the current published evidence of Renshen and Huanglian or compound prescription contain Renshen and Huanglian for the treatment of T2DM, which can further guide the promotion and application of it. ETHICS AND DISSEMINATION: This study is a systematic review; the outcomes are based on the published evidence, so examination and agreement by the ethics committee are not required in this study. We intend to publish the study results in a journal or conference presentations. OPEN SCIENCE FRAMEWORK OSF REGISTRATION NUMBER: October 18, 2020. osf.io/8gz7c (https://osf.io/8gz7c).


Asunto(s)
Diabetes Mellitus Tipo 2/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Hipoglucemiantes/uso terapéutico , Panax , Glucemia/efectos de los fármacos , Quimioterapia Combinada , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/efectos adversos , Hemoglobina A Glucada/efectos de los fármacos , Humanos , Hipoglucemiantes/administración & dosificación , Hipoglucemiantes/efectos adversos , Estilo de Vida , Lípidos/sangre , Ensayos Clínicos Controlados Aleatorios como Asunto , Proyectos de Investigación
6.
Ecotoxicol Environ Saf ; 208: 111682, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396014

RESUMEN

Iodine is important in both thyroid function and lipid metabolism. Some studies have explored the effect of thyroid hormones (THs) and urinary iodine concentration (UIC) on serum lipid levels. However, the association between iodine intake and dyslipidemia has not been well established. This study aimed to investigate the relationship between water iodine concentration (WIC) and dyslipidemia, including hypercholesterolemia, hypertriglyceridemia, low high-density lipoprotein cholesterol (HDL-C) and high low-density lipoprotein cholesterol (LDL-C). A cross-sectional survey was conducted involving 409, 390 and 436 adults (≥18 years) from the iodine-deficient (median water iodine, MWI < 10 µg/L), iodine-adequate (MWI between 40 and 100 µg/L) and iodine-excess (MWI > 100 µg/L) areas, respectively. WIC, total cholesterol (TC), triglyceride (TRIG), HDL-C and LDL-C were measured. The prevalence of dyslipidemia were calculated based on the level of WIC using the chi-square method. To further explore whether prevalence was associated with WIC, simple linear regressions and multiple logistic regression models were used. Compared to those with WIC of 40-100 µg/L, a WIC of >100 µg/L was found to be protective associated with against the occurrence of hypertriglyceridemia [adjusted odds ratio (AOR) = 0.649, 95% confidence interval (CI): 0.455-0.924] and low HDL-C (AOR = 0.429, 95% CI: 0.264-0.697). The prevalence of hypertriglyceridemia, low HDL-C and high LDL-C as a function of WIC was found to be an inverted U-shaped association with a zenith at a WIC of 40-100 µg/L. Collectively, our research showed that serum lipid levels are related to WIC. The benefit effect association between WIC and dyslipidemia appears in cases of iodine excess (>100 µg/L).


Asunto(s)
Agua Potable/química , Dislipidemias/epidemiología , Yodo/análisis , Lípidos/sangre , Adulto , China , HDL-Colesterol/sangre , Estudios Transversales , Agua Potable/normas , Dislipidemias/sangre , Femenino , Humanos , Metabolismo de los Lípidos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Oportunidad Relativa , Prevalencia , Encuestas y Cuestionarios , Triglicéridos/sangre
7.
Eur J Endocrinol ; 184(1): 179-187, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33112275

RESUMEN

Objective: Due to the rarity of Cushing's syndrome (CS) in children and adolescents, data are scarce about BMI during active disease and following remission. Therefore, our aim was to analyze BMI after long-term remission and determine predictive factors for promptly identifying patients at risk of being overweight or obese after remission for CS. Design: Retrospective cohort study. Patients: 73 patients: 58 (79.4%) had Cushing disease, 40 males (58%), median age of 12 years (IQR: 9-15). The mean follow-up time was 22.4 ± 18.2 months (range: 4-98). Methods: Main outcome measures: BMI, lipid profile, blood pressure, HOMA-IR. Results: At diagnosis, only eight (11%) patients had a normal weight. Although the BMI z-score at the last follow-up improved (2.0 ± 0.7 to 1.0 ± 1.2, P < 0.001), 44% remained overweight or obese after 2 years of remission according to the Kaplan-Meier curves. The BMI z-scores at the last follow-up correlated only with HOMA-IR levels (r: 0.49, P = 0.027). We found two independent factors related to reaching a normal weight: BMI z-score at diagnosis (HR: 0.156, 95% CI: 0.038-0.644; P = 0.01) and BMI z-score change at 6 ± 2 months (HR: 2.980, 95% CI:1.473-6.028; P = 0.002), which had high accuracy when a cut-off of 0.5 was used for ROC analysis (AUC = 0.828 (0.67-0.97); P < 0.001). Conclusions: Children and adolescents with CS have a high risk of being overweight or obese after successful treatment for their disease. At risk patients can be identified quickly based on their baseline BMI and initial weight loss after surgery. Efforts should focus on adopting healthy diet and lifestyle in the immediate postoperative period.


Asunto(s)
Síndrome de Cushing/complicaciones , Sobrepeso/etiología , Obesidad Pediátrica/etiología , Adolescente , Adulto , Índice de Masa Corporal , Niño , Estudios de Cohortes , Femenino , Humanos , Estimación de Kaplan-Meier , Lípidos/sangre , Masculino , Sobrepeso/epidemiología , Obesidad Pediátrica/epidemiología , Valor Predictivo de las Pruebas , Adulto Joven
8.
Ecotoxicol Environ Saf ; 209: 111805, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33360787

RESUMEN

BACKGROUND: Residents of a large area of North-Eastern Italy were exposed for decades to high concentrations of perfluoroalkyl and polyfluoroalkyl substances (PFAS) via drinking water. Serum PFAS levels have been consistently associated with elevated serum lipids, but few studies have been conducted among pregnant women, and none has stratified analyses by trimester of gestation. Elevated serum lipid levels during pregnancy can have both immediate and long-lasting effects on pregnant women and the developing fetus. We evaluated the association between perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and perfluoro-hexanesulfonate (PFHxS) levels in relation to lipid profiles in highly-exposed pregnant women. METHODS: A cross-sectional analysis was conducted in 319 pregnant women (age 14-48 years) enrolled in the Regional health surveillance program. Non-fasting blood samples were obtained in any trimester of pregnancy and analyzed for PFOA, PFOS and PFHxS, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C). Low-density lipoprotein cholesterol (LDL-C) was calculated. The associations between ln-transformed PFAS (and categorized into quartiles) and lipids were assessed using generalized additive models. Analyses were adjusted for potential confounders and stratified according to pregnancy trimester. RESULTS: The geometric means of PFOA, PFOS and PFHxS were 14.78 ng/mL, 2.67 ng/mL and 1.89 ng/mL, respectively. The plasma levels of TC, HDL-C and LDL-C increased steadily throughout the trimesters. In the 1st trimester, PFOS was positively associated with TC and PFHxS with HDL-C. In the 3rd trimester, instead, an inverse relationship was seen between PFOA and PFHxS and both TC and LDL-C. CONCLUSIONS: Results suggest the associations between PFAS concentrations and lipid profiles in pregnant women might differ by trimesters of pregnancy. In the first trimester, patterns are similar to those of non-pregnant women, while they differ late in pregnancy. Different independent behavior of PFAS and lipid levels throughout the pregnancy might explain our observations. These findings support the ubiquitous exposure to PFAS and possible influence on lipid metabolisms during pregnancy and suggest a careful evaluation of the timing of PFAS measurement, when examining effects of PFAS during pregnancy on gestational outcomes related to serum lipids amounts.


Asunto(s)
Contaminantes Ambientales/sangre , Fluorocarburos/sangre , Exposición Materna/estadística & datos numéricos , Mujeres Embarazadas , Adolescente , Adulto , Ácidos Alcanesulfónicos , Caprilatos , Estudios Transversales , Agua Potable , Femenino , Humanos , Italia , Lípidos/sangre , Persona de Mediana Edad , Embarazo , Adulto Joven
9.
Adv Exp Med Biol ; 1307: 115-128, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-32253710

RESUMEN

Diabetes mellitus (DM) is a metabolic disorder characterised by hyperglycemia and abnormalities in carbohydrate, fat and protein metabolism. Several studies demonstrated that foods typical of the Mediterranean diet (MedDiet), including vegetables, fruits, oilseeds, extra virgin olive oil and fish, can promote health benefits for individuals at risk of or with type 2 diabetes (T2DM). In this review, we summarised randomised clinical trials, cohort studies, meta-analyses and systematic reviews that evaluated the effects of the MedDiet on metabolic control of T2DM. The data suggest that the MedDiet influences cardiovascular risk factors, including blood pressure, lipid profile, insulin resistance, inflammation and glucose metabolism, in T2DM patients. In conclusion, the MedDiet appears to protect patients from macro- and microangiopathy and should be considering in the management of diabetic patients.


Asunto(s)
Diabetes Mellitus Tipo 2/dietoterapia , Dieta Mediterránea , Presión Sanguínea , Enfermedades Cardiovasculares/prevención & control , Glucosa/metabolismo , Humanos , Inflamación , Resistencia a la Insulina , Lípidos/sangre , Ensayos Clínicos Controlados Aleatorios como Asunto , Factores de Riesgo
10.
Ecotoxicol Environ Saf ; 207: 111269, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32911180

RESUMEN

This study is the first to examine the possible mechanism by which long-term exposure to permethrin (PER) can promote arterial retention of proatherogenic lipid and lipoproteins and related vascular dysfunction in rats. Experimental animals were administered two doses of oral PER, PER-1 (2.5 mg/kg/bw) and PER-2 (5 mg/kg/bw), for 90 consecutive days. The results indicated that both PER-1 and PER-2 increased plasmatic and aortic total cholesterol, low-density lipoprotein cholesterol (LDL-C), apo B-100, and oxidized LDL together with arterial scavenger LDL receptors (CD36) but markedly reduced plasmatic and hepatic high-density lipoprotein cholesterol and native LDL receptors in aortic and hepatic tissue. The levels of malondialdehyde, protein carbonyl, and reactive oxygen species were significantly higher, and glutathione content as well as catalase, superoxide dismutase, and glutathione peroxidase activities were suppressed in the aorta of the PER-1 and PER-2 groups. The arterial oxidative damage possibly caused by PER was clearly demonstrated by hematoxylin and eosin histological analysis. Moreover, PER treatment aggravated the inflammatory responses through enhancement of the production of proinflammatory cytokines (tumor necrosis factor-α, interleukin-2, and interleukin-6) in both plasma and aorta. Furthermore, PER-1 and PER-2 potentiated the dysregulation of the aortic extracellular matrix (ECM) content by increasing mRNA activation of collagens I and III. The abundant histological collagen deposition observed in the media and adventitia of intoxicated rats using Masson's trichrome staining corroborates the observed change in ECM. These data showed that oxidative stress related to PER exposure increases the arterial accumulation of lipoprotein biomarkers, likely by actions on both LDL and CD36 receptors, together with the disruption of the aortic ECM.


Asunto(s)
Colágeno/genética , Insecticidas/toxicidad , Lipoproteínas LDL/sangre , Estrés Oxidativo/fisiología , Permetrina/toxicidad , Animales , Aorta/metabolismo , Aorta/patología , Apolipoproteína B-100/metabolismo , Antígenos CD36/metabolismo , Inflamación/metabolismo , Lípidos/sangre , Masculino , Malondialdehído/metabolismo , Oxidación-Reducción , Ratas , Especies Reactivas de Oxígeno/metabolismo , Receptores de LDL/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo
11.
Artículo en Inglés | MEDLINE | ID: mdl-33317131

RESUMEN

Adjusted dietary assessment questionnaire was used to determine dietary habits of medical students which were related to biochemical and anthropometric markers of studied cohort. Thirty-seven young and healthy volunteers aged 19-28 years old entered the protocol and were divided according to sex and according to residence. Subjects were given questionnaires for tracking food/beverage consumption. Venous blood samples were taken after overnight fast (n = 32). Nutrient status and energy consumption were determined and analyzed. Study population had normal weight and body mass index (BMI). Biochemical characteristics were within normal reference range, while some participants had lipid profile disbalance. Men had significantly higher BMI than women. Average BMI was significantly higher in participants with elevated cholesterol levels compared to participants with normal cholesterol levels. Majority of participants consumed less than five meals per day with no major differences between students according to residence and sex. Men had significantly higher protein intake and consumed at least four meals daily compared to woman who had three or less meals daily with no differences in intake according to residence. Students with normal lipid profile consumed more carbohydrates than students with increased cholesterol. Results suggest that students with bad dietary habits have potentially higher risk for future cardiovascular problems, even before the onset of adverse effects.


Asunto(s)
Enfermedades Cardiovasculares , Ingestión de Alimentos , Conducta Alimentaria , Lípidos , Adulto , Antropometría , Índice de Masa Corporal , Enfermedades Cardiovasculares/sangre , Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/epidemiología , Estudios de Cohortes , Carbohidratos de la Dieta , Proteínas en la Dieta , Femenino , Humanos , Lípidos/sangre , Masculino , Adulto Joven
12.
PLoS One ; 15(12): e0243919, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33326441

RESUMEN

Common variants in the hepatic lipase (LIPC) gene have been shown to be associated with plasma lipid levels; however, the distribution and functional features of rare and regulatory LIPC variants contributing to the extreme lipid phenotypes are not well known. This study was aimed to catalogue LIPC variants by resequencing the entire LIPC gene in 95 non-Hispanic Whites (NHWs) and 95 African blacks (ABs) with extreme HDL-C levels followed by in silico functional analyses. A total of 412 variants, including 43 novel variants were identified; 56 were unique to NHWs and 234 were unique to ABs. Seventy-eight variants in NHWs and 89 variants in ABs were present either in high HDL-C group or low HDL-C group. Two non-synonymous variants (p.S289F, p.T405M), found in NHWs with high HDL-C group were predicted to have damaging effect on LIPC protein by SIFT, MT2 and PP2. We also found several non-coding variants that possibly reside in the circRNA and lncRNA binding sites and may have regulatory potential, as identified in rSNPbase and RegulomeDB databases. Our results shed light on the regulatory nature of rare and non-coding LIPC variants as well as suggest their important contributions in affecting the extreme HDL-C phenotypes.


Asunto(s)
HDL-Colesterol/sangre , LDL-Colesterol/sangre , Lipasa/genética , Afroamericanos , Alelos , Sitios de Unión/genética , Colesterol/sangre , Colesterol/genética , Proteínas de Transferencia de Ésteres de Colesterol/sangre , Proteínas de Transferencia de Ésteres de Colesterol/genética , HDL-Colesterol/genética , LDL-Colesterol/genética , Grupo de Ascendencia Continental Europea , Femenino , Genotipo , Humanos , Intrones/genética , Lipasa/ultraestructura , Metabolismo de los Lípidos/genética , Lípidos/sangre , Lípidos/genética , Masculino , Polimorfismo de Nucleótido Simple , Unión Proteica/genética , Conformación Proteica , ARN Circular/sangre , ARN Circular/genética , ARN Largo no Codificante/genética , Triglicéridos/sangre , Triglicéridos/genética
13.
Nat Commun ; 11(1): 6417, 2020 12 18.
Artículo en Inglés | MEDLINE | ID: mdl-33339817

RESUMEN

Pharmaceutical drugs targeting dyslipidemia and cardiovascular disease (CVD) may increase the risk of fatty liver disease and other metabolic disorders. To identify potential novel CVD drug targets without these adverse effects, we perform genome-wide analyses of participants in the HUNT Study in Norway (n = 69,479) to search for protein-altering variants with beneficial impact on quantitative blood traits related to cardiovascular disease, but without detrimental impact on liver function. We identify 76 (11 previously unreported) presumed causal protein-altering variants associated with one or more CVD- or liver-related blood traits. Nine of the variants are predicted to result in loss-of-function of the protein. This includes ZNF529:p.K405X, which is associated with decreased low-density-lipoprotein (LDL) cholesterol (P = 1.3 × 10-8) without being associated with liver enzymes or non-fasting blood glucose. Silencing of ZNF529 in human hepatoma cells results in upregulation of LDL receptor and increased LDL uptake in the cells. This suggests that inhibition of ZNF529 or its gene product should be prioritized as a novel candidate drug target for treating dyslipidemia and associated CVD.


Asunto(s)
Enfermedades Cardiovasculares/genética , Genoma Humano , Mutación con Pérdida de Función/genética , Terapia Molecular Dirigida , Bancos de Muestras Biológicas , Enfermedades Cardiovasculares/sangre , Silenciador del Gen , Marcación de Gen , Estudio de Asociación del Genoma Completo , Humanos , Lípidos/sangre , Hígado/metabolismo , Fenómica , Receptores de LDL/genética , Reino Unido
14.
BMC Surg ; 20(1): 329, 2020 Dec 14.
Artículo en Inglés | MEDLINE | ID: mdl-33317506

RESUMEN

BACKGROUND: Few articles have studied individuals with prediabetes after sleeve gastrectomy. Bile acid and lipid levels remain inconsistent in postbariatric patients. The purpose of this study was to explore bile acid, glucose, lipid, and liver enzyme changes in patients with different diabetes statuses who underwent sleeve gastrectomy. The impact of bariatric surgery and its potential benefits for prediabetic patients was also discussed. METHODS: A total of 202 overweight and obese patients who underwent bariatric surgery in our hospital between January 2016 and October 2018 were retrospectively reviewed. Patients were divided into prediabetes (n = 32), nondiabetes (n = 144), and diabetes (n = 26) groups and analysed. Glucose and lipid data were collected from medical records at baseline and at each follow-up visit. RESULT: Significant improvements in body weight, glucose and lipid levels, and liver enzymes (P ≤ 0.05) in prediabetic patients were found throughout the first year postoperatively. Improvement in glycaemic control was first seen one month postoperatively, followed by persistent improvement in the next 12 months. Total bile acid (TBA) decreased, which was associated with ALT improvement in prediabetic patients 1-year post-surgery. There were no significant differences in HbA1c, glucose, or triglycerides (TGs) between prediabetic and T2DM patients or between prediabetic and nondiabetic patients at 12 months post-surgery. CONCLUSION: LSG is highly effective at interfering with glucose and lipid levels as well as total bile acid levels in prediabetic patients in the first year postoperatively. Thus, LSG is indeed an alternative for overweight and obese prediabetic patients.


Asunto(s)
Ácidos y Sales Biliares , Glucemia , Diabetes Mellitus Tipo 2/cirugía , Gastrectomía/efectos adversos , Obesidad Mórbida/cirugía , Estado Prediabético/cirugía , Adulto , Diabetes Mellitus Tipo 2/sangre , Femenino , Humanos , Laparoscopía , Metabolismo de los Lípidos , Lípidos/sangre , Masculino , Persona de Mediana Edad , Estado Prediabético/sangre , Estudios Retrospectivos , Resultado del Tratamiento
15.
PLoS One ; 15(12): e0243538, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33362205

RESUMEN

INTRODUCTION: Some studies have reported the association between maternal serum lipid profile abnormalities and pre-eclampsia. However, many studies have reported controversial results. Hence, this systematic review and meta-analysis was planned to generate summarized evidence on the association between maternal serum lipid profiles and pre-eclampsia in African women. METHODS: Four electronic databases such as; PubMed, Hinari, Google Scholar, and African Journals Online were searched for studies published in English. Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument and Newcastle-Ottawa Scale were used for data extraction and quality assessment of the included studies. The meta- regression analysis was performed by Stata 14 software. The standardized mean difference (SMD) values of lipid profiles were computed to assess their association with pre-eclampsia at 95% CI. RESULTS: In this review a total of 15 observational studies were included. The mean values of triglyceride (TG), total cholesterol (TC), low density lipoprotein- cholesterol (LDL-c) and very low density lipoprotein- cholesterol (VLDL-c) were significantly higher in pre-eclamptic women as compared with normotensive pregnant women (TG = 229.61±88.27 and 147.00 ± 40.47, TC = 221.46 ± 45.90 and 189.67 ± 39.18, LDL = 133.92 ± 38.77 and 112.41 ± 36.08, VLDL = 41.44 ± 19.68 and 26.64 ± 7.87), respectively. The serum high density lipoprotein cholesterol (HDL-c) level was lower, but it is not statistically significant (HDL-c = 51.02 ± 16.01 and 61.80 ± 25.63) in pre-eclamptic women as compared with controls. The pooled standardized mean difference (SMD) of TG, TC, LDL-C and VLDL-C were significantly increased in pre-eclamptic women as compared with normotensive pregnant women with the SMD of (TG = 1.65 (1.10, 2.21), TC = 0.84 (0.40, 1.29), LDL-C = 0.95 (0.46, 1.45) and VLDL-C = 1.27 (0.72, 1.81)) at 95% CI, respectively, but the pooled SMD of HDL-cholesterol was decreased in pre-eclamptic women as compared with normotensive pregnant women (SMD = -0.91 (95% CI: -1.43, -0.39). CONCLUSIONS: In this review, the maternal serum levels of TG, TC, LDL-c and VLDL-c were significantly associated with the risk of preeclampsia. However, HDL- cholesterol was not significantly associated but it was lower in pre-eclamptic women. Further, large scale prospective studies should verify these outcomes and it is recommended that lipid profiles should be included as a routine diagnostic test for pre-eclamptic women.


Asunto(s)
Lípidos/análisis , Lípidos/sangre , Preeclampsia/epidemiología , Adulto , África/epidemiología , Presión Sanguínea , HDL-Colesterol/sangre , LDL-Colesterol/sangre , VLDL-Colesterol/sangre , Pruebas Diagnósticas de Rutina , Femenino , Humanos , Metabolismo de los Lípidos/fisiología , Persona de Mediana Edad , Preeclampsia/sangre , Embarazo , Mujeres Embarazadas , Estudios Prospectivos , Triglicéridos/sangre
16.
Medicine (Baltimore) ; 99(50): e23730, 2020 Dec 11.
Artículo en Inglés | MEDLINE | ID: mdl-33327364

RESUMEN

BACKGROUND: Atherosclerosis is the pathological basis of many cardiovascular and cerebrovascular diseases, and its pathogenesis is complex. Recent studies revealed a significant role of gut microbiota in the onset and development of atherosclerosis. Traditional Chinese medicine has rich clinical experience and unique advantages in the treatment of atherosclerosis. A large number of studies have proved that traditional Chinese medicine has the functions of reducing blood lipid, regulating gut microbiota, and resisting inflammation. The aim of this systematic review is to observe the randomized controlled trial of traditional Chinese medicine in treating gut microbiota, so as to evaluate the effectiveness and safety of traditional Chinese medicine in treating atherosclerosis patients. METHODS: The English database (PubMed, Web of Science, Embase, the Cochrane Library) and Chinese database (China National Knowledge Infrastructure, the Chongqing VIP Chinese Science, and Technology Periodic Database, Wanfang Database, and China Biomedical Literature Database) will be searched up to October 2020. We will also manually search the Chinese clinical trial register, conference papers, and unpublished studies or references. Randomized control trials of traditional Chinese medicine treatment of atherosclerosis were collected comprehensively, and 2 researchers will independently screen literature, data extraction, and evaluation the quality of literature methodology. The primary outcomes are lipid metabolism and gut microbiota and their metabolites. The secondary outcomes are the change of inflammatory markers. Meta-analysis was performed by RevMan 5.3.5 software. The Grades of Recommendation, Assessment, Development, and Evaluation will be used to evaluate the outcome quality of evidence. RESULTS: This study will comprehensively review the existing evidence of traditional Chinese medicine in treating atherosclerosis from the perspective of gut microbiota. CONCLUSION: This study will provide information on the effectiveness and safety of traditional Chinese medicine in treating atherosclerosis from the perspective of gut microbiota. UNIQUE INPLASY NUMBER: INPLASY2020110056.


Asunto(s)
Aterosclerosis/prevención & control , Aterosclerosis/fisiopatología , Medicamentos Herbarios Chinos/uso terapéutico , Microbioma Gastrointestinal/efectos de los fármacos , Biomarcadores , Quimioterapia Combinada , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/efectos adversos , Humanos , Mediadores de Inflamación/metabolismo , Lípidos/sangre , Ensayos Clínicos Controlados Aleatorios como Asunto , Proyectos de Investigación
17.
Nat Commun ; 11(1): 5698, 2020 11 10.
Artículo en Inglés | MEDLINE | ID: mdl-33173055

RESUMEN

Changes to lipid metabolism are tightly associated with the onset and pathology of Alzheimer's disease (AD). Lipids are complex molecules comprising many isomeric and isobaric species, necessitating detailed analysis to enable interpretation of biological significance. Our expanded targeted lipidomics platform (569 species across 32 classes) allows for detailed lipid separation and characterisation. In this study we examined peripheral samples of two cohorts (AIBL, n = 1112 and ADNI, n = 800). We are able to identify concordant peripheral signatures associated with prevalent AD arising from lipid pathways including; ether lipids, sphingolipids (notably GM3 gangliosides) and lipid classes previously associated with cardiometabolic disease (phosphatidylethanolamine and triglycerides). We subsequently identified similar lipid signatures in both cohorts with future disease. Lastly, we developed multivariate lipid models that improved classification and prediction. Our results provide a holistic view between the lipidome and AD using a comprehensive approach, providing targets for further mechanistic investigation.


Asunto(s)
Enfermedad de Alzheimer/metabolismo , Lipidómica , Lípidos/sangre , Biomarcadores/sangre , Estudios de Cohortes , Simulación por Computador , Humanos , Metabolismo de los Lípidos , Metabolómica
18.
Medicine (Baltimore) ; 99(46): e23130, 2020 Nov 13.
Artículo en Inglés | MEDLINE | ID: mdl-33181684

RESUMEN

BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the common gynecological endocrine system diseases. It is characterized by excessive androgen, rare or anovulation, and polycystic ovary morphology. Coenzyme Q10 (CoQ10) is a fat-soluble natural vitamin, which has a continuous oxidation-reduction cycle and is an effective antioxidant that can protect ovaries from oxidative damage. This study aims to systematically summarize and analyze the scientific literatures on glucose metabolism index, lipid profiles, inflammatory factor, and sex hormone level of PCOS patients treated with CoQ10 to provide a reference basis for clinical treatment. METHODS: We will retrieve the following electronic databases from the built-in until March 2021: Cochrane Library, PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), Clinical Trials. gov, Chinese Scientific Journal Database (VIP), and Wang-fang database. Two reviewers will independently scan the articles searched, de-duplication, filtering, quality assessment. Differences will be resolved by discussion between the 2 reviewers or by a third reviewers. All analyses were systematic to evaluate interventions based on the Cochrane handbook. Meta-analysis and/or subgroup analysis will be performed on the basis of the included studies. DISCUSSION: This review will be to investigate the efficacy of CoQ10 supplementation on glucose metabolism, lipid profiles, and biomarkers of inflammation in women with PCOS and provide a high-quality synthesis to assess whether CoQ10 is an effective and safe intervention for PCOS. The results of the analysis will be published in a scientific journal after peer-review. SYSTEMATIC REVIEW REGISTRATION: INPLASY 2020100013.


Asunto(s)
Glucemia/metabolismo , Inflamación/sangre , Lípidos , Síndrome del Ovario Poliquístico , Ubiquinona/análogos & derivados , Biomarcadores/sangre , Femenino , Humanos , Lípidos/sangre , Lípidos/clasificación , Metaanálisis como Asunto , Síndrome del Ovario Poliquístico/tratamiento farmacológico , Síndrome del Ovario Poliquístico/metabolismo , Proyectos de Investigación , Revisiones Sistemáticas como Asunto , Resultado del Tratamiento , Ubiquinona/farmacología , Vitaminas/farmacología
19.
Medicine (Baltimore) ; 99(46): e23211, 2020 Nov 13.
Artículo en Inglés | MEDLINE | ID: mdl-33181703

RESUMEN

Dietary intake influences gut microbiota activity. Nevertheless, there is a lack of evidence available that illustrates the acute effects of high glucose meal on metabolic endotoxemia. The present study assessed the acute impact of high glucose meal on endotoxemia and other clinical parameters in Saudi females with varying degrees of glycemia.The subjects were 64 consenting pre-menopausal women, grouped into 3: control [n = 14 lean, non-T2DM, BMI = 22.2 ±â€Š2.2 kg/m]; overweight [n = 16, non-T2DM, BMI = 28.5 ±â€Š1.5 kg/m] and T2DM [n = 34, BMI = 35.2 ±â€Š7.7 kg/m]. After an overnight fast, all subjects were given a standardized high-glucose (75 g) meal. Anthropometrics were taken and blood samples were withdrawn at baseline and postprandial (0, 2 and 4-hours), serum glucose, endotoxin and lipid profile were quantified.At baseline, total cholesterol, LDL-cholesterol, triglycerides and serum glucose levels were significantly higher (P values <.01) whereas significantly lower HDL-cholesterol levels (P < .01) were observed in T2DM subjects compared to other groups. Baseline endotoxin levels were highest in the overweight group (3.2 ±â€Š1.1 mmol/L) as compared to control (2.0 ±â€Š0.5 mmol/L) and T2DM (2.7 ±â€Š1.2 mmol/L) (P = .046). HDL-cholesterol, LDL-cholesterol and triglycerides, significantly decreased in the T2DM group after 2 hours (P values <.05), whereas unremarkable changes observed in other groups. Lastly, endotoxin levels significantly increased only in the overweight group (3.2 ±â€Š1.1 vs 4.2 ±â€Š1.4 mmol/L; P < .05), 4 hours postprandial.High glucose meal elevates endotoxemia only among overweight subjects and impairs dysbiosis.


Asunto(s)
Endotoxemia/complicaciones , Glucosa/análisis , Obesidad/complicaciones , Administración Oral , Adulto , Árabes/clasificación , Árabes/estadística & datos numéricos , Índice de Masa Corporal , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/fisiopatología , Endotoxemia/fisiopatología , Ayuno/sangre , Ayuno/metabolismo , Femenino , Humanos , Lípidos/análisis , Lípidos/sangre , Persona de Mediana Edad , Obesidad/fisiopatología , Prevalencia , Arabia Saudita
20.
Rev Col Bras Cir ; 47: e20202537, 2020.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-33053063

RESUMEN

OBJECTIVE: to analyze the effect of vertical gastrectomy on lipid profile and cardiometabolic risk in young women, preoperatively and 6 months after the operation. METHODS: retrospective study, encompassing medical record reviews of women's charts, preoperatively and in six months after the operation. Data collection was performed in the second half of 2015, using a review protocol with questions on the clinical-laboratory profile, anthropometric and laboratory classification of dyslipidemias. Descriptive and inferential analysis were used to treat the variables, using measures of variance, association and linear regression. RESULTS: we analyzed medical records of 114 women undergoing vertical gastrectomy, with a mean age of 33.82±10.92, and with complete high school education. There was a significant reduction of anthropometric data, as well as serum lipid values, six months after the surgical procedure. The coefficients of determination and the results of linear regression, showed that the reduction in serum triglyceride values and increase in high-density lipoprotein have a direct impact on the reduction of the cardiometabolic risk. Regarding the laboratory classification of dyslipidemias, it was observed that the majority presented a significant reduction at the six-month follow-up. Mixed hyperlipidemia showed no significant reduction. The categorized cardiometabolic risk showed a significant reduction in women at risk before vertical gastrectomy. CONCLUSION: at the six-month follow-up, vertical gastrectomy was effective in reducing the serum lipid profile and the cardiometabolic risk of young women when compared to the preoperative data. There was also a different improvement in the laboratory classification of dyslipidemias at the six-month follow-up after the surgical procedure.


Asunto(s)
Gastrectomía/efectos adversos , Lípidos/sangre , Obesidad Mórbida/cirugía , Enfermedades Cardiovasculares , Femenino , Humanos , Estudios Retrospectivos , Pérdida de Peso
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