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1.
Oral Health Prev Dent ; 18(1): 999-1010, 2020 11 20.
Artículo en Inglés | MEDLINE | ID: mdl-33215491

RESUMEN

PURPOSE: This study investigated the effect of long-term daily chemical disinfection on the topographic and Candida albicans biofilm formation on a denture base resin and a reline acrylic resin. MATERIAL AND METHODS: Circular samples (14 × 1.2 mm) were fabricated from a denture base (Vipi Wave) and reline acrylic resins (Tokuyama Rebase Fast II). Samples were kept in 50 ml of distilled water (48 h at 37°C). Subsequently, the samples were immersed in five different solutions: 0.5% sodium hypochlorite; 3.8% sodium perborate; 2% chlorhexidine gluconate; apple vinegar containing 4% maleic acid; and distilled water (control group). The specimen was immersed in the solutions for 8 h daily and transferred to distilled water at 37°C for more 16 h. The surface topographic and Candida albicans (ATCC 90028) biofilm formation were evaluated at baseline (before chemical disinfection) and after 1, 3 and 6 months of immersion. The surface topographic was evaluated by arithmetical roughness average (Ra) and scanning electron microscope (SEM), while the biofilm formation was evaluated by colony-forming units (CFU/ml) method and Alamar Blue assay (cell metabolism). The results were evaluated by three-way analysis of variance (ANOVAs) and post-hoc tests (α = 0.05). RESULTS: The results showed statistically significant effects from the type of acrylic resin (p = 0.029) and time (p <0.001) on the roughness of the specimen. In general, the reline resin had higher roughness than the denture base resin. In addition, the roughness of the samples after 1, 3 and 6 months of immersion in the cleaning solutions was higher than at baseline. In relation to the microbiological assays, there were no statistically significant differences (p >0.055) in the CFU/ml values of the biofilms among the different resins, periods of time and cleaning solutions. Considering the metabolism of the cells within the biofilms, the results showed that, at baseline, it was statistically significantly higher (p <0.05) than after 1, 3 and 6 months of storage. The SEM images showed that all disinfectant solutions provided surface changes of both acrylic resins (base and reline) after 1, 3 and 6 months of immersion. CONCLUSIONS: The roughness of both acrylic resins was affected by the disinfection in all cleaning agents, increasing over time, and this effect was more evident in the reline acrylic resin group. This surface change was also observed in the SEM images. While the number of cells within the biofilms was not affected by immersion in the cleaning agents, their metabolism was lower after 1, 3 and 6 months of immersion.


Asunto(s)
Candida albicans , Desinfección , Resinas Acrílicas , Biopelículas , Bases para Dentadura , Limpiadores de Dentadura/farmacología , Ensayo de Materiales , Propiedades de Superficie
2.
BMC Oral Health ; 20(1): 193, 2020 07 08.
Artículo en Inglés | MEDLINE | ID: mdl-32641017

RESUMEN

OBJECTIVE: To compare the antifungal action of two commercially available denture cleaning agents to that of standard clinical solutions, and determine their effects on the polymethyl methacrylate (PMMA) acrylic resin denture surface. METHODS: Candida albicans growth was analyzed by colony forming assay, and the methyl thiazolyl tetrazolium (MTT) assay was used to evaluate biofilm formation and cell adhesion. The morphology and roughness of PMMA acrylic resin surface was measured by scanning electron microscopy (SEM) images and stylus method. RESULTS: Clene®, Polident® and 3% NaHCO3 solutions showed significantly greater antifungal effects in terms of both inhibiting growth and biofilm formation. In addition, Clene® solution prevented adhesion of C. albicans on cell culture plates compared to filter-sterile tap water, whereas other reagents did not have an inhibitory effect. One-month immersion in the different cleaning reagents significantly inhibited fungal adhesion on the PMMA surface Clene®, Polident® and 3% NaHCO3 showed greater effect compared to PBS and filter-sterile tap water. Finally, none of the cleansing agents significantly affected the morphology and roughness of the PMMA surface. CONCLUSION: Clene®, Polident® and 3% NaHCO3 solutions can inhibit C. albicans growth and biofilm formation to some extent on cell culture plates, and significantly inhibit fungal adhesion on the PMMA surface without affecting surface morphology and roughness.


Asunto(s)
Biopelículas/efectos de los fármacos , Candida albicans/efectos de los fármacos , Materiales Dentales/química , Bases para Dentadura , Limpiadores de Dentadura/farmacología , Polimetil Metacrilato/química , Polimetil Metacrilato/farmacología , Biopelículas/crecimiento & desarrollo , Candida albicans/crecimiento & desarrollo , Humanos , Peróxidos , Propiedades de Superficie
3.
Int J Prosthodont ; 33(3): 315-320, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32320185

RESUMEN

PURPOSE: To evaluate the antimicrobial activity of effervescent tablets on the surfaces of cobalt-chromium (Co-Cr) and heat-polymerized resin. MATERIALS AND METHODS: From a metal matrix, 55 circular wax patterns (Ø 12 × 3 mm) were obtained and cast in Co-Cr alloy. Muffles for acrylic resin were prepared from circular wax patterns (Ø 20 × 5 mm). The metal specimens were positioned in the muffle, and the resin was pressed into its surroundings to simulate the composition of a removable partial denture (RPD). The mixed specimens were sterilized and contaminated with Streptococcus mutans, Staphylococcus aureus, Candida albicans, and Candida glabrata, composing a multispecies biofilm, and subsequently immersed according to the manufacturer's instructions in four cleansing solutions: Polident 3 Minute denture cleanser (P3M), Polident for Partials (PP), Corega Tabs (CT), and NitrAdine (Ni); as well as distilled water (positive control) and no contamination (negative control). After cleansing, viable microorganisms were quantified by counting the number of colony-forming units (CFU/mL). From the CFU values, log10(CFU + 1) values were calculated for statistical analysis. Kruskal-Wallis test with Dunn post hoc test were performed (α = .05). RESULTS: There was a significant reduction (P = .001) of S mutans after immersion in Ni (median [95% CI] 3.27 [2.92; 3.45]) compared to the CT (3.86 [3.75; 4.01]) and control (4.08 (3.73; 4.22]) groups, while the PP (3.63 [3.28; 4.11]) and P3M (3.83 [3.61; 4.04]) groups presented an intermediate action. The effervescent tablets did not present antimicrobial action against S aureus (P = .537), C albicans (P = .795), or C glabrata (P = .519). CONCLUSION: Ni exhibited moderate antimicrobial action. The effervescent tablets did not promote reduction of multispecies biofilm, and their daily use should be carefully considered.


Asunto(s)
Antiinfecciosos , Dentadura Parcial Removible , Biopelículas , Candida albicans , Limpiadores de Dentadura , Dentaduras , Streptococcus mutans , Propiedades de Superficie , Comprimidos
4.
Niger J Clin Pract ; 23(3): 304-309, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32134027

RESUMEN

Background: Denture hygiene is an important factor to remove dental plaque and discoloration, to provide optimal oral health, and to prevent denture stomatitis. Aim: The purpose of this study was to evaluate the effect of coffee staining and denture cleaner on the color stability of two differently polymerized (heat and microwave) denture acrylic resin in vitro. Materials and Methods: Two commonly used denture base acrylic resins and effervesecnt tablet form denture cleaner were used in this study. Disc-shaped specimens from each acrylic resin were polymerized according to the manufacturer's instructions (n = 7). Color values were recorded before and after immersion in distilled water (I), denture cleaner (II), coffee (III), and coffee + denture cleaner (IV) with a tristimulus colorimeter using CIEDE2000 color difference formula. Two-way analysis of variance (ANOVA) and Fisher's least significant difference tests were used for multiple comparisons at a 95% confidence level (P < 0.05). Surface topography of the acrylic resin denture base specimens before and after immersion were taken under 500 × magnification. Results: The two-way ANOVA revealed significant interactions between denture base resins and solutions (P < 0.001). Immersion in denture cleaner and coffee solution caused significant color changes in acrylic resin denture bases. Conclusion: Heat- and microwave-polymerized acrylic denture base resins can show different color stability. Immersion in coffee and denture cleaner solutions can cause noticeable color changes.


Asunto(s)
Resinas Acrílicas/química , Café , Materiales Dentales/química , Bases para Dentadura , Limpiadores de Dentadura/efectos adversos , Calor , Microondas , Decoloración de Dientes , Agua , Color , Colorimetría , Humanos , Inmersión , Ensayo de Materiales , Polimerizacion , Coloración y Etiquetado , Propiedades de Superficie
5.
J. oral res. (Impresa) ; 9(1): 72-80, feb. 28, 2020.
Artículo en Inglés | LILACS | ID: biblio-1151506

RESUMEN

Everyday use of denture cleansers to prevent microbial colonization on dentures can affect the properties of the denture base material. Limited literature is available on the effects of denture cleanser on reinforced resins. This study aimed to evaluate and compare the effect of denture cleanser on the flexural and impact strength of conventional and reinforced heat cure acrylic resins. Materials and Methods: Rectangular shaped specimens were prepared for flexural and impact strength as per ISO 1567. The denture base resins used included conventional, high impact, nylon fiber reinforced and glass fiber reinforced heat cure acrylic resins. They were further categorized into subgroups A and B depending on immersion in distilled water or denture cleanser (3.8% sodium perborate based). The flexural strength and impact strength of specimens were measured. The study design is a observational case-control study. SPSS version 25 statistical analysis software was used, and the STROBE statement checklist was followed. Results: The mean flexural strength was highest for glass fiber reinforced heat cure resin followed by conventional heat cure resin, high impact heat cure resin and nylon reinforced heat cure resin, respectively. The mean impact strength was highest for high impact heat cure resin followed by glass fiber reinforced heat cure resin, conventional heat cure resin, and nylon fiber-reinforced resin respectively.Conclusion: All the denture base resins tested exhibited a decrease in flexural and impact strength following use of a denture cleanser.


El uso diario de limpiadores para prótesis dentales para prevenir la colonización microbiana en las prótesis puede afectar las propiedades del material base de estas. Existe literatura limitada sobre los efectos de los limpiadores de prótesis sobre resinas reforzadas. Este estudio tuvo como objetivo evaluar y comparar el efecto de un limpiador de prótesis sobre la resistencia a la flexión y al impacto de las resinas acrílicas de curado con calor convencionales y reforzadas. Material y Métodos: Muestras de forma rectangular se prepararon para testear la resistencia a la flexión y al impacto según ISO 1567. Las resinas de base de prótesis utilizadas fueron resinas acrílicas convencionales, de alto impacto, y de curado térmico reforzadas con fibra de vidrio o nylon. Posteriormente se clasificaron en los subgrupos A y B según la inmersión en agua destilada o limpiador de prótesis (3,8% de perborato de sodio). Se midieron la resistencia a la flexión y la resistencia al impacto de las muestras. El diseño del estudio fue de un estudio observacional de casos y controles. Se utilizó el software de análisis estadístico SPSS versión 25, y se siguió la guía de la declaración STROBE. Resultado: La resistencia a la flexión fue más alta para la resina de curado por calor reforzada con fibra de vidrio seguida de la resina de curado por calor convencional, la resina de curado por calor de alto impacto y la resina de curado por calor reforzada con nylon, respectivamente. La resistencia al impacto fue más alta para la resina de curado por calor de alto impacto seguida de la resina de curado por calor reforzada con fibra de vidrio, la resina de curado por calor convencional y la resina reforzada con fibra de nylon, respectivamente. Conclusión: Todas las resinas de base de prótesis probadas exhibieron una disminución en la resistencia a la flexión y al impacto después del uso de un limpiador de prótesis.


Asunto(s)
Humanos , Bases para Dentadura , Limpiadores de Dentadura , Resistencia Flexional , Resinas Acrílicas , Prótesis Dental
6.
J Prosthodont ; 29(3): 237-242, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31943512

RESUMEN

PURPOSE: To evaluate and compare the alterations in retention of three Locator attachments after immersion in various denture cleansers at defined time intervals. MATERIALS AND METHODS: Two implants were embedded in an acrylic block. Pink, blue, and clear Locator attachments (n = 10 for each subgroup) were immersed into three different cleansing solutions (Corega, Protefix and NaOCl) and tap water (control) at different time intervals that simulate 1 (T1 ), 6 (T2 ), and 12 (T3 ) months of clinical use. Universal testing machine set at a crosshead speed of 50 mm/min was used for pull-out tests. After the immersion procedure, the peak force to dislodgement was recorded to demonstrate the changes in retention of Locator attachments. Data were analyzed using repeated-measures ANOVA followed by Tukey's Honestly Significant Difference (HSD) tests (α = .05). RESULTS: All denture cleansing solutions affected the retentive values of all Locator attachments at defined time intervals (F = 4.299, p = 0.001). NaOCl affected all groups significantly after 12-month immersion time (p = 0.001). The least retention loss at all subgroups was observed in Corega for clear Locator attachments (106.17 ± 5.21 N). The difference in retention values for pink attachments were not statistically significant for tap water, Protefix and Corega immersion at all time intervals (p > 0.05). Following NaOCl (blue; 33.31 ± 4.72 N, clear; 52.3 ± 8.5 N), tap water decreased the retention value of blue (41.14 ± 3.93 N) and clear (76.72 ± 8.42 N) Locator attachments at T3 . Corega caused the least retention loss to clear attachments at T1 (106.17 ± 3.55 N). CONCLUSION: The retention of Locator attachments decreases over time after exposure to various denture cleansing solutions. As NaOCl significantly decreased the retentive values of all attachments, patients must be informed about that disadvantage. Also, tap water remarkably decreased retentive ability of all attachments. Periodic addition of an effervescent tablet into tap water may be recommended to patients that wear implant retained overdentures with Locator attachments.


Asunto(s)
Retención de Dentadura , Prótesis de Recubrimiento , Prótesis Dental de Soporte Implantado , Análisis del Estrés Dental , Limpiadores de Dentadura , Humanos
7.
J Prosthodont Res ; 64(4): 373-379, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31787576

RESUMEN

PURPOSE: This study aims to investigate the effect of mechanical and chemical cleaning on the surface roughness of silicone soft relining materials. METHODS: We selected silicone soft relining materials with the highest (Soft) and lowest (Supersoft) Shore A hardness. In the abrasion test, specimens were cleaned 50,000 times using a kitchen sponge (Sponge), a soft (Soft brush) or hard (Hard brush) denture brush, or stored in water (No cleaning). In the immersion test, specimens were immersed in either water (Water), neutral peroxide denture cleanser (Neutral), alkaline peroxide denture cleanser (Alkaline), or hypochlorite denture cleanser (Hypochlorite) for 1440 h. Surface roughness of the arithmetic mean height of the surface (Sa) and maximum height (Sz) were measured before and after the tests. Data were analyzed using the Kruskal-Wallis and Mann-Whitney U tests. RESULTS: In the abrasion test, significant differences were observed for Sa and Sz with Soft relining materials, but not for No cleaning and Sponge. In the immersion test, significant differences were observed for Sa and Sz with Soft relining materials, but not between Water and Neutral or Water and Alkaline. Significant differences were observed with Supersoft, except between Water and Neutral or Water and Alkaline for Sa and between Water and Neutral for Sz. CONCLUSIONS: Mechanical cleaning using a sponge did not increase the surface roughness of the material with a high Shore A hardness. Furthermore, neutral peroxide denture cleanser did not increase the roughness of materials with high and low Shore A hardness.


Asunto(s)
Alineadores Dentales , Siliconas , Limpiadores de Dentadura , Ensayo de Materiales , Propiedades de Superficie
8.
Int J Prosthodont ; 33(1): 74-80, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31860916

RESUMEN

AIMS: To evaluate the effects of different cleansing solutions on the physical-mechanical properties (roughness, surface hardness, and fatigue resistance) of three polymeric materials used to manufacture retentive attachments for overdentures. MATERIALS AND METHODS: The roughness and surface hardness analyses each employed 150 specimens measuring . 9 mm × 2 mm in thickness (polyacetal, polytetrafluoroethylene [PTFE], and polyethylene terephthalate [PET]; n = 50 each). For fatigue resistance analysis, 180 retentive attachments measuring 4 mm × 3 mm in height (n = 60 each) were used. The properties were evaluated before and after immersion in different cleansing solutions: distilled water, alkaline peroxide, 0.5% sodium hypochlorite (NaOCl), and Listerine. After the data distribution was verified using Shapiro-Wilk test, parametric or nonparametric analysis was applied (α = .05). RESULTS: The use of NaOCl caused a significant alteration in the roughness of the materials (P = .011), with a reduction in roughness in polyacetal and an increase in PTFE. The type of solution also influenced the surface hardness (P = .036); with the exception of distilled water, the other solutions promoted increased hardness. During the 24-month period, immersion in water, peroxide, and NaOCl (0.5%) caused increased fatigue resistance (P < .05) of the PET attachments. The immersion protocol resulted in greater fatigue resistance (P < .05) in polyacetal, while PTFE was not affected (P > .05). CONCLUSION: Regarding the physical-mechanical properties evaluated, the polymers PET, polyacetal, and PTFE were susceptible to cleansing solutions.


Asunto(s)
Resinas Acrílicas , Limpiadores de Dentadura , Dentaduras , Ensayo de Materiales , Polímeros , Hipoclorito de Sodio , Propiedades de Superficie
9.
J Prosthet Dent ; 124(2): 176-182, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31787270

RESUMEN

STATEMENT OF PROBLEM: Overnight removal of complete dentures is recommended to reduce the incidence of denture stomatitis. The effect of overnight storage conditions is unclear. PURPOSE: The purpose of this systematic review was to assess the effect of overnight storage conditions on complete denture colonization by Candida albicans and to explore the effect of overnight storage conditions on the dimensional stability of complete dentures. MATERIAL AND METHODS: Two electronic databases were searched through to November 2018. The terms "denture*", "dental prosthes*", "acrylic resin*", "storage", "nighttime", "overnight", "wet", "dry", "water*", and "solution" were chosen. Articles meeting the inclusion criteria were selected. For both research questions, studies that did not store the dentures overnight or for a minimum of 8 hours were excluded. For the primary research question, studies that were not randomized controlled studies or comparative studies were excluded. RESULTS: The database search strategy resulted in a total of 159 potential studies. After screening titles and abstracts and applying the inclusion and exclusion criteria, 6 studies were retrieved for a full-text assessment. Hand searching was also performed. Four studies were included in the systematic review for the primary research question. Three studies were included for the secondary research question. A meta-analysis could not be performed because of variation in study design. CONCLUSIONS: Cleaning of dentures before overnight storage helps reduce C. albicans colonization. If the dentures are not cleaned, the use of an alkaline peroxide-based cleaning tablet should be considered. Alternately, overnight dry storage is an option for reducing C. albicans colonization, with clinically insignificant changes to the dimensions of the complete denture. Storing dentures in water alone may promote C. albicans colonization.


Asunto(s)
Biopelículas , Candida albicans , Dentadura Completa , Resinas Acrílicas , Limpiadores de Dentadura , Peróxidos
10.
J Prosthodont ; 29(2): 142-150, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30381847

RESUMEN

PURPOSE: To compare the effect of solutions of effervescent tablets (ET), cetylpyridinium chloride (CPC), and experimental solutions of Ricinus communis on the surface of cobalt-chromium (Co-Cr) alloys. MATERIALS AND METHODS: Fifty-five specimens of Co-Cr were prepared by the lost-wax casting method using circular patterns (∅12 × 3 mm). The specimens were randomly divided into 5 groups: deionized water (control); 2% R. communis; 10% R. communis; ET, and CPC. The surface roughness of specimens (n = 10) was evaluated before immersion (baseline), and at simulated times of ½, 1, 2, 3, 4, and 5 years, by laser confocal microscope (Sa, µm) and profilometer (Ra, µm). The surface topography and chemical composition (n = 1) was qualitatively analyzed with scanning electron microscopy (SEM), and energy dispersive X-ray spectrometry (EDS). Data were subjected to Kruskal-Wallis followed by Dunn tests, and Friedman followed by Wilcoxon tests (α = 0.05). RESULTS: For Sa, there was no difference for the solution factor. For the time factor a significant difference was found with 2% R. communis solution among baseline and ½, 2, 3, and 5 years (p < 0.001) and with 10% R. communis solution between 1 and 2 years (p = 0.007), with decreasing roughness over time. For Ra, cetylpyridinium chloride exhibited less roughness than 10% R. communis solution in ½ (p = 0.048) and 5 years (p = 0.013). In the SEM and EDS analysis the solutions did not present deleterious effects or changes in the chemical composition on the surfaces. CONCLUSIONS: Although a significant difference was found for the roughness, the results, below 0.2 µm, are clinically acceptable. Thus, all solutions can be used safely in removable partial denture cleaning for a period of 5 years.


Asunto(s)
Limpiadores de Dentadura , Dentadura Parcial Removible , Aleaciones de Cromo , Ensayo de Materiales , Propiedades de Superficie
11.
Am J Dent ; 32(4): 201-207, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31436941

RESUMEN

PURPOSE: To evaluate in vitro the effectiveness of eight different cleansers containing alkaline peroxide against yeast [Candida albicans (Ca) and Candida glabrata (Cg) ], Gram-negative bacteria [Escherichia coli (Ec) and Pseudomonas aeruginosa (Pa) ], and Gram-positive bacteria [ Bacillus subtilis (Bs), Enterococcus faecalis (Ef), Staphylococcus aureus (Sa), and Streptococcus mutans (Sm) ]. METHODS: Denture base acrylic resin specimens (Lucitone 550; 1264 denture base acrylic resin specimens) were obtained from a circular metal matrix (15 x 3 mm) and sterilized with microwave irradiation (650 W, 6 minutes). The specimens were contaminated with 106 CFU/mL suspension of yeasts and 108 CFU/mL suspensions of Gram-positive and -negative bacteria. The contaminated specimens were incubated (37°C/48 hours) and immersed (short cycle) in a stainless steel basket placed in a beaker. The beaker contained one of the following cleanser solutions, which were prepared and used according to the manufacturers' instructions (n=10): PBS (positive control, C), Fixodent (FI), Medical Interporous (MI), Kukident (KU), Efferdent Plus (EF), Equate (EQ), Kroger (KR), Kirari (KI), and Corega Tabs (CT). Reduction in the viability of microorganisms was evaluated through counting of Colony Forming Units (CFU/mL), metabolic activity (XTT), and cell viability by epifluorescence microscopy (EM) analyses. For counting CFU, the specimens (n=10 of each group) were washed and immersed in Letheen medium, from which aliquots were obtained and seeded into selective solid culture media. After incubation (37°C, 24 hours), the colonies were counted (CFU/mL). The metabolic activity of microorganisms was measured using XTT reduction assay (n=5). For EM analysis, the specimens (n= 2 of each group) were stained (Live/Dead BacLight), and the images were analyzed in terms of biofilm areas (total and living cells). Data were processed and analyzed by Wilcoxon, Mann Whitney and Kruskal-Wallis tests followed by Dunn test (α= 0.05). RESULTS: Significant differences were found between the experimental groups and C for the evaluated microorganisms. MI was the most effective for yeasts; this cleanser reduced the metabolic activity, viability, and total counts of both yeasts evaluated. All cleansers reduced the total counts of Gram-negative microorganisms, except CT for Pa. For Gram-negative bacteria, KR, CT, EF, and EQ significantly reduced the metabolic activity of Ec but did not remove all dead cells. FI and MI significantly reduced the metabolic activity of Pa, and the latter also removed the dead cells of Pa. FI and MI showed improved results against all Gram-positive bacteria. Both cleansers significantly decreased the metabolic activity of all bacterial species and reduced the viability of Sa, Ef, and Bs. MI also reduced the biofilm aggregates of Sm. CLINICAL SIGNIFICANCE: This study concluded that under the experimental conditions tested, denture cleansers can be used as coadjutants in denture cleaning. The evaluated cleansers exhibited disinfectant action, thereby preventing the proliferation of microorganisms on the denture surface and reducing the risk of opportunistic infections.


Asunto(s)
Biopelículas , Bases para Dentadura , Limpiadores de Dentadura , Peróxidos , Biopelículas/efectos de los fármacos , Recuento de Colonia Microbiana , Dentaduras , Peróxidos/farmacología
12.
BMC Oral Health ; 19(1): 71, 2019 05 02.
Artículo en Inglés | MEDLINE | ID: mdl-31046747

RESUMEN

BACKGROUND: This study evaluated the efficacy of four methods in removing Candida albicans from the acrylic base material used to fabricate removable orthodontic appliances. METHODS: Heat-processed bars of orthodontic acrylic were incubated in a suspension of C. albicans for 2 h at 37 °C. Samples were allocated into five groups (five bars per group) according to the cleaning method: (1) manual brushing using a toothbrush; (2) soaking in a commercial denture cleaning solution; (3) soaking in a commercial mouthwash solution; (4) using an ultrasonic cleaner; and (5) soaking in distilled water as a negative control. Yeast remaining attached to the bars after cleaning were removed by vortexing in growth medium and plated on Sabouraud dextrose agar. The reduction in yeast colony count after cleaning was calculated and expressed as the number of colony forming units per acrylic bar (CFU/bar). The experiment was carried out three times. RESULTS: All four cleaning methods resulted in a significant decrease in viable yeast cells associated with the acrylic bars compared to the control group. The mean percentage reduction in viable yeast cells affected by the cleaning methods was: brushing 89.9%; chlorhexidine 95.8%; ultrasonic cleaning 99.9%; and denture tablet 100%. CONCLUSIONS: All four methods evaluated in this study were effective, to some extent, in removing C. albicans from orthodontic acrylic samples. The most effective, and readily available, cleaning method was the use of commercial denture sterilizing tablets.


Asunto(s)
Resinas Acrílicas/química , Biopelículas/efectos de los fármacos , Candida albicans/efectos de los fármacos , Bases para Dentadura/microbiología , Limpiadores de Dentadura/farmacología , Dentadura Completa/microbiología , Higiene Bucal/métodos , Cepillado Dental/métodos , Recuento de Colonia Microbiana , Limpiadores de Dentadura/química , Desinfección/métodos , Humanos , Agua/química
13.
Int J Prosthodont ; 32(2): 196-197, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30856645

RESUMEN

PURPOSE: To experimentally examine the effectiveness of a small sample of commercial denture cleansers in removing Candida albicans cells from denture surfaces. MATERIALS AND METHODS: A total of 216 specimens from three brands of denture base resins (72 for each acrylic resin) were divided into three groups of 24 specimens that each received a different surface treatment (Ra1, Ra2, and Ra3). The specimens were contaminated by the Candida albicans strain ATCC 90028, immersed for 15 minutes in one of two experimental denture cleanser solutions or in tap water, and placed in Petri dishes with culture medium. RESULTS: Candida albicans colonies were measured after 24-hour incubation at 37ÆC. There was a statistically significant difference in the cleansing result depending on the denture cleanser used. CONCLUSION: The use of commercial denture cleansers may under certain conditions be effective in the removal of Candida albicans from denture bases.


Asunto(s)
Bases para Dentadura , Limpiadores de Dentadura , Resinas Acrílicas , Candida albicans , Polimetil Metacrilato , Propiedades de Superficie
14.
J Appl Biomater Funct Mater ; 17(1): 2280800019827797, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30803303

RESUMEN

INTRODUCTION:: We assessed the effect of different available denture cleansers on the roughness and hardness of polyetherketoneketone, thermoinjection-molded polyamide, and polymethylmethacrylate. MATERIALS AND METHODS:: A total of 150 disc-shaped specimens were fabricated (10 mm × 2 mm) from these three denture base resins, and divided into five subgroups ( n = 10) according to immersion procedures. One of these groups subjected to distilled water served as control, whereas other groups were subjected to daily cleansing with four denture cleansers (Corega, Protefix, Curaprox, and Perlodent) for 8 h a day for 140 days. The surface roughness and hardness values of specimens were recorded by measuring twice at baseline, and again after application of chemical solutions. Topography alterations after treatments were assessed with scanning electron microscopy. The data were subjected to statistical analysis and comparison among groups was done using Kruskal Wallis and Wilcoxon Signed Ranks tests. P-value <0.05 was considered significant. RESULTS:: The surface roughness of polyetherketoneketone, polymethylmethacrylate, and polyamide dentures was increased significantly by chemical solutions of denture cleansers. While the hardness value of polyetherketoneketone was not affected significantly after immersion in denture cleansers, those of polymethylmethacrylate and polyamide decreased significantly. Compared with Curaprox, the effervescent tablets significantly altered the surface hardness and roughness of polyamide. CONCLUSION:: Denture cleansers can considerably alter the surface roughness and hardness of denture base resins and should be used carefully depending on the material.


Asunto(s)
Limpiadores de Dentadura/química , Resinas Acrílicas/química , Dureza , Microscopía Electrónica de Rastreo , Nylons/química , Polimetil Metacrilato/química , Propiedades de Superficie
15.
J Prosthodont ; 28(9): 1011-1017, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30720223

RESUMEN

PURPOSE: The objective of this study was to improve the surface characteristics of poly (methyl methacrylate) (PMMA) by developing a novel, thin film coating process and to characterize the resulting coated surface. MATERIALS AND METHODS: An atomic layer deposition (ALD) technique was developed to deposit a titanium dioxide (TiO2 ) nano-thin film on PMMA. The surface wettability for both coated and uncoated PMMA was determined by measuring water contact angle. Wear resistance was assessed using a mechanical tooth-brushing device with a 50 g load for 6000 strokes after 5 months of water storage. A denture cleanser challenge test was performed by using sonication in 3.8% sodium perborate for 1 hour with aged specimens. X-ray photoelectron spectroscopy (XPS) was used before and after the brushing test and challenge test to analyze the PMMA surface chemical composition. The mechanical strength of coated and uncoated PMMA was measured using a three-point bending test. Surface microbial interactions were also evaluated by assessing Candida albicans biofilm attachment. RESULTS: Nano-TiO2 coating (30 nm thick) was successfully deposited on PMMA at 65°C. After coating, water contact angle decreased from 70° to less than 5°. After brushing test, the coating remained intact. XPS analysis revealed no loss of TiO2 from coated specimens following brushing and denture cleanser sonication for 1 hour. There was no statistically significant difference in mechanical strength (MPa) (mean ± SD) between PMMA (139.4 ± 11.3) and TiO2 -PMMA (160.7 ± 37.1) (p = 0.0995). C. albicans attachment decreased by 63% to 77% on the coated PMMA surface. CONCLUSIONS: ALD is a promising technique to modify surface properties of PMMA and resulted in a stable adherent thin film. By depositing a TiO2 coating, PMMA surface properties may lead to significantly reduced microorganism adhesion and easier pathogen removal from PMMA. For patients who wear dentures, reducing the oral microbial biofilm burden using a TiO2 -coated PMMA surface could positively impact their oral and systemic health.


Asunto(s)
Polimetil Metacrilato , Titanio , Limpiadores de Dentadura , Humanos , Ensayo de Materiales , Propiedades de Superficie
16.
Br Dent J ; 226(1): 2, 2019 01 11.
Artículo en Inglés | MEDLINE | ID: mdl-30631173
17.
Indian J Dent Res ; 30(6): 920-926, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31939372

RESUMEN

Background: Immersion-type denture cleansers are commonly used for denture hygiene maintenance. Hence, it is crucial to investigate the effect of denture cleansing solutions on bond strength between direct reline materials and denture base resin. Aims: This in vitro study aimed to determine the effect of denture cleansers on bond strength between direct hard reline materials and denture base resin. Materials and Methods: Cylindrical columns of hard-liners (Hard GC Reline, TDV Cold Liner Rebase, Tokuyama Rebase II Fast) were bonded to heat-polymerized denture base resin. A total of fifty specimens were fabricated for each reline material and divided into five groups (n = 10): Group I (control): No solution was used; Group II: Specimens were stored in distilled water for 60 days; Groups III, IV, and V: Specimens were stored in distilled water for 60 days with daily immersion in either sodium hypochlorite, calgon + sodium hypochlorite, or dentipur tablet for 5 min. The shear bond strength was examined at a cross-head speed of 1 mm/min. Failure mode was evaluated by stereomicroscope. Statistical Analysis: Data were analyzed by two-way ANOVA and Chi-square test (α=0.05). Results: The results showed no significant interaction between the direct hard-liners and denture cleansers (P = 0.119). Hard GC Reline had the highest bond strength, followed by Tokuyama Rebase II Fast, and then, TDV Cold Liner Rebase. No significant difference existed in bond strength between samples immersed in water and cleansers or between the cleansers themselves. Hard GC Reline had more mixed failure mode compared to TDV Cold Liner Rebase and Tokuyama Rebase II Fast. There was a significant correlation between mixed mode of failure and higher values of bond strength (P = 0.008). Conclusions: Within the limitations of the present study, denture cleansing solutions could not significantly influence the bond strength between hard direct liners and denture base resin.


Asunto(s)
Recubrimiento Dental Adhesivo , Alineadores Dentales , Resinas Acrílicas , Bases para Dentadura , Limpiadores de Dentadura , Rebasado de Dentaduras , Calor , Ensayo de Materiales
18.
Am J Dent ; 32(6): 306-310, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31920057

RESUMEN

PURPOSE: The efficacy of a propolis solution against denture biofilm was evaluated by means of an in vitro assessment and a cross-over randomized clinical trial. METHODS: Acrylic resin specimens were contaminated by Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Candida glabrata, Candida parapsilosis, immersed in a (A) propolis solution, (B) saline or (C) alkaline peroxide, applied onto Petri plates with culture medium and after incubation the number of colonies was counted. For the clinical trial, 30 complete denture wearers were randomly assigned to groups (A) propolis solution, and (B) saline, following one of the sequences (I)A/B or (II) B/A. After each intervention, biofilm was quantified by means of digital photos taken from the intaglio surface and a microbiological quantification of Candida spp. and mutans streptococci was conducted. RESULTS: Both propolis solution and alkaline peroxide reduced the microbial counts for S. mutans and C. albicans with significant and greater effect for group C (P< 0.05). However, no difference was found clinically between the interventions. The propolis solution showed an intermediate antimicrobial effect against S. mutans and C. albicans. Also, it did not exert an immediate effect on denture biofilm. CLINICAL SIGNIFICANCE: A commercially available propolis-based cleanser solution was evaluated in vitro and clinically for the treatment of denture stomatitis. Although an immediate effect on denture biofilm was not observed after a single application, It showed antimicrobial effect against S. mutans and C. albicans.


Asunto(s)
Limpiadores de Dentadura , Própolis , Biopelículas , Recuento de Colonia Microbiana , Dentadura Completa , Dentaduras , Streptococcus mutans
19.
J Prosthodont ; 28(2): e857-e862, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-29235702

RESUMEN

PURPOSE: To evaluate the effect of experimental (Ricinus communis) and commercial dentifrices used for denture cleaning on abrasiveness (gravimetric method; roughness), hardness, and color stability of a resilient relining material. MATERIALS AND METHODS: Sixty circular (15 × 3 mm) specimens were distributed into four groups: C (control; brushing with water); CO (brushing with Colgate - for natural teeth); CB (brushing with Corega Brite - for complete dentures); RC (brushing with experimental dentifrice). Brushing was performed in a toothbrushing machine with a soft brush and a dentifrice suspension for 50 minutes, calculated to correspond to 1 year of regular brushing. Variables were measured initially and after the trial period. For the gravimetric method, the difference in mass was considered. The surface roughness was measured by a rugosimeter, and the hardness test was performed by a Shore A durometer. Color changes (ΔE; CIE L*a*b* and NBS systems) were measured by a portable spectrophotometer. Results were analyzed by ANOVA/Tukey's test (α = 0.05). RESULTS: The largest mass variation (µg; p < 0.0001) occurred in C (-6.21 ± 3.18). Concerning roughness, CB (0.26 ± 0.04) showed the lowest value, followed by RC (0.29 ± 0.08) and CO (0.34 ± 0.24) (p < 0.0001). Group C produced the greatest surface roughness (0.72 ± 0.25). Hardness values decreased after brushing with water (p = 0.014). No significant differences were found among RC (50.31 ± 1.03), CO (49.11 ± 1.31), CB (49.17 ± 1.23), and C (48.02 ± 1.26). Color stability was similar in all groups (p = 0.135; C: 2.3 ± 0.77; CO: 2.6 ± 0.54; CB: 2.2 ± 0.44; RC: 2.9 ± 1.56). CONCLUSIONS: The use of experimental dentifrice could be indicated, as it showed similar results to the specific dentifrice, keeping the resilient material properties within acceptable values.


Asunto(s)
Dentífricos/química , Limpiadores de Dentadura/química , Alineadores Dentales , Cepillado Dental , Color , Dureza , Técnicas In Vitro , Ensayo de Materiales , Ricinus , Propiedades de Superficie
20.
J Prosthodont ; 28(2): 138-145, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30365198

RESUMEN

PURPOSE: To evaluate oral and dermal tolerance following use and user acceptability of an experimental denture-cleansing wipe. An exploratory objective was to develop a method to assess denture wipe effectiveness in removing debris from denture surfaces. MATERIALS AND METHODS: This was a single-center, randomized, controlled, parallel-group, examiner-blind study in participants with ≥1 full/partial denture. Participants were randomized to clean their dentures with the denture wipe (n = 76) or water (n = 76) up to 4 times per day for 14 days. Tolerability was assessed by treatment-emergent adverse events (TEAEs), oral soft tissue examination, and lead hand dermatological assessment. Acceptability was assessed by questionnaire. The feasibility of a methodology to assess the efficacy of the wipe at removing food particles was also evaluated through determination of the mass of chewed peanut particles that the wipe removed after a single use (n = 31). RESULTS: The proportion of participants experiencing oral TEAEs by day 14 was 0.039% with the denture wipe (lip injury [n = 1], mouth injury [n = 2]) and 0.013% with the water rinse (coated tongue [n = 1]). There were no dermal TEAEs and no TEAE-related study withdrawals. Skin irritation scores with the denture wipe remained unchanged from baseline. Comparing before vs. after cleaning with the denture wipe, a higher proportion of participants rated their dentures as feeling extremely/very fresh (28.9% pre-/85.5% post-cleaning), feeling extremely/very clean (34.2%/86.8%) and looking extremely/very clean (43.5%/85.5%). More denture-wipe group participants than water-rinse group participants were extremely/very satisfied with the amount of debris removed from their dentures (88.1% vs 72.4%). The methodology used to assess the weight of peanut particles captured from the wipes/dentures appeared to be a feasible investigation technique. CONCLUSIONS: The denture wipe was generally well-tolerated and had good user acceptability. The methodology for assessing the mass of peanut particles removed by denture wipes was successful.


Asunto(s)
Limpiadores de Dentadura , Higiene Bucal/instrumentación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Encuestas y Cuestionarios , Resultado del Tratamiento
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