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1.
Medicine (Baltimore) ; 99(18): e18755, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32358341

RESUMEN

Many inflammation indicators have been reported to be related with patient outcomes in various cancers. Previous studies have evaluated the combination of platelet (PLT) and lymphocyte to monocyte ratio (COP-LMR) as a systemic inflammatory marker for prognostication in lung cancer, yet its prognostic role among breast cancer patients remains unclear.In the present study, a total of 409 breast cancer patients with surgical resection were retrospectively investigated. The receiver operating characteristic (ROC) curve was used to choose the optimal cut-off value of PLT and lymphocyte to monocyte ratio (LMR). Patients were classified into 3 groups according to the score of COP-LMR, and its relationship with various clinicopathological factors and breast cancer prognosis were further evaluated.The ROC curve analysis showed that COP-LMR had a higher area under the ROC curve for the prediction of 5-year disease-free survival and overall survival than PLT or LMR alone. Multivariable analysis showed that an elevated COP-LMR was an independent predictor of poor disease-free survival (P = .032) and overall survival (P = .005). Subgroup analysis revealed that COP-LMR was still significantly associated with prognosis in both luminal A and luminal B subtypes.Preoperative COP-LMR is a potential prognostic factor in breast cancer patients who underwent surgery.


Asunto(s)
Neoplasias de la Mama/mortalidad , Recuento de Leucocitos/estadística & datos numéricos , Linfocitos , Monocitos , Recuento de Plaquetas/estadística & datos numéricos , Adulto , Anciano , Neoplasias de la Mama/sangre , Neoplasias de la Mama/cirugía , Supervivencia sin Enfermedad , Femenino , Humanos , Mastectomía , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Periodo Preoperatorio , Pronóstico , Curva ROC , Estudios Retrospectivos
2.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(3): 333-336, 2020 Mar 30.
Artículo en Chino | MEDLINE | ID: mdl-32376581

RESUMEN

OBJECTIVE: To identify the biomarkers as early warning signals for severe COVID-19. METHODS: We retrospectively analyzed the clinical data of 63 patients with COVID- 19 from Hubei Provincial Hospital of Integrated Chinese and Western Medicine, including 32 moderate cases and 31 severe cases. The demographic data, underlying diseases, clinical manifestations and laboratory test results were compared between the two groups. Logistic regression analysis was performed to identify the factors that predicted the severity of COVID-19. The receiver- operating characteristic curve (ROC) of neutrophil/lymphocyte ratio (NLR) was calculated, and the area under the curve (AUC) was determined to estimate the optimal threshold of NLR for predicting severe cases of COVID-19. RESULTS: The patients with moderate and server COVID-19 showed significant differences in the rate of diabetes, NLR, serum amyloid A (SSA), C-reactive protein (CRP) and serum albumin (ALB) levels (P < 0.05). The co- morbidity of diabetes, NLR, SSA and CRP were found to positively correlate and ALB to inversely correlate with the severity of COVID-19 (P < 0.05). Multivariate logistic regression analysis showed that NLR was an independent risk factor for severe COVID-19 (OR=1.264, 95% CI: 1.046-1.526, P=0.015) with an AUC of 0.831 (95% CI: 0.730-0.932), an optimal diagnostic threshold of 4.795, a sensitivity of 0.839, and a specificity of 0.750. CONCLUSIONS: An increased NLR can serve as an early warning signal of severe COVID-19.


Asunto(s)
Betacoronavirus , Infecciones por Coronavirus , Linfocitos , Neutrófilos , Pandemias , Neumonía Viral , Humanos , Recuento de Leucocitos , Pronóstico , Curva ROC , Estudios Retrospectivos
3.
Isr Med Assoc J ; 22(5): 294-298, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32378821

RESUMEN

BACKGROUND: Bariatric surgery has become the most common and effective therapeutic option for obesity. However, it is associated with morbidity and complications. Identification of predictors for surgical complications is an unmet need. OBJECTIVES: To determine a simple non-invasive parameter that predicts early postoperative complications following bariatric surgery. METHODS: In this retrospective study of all patients who underwent elective bariatric surgery at Nazareth Hospital EMMS during a 4-year period (2015-2018). We collected clinical and laboratory parameters and determined predictors of complications. RESULTS: A total of 345 patients underwent bariatric surgery during the study period. Of the patients, 51 experienced early post-bariatric surgery complications as compared to 294 patients who had no complications. Univariate analysis revealed that neutrophil-to-lymphocyte ratio (NLR) (odds ratio [OR] 1.912, P < 0.0001) and platelet to lymphocyte ratio (OR 1.015, P < 0.0001) were associated with post-bariatric surgery complications. In a multivariate logistic regression analysis, only NLR remained a significant predictor (OR 1.751, 95% confidence interval 1.264-2.425, P = 0.0008) with a receiver operating characteristic curve for NLR of 0.8404. CONCLUSIONS: We found that the NLR predicts post bariatric surgery early complications. Further prospective studies are needed to validate our findings.


Asunto(s)
Cirugía Bariátrica , Linfocitos , Neutrófilos , Obesidad/sangre , Obesidad/cirugía , Complicaciones Posoperatorias/sangre , Adulto , Femenino , Humanos , Recuento de Leucocitos , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Periodo Preoperatorio , Estudios Retrospectivos
6.
Anticancer Res ; 40(4): 2311-2317, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32234931

RESUMEN

BACKGROUND/AIM: Elevated neutrophil-lymphocyte ratio (NLR) has been reported to be a poor prognostic factor in patients with colorectal cancer (CRC). However, no studies have focused on the dynamic change of preoperative NLR (pre-NLR) in CRC patients. We investigated the prognostic value of the change in NLR (ΔNLR) in CRC patients before and after surgery. PATIENTS AND METHODS: We retrospectively analyzed the data from 307 patients with stage II or III CRC. We compared the clinicopathological factors, OS, and DFS among the various NLR factors. RESULTS: The 5-year OS rate of the high ΔNLR group was significantly lower than that of the low ΔNLR group (p<0.01). The 5-year DFS rates of the high ΔNLR groups were worse than those in the low ΔNLR groups. In the multivariate analysis, ΔNLR was an independent prognostic factor (p=0.011). CONCLUSION: Decreasing post-NLR was related to better OS and DFS even in high pre-NLR patients with CRC.


Asunto(s)
Neoplasias Colorrectales/cirugía , Recuento de Linfocitos , Linfocitos/patología , Neutrófilos/patología , Anciano , Anciano de 80 o más Años , Neoplasias Colorrectales/sangre , Neoplasias Colorrectales/patología , Femenino , Humanos , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Análisis Multivariante , Estadificación de Neoplasias , Periodo Preoperatorio , Pronóstico , Estudios Retrospectivos
7.
Anticancer Res ; 40(4): 2343-2349, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32234936

RESUMEN

BACKGROUND/AIM: The host's systemic inflammatory response is thought to affect the progression of cancer and the antitumor effects of chemotherapy. Meta-analyses have reported that the peripheral blood platelet-to-lymphocyte ratio (PLR) is a prognostic indicator of this effect. Therefore, we hypothesized that PLR may differ, depending on sentinel lymph node metastasis (SLNM) in patients diagnosed with cT1N0M0 breast cancer by preoperative imaging. This study investigated the ability of preoperative PLR to predict SLNM in patients diagnosed with cT1N0M0 breast cancer. PATIENTS AND METHODS: This study included 475 patients with cT1N0M0 breast cancer diagnosed by preoperative imaging. Peripheral blood was obtained at diagnosis, i.e., before surgery. PLR was calculated from preoperative blood tests, by dividing the absolute platelet count by the absolute lymphocyte count. RESULTS: The probability of SLNM was significantly higher (p=0.002) in cases where the tumor diameter was larger than 10 mm. The incidence of SLNM was significantly high in the high (preoperative) PLR group (p=0.031). Multivariate analysis revealed that high PLR [compared to low PLR, p=0.021, odds ratio (OR)=1.815, 95% confidence interval (CI)=1.093-3.090] and large tumor size (compared to small tumor size, p=0.001, OR=2.688, 95%CI=1.524-4.997) were independent factors influencing SLNM. CONCLUSION: PLR may act as a predictor of SLNM in cT1N0M0 breast cancer.


Asunto(s)
Plaquetas/patología , Neoplasias de la Mama/cirugía , Linfocitos/patología , Ganglio Linfático Centinela/patología , Adulto , Anciano , Neoplasias de la Mama/sangre , Neoplasias de la Mama/patología , Femenino , Humanos , Metástasis Linfática , Recuento de Linfocitos , Persona de Mediana Edad , Análisis Multivariante , Evaluación de Resultado en la Atención de Salud , Recuento de Plaquetas , Periodo Preoperatorio , Pronóstico
8.
Mutat Res ; 850-851: 503143, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32247554

RESUMEN

BACKGROUND: The cytokinesis-block micronucleus (CBMN) assay is an internationally recognized method for measuring DNA damage after exposure to genotoxic agents, as well as a biomarker for DNA repair and chromosomal instability. The high baseline level of micronuclei (MN) in the healthy population has limited the sensitivity and application of the CBMN assay for the follow-up of exposed populations. We reevaluated the sensitivity of the CBNM assay using semi-automated MN scoring following telomere and centromere (TC) staining after in vitro exposure to genotoxic agents (mitomycin or radiation) or aneugenic agents (vinblastine). MATERIALS AND METHODS: Blood samples from 12 healthy donors were exposed to 137Cs at seven doses from 0.1-4 Gy and cultured for 72 h. Cytochalasin B was added at 46 h of culture. The exposure of chemical agents (mitomycin or vinblastine) was performed after 48 h of culture for 3 h. Cytochalasin B was added after treatment and slides were prepared 24 h after. MN was semi-automatically scored following TC staining. Nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) were assessed in a human cell line after TC staining. RESULTS: The introduction TC staining to the scoring of MN not only renders MN scoring more efficient and robust, but also permits discrimination between exposure to clastogenic (MN with only telomere signals) and aneugenic agents (MN with both TC signals). The resulting improvement of MN detection led to an increase in the sensitivity of the CBMN assay following low-dose radiation exposure (0.3 versus 0.1 Gy). Hyperradiosensitivity phenomenon was observed after low dose exposure. A dose-response curve was obtained for up to 4 Gy. In addition, TC staining permits assessment of the nature of NPBs and NBUDs as biomarkers for genotoxicity and chromosomal instability. CONCLUSION: These approaches can be potentially used to follow-up populations exposed to genotoxic agents and assess cancer risk.


Asunto(s)
Centrómero/efectos de los fármacos , Daño del ADN/efectos de los fármacos , Pruebas de Mutagenicidad , Telómero/efectos de los fármacos , Aneugénicos/farmacología , Centrómero/genética , Citocinesis/efectos de los fármacos , Citocinesis/genética , Daño del ADN/genética , Humanos , Linfocitos/efectos de los fármacos , Micronúcleos con Defecto Cromosómico/efectos de los fármacos , Pruebas de Micronúcleos , Mutágenos/toxicidad , Medición de Riesgo , Telómero/genética
9.
Mutat Res ; 850-851: 503150, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32247559

RESUMEN

Extremely low frequency electromagnetic fields have been classified as a possible human carcinogen by the International Agency for Research on Cancer and this has raised some concern about its health effects on employees extensively exposed to these fields at thermal power plants. In this study, the effect of using vitamin E and C supplements have been examined on employees working at a thermal power plant. In this randomized controlled, double-blind clinical trial, 81 employees from different parts of the thermal power plant were enrolled between July and November 2017, and divided into four groups: Group 1 received vitamin E (400 units/day), Group 2: vitamin C (1000 mg/day), Group 3: vitamin E + C and Group 4: no intervention. DNA damage was measured in peripheral blood lymphocytes using comet assay and apoptosis, using flow cytometry. Based on the results, tail intensity and tail length in the vitamin E group, and all comet assay indices in the vitamin E + C and vitamin C groups (except DNA damage index) significantly decreased after the intervention, while the comet assay indices did not change significantly in the control group. None of the flow cytometry indices including early apoptosis, late apoptosis and necrosis changed after intervention in either group. The use of antioxidant vitamins such as E and C, can increase the activity of the non-enzymatic antioxidant defense system, and protect DNA from damage caused by exposure to extremely low frequency magnetic fields. But, taking these vitamins has no effect on apoptosis. It seems that consumption of vitamin E affected all investigated comet assay indices and can be probably considered as the best intervention.


Asunto(s)
Antioxidantes/administración & dosificación , Ácido Ascórbico/administración & dosificación , Daño del ADN/efectos de los fármacos , Vitamina E/administración & dosificación , Adulto , Apoptosis/efectos de los fármacos , Daño del ADN/genética , Método Doble Ciego , Citometría de Flujo , Humanos , Irán , Linfocitos/efectos de los fármacos , Linfocitos/patología , Campos Magnéticos/efectos adversos , Masculino , Centrales Eléctricas
10.
Medicine (Baltimore) ; 99(17): e19877, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32332656

RESUMEN

This study explored the prognostic value of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in rectal cancer patients receiving neoadjuvant concurrent chemoradiotherapy (CCRT).Between January 2006 and December 2016, 184 patients with newly-diagnosed rectal cancer receiving neoadjuvant CCRT were enrolled. Risk of overall survival (OS) and disease-free survival (DFS) were calculated using the Kaplan-Meier method and Cox proportional hazard models. Stratified survival analyses were also performed between post-neoadjuvant pathological (yp) stage.The mean follow-up time was 72.73 ±â€Š36.82 months. High- and low-NLR patients differed significantly in both 5-year DFS (P = .026) and OS (P = .016). High- and low-PLR patients differed significantly in 5-year DFS (P = .011) but not OS (P = .185). Multivariate analyses revealed worse 5-year DFS (adjusted HR [aHR] = 2.8; 95% CI: 1.473-5.41; P = .002) and 5-year OS (aHR = 1.871; 95%CI: 1.029-3.4; P = .04) in the high-NLR group after adjusting for covariates. After adjustments, the high-PLR group had inferior 5-year DFS (aHR = 2.274; 95%CI: 1.473-5.419; P = .038) but not 5-year OS (aHR = 1.156; 95%CI: 0.650-2.056; P = .622). Further stratified analysis indicated that yp stage II and III patients with high NLR had worse 5-year DFS (aHR = 2.334; 95% CI: 1.158-4.725; P = .018) and OS (aHR = 2.226; 95% CI: 1.165-4.251; P = .015). Additionally, yp stage II and III patients with high PLR had inferior 5-year DFS (aHR = 2.012; 95% CI: 1.049-3.861; P = .036).Pre-CCRT NLR and PLR are independent prognostic factors for rectal cancer patients and could be used as a potential biomarker to identify high-risk patients for more intense treatment and care.


Asunto(s)
Linfocitos/clasificación , Neutrófilos/clasificación , Valor Predictivo de las Pruebas , Neoplasias del Recto/mortalidad , Anciano , Quimioradioterapia/efectos adversos , Quimioradioterapia/métodos , Femenino , Humanos , Estimación de Kaplan-Meier , Recuento de Leucocitos/métodos , Recuento de Leucocitos/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Modelos de Riesgos Proporcionales , Neoplasias del Recto/sangre , Neoplasias del Recto/complicaciones , Estudios Retrospectivos
11.
Medicine (Baltimore) ; 99(14): e19638, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32243392

RESUMEN

BACKGROUND: This study aimed to systematically assess the prognostic value of lymphocyte monocyte ratio (LMR) in patients with ovarian cancer through performing a meta-analysis. METHODS: Web of Science, PubMed, EMBASE, Cochrane Library, and China National Knowledge Infrastructure databases were searched for potentially eligible studies. The baseline characteristics and relevant data were extracted. Hazard ratios with 95% confidence intervals (CIs) were combined to assess the prognostic value of LMR in patients with ovarian cancer. RESULTS: Nine studies enrolling 2809 patients were included. The pooled hazard ratios of lower LMR for overall survival and progression free survival in patients with ovarian cancer were 1.71 (95% CI, 1.40-2.09) and 1.68 (95% CI, 1.49-1.88), respectively. Subgroup analysis and sensitivity analysis were also performed. No significant publication bias was found. CONCLUSION: Our results suggested that lower LMR was associated with poorer overall survival and progression free survival in patients with ovarian cancer. The findings may assist prognosis evaluation and future research on therapies based on modulating host immune response in ovarian cancer.


Asunto(s)
Linfocitos/metabolismo , Monocitos/metabolismo , Neoplasias Ováricas/mortalidad , Femenino , Humanos , Recuento de Leucocitos , Persona de Mediana Edad , Neoplasias Ováricas/sangre , Valor Predictivo de las Pruebas , Pronóstico , Supervivencia sin Progresión , Modelos de Riesgos Proporcionales , Tasa de Supervivencia
12.
Infect Dis Poverty ; 9(1): 45, 2020 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-32345362

RESUMEN

BACKGROUND: Since its discovery in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 2 180 000 people worldwide and has caused more than 150 000 deaths as of April 16, 2020. SARS-CoV-2, which is the virus causing coronavirus disease 2019 (COVID-19), uses the angiotensin-converting enzyme 2 (ACE2) as a cell receptor to invade human cells. Thus, ACE2 is the key to understanding the mechanism of SARS-CoV-2 infection. This study is to investigate the ACE2 expression in various human tissues in order to provide insights into the mechanism of SARS-CoV-2 infection. METHODS: We compared ACE2 expression levels across 31 normal human tissues between males and females and between younger (ages ≤ 49 years) and older (ages > 49 years) persons using two-sided Student's t test. We also investigated the correlations between ACE2 expression and immune signatures in various tissues using Pearson's correlation test. RESULTS: ACE2 expression levels were the highest in the small intestine, testis, kidneys, heart, thyroid, and adipose tissue, and were the lowest in the blood, spleen, bone marrow, brain, blood vessels, and muscle. ACE2 showed medium expression levels in the lungs, colon, liver, bladder, and adrenal gland. ACE2 was not differentially expressed between males and females or between younger and older persons in any tissue. In the skin, digestive system, brain, and blood vessels, ACE2 expression levels were positively associated with immune signatures in both males and females. In the thyroid and lungs, ACE2 expression levels were positively and negatively associated with immune signatures in males and females, respectively, and in the lungs they had a positive and a negative correlation in the older and younger groups, respectively. CONCLUSIONS: Our data indicate that SARS-CoV-2 may infect other tissues aside from the lungs and infect persons with different sexes, ages, and races equally. The different host immune responses to SARS-CoV-2 infection may partially explain why males and females, young and old persons infected with this virus have markedly distinct disease severity. This study provides new insights into the role of ACE2 in the SARS-CoV-2 pandemic.


Asunto(s)
Betacoronavirus , Peptidil-Dipeptidasa A/genética , Receptores Virales/genética , Adulto , Factores de Edad , Anciano , Encéfalo/enzimología , Sistema Cardiovascular/enzimología , Sistema Cardiovascular/inmunología , Sistema Digestivo/enzimología , Sistema Digestivo/inmunología , Glándulas Endocrinas/enzimología , Glándulas Endocrinas/inmunología , Femenino , Perfilación de la Expresión Génica , Humanos , Sistema Inmunológico/enzimología , Interferones/inmunología , Pulmón/enzimología , Pulmón/inmunología , Linfocitos/inmunología , Masculino , Persona de Mediana Edad , Especificidad de Órganos , Peptidil-Dipeptidasa A/sangre , RNA-Seq , Receptores Virales/sangre , Factores Sexuales , Sistema Urogenital/enzimología
13.
Mutat Res ; 852: 503163, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32265037

RESUMEN

Cardiac catheterization procedures are performed on about 20,000 children with congenital heart disease (CHD) annually in China. The procedure, which involves exposure to ionizing radiation, causes DNA damage and may lead to increased cancer risk. We have studied chromosomal aberrations (CA) in peripheral lymphocytes of CHD children. CA frequencies were assessed in an interventional group of 70 children who underwent cardiac catheterization and a control group of 51 children receiving open-heart surgery. Total CA and all chromosome-type aberrations were higher in the exposed children than in the control group. With respect to the type of septal defect, the translocation frequency was higher in patients with ventricular rather than atrial defects. Cardiac catheterization procedures increase CA frequencies and may also increase the risk of cancer.


Asunto(s)
Cateterismo Cardíaco/efectos adversos , Aberraciones Cromosómicas/efectos de la radiación , Cardiopatías Congénitas/cirugía , Linfocitos/efectos de la radiación , Radiación Ionizante , Adolescente , Cateterismo Cardíaco/métodos , Estudios de Casos y Controles , Niño , Preescolar , China , Femenino , Cardiopatías Congénitas/patología , Humanos , Linfocitos/inmunología , Masculino , Tempo Operativo , Cultivo Primario de Células , Riesgo
14.
Emerg Microbes Infect ; 9(1): 651-663, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32192415

RESUMEN

Equine parvovirus-hepatitis (EqPV-H) has recently been associated with cases of Theiler's disease, a form of fulminant hepatic necrosis in horses. To assess whether EqPV-H is the cause of Theiler's disease, we first demonstrated hepatotropism by PCR on tissues from acutely infected horses. We then experimentally inoculated horses with EqPV-H and 8 of 10 horses developed hepatitis. One horse showed clinical signs of liver failure. The onset of hepatitis was temporally associated with seroconversion and a decline in viremia. Liver histology and in situ hybridization showed lymphocytic infiltrates and necrotic EqPV-H-infected hepatocytes. We next investigated potential modes of transmission. Iatrogenic transmission via allogeneic stem cell therapy for orthopedic injuries was previously suggested in a case series of Theiler's disease, and was demonstrated here for the first time. Vertical transmission and mechanical vectoring by horse fly bites could not be demonstrated in this study, potentially due to limited sample size. We found EqPV-H shedding in oral and nasal secretions, and in feces. Importantly, we could demonstrate EqPV-H transmission via oral inoculation with viremic serum. Together, our findings provide additional information that EqPV-H is the likely cause of Theiler's disease and that transmission of EqPV-H occurs via both iatrogenic and natural routes.


Asunto(s)
Hepatitis Viral Animal/virología , Enfermedades de los Caballos/virología , Hígado/virología , Infecciones por Parvoviridae/veterinaria , Parvovirus/fisiología , Animales , Dípteros/virología , Heces/virología , Femenino , Hepatitis Viral Animal/patología , Hepatitis Viral Animal/transmisión , Hepatocitos/patología , Hepatocitos/virología , Enfermedades de los Caballos/patología , Enfermedades de los Caballos/transmisión , Caballos , Transmisión Vertical de Enfermedad Infecciosa , Insectos Vectores/virología , Hígado/patología , Linfocitos , Masculino , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/virología , Boca/virología , Necrosis , Infecciones por Parvoviridae/patología , Infecciones por Parvoviridae/transmisión , Infecciones por Parvoviridae/virología , Parvovirus/aislamiento & purificación , Parvovirus/patogenicidad , Tropismo Viral , Viremia , Esparcimiento de Virus
15.
Bratisl Lek Listy ; 121(3): 206-210, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32115978

RESUMEN

OBJECTIVES: The aim of the present study was to evaluate the relationship between exercise capacity and n/lymphocyte ratio (NLR) in patients enrolled in a cardiopulmonary rehabilitation program. BACKGROUND: NLR has recently been used as a potential marker to determine inflammation in cardiac and non-cardiac diseases. METHODS: In this retrospective study, an exercise test and six-minute walking test (6MWT) were carried out in 23 patients with coronary artery disease and 28 patients with pulmonary disease before cardiopulmonary rehabilitation program, and routine hemogram test results were evaluated. RESULTS: The result of 6MWT test distance was 333.43 ± 86.58 m in the cardiac rehabilitation group and 348.46 ± 81.37 m in the pulmonary rehabilitation group. There was a negative correlation between 6MWT and NLR in the cardiac rehabilitation group (p < 0.05). As NLR increased, the MET value and duration of exercise decreased in the pulmonary rehabilitation group (p < 0.05). CONCLUSION: NLR could be used as a predictor to evaluate the exercise capacity in patients to be enrolled in cardiopulmonary rehabilitation program (Tab. 3, Ref. 30) Keywords: exercise capacity, neutrophil/lymphocyte ratio, rehabilitation.


Asunto(s)
Rehabilitación Cardiaca , Tolerancia al Ejercicio , Linfocitos , Neutrófilos , Humanos , Estudios Retrospectivos , Caminata
16.
Medicine (Baltimore) ; 99(10): e19405, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32150090

RESUMEN

Advanced gastric cancer has a poor prognosis because of advanced gastric cancer is prone to metastasis. It is urgent for us to find an indicator to predict the prognosis of gastric cancer in a timely fashion. Research has revealed that inflammation has an important role in predicting survival in some cancers. The purpose of this study was to evaluate the significance of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) on the prognosis of metastatic gastric cancer (GC).This was a retrospective review of 110 patients were at presentation diagnosed with stage IV metastatic GC and all patients received palliative chemotherapy between January 2012 and January 2016 at the Affiliated Hospital of Qingdao University. Pretreatment NLR and PLR, as well as clinicopathological characteristics were collected. Patients were divided into high and low groups according to the cutoff values for NLR and PLR. The Kaplan-Meier method was applied to estimate the overall survival (OS) and the Cox proportional hazards model to evaluate the related risk factors for OS. All tests were 2-tailed and a P < .05 was considered to indicate a statistically significant difference.One hundred ten patients were enrolled. Eighty-four patients were men, 24 patients were women, 61 patients were ≥65 years of age, and 49 patients were <65 years of age. The Eastern Cooperative Oncology Group (ECOG) score of most patients (n = 107) ranged from 0 to 1. Ten patients were human epidermal growth factor receptor 2 (HER2)-positive. Seventy-one patients presented with an elevated carcinoembryonic antigen (CEA) level and 49 patients had an elevated Carcinoembryonic 199 (CA-199) level. Fifty-two patients received first-line chemotherapy only. Nineteen patients received third-line or greater chemotherapy. One hundred patients chose dual drug chemotherapy. The median duration of follow-up was 11.6 months. Based on the receiver operating characteristic (ROC) curve, the optimal cut-off value for NLR and PLR was 2.48 and 143.39. Patients with high NLR and high PLR had poor overall survival compared with those who had low NLR and low PLR (P < .001 and P = .013, respectively). In univariate analysis, old age (P = .013), liver metastasis (P = .001), >1 metastatic sites (P = .028), higher NLR (P = .000), and higher PLR (P = .014) were identified as poor prognostic factors associated with OS. Our multivariate analysis had indicated that high NLR (hazard ratio [HR]: 1.617, 95% CI: 1.032-2.525, P = .036) and peritoneal metastasis (HR: 1.547, 95% CI:1.009-2.454, P = .045) was independent prognostic factors for overall survival; however, the PLR was not shown to be an independent prognostic factor.Our study suggested that the pretreatment NLR can be used as significant prognosis biomarker in metastatic gastric cancer patients receiving palliative chemotherapy.


Asunto(s)
Neoplasias Gástricas/mortalidad , Anciano , Biomarcadores de Tumor/sangre , Plaquetas/citología , China , Femenino , Humanos , Linfocitos/citología , Masculino , Metástasis de la Neoplasia , Neutrófilos/citología , Valor Predictivo de las Pruebas , Pronóstico , Modelos de Riesgos Proporcionales , Estudios Retrospectivos , Neoplasias Gástricas/sangre , Neoplasias Gástricas/patología , Análisis de Supervivencia
17.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(4): 483-485, 2020 Apr 10.
Artículo en Chino | MEDLINE | ID: mdl-32219843

RESUMEN

OBJECTIVE: To explore the genetic basis for a female with a peripheral lymphocyte karyotype of trisomy 18 but normal intelligence. METHODS: G-banding karyotype analysis, fluorescence in situ hybridization (FISH) and single nucleotide polymorphism microarray (SNP array) were employed to analyze the peripheral blood sample and buccal cells from the patient. RESULTS: Chromosomal karyotyping, SNP array and FISH analysis of the patient's peripheral blood all suggested 47,XX,+18. Interphase FISH analysis of buccal cells, however, revealed presence of 45,X and low percentage of trisomy 18 and monosomy 18. CONCLUSION: The clinical manifestation of germ layer chromosomal mosaicism is complex. The impact of the genetic disorder on the individual will depend on the structure and function derived from the affected germ layer.


Asunto(s)
Cariotipificación , Síndrome de la Trisomía 18/genética , Femenino , Humanos , Hibridación Fluorescente in Situ , Inteligencia , Cariotipo , Linfocitos , Mosaicismo , Mucosa Bucal , Polimorfismo de Nucleótido Simple
18.
Anticancer Res ; 40(3): 1503-1512, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32132050

RESUMEN

AIM: We examined whether the perioperative systemic inflammation score (SIS), which describes systemic inflammation and/or malnutrition, affected the tumor recurrence and survival in advanced gastric cancer patients. PATIENTS AND METHODS: The study retrospectively analyzed 160 patients with stage II/III gastric cancer who underwent curative resection at the Kanagawa Cancer Center. The SIS was evaluated before surgery, one week after surgery and one month after surgery, as determined by the serum albumin level (cut-off value=4.0 g/dl) and lymphocyte-to-monocyte ratio (cut-off value=4.44). RESULTS: A high SIS at one month after surgery was identified as an independent predictor for overall survival [hazard ratio (HR)=2.143, p=0.020] and showed a marginal significance for the relapse-free survival (HR=1.814, p=0.053) in multivariate analyses. CONCLUSION: The SIS at one month after surgery is a useful biomarker for predicting the long-term outcome in patients with advanced gastric cancer.


Asunto(s)
Inflamación/patología , Recurrencia Local de Neoplasia/patología , Neoplasias Gástricas/patología , Adulto , Anciano , Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Capecitabina/administración & dosificación , Quimioterapia Adyuvante , Humanos , Inflamación/sangre , Linfocitos/patología , Persona de Mediana Edad , Monocitos/patología , Estadificación de Neoplasias , Oxaloacetatos/administración & dosificación , Periodo Perioperatorio , Pronóstico , Estudios Retrospectivos , Neoplasias Gástricas/sangre , Neoplasias Gástricas/tratamiento farmacológico , Neoplasias Gástricas/cirugía , Análisis de Supervivencia , Adulto Joven
19.
Science ; 367(6482): 1091-1097, 2020 03 06.
Artículo en Inglés | MEDLINE | ID: mdl-32139536

RESUMEN

Many disease pathologies can be understood through the elucidation of localized biomolecular networks, or microenvironments. To this end, enzymatic proximity labeling platforms are broadly applied for mapping the wider spatial relationships in subcellular architectures. However, technologies that can map microenvironments with higher precision have long been sought. Here, we describe a microenvironment-mapping platform that exploits photocatalytic carbene generation to selectively identify protein-protein interactions on cell membranes, an approach we term MicroMap (µMap). By using a photocatalyst-antibody conjugate to spatially localize carbene generation, we demonstrate selective labeling of antibody binding targets and their microenvironment protein neighbors. This technique identified the constituent proteins of the programmed-death ligand 1 (PD-L1) microenvironment in live lymphocytes and selectively labeled within an immunosynaptic junction.


Asunto(s)
Antígeno B7-H1/metabolismo , Membrana Celular/metabolismo , Microambiente Celular , Linfocitos/metabolismo , Mapeo de Interacción de Proteínas/métodos , Mapas de Interacción de Proteínas , Catálisis , Membrana Celular/efectos de la radiación , Transferencia de Energía , Humanos , Células Jurkat , Linfocitos/efectos de la radiación , Metano/análogos & derivados , Metano/química , Metano/efectos de la radiación , Procesos Fotoquímicos , Rayos Ultravioleta
20.
J Clin Invest ; 130(5): 2202-2205, 2020 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-32217834

RESUMEN

The pandemic coronavirus infectious disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is rapidly spreading across the globe. In this issue of the JCI, Chen and colleagues compared the clinical and immunological characteristics between moderate and severe COVID-19. The authors found that respiratory distress on admission is associated with unfavorable outcomes. Increased cytokine levels (IL-6, IL-10, and TNF-α), lymphopenia (in CD4+ and CD8+ T cells), and decreased IFN-γ expression in CD4+ T cells are associated with severe COVID-19. Overall, this study characterized the cytokine storm in severe COVID-19 and provides insights into immune therapeutics and vaccine design.


Asunto(s)
Betacoronavirus/inmunología , Infecciones por Coronavirus/inmunología , Citocinas/inmunología , Neumonía Viral/inmunología , Factores de Edad , Infecciones por Coronavirus/complicaciones , Infecciones por Coronavirus/fisiopatología , Infecciones por Coronavirus/virología , Citocinas/sangre , Progresión de la Enfermedad , Humanos , Linfocitos/citología , Linfocitos/inmunología , Pandemias , Neumonía Viral/complicaciones , Neumonía Viral/fisiopatología , Neumonía Viral/virología , Síndrome de Dificultad Respiratoria del Adulto/etiología , Índice de Severidad de la Enfermedad
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