Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 14.995
Filtrar
1.
Int Heart J ; 61(1): 160-168, 2020 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-31956132

RESUMEN

Pulmonary arterial hypertension (PAH) is a serious and fatal cardiovascular disorder characterized by increased pulmonary vascular resistance and progressive pulmonary vascular remodeling. The underlying pathological mechanisms of PAH are multi-factorial and multi-cellular. Alginate oligosaccharide (AOS), which is produced by depolymerizing alginate, shows better pharmacological activities and beneficial effects. The present study was undertaken to investigate the effects and potential mechanisms of AOS-mediated alleviation of pulmonary hypertension. Pulmonary hypertension was induced in Sprague-Dawley rats by a single intraperitoneal injection of monocrotaline (MCT; 60 mg/kg). Five weeks after the injection of MCT, AOS (5, 10, and 20 mg·kg-1·d-1) was injected intraperitoneally for another three weeks. The results showed that AOS prevented the development of MCT-induced pulmonary hypertension and right ventricular hypertrophy in a dose-dependent manner. AOS treatment also prevented MCT-induced pulmonary vascular remodeling via inhibition of the TGF-ß1/p-Smad2 signaling pathway. Furthermore, AOS treatment downregulated the expression of malondialdehyde, nicotinamide adenine dinucleotide phosphate oxidase, and pro-inflammatory cytokines, decreased macrophage infiltration, and upregulated the expression of anti-inflammatory cytokines. These findings indicate that AOS exerts anti-oxidative and anti-inflammatory effects in pulmonary arteries, which may contribute to the alleviation of pulmonary hypertension and pulmonary vascular remodeling.


Asunto(s)
Alginatos/administración & dosificación , Antiinflamatorios/administración & dosificación , Antioxidantes/administración & dosificación , Hipertrofia Ventricular Derecha/tratamiento farmacológico , Monocrotalina/efectos adversos , /tratamiento farmacológico , Alginatos/farmacología , Animales , Antiinflamatorios/farmacología , Antioxidantes/farmacología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Hipertrofia Ventricular Derecha/inducido químicamente , Hipertrofia Ventricular Derecha/metabolismo , Inyecciones Intraperitoneales , Masculino , Malondialdehído/metabolismo , /metabolismo , Distribución Aleatoria , Ratas , Remodelación Vascular/efectos de los fármacos
2.
J Sci Food Agric ; 100(3): 1099-1105, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31667840

RESUMEN

BACKGROUND: γ-Aminobutyric acid (GABA) is a non-protein amino acid with several functions in the human body. Although freeze-thawing could effectively accumulate GABA in soybean sprouts, the mechanism has not been revealed. The mechanism by which freeze-thawing enhances GABA accumulation in germinated soybean was revealed by evaluating GABA content, the activity of related synthesis enzymes, and the microstructure of the tissues and cells of sprouts. The germinated soybeans were treated at different temperatures (from -196 °C to 25 °C) for 12 h and then thawed at 25 °C for 6 h. RESULTS: The results showed that GABA content in frozen soybean sprouts did not change significantly before thawing. After thawing, the GABA content of sprouts increased by 83.9% and 82.9% when treated by liquid nitrogen flash freeze at - 80 °C for 12 h compared with the control (4 °C treatment for 12 h). The results indicated that GABA formation mainly occurred during thawing. However, glutamate decarboxylase (GAD), diamine oxidase (DAO), and aminoaldehyde dehydrogenase (AMADH) activity decreased during thawing. Based on the malonaldehyde (MDA) content and microstructure of sprouts, it was suggested that freezing at lower temperatures (< -20 °C) maintained the integrity of the cell structure, while the tissues and cell membranes were broken during thawing. CONCLUSION: These results could provide evidence for the hypothesis that GABA formation resulted from full contact between enzymes and substrates during thawing, rather than the contribution of higher enzyme activity. © 2019 Society of Chemical Industry.


Asunto(s)
Producción de Cultivos/métodos , Semillas/química , Soja/crecimiento & desarrollo , Ácido gamma-Aminobutírico/metabolismo , Amina Oxidasa (conteniendo Cobre)/análisis , Amina Oxidasa (conteniendo Cobre)/metabolismo , Frío , Congelación , Germinación , Glutamato Descarboxilasa/análisis , Glutamato Descarboxilasa/metabolismo , Malondialdehído/análisis , Malondialdehído/metabolismo , Proteínas de Plantas/análisis , Proteínas de Plantas/metabolismo , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Soja/química , Soja/metabolismo , Ácido gamma-Aminobutírico/análisis
3.
J Sci Food Agric ; 100(3): 961-968, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31591725

RESUMEN

BACKGROUND: Among the challenges for postharvest researchers is that of understanding the physiological and biochemical pathways associated with postharvest fruit decay. Fruit senescence directly affects sensorial and nutritional quality during postharvest life. It has been clarified that reactive oxygen species and oxidative damage are responsible for fruit senescence. Some cultivars of yellow-fleshed kiwifruit can be stored for a short period compared with green-fleshed kiwifruit. Postharvest performance is affected by the physiological state of the fruit at harvest, associated with its postharvest management. Among several postharvest applications, ozone treatment is considered as a cost-effective and eco-friendly food-processing technology to preserve the fruits' quality during cold storage. In this study, we investigated the influence of ozone, after gradual cooling treatment, on the antioxidant defense system in Actinidia chinensis, 'Soreli'. RESULTS: Bioactive compound content decreased during cold storage, and ozone treatment enhanced the activities of superoxide dismutase and catalase during cold storage. This treatment preserved membrane integrity by inhibiting lipoxygenase activity and malondialdehyde accumulation. A multivariate statistical approach, using principal component analysis, provided the global response to the effect of ozone postharvest treatment during cold storage in kiwifruit 'Soreli'. CONCLUSION: Ozone treatment improves the efficiency of antioxidative system and storability of 'Soreli' kiwifruits. © 2019 Society of Chemical Industry.


Asunto(s)
Actinidia/química , Antioxidantes/análisis , Conservación de Alimentos/métodos , Frutas/efectos de los fármacos , Ozono/farmacología , Actinidia/efectos de los fármacos , Actinidia/crecimiento & desarrollo , Actinidia/metabolismo , Antioxidantes/metabolismo , Color , Conservación de Alimentos/instrumentación , Frutas/química , Frutas/crecimiento & desarrollo , Frutas/metabolismo , Malondialdehído/metabolismo , Proteínas de Plantas/metabolismo , Superóxido Dismutasa/metabolismo
4.
Life Sci ; 241: 117168, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31838133

RESUMEN

AIM: Immunosenescence is the decline of the host immune system due to aging, resulting in various complications. The splenic lymphoid nodule is the pivotal compartment involved in immunosenescence. In this study, we investigated the important changes in the splenic immune cell populations of aged rats (18-24 months) in comparison with young rats (3-5 months). MATERIALS AND METHODS: We, also, studied the effects of aging on the activities of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) levels in spleen of both groups, besides the changes of the splenic architecture. Furthermore, immunohistochemical staining was performed to detect the aging effects in T cells, B cells, macrophages, granulocytes, mast cells, proliferating cells, apoptotic cells, and cells positive for interleukin-1ß (IL-1ß), interleukin-6 (IL-6), and Toll-like receptor 4 (TLR4). KEY FINDINGS: The aged rats had significantly lower spleen/body weight ratios and smaller splenic nodules, indicating a decline in general immunity in them. With aging, T-SOD activities were decreased, while MDA levels were increased, exhibiting that oxidative stress increases in spleens. In addition, the aged group also had significantly fewer T and B cells, macrophages, granulocytes, IL-6 and TLR4 immuno-positive cells, and proliferating cells in the periarterial lymphatic sheaths, marginal zone, and lymphoid follicles compared with the young group. On the other hand, the number of mast cells and apoptotic cells was significantly increased with age. Therefore, we can conclude that cellular immunity and humoral immunity were crumpled with age.


Asunto(s)
Linfocitos B/inmunología , Inmunidad Celular/inmunología , Inmunosenescencia/inmunología , Estrés Oxidativo/inmunología , Bazo/inmunología , Linfocitos T/inmunología , Animales , Linfocitos B/metabolismo , Linfocitos B/patología , Células Cultivadas , Masculino , Malondialdehído/metabolismo , Ratas , Ratas Wistar , Bazo/metabolismo , Bazo/patología , Superóxido Dismutasa/metabolismo , Linfocitos T/metabolismo , Linfocitos T/patología
5.
Chemosphere ; 239: 124668, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31494325

RESUMEN

Nitrite is one of major environmental pollutants that can impact immunological parameters in aquatic organisms. In the present study, we investigated the effects of nitrite exposure on oxidative stress, DNA damage and apoptosis in mud crab (Scylla paramamosain). Mud crab were exposed to 0, 5, 10 and 15 mg L-1 nitrite for 72 h. These data showed that acid phosphatase (ACP) and alkaline phosphatase (ALP) activity significantly decreased in treatments with various concentrations of nitrite (5, 10 and 15 mg L-1) after 24 and 48 h, while the levels of nitric oxide (NO) significantly increased in these treatments. Nitrite exposure could suppress superoxide dismutase (SOD) and catalase (CAT) activity, and increase the formation of malondialdehyde (MDA) after 48 and 72 h of exposure. In addition, nitrite exposure decreased total haemocyte counts after 48 and 72 h of exposure. Cytological damage, DNA damage and apoptosis was observed obviously at 72 h after nitrite exposure. Moreover, nitrite exposure significantly induced the mRNA levels of phosphorylated Jun N-terminal kinases (JNK), and eventually activated p53 signaling and caspase-3. These results indicated that nitrite exposure could induce oxidative stress, which further caused DNA damage and apoptosis in mud crab. Our results will be helpful to understand the mechanism of nitrite toxicity on crustaceans.


Asunto(s)
Apoptosis/efectos de los fármacos , Braquiuros/efectos de los fármacos , Daño del ADN/genética , Nitritos/toxicidad , Estrés Oxidativo/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Fosfatasa Ácida/metabolismo , Fosfatasa Alcalina/metabolismo , Animales , Braquiuros/genética , Catalasa/metabolismo , Hemocitos , Malondialdehído/metabolismo , Óxido Nítrico/metabolismo , Transducción de Señal , Superóxido Dismutasa/metabolismo
6.
Ecotoxicol Environ Saf ; 188: 109756, 2020 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-31711776

RESUMEN

Soil co-contaminated with heavy metals and organics is often difficult to remediate. In this study, pot experiments were conducted to investigate the concurrent removal of cadmium (Cd, two levels: CdL [10 mg kg-1] and CdH [50 mg kg-1]) and trichlorfon (TCF, 100 mg kg-1) from co-contaminated soil by comparing the following remediation methods: natural remediation (NR), soil inoculated with Aspergillus sydowii (AS), soil planted with Brassica juncea (BJ), and soil planted with B. juncea and inoculated with A. sydowii (BJ-AS). The physiological responses of B. juncea and soil enzyme activities after remediation were also studied. B. juncea grew well in co-contaminated soil at both Cd levels. The biomass and chlorophyll content of B. juncea in CdH soil were lower than those in CdL soil, whereas the malondialdehyde content and activities of catalase, peroxidase and superoxide dismutase of B. juncea in CdH soil were higher than those in CdL soil. Cd accumulation in B. juncea was high in CdH soil, whereas high Cd removal efficiency was observed in CdL soil. TCF could be thoroughly degraded within 35 days in NR at both Cd-level soils. AS, BJ and BJ-AS promoted TCF degradation and enhanced the activities of catalase, urease, sucrase and alkaline phosphatase in soil compared with the NR. BJ-AS showed the highest phytoextraction ratio (3.32% in CdL and 1.34% in CdH soil) and TCF degradation rate (half-life of 2.18 and 2.37 days in CdL and CdH soil, respectively). These results demonstrate that BJ-AS could effectively remove Cd and TCF from soil and is thus a feasible technology for the bioremediation of these co-contaminated soil.


Asunto(s)
Aspergillus/crecimiento & desarrollo , Cadmio/análisis , Planta de la Mostaza/crecimiento & desarrollo , Contaminantes del Suelo/análisis , Suelo/química , Triclorfón/análisis , Aspergillus/metabolismo , Biodegradación Ambiental , Biomasa , Cadmio/metabolismo , Catalasa/metabolismo , Malondialdehído/metabolismo , Planta de la Mostaza/metabolismo , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Triclorfón/metabolismo
7.
Ecotoxicol Environ Saf ; 187: 109788, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31648073

RESUMEN

The potential threats of microplastics to global health are a new problem. However, little is known about the influence of microplastics on soil organisms. Here, we investigated the effects of low-density polyethylene (LDPE, < 400 µm) on earthworms (Eisenia fetida) under different concentrations (0.1, 0.25, 0.5, 1.0, 1.5 g/kg dry) with three replicates in artificial soil. Results showed that surface damage of earthworms was observed at the concentration of 1.5 g/kg LDPE after exposure 28 days. The microplastics were ingested in a dose-response manner. Smaller sizes of LDPE microplastics were found in the casts of E. fetida, and approximately 30% of the microplastics egested (size < 100 µm) were increased compared with initial microplastics in the soil. The catalase activity and malondialdehyde content increased significantly at the concentration of 1.0 g/kg LDPE after exposure 28 days, and acetylcholine esterase was significantly stimulated at concentrations of 1.5 and 1.0 g/kg LDPE on days 21 and 28, respectively. The results of this study demonstrate the potential risk of LDPE microplastics to E. fetida and may provide a reference for the impact of microplastics on terrestrial creatures.


Asunto(s)
/toxicidad , Oligoquetos/efectos de los fármacos , Polietileno/toxicidad , Contaminantes del Suelo/toxicidad , Suelo/química , Animales , Relación Dosis-Respuesta a Droga , Malondialdehído/metabolismo , Oligoquetos/metabolismo , Tamaño de la Partícula , Polietileno/química , Contaminantes del Suelo/química
8.
Ecotoxicol Environ Saf ; 187: 109809, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31654861

RESUMEN

A theoretical non-linear combined toxicity assessment method is proposed and evaluated using Microcystis aeruginosa as the test organism. The combined toxicity of binary heavy metals was evaluated by comparing the actual inhibitory rates shown from the experiments with the theoretically calculated inhibitory rates. It was identified that the binary mixtures of Cu2++ Cd2+, Cu2++ Cr3+ and Zn2++ Cr3+ had the synergistic effects when the combined concentrations were low, but exhibited the antagonistic effects with the higher combined concentrations. Furthermore, the toxic effect of Pb2+ was not influenced by the addition of Cu2+ when combined concentration was low, but it was enhanced by Cu2+ at the high combined concentration. The binary mixtures of Zn2++ Cd2+, Pb2++ Cr3+, Pb2++ Cd2+, Pb2++ Zn2+, and Cr3++ Cd2+ always presented antagonistic effects, while the synergistic toxicity effect on M. aeruginosa was observed for the binary mixtures of Cu2++ Zn2+ regardless of combined concentration. The proposed assessment method was also validated by the antioxidant enzyme activity, which showed synergistic or antagonistic effects under different binary mixtures of heavy metals.


Asunto(s)
Metales Pesados/toxicidad , Microcystis/efectos de los fármacos , Modelos Teóricos , Antioxidantes/metabolismo , Sinergismo Farmacológico , Malondialdehído/metabolismo , Metales Pesados/química , Microcystis/enzimología , Dinámicas no Lineales , Especies Reactivas de Oxígeno/metabolismo
9.
Life Sci ; 242: 117217, 2020 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-31884094

RESUMEN

AIM: Kidney ischemia reperfusion (IR) injury is an important health problem resulting in acute kidney failure. The oxidative stress and inflammatory process are the underlying mechanisms of IR injury. It has been purposed in this study to research the possible protective effects of fraxin on kidney injury induced by IR. MATERIAL AND METHODS: 32 Sprague Dawley male rats were divided into 4 groups. The groups were organized as follows; sham, IR, IR + fraxin 10 mg/kg, and IR + 50 mg/kg fraxin groups. Some oxidant, antioxidant and inflammatory parameters were evaluated in kidney tissues removed at the end of our experimental study. KEY FINDINGS: It was detected that the oxidant and proinflammatory markers increased and antioxidant parameters decreased in IR group but the results significantly reversed in treatment groups compared to IR group. And also, 8-OHdG, NF-κB, HAVCR1 immunopositivities were at severe levels and these results attenuated in IR fraxin + 10 mg/kg, and IR + fraxin 50 mg/kg groups. SIGNIFICANCE: These presented results have shown that fraxin performed protective effects against kidney injury induced by IR.


Asunto(s)
Lesión Renal Aguda/prevención & control , Antioxidantes/uso terapéutico , Cumarinas/uso terapéutico , Daño por Reperfusión/prevención & control , Lesión Renal Aguda/etiología , Lesión Renal Aguda/patología , Animales , Modelos Animales de Enfermedad , Riñón/efectos de los fármacos , Riñón/patología , Masculino , Malondialdehído/metabolismo , Peroxidasa/metabolismo , Ratas , Ratas Sprague-Dawley , Daño por Reperfusión/complicaciones , Daño por Reperfusión/patología , Superóxido Dismutasa/metabolismo
10.
Chemosphere ; 238: 124602, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31545211

RESUMEN

Polybrominated diphenyl ethers (PBDEs) have been known to exhibit neurotoxicity in rats; however, the underlying mechanism remains unknown and there is no available intervention. In this study, we aimed to investigate the role of oxidative and nitrosative stress in the neurotoxicity in the cerebral cortex and primary neurons in rats following the BDE-153 treatment. Compared to the untreated group, BDE-153 treatment significantly induced the neurotoxic effects in rats, as manifested by the increased lactate dehydrogenase (LDH) activities and cell apoptosis rates, and the decreased neurotrophic factor contents and cholinergic enzyme activities in rats' cerebral cortices and primary neurons. When compared to the untreated group, the oxidative and nitrosative stress had occurred in the cerebral cortex or primary neurons in rats following the BDE-153 treatment, as manifested by the increments in levels of reactive oxygenspecies (ROS), malondialdehyde (MDA), nitric oxide (NO), and neuronal nitric oxide synthase (nNOS) mRNA and protein expressions, along with the decline in levels of superoxide dismutase (SOD) activity, glutathione (GSH) content, and peroxiredoxin I (Prx I) and Prx II mRNA and protein expressions. In addition, the ROS scavenger N-acetyl-l-cysteine (NAC) or NO scavenger NG-Nitro-l-arginine (L-NNA) significantly rescued the LDH leakage and cell survival, reversed the neurotrophin contents and cholinergic enzymes, mainly via regaining balance between oxidation/nitrosation and antioxidation. Overall, our findings suggested that oxidative and nitrosative stresses are involved in the neurotoxicity induced by BDE-153, and that the antioxidation is a potential targeted intervention.


Asunto(s)
Corteza Cerebral/patología , Éteres Difenilos Halogenados/toxicidad , Síndromes de Neurotoxicidad/patología , Estrés Nitrosativo/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Bifenilos Polibrominados/toxicidad , Acetilcisteína/farmacología , Animales , Antioxidantes/metabolismo , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Corteza Cerebral/efectos de los fármacos , Glutatión/metabolismo , Éteres Difenilos Halogenados/metabolismo , Masculino , Malondialdehído/metabolismo , Factores de Crecimiento Nervioso/metabolismo , Neuronas/efectos de los fármacos , Neurotrofina 3/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa de Tipo I/metabolismo , Estrés Nitrosativo/fisiología , Estrés Oxidativo/fisiología , Ratas , Ratas Sprague-Dawley , Transducción de Señal/efectos de los fármacos
11.
J Sci Food Agric ; 100(1): 92-101, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31435952

RESUMEN

BACKGROUND: Oyster polypeptides have various biofunctions, such as anti-cancer and anti-oxidative stress, but whether it has the protective effects to primary ovarian failure (POF) remains poorly understand. To address this issue, daily gavage of oyster polypeptides was performed to investigate their protective effect, basing on d-galactose-induced POF model in C57BL/6 female mice. RESULTS: Oyster polypeptides restored the irregular estrous cycles and the abnormal serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and progesterone (P) levels as well as the decreased mRNA expression level of Amh that were induced by d-galactose. The follicle development of POF mice was improved by increasing the primordial follicle ratio and decreasing the atretic follicle number after oral administration of oyster polypeptides. Moreover, in the oyster polypeptides treated mice, the total superoxide dismutase (T-SOD) activity was significantly increased, while the malondialdehyde levels were significantly decreased. The mRNA expression levels of stress-related genes (SOD2, SIRT1 and FOXO3a) were remarkably up-regulated after d-galactose induction, but the up-regulation was weakened or disappeared by the gavage of oyster polypeptides. In addition, oyster polypeptides treatment also reduced the apoptosis of the ovarian granulosa cells and down-regulated the mRNA expression levels of apoptosis-related genes (p53 and Bad but not Bcl-2). CONCLUSION: This study reveals that oyster polypeptides may protect ovary against d-galactose-induced POF by their anti-oxidative stress activity to rescue d-galactose-induced ovarian oxidative damage and therefore to prevent ovarian cells apoptosis, thereby tipping the abnormality trigged by POF to get close to the normal levels. © 2019 Society of Chemical Industry.


Asunto(s)
Ostreidae/química , Péptidos/administración & dosificación , Insuficiencia Ovárica Primaria/tratamiento farmacológico , Sustancias Protectoras/administración & dosificación , Animales , Apoptosis/efectos de los fármacos , Femenino , Galactosa/efectos adversos , Humanos , Hormona Luteinizante/metabolismo , Malondialdehído/metabolismo , Ratones , Ratones Endogámicos C57BL , Folículo Ovárico/efectos de los fármacos , Folículo Ovárico/metabolismo , Ovario/efectos de los fármacos , Ovario/metabolismo , Estrés Oxidativo/efectos de los fármacos , Insuficiencia Ovárica Primaria/inducido químicamente , Insuficiencia Ovárica Primaria/genética , Insuficiencia Ovárica Primaria/metabolismo , Progesterona/metabolismo , Sirtuina 1/genética , Sirtuina 1/metabolismo , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo
12.
Arch Oral Biol ; 109: 104529, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31574324

RESUMEN

OBJECTIVE: Periodontitis disease is a chronic inflammation, and the prevention or treatment of periodontal disease is important for improving oral health and averting systemic diseases.Acer tegmentosum Maxim (ATM) is a type of deciduous tree in Korea. ATM extracts have been traditionally used to treat various dieases. This study investigated the effects of ATM extract on mitigation of periodontitis in vitro and in vivo. DESIGN: The current study investigated whether extracts ofAcer tegmentosum Maxim (ATM) attenuated periodontitis induced by Porphyromonas gingivalis-derived lipopolysaccharide (LPS) in vitro and in vivo. We used a rat model of experimental periodontitis that received oral administration of 1 mg/kg P. gingivalis-derived LPS for 10 days. Periodontitis models was treated with two different dosages of ATM (30 or 100 mg/kg) during the same period of periodontal induction for histological analysis. RESULTS: The results indicated that aqueous ATM extracts effectively ameliorated ligature-induced periodontitis through of the antibacterial, anti-oxidative, and anti-inflammatory activities. CONCLUSION: These pre-clinical results suggest the need for further studies on the anti-periodontitis effect of ATM in humans. Thus, ATM could be used as a natural anti-periodontitis agent for the treatment of periodontitis.


Asunto(s)
Acer/química , Periodontitis/tratamiento farmacológico , Extractos Vegetales/farmacología , Porphyromonas gingivalis/patogenicidad , Animales , Línea Celular , Humanos , Lipopolisacáridos , Masculino , Malondialdehído/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Periodontitis/microbiología , Corteza de la Planta/química , Ratas , Ratas Sprague-Dawley , República de Corea
13.
J Sci Food Agric ; 100(2): 846-854, 2020 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-31646650

RESUMEN

BACKGROUND: Pomegranate has antioxidant, cardioprotective and anti-inflammatory properties. We designed a crossover study aimed at determining if consumption of pomegranate juice (PJ) improves lipid profile and oxidative and inflammatory biomarkers of hemodialysis patients. Forty-one hemodialysis patients were randomly assigned to one of two groups: PJ-treated group receiving 100 mL of natural PJ immediately after their dialysis session three times a week and the control group receiving the usual care. After 8 weeks, a 4-week washout period was established and then the role of the groups was exchanged. Lipid profile, blood pressure and oxidative and inflammatory biomarkers were measured before and after each sequence. RESULTS: Based on the results of intention-to-treat analysis, triglycerides were decreased in PJ condition and increased in the controls. Conversely, high-density lipoprotein cholesterol was increased in PJ and decreased in the control group. Total and low-density lipoprotein cholesterol did not significantly change in either condition. Systolic and diastolic blood pressure significantly decreased in PJ condition. Total antioxidant capacity increased in PJ condition (P < 0.001) and decreased in the controls (P < 0.001). Conversely, malondialdehyde and interleukin-6 decreased in PJ (P < 0.001) and increased in the control group (P ≤ 0.001). The changes of these biomarkers were significantly different between the two conditions. CONCLUSIONS: Eight-week PJ consumption showed beneficial effects on blood pressure, serum triglycerides, high-density lipoprotein cholesterol, oxidative stress and inflammation in hemodialysis patients. © 2019 Society of Chemical Industry.


Asunto(s)
Enfermedades Cardiovasculares/metabolismo , Jugos de Frutas y Vegetales/análisis , Insuficiencia Renal Crónica/dietoterapia , Adulto , Anciano , Presión Sanguínea , Enfermedades Cardiovasculares/inmunología , Enfermedades Cardiovasculares/fisiopatología , Enfermedades Cardiovasculares/prevención & control , Estudios Cruzados , Femenino , Humanos , Masculino , Malondialdehído/metabolismo , Persona de Mediana Edad , Estrés Oxidativo , Diálisis Renal , Insuficiencia Renal Crónica/complicaciones , Insuficiencia Renal Crónica/metabolismo , Insuficiencia Renal Crónica/terapia , Triglicéridos/metabolismo
14.
Sci Total Environ ; 698: 134106, 2020 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-31505350

RESUMEN

Chronic exposure to cadmium (Cd) can cause renal dysfunction. Studies of animals, cell cultures, and plants have found that selenium (Se) can effectively alleviate the hazard generated by Cd, but there has been little study of this in general human populations. This study recruited 313 subjects from China's Hubei Province, including 160 living in areas with high soil Cd and Se (exposure group) and 153 living in clean areas (control group). The levels of the following were detected: Cd and Se in blood (B-Cd and B-Se), urine (U-Cd and U-Se), and hair (H-Cd and H-Se); N-acetyl-ß-D-glucosaminidase (U-NAG), ß2-microglobulin (U-ß2-MG), and albumin (U-ALB) in urine; and malondialdehyde (S-MDA), superoxide dismutase (S-SOD), and glutathione peroxidase (S-GSH-Px) in serum. In addition, the interactions between Cd and Se were assessed. The median levels of B-Cd, B-Se, U-Cd, U-Se, H-Cd, H-Se, S-MDA, and S-GSH-Px of exposure group (2.60 ng/mL, 238.90 ng/mL, 3.13 µg/g Cr, 45.43 µg/g Cr, 0.06 µg/g, 0.70 µg/g, 5.22 nmol/mL, and 308.89 U, respectively) were significantly higher than of controls (0.95 ng/mL, 130.50 ng/mL, 1.08 µg/g Cr, 30.51 µg/g Cr, 0.04 µg/g, 0.49 µg/g, 4.71 nmol/mL, and 267.54 U, respectively), but there were no significant differences in U-NAG, U-ß2-MG, U-ALB, or S-SOD between the two groups. U-NAG levels were significantly negatively associated with the interaction between Cd and Se (B: -0.511, 95% CI: -0.886, -0.136). Additionally, changes in the direction of the estimated regression coefficient in the low and high H-Se groups were observed for U-Cd and S-MDA (from 0.018 to -0.090), U-Cd and S-GSH-Px (from -0.039 to 0.101). This study found that populations living in areas with high levels of soil Cd and Se did not show greater Cd-induced renal tubular and glomerular injuries than the control population, which could attribute to the protective effects of Se. The protective effects may be related to the peculiar function of Se that Se can combine with free Cd to activate the antioxidant enzyme system.


Asunto(s)
Cadmio/análisis , Exposición a Riesgos Ambientales/estadística & datos numéricos , Enfermedades Renales/epidemiología , Selenio/análisis , Contaminantes del Suelo/análisis , Acetilglucosaminidasa/metabolismo , Antioxidantes , Cadmio/metabolismo , China/epidemiología , Glutatión Peroxidasa/metabolismo , Humanos , Enfermedades Renales/metabolismo , Malondialdehído/metabolismo , Sustancias Protectoras/metabolismo , Selenio/metabolismo , Contaminantes del Suelo/metabolismo , Superóxido Dismutasa/metabolismo , Microglobulina beta-2/metabolismo
15.
Chemosphere ; 238: 124595, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31445330

RESUMEN

Earthworms are often used as test subjects in toxicological studies, due to their ubiquitousness and sensitivity to contaminant exposure. Such testing is typically conducted using Eisenia fetida as the test subject, but continued use of E. fetida (eco) toxicology is questionable. Therefore, in this study three earthworm species, Aporrectodea rosea, Aporrectodea trapezoides and E. fetida, were exposed to lethal and sublethal concentrations of cadmium (Cd) and lead (Pb) nitrate in artificial soil for 7, 14 and 28 days. A biomarker of genotoxicity (TUNEL assay), biochemical markers [malondialdehyde (MDA) and total antioxidant capacity (TAC)], weight loss, lethal toxicity (LC50) and subcellular partitioning were assessed. Cadmium and Pb caused significant inhibition in TAC and growth and significant increases in DNA damage and lipid peroxidation in the earthworms. Acute toxicity rank (14 days) for both Cd and Pb were E. fetida > A. trapezoides > A. rosea. Subcellular partitioning of Cd and Pb in the earthworms were cytosol > debris > granules and debris > granules > cytosol, respectively. Comparison of biomarker responses between study species showed that E. fetida proved to be less susceptible to Cd and Pb exposure than A. rosea and A. trapezoides. Therefore, this study confirms that A. rosea and A. trapezoides are more suitable as subjects than E. fetida for the soil toxicity tests, because of both their greater susceptibility to toxicants and in their abundance in the field.


Asunto(s)
Cadmio/toxicidad , Plomo/toxicidad , Oligoquetos/metabolismo , Contaminantes del Suelo/toxicidad , Animales , Biomarcadores/metabolismo , Daño del ADN/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Malondialdehído/metabolismo , Oligoquetos/clasificación , Suelo/química , Contaminantes del Suelo/análisis , Pruebas de Toxicidad
16.
Chemosphere ; 239: 124523, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31499308

RESUMEN

Arsenic (As) is a toxic metalloid that severely hampers plant growth and also poses health risks for humans through the food chain. Although nitric oxide (NO) is known to improve plant resistance to multiple stresses including metal toxicity, little is known about its role in the As tolerance of hyperaccumulator plants. This study investigates the role of the exogenously applied NO donor, sodium nitroprusside (SNP), in improving the As tolerance of Isatis cappadocica, which has been reported to hyperaccumulate As. Exposure to toxic As concentrations significantly increases NO production and damages the cell membrane, as indicated by increased hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations, thereby reducing plant growth. However, the addition of SNP improves growth and alleviates As-induced oxidative stress by enhancing the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione S-transferase (GST), glutathione (GSH), as well as proline and thiol concentrations, thereby confirming the beneficial role played by NO in increasing As stress tolerance. Furthermore, the As-induced decrease in growth and the increase in oxidative stress were more marked in the presence of bovine hemoglobin (Hb; a NO scavenger) and N(G)-nitro-l-arginine methyl ester (l-NAME; a NO synthase inhibitor), thus demonstrating the protective role of NO against As toxicity. The reduction in NO concentrations by l-NAME suggests that NOS-like activity is involved in the generation of NO in response to As in I. cappadocica.


Asunto(s)
Antioxidantes/metabolismo , Arsénico/toxicidad , Isatis/efectos de los fármacos , Óxido Nítrico/metabolismo , Nitroprusiato/farmacología , Animales , Ascorbato Peroxidasas/metabolismo , Bovinos , Membrana Celular/patología , Glutatión/metabolismo , Glutatión Reductasa/metabolismo , Peróxido de Hidrógeno/metabolismo , Isatis/metabolismo , Malondialdehído/metabolismo , Donantes de Óxido Nítrico , Estrés Oxidativo/efectos de los fármacos , Superóxido Dismutasa/metabolismo
17.
Food Chem ; 309: 125791, 2020 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-31699554

RESUMEN

The aim of this study was to investigate the effect of phosphorylation on the antioxidant activity of (1 → 3)-ß-d-glucan from yeast cell wall. Alkali-insoluble (1 → 3)-ß-d-glucan was extracted from yeast cell wall by an acid-base method. It was found that the purity of the sample was greatly improved after the precipitation was treated with alkali at 90 ℃ and then by acetic acid, which was about 96.5%. Phosphorylated (1 → 3)-ß-d-glucan was prepared. Infrared (IR) spectra and nuclear magnetic resonance spectra (NMR) confirmed the successful introduction of phosphate into glucan. The substitution degree of phosphate was 0.18. The phosphorylated (1 → 3)-ß-d-glucan could significantly increase SOD and CAT contents in serum, liver and brain of mice, and reduce MDA level in serum, liver and brain to a certain extent in vivo. This lays a solid foundation for the research and development of phosphorylated (1 → 3)-ß-d-glucan antioxidant.


Asunto(s)
Antioxidantes/metabolismo , beta-Glucanos/química , Animales , Antioxidantes/química , Catalasa/sangre , Catalasa/metabolismo , Pared Celular/química , Pared Celular/metabolismo , Espectroscopía de Resonancia Magnética , Malondialdehído/sangre , Malondialdehído/metabolismo , Ratones , Fosforilación , Saccharomyces cerevisiae/metabolismo , Espectrofotometría Infrarroja , Superóxido Dismutasa/sangre , Superóxido Dismutasa/metabolismo
18.
Artículo en Inglés | MEDLINE | ID: mdl-31669665

RESUMEN

In recent years, n-butanol has growing use in many areas, including the food industry. In this study, acute toxic effects of n-butanol to zebrafish (Danio rerio) larvae by applying different concentrations (10, 50, 250, 500, 750, 1000 and 1250 mg/L) to embryos were evaluated. For this purpose the data of oxidative stress, antioxidant - acetyl cholinesterase enzyme activities, malondialdehyde level and apoptosis were taken into consideration. At the end of the 96 h, antioxidant (Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)) and acetylcholinesterase (AChE) enzyme activities were decreased, however lipid peroxidation level, apoptotic cells, and reactive oxygen species increased (p < .05). As a result, it has been observed that high concentrations of n-butanol with its amphiphilic structure causes quite intense toxic effects in zebrafish embryos.


Asunto(s)
1-Butanol/toxicidad , Apoptosis/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Pez Cebra , Animales , Catalasa/genética , Catalasa/metabolismo , Glutatión Peroxidasa/genética , Glutatión Peroxidasa/metabolismo , Larva/efectos de los fármacos , Malondialdehído/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo
19.
Food Chem ; 309: 125608, 2020 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-31678673

RESUMEN

Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can improve wound healing of potato tubers; however, how the chemical regulates reactive oxygen species (ROS) generation and scavenging during wound healing is not completely understood. BTH at 100 mg·L-1 regulated changes in ROS generation and scavenging in healing tissues of potato tubers. A higher H2O2 content was presented in healing tissues of potato tubers, while cell membrane permeability and malondialdehyde content declined due to BTH treatment. Additionally, the activities and transcript level of enzymes related with ROS generation, including NADPH oxidase, peroxidase and polyamine oxidase, as well as enzymes involved in ROS scavenging, such as superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase, were significantly enhanced by BTH treatment. It is suggested that ROS metabolism might play a crucial role in wound healing of potato tubers mediated by BTH during postharvest.


Asunto(s)
Tubérculos de la Planta/efectos de los fármacos , Tubérculos de la Planta/metabolismo , Solanum tuberosum/efectos de los fármacos , Solanum tuberosum/metabolismo , Tiadiazoles/farmacología , Membrana Celular/efectos de los fármacos , Permeabilidad de la Membrana Celular/efectos de los fármacos , Enzimas/genética , Enzimas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Peróxido de Hidrógeno/metabolismo , Malondialdehído/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Especies Reactivas de Oxígeno/metabolismo
20.
Food Chem ; 303: 125424, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31472384

RESUMEN

The aim of our study was to investigate the effect of drop shock on physiological responses and genes expression in harvested apple fruit stored at 20 ±â€¯2 °C. Ethylene production, respiratory rate, firmness, soluble solid content, relative electrical conductivity, LOX (lipoxygenase) activity, MDA (malondialdehyde) content, variation of volatile compounds, ethylene biosynthetic genes, and ethylene receptor genes of apple fruit were examined. The results indicated that drop shock observably resulted in the increase of ethylene production, respiratory rate, soluble solid content, relative electrical conductivity, LOX activity, MDA content and gene expression levels in apples. Furthermore, drop shock significantly decreased firmness and high-intensitive drop shock stimulated the accumulation of aldehydes and esters in harvested apples. Overall, the greater impact on apple quality is the effect of larger amplitude of shock during truck transportation, which seriously reduced storage life and quality of postharvest apples.


Asunto(s)
Frutas/química , Malus/genética , Proteínas de Plantas/genética , Aldehídos/metabolismo , Fenómenos Biomecánicos , Ésteres/metabolismo , Etilenos/metabolismo , Frutas/genética , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas , Lipooxigenasa/genética , Lipooxigenasa/metabolismo , Malondialdehído/metabolismo , Malus/química , Malus/metabolismo , Proteínas de Plantas/metabolismo , Control de Calidad
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA