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1.
Biomater Sci ; 9(9): 3362-3377, 2021 May 04.
Artículo en Inglés | MEDLINE | ID: mdl-33949373

RESUMEN

Thrombosis and infection are the leading causes of blood-contacting device (BCD) failure, mainly due to the poor performance of existing biomaterials. Poly(2-hydroxyethyl methacrylate) (pHEMA) has excellent hemocompatibility but the weak mechanical properties impair its use as a bulk material for BCD. As such, pHEMA has been explored as a coating, despite the instability and difficulty of attachment to the underlying polymer compromise its success. This work describes the hydrogel composites made of pHEMA and graphene-based materials (GBM) that meet the biological and mechanical requirements for a stand-alone BCD. Five GBM differing in thickness, oxidation degree, and lateral size were incorporated in pHEMA, revealing that only oxidized-GBM can reinforce pHEMA. pHEMA/oxidized-GBM composites are cytocompatible and prevent the adhesion of endothelial cells, blood platelets, and bacteria (S. aureus), thus maintaining pHEMA's anti-adhesive properties. As a proof of concept, the thrombogenicity of the tubular prototypes of the best formulation (pHEMA/Graphene oxide (GO)) was evaluated in vivo, using a porcine arteriovenous-shunt model. pHEMA/GO conduits withstand the blood pressure and exhibit negligible adhesion of blood components, revealing better hemocompatibility than ePTFE, a commercial material for vascular access. Our findings reveal pHEMA/GO, a synthetic and off-the-shelf hydrogel, as a preeminent material for the design of blood-contacting devices that prevent thrombosis and bacterial adhesion.


Asunto(s)
Grafito , Polihidroxietil Metacrilato , Animales , Materiales Biocompatibles/farmacología , Células Endoteliales , Staphylococcus aureus , Porcinos
2.
Int J Mol Sci ; 22(7)2021 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-33807361

RESUMEN

Transforaminal lumber interbody fusion (TLIF) is the last resort to address the lumber degenerative disorders such as spondylolisthesis, causing lower back pain. The current surgical intervention for these abnormalities includes open TLIF. However, in recent years, minimally invasive TLIF (MIS-TLIF) has gained a high momentum, as it could minimize the risk of infection, blood loss, and post-operative complications pertaining to fusion surgery. Further advancement in visualizing and guiding techniques along with grafting cage and materials are continuously improving the safety and efficacy of MIS-TLIF. These assistive techniques are also playing a crucial role to increase and improve the learning curve of surgeons. However, achieving an appropriate output through TLIF still remains a challenge, which might be synergized through 3D-printing and tissue engineering-based regenerative therapy. Owing to their differentiation potential, biomaterials such as stem/progenitor cells may contribute to restructuring lost or damaged tissues during MIS-TLIF, and this therapeutic efficacy could be further supplemented by platelet-derived biomaterials, leading to improved clinical outcomes. Thus, based on the above-mentioned strategies, we have comprehensively summarized recent developments in MIS-TLIF and its possible combinatorial regenerative therapies for rapid and long-term relief.


Asunto(s)
Vértebras Lumbares/cirugía , Fusión Vertebral/métodos , Fusión Vertebral/tendencias , Materiales Biocompatibles/farmacología , Trasplante Óseo/tendencias , Cerámica , Humanos , Degeneración del Disco Intervertebral/cirugía , Región Lumbosacra/cirugía , Procedimientos Quirúrgicos Mínimamente Invasivos/métodos , Procedimientos Quirúrgicos Mínimamente Invasivos/tendencias , Medicina Regenerativa/métodos , Espondilolistesis/cirugía , Resultado del Tratamiento
3.
Nat Commun ; 12(1): 2407, 2021 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-33893308

RESUMEN

Many features of extracellular matrices, e.g., self-healing, adhesiveness, viscoelasticity, and conductivity, are associated with the intricate networks composed of many different covalent and non-covalent chemical bonds. Whereas a reductionism approach would have the limitation to fully recapitulate various biological properties with simple chemical structures, mimicking such sophisticated networks by incorporating many different functional groups in a macromolecular system is synthetically challenging. Herein, we propose a strategy of convergent synthesis of complex polymer networks to produce biomimetic electroconductive liquid metal hydrogels. Four precursors could be individually synthesized in one to two reaction steps and characterized, then assembled to form hydrogel adhesives. The convergent synthesis allows us to combine materials of different natures to generate matrices with high adhesive strength, enhanced electroconductivity, good cytocompatibility in vitro and high biocompatibility in vivo. The reversible networks exhibit self-healing and shear-thinning properties, thus allowing for 3D printing and minimally invasive injection for in vivo experiments.


Asunto(s)
Adhesivos/química , Conductividad Eléctrica , Hidrogeles/química , Metales/química , Adhesivos/síntesis química , Adhesivos/farmacología , Animales , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Bacillus subtilis/efectos de los fármacos , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Línea Celular , Proliferación Celular/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Hidrogeles/síntesis química , Hidrogeles/farmacología , Ratones , Microscopía Electrónica , Mioblastos/citología , Mioblastos/efectos de los fármacos , Polímeros/síntesis química , Polímeros/química , Polímeros/farmacología
4.
Mater Sci Eng C Mater Biol Appl ; 123: 111957, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33812585

RESUMEN

Bioactive glasses have been widely investigated for their ability to release ions with therapeutic effect. In this paper, a silica based bioactive glass was doped with a low amount of tellurium dioxide (1 and 5 mol%) to confer antibacterial and antioxidant properties. The obtained glasses were characterized in terms of morphology, composition, structure, characteristic temperatures and in vitro bioactivity. Moreover, comprehensive analyses were carried out to estimate the cytocompatibility, the antibacterial and antioxidant properties of Te-doped glasses. The performed characterizations demonstrated that the Te insertion did not interfere with the amorphous nature of the glass, the substitution of SiO2 with TeO2 led to a slight decrease in Tg and a TeO2 amount higher than 1 mol% can induce a change in the primary crystal field. In vitro bioactivity test demonstrated the Te-doped glass ability to induce the precipitation of hydroxyapatite. Finally, the biological characterization showed a strong antibacterial and antioxidant effects of Te-containing glasses in comparison with the control glass, demonstrating that Te is a promising element to enhance the biological response of biomaterials.


Asunto(s)
Dióxido de Silicio , Telurio , Materiales Biocompatibles/farmacología , Durapatita , Vidrio
5.
Mater Sci Eng C Mater Biol Appl ; 123: 111959, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33812587

RESUMEN

Bioactive materials play a significant role in biomedical engineering for plethora of applications. To date, there is no evident report on the role of sodium precursors in structural changes towards their acceleration in biocompatibility. This study highlights the impact and role of two different sodium precursors (sodium nitrate and sodium hydroxide) on the structural changes and their potential formulations in biomineralization and biocompatibility. Structural characteristics enunciate the significant crystallization of NaCaPO4, Na2Ca2Si3O9, and Na1.8Ca1.1Si6O14 phases with pertinent Q2 stretching's while using sodium nitrate than sodium hydroxide. XPS spectra authenticate the elevated sodium content while using sodium nitrate as sodium precursor. One-dimensional structures with well faceted morphology and superior alkaline environment preferentially support the biomineralization and bactericidal properties in sodium nitrate-bioglass, was confirmed through structural, morphological, elemental, and antibacterial investigations. Whereas, higher blood and cell-line compatibility with elevated protein adsorption rate is perceived for the bioglass prepared using sodium hydroxide source, and subsequently, higher hemostatic properties are considerably observed with sodium nitrate-bioglass. Higher mechanical stability (ultrasonic measurements) and controlled degradation rate are the stratagems of sodium nitrate to boost the basic criteria of bioactive materials. Hence, it is proposed that sodium nitrate is a highly preferable source to develop bioactive and stable bioglass formulations.


Asunto(s)
Materiales Biocompatibles , Hemostáticos , Materiales Biocompatibles/farmacología , Cerámica/farmacología , Cristalización , Vidrio , Ensayo de Materiales , Sodio , Ingeniería de Tejidos
6.
Mater Sci Eng C Mater Biol Appl ; 123: 112006, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33812626

RESUMEN

Biomaterials based on bioactive glass with gold nanoparticle composites have many applications in tissue engineering due to their tissue regeneration and angiogenesis capacities. The objectives of the study were to develop new composites using bioactive glass with gold nanospheres (BGAuSP) and gold nanocages (BGAuIND), individually introduced in alginate-pullulan (Alg-Pll) polymer, to evaluate their biocompatibility potential, and to compare the obtained results with those achieved when ß-tricalcium phosphate-hydroxyapatite (ßTCP/HA) replaced the BG. The novel composites underwent structural and morphological characterization followed by in vitro viability testing on fibroblast and osteoblast cell lines. Additionally, the biomaterials were subcutaneously implanted in Sprague Dawley rats, for in vivo biocompatibility assessment during 3 separate time frames (14, 30 and 60 days). The biological effects were evaluated by histopathology and immunohistochemistry. The physical characterization revealed the cross-linking between polymers and glasses/ceramics and demonstrated a suitable thermal stability for sterilization processes. The in vitro assays demonstrated adequate form, pore size of composites ranging from few micrometers up to 100 µm, while the self-assembled apatite layer formed after simulated body fluid immersion confirmed the composites' bioactivity. Viability assays have highlighted optimal cellular proliferation and in vitro biocompatibility for all tested composites. Furthermore, based on the in vivo subcutaneous analyses the polymer composites with BGAuNP have shown excellent biocompatibility at 14, 30 and 60 days, exhibiting marked angiogenesis while, tissue proliferation was confirmed by high number of Vimentin positive cells, in comparison with the polymer composite that contains ßTCP/HA, which induced an inflammatory response represented by a foreign body reaction. The obtained results suggest promising, innovative, and biocompatible composites with bioactive properties for future soft tissue and bone engineering endeavours.


Asunto(s)
Nanopartículas del Metal , Ingeniería de Tejidos , Animales , Materiales Biocompatibles/farmacología , Biopolímeros , Cerámica , Vidrio , Oro , Ensayo de Materiales , Nanopartículas del Metal/toxicidad , Ratas , Ratas Sprague-Dawley
7.
Int J Mol Sci ; 22(9)2021 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-33923149

RESUMEN

In addition to their chemical composition various physical properties of synthetic bone substitute materials have been shown to influence their regenerative potential and to influence the expression of cytokines produced by monocytes, the key cell-type responsible for tissue reaction to biomaterials in vivo. In the present study both the regenerative potential and the inflammatory response to five bone substitute materials all based on ß-tricalcium phosphate (ß-TCP), but which differed in their physical characteristics (i.e., granule size, granule shape and porosity) were analyzed for their effects on monocyte cytokine expression. To determine the effects of the physical characteristics of the different materials, the proliferation of primary human osteoblasts growing on the materials was analyzed. To determine the immunogenic effects of the different materials on human peripheral blood monocytes, cells cultured on the materials were evaluated for the expression of 14 pro- and anti-inflammatory cytokines, i.e., IL-6, IL-10, IL-1ß, VEGF, RANTES, IL-12p40, I-CAM, IL-4, V-CAM, TNF-α, GM-CSF, MIP-1α, Il-8 and MCP-1 using a Bio-Plex® Multiplex System. The granular shape of bone substitutes showed a significant influence on the osteoblast proliferation. Moreover, smaller pore sizes, round granular shape and larger granule size increased the expression of GM-CSF, RANTES, IL-10 and IL-12 by monocytes, while polygonal shape and the larger pore sizes increased the expression of V-CAM. The physical characteristics of a bone biomaterial can influence the proliferation rate of osteoblasts and has an influence on the cytokine gene expression of monocytes in vitro. These results indicate that the physical structure of a biomaterial has a significant effect of how cells interact with the material. Thus, specific characteristics of a material may strongly affect the regenerative potential in vivo.


Asunto(s)
Materiales Biocompatibles/farmacología , Sustitutos de Huesos/farmacología , Citocinas/metabolismo , Macrófagos/metabolismo , Osteoblastos/metabolismo , Proliferación Celular , Células Cultivadas , Humanos , Macrófagos/citología , Macrófagos/efectos de los fármacos , Osteoblastos/citología , Osteoblastos/efectos de los fármacos
8.
Int J Mol Sci ; 22(9)2021 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-33923239

RESUMEN

Hydroxyapatite has been used in medicine for many years as a biomaterial or a cover for other biomaterials in orthopedics and dentistry. This study characterized the physicochemical properties (structure, particle size and morphology, surface properties) of Li+- and Li+/Eu3+-doped nanohydroxyapatite obtained using the wet chemistry method. The potential regenerative properties against neurite damage in cultures of neuron-like cells (SH-SY5Y and PC12 after differentiation) were also studied. The effect of nanohydroxyapatite (nHAp) on the induction of repair processes in cell cultures was assessed in tests of metabolic activity, the level of free oxygen radicals and nitric oxide, and the average length of neurites. The study showed that nanohydroxyapatite influences the increase in mitochondrial activity, which is correlated with the increase in the length of neurites. It has been shown that the doping of nanohydroxyapatite with Eu3+ ions enhances the antioxidant properties of the tested nanohydroxyapatite. These basic studies indicate its potential application in the treatment of neurite damage. These studies should be continued in primary neuronal cultures and then with in vivo models.


Asunto(s)
Materiales Biocompatibles/farmacología , Durapatita/farmacología , Nanopartículas/administración & dosificación , Regeneración Nerviosa , Neuroblastoma/tratamiento farmacológico , Nervios Periféricos/citología , Animales , Humanos , Técnicas In Vitro , Nanopartículas/química , Neuroblastoma/patología , Células PC12 , Nervios Periféricos/efectos de los fármacos , Nervios Periféricos/patología , Ratas , Propiedades de Superficie , Células Tumorales Cultivadas
9.
Int J Mol Sci ; 22(4)2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33672027

RESUMEN

Five agarose types (D1LE, D2LE, LM, MS8 and D5) were evaluated in tissue engineering and compared for the first time using an array of analysis methods. Acellular and cellular constructs were generated from 0.3-3%, and their biomechanical properties, in vivo biocompatibility (as determined by LIVE/DEAD, WST-1 and DNA release, with n = 6 per sample) and in vivo biocompatibility (by hematological and biochemical analyses and histology, with n = 4 animals per agarose type) were analyzed. Results revealed that the biomechanical properties of each hydrogel were related to the agarose concentration (p < 0.001). Regarding the agarose type, the highest (p < 0.001) Young modulus, stress at fracture and break load were D1LE, D2LE and D5, whereas the strain at fracture was higher in D5 and MS8 at 3% (p < 0.05). All agaroses showed high biocompatibility on human skin cells, especially in indirect contact, with a correlation with agarose concentration (p = 0.0074 for LIVE/DEAD and p = 0.0014 for WST-1) and type, although cell function tended to decrease in direct contact with highly concentrated agaroses. All agaroses were safe in vivo, with no systemic effects as determined by hematological and biochemical analysis and histology of major organs. Locally, implants were partially encapsulated and a pro-regenerative response with abundant M2-type macrophages was found. In summary, we may state that all these agarose types can be safely used in tissue engineering and that the biomechanical properties and biocompatibility were strongly associated to the agarose concentration in the hydrogel and partially associated to the agarose type. These results open the door to the generation of specific agarose-based hydrogels for definite clinical applications such as the human skin, cornea or oral mucosa.


Asunto(s)
Materiales Biocompatibles/química , Hidrogeles/química , Algas Marinas/química , Sefarosa/química , Ingeniería de Tejidos/métodos , Animales , Materiales Biocompatibles/farmacología , Fenómenos Biomecánicos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Módulo de Elasticidad , Fibroblastos/metabolismo , Estudios de Seguimiento , Voluntarios Sanos , Humanos , Hidrogeles/farmacología , Ratas , Ratas Wistar , Sefarosa/farmacología , Piel/citología , Andamios del Tejido/química
10.
Carbohydr Polym ; 260: 117765, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712123

RESUMEN

Chitosan (CS) combined with hydroxyapatite (HA) was injected into a composite braid, and a hierarchical pore structure scaffold was obtained by freeze drying and cold atmospheric plasma (CAP) technology. The CS/HA/braid scaffold with hierarchical pore structure was analyzed and characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, true color confocal microscopy, improved liquid replacement method, and phosphate buffer solution immersion. The mechanical properties and degradation ability of the scaffold were evaluated through compression test and degradation test. Results showed that HA addition endowed the core of the scaffold with macroscopic pore sizes of 80-180 µm, and CAP treatment endowed the shell of the scaffold with microscopic pore sizes ≤10 µm. All scaffolds exhibited high porosity and swelling rates of ≥80 % and ≥300 %, respectively. The scaffold with a hierarchical pore structure had good mechanical properties and twice the degradation rate. In addition, the treated scaffold precipitated intact spherical HA crystals. Under the synergistic effect of HA and CAP treatment, scaffolds achieved 277.6 % cell viability compared with pure CS scaffold. Overall, this method was feasible for preparing bone scaffolds with hierarchical pore structure for potential bone tissue engineering.


Asunto(s)
Quitosano/química , Durapatita/química , Ingeniería de Tejidos , Andamios del Tejido/química , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Fuerza Compresiva , Liofilización , Ratones , Porosidad
11.
Carbohydr Polym ; 260: 117767, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712125

RESUMEN

Wound healing is a dynamic and intricate process, and newly dressings are urgently needed to promote wound healing over the multiple stages. Herein, two water-soluble adenine-modified chitosan (CS-A) derivatives were synthesized in aqueous solutions and freeze-dried to obtain porous sponge-like dressings. The novel derivatives displayed antibacterial activities against S. aureus and E. coli. Moreover, CS-A derivatives demonstrated excellent hemocompatibility and cytocompatibility, as well as promoted the proliferation of the wound cells by shortening the G1 phase and improving DNA duplication efficiency. The ability of CS-A sponges to promote wound healing was studied in a full-thickness skin defect model. The histological analysis and immunohistochemical staining showed that the wounds treated with CS-A sponges displayed fewer inflammatory cells, and faster regeneration of epithelial tissue, collagen deposition and neovascularization. Therefore, CS-A derivatives have potential application in wound dressings and provide new ideas for the design of multifunctional biomaterials.


Asunto(s)
Adenina/química , Materiales Biocompatibles/química , Quitosano/química , Animales , Vendajes , Materiales Biocompatibles/farmacología , Puntos de Control del Ciclo Celular/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Liofilización , Masculino , Ratones , Porosidad , Ratas , Ratas Sprague-Dawley , Piel/efectos de los fármacos , Piel/patología , Cicatrización de Heridas/efectos de los fármacos
12.
Carbohydr Polym ; 260: 117769, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712127

RESUMEN

Periodontal defect poses a significant challenge in orthopedics. Guided Bone Regeneration (GBR) membrane is considered as one of the most successful methods applied to reconstruct alveolar bone and then to achieve periodontal defect repair/regeneration. In this paper, a novel polyamide-6/chitosan@nano-hydroxyapatite/polyamide-6 (PA6/CS@n-HA/PA6) bilayered tissue guided membranes by combining a solvent casting and an electrospinning technique was designed. The developed PA6/CS@n-HA/PA6 composites were characterized by a series of tests. The results show that n-HA/PA6 and electrospun PA6/CS layers are tightly bound by molecular interaction and chemical bonding, which enhances the bonding strength between two distinct layers. The porosity and adsorption average pore diameter of the PA6/CS@n-HA/PA6 membranes are 36.90 % and 22.61 nm, respectively. The tensile strength and elastic modulus of PA6/CS@n-HA/PA6 composites are 1.41 ± 0.18 MPa and 7.15 ± 1.09 MPa, respectively. In vitro cell culture studies demonstrate that PA6/CS@n-HA/PA6 bilayered scaffolds have biological safety, good bioactivity, biocompatibility and osteoconductivity.


Asunto(s)
Regeneración Ósea , Caprolactama/análogos & derivados , Quitosano/química , Durapatita/química , Membranas Artificiales , Nanoestructuras/química , Polímeros/química , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Regeneración Ósea/efectos de los fármacos , Caprolactama/química , Adhesión Celular/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Ratones , Nanofibras/química , Nanoestructuras/toxicidad , Porosidad , Propiedades de Superficie , Resistencia a la Tracción
13.
Carbohydr Polym ; 260: 117777, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712133

RESUMEN

The combination of alginate, hyaluronic acid and multivalent ions have been reported to form alginate-hyaluronic acid ionic-crosslinking hydrogels for biomedical applications. However, injectable alginate-hyaluronic acid ionic-crosslinking hydrogels with satisfactory shear-thinning property have rarely been reported. In this study, we successfully developed an ionic-crosslinked alginate-hyaluronic acid hydrogel by simple assembly of alginate-hyaluronic acid mixture and Fe3+ complex. This hydrogel could fully recover within seconds after damaged, while displayed shear thinning behavior and good injectability which were contributed by the reversible and dynamic metal-ligand interactions formed via ferric ions and carboxyl groups of the polymers. Moreover, the local degradation of this hydrogel giving the hydrogel sustained ferric ions release property, of which led to potential long-term antibacterial activities against multiple types of bacteria including gram-negative Escherichia coli and gram-positive Staphylococcus aureus, as well as representative oral pathogenic bacteria Streptococcus mutans and Porphyromonas gingivalis.


Asunto(s)
Alginatos/química , Antiinfecciosos/química , Compuestos Férricos/química , Ácido Hialurónico/química , Hidrogeles/química , Animales , Antiinfecciosos/farmacología , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Femenino , Compuestos Férricos/metabolismo , Humanos , Hidrogeles/farmacología , Ratones , Ratones Endogámicos BALB C , Porphyromonas/efectos de los fármacos , Reología , Piel/efectos de los fármacos , Piel/patología , Staphylococcus aureus/efectos de los fármacos , Streptococcus mutans/efectos de los fármacos
14.
Carbohydr Polym ; 260: 117780, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712136

RESUMEN

In this study, we prepared a biomimetic hyaluronic acid oligosaccharides (oHAs)-based composite scaffold to develop a bone tissue-engineered scaffold for stimulating osteogenesis and endothelialization. The functional oHAs products were firstly synthesized, namely collagen/hyaluronic acid oligosaccharides/hydroxyapatite (Col/oHAs/HAP), chitosan/hyaluronic acid oligosaccharides (CTS/oHAs), and then uniformly distributed in poly (lactic-co-glycolic acid) (PLGA) solution followed by freeze-drying to obtain three-dimensional interconnected scaffolds as temporary templates for bone regeneration. The morphology, physicochemical properties, compressive strength, and degradation behavior of the fabricated scaffolds, as well as in vitro cell responses seeded on these scaffolds and in vivo biocompatibility, were investigated to evaluate the potential for bone tissue engineering. The results indicated that the oHAs-based scaffolds can promote the attachment of endothelial cells, facilitate the osteogenic differentiation of MC3T3-E1 and BMSCs, and have ideal biocompatibility and tissue regenerative capacity, suggesting their potential to serve as alternative candidates for bone tissue engineering applications.


Asunto(s)
Materiales Biocompatibles/química , Quitosano/química , Colágeno/química , Ingeniería de Tejidos , Animales , Materiales Biocompatibles/farmacología , Diferenciación Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Durapatita/química , Ácido Hialurónico/química , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Ratones , Oligosacáridos/química , Osteoblastos/citología , Osteoblastos/metabolismo , Osteogénesis/efectos de los fármacos , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/química , Andamios del Tejido/química
15.
Nat Commun ; 12(1): 1773, 2021 03 19.
Artículo en Inglés | MEDLINE | ID: mdl-33741995

RESUMEN

The exploration of artificial luminogens with bright emission has been fully developed with the advancement of synthetic chemistry. However, many of them face problems like weakened emission in the aggregated state as well as poor renewability and sustainability. Therefore, the development of renewable and sustainable luminogens with anti-quenching function in the solid state, as well as to unveil the key factors that influence their luminescence behavior become highly significant. Herein, a new class of natural rosin-derived luminogens with aggregation-induced emission property (AIEgens) have been facilely obtained with good biocompatibility and targeted organelle imaging capability as well as photochromic behavior in the solid state. Mechanistic study indicates that the introduction of the alicyclic moiety helps suppress the excited-state molecular motion to enhance the solid-state emission. The current work fundamentally elucidates the role of alicyclic moiety in luminogen design and practically demonstrates a new source to large-scalely obtain biocompatible AIEgens.


Asunto(s)
Materiales Biocompatibles/química , Colorantes Fluorescentes/química , Luminiscencia , Resinas de Plantas/química , Animales , Materiales Biocompatibles/farmacología , Células COS , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Escherichia coli/efectos de los fármacos , Colorantes Fluorescentes/farmacología , Microscopía Confocal , Estructura Molecular , Movimiento (Física) , Imagen Óptica/métodos , Orgánulos/química , Orgánulos/metabolismo , Resinas de Plantas/farmacología , Relación Estructura-Actividad
16.
Carbohydr Polym ; 259: 117501, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33673978

RESUMEN

The study's purpose was to fabricate a 3-D porous scaffold, in which chitosan was coated onto the pore wall of polycaprolactone (PCL) scaffolds as a bioactive agent to maximize the cell recognition signals, to improve the osteoconductivity of the scaffolds. The pppporogen leaching technique has been modified and used in the fabrication process, comprising of the coating of chitosan over the porogen followed by transferring of coating to the pore wall of the PCL scaffold. The cytotoxicity and hemolysis results indicated chitosan's presence over the surface of the scaffold's pore walls has significantly enhanced its biocompatibility. Scaffolds coated with 2.5 %(w/v) chitosan shows 6.74 % increase in porosity and 207.96 % upsurge in mechanical strength, compared to PCL scaffolds. The Gene-expression also proves the study groups of scaffolds show the minimal osteogenic expression. Therefore, chitosan coating over the scaffold's pore wall's surface opens an unconventional approach for tissue engineering applications.


Asunto(s)
Quitosano/química , Poliésteres/química , Ingeniería de Tejidos , Andamios del Tejido/química , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Adhesión Celular/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Fuerza Compresiva , Eritrocitos/citología , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Hemólisis , Humanos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Microesferas , Osteogénesis/efectos de los fármacos , Parafina/química , Porosidad , Ratas
17.
Carbohydr Polym ; 259: 117640, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33673981

RESUMEN

In this study, the electrospun poly(ε-caprolactone) (PCL)/Chitosan (CS)/curcumin (CUR) nanofiber was fabricated successfully with curcumin loaded chitosan nano-encapsulated particles (CURCSNPs). The morphology of the produced CURCSNPs, PCL, PCL/CS, PCL/CS/CUR, and PCL/CS/CUR electrosprayed with CURCSNPs were analyzed by scanning electron microscopy (SEM). The physicochemical properties and biological characteristics of fabricated nanofibers such as antibacterial, antioxidant, cell viability, and in vivo wound healing efficiency and histological assay were tested. The electrospraying of CURCSNPs on surface PCL/CS/CUR nanofiber resulted in the enhanced antibacterial, antioxidant, cell proliferation efficiencies and higher swelling and water vapor transition rates. In vivo examination and Histological analysis showed PCL/CS/CUR electrosprayed with CURCSNPs led to significant improvement of complete well-organized wound healing process in MRSA infected wounds. These results suggest that the application of PCL/CS/CUR electrosprayed with CURCSNPs as a wound dressing significantly facilitates wound healing with notable antibacterial, antioxidant, and cell proliferation properties.


Asunto(s)
Quitosano/química , Curcumina/química , Nanofibras/química , Nanopartículas/química , Poliésteres/química , Cicatrización de Heridas , Animales , Antibacterianos/química , Antibacterianos/farmacología , Antioxidantes/química , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Línea Celular , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Humanos , Masculino , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Ratones , Nanopartículas/toxicidad , Resistencia a la Tracción , Cicatrización de Heridas/efectos de los fármacos
18.
Carbohydr Polym ; 259: 117707, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33673987

RESUMEN

Chitin hydrogels have multiple advantages of nontoxicity, biocompatibility, biodegradability, and three-dimensional hydrophilic polymer network structure similar to the macromolecular biological tissue. However, the mechanical strength of chitin hydrogels is relatively weak. Construction of chitin hydrogels with high mechanical strength and good biocompatibility is essential for the successful applications in biomedical field. Herein, we developed double crosslinked chitin hydrogels by dissolving chitin in KOH/urea aqueous solution with freezing-thawing process, then using KH560 as cross-linking agent and coagulating in ethanol solution at low temperature. The obtained chitin/ KH560 (CK) hydrogels displayed good transparency and toughness with compressed nanofibrous network and porous structure woven with chitin nanofibers. Moreover, the optimal CK hydrogels exhibited excellent mechanical properties (σb = 1.92 ± 0.21 Mpa; εb = 71 ± 5 %), high swelling ratio, excellent blood compatibility, biocompatibility and biodegradability, which fulfill the requirements of biomedical materials and showing potential applications in biomedicine.


Asunto(s)
Materiales Biocompatibles/química , Quitina/química , Reactivos de Enlaces Cruzados/química , Hidrogeles/química , Animales , Materiales Biocompatibles/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Fuerza Compresiva , Eritrocitos/citología , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Hemólisis/efectos de los fármacos , Ratones , Nanofibras/química , Porosidad , Conejos
19.
Carbohydr Polym ; 259: 117710, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33673989

RESUMEN

Harmful algal blooms induce severe environmental problems. It is challenging to remove algae by the current available treatments involving complicate process and costly instruments. Here, we developed a CaO2@PEG-loaded water-soluble self-branched chitosan (CP-SBC) system, which can remove algae from water in one-step without additional instrumentation. This approach utilizes a novel flocculant (self-branched chitosan) integrated with flotation function (induced by CaO2@PEG). CP-SBC exhibited better flocculation performance than commercial flocculants, which is attributed to the enhanced bridging and sweeping effect of branched chitosan. CP-SBC demonstrated outstanding biocompatibility, which was verified by zebrafish test and algae activity test. CaO2@PEG-loaded self-branched chitosan can serve as an "Air flotation system" to spontaneous float the flocs after flocculation by sustainably released O2. Furthermore, CP-SBC can improve water quality through minimizing dissolved oxygen depletion and reducing total phosphorus concentrations.


Asunto(s)
Quitosano/química , Floraciones de Algas Nocivas/fisiología , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Compuestos de Calcio/química , Floculación/efectos de los fármacos , Floraciones de Algas Nocivas/efectos de los fármacos , Cinética , Larva/efectos de los fármacos , Óxidos/química , Oxígeno/química , Fósforo/química , Polietilenglicoles/química , Porosidad , Pez Cebra/crecimiento & desarrollo , Pez Cebra/fisiología
20.
Molecules ; 26(5)2021 Feb 24.
Artículo en Inglés | MEDLINE | ID: mdl-33668154

RESUMEN

More than 200,000 people are suffering from Anterior Cruciate Ligament (ACL) related injuries each year in the US. There is an unmet clinical demand for improving biological attachment between grafts and the host tissue in addition to providing mechanical support. For biological graft integration, it is important to provide a physiologically feasible environment for the host cells to enable them to perform their duties. However, behavior of cells during ACL healing and the mechanism of ACL healing is not fully understood partly due to the absence of appropriate environment to test cell behavior both in vitro and in vivo. This study aims at (i) investigating the change in fibril diameter of bovine ACL tissue upon injury and (ii) fabricating nanofiber-based scaffolds to represent the morphology and structure of healthy and injured ACL tissues. We hypothesized that distribution and mean diameter of ACL fibrils will be altered upon injury. Findings revealed that the collagen fibril diameter distribution of bovine ACL changed from bimodal to unimodal upon injury with subsequent decrease in mean diameter. Polycaprolactone (PCL) scaffold fiber diameter distribution exhibited similar bimodal and unimodal distribution behavior to qualitatively represent the cases of healthy and injured ACL, respectively. The native ACL tissue demonstrated comparable modulus values only with the aligned bimodal PCL scaffolds. There was significant difference between mechanical properties of aligned bimodal and unaligned unimodal PCL scaffolds. We believe that the results obtained from measurements of diameter of collagen fibrils of native bovine ACL tissue can serve as a benchmark for scaffold design.


Asunto(s)
Ligamento Cruzado Anterior/efectos de los fármacos , Materiales Biocompatibles/farmacología , Nanoestructuras/química , Poliésteres/química , Andamios del Tejido/química , Animales , Ligamento Cruzado Anterior/patología , Materiales Biocompatibles/química , Bovinos , Colágeno/química , Tamaño de la Partícula , Propiedades de Superficie
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