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1.
Georgian Med News ; (311): 27-32, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33814385

RESUMEN

Using the mesh for hernia repair is the most common type of hernia surgery. There are many types of meshes made of various synthetic materials, but all of these meshes have their own respective disadvantages. The aim of this study was to provide preliminary results of a non-randomized clinical trial evaluation of novel porcine grafts XI-S+® (Colorado Therapeutics LLC. USA) for ventral and inguinal hernia repair. All patients underwent a standardized surgical procedure. Onlay surgical repair technique has been performed in ten patients with ventral hernia and Lichtenstein tension-free method has been used for ten patients with inguinal hernia repair. The XI-S+® mesh fixation was performed with multiple simple interrupted sutures using prolene thread. The average age of the patients with ventral hernia was 54±14 years, and 30% of patients were female and 70% of patients were male. The average age of the patients with inguinal hernia was 62.5±9.4 years, and 10% of patients were female and 90% of patients were male. The average hospitalization length was 2 days. During three years of observation, no recurrence of hernia was observed in patients. The XI-S + ® mesh has anti-adhesive properties, is extremely resistant to infections, provides favorable conditions for engraftment, early activity and patient rehabilitation. The clinical studies of the patients that underwent ventral and inguinal hernia repair using XI-S+® mesh have shown that the post-operative pain was minimal and easily controlled by the use of analgesics. As for the sensation of the mesh, in some patients it has been present up until 1 month from surgery, but it fully disappeared by the end of the 3rd month.


Asunto(s)
Hernia Inguinal , Adulto , Anciano , Animales , Materiales Biocompatibles/uso terapéutico , Femenino , Hernia Inguinal/cirugía , Herniorrafia , Humanos , Masculino , Persona de Mediana Edad , Polipropilenos , Recurrencia , Mallas Quirúrgicas , Porcinos , Resultado del Tratamiento
2.
Medicine (Baltimore) ; 100(17): e25703, 2021 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-33907152

RESUMEN

RATIONALE: Three-dimensional (3D) printing has attracted wide attention for its potential and abilities in the assistance of surgical planning and the development of personalized prostheses. We herewith report a unique case of chronic clavicle osteomyelitis treated with a two-stage subtotal clavicle reconstruction using a 3D printed polyether-ether-ketone (PEEK) prosthesis. PATIENT CONCERNS: A 23-year-old Chinese female presented to our clinic complaining about a progressive pain of her right clavicle for about 1 year. DIAGNOSES: Chronic clavicle osteomyelitis confirmed by percutaneous biopsy and lesion biopsy. INTERVENTIONS: This patient accepted a long-term conservative treatment, which did not gain satisfactory outcomes. Thus, a subtotal removal and two-stage reconstruction of the right clavicle with a 3D-printed polyether-ether-ketone prosthesis stabilized by screw fixation system was performed. OUTCOMES: At 2-year follow-up, complete pain relief and satisfactory functional recovery of her right shoulder were observed. LESSONS: Personalized 3D printed prosthesis is an effective and feasible method for reconstruction of complex bone defects.


Asunto(s)
Clavícula , Osteomielitis , Cuidados Preoperatorios/métodos , Impresión Tridimensional , Implantación de Prótesis , Dispositivos de Fijación Quirúrgicos , Tomografía Computarizada por Rayos X/métodos , Materiales Biocompatibles/uso terapéutico , Biopsia/métodos , Enfermedad Crónica , Clavícula/diagnóstico por imagen , Clavícula/patología , Clavícula/cirugía , Femenino , Humanos , Cetonas/uso terapéutico , Osteomielitis/diagnóstico , Osteomielitis/fisiopatología , Osteomielitis/cirugía , Polietilenglicoles/uso terapéutico , Diseño de Prótesis , Implantación de Prótesis/instrumentación , Implantación de Prótesis/métodos , Procedimientos Quirúrgicos Reconstructivos/instrumentación , Procedimientos Quirúrgicos Reconstructivos/métodos , Resultado del Tratamiento , Adulto Joven
3.
Molecules ; 26(3)2021 Jan 29.
Artículo en Inglés | MEDLINE | ID: mdl-33572728

RESUMEN

Volumetric Muscle Loss (VML) is associated with muscle loss function and often untreated and considered part of the natural sequelae of trauma. Various types of biomaterials with different physical and properties have been developed to treat VML. However, much work remains yet to be done before the scaffolds can pass from the bench to the bedside. The present review aims to provide a comprehensive summary of the latest developments in the construction and application of natural polymers-based tissue scaffolding for volumetric muscle injury. Here, the tissue engineering approaches for treating volumetric muscle loss injury are highlighted and recent advances in cell-based therapies using various sources of stem cells are elaborated in detail. An overview of different strategies of tissue scaffolding and their efficacy on skeletal muscle cells regeneration and migration are presented. Furthermore, the present paper discusses a wide range of natural polymers with a special focus on proteins and polysaccharides that are major components of the extracellular matrices. The natural polymers are biologically active and excellently promote cell adhesion and growth. These bio-characteristics justify natural polymers as one of the most attractive options for developing scaffolds for muscle cell regeneration.


Asunto(s)
Músculo Esquelético/efectos de los fármacos , Enfermedades Musculares/tratamiento farmacológico , Regeneración/efectos de los fármacos , Andamios del Tejido/química , Materiales Biocompatibles/uso terapéutico , Matriz Extracelular/efectos de los fármacos , Humanos , Músculo Esquelético/lesiones , Músculo Esquelético/patología , Enfermedades Musculares/patología , Polímeros/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos
4.
PLoS One ; 16(1): e0244635, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33400697

RESUMEN

Polycystic kidney disease (PKD) is a common genetic disorder that results in a proliferating and enlarging cyst and ultimately leads to loss of kidney function. Because an enlarged cyst is a primary factor for limited kidney function, the large cyst is surgically removed by laparoscopic deroofing or sclerosant. This a relatively nascent treatment method entails complications and sometimes fail due to the cyst fluid refilling and infection. This study proposes using a more stable and effective polidocanol foam with glycerol and Rose Bengal (GRP form) to prevent cyst regeneration and irritation, which is caused by the required body movement during the treatment. Specifically, the foam retention time and viscosity were increased by adding glycerol up to 10% (w/v). The GRP form inhibited cellular proliferation and disrupted cellular junctions, e-cadherin, and cyst formation, demonstrated by the LDH, Live and Dead, and re-plating culture assays. The GRP foam was shown to be a safe and effective treatment as a commercial grade polidocanol foam form by an in vivo study in which subcutaneously injected mice injected with commercial 3% polidocanol, and the GRP foam showed no difference in inflammation. Thus, this study provides an advanced polidocanol form by adding glycerol and Rose-Bengal to help existing sclerotherapy.


Asunto(s)
Glicerol/uso terapéutico , Polidocanol/uso terapéutico , Enfermedades Renales Poliquísticas/terapia , Rosa Bengala/uso terapéutico , Soluciones Esclerosantes/uso terapéutico , Animales , Materiales Biocompatibles/uso terapéutico , Perros , Células de Riñón Canino Madin Darby , Masculino , Ratones Endogámicos BALB C , Enfermedades Renales Poliquísticas/patología
5.
Methods Mol Biol ; 2193: 31-40, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-32808256

RESUMEN

In tissue engineering, scaffolds should provide the topological and physical cues as native tissues to guide cell adhesion, growth, migration, and differentiation. Fibrous structure is commonly present in human musculoskeletal tissues, including muscles, tendons, ligaments, and cartilage. Biomimetic fibrous scaffolds are thus critical for musculoskeletal tissue engineering. Electrospinning is a versatile technique for fabricating nanofibers from a variety of biomaterials. However, conventional electrospinning can only generate 2D nanofiber mats. Postprocessing methods are often needed to create 3D electrospun nanofiber scaffolds. In this chapter, we present two novel electrospinning-based scaffold fabrication techniques, which can generate 3D nanofiber scaffolds in one-station process: divergence electrospinning and hybrid 3D printing with parallel electrospinning. These techniques can be applied for engineering tissues with aligned fiber structures.


Asunto(s)
Materiales Biocompatibles/uso terapéutico , Músculo Esquelético/crecimiento & desarrollo , Tendones/crecimiento & desarrollo , Ingeniería de Tejidos/métodos , Diferenciación Celular/efectos de los fármacos , Humanos , Músculo Esquelético/patología , Nanofibras/química , Nanofibras/uso terapéutico , Impresión Tridimensional , Tendones/patología , Andamios del Tejido/química
6.
Int J Biol Macromol ; 169: 321-329, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-33310094

RESUMEN

Osteosarcoma has a high prevalence among children and adolescents. Common treatments of this disease are not promising enough. Molecular processes involved in the pathogenesis of osteosarcoma are not fully understood. Besides, the remnants of tumor cells after surgery can cause bone destruction and recurrence of the disease. Thus, there is a need to develop novel drugs or enhancing the currently-used drugs as well as identifying bone-repairing methods. Chitosan is a natural compound produced by the deacetylation of chitin. Research has shown that chitosan can be used in various fields due to its beneficial effects, such as biodegradability and biocompatibility. Regarding cancer, chitosan exerts several anti-tumor activities. Moreover, it can be used in diagnostic techniques, drug delivery systems, and cell culture methods. Herein, we aim to discuss the potential roles of chitosan in studying and treating osteosarcoma. We review the literature on chitosan's applications as a drug delivery system and how it can be combined with other substances to improve its ability of local drug delivery. We take a look into the studies concerning the possible benefits of chitosan in the field of bone tissue engineering and 3D culturing. Furthermore, anti-cancer activities of different compounds of chitosan are reviewed.


Asunto(s)
Quitosano/uso terapéutico , Osteosarcoma/tratamiento farmacológico , Materiales Biocompatibles/uso terapéutico , Neoplasias Óseas/tratamiento farmacológico , Quitina/química , Quitina/farmacología , Quitosano/metabolismo , Sistemas de Liberación de Medicamentos/métodos , Humanos , Ingeniería de Tejidos/métodos
7.
Nat Nanotechnol ; 16(2): 214-223, 2021 02.
Artículo en Inglés | MEDLINE | ID: mdl-33318641

RESUMEN

Biomaterials can improve the safety and presentation of therapeutic agents for effective immunotherapy, and a high level of control over surface functionalization is essential for immune cell modulation. Here, we developed biocompatible immune cell-engaging particles (ICEp) that use synthetic short DNA as scaffolds for efficient and tunable protein loading. To improve the safety of chimeric antigen receptor (CAR) T cell therapies, micrometre-sized ICEp were injected intratumorally to present a priming signal for systemically administered AND-gate CAR-T cells. Locally retained ICEp presenting a high density of priming antigens activated CAR T cells, driving local tumour clearance while sparing uninjected tumours in immunodeficient mice. The ratiometric control of costimulatory ligands (anti-CD3 and anti-CD28 antibodies) and the surface presentation of a cytokine (IL-2) on ICEp were shown to substantially impact human primary T cell activation phenotypes. This modular and versatile biomaterial functionalization platform can provide new opportunities for immunotherapies.


Asunto(s)
Materiales Biocompatibles/química , ADN/química , Linfocitos T/inmunología , Animales , Presentación de Antígeno , Materiales Biocompatibles/uso terapéutico , Línea Celular Tumoral , Humanos , Inmunoterapia Adoptiva , Activación de Linfocitos , Ratones , Nanopartículas/química , Neoplasias/terapia , Proteínas/química , Proteínas/inmunología , Proteínas/uso terapéutico , Receptores Quiméricos de Antígenos/inmunología , Linfocitos T/trasplante
8.
Dent Clin North Am ; 65(1): 167-195, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33213708

RESUMEN

For successful outcomes in bone grafting, it is important to have a clear and detailed understanding of the fundamentals and basics in regenerative science. This article summarize the grafting materials and growth factors that are now in use to provide an improved understanding of the properties of each material and indications for subsequent use. The article gives an overview of the fundamentals of bone healing, including the physiology of regeneration. It is hoped that clinicians can make improved decisions that are based in literature when considering treatment options for restoring patients' functional dentition.


Asunto(s)
Regeneración Ósea , Trasplante Óseo , Materiales Biocompatibles/uso terapéutico , Humanos , Ingeniería de Tejidos
9.
ACS Biomater Sci Eng ; 6(2): 759-778, 2020 02 10.
Artículo en Inglés | MEDLINE | ID: mdl-33313391

RESUMEN

Despite the success of vaccines in preventing many infectious diseases, effective vaccines against pathogens with ongoing challenges - such as HIV, malaria, and tuberculosis - remain unavailable. The emergence of new pathogen variants, the continued prevalence of existing pathogens, and the resurgence of yet other infectious agents motivate the need for new, interdisciplinary approaches to direct immune responses. Many current and candidate vaccines, for example, are poorly immunogenic, provide only transient protection, or create risks of regaining pathogenicity in certain immune-compromised conditions. Recent advances in biomaterials research are creating new potential to overcome these challenges through improved formulation, delivery, and control of immune signaling. At the same time, many of these materials systems - such as polymers, lipids, and self-assembly technologies - may achieve this goal while maintaining favorable safety profiles. This review highlights ways in which biomaterials can advance existing vaccines to safer, more efficacious technologies, and support new vaccines for pathogens that do not yet have vaccines. Biomaterials that have not yet been applied to vaccines for infectious disease are also discussed, and their potential in this area is highlighted.


Asunto(s)
Materiales Biocompatibles , Enfermedades Transmisibles , Tuberculosis , Vacunas , Materiales Biocompatibles/uso terapéutico , Humanos , Inmunidad , Tuberculosis/prevención & control
10.
ACS Appl Mater Interfaces ; 12(50): 55659-55674, 2020 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-33327053

RESUMEN

Diabetic skin ulcer is one of the severe complications of diabetes mellitus, which has a high incidence and may cause death or disability. Platelet-rich plasma (PRP) is widely used in the treatment of diabetic wounds due to the effect of growth factors (GFs) derived from it. However, the relatively short half-life of GFs limits their applications in clinics. In addition, the presence of a large amount of proteases in the diabetic wound microenvironment results in the degradation of GFs, which further impedes angiogenesis and diabetic wound healing. In our study, we fabricated a self-healing and injectable hydrogel with a composite of chitosan, silk fibroin, and PRP (CBPGCTS-SF@PRP) for promoting diabetic wound healing. CBPGCTS-SF@PRP could protect PRP from enzymatic hydrolysis, release PRP sustainably, and enhance the chemotaxis of mesenchymal stem cells. The results showed that it could promote the proliferation of repair cells in vitro. Moreover, it could enhance wound healing by expediting collagen deposition, angiogenesis, and nerve repair in a type 2 diabetic rat model and a rat skin defect model. We hope that this study will offer a new treatment for diabetic nonhealing wounds in clinics.


Asunto(s)
Materiales Biocompatibles/farmacología , Hidrogeles/química , Plasma Rico en Plaquetas/metabolismo , Cicatrización de Heridas/efectos de los fármacos , Animales , Benzaldehídos/química , Materiales Biocompatibles/química , Materiales Biocompatibles/uso terapéutico , Proliferación Celular/efectos de los fármacos , Quitosano/química , Diabetes Mellitus Tipo 2/inducido químicamente , Diabetes Mellitus Tipo 2/patología , Fibroínas/química , Humanos , Hidrogeles/metabolismo , Péptidos y Proteínas de Señalización Intercelular/farmacología , Masculino , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Ratones , Ratones Desnudos , Neovascularización Fisiológica/efectos de los fármacos , Fibras Nerviosas/fisiología , Plasma Rico en Plaquetas/química , Polietilenglicoles/química , Ratas , Regeneración/efectos de los fármacos , Enfermedades de la Piel/tratamiento farmacológico , Enfermedades de la Piel/patología
11.
Isr Med Assoc J ; 22(12): 736-740, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-33381943

RESUMEN

BACKGROUND: Chronic lung diseases, especially emphysema and pulmonary fibrosis, are the third leading cause of mortality worldwide. Their treatment includes symptom alleviation, slowing of the disease progression, and ultimately organ transplant. Regenerative medicine represents an attractive alternative. OBJECTIVES: To develop a dual approach to lung therapy by engineering a platform dedicated to both remodeling pulmonary architecture (bronchoscopic lung volume reduction) and regeneration of lost respiratory tissue (scaffold). METHODS: The authors developed a hydrogel scaffold based on the natural polymers gelatin and alginate. The unique physical properties allow its injection through long catheters that pass through the working channel of a bronchoscope. The scaffold might reach the diseased area; thus, serving a dual purpose: remodeling the lung architecture as a lung volume reduction material and developing a platform for tissue regeneration to allow for cell or organoid implant. RESULTS: The authors' novel hydrogel scaffold can be injected through long catheters, exhibiting the physical and mechanical properties necessary for the dual treatment objectives. Its biocompatibility was analyzed on human fibroblasts and mouse mesenchymal cells. Cells injected with the scaffold through long narrow catheters exhibited at least 70% viability up to 7 days. CONCLUSIONS: The catheter-injectable gelatin-alginate hydrogel represents a new concept, which combines tissue engineering with minimal invasive procedure. It is an inexpensive and convenient to use alternative to other types of suggested scaffolds for lung tissue engineering. This novel concept may be used for additional clinical applications in regenerative medicine.


Asunto(s)
Hidrogeles/uso terapéutico , Enfermedades Pulmonares/terapia , Ingeniería de Tejidos/métodos , Andamios del Tejido , Alginatos , Animales , Materiales Biocompatibles/administración & dosificación , Materiales Biocompatibles/uso terapéutico , Catéteres , Fibroblastos , Gelatina , Humanos , Hidrogeles/administración & dosificación , Inyecciones , Pulmón , Ratones
12.
Nat Commun ; 11(1): 5696, 2020 11 10.
Artículo en Inglés | MEDLINE | ID: mdl-33173046

RESUMEN

Poorly immunogenic tumors, including triple negative breast cancers (TNBCs), remain resistant to current immunotherapies, due in part to the difficulty of reprogramming the highly immunosuppressive tumor microenvironment (TME). Here we show that peritumorally injected, macroporous alginate gels loaded with granulocyte-macrophage colony-stimulating factor (GM-CSF) for concentrating dendritic cells (DCs), CpG oligonucleotides, and a doxorubicin-iRGD conjugate enhance the immunogenic death of tumor cells, increase systemic tumor-specific CD8 + T cells, repolarize tumor-associated macrophages towards an inflammatory M1-like phenotype, and significantly improve antitumor efficacy against poorly immunogenic TNBCs. This system also prevents tumor recurrence after surgical resection and results in 100% metastasis-free survival upon re-challenge. This chemo-immunotherapy that concentrates DCs to present endogenous tumor antigens generated in situ may broadly serve as a facile platform to modulate the suppressive TME, and enable in situ personalized cancer vaccination.


Asunto(s)
Materiales Biocompatibles/uso terapéutico , Vacunas contra el Cáncer/uso terapéutico , Inmunoterapia/métodos , Neoplasias de la Mama Triple Negativas/terapia , Animales , Antígenos de Neoplasias/metabolismo , Biotecnología/métodos , Linfocitos T CD8-positivos/inmunología , Células Dendríticas/inmunología , Sistemas de Liberación de Medicamentos/métodos , Femenino , Factor Estimulante de Colonias de Granulocitos y Macrófagos/inmunología , Factor Estimulante de Colonias de Granulocitos y Macrófagos/uso terapéutico , Humanos , Factores Inmunológicos/metabolismo , Factores Inmunológicos/uso terapéutico , Macrófagos/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Recurrencia Local de Neoplasia/inmunología , Recurrencia Local de Neoplasia/prevención & control , Neoplasias/inmunología , Neoplasias/terapia , Neoplasias de la Mama Triple Negativas/inmunología , Microambiente Tumoral/inmunología
13.
Clin Orthop Surg ; 12(2): 151-157, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-32489535

RESUMEN

Backgroud: Untreated osteonecrosis of the femoral head ultimately leads to secondary coxarthrosis. The aim of this study was to determinate if the core decompression of the femoral head combined with implantation of autologous bone marrow concentrate with tricalcium phosphate could be used to prevent radiographic progression of early stage osteonecrosis of the hip. We also sought to determine whether this treatment improved clinical outcomes and reduced the need for total hip arthroplasty. Methods: Eighteen hips were included in the present study. All of them underwent core decompression of the femoral head combined with implantation of autologous bone marrow concentrate with tricalcium phosphate between 2007 and 2012. The cell concentrate was obtained from the posterior iliac crest and processed and implanted during the same surgical procedure. Patient demographic data, clinical data including modified Harris hip score, and radiological data were collected preoperatively, postoperatively, and during the follow-up period. Also, survival endpoints were analyzed: time of femoral head collapse and need for total hip arthroplasty. Results: The mean age of patients was 37.8 years (standard deviation [SD], 9.31 years). The mean follow-up was 68.9 months (SD, 15.0 months). In most cases (70.6%), the etiology of the osteonecrosis of the femoral head was corticosteroid use; in the remaining cases, secondary to alcohol use. Core decompression of the femoral head combined with implantation of autologous bone marrow concentrate with tricalcium phosphate did not prevent progression to collapse (< 80% at 5 years) although modified Harris hip scores improved. Overall median survival with the total hip arthroplasty as endpoint was 23 months (95% confidence interval [CI], 14.9 to 31.1 months). Overall median survival time with any degree of collapse as endpoint was 7 months (95% CI, 2.0 to 12.0 months). Conclusions: The results obtained in this study suggest that core decompression combined with implantation of autologous bone marrow concentrate and tricalcium phosphate will not prevent radiographic progression of early stage osteonecrosis of the hip. These finding also suggest that the absence of indications for hip replacement alone is not an indicator of good response to the treatment, and it is important to note the radiological results.


Asunto(s)
Trasplante de Médula Ósea , Fosfatos de Calcio/uso terapéutico , Descompresión Quirúrgica , Necrosis de la Cabeza Femoral/terapia , Adulto , Materiales Biocompatibles/uso terapéutico , Terapia Combinada , Evaluación de la Discapacidad , Progresión de la Enfermedad , Femenino , Necrosis de la Cabeza Femoral/diagnóstico por imagen , Humanos , Ilion/trasplante , Masculino , Persona de Mediana Edad , Trasplante Autólogo
14.
Clin Oral Investig ; 24(7): 2229-2245, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32519234

RESUMEN

OBJECTIVE: A systematic review and meta-analysis was thus conducted to answer the following focused question based on PICO strategy: Is there any 3D matrix biomaterial used for root coverage of human Miller class I and II defects equivalent with the connective tissue graft in localized defects of at least 2 mm and 3 mm? MATERIAL AND METHODS: The search on electronic database included MEDLINE, Cochrane Central Register of Controlled Trials, Clinical Trials.gov, Web of Science, and New Zealand/Australian Clinical Trials. Only randomized clinical trials (RCTs) that compared connective tissue graft (CTG) with at least one 3D matrix alone for root coverage in Class I and II Miller localized defects of at least 2 mm, with at least 6 months follow-up, were included in this systematic review. RESULTS: A total of 14 studies were included for meta-analysis (12 compared CTG with acellular dermal matrix allograft and 2 compared CTG with Xenogenic Collagen Matrix). Relative root coverage showed no significant difference among the materials, for either 2 or 3 mm minimal recessions. For keratinized tissue width, on 2 mm recessions, CTG showed superiority above other biomaterials, but on 3 mm recessions, it seemed to have the same results. The percentage of recessions with complete root coverage for both 2 and 3 mm recessions showed similar results for all biomaterials. CONCLUSIONS: With their limits, the present data concluded that CTG, acellular dermal matrix allograft, and xenogenic collagen matrix provided similar results for root coverage. CLINICAL RELEVANCE: To know if there is a 3D matrix with equivalent predictable results for root coverage, that we could avoid the morbidity of the connective tissue graft for these cases.


Asunto(s)
Materiales Biocompatibles , Tejido Conectivo , Recesión Gingival , Trasplante de Células Madre Hematopoyéticas , Materiales Biocompatibles/uso terapéutico , Encía , Recesión Gingival/cirugía , Humanos , Nueva Zelanda , Colgajos Quirúrgicos , Raíz del Diente , Resultado del Tratamiento
15.
Endodoncia (Madr.) ; 38(1): 28-43, jun. 2020. ilus, tab, graf
Artículo en Español | IBECS | ID: ibc-199207

RESUMEN

OBJETIVO: Los materiales que se emplean para procedimientos dentales en los que el material contacta directamente con el tejido pulpar y tejidos periodontales han de cumplir una serie de propiedades, entre ellas la biocompatibilidad. A su vez, han de ser materiales que eviten el paso de fluidos y microorganismos con el fin de preservar las condiciones óptimas de los tejidos. El objetivo de esta revisión sistemática fue evaluar la porosidad de los diferentes cementos de agregado trióxido mineral (MTA). MÉTODO: Dos investigadores realizaron búsquedas avanzadas en: PubMed Central, PubMed, Web of Science, Scopus, Cochrane Li-brary, Science Direc y Science Database (Proquest) siguiendo los criterios PRISMA. Los criterios de inclusión contemplaban los es-tudios in vitro, publicados en inglés desde enero de 1993 hasta abril de 2019, que analizaran y midieran la porosidad del MTA. Se excluyeron los estudios in vivo, piloto, editoriales, cartas, revisiones sistemáticas, reseñas literarias, resúmenes de conferencias y trabajos de fin de grado. RESULTADOS: Un total de 853 artículos resultó de la búsqueda inicial, de los que solo 19 pasaron los criterios de inclusión, exclusión y el análisis del riesgo de sesgo. En ellos, se analizan distintas técnicas de condensación del MTA, radiopacificadores, sustratos, etc., en busca de materiales que presenten menor porosidad. CONCLUSIONES: La elevada heterogeneidad de los estudios sobre porosidad y el hecho de que éstos no estén estandarizados, pue-de condicionar la validez externa o generalización de los resultados entre los distintos investigadores para un mismo material y procedimiento. En los estudios que realizan una comparación entre varios materiales, concluyen que tanto MTA ProRoot® y Bio-dentineTM obtuvieron mejores resultados en cuanto a porosidad. Número de solicitud (PROSPERO): 124340


OBJECTIVE: Materials used for dental procedures in which the material directly contacts the pulp and periodontal tissues must have a series of characteristics, such as biocompatibility. Equally, they must be materials that prevent the passage of fluids and microorganisms aiming at keeping the optimal conditions of the tissues intact. The objective of this systematic review was to evaluate the porosity of the different cements of mineral trioxide aggregate (MTA). METHOD: Two researchers conducted advanced searches in: PubMed Central, PubMed, Web of Science, Scopus, Cochrane Library, ScienceDirect and ScienceDatabase (Proquest) following the PRISMA criteria. Inclusion criteria took into account in vitro studies, published in English from January 1993 to April 2019, to analyse and measure the porosity of the MTA. In vivo and pilot studies were excluded, as well as editorials, letters, systematic reviews, literary reviews, conference abstracts and dissertations. RESULTS: A total of 853 articles resulted from the initial search, of which only 19 met the criteria for inclusion, exclusion and analysis of the risk of bias. In them, different condensation techniques of the MTA, radiopacifiers, substrates, etc., are analysed in quest of materials with less porosity. CONCLUSIONS: The high heterogeneity of the studies on porosity and the fact that they are not standardised, can condition the external validity or generalisation of the results among the different researchers for the same material and procedure. In the studies that make a comparison between several materials, they conclude that both MTA ProRoot® and BiodentineTM obtained better outcomes in terms of porosity as compared with other marketed brands. Application number (PROSPERO): 124340


Asunto(s)
Humanos , Cementos Dentales/química , Materiales de Obturación del Conducto Radicular/química , Compuestos de Calcio/química , Compuestos de Aluminio/química , Materiales de Obturación del Conducto Radicular/uso terapéutico , Cementos Dentales/uso terapéutico , Compuestos de Calcio/uso terapéutico , Compuestos de Aluminio/uso terapéutico , Materiales Biocompatibles/química , Materiales Biocompatibles/uso terapéutico , Porosidad
16.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 737-743, May-June, 2020. ilus, tab
Artículo en Portugués | LILACS, VETINDEX | ID: biblio-1128945

RESUMEN

Atropelamentos de animais silvestres são frequentes e muitas vezes causam fraturas ósseas que, se não tratadas adequadamente, levam à morte do animal por complicações ou por inabilidade de sobrevivência no seu habitat natural. O objetivo do presente trabalho foi relatar o uso de placa associada ao pino intramedular e ao biovidro 60S (BV60S) para o tratamento de fratura de fêmur em tamanduá-bandeira. O animal foi resgatado pela polícia ambiental com suspeita de atropelamento. Foi sedado para avaliação clínica e radiográfica, que revelou fratura em fêmur direito. Utilizou-se dexmedetomidina como medicação pré-anestésica, midazolam e cetamina para indução, e isoflurano para manutenção. Também foi realizado bloqueio peridural com bupivacaína e morfina. A osteossíntese foi feita com placa bloqueada 2,7 e pino intramedular 2,5. Colocaram-se 4g de BV60S no foco de fratura para favorecer a osteogênese. O paciente teve recuperação funcional imediata do membro acometido. A reparação óssea ocorreu por segunda intenção, observando-se ossificação completa do calo com consolidação clínica, aos 30 dias, e remodelação quase completa, aos 180 dias. Conclui-se que o uso de placa e pino associado ao BV60S é eficiente no tratamento de fratura de fêmur em tamanduá, permitindo a rápida recuperação e a reintrodução do animal na natureza.(AU)


Roadblocks of wild animals are frequent and often cause bone fractures that if not properly treated lead to the death of the animal due to complications or inability to survive in its natural habitat. The objective of the present study was to report the use of plate rod and bioglass 60S (BG60S) for the treatment of femoral fracture in anteater. The animal was rescued by environmental police on suspicion of being hit. It was sedated for clinical and radiographic evaluation, which revealed a fracture in the right femur. Dexmedetomidine was used as preanesthetic medication, midazolam and ketamine for induction, and isoflurane for maintenance. Epidural blockade with bupivacaine and morphine was also performed. Osteosynthesis was done with a locking plate 2.7 and 2.5 intramedullary pin. 4G of BG60S was placed in the focus of fracture to favor osteogenesis. The patient had immediate functional recovery of the affected limb. The bone repair occurred by second intention, with complete ossification of the callus with clinical consolidation at 30 days, and near complete remodeling at 180 days. It is concluded that the use of plate rod to the BG60S is efficient in the treatment of femur fracture in anteater, allowing the rapid recovery and reintroduction of the animal in the wild.(AU)


Asunto(s)
Animales , Cingulados/cirugía , Fracturas del Fémur/veterinaria , Fijación Interna de Fracturas/métodos , Fijación Interna de Fracturas/veterinaria , Osteogénesis/fisiología , Materiales Biocompatibles/uso terapéutico , Fracturas Óseas , Anestesia de Conducción/veterinaria
17.
BMC Oral Health ; 20(1): 148, 2020 05 19.
Artículo en Inglés | MEDLINE | ID: mdl-32429904

RESUMEN

BACKGROUND: Periodontitis is a chronic inflammation of the tooth supporting structures that finally can lead to tooth loss. As chronic periodontitis is associated with systemic diseases multiple approaches have been followed to support regeneration of the destructed tissue. But very few materials are actually used in the clinic. A new and promising group of biomaterials with advantageous biomechanical properties that have the ability to support periodontal regeneration are self-assembling peptides (SAP). However, there is still a lack of 3D periodontal models that can evaluate the migration potential of such novel materials. METHODS: All experiments were performed with primary human periodontal ligament fibroblasts (HPLF). Migration capacity was assessed in a three-dimensional model of the human periodontal ligament by measuring the migration distance of viable cells on coated (Enamel Matrix Protein (EMP), P11-4, collagen I) or uncoated human dentin. Cellular metabolic activity on P11-4 hydrogels was assessed by a metabolic activity assay. Deposition of ECM molecules in a P11-4 hydrogel was visualized by immunostaining of collagen I and III and fibrillin I. RESULTS: The 3D periodontal model was feasible to show the positive effect of EMP for periodontal regeneration. Subsequently, self-assembling peptide P11-4 was used to evaluate its capacity to support regenerative processes in the 3D periodontal model. HPLF coverage of the dentin surface coated with P11-4 increased significantly over time, even though delayed compared to EMP. Cell viability increased and inclusion of ECM proteins into the biomaterial was shown. CONCLUSION: The presented results indicate that the 3D periodontal model is feasible to assess periodontal defect coverage and that P11-4 serves as an efficient supporter of regenerative processes in the periodontal ligament. CLINICAL RELEVANCE: The establishment of building-block synthetic polymers offers new opportunities for clinical application in dentistry. Self-assembling peptides represent a new generation of biomaterials as they are able to respond dynamically to the changing environment of the biological surrounding. Especially in the context of peri-implant disease prevention and treatment they enable the implementation of new concepts.


Asunto(s)
Materiales Biocompatibles/uso terapéutico , Ligamento Periodontal , Periodontitis/terapia , Periodoncio/fisiología , Ingeniería de Tejidos/métodos , Materiales Biocompatibles/química , Fibroblastos , Humanos , Regeneración
18.
Artículo en Inglés | MEDLINE | ID: mdl-32459078

RESUMEN

Right ventricle to pulmonary artery conduits such as homografts and pre-manufactured synthetic conduits are widely employed in the present era of complex congenital cardiac surgeries for disorders involving right ventricle - pulmonary artery discontinuity and major coronary artery crossing the right ventricular outflow tract. The key drawback of homograft conduits is the need for reoperation to replace them as a result of degeneration over time or in cases where a child has outgrown the conduit and cost is a major drawback to using commercially available conduits. The advantages of expanded polytetrafluoroethylene are its long-term durability in terms of conduit calcification/degeneration and valve stenosis/regurgitation and its cost-effectiveness.  In this video presentation, we demonstrate the preparation and intraoperative usage of a handmade, trileaflet, valved polytetrafluoroethylene conduit in a case of double outlet right ventricle with valvular and subvalvular pulmonary stenosis and the left anterior descending artery crossing the right ventricular outflow tract. Our experience with this handmade conduit is close to 120 cases and we have seen excellent postoperative recovery and results. Our medium-term follow-up echocardiographic evidence shows conduit patency and preserved valve function.


Asunto(s)
Procedimientos Quirúrgicos Cardíacos , Ventrículo Derecho con Doble Salida/cirugía , Ventrículos Cardíacos/cirugía , Diseño de Prótesis/métodos , Arteria Pulmonar/cirugía , Anastomosis Quirúrgica/instrumentación , Anastomosis Quirúrgica/métodos , Materiales Biocompatibles/uso terapéutico , Procedimientos Quirúrgicos Cardíacos/instrumentación , Procedimientos Quirúrgicos Cardíacos/métodos , Catéteres , Niño , Humanos , Masculino , Politetrafluoroetileno/uso terapéutico , Implantación de Prótesis/métodos , Reoperación/instrumentación , Reoperación/métodos , Resultado del Tratamiento
19.
J Nanobiotechnology ; 18(1): 65, 2020 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-32345308

RESUMEN

Nanoparticle based gene delivery systems holds great promise. Superparamagnetic iron oxide nanoparticles (SPIONs) are being heavily investigated due to good biocompatibility and added diagnostic potential, rendering such nanoparticles theranostic. Yet, commonly used cationic coatings for efficient delivery of such anionic cargos, results in significant toxicity limiting translation of the technology to the clinic. Here, we describe a highly biocompatible, small and non-cationic SPION-based theranostic nanoparticles as novel gene therapy agents. We propose for the first-time, the usage of the microRNA machinery RISC complex component Argonaute 2 (AGO2) protein as a microRNA stabilizing agent and a delivery vehicle. In this study, AGO2 protein-conjugated, anti-HER2 antibody-linked and fluorophore-tagged SPION nanoparticles were developed (SP-AH nanoparticles) and used as a carrier for an autophagy inhibitory microRNA, MIR376B. These functionalized nanoparticles selectively delivered an effective amount of the microRNA into HER2-positive breast cancer cell lines in vitro and in a xenograft nude mice model of breast cancer in vivo, and successfully blocked autophagy. Furthermore, combination of the chemotherapy agent cisplatin with MIR376B-loaded SP-AH nanoparticles increased the efficacy of the anti-cancer treatment both in vitro in cells and in vivo in the nude mice. Therefore, we propose that AGO2 protein conjugated SPIONs are a new class of theranostic nanoparticles and can be efficiently used as innovative, non-cationic, non-toxic gene therapy tools for targeted therapy of cancer.


Asunto(s)
Proteínas Argonauta/química , Autofagia , Materiales Biocompatibles/uso terapéutico , Neoplasias de la Mama/tratamiento farmacológico , Nanopartículas de Magnetita/química , MicroARNs/metabolismo , Animales , Anticuerpos/química , Anticuerpos/inmunología , Antineoplásicos/química , Antineoplásicos/uso terapéutico , Autofagia/efectos de los fármacos , Beclina-1/genética , Beclina-1/metabolismo , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Neoplasias de la Mama/patología , Línea Celular Tumoral , Cisplatino/química , Cisplatino/uso terapéutico , Femenino , Humanos , Ratones , Ratones Desnudos , MicroARNs/química , Receptor ErbB-2/inmunología , Trasplante Heterólogo
20.
Int J Nanomedicine ; 15: 2045-2058, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32273701

RESUMEN

Bone regeneration remains a great clinical challenge. Two-dimensional materials, especially graphene and its derivative graphene oxide, have been widely used for bone regeneration. Since its discovery in 2014, black phosphorus (BP) nanomaterials including BP nanosheets and BP quantum dots have attracted considerable scientific attention and are considered as prospective graphene substitutes. BP nanomaterials exhibit numerous advantages such as excellent optical and mechanical properties, electrical conductivity, excellent biocompatibility, and good biodegradation, all of which make them particularly attractive in biomedicine. In this review, we comprehensively summarize recent advances of BP-based nanomaterials in bone regeneration. The advantages are reviewed, the different synthesis methods of BP are summarized, and the applications to promote bone regeneration are highlighted. Finally, the existing challenges and perspectives of BP in bone regeneration are briefly discussed.


Asunto(s)
Regeneración Ósea/fisiología , Nanoestructuras/química , Nanoestructuras/uso terapéutico , Fósforo/química , Animales , Materiales Biocompatibles/química , Materiales Biocompatibles/uso terapéutico , Regeneración Ósea/efectos de los fármacos , Grafito/química , Humanos , Puntos Cuánticos/química
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