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1.
Microbiome ; 12(1): 68, 2024 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-38570877

RESUMEN

BACKGROUND: The trophic strategy is one key principle to categorize microbial lifestyles, by broadly classifying microorganisms based on the combination of their preferred carbon sources, electron sources, and electron sinks. Recently, a novel trophic strategy, i.e., chemoorganoautotrophy-the utilization of organic carbon as energy source but inorganic carbon as sole carbon source-has been specifically proposed for anaerobic methane oxidizing archaea (ANME-1) and Bathyarchaeota subgroup 8 (Bathy-8). RESULTS: To further explore chemoorganoautotrophy, we employed stable isotope probing (SIP) of nucleic acids (rRNA or DNA) using unlabeled organic carbon and 13C-labeled dissolved inorganic carbon (DIC), i.e., inverse stable isotope labeling, in combination with metagenomics. We found that ANME-1 archaea actively incorporated 13C-DIC into RNA in the presence of methane and lepidocrocite when sulfate was absent, but assimilated organic carbon when cellulose was added to incubations without methane additions. Bathy-8 archaea assimilated 13C-DIC when lignin was amended; however, their DNA was derived from both inorganic and organic carbon sources rather than from inorganic carbon alone. Based on SIP results and supported by metagenomics, carbon transfer between catabolic and anabolic branches of metabolism is possible in these archaeal groups, indicating their anabolic versatility. CONCLUSION: We provide evidence for the incorporation of the mixed organic and inorganic carbon by ANME-1 and Bathy-8 archaea in the environment. Video Abstract.


Asunto(s)
Archaea , Metano , Archaea/genética , Marcaje Isotópico , Oxidación-Reducción , Metano/metabolismo , Carbono/metabolismo , ADN , Anaerobiosis , Sedimentos Geológicos , Filogenia
2.
Microb Cell Fact ; 23(1): 102, 2024 Apr 04.
Artículo en Inglés | MEDLINE | ID: mdl-38575972

RESUMEN

BACKGROUND: Poultry feather waste has a potential for bioenergy production because of its high protein content. This research explored the use of chicken feather hydrolysate for methane and hydrogen production via anaerobic digestion and bioelectrochemical systems, respectively. Solid state fermentation of chicken waste was conducted using a recombinant strain of Bacillus subtilis DB100 (p5.2). RESULTS: In the anaerobic digestion, feather hydrolysate produced maximally 0.67 Nm3 CH4/kg feathers and 0.85 mmol H2/day.L concomitant to COD removal of 86% and 93%, respectively. The bioelectrochemical systems used were microbial fuel and electrolysis cells. In the first using a microbial fuel cell, feather hydrolysate produced electricity with a maximum cell potential of 375 mV and a current of 0.52 mA. In the microbial electrolysis cell, the hydrolysate enhanced the hydrogen production rate to 7.5 mmol/day.L, with a current density of 11.5 A/m2 and a power density of 9.26 W/m2. CONCLUSIONS: The data indicated that the sustainable utilization of keratin hydrolysate to produce electricity and biohydrogen via bioelectrical chemical systems is feasible. Keratin hydrolysate can produce electricity and biofuels through an integrated aerobic-anaerobic fermentation system.


Asunto(s)
Pollos , Plumas , Animales , Anaerobiosis , Pollos/metabolismo , Hidrógeno/metabolismo , Queratinas/metabolismo , Metano/metabolismo , Biocombustibles , Reactores Biológicos
3.
J Environ Manage ; 356: 120588, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38518497

RESUMEN

In the agricultural sector, ruminants are the largest methane (CH4) emission source and many efforts have been undertaken to reduce these greenhouse gas emissions, while compromising animal health and physiology. On the other hand, ruminal CH4, which is biomethane, is in high demand, especially in its liquid form (LBM) that can be used as high energy density fuel. However, CH4 released from a ruminant is immediately mixed with air and highly diluted (<0.1%), challenging CH4 capture technologies. Here we aimed to construct a cryogenic pilot system to capture and liquefy enteric CH4 released from dairy cows kept in respiration chambers. To approach this aim, the outlet air from the chambers was directed through a two-step cooling trap to capture CO2 (-120 to -130 °C) as a solid in the first and CH4 and O2 as liquids in the second cooler (-160 to -180 °C). Warming the second cooler resulted in the evaporation of O2, thereby separating O2 and CH4. LBM purity was in average 90% and was lowest at warming rates higher than 0.88 °C/min. The mean CH4 capture efficiency was 92% and found to be independent of sequestration time and flow rate. However, an increase in CH4 concentration to 0.6%, as it occurs directly at the muzzle of a cow, reduced the sequestration time for CH4. These results show that cryogenic technology can be used to obtain LBM from the air containing ultra-low CH4 concentrations as it is found in cattle barns with high efficiency and purity.


Asunto(s)
Metano , Leche , Femenino , Bovinos , Animales , Leche/química , Proyectos Piloto , Metano/análisis , Rumiantes , Agricultura , Dieta/veterinaria , Lactancia
4.
J Environ Manage ; 356: 120615, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38518499

RESUMEN

Anaerobic digestion (AD) is a prevalent waste activated sludge (WAS) treatment, and optimizing methane production is a core focus of AD. Two DESs were developed in this study and significantly increased methane production, including choline chloride-urea (ChCl-Urea) 390% and chloride-ethylene glycol (ChCl-EG) 540%. Results showed that ChCl-Urea mainly disrupted extracellular polymeric substances (EPS) structures, aiding in initial sludge solubilization during pretreatment. ChCl-EG, instead, induced sludge self-driven organic solubilization and enhanced hydrolysis and acidification processes during AD process. Based on the extent to which the two DESs promoted AD for methane production, the AD process can be divided into stage Ⅰ and stage Ⅱ. In stage Ⅰ, ChCl-EG promoted methanogenesis more significantly, microbiological analysis showed both DESs enriched aceticlastic methanogens-Methanosarcina. Notably, ChCl-Urea particularly influenced polysaccharide-related metabolism, whereas ChCl-EG targeted protein-related metabolism. In stage Ⅱ, ChCl-Urea was more dominant than ChCl-EG, ChCl-Urea bolstered metabolism and ChCl-EG promoted genetic information processing in this stage. In essence, this study investigated the microbial mechanism of DES-enhanced sludge methanogenesis and provided a reference for future research.


Asunto(s)
Disolventes Eutécticos Profundos , Aguas del Alcantarillado , Aguas del Alcantarillado/química , Anaerobiosis , Eliminación de Residuos Líquidos/métodos , Colina/química , Metano , Urea/química , Reactores Biológicos
5.
J Environ Manage ; 356: 120750, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38520849

RESUMEN

The nitrate denitrifying anaerobic methane oxidation-anaerobic ammonia oxidation (DAMO-anammox) can accomplish nitrogen removal and methane (CH4) reduction. This process greatly contributes to carbon emission mitigation and carbon neutrality. In this study, we investigated the electron transfer process of functional microorganisms in the iron-mediated DAMO-anammox system. Fe3+ could be bound to several functional groups (-CH3, COO-, -CH) in extracellular polymeric substance (EPS), and the functional groups bound were different at different iron concentration. Fe3+ underwent reduction reactions to produce Fe2+. Most Fe3+ and Fe2+ react with microorganisms and formed chelates with EPS. Three-dimensional fluorescence spectra showed that Fe3+ affected the secretion of tyrosine and tryptophan, which were essential for cytochrome synthesis. The presence of Fe3+ accelerated c-type cytochrome-mediated extracellular electron transfer (EET), and when more Fe3+ existed, the more cytochrome C expressed. DAMO archaea (M. nitroreducens) in the system exhibited a high positive correlation with the functional genes (resa and ccda) for cytochrome c synthesis. Some denitrifying microorganisms showed positive correlations with the abundance of riboflavin. This finding showed that riboflavin secreted by functional microorganisms acted as an electron shuttle. In addition, DAMO archaea were positively correlated with the hair synthesis gene pily1, which indicated that direct interspecies electron transfer (DIET) may exist in the iron-mediated DAMO-anammox system.


Asunto(s)
Compuestos de Amonio , Hierro , Oxidación Anaeróbica del Amoníaco , Matriz Extracelular de Sustancias Poliméricas/metabolismo , Citocromos c/metabolismo , Electrones , Desnitrificación , Anaerobiosis , Archaea , Oxidación-Reducción , Metano , Carbono/metabolismo , Riboflavina/metabolismo , Reactores Biológicos , Compuestos de Amonio/metabolismo , Nitrógeno/metabolismo , Nitritos/metabolismo
6.
J Environ Manage ; 356: 120676, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38520850

RESUMEN

Zeolite was shown to mitigate anaerobic digestion (AD) inhibition caused by several inhibitors such as long-chain fatty acids, ammonia, and phenolic compounds. In this paper, we verified the genericity of zeolite's mitigating effect against other types of inhibitors found in AD such as salts, antibiotics, and pesticides. The impacts of inhibitors and zeolite were assessed on AD performance and microbial dynamics. While sodium chloride and erythromycin reduced methane production rates by 34% and 32%, zeolite mitigated the inhibition and increased methane production rates by 72% and 75%, respectively, compared to conditions without zeolite in the presence of these two inhibitors. Noticeably, zeolite also enhanced methane production rate by 51% in the uninhibited control condition. Microbial community structure was analyzed at two representative dates corresponding to the hydrolysis/fermentation and methanogenesis stages through 16S rRNA gene sequencing. The microbial characteristics were further evidenced with common components analysis. Results revealed that sodium chloride and erythromycin inhibited AD by targeting distinct microbial populations, with more pronounced inhibitory effects during hydrolysis and VFAs degradation phases, respectively. Zeolite exhibited a generic effect on microbial populations in different degradation stages across all experimental conditions, ultimately contributing to the enhanced AD performance and mitigation of different inhibitions. Typically, hydrolytic and fermentative bacteria such as Cellulosilyticum, Sedimentibacter, and Clostridium sensu stricto 17, VFAs degraders such as Mesotoga, Syntrophomonas, and Syntrophobacter, and methanogens including Methanobacterium, Methanoculleus, and Methanosarcina were strongly favored by the presence of zeolite. These findings highlighted the promising use of zeolite in AD processes for inhibition mitigation in general.


Asunto(s)
Zeolitas , Anaerobiosis , Zeolitas/farmacología , Zeolitas/química , ARN Ribosómico 16S/genética , Cloruro de Sodio , Bacterias/genética , Eritromicina/metabolismo , Metano , Reactores Biológicos/microbiología
7.
Proc Natl Acad Sci U S A ; 121(14): e2315568121, 2024 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-38530900

RESUMEN

Methanogenic archaea inhabiting anaerobic environments play a crucial role in the global biogeochemical material cycle. The most universal electrogenic reaction of their methane-producing energy metabolism is catalyzed by N    5-methyl-tetrahydromethanopterin: coenzyme M methyltransferase (MtrABCDEFGH), which couples the vectorial Na+ transport with a methyl transfer between the one-carbon carriers tetrahydromethanopterin and coenzyme M via a vitamin B12 derivative (cobamide) as prosthetic group. We present the 2.08 Šcryo-EM structure of Mtr(ABCDEFG)3 composed of the central Mtr(ABFG)3 stalk symmetrically flanked by three membrane-spanning MtrCDE globes. Tetraether glycolipids visible in the map fill gaps inside the multisubunit complex. Putative coenzyme M and Na+ were identified inside or in a side-pocket of a cytoplasmic cavity formed within MtrCDE. Its bottom marks the gate of the transmembrane pore occluded in the cryo-EM map. By integrating Alphafold2 information, functionally competent MtrA-MtrH and MtrA-MtrCDE subcomplexes could be modeled and thus the methyl-tetrahydromethanopterin demethylation and coenzyme M methylation half-reactions structurally described. Methyl-transfer-driven Na+ transport is proposed to be based on a strong and weak complex between MtrCDE and MtrA carrying vitamin B12, the latter being placed at the entrance of the cytoplasmic MtrCDE cavity. Hypothetically, strongly attached methyl-cob(III)amide (His-on) carrying MtrA induces an inward-facing conformation, Na+ flux into the membrane protein center and finally coenzyme M methylation while the generated loosely attached (or detached) MtrA carrying cob(I)amide (His-off) induces an outward-facing conformation and an extracellular Na+ outflux. Methyl-cob(III)amide (His-on) is regenerated in the distant active site of the methyl-tetrahydromethanopterin binding MtrH implicating a large-scale shuttling movement of the vitamin B12-carrying domain.


Asunto(s)
Mesna , Metiltransferasas , Mesna/metabolismo , Metiltransferasas/metabolismo , Metilación , Vitamina B 12/metabolismo , Metano/metabolismo , Amidas , Vitaminas
8.
J Environ Manage ; 356: 120552, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38531128

RESUMEN

Partial replacement of mineral fertilisers (MF) with animal manures is a good alternative to reduce MF use and increase both nutrient cycling in agriculture and soil organic matter. However, the adoption of this practice must not lead to increased environmental impacts. In this two-year study conducted in an apple orchard, MF were partially replaced with various animal manures, including cattle slurry (CS), acidified cattle slurry (ACS), solid cattle manure (CsM), or poultry manure (PM), and their impacts on greenhouse gas emission (GHG: CO2, N2O and CH4) were examined. A control (CTRL) receiving only MF served as the baseline, representing the conventional scenario in orchard fertilisation. Overall, replacing MF with manures increased GHG emissions, with the magnitude of the impacts depending on the specific characteristics of the manures and the amount of nutrients and organic matter applied. Comparing to the CTRL, application of ACS and CS led to higher CH4 and N2O emissions, while PM application increased both N2O and CO2 emissions. In contrast, replacement with PM and CsM decreased CH4 emissions. Nevertheless, results varied between the two years, influenced by several factors, including soil conditions. While acidification showed potential to mitigate CH4 emissions, it also led to increased N2O emissions compared to CS, particularly in 2022, suggesting the need for further investigation to avoid emission trade-offs. Replacement with CS (20.49 t CO2-eq ha-1) and CsM (20.30 t CO2-eq ha-1) showed comparable global warming potential (GWP) to the conventional scenario (CTRL, 19.49 t CO2-eq ha-1), highlighting their potential as viable MF substitutes.


Asunto(s)
Malus , Estiércol , Animales , Bovinos , Fertilizantes , Dióxido de Carbono/análisis , Óxido Nitroso/análisis , Suelo , Agricultura , Minerales , Aves de Corral , Metano
9.
J Environ Manage ; 356: 120733, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38531140

RESUMEN

In this work, nano zero-valent iron (nZVI) was added to a lab-scale continuous stirring tank reactor (CSTR) for food waste slurry treatment, and the effect of dosing rate and dosage of nZVI were attempted to be changed. The results showed that anaerobic digestion (AD) efficiency and biomethanation stability were optimum under the daily dosing and dosage of 0.48 g/gTCOD. The average daily methane (CH4) yield reached 495.38 mL/gTCOD, which was 43.65% higher than that at control stage, and the maximum CH4 content reached 95%. However, under single dosing rate conditions, high nZVI concentrations caused microbial cell rupture and loosely bound extracellular polymeric substances (LB-EPS) precipitation degradation. The daily dosing rate promoted the hydrogenotrophic methanogenesis pathway, and the activity of coenzyme F420 increased by 400.29%. The microbial analysis indicated that daily addition of nZVI could promote the growth of acid-producing bacteria (Firmicutes and Bacteroidetes) and methanogens (Methanothrix).


Asunto(s)
Eliminación de Residuos , Aguas del Alcantarillado , Anaerobiosis , Hierro , Metano , Alimentos , Reactores Biológicos
10.
J Environ Manage ; 356: 120679, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38531141

RESUMEN

Introduction of alpine grasses to low altitude regions has long been a crucial strategy for enriching germplasm diversity, cultivating and acclimating high-quality species, enhancing ecosystem resilience and adaptability, as well as facilitating ecosystem restoration. However, there is an urgent need to investigate the impacts of planting Gramineae seeds on greenhouse gas (GHG) emissions, particularly during the critical stage of early plant growth. In this study, four species of grass seeds (Stipa breviflora, Poa pratensis, Achnatherum splendens, Elymus nutans) were collected from 19 high-altitude regions surrounding the Qinghai-Tibet Plateau and sown at low-altitude. Measurements of GHG emissions at early seedling growth in the mesocosm experiment using static chamber method showed a strong increase in the cumulative emissions of CO2 (5.71%-9.19%) and N2O (11.36%-13.64%) (p < 0.05), as well as an elevated CH4 uptake (2.75%-5.50%) in sites where the four grass species were introduced, compared to bare soil. Consequently, there was a substantial rise in global warming potential (13.87%-16.33%) (p < 0.05) at grass-introduced sites. Redundancy analysis showed that seed traits, plant biomass, and seedling emergence percentage were the main driving biotic factors of three GHGs fluxes. Our study unveils the potential risk of escalating GHG emissions induced by introducing high altitude grasses to low altitude bare soil, elucidating the mechanism through linking seed traits with seedling establishment and environmental feedback. Furthermore, this offers a new perspective for assessing the impact of grass introduction on ecological environment of introduced site.


Asunto(s)
Calentamiento Global , Gases de Efecto Invernadero , Ecosistema , Plantones/química , Poaceae , Altitud , Suelo , Metano/análisis , Óxido Nitroso/análisis , Dióxido de Carbono/análisis
11.
J Environ Manage ; 356: 120718, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38537467

RESUMEN

Global climate change is predicted to increase exogenous N input into terrestrial ecosystems, leading to significant changes in soil C-cycling. However, it remains largely unknown how these changes affect soil C-cycling, especially in semi-arid grasslands, which are one of the most vulnerable ecosystems. Here, based on a 3-year field study involving N additions (0, 25, 50, and 100 kg ha-1 yr-1 of urea) in a semi-arid grassland on the Loess Plateau, we investigated the impact of urea fertilization on plant characteristics, soil properties, CO2 and CH4 emissions, and microbial C cycling genes. The compositions of genes involved in C cycling, including C fixation, degradation, methanogenesis, and methane oxidation, were determined using metagenomics analysis. We found that N enrichment increased both above- and belowground biomasses and soil organic C content, but this positive effect was weakened when excessive N was input (N100). N enrichment also altered the C-cycling processes by modifying C-cycle-related genes, specifically stimulating the Calvin cycle C-fixation process, which led to an increase in the relative abundance of cbbS, prkB, and cbbL genes. However, it had no significant effect on the Reductive citrate cycle and 3-hydroxypropionate bi-cycle. N enrichment led to higher soil CO2 and CH4 emissions compared to treatments without added N. This increase showed significant correlations with C degradation genes (bglA, per, and lpo), methanogenesis genes (mch, ftr, and mcr), methane oxidation genes (pmoA, pmoB, and pmoC), and the abundance of microbial taxa harboring these genes. Microbial C-cycling genes were primarily influenced by N-induced changes in soil properties. Specifically, reduced soil pH largely explained the alterations in methane metabolism, while elevated available N levels were mainly responsible for the shift in C fixation and C degradation genes. Our results suggest that soil N enrichment enhances microbial C-cycling processes and soil CO2 and CH4 emissions in semi-arid ecosystems, which contributes to more accurate predictions of ecosystem C-cycling under future climate change.


Asunto(s)
Ecosistema , Pradera , Dióxido de Carbono/análisis , Suelo/química , Metano/análisis , Fertilización
12.
J Environ Manage ; 356: 120578, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38547826

RESUMEN

Domestic organic waste resources have increased over the past decade and treatment of this waste via co-digested biogasification facilities is increasing annually. However, inspection standards for such facilities are not well-established. Herein, we aimed to derive calculation formulas and factors related to organic matter decomposition efficiency and methane production rate in accordance with waste treatment facility inspection standards. We also aimed to determine the optimum waste mixing ratio. Sample (field) surveys of 18 treatment facilities and complete enumeration of 110 facilities were conducted. Calculation formulas and factors were derived using the survey data and biochemical methane potential (BMP) test. The calculated coefficients derived through the BMP test were 0.512 m3 CH4/kgVSin for food waste, 0.601 m3 CH4/kgVSin for livestock manure, and 0.382 m3 CH4/kgVSin for sewage sludge. The final derived calculation factors were 65.0% for food waste, 36.0% for livestock manure, and 20.0% for sewage sludge for organic matter decomposition efficiency, and 0.380 m3 CH4/kgVSin for food waste, 0.27 m3 CH4/kgVSin for livestock manure, and 0.140 m3 CH4/kgVSin for sewage sludge for methane production rates. The derived effective capacity calculation factors can be utilized in future waste treatment facility inspection methods by aiding in the establishment of appropriate inspection standards for co-digested biogasification facilities other than single food waste treatment facilities. In addition, the optimum mixing ratio can be used as design data for co-digested biogasification facilities.


Asunto(s)
Eliminación de Residuos , Aguas del Alcantarillado , Aguas del Alcantarillado/química , Anaerobiosis , Alimentos , Estiércol/análisis , Reactores Biológicos , Metano/análisis , Digestión , República de Corea
13.
J Hazard Mater ; 469: 133921, 2024 May 05.
Artículo en Inglés | MEDLINE | ID: mdl-38452670

RESUMEN

Increasing evidence indicates that metal oxides can improve the methanogenic performance during anaerobic digestion (AD) of piggery wastewater. However, the impacts of composite metal oxides on the methanogenic performance and risk of antibiotic resistance gene (ARG) transmission during AD are not fully understood. In this study, different concentrations of Fe-Mn binary oxides (FMBO at 0, 250, 500, and 1000 mg/L) were added to AD to explore the effects of FMBO on the process. The methane yield was 7825.1 mL under FMBO at 250 mg/L, 35.2% higher than that with FMBO at 0 mg/L. PICRUSt2 functional predictions showed that FMBO promoted the oxidation of acetate and propionate, and the production of methane from the substrate, as well as increasing the abundances of most methanogens and genes encoding related enzymes. Furthermore, under FMBO at 250 mg/L, the relative abundances of 14 ARGs (excluding tetC and sul2) and four mobile gene elements (MGEs) decreased by 24.7% and 55.8%, respectively. Most of the changes in the abundances of ARGs were explained by microorganisms, especially Bacteroidetes (51.20%), followed by MGEs (11.98%). Thus, the methanogenic performance of AD improved and the risk of horizontal ARG transfer decreased with FMBO, especially at 250 mg/L.


Asunto(s)
Antibacterianos , Óxidos , Óxidos/química , Anaerobiosis , Farmacorresistencia Microbiana/genética , Metano , Genes Bacterianos
14.
Water Res ; 254: 121387, 2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38457943

RESUMEN

Constructed wetlands (CWs) are frequently used for effective biological treatment of nitrogen-rich wastewater with external carbon source addition; however, these approaches often neglect the interaction between plant litter and biochar in biochar-amended CW environments. To address this, we conducted a comprehensive study to assess the impacts of single or combined addition of common reed litter and reed biochar (pyrolyzed at 300 and 500 °C) on nitrogen removal, greenhouse gas emission, dissolved organic matter (DOM) dynamics, and microbial activity. The results showed that combined addition of reed litter and biochar to CWs significantly improved nitrate and total nitrogen removal compared with biochar addition alone. Compared to those without reed litter addition, CWs with reed litter addition had more low-molecular-weight and less aromatic DOM and more protein-like fluorescent DOM, which favored the enrichment of bacteria associated with denitrification. The improved nitrogen removal could be attributed to increases in denitrifying microbes and the relative abundance of functional denitrification genes with litter addition. Moreover, the combined addition of reed litter and 300 °C-heated biochar significantly decreased nitrous oxide (30.7 %) and methane (43.9 %) compared to reed litter addition alone, while the combined addition of reed litter and 500 °C-heated biochar did not. This study demonstrated that the presences of reed litter and biochar in CWs could achieve both high microbial nitrogen removal and relatively low greenhouse gas emissions.


Asunto(s)
Carbón Orgánico , Gases de Efecto Invernadero , Humedales , Desnitrificación , Nitrógeno , Materia Orgánica Disuelta , Metano
15.
Water Res ; 254: 121422, 2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38460225

RESUMEN

Methanogenesis of waste biomass (WB) is a promising method for global sustainable development, reduction of pollution and carbon emission levels, and recovering bioenergy. Unlike in the methanogenesis of organic wastewater, in which microbial cells come into direct contact with the dissolved substrate, the 'solid-liquid-solid' modes in WB and between WB and microbial cells, which involve numerous solid-liquid interfaces, greatly hinder the methanogenesis efficiency of WB. Amongst all WB, waste activated sludge is the most complex, poorly biodegradable and representative. Herein, we highlight the role of water evaporation-driven solid-liquid interfacial restructuring of sludge in determining its methanogenesis efficiency. Non-free water evaporation increased surface roughness and adhesion, and compressed pore structure with numerous capillaries in sludge, resulting in a new solid-liquid interface of sludge with great capillary force and highly ordered interfacial water molecules, which provides an extremely favourable condition for high mass transfer and proton-coupled electron transfer (PCET) in sludge. This restructuring was confirmed to induce the enhancement of solid-liquid interfacial noncovalent interactions and electron transfer efficiency in the subsequent methanogenesis process (P < 0.05), promoting the effective contact between the sludge substrate and microbial cells, thereby enriching the methanogenic consortia (i.e., Clostridia and Methanosarcina were increased by 290.0 % and 239.7 %, respectively) and improving the activities of key enzymes. Stable isotope tracing and metagenomic analysis further reveal that this restructuring promoted the participation of water molecules in the methane formation by PCET-driven release of protons from water, and enhanced main methanogenesis metabolic pathways, especially the metabolic pathway of CO2-reduction methanogenesis (+65.2 %), thereby resulting in a great advance in methane generation (+147 %, P < 0.001). The findings can provide a reference for regulating directional anaerobic biotransformation of water-rich multiphase complex substrates by interfacial restructuring inducement.


Asunto(s)
Reactores Biológicos , Aguas del Alcantarillado , Aguas del Alcantarillado/química , Anaerobiosis , Biomasa , Metano , Agua
16.
J Environ Manage ; 356: 120546, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38471321

RESUMEN

Anaerobic granular sludge (AGS) has been regarded as the core of lots of advanced anaerobic reactors. Formation of biogenic Fe products and their incorporation into AGS could influence interspecies electron transfer and methanogenesis performance. In this study, with anaerobic granular sludge (AGS) from different sources (brewery, chemical plant, paper mill, citric acid factory, and food factory) as the research targets, the formation of biogenic iron products in AGS through the biologically induced mineralization process was studied. Furthermore, the influences of physicochemical properties and microbial community on methanogenesis were investigated. Results showed that all the AGS of different sources possessed the capacity to form biogenic Fe products through dissimilatory iron-reduction process, and diverse Fe minerals including magnetite (Fe3O4), hematite (Fe2O3), goethite (FeOOH), siderite (FeCO3) and wustite (FeO) were incorporated into AGS. The AGS loaded with Fe minerals (Fe-AGS) showed increased conductivity, magnetism and zeta-potential comparing to the control. Those Fe-AGS of different sources demonstrated different methanogenesis performance during the long-term operation (50 days). Methane production was increased for the Fe-AGS of citric acid (6.99-32.50%), food (8.33-37.46%), chemical (2.81-7.22%) and brewery plants (2.27-2.81%), but decreased for the Fe-AGS of paper mill (54.81-72.2%). The changes of microbial community and microbial correlations in AGS as a response to Fe minerals incorporation were investigated. For the Fe-AGS samples with enhanced methane production capability, it was widely to find the enriched populations of fermentative and dissimilatory iron reducing bacteria Clostridium_sensu_stricto_6, Bacteroidetes_vadinHA17 and acetoclastic methanogens Methanosaeta, and positive correlations between them. This study provides comprehensive understanding on the effects of incorporation biogenic Fe products on AGS from different sources.


Asunto(s)
Microbiota , Aguas del Alcantarillado , Aguas del Alcantarillado/química , Anaerobiosis , Hierro/química , Óxido Ferrosoférrico , Metano , Ácido Cítrico , Reactores Biológicos
17.
Chemosphere ; 354: 141666, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38494001

RESUMEN

While anaerobic digestion (AD) has been employed for the degradation of chlorinated aliphatic hydrocarbons, the associated digester performance might suffer from volatile fatty acids accumulation, insufficient substrate-microbes interaction, and lower biogas yields. To overcome these limitations, this study is the first to augment the hydrocarbon-degrading microbial capacities by adding agricultural waste-based biochar to the digestion medium. 1,2-dichloroethane (1,2-DCA) was selected as the target pollutant because it is discharged in large quantities from oil refining, petrochemical, and chemical industries, causing serious environmental and human health concerns. A multi-chamber anaerobic reactor (MAR) was operated at a 1,2-DCA loading rate of 1.13 g/L/d, glucose dosage (as an electron donor) range of 200-700 mg/L, and hydraulic retention time of 11.2 h, giving dechlorination = 32.2 ± 6.9% and biogas yield = 210 ± 30 mL/g CODremoved. These values increased after biochar supplementation (100 mg/g volatile solids, VS, as an inoculum carrier) up to 60.2 ± 11.5% and 290 ± 40 mL/g CODremoved, respectively, owing to the enhancement of dehydrogenase enzyme activities. Burkholderiales (15.3%), Clostridiales (2.3%), Bacteroidales (3.5%), Xanthomonadales (3.3%), and Rhodobacterales (6.1%) involved in 1,2-DCA degradation were dominant in the reactor supplemented with biochar. It's suggested that biochar played a major role in facilitating the direct interspecies electron transfer (DIET) between syntrophic bacteria and methanogens, where chloride, ethylene glycol, and acetate derived from 1,2-DCA dechlorination could be further used to promote methanogenesis and methane production. The synergetic effect of adsorption and dechlorination towards 1,2-DCA removal was validated at various biochar dosages (50-120 mg/g) and 1,2-DCA concentrations (50-1000 mg/L). The techno-economic results showed that the cost of treating 1,2-DCA-laden discharge (100 m3/d) by the MAR module could be 0.83 USD/m3 with a payback period of 6.24 years (NPV = 2840 USD and IRR = 10%), retrieving profits from pollution reduction (9542 USD/yr), biogas selling (10418 USD/yr), and carbon credit (10294 USD/yr).


Asunto(s)
Reactores Biológicos , Dicloruros de Etileno , Microbiota , Humanos , Anaerobiosis , Biocombustibles , Carbón Orgánico , Metano
18.
Chemosphere ; 354: 141732, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38499072

RESUMEN

Malignant invasive Erigeron canadensis, as a typical lignocellulosic biomass, is a formidable challenge for sustainable and efficient resource utilization, however nanobubble water (NBW) coupled with anaerobic digestion furnishes a prospective strategy with superior environmental and economic effectiveness. In this study, influence mechanism of various O2-NBW addition times on methanogenic performance of E. canadensis during anaerobic digestion were performed to achieve the optimal pollution-free energy conversion. Results showed that supplementation of O2-NBW in digestion system could significantly enhance the methane production by 10.70-16.17%, while the maximum cumulative methane production reached 343.18 mL g-1 VS in the case of one-time O2-NBW addition on day 0. Furthermore, addition of O2-NBW was conducive to an increase of 2-90% in the activities of dehydrogenase, α-glucosidase and coenzyme F420. Simultaneously, both facultative bacteria and methanogenic archaea were enriched as well, further indicating that O2-NBW might be responsible for facilitating hydrolytic acidification and methanogenesis. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) cluster analysis, provision of O2-NBW enhanced the metabolism of carbohydrate and amino acid, translation as well as membrane transport of bacteria and archaea. This study might offer the theoretical guidance and novel insights for efficient recovery of energy from lignocellulosic biomass on account of O2-NBW adhibition in anaerobic digestion system, progressing tenor of carbon-neutral vision.


Asunto(s)
Erigeron , Anaerobiosis , Agua , Bacterias , Archaea , Suplementos Dietéticos , Metano , Reactores Biológicos , Aguas del Alcantarillado/química
19.
Environ Sci Pollut Res Int ; 31(16): 24461-24479, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38441735

RESUMEN

Animal waste can be converted into a renewable energy source using biogas technology. This process has an impact on greenhouse gas emissions and is a sustainable source of energy for countries. It can reduce the effects of climate change and protect the planet for future generations. Tier1 and tier2 approaches are commonly used in the literature to calculate emissions factors. With boosting algorithms, this study estimated each animal category's biogas potential and CH4 emissions (tier1 and tier2 approach) for 2004-2021 in all of Turkey's provinces. Two different scenarios were created in the study. For scenario-1, the years 2020-2021 were predicted using data from 2004 to 2019, while for scenario-2, the years 2022-2024 were predicted using data from 2004 to 2021. According to the scenario-1 analysis, the eXtreme Gradient Boosting Regressor (XGBR) algorithm was the most successful algorithm with an R2 of 0.9883 for animal-based biogas prediction and 0.9835 and 0.9773 for animal-based CH4 emission predictions (tier1 and tier2 approaches) for the years 2020-2021. When the mean absolute percentage error was evaluated, it was found to be relatively low at 0.46%, 1.07%, and 2.78%, respectively. According to the scenario-2 analysis, the XGBR algorithm predicted the log10 values of the animal-based biogas potential of five major cities in Turkey for the year 2024, with 11.279 for Istanbul, 12.055 for Ankara, 12.309 for Izmir, 11.869 for Bursa, and 12.866 for Antalya. In the estimation of log10 values of CH4 emission, the tier1 approach yielded estimates of 3.080, 3.652, 3.929, 3.411, and 3.321, respectively, while the tier2 approach yielded estimates of 1.810, 2.806, 2.757, 2.552 and 2.122, respectively.


Asunto(s)
Biocombustibles , Aves de Corral , Animales , Bovinos , Estiércol , Turquia , Metano/análisis , Rumiantes
20.
Pol J Microbiol ; 73(1): 107-120, 2024 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-38437466

RESUMEN

Hydrocarbon constituents of petroleum are persistent, bioaccumulated, and bio-magnified in living tissues, transported to longer distances, and exert hazardous effects on human health and the ecosystem. Bioaugmentation with microorganisms like bacteria is an emerging approach that can mitigate the toxins from environmental sources. The present study was initiated to target the petroleum-contaminated soil of gasoline stations situated in Lahore. Petroleum degrading bacteria were isolated by serial dilution method followed by growth analysis, biochemical and molecular characterization, removal efficiency estimation, metabolites extraction, and GC-MS of the metabolites. Molecular analysis identified the bacterium as Bacillus cereus, which exhibited maximum growth at 72 hours and removed 75% petroleum. Biochemical characterization via the Remel RapID™ ONE panel system showed positive results for arginine dehydrolase (ADH), ornithine decarboxylase (ODC), lysine decarboxylase (LDC), o-nitrophenyl-ß-D-galactosidase (ONPG), p-nitrophenyl-ß-D-glucosidase (ßGLU), p-nitrophenyl-N-acetyl-ß-D-glucosaminidase (NAG), malonate (MAL), adonitol fermentation (ADON), and tryptophane utilization (IND). GC-MS-based metabolic profiling identified alcohols (methyl alcohol, o-, p- and m-cresols, catechol, and 3-methyl catechol), aldehydes (methanone, acetaldehyde, and m-tolualdehyde), carboxylic acid (methanoic acid, cis,cis-muconic acid, cyclohexane carboxylic acid and benzoic acid), conjugate bases of carboxylic acids (benzoate, cis,cis-muconate, 4-hydroxybenzoate, and pyruvate) and cycloalkane (cyclohexene). It suggested the presence of methane, methylcyclohexane, toluene, xylene, and benzene degradation pathways in B. cereus.


Asunto(s)
Bacillus cereus , Ecosistema , Humanos , Bacillus cereus/genética , Hidrocarburos , Metano , Ácidos Carboxílicos
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