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1.
Orv Hetil ; 162(15): 579-586, 2021 04 02.
Artículo en Húngaro | MEDLINE | ID: mdl-33798103

RESUMEN

Összefoglaló. Az immunrendszer nem megfelelo muködése meghatározó szerepet játszik a daganatok kialakulásában, progressziójában és az egyes terápiák hatékonyságában is. A bélrendszer baktériumai a szervezet immunitásán keresztül képesek befolyásolni a szervezet gyógyszeres terápiákra adott válaszreakcióját, kiváltképpen az immunellenorzopont-gátló kezelések hatását. Az újgenerációs nukleinsav-szekvenálási technológiák felhasználásával részletes képet kaphatunk a szervezetben jelen lévo baktériumok minoségi és mennyiségi viszonyairól. A közelmúltban összefüggést igazoltak a vastagbéldaganat, a melanoma, a vesesejtes carcinoma és a nem kissejtes tüdorák esetén alkalmazott immunellenorzopont-gátló terápiák hatékonysága és a bél mikrobiom-összetétele között. Számos olyan baktériumot azonosítottak, melynek jelenlétébol, illetve mennyiségébol következtethetünk az egyes kezelésekkel szembeni egyéni érzékenységre. Ezzel összhangban, az antibiotikumkezelés által okozott dysbiosis növelte az immunellenorzopont-gátló terápia sikertelenségének kockázatát. Ezen eredmények tükrében a jövoben a mikrobiom-összetétel meghatározása is fontos tényezo lehet az immunterápiák hatékonyságának elorejelzésében, illetve egyre inkább bizonyított, hogy a széles spektrumú antibiotikumkezelés a legtöbbször csökkenti a daganatellenes immunterápiák hatékonyságát. Jelenleg folyó klinikai vizsgálatok pedig a mikrobiom-összetétel mesterséges úton történo megváltoztatásának terápiás lehetoségeit tanulmányozzák. Bebizonyosodott, hogy a korábbi állásponttal szemben a vizelet nem steril. DNS-szekvenálás alkalmazásával számos olyan, a vizeletben eloforduló baktériumot sikerült azonosítani, melynek jelenléte hozzájárulhat a húgyhólyagrák kialakulásához és progressziójához, illetve a húgyhólyagban lokálisan alkalmazott BCG-terápia hatékonyságához. Jelen munkában a közelmúlt publikációit feldolgozva összefoglaljuk, mely baktériumok jelenléte hozható összefüggésbe a különbözo daganatok kialakulásával, progressziójával és terápiarezisztenciájával. Orv Hetil. 2020; 162(15): 579-586. Summary. Dysfunction of the immune system plays a crucial role in the development and progression of cancer as well as the effectiveness of antitumor therapies. Gut microbiota, due to their impact on the immune system, are able to influence response to anticancer drug therapies. Next-generation DNA-sequencing technologies enabled a comprehensive quantitative and qualitative exploration of the gut microbiome. An increasing body of evidence indicates the association between the efficacy of immune checkpoint inhibitor therapies and gut microbiome composition in colorectal cancer, malignant melanoma, renal cell carcinoma, and non-small cell lung cancer. Recently, several bacterial strains and species were shown to be associated with treatment efficacies. In accordance, dysbiosis caused by antibiotic treatment was found to increase the risk of failure to immune checkpoint inhibitor therapies. In the light of these results, examination of microbiome composition may become an important factor for the prediction of immunotherapies. Currently ongoing clinical trials are investigating the potential of therapeutic alteration of microbiome composition. Contrary to the previous view, urine has been shown not to be sterile. By using sensitive DNA-sequencing technologies, several urinary bacteria could be identified which may contribute to the development and progression of bladder cancer and may influence the efficacy of intravesical BCG therapy. In the present work, we summarize recent studies that identified the presence of certain bacteria associated with the development, progression, and therapy resistance of various cancers. Orv Hetil. 2020; 162(15): 579-586.


Asunto(s)
Microbiota , Neoplasias , Microbioma Gastrointestinal/inmunología , Humanos , Microbiota/inmunología , Neoplasias/inmunología , Neoplasias/terapia , Orina/microbiología
2.
Georgian Med News ; (311): 46-50, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33814389

RESUMEN

Along with sexually transmitted infections opportunistic pathogens and normal microbiota are becoming increasingly clinically important. Opportunistic pathogens can lead to purulent-inflammatory process of the genitals The aim of the current work was to detect qualitative and quantitative composition of vagina contents in non-specific bacterial vaginosis. Vaginal microbiota of 298 women aged from 16 to 64 years old was analyzed. Examination was provided by polymerase chain reaction. In normocenosis with domination of enterobacteria the prevalence index of conditionally-pathogenic microflora wasn't more than 3 lg GE/sample. With absence of mycoplasmas the quantity of conditionally-pathogenic microorganisms wasn't more than 104,5. At same time in patients with dysbiosis of I and II levels index of conditionally-pathogenic microflora was from 3 to 1 and less than 1 lg GE/sample accordingly; there was diagnosed a significant decrease of the total bacterial mass. Simultaneously, decreasing of Lactobacillus quantity (more expressed in dysbiosis-II) meanwhile appearance of mycoplasmas and utmost constant quantity of Candida was revealed. A comprehensive determination of a wide range of opportunistic pathogens on the background of normal flora in the vaginal discharge of women of different ages had been studied.


Asunto(s)
Microbiota , Excreción Vaginal , Vaginosis Bacteriana , Adolescente , Adulto , Anciano , Disbiosis , Femenino , Humanos , Persona de Mediana Edad , Vagina , Adulto Joven
3.
Front Cell Infect Microbiol ; 11: 625581, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33659220

RESUMEN

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the pandemic Coronavirus Disease 2019 (COVID-19). This virus is highly transmissible among individuals through both droplets and aerosol leading to determine severe pneumonia. Among the various factors that can influence both the onset of disease and the severity of its complications, the microbiome composition has also been investigated. Recent evidence showed the possible relationship between gut, lung, nasopharyngeal, or oral microbiome and COVID-19, but very little is known about it. Therefore, we aimed to verify the relationships between nasopharyngeal microbiome and the development of either COVID-19 or the severity of symptoms. To this purpose, we analyzed, by next generation sequencing, the hypervariable V1-V2-V3 regions of the bacterial 16S rRNA in nasopharyngeal swabs from SARS-CoV-2 infected patients (n=18) and control (CO) individuals (n=12) using Microbiota solution A (Arrow Diagnostics). We found a significant lower abundance of Proteobacteria and Fusobacteria in COVID-19 patients in respect to CO (p=0.003 and p<0.0001, respectively) from the phylum up to the genus (p<0.001). The Fusobacterium periodonticum (FP) resulted as the most significantly reduced species in COVID-19 patients respect to CO. FP is reported as being able to perform the surface sialylation. Noteworthy, some sialic acids residues on the cell surface could work as additional S protein of SARS-CoV-2 receptors. Consequently, SARS-CoV-2 could use sialic acids as receptors to bind to the epithelium of the respiratory tract, promoting its clustering and the disease development. We can therefore speculate that the significant reduction of FP in COVID-19 patients could be directly or indirectly linked to the modulation of sialic acid metabolism. Finally, viral or environmental factors capable of interfering with sialic metabolism could determine a fall in the individual protection from SARS-CoV-2. Further studies are necessary to clarify the precise role of FP in COVID-19.


Asunto(s)
/epidemiología , Infecciones por Fusobacterium/microbiología , Fusobacterium/crecimiento & desarrollo , Microbiota , Ácido N-Acetilneuramínico/metabolismo , Pandemias , /aislamiento & purificación , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Fusobacterium/genética , Humanos , Masculino , Persona de Mediana Edad , Boca/microbiología , Nasofaringe/microbiología
4.
Environ Sci Process Impacts ; 23(3): 491-500, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33647083

RESUMEN

The objective of this analysis was to examine and compare quantitative metrics of observed dampness and mold, including visible mold and moisture damage, and fungal and bacterial microbiomes. In-home visits were conducted at age 7 for children enrolled in the Cincinnati Childhood Allergy and Air Pollution Study. Trained study staff evaluated the primary residence and measured total areas of visible moisture and mold damage in the home. Floor dust was collected and archived. Archived dust samples collected from each home (n = 178) were extracted and analyzed using bacterial (16S rRNA gene) and fungal (internal transcribed spacer region) sequencing. Fungi were also divided into moisture requirement categories of xerophiles, mesophiles, and hydrophiles. Data analyses used Spearman's correlation, Kruskal-Wallis, Permanova, DESeq, and negative binomial regression models. Comparing high moisture or mold damage to no damage, five fungal species and two bacterial species had higher concentrations (absolute abundance) and six fungal species and three bacterial species had lower concentrations. Hydrophilic and mesophilic fungi showed significant dose-related increases with increasing moisture damage and mold damage, respectively. When comparing alpha or beta diversity of fungi and bacteria across mold and moisture damage levels, no significant associations or differences were found. Mold and moisture damage did not affect diversity of fungal and bacterial microbiomes. Instead, both kinds of damage were associated with changes in species composition of both bacterial and fungal microbiomes, indicating that fungal and bacterial communities in the home might be influenced by one another as well as by mold or moisture in the home.


Asunto(s)
Contaminación del Aire Interior , Microbiota , Contaminación del Aire Interior/análisis , Bacterias/genética , Niño , Polvo/análisis , Hongos/genética , Vivienda , Humanos , ARN Ribosómico 16S
5.
Bioresour Technol ; 329: 124905, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33676351

RESUMEN

Parameters of temperature-phased anaerobic digestion (TPAD) were varied to study their effects on hydrolysis, biomethane potential (BMP), and microbial diversity of microalgae biodegradation. Anaerobic pretreatments at 85 °C demonstrated the release of soluble carbohydrate and protein molecules under low microbial metabolic activity. However, at 55 °C, anaerobic pretreatments showed superior performance in methane yield, nutrient release, and volatile fatty acids (VFAs) production due to dominant Clostridium. Furthermore, the highest destruction of volatile solids (VS) was observed during aerobic pretreatments at 55 °C under the influence of various quantities of these genera - Luteimonas, Symbiobacterium, Soehngenia, Thermobacillus, and Ureibacillus. Statistical analysis revealed that hydrolysis and BMP were not correlated. However, soluble nitrogen and phosphorous showed strong correlation with methane (r = 0.623 and 0.948, respectively) under thermo-anaerobic pretreatment, while VS removal and concentrations of acetic and butyric acids and lipids were positively correlated with each other under thermo-aerobic pretreatment.


Asunto(s)
Microalgas , Microbiota , Anaerobiosis , Hidrólisis , Metano
6.
Bioresour Technol ; 329: 124919, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33676353

RESUMEN

Links between synergy and microbial community characteristics in co-digestion of food waste (FW), cattle manure (CM) and corn straw (CS) were investigated. Mono-digestion of FW and CS were inhibited by organic acids. Co-digestion of FW with CM achieved greater synergistic rates (18.5% and 22.3%) than CM with CS (14.8% and 12.3%). Synergy resulted from coupling effects of improving nutrient balance, dilution of toxic compounds, higher buffering capacity, detoxification based on co-metabolism, which ultimately reflected in microbial community functions. Although co-digestion of FW with CS exhibited lowest synergistic rates (7.9% and 4.9%), detoxification based on co-metabolism of syntrophic communities of Syntrophomonadaceae with hydrogenotrophic methanogens accelerated system recovery. Digester with the greatest synergy (65% FW + 35% CM) maintained dominant growth of hydrogenotrophic methanogens (68.9%), highest methanogenic community diversity and relative abundance of Methanosarcina (14.6%), which sustained more diverse and switchable methanogenic pathways therefore ensured powerful methanogenic functions and vigorous methanogenic capability.


Asunto(s)
Microbiota , Eliminación de Residuos , Anaerobiosis , Animales , Biocombustibles , Reactores Biológicos , Bovinos , Digestión , Alimentos , Estiércol , Metano , Zea mays
7.
Bioresour Technol ; 329: 124918, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33684839

RESUMEN

Sulfur-driven autotrophic denitrification (SDAD) is feasible for the treatment of low-C/N-ratio and sulfur-laden wastewaters. The nitrite accumulated in SDAD will affect the performance and stability of the system but can be a potential electron acceptor. Thus, single- and multiple-electron acceptor-mediated SDAD systems were investigated. Batch assays revealed that nitrite and nitrate were the preferential options in the SDAD system with single and multiple electron acceptors, respectively. Synchronous nitrogen and sulfur removal was successfully achieved in continuous flow experiments with multiple electron acceptors, and the system could adapt well to high concentrations of sulfide, nitrate and nitrite (i.e., 720, 108 and 64.8 mg L-1, respectively), with the predominant genera shifting from Thiobacillus (48.88%) at the initial stage to unclassified_p_Firmicute (34.24%) and Syner-01 (12.31%) at the last stage. This work provides a fundamental basis for applying and regulating SDAD with multiple electron acceptors for the remediation of nitrogen- and sulfide- laden wastewaters.


Asunto(s)
Microbiota , Nitrógeno , Procesos Autotróficos , Reactores Biológicos , Desnitrificación , Electrones , Nitratos , Azufre
8.
Bioresour Technol ; 329: 124928, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33690060

RESUMEN

In current study, the enhancement effect of magnetite on anaerobic digestion was evaluated at increased organic loading rate (OLR) from 1.6 to 25.6 kg COD·m-3·d-1. The supplement of magnetite enhanced the methane yield by 7-483% accompanied with faster VFAs conversion. Microbial analysis suggested the varied enhancing effect achieved at different OLRs was attributed to different syntrophic interactions triggered by magnetite. More specially, an electroactive syntropy was established between Trichococcus with Methanobacterium at OLR lower than 6.4 kg COD·m-3·d-1, while with the OLR increase, more acid fermentative bacteria (Propionimicrobium, Syner-01) were enriched and further enhanced methanogenesis in a syntrophic way with Methanosaeta. Overall, the incorporation of magnetite was a promising approach to achieve efficient anaerobic digestion, OLR was also critical factor affecting the methanogenesis and should be carefully regulated in future application.


Asunto(s)
Reactores Biológicos , Microbiota , Anaerobiosis , Óxido Ferrosoférrico , Metano
9.
Medicine (Baltimore) ; 100(8): e24311, 2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33663048

RESUMEN

RATIONALE: Necrotizing periodontal diseases (NPDs) are a group of infectious diseases varying in severity, and microorganisms are responsible for these diseases. Currently, the oral microbiota in early disease has been poorly investigated; thus, the causative pathogen and dynamic alteration of the microbiome in NPDs remain unclear. PATIENT CONCERNS: We report a case of a 33-year-old female patient with severe gingival pain and localized necrotizing ulcerative gingival lesions. Conventional therapy was performed, but the necrotizing lesion continued to develop. DIAGNOSES: X-ray examination showed marginal alveolar bone loss in the involved teeth. Histological examination of a biopsy from the gingival lesion showed chronic inflammatory cell infiltration in the tissue, and no cancer cells were observed. Subgingival swabs were taken from the ulcerative gingiva and the gingiva that was not yet affected, and the composition of the microbiota was analyzed by targeted pyrosequencing of the V3-V4 hypervariable regions of the small subunit ribosomal RNA. We found that Neisseria spp., Corynebacterium spp., and Prevotella spp. were clearly enriched in the lesion site. However, Fusobacteria was more abundant in the not-yet-affected gingiva, and Leptotrichia spp. were the most abundant phylotype. INTERVENTIONS: After clinical assessment, a tooth with poor prognosis was extracted, and minocycline hydrochloride was locally administered in the involved tooth pocket every day. Additionally, the patient received 100 mg of hydrochloric acid doxycycline twice per day. OUTCOMES: Remarkable improvement was obtained after 3 days, and the lesion completely healed after 1 week. The follow-up examination 1 year later showed a complete recovery with no recurrent episodes of pain. LESSONS: Changes in the subgingival microbiome can occurr before clinical symptoms appears, and Fusobacteria may be involved in the imbalance of the subgingival flora in the early stage of NPDs. Moreover, Neisseria is a potential bacterial candidate that deserves further study.


Asunto(s)
Periodontitis Crónica/microbiología , Periodontitis Crónica/patología , Microbiota/fisiología , Adulto , Pérdida de Hueso Alveolar , Antibacterianos/uso terapéutico , Periodontitis Crónica/tratamiento farmacológico , Periodontitis Crónica/cirugía , Femenino , Humanos , Necrosis
10.
Int J Food Microbiol ; 344: 109115, 2021 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-33662901

RESUMEN

Development of bloater defect in cucumber fermentations is the result of carbon dioxide (CO2) production by the indigenous microbiota. The amounts of CO2 needed to cause bloater defect in cucumber fermentations brined with low salt and potential microbial contributors of the gas were identified. The carbonation of acidified cucumbers showed that 28.68 ± 6.04 mM (12%) or higher dissolved CO2 induces bloater defect. The microbiome and biochemistry of cucumber fermentations (n = 9) brined with 25 mM calcium chloride (CaCl2) and 345 mM sodium chloride (NaCl) or 1.06 M NaCl were monitored on day 0, 2, 3, 5, 8, 15 and 21 using culture dependent and independent microbiological techniques and High-Performance Liquid Chromatography. Changes in pH, CO2 concentrations and the incidence of bloater defect were also followed. The enumeration of Enterobacteriaceae on Violet Red Bile Glucose agar plates detected a cell density of 5.2 ± 0.7 log CFU/g on day 2, which declined to undetectable levels by day 8. A metagenomic analysis identified Leuconostocaceae in all fermentations at 10 to 62%. The presence of both bacterial families in fermentations brined with CaCl2 and NaCl coincided with a bloater index of 24.0 ± 10.3 to 58.8 ± 23.9. The prevalence of Lactobacillaceae in a cucumber fermentation brined with NaCl with a bloater index of 41.7 on day 5 suggests a contribution to bloater defect. This study identifies the utilization of sugars and malic acid by the cucumber indigenous Lactobacillaceae, Leuconostocaceae and Enterobacteriaceae as potential contributors to CO2 production during cucumber fermentation and the consequent bloater defect.


Asunto(s)
Dióxido de Carbono/análisis , Cucumis sativus/microbiología , Enterobacteriaceae/metabolismo , Lactobacillaceae/metabolismo , Leuconostocaceae/metabolismo , Cloruro de Calcio , Fermentación , Concentración de Iones de Hidrógeno , Malatos/metabolismo , Microbiota/fisiología , Sales (Química) , Cloruro de Sodio/análisis
11.
Bioresour Technol ; 330: 124948, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33735731

RESUMEN

This study was aimed at developing a compound microbial agent to facilitate the composting of Chinese medicinal herbal residues (CMHRs). Different microbial agents were inoculated into a mixture of CMHRs, sheep manure, and biochar and composted for 42 days. The results demonstrated that the compound microbial agent (K1 + P1 + N4) inoculated in T1 can extend the thermophilic period during composting. Compared with control check (CK), the total nutrient contents of the vaccinated groups increased by 5.45-28.54%, and T1 had the highest value. The total organic carbon degradation rate and germination index of T1 were 1.95 and 1.03 times higher than those of the control. Moreover, significant increases in the abundance and diversity of the microbial community were also found in T1. Consequently, using K1 + P1 + N4 as a microbial agent for the composting of CMHRs is recommended, and this research provides a new idea to solve the problem of waste of CMHRs.


Asunto(s)
Compostaje , Microbiota , Animales , China , Estiércol , Nitrógeno/análisis , Ovinos , Suelo
12.
Bioresour Technol ; 330: 124945, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33735733

RESUMEN

The influence of copper ion (Cu2+) on anaerobic ammonium oxidation (anammox) performance and microbial community structures after oxytetracycline (OTC) stress recovery were assessed. Experimental results demonstrated that anammox performance were stressed by 1.0 mg L-1 Cu2+ and inhibitions were reversible with total nitrogen removal rate higher than 3.08 ± 0.2 kg N m-3 d-1. The residual OTC in the anammox sludge could combine with Cu2+ introduced and thereby retarded inhibition on performance in the presence of 2.0 mg L-1 Cu2+. Moreover, the positive relation of dominant bacterium Ca. Anammoxoglobus with the abundance of functional genes and parts of antibiotic resistance genes were observed, suggesting that regain of performance was the results of the gradual domestication of latent resistant species after inhibition. This investigation reveals new insights into resistance of anammox performance for Cu2+ and OTC.


Asunto(s)
Microbiota , Oxitetraciclina , Anaerobiosis , Antibacterianos/farmacología , Reactores Biológicos , Cobre , Farmacorresistencia Microbiana/genética , Nitrógeno , Oxidación-Reducción , Oxitetraciclina/farmacología , Aguas del Alcantarillado
13.
Bioresour Technol ; 330: 124954, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33740583

RESUMEN

Polyethylene glycol-600 (PEG-600), as a carrier for slow release of organic substances, can improve the biocompatibility of packing fillers and the construction of biofilms. The gradient experiments were established to evaluate the feasibility of adding different content of PEG-600 to the biofilter for enhancing toluene removal. In particular, the evolution trend of microbial community embedded in packing fillers was measured by 16S rRNA-based gene sequencing. Results showed that the toluene removal efficiency of biofilter with 7.5% adding content of the PEG-600 was greatly improved, and the maximum elimination capacity of 152 g/(m3·h) was obtained. The introduction of PEG-600 enhanced the tolerance ability to withstand the transient impact loading and intensified the production of extracellular polymeric substances and bonding strength of biofilms. It should be noted that the abundance of Pseudomonas and Steroidobacter at genus level increased significantly. The microbial community evolved into a co-degradation system of toluene and PEG-600.


Asunto(s)
Contaminantes Atmosféricos , Microbiota , Biodegradación Ambiental , Filtración , Nutrientes , Polietilenglicoles , ARN Ribosómico 16S/genética , Tolueno
14.
Bioresour Technol ; 330: 124968, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33744733

RESUMEN

The down flow structured bed bioreactor (DFSBR) was applied to treat synthetic acid mine drainage (AMD) to reduce sulfate, increase the pH and precipitate metals in solutions (Co, Cu, Fe, Mn, Ni and Zn) using vinasse as an electron donor for sulfate-reducing bacteria (SRB). DFSBR achieved sulfate removal efficiencies between 55 and 91%, removal of Co and Ni were obtained with efficiencies greater than 80%, while Fe, Zn, Cu and Mn were removed with average efficiencies of 70, 80, 73 and 60%, respectively. Sulfate reduction increased pH from moderately acidic to 6.7-7.5. Modelling data confirmed the experimental results and metal sulfide precipitation was the mainly responsible for metal removal. The main genera responsible for sulfate and metal reduction were Geobacter and Desulfovibrio while fermenters were Parabacteroides and Sulfurovum. Moreover, in syntrophism with SRB, they played an important role in the efficiency of metal and sulfate removal.


Asunto(s)
Microbiota , Saccharum , Reactores Biológicos , Electrones , Concentración de Iones de Hidrógeno , Sulfatos
15.
Bioresour Technol ; 330: 124998, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33757679

RESUMEN

Renewable energy, water conservation, and environmental protection are the most important challenges today. Osmotic membrane bioreactor (OMBR) is an innovative process showing superior performance in bioenergy production, eliminating contaminants, and low fouling tendency. However, salinity build-up is the main drawback of this process. Identifying the microbial community can improve the process in bioenergy production and contaminant treatment. This review aims to study the recent progress and challenges of OMBRs in contaminant removal, microbial communities and bioenergy production. OMBRs are widely reported to remove over 80% of total organic carbon, PO43-, NH4+ and emerging contaminants from wastewater. The most important microbial phyla for both hydrogen and methane production in OMBR are Firmicutes, Proteobacteria and Bacteroidetes. Firmicutes' dominance in anaerobic processes is considerably increased from usually 20% at the beginning to 80% under stable condition. Overall, OMBR process has great potential to be applied for simultaneous bioenergy production and wastewater treatment.


Asunto(s)
Microbiota , Purificación del Agua , Reactores Biológicos , Membranas Artificiales , Ósmosis , Aguas Residuales
16.
Bioresour Technol ; 330: 124983, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33761451

RESUMEN

Sludge reduction is considered a main target for sludge treatment and an urgent issue for wastewater treatment. In this study, two dominant sludge-degrading strains, identified as Chryseobacterium sp. B4 and Serratia sp. H1, were used for inoculation in swine wastewater treatment to investigate the enhancement of sludge reduction. The results showed the volatile suspended solid (VSS) removal rate in experimental groups inoculated with Chryseobacterium sp. B4, Serratia sp. H1, and a combination of the two strains improved by 49.4%, 11.0%, and 30.5%, compared with the control with no inoculation. Furthermore, microbial community structure and functional prediction analyses indicated Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria could play an essential role in sludge reduction, and the dominant sludge-degrading strains B4 and H1 enhanced sludge reduction by strengthening carbohydrate, nucleotide, amino acid, and lipid metabolism and membrane transport functions. This study provides new insights into sludge reduction during wastewater treatment with dominant sludge-degrading strains.


Asunto(s)
Chryseobacterium , Microbiota , Purificación del Agua , Animales , Reactores Biológicos , Serratia , Aguas del Alcantarillado , Porcinos , Aguas Residuales
17.
Bioresour Technol ; 330: 125001, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33773269

RESUMEN

Anaerobic digestion is a long-established technology for the valorization of diverse organic wastes with concomitant generation of valuable resources. However, mono-digestion (i.e., anaerobic digestion using one feedstock) suffers from challenges associated with feedstock characteristics. Co-digestion using multiple feedstocks provides the potential to overcome these limitations. Significant research and development efforts have highlighted several inherent merits of co-digestion, including enhanced digestibility due to synergistic effects of co-substrates, better process stability, and higher nutrient value of the produced co-digestate. However, studies focused on the underlying effects of diverse co-feedstocks on digester performance and stability have not been synthesized so far. This review fills this gap by highlighting the limitations of mono-digestion and critically examining the benefits of co-digestion. Furthermore, this review discusses synergistic effect of co-substrates, characterization of microbial communities, the prediction of biogas production via different kinetic models, and highlights future research directions for the development of a sustainable biorefinery.


Asunto(s)
Reactores Biológicos , Microbiota , Anaerobiosis , Biocombustibles , Digestión , Metano
18.
Nat Commun ; 12(1): 1498, 2021 03 08.
Artículo en Inglés | MEDLINE | ID: mdl-33686084

RESUMEN

Sugarcane ethanol fermentation represents a simple microbial community dominated by S. cerevisiae and co-occurring bacteria with a clearly defined functionality. In this study, we dissect the microbial interactions in sugarcane ethanol fermentation by combinatorically reconstituting every possible combination of species, comprising approximately 80% of the biodiversity in terms of relative abundance. Functional landscape analysis shows that higher-order interactions counterbalance the negative effect of pairwise interactions on ethanol yield. In addition, we find that Lactobacillus amylovorus improves the yeast growth rate and ethanol yield by cross-feeding acetaldehyde, as shown by flux balance analysis and laboratory experiments. Our results suggest that Lactobacillus amylovorus could be considered a beneficial bacterium with the potential to improve sugarcane ethanol fermentation yields by almost 3%. These data highlight the biotechnological importance of comprehensively studying microbial communities and could be extended to other microbial systems with relevance to human health and the environment.


Asunto(s)
Fenómenos Fisiológicos Bacterianos , Etanol/metabolismo , Fermentación , Interacciones Microbianas/fisiología , Saccharomyces cerevisiae/fisiología , Acetaldehído/metabolismo , Acetaldehído/farmacología , Bacterias/clasificación , Bacterias/crecimiento & desarrollo , Biodiversidad , Microbiología Industrial/métodos , Lactobacillus/metabolismo , Microbiota , Melaza , Saccharomyces cerevisiae/efectos de los fármacos , Saccharum
19.
Sci Total Environ ; 772: 145674, 2021 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-33663956

RESUMEN

Soil serves many important ecological functions and is an integral part of our existence as a society. However, concerns for soil health are growing globally, in part due to the negative impacts of agricultural management on soil resources. The production of perennial bioenergy crops on marginal land in row-crop production systems is one solution that could improve land-use efficiency and address the sustainability of cropland management. Because the relationship between crop management and the environment is complex, more research is needed to evaluate the potential benefits perennial bioenergy crop production has on soil health, as well as other ecosystem services. In this study, shrub willow buffers were strategically integrated into a corn-soybean cropping system with the main objective of reducing nitrate-N leaching from grain crop production while producing biomass for bioenergy. Two buffer systems (defined by landscape positions) were included for comparison, one on marginal land with exposure to nitrate-N leaching from upslope grain (southern plots) and one on fertile soils with less nitrate-N leaching potential (northern plots). Evaluation of soil (chemistry, bulk density, microbial community) and shrub willow vegetation properties (fine roots, leaf litter decomposition, and nutrient uptake dynamics), showed that landscape position plays an important role in (1) the dynamics of soil chemical properties, (2) shrub willow's influence and productivity, and (3) the provision of additional ecosystem services such as reductions in nitrous oxide emissions and nitrate-N leaching. In addition, the combination of crop type and landscape position (N-grain, N-willow, S-grain, and S-willow) influenced the species composition of the soil microbial community, resulting in unique and identifiable communities. These results highlight the potential application of shrub willow buffers for ecosystem service provision and support of ecosystem processes; however, understanding the relationship between the microbial community, crop type, and landscape is important for understanding the sustainability of the design.


Asunto(s)
Microbiota , Salix , Illinois , Suelo , Soja , Zea mays
20.
BMC Plant Biol ; 21(1): 127, 2021 Mar 04.
Artículo en Inglés | MEDLINE | ID: mdl-33663379

RESUMEN

BACKGROUND: Dendrobium is a precious herbal that belongs to Orchidaceae and is widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most research still focuses on endophytes, and there is still large amount unknown about rhizosphere microorganisms. To investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during the maturity stage of three kinds of Dendrobium species. RESULTS: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Gemmatimonadetes. And through WGCNA analysis, we found the hub flora was also belong to Acidobacteria, Actinobacteria and Proteobacteria. CONCLUSIONS: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and that the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, and Bacteroidetes. Moreover, the smaller the bacterial level, the greater the difference among Dendrobium species. These results fill knowledge gaps in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.


Asunto(s)
Bacterias/aislamiento & purificación , Dendrobium/microbiología , Plantas Medicinales/microbiología , Rizosfera , Microbiología del Suelo , Bacterias/clasificación , Biodiversidad , Medicamentos Herbarios Chinos , Secuenciación de Nucleótidos de Alto Rendimiento , Microbiota
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