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1.
Monaldi Arch Chest Dis ; 90(2)2020 May 07.
Artículo en Inglés | MEDLINE | ID: mdl-32380802

RESUMEN

A novel coronavirus, SARS-CoV-2, thought to have originated from bats causes COVID-19 infection which was first reported from Wuhan, China in December 2019. This virus has a high infectivity rate and has impacted a significant chunk of the population worldwide. The spectrum of disease ranges from mild to severe with respiratory system being the most commonly affected. Cardiovascular system often gets involved in later stages of the disease with acute cardiac injury, heart failure and arrhythmias being the common complications. In addition, the presence of cardiovascular co-morbidities such as hypertension, coronary artery disease in these patients are often associated with poor prognosis. It is still not clear regarding the exact mechanism explaining cardiovascular system involvement in COVID-19. Multiple theories have been put forward however, more robust studies are required to fully elucidate the "heart and virus" link. The disease has already made its presence felt on the global stage and its impact in the developing countries is going to be profound. These nations not only have a poorly developed healthcare system but there is also a huge burden of cardiovascular diseases. As a result, COVID-19 would adversely impact the already overburdened healthcare network leading to impaired cardiovascular care delivery especially for acute coronary syndrome and heart failure patients.


Asunto(s)
Enfermedades Cardiovasculares/complicaciones , Sistema Cardiovascular/virología , Infecciones por Coronavirus/complicaciones , Neumonía Viral/complicaciones , Inhibidores de la Enzima Convertidora de Angiotensina/uso terapéutico , Arritmias Cardíacas/virología , Betacoronavirus , Enfermedades Cardiovasculares/virología , Comorbilidad , Países en Desarrollo , Corazón/virología , Trasplante de Corazón , Humanos , Miocarditis/virología , Miocardio/patología , Pandemias , Infarto del Miocardio con Elevación del ST/virología
2.
Life Sci ; 251: 117644, 2020 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-32259604

RESUMEN

AIMS: Electronic cigarette (ECIG) has been used as an alternative to tobacco smoking as it lacks the majority of toxicants found in tobacco smoke. However, the effect of ECIG aerosol inhalation on cardiac health are not well studied. The present study aimed to compare the effects of ECIGs with that of combustible tobacco cigarette (T-Cigs) and waterpipe (WP) smoke on cardiac biomarkers of oxidative stress, inflammation, and fibrosis. MAIN METHODS: Rats were randomized into control (fresh air, n = 12), ECIG aerosol (n = 12), T-Cig smoke (n = 15), or WP (n = 13) smoke conditions in which they were exposed 1 h/daily, 6 day/week for 4 weeks. Cardiac biomarkers of oxidative stress, inflammation, and remodeling were assessed. KEY FINDINGS: Relative to control, significant increase in heart to body weight ratio was observed in all exposed groups. Cardiac endothelin-1 and myeloperoxidase were increased for ECIG and T-Cig. Cardiac nitrite and TBARS were increased in all exposed groups, but activity of superoxide dismutase was increased for ECIG and T-Cig only while glutathione levels increased for ECIG only. No changes were observed for cardiac C-reactive protein and catalase activity. Cardiac fibrosis was observed in all exposed groups coupled with an increase in the transforming growth factor beta protein that was significant for ECIG only. SIGNIFICANCE: ECIG aerosol may promote cardiac alterations in similar manner to tobacco smoke by promoting myocardial oxidative stress and inflammation leading to fibrosis. With regard to cardiac health, exposure to ECIG aerosol and combustible T-Cig smoke may lead to similar adverse outcomes.


Asunto(s)
Sistemas Electrónicos de Liberación de Nicotina , Inflamación/etiología , Miocardio/patología , Humo/efectos adversos , Contaminación por Humo de Tabaco/efectos adversos , Aerosoles , Animales , Fumar Cigarrillos/efectos adversos , Fibrosis/etiología , Inflamación/patología , Masculino , Estrés Oxidativo , Distribución Aleatoria , Ratas , Ratas Wistar , Vapeo/efectos adversos , Fumar en Pipa de Agua/efectos adversos
3.
Chin J Nat Med ; 18(3): 226-233, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32245593

RESUMEN

Shenfu injection (SFI), a Chinese medicinal product, shows potent efficacy in treating sepsis. The aim of the present study was to clarify the protective effects of SFI against lipopolysaccharide (LPS)-induced myocardial inflammation and apoptosis. Experiments were carried out in Sprague-Dawley (SD) rats treated with LPS or LPS + SFI, and in H9C2 cardiomyocytes. The sepsis-associated myocardial inflammation and apoptosis was induced by the intraperitoneal injection of LPS (20 mg·kg-1). SFI attenuated the increased expression of tumor necrosis factor (TNF)-α and interleukin (IL)-1ß induced by LPS both in serum and heart. In LPS group, cell viability was reduced, and reversed after SFI administration. LPS treatment increased the expression levels of cleaved-caspase 3 and Bax, and those of Bcl2 and Bcl2/Bax. These two trends were reversed by SFI administration. The expression levels of phosphorylated mitogen-activated protein kinase kinase (p-MEK) and phosphorylated extracellular regulated protein kinases (p-ERK) were increased by LPS, and reversed by SFI. MEK inhibitor U0126 attenuated the apoptosis induced by LPS. These results indicate that SFI could treat LPS-induced cardiac dysfunction. In conclusion, SFI attenuates the inflammation and apoptosis induced by LPS via downregulating the MEK and ERK signaling pathways.


Asunto(s)
Apoptosis/efectos de los fármacos , Medicamentos Herbarios Chinos/farmacología , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Miocarditis/tratamiento farmacológico , Miocitos Cardíacos/efectos de los fármacos , Sepsis/tratamiento farmacológico , Animales , Caspasa 3/metabolismo , Interleucina-1beta/metabolismo , Lipopolisacáridos , Masculino , Miocardio/patología , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Ratas , Ratas Sprague-Dawley , Factor de Necrosis Tumoral alfa/metabolismo , Proteína X Asociada a bcl-2/metabolismo
4.
Rev Med Liege ; 75(4): 226-232, 2020 Apr.
Artículo en Francés | MEDLINE | ID: mdl-32267110

RESUMEN

COVID-2019 disease mainly affects the respiratory tract and can progress in severe cases to pneumonia, acute respiratory distress syndrome and multi-organ failure. Patients with prior cardiovascular disease are at higher risk of developing an infection and progressing to a severe form of the disease. Also, due to the growing number of infected cases, it is clear that, in addition to the typical respiratory symptoms caused by the infection, some patients suffer from cardiovascular damage. This condition can, in fact, cause significant myocardial damage, which worsens the disease and affects the prognosis. Based on the results of currently published research, it seems important to discuss the manifestations and characteristics of myocardial damage induced by COVID-19 and its impact on patient prognosis.


Asunto(s)
Betacoronavirus , Enfermedades Cardiovasculares , Infecciones por Coronavirus , Pandemias , Neumonía Viral , Betacoronavirus/patogenicidad , Enfermedades Cardiovasculares/complicaciones , Comorbilidad , Infecciones por Coronavirus/complicaciones , Humanos , Miocardio/patología , Neumonía Viral/complicaciones , Pronóstico
5.
Medicine (Baltimore) ; 99(9): e19399, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32118793

RESUMEN

Gene expressions in the myocardium have been shown to vary between different causes of death, which can be utilized in the recognition of varied processes. Our previous work with a limited number of cases showed a high messenger ribonucleic acid expression of the transcript variant alt-a of cyclin dependent kinase inhibitor p21 (p21 alt-a) in chronic cardiac ischemia deaths and a low expression in hypothermia deaths and acute myocardial ischemia deaths. In present work, p21 alt-a expression in the myocardium of human cadavers was calculated using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. In this collection of 143 samples, the p21 alt-a expression was significantly lower in hypothermia than in chronic cardiac ischemic heart disease with (P < .001) or without (P < .001) acute myocardial infarction and in other cardiac and respiratory disease deaths (P < .000). Chronic ischemic heart disease in hypothermia cases did not increase the expression. The p21 alt-a expression did not correlate with postmortem interval, quality of RNA or with the age of the deceased. The p21 alt-a referenced to GAPDH expression in cadaver myocardium has apparent potential as a marker distinguishing between hypothermia and cardiac/respiratory diseases as causes of death.


Asunto(s)
Causas de Muerte , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/análisis , Cardiopatías/fisiopatología , Hipotermia/fisiopatología , Miocardio/patología , Finlandia , Cardiopatías/patología , Humanos , Hipotermia/patología
6.
Braz J Med Biol Res ; 53(3): e8969, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32130291

RESUMEN

This study investigated the repercussions of adjuvant-induced arthritis (AIA) on body composition and the structural organization of the soleus and cardiac muscles, including their vascularization, at different times of disease manifestation. Male rats were submitted to AIA induction by intradermal administration of 100 µL of Mycobacterium tuberculosis (50 mg/mL), in the right hind paw. Animals submitted to AIA were studied 4 (AIA4), 15 (AIA15), and 40 (AIA40) days after AIA induction as well as a control group of animals not submitted to AIA. Unlike the control animals, AIA animals did not gain body mass throughout the evolution of the disease. AIA reduced food consumption, but only on the 40th day after induction. In the soleus muscle, AIA reduced the wet mass in a time-dependent manner but increased the capillary density by the 15th day and the fiber density by both 15 and 40 days after induction. The diameter of the soleus fiber decreased from the 4th day after AIA induction as well as the capillary/fiber ratio, which was most evident on the 40th day. Moreover, AIA induced slight histopathological changes in the cardiac muscle that were more evident on the 15th day after induction. In conclusion, AIA-induced changes in body composition as well as in the soleus muscle fibers and vasculature have early onset but are more evident by the 15th day after induction. Moreover, the heart may be a target organ of AIA, although less sensitive than skeletal muscles.


Asunto(s)
Artritis Experimental/patología , Composición Corporal , Músculo Esquelético/patología , Miocardio/patología , Animales , Artritis Experimental/metabolismo , Modelos Animales de Enfermedad , Masculino , Músculo Esquelético/metabolismo , Miocardio/metabolismo , Ratas
7.
Life Sci ; 250: 117531, 2020 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-32151691

RESUMEN

AIMS: To investigate the protective effects and mechanism of semaglutide on exercise-induced myocardial injury. MAIN METHODS: Effects of semaglutide on lipopolysaccharide (LPS)-induced oxidative stress injuries and inflammatory response were assessed in H9c2 cell via MTT assay and Western blot. Quiet control group, over training group and three doses of semaglutide treated overtraining groups were subjected to the swimming training with increasing load for consecutive 10 weeks. Immediately after the last training, the body weight, myocardial morphological changes, injury markers and inflammatory response related proteins of the model rats were analyzed. KEY FINDINGS: Semaglutide at three concentrations in LPS treated H9c2 cells significantly increased the survival rate and inhibited the apoptosis of cardiomyocytes. Moreover, semaglutide activated AMPK pathway, improve autophagy and inhibited reactive oxygen species production in LPS treated H9C2 cells. In vivo results further revealed that chronic treatment of semaglutide induced significant increase in myocardial injury markers. The pathological histology analysis results showed that semaglutide ameliorated myocardial morphological changes, reduced area of lipid accumulation and significantly decreased the expression levels of NF-κB, TNF-α and IL-1ß. SIGNIFICANCE: Semaglutide exert the protective effects on exercise-induced cardiomyopathy by activating AMPK pathway, increasing autophagy, reducing the production of ROS and inflammation-related proteins.


Asunto(s)
Péptidos Similares al Glucagón/farmacología , Lesiones Cardíacas/tratamiento farmacológico , Inflamación/tratamiento farmacológico , Miocardio/patología , Estrés Oxidativo/efectos de los fármacos , Condicionamiento Físico Animal/efectos adversos , Proteínas Quinasas Activadas por AMP/metabolismo , Animales , Apoptosis , Línea Celular , Supervivencia Celular , Citocinas/metabolismo , Lesiones Cardíacas/prevención & control , Interleucina-1beta/metabolismo , Lípidos/química , Lipopolisacáridos , Masculino , FN-kappa B/metabolismo , Ratas , Ratas Sprague-Dawley , Especies Reactivas de Oxígeno/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo
8.
Life Sci ; 250: 117582, 2020 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-32222465

RESUMEN

The ineffective immunosuppressant's and targeted strategies to neutralize inflammatory mediators have worsened the scenario of heart failure and have opened many questions for debate. Stem cell therapy has proven to be a promising approach for treating heart following myocardial infarction (MI). Adult stem cells, induced pluripotent stem cells and embryonic stem cells are possible cell types and have successfully shown to regenerate damaged myocardial tissue in pre-clinical and clinical studies. Current implications of using mesenchymal stem cells (MSCs) owing to their immunomodulatory functions and paracrine effects could serve as an effective alternative treatment option for rejuvenating the heart post MI. The major setback associated with the use of MSCs is reduced cell retention, engraftment and decreased effectiveness. With a few reports on understanding the role of inflammation and its dual effects on the structure and function of heart, this review focuses on these missing insights and further exemplifies the role of MSCs as an alternative therapy in treating the pathological consequences in myocardial infarction (MI).


Asunto(s)
Inflamación/patología , Infarto del Miocardio/terapia , Miocardio/patología , Regeneración , Trasplante de Células Madre , Animales , Proliferación Celular , Activación de Complemento , Fibrosis , Humanos , Células Madre Mesenquimatosas/citología , Ratones , Neutrófilos/citología , Estrés Oxidativo , Células Madre Pluripotentes/citología , Medicina Regenerativa/métodos
9.
Zhonghua Bing Li Xue Za Zhi ; 49(5): 411-417, 2020 May 08.
Artículo en Chino | MEDLINE | ID: mdl-32172546

RESUMEN

Objective: To investigate the pathological characteristics and the clinical significance of novel coronavirus (2019-nCoV)-infected pneumonia (termed by WHO as coronavirus disease 2019, COVID-19). Methods: Minimally invasive autopsies from lung, heart, kidney, spleen, bone marrow, liver, pancreas, stomach, intestine, thyroid and skin were performed on three patients died of novel coronavirus pneumonia in Chongqing, China. Hematoxylin and eosin staining (HE), transmission electron microcopy, and histochemical staining were performed to investigate the pathological changes of indicated organs or tissues. Immunohistochemical staining was conducted to evaluate the infiltration of immune cells as well as the expression of 2019-nCoV proteins. Real time PCR was carried out to detect the RNA of 2019-nCoV. Results: Various damages were observed in the alveolar structure, with minor serous exudation and fibrin exudation. Hyaline membrane formation was observed in some alveoli. The infiltrated immune cells in alveoli were majorly macrophages and monocytes. Moderate multinucleated giant cells, minimal lymphocytes, eosinophils and neutrophils were also observed. Most of infiltrated lymphocytes were CD4-positive T cells. Significant proliferation of type Ⅱ alveolar epithelia and focal desquamation of alveolar epithelia were also indicated. The blood vessels of alveolar septum were congested, edematous and widened, with modest infiltration of monocytes and lymphocytes. Hyaline thrombi were found in a minority of microvessels. Focal hemorrhage in lung tissue, organization of exudates in some alveolar cavities, and pulmonary interstitial fibrosis were observed. Part of the bronchial epithelia were exfoliated. Coronavirus particles in bronchial mucosal epithelia and type Ⅱ alveolar epithelia were observed under electron microscope. Immunohistochemical staining showed that part of the alveolar epithelia and macrophages were positive for 2019-nCoV antigen. Real time PCR analyses identified positive signals for 2019-nCoV nucleic acid. Decreased numbers of lymphocyte, cell degeneration and necrosis were observed in spleen. Furthermore, degeneration and necrosis of parenchymal cells, formation of hyaline thrombus in small vessels, and pathological changes of chronic diseases were observed in other organs and tissues, while no evidence of coronavirus infection was observed in these organs. Conclusions: The lungs from novel coronavirus pneumonia patients manifest significant pathological lesions, including the alveolar exudative inflammation and interstitial inflammation, alveolar epithelium proliferation and hyaline membrane formation. While the 2019-nCoV is mainly distributed in lung, the infection also involves in the damages of heart, vessels, liver, kidney and other organs. Further studies are warranted to investigate the mechanism underlying pathological changes of this disease.


Asunto(s)
Infecciones por Coronavirus , Pulmón/patología , Pandemias , Neumonía Viral , Autopsia , Betacoronavirus/genética , Betacoronavirus/aislamiento & purificación , China , Infecciones por Coronavirus/patología , Humanos , Riñón/patología , Hígado/patología , Miocardio/patología , Neumonía Viral/patología , Reacción en Cadena en Tiempo Real de la Polimerasa , Piel/patología , Glándula Tiroides/patología
10.
Basic Res Cardiol ; 115(3): 24, 2020 03 05.
Artículo en Inglés | MEDLINE | ID: mdl-32140789

RESUMEN

Intramyocardial hemorrhage is an independent predictor of adverse outcomes in ST-segment elevation myocardial infarction (STEMI). Iron deposition resulting from ischemia-reperfusion injury (I/R) is pro-inflammatory and has been associated with adverse remodeling. The role of iron chelation in hemorrhagic acute myocardial infarction (AMI) has never been explored. The purpose of this study was to investigate the cardioprotection offered by the iron-chelating agent deferiprone (DFP) in a porcine AMI model by evaluating hemorrhage neutralization and subsequent cardiac remodeling. Two groups of animals underwent a reperfused AMI procedure: control and DFP treated (N = 7 each). A comprehensive MRI examination was performed in healthy state and up to week 4 post-AMI, followed by histological assessment. Infarct size was not significantly different between the two groups; however, the DFP group demonstrated earlier resolution of hemorrhage (by T2* imaging) and edema (by T2 imaging). Additionally, ventricular enlargement and myocardial hypertrophy (wall thickness and mass) were significantly smaller with DFP, suggesting reduced adverse remodeling, compared to control. The histologic results were consistent with the MRI findings. To date, there is no effective targeted therapy for reperfusion hemorrhage. Our proof-of-concept study is the first to identify hemorrhage-derived iron as a therapeutic target in I/R and exploit the cardioprotective properties of an iron-chelating drug candidate in the setting of AMI. Iron chelation could potentially serve as an adjunctive therapy in hemorrhagic AMI.


Asunto(s)
Cardiotónicos/farmacología , Deferiprona/uso terapéutico , Hemorragia/tratamiento farmacológico , Hemorragia/etiología , Quelantes del Hierro/uso terapéutico , Infarto del Miocardio/complicaciones , Miocardio/patología , Remodelación Ventricular/efectos de los fármacos , Animales , Cardiotónicos/farmacocinética , Cardiotónicos/uso terapéutico , Deferiprona/farmacocinética , Deferiprona/farmacología , Modelos Animales de Enfermedad , Femenino , Hemorragia/patología , Quelantes del Hierro/farmacocinética , Quelantes del Hierro/farmacología , Infarto del Miocardio/patología , Porcinos
11.
Braz J Med Biol Res ; 53(3): e8761, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32159612

RESUMEN

Nitric oxide (NO) inhibition by high-dose NG-nitro-L-arginine methyl ester (L-NAME) is associated with several detrimental effects on the cardiovascular system. However, low-dose L-NAME increases NO synthesis, which in turn induces physiological cardiovascular benefits, probably by activating a protective negative feedback mechanism. Aerobic exercise, likewise, improves several cardiovascular functions in healthy hearts, but its effects are not known when chronically associated with low-dose L-NAME. Thus, we tested whether the association between low-dose L-NAME administration and chronic aerobic exercise promotes beneficial effects to the cardiovascular system, evaluating the cardiac remodeling process. Male Wistar rats were randomly assigned to control (C), L-NAME (L), chronic aerobic exercise (Ex), and chronic aerobic exercise associated to L-NAME (ExL). Aerobic training was performed with progressive intensity for 12 weeks; L-NAME (1.5 mg·kg-1·day-1) was administered by orogastric gavage. Low-dose L-NAME alone did not change systolic blood pressure (SBP), but ExL significantly increased SBP at week 8 with normalization after 12 weeks. Furthermore, ExL promoted the elevation of left ventricle (LV) end-diastolic pressure without the presence of cardiac hypertrophy and fibrosis. Time to 50% shortening and relaxation were reduced in ExL, suggesting a cardiomyocyte contractile improvement. In addition, the time to 50% Ca2+ peak was increased without alterations in Ca2+ amplitude and time to 50% Ca2+ decay. In conclusion, the association of chronic aerobic exercise and low-dose L-NAME prevented cardiac pathological remodeling and induced cardiomyocyte contractile function improvement; however, it did not alter myocyte affinity and sensitivity to intracellular Ca2+ handling.


Asunto(s)
Calcio/análisis , Inhibidores Enzimáticos/farmacología , Contracción Miocárdica/efectos de los fármacos , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico Sintasa/efectos de los fármacos , Condicionamiento Físico Animal/fisiología , Adiposidad , Animales , Peso Corporal/fisiología , Inhibidores Enzimáticos/administración & dosificación , Hemodinámica , Masculino , Modelos Animales , Actividad Motora/fisiología , Miocardio/patología , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/fisiología , NG-Nitroarginina Metil Éster/administración & dosificación , Óxido Nítrico Sintasa/metabolismo , Ratas Wistar , Presión Ventricular/efectos de los fármacos
13.
Zhonghua Jie He He Hu Xi Za Zhi ; 43(3): 209-214, 2020 Mar 12.
Artículo en Chino | MEDLINE | ID: mdl-32164090

RESUMEN

Objective: To investigate the clinical characteristics of medical staff with novel coronavirus pneumonia(NCP). Methods: 30 patients infected with novel coronavirus referred to jianghan university hospital between January 11, 2020 and January 3, 2020 were studied. The data reviewed included those of clinical manifestations, laboratory investigation and Radiographic features. Results: The patients consisted of 10 men and 20 women, including 22 doctors and 8 nurses,aged 21~59 years(mean 35±8 years).They were divided to 26 common type and 4 severe cases, all of whom had close(within 1m) contact with patients infected of novel coronavirus pneumonia. The average contact times were 12 (7,16) and the average cumulative contact time was 2 (1.5,2.7) h.Clinical symptoms of these patients were fever in 23 patients (76.67%) , headache in 16 petients (53.33%) , fatigue or myalgia in 21patients (70%) , nausea, vomiting or diarrhea in 9 petients (30%) , cough in 25 petients (83.33%) , and dyspnea in 14 petients (46.67%) .Routine blood test revealed WBC<4.0×10(9)/L in 8 petients (26.67%) , (4-10) ×10(9)/L in 22 petients (73.33%) , and WBC>4.0×10(9)/L in 4 petients (13.33%) during the disease.Lymphocyte count<1.0×10(9)/L occurred in 12 petients (40%),abnormal liver function in 7 petients (23.33%) ,myocardial damage in 5 petients(16.67%), elevated D-dimer (>0.5mg/l) in 5 patients (16.67%). Compared with normal patients, the average exposure times, cumulative exposure time, BMI, Fever time, white blood cell count, liver enzyme, LDH, myoenzyme and D-dimer were significantly increased in severe patients, while the lymphocyte count and albumin levels in peripheral blood were significantly decreased.Chest CT mainly showed patchy shadows and interstitial changes.According to imaging examination, 11 patients (36.67%) showed Unilateral pneumonia and 19 patients (63.33%) showed bilateral pneumonia,4 patients (13.33%) showed bilateral multiple mottling and ground-glass opacity.Compared with the patients infected in the protected period, the proportion of severe infection and bilateral pneumonia were both increased in the patients infected in unprotected period. Conclusion: Medical staffs are at higher risk of infection.Infection rates are associated with contact time, the amount of suction virus. Severe patients had BMI increased, heating time prolonged, white blood cell count, lymphocyte count, D-dimer and albumin level significantly changed and were prone to be complicated with liver damage and myocardial damage.Strict protection measures is important to prevent infection for medical workers.


Asunto(s)
Betacoronavirus/patogenicidad , Infecciones por Coronavirus , Fiebre , Personal de Salud , Transmisión de Enfermedad Infecciosa de Paciente a Profesional , Neumonía Viral , Adulto , Índice de Masa Corporal , Infecciones por Coronavirus/complicaciones , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/transmisión , Tos/etiología , Disnea/etiología , Femenino , Fiebre/clasificación , Fiebre/etiología , Productos de Degradación de Fibrina-Fibrinógeno/análisis , Humanos , Recuento de Leucocitos , Hígado/patología , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , Miocardio/patología , Neumonía Viral/complicaciones , Neumonía Viral/diagnóstico , Neumonía Viral/transmisión , Estudios Retrospectivos , Factores de Riesgo , Albúmina Sérica , Factores de Tiempo , Adulto Joven
15.
Am Heart J ; 222: 26-29, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32004797

RESUMEN

Atrial arrhythmias commonly occur in patients with cardiac amyloidosis (CA), but there is limited data on safety or efficacy of cardioversion (DCCV) for management of these rhythms in CA. We identified 25 patients with CA (20 with transthyretin (TTR) and 5 with light-chain (AL) amyloidosis) at Duke University who underwent DCCV for atrial arrhythmias and documented procedural success, complications, and long-term morbidity and mortality. While DCCV successfully restored sinus rhythm in 96% of patients, 36% of patients experienced immediate procedural complications (primarily bradycardia and hypotension), 80% had recurrence of atrial arrhythmias at 1 year, and 52% died at 3 years, highlighting short-term safety concerns, long-term inefficacy, and poor prognosis associated with symptomatic atrial arrhythmias requiring DCCV in CA.


Asunto(s)
Amiloidosis/complicaciones , Fibrilación Atrial/terapia , Aleteo Atrial/terapia , Cardiomiopatías/complicaciones , Cardioversión Eléctrica/métodos , Anciano , Amiloidosis/diagnóstico , Fibrilación Atrial/epidemiología , Fibrilación Atrial/etiología , Aleteo Atrial/epidemiología , Aleteo Atrial/etiología , Biopsia , Cardiomiopatías/diagnóstico , Causas de Muerte/tendencias , Femenino , Estudios de Seguimiento , Humanos , Imagen por Resonancia Cinemagnética/métodos , Masculino , Morbilidad/tendencias , Miocardio/patología , Pronóstico , Estudios Retrospectivos , Factores de Riesgo , Tasa de Supervivencia/tendencias , Estados Unidos/epidemiología
16.
Nat Cell Biol ; 22(2): 246-256, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-32015438

RESUMEN

The Hippo and mammalian target of rapamycin complex 1 (mTORC1) pathways are the two predominant growth-control pathways that dictate proper organ development. We therefore explored potential crosstalk between these two functionally relevant pathways to coordinate their growth-control functions. We found that the LATS1 and LATS2 kinases, the core components of the Hippo pathway, phosphorylate S606 of Raptor, an essential component of mTORC1, to attenuate mTORC1 activation by impairing the interaction of Raptor with Rheb. The phosphomimetic Raptor-S606D knock-in mutant led to a reduction in cell size and proliferation. Compared with Raptor+/+ mice, RaptorD/D knock-in mice exhibited smaller livers and hearts, and a significant inhibition of elevation in mTORC1 signalling induced by Nf2 or Lats1 and Lats2 loss. Thus, our study reveals a direct link between the Hippo and mTORC1 pathways to fine-tune organ growth.


Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Diana Mecanicista del Complejo 1 de la Rapamicina/genética , Proteínas Serina-Treonina Quinasas/genética , Proteína Homóloga de Ras Enriquecida en el Cerebro/genética , Proteína Reguladora Asociada a mTOR/genética , Proteínas Supresoras de Tumor/genética , Animales , Sistemas CRISPR-Cas , Neoplasias del Colon/genética , Neoplasias del Colon/metabolismo , Neoplasias del Colon/patología , Femenino , Edición Génica , Células HCT116 , Células HEK293 , Células HeLa , Xenoinjertos , Humanos , Hígado/anomalías , Hígado/metabolismo , Células MCF-7 , Diana Mecanicista del Complejo 1 de la Rapamicina/metabolismo , Ratones , Ratones Desnudos , Ratones Transgénicos , Miocardio/metabolismo , Miocardio/patología , Neurofibromina 2/deficiencia , Neurofibromina 2/genética , Tamaño de los Órganos , Proteínas Serina-Treonina Quinasas/deficiencia , Proteínas Serina-Treonina Quinasas/metabolismo , Proteína Homóloga de Ras Enriquecida en el Cerebro/metabolismo , Proteína Reguladora Asociada a mTOR/metabolismo , Transducción de Señal , Proteínas Supresoras de Tumor/deficiencia
17.
PLoS One ; 15(2): e0228860, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32032383

RESUMEN

Several diseases are associated with excess of adipose tissue, and obesity is considered an independent risk factor for the development of cardiac remodeling and heart failure. Dietary aspects have been studied to elucidate the mechanisms involved in these processes. Thus, the purpose was the development and characterization of an obesity experimental model from hypercaloric diets, which resulted in cardiac remodeling and predisposition to heart failure. Thirty- day-old male Wistar rats (n = 52) were randomized into four groups: control (C), high sucrose (HS), high-fat (HF) and high-fat and sucrose (HFHS) for 20 weeks. General characteristics, comorbidities, weights of the heart, left (LV) and right ventricles, atrium, and relationships with the tibia length were evaluated. The LV myocyte cross sectional area and fraction of interstitial collagen were assayed. Cardiac function was determined by hemodynamic analysis and the contractility by cardiomyocyte contractile function. Heart failure was analyzed by pulmonary congestion, right ventricular hypertrophy, and hemodynamic parameters. HF and HFHS models led to obesity by increase in adiposity index (C = 8.3 ± 0.2% vs. HF = 10.9 ± 0.5%, HFHS = 10.2 ± 0.3%). There was no change in the morphological parameters and heart failure signals. HF and HFHS caused a reduction in times to 50% relaxation without cardiomyocyte contractile damage. The HS model presented cardiomyocyte contractile dysfunction visualized by lower shortening (C: 8.34 ± 0.32% vs. HS: 6.91 ± 0.28), as well as the Ca2+ transient amplitude was also increased when compared to HFHS. In conclusion, the experimental diets based on high amounts of sugar, lard or a combination of both did not promote cardiac remodeling with predisposition to heart failure under conditions of obesity or excess sucrose. Nevertheless, excess sucrose causes cardiomyocyte contractility dysfunction associated with alterations in the myocyte sensitivity to intracellular Ca2+.


Asunto(s)
Dieta de Carga de Carbohidratos/efectos adversos , Sacarosa en la Dieta/administración & dosificación , Sacarosa en la Dieta/efectos adversos , Insuficiencia Cardíaca/etiología , Animales , Señalización del Calcio , Colágeno/metabolismo , Dieta Alta en Grasa/efectos adversos , Grasas de la Dieta/administración & dosificación , Grasas de la Dieta/efectos adversos , Modelos Animales de Enfermedad , Ingestión de Energía , Insuficiencia Cardíaca/patología , Insuficiencia Cardíaca/fisiopatología , Masculino , Modelos Cardiovasculares , Contracción Miocárdica , Miocardio/metabolismo , Miocardio/patología , Miocitos Cardíacos/fisiología , Obesidad/complicaciones , Obesidad/patología , Obesidad/fisiopatología , Ratas , Ratas Wistar , Remodelación Ventricular/fisiología
18.
PLoS One ; 15(2): e0228736, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32053651

RESUMEN

In all patients with ST-segment elevation myocardial infarction, risk stratification should be performed before discharge. The measurement of therapy efficiency with magnetic resonance imaging has been proposed as part of the risk assessment, but it has not been adopted widely. This meta-analysis was conducted to summarize published data on the prognostic value of the proportion of salvaged myocardium inside previously ischemic myocardium (myocardial salvage index) measured by T2-weighted and T1-weighted late gadolinium enhancement magnetic resonance imaging after ST-segment elevation myocardial infarction. Random and mixed effects models were used for analyzing the data of 10 studies with 2,697 patients. The pooled myocardial salvage index, calculated as the proportion of non-necrotic myocardium inside edematous myocardium measured by T2-weighted and T1-weighted late gadolinium enhancement MRI, was 43.0% (95% confidence interval: 37.4, 48.6). The pooled length of follow-up was 12.3 months (95% confidence interval: 7.0, 17.6). The pooled incidence of major cardiac events during follow-up, defined as cardiac death, nonfatal myocardial infarction, or admission for heart failure, was 10.6% (95% confidence interval: 5.7, 15.5). The applied mixed effects model showed an absolute decrease of 1.7% in the incidence of major cardiac events during follow-up (95% confidence interval: 1.6, 1.9) with every 1% of increase in the myocardial salvage index. The heterogeneity between studies was considerable (τ = 21.3). Analysis of aggregated follow-up data after ST-segment elevation myocardial infarction suggests that the myocardial salvage index measured by T2-weighted and T1-weighted late gadolinium enhancement magnetic resonance imaging provides prognostic information on the risk of major cardiac events, but considerable heterogeneity exists between studies.


Asunto(s)
Medios de Contraste/química , Gadolinio/química , Imagen por Resonancia Magnética , Infarto del Miocardio con Elevación del ST/patología , Humanos , Miocardio/patología , Pronóstico , Factores de Riesgo , Infarto del Miocardio con Elevación del ST/diagnóstico por imagen , Índice de Severidad de la Enfermedad
19.
Biochemistry (Mosc) ; 85(Suppl 1): S20-S33, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-32087052

RESUMEN

The review is devoted to tropomyosin (Tpm) - actin-binding protein, which plays a crucial role in the regulation of contraction of skeletal and cardiac muscles. Special attention is paid to myopathies and cardiomyopathies - severe hereditary diseases of skeletal and cardiac muscles associated with point mutations in Tpm genes. The current views on the molecular mechanisms of these diseases and the effects of such mutations on the Tpm structure and functions are considered in detail. Besides, some part of the review is devoted to analysis of the properties of Tpm homodimers and heterodimers with myopathic substitutions of amino acid residues in only one of the two chains of the Tpm dimeric molecule.


Asunto(s)
Cardiomiopatías/genética , Músculo Esquelético/patología , Enfermedades Musculares/genética , Miocardio/patología , Mutación Puntual , Tropomiosina/genética , Tropomiosina/metabolismo , Actinas/metabolismo , Animales , Dimerización , Heterocigoto , Humanos , Modelos Moleculares , Contracción Muscular/genética , Unión Proteica , Isoformas de Proteínas , Tropomiosina/química
20.
PLoS One ; 15(1): e0227449, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32004354

RESUMEN

The SOD3 variant, SOD3R213G, results from substitution of arginine to glycine at amino acid 213 (R213G) in its heparin binding domain (HBD) and is a common genetic variant, reported to be associated with ischemic heart disease. However, little is understood about the role of SOD3R213G in innate immune function, and how it leads to dysfunction of the cardiovascular system. We observed pathologic changes in SOD3R213G transgenic (Tg) mice, including cystic medial degeneration of the aorta, heart inflammation, and increased circulating and organ infiltrating neutrophils. Interestingly, SOD3R213G altered the profile of SOD3 interacting proteins in neutrophils in response to G-CSF. Unexpectedly, we found that G-CSF mediated tyrosine phosphatase, SH-PTP1 was down-regulated in the neutrophils of SOD3R213G overexpressing mice. These effects were recovered by reconstitution with Wt SOD3 expressing bone marrow cells. Overall, our study reveals that SOD3R213G plays a crucial role in the function of the cardiovascular system by controlling innate immune response and signaling. These results suggest that reconstitution with SOD3 expressing bone marrow cells may be a therapeutic strategy to treat SOD3R213G mediated diseases.


Asunto(s)
Infiltración Neutrófila/fisiología , Neutrófilos/metabolismo , Superóxido Dismutasa/metabolismo , Animales , Aorta/metabolismo , Aorta/patología , Células de la Médula Ósea/citología , Células de la Médula Ósea/metabolismo , Proliferación Celular , Modelos Animales de Enfermedad , Regulación hacia Abajo , Factor Estimulante de Colonias de Granulocitos/metabolismo , Cardiopatías/inmunología , Cardiopatías/metabolismo , Cardiopatías/patología , Inmunidad Innata , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Mutagénesis Sitio-Dirigida , Miocardio/metabolismo , Miocardio/patología , Neutrófilos/citología , Proteína Tirosina Fosfatasa no Receptora Tipo 6/metabolismo , Receptores CCR2/metabolismo , Transducción de Señal , Superóxido Dismutasa/genética
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