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1.
Mutat Res ; 863-864: 503304, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33678241

RESUMEN

Presently, over 135 thousand barrels of crude oil are extracted daily from the Al-ahdeb oil fields (situated at 180 km south east of Baghdad, Iraq). Millions of gallons of untreated toxic wastes, gas and crude oil are released into the environment. Oil mining mediated pollution may cause damage to humans and the environment. To investigate potential health risks for children living in this area, we recruited a sample of 6-8 year old school children residing within a 5 km radius around the oil field, and a control sample of children of the same age from a school 40 km away from the oil-field. Exfoliated buccal mucosa cells obtained from these children were analyzed applying the micronucleus (MN) cytome assay. Possible confounding variables such as x-rays performed during three weeks preceding sampling were obtained by a questionnaire. Nuclear anomalies were significantly elevated in children living near the oil-field. Micronucleated cells 1.3-fold, nuclear buds 3-folds, binucleated cells 3-folds, karyorrhetic cells 4-folds and karyolysis more than 10 folds with a significant difference (P < 0.05). Hence, children living in the proximity of Iraqi oil-fields are at elevated risk of genetic damage, which can cause detrimental health effects in the future.


Asunto(s)
Contaminantes Atmosféricos/toxicidad , Núcleo Celular , Daño del ADN , Mucosa Bucal , Yacimiento de Petróleo y Gas , Niño , Femenino , Humanos , Masculino , Pruebas de Micronúcleos , Instituciones Académicas
2.
J Oral Facial Pain Headache ; 35(1): 54-61, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33730127

RESUMEN

AIMS: To clarify the mechanisms of hypersensitivity and spontaneous pain in intraoral structures in rats with diabetes mellitus (DM) accompanied by reduced saliva. METHODS: Adult male Sprague-Dawley rats received a single injection of streptozocin (50 mg/kg) to induce DM. Saliva volume, intraoral hypersensitivity to menthol and capsaicin solutions, and head-withdrawal thresholds (HWTs) to noxious heat and mechanical stimulation of the tongue and whisker pad were measured. RESULTS: On day 7 after streptozocin injection, rats with DM had a significantly reduced spontaneous saliva volume, polydipsia, capsaicin aversion of the intraoral mucosa, and a reduced HWT to noxious mechanical stimulation of the whisker pad skin. The HWT to noxious mechanical stimulation of the tongue reduced further on day 14 after streptozocin injection. These symptoms are similar to the orofacial and intraoral complaints of patients with DM. Meanwhile, reduction of HWT to noxious heat stimulation of the tongue and whisker pad were not observed. These results indicate that spontaneous intraoral mucosal pain and mechanical facial hypersensitivity are antecedent symptoms before mechanical hypersensitivity of the tongue. CONCLUSION: The mechanisms of saliva reduction, spontaneous intraoral mucosa pain, and mechanical hypersensitivity of intraoral and facial structures induced by DM involve both peripheral and autonomic neuropathies. Tongue hypersensitivity to noxious mechanical stimulation might be aggravated by xerostomia.


Asunto(s)
Diabetes Mellitus , Xerostomía , Animales , Humanos , Hiperalgesia , Masculino , Mucosa Bucal , Ratas , Ratas Sprague-Dawley , Saliva , Lengua , Xerostomía/inducido químicamente
3.
Swiss Dent J ; 131(3): 253-255, 2021 Mar 08.
Artículo en Inglés | MEDLINE | ID: mdl-33666388

RESUMEN

In addition to the tasks of pre-digestion, buffering of food acids, protection and remineralisation of tooth substance and moistening of the oral mucosa, saliva also plays an important role in wound healing, and a reduced flow rate represents a serious problem for patients. The saliva flow rate measurement (sialometry) on patients before radiation therapy should take place as a routine procedure, the costs are covered by the statutory health insurance. Due to the increased risk of caries in patients with reduced salivary flow, optimum oral hygiene and professional prophylactic care are crucial. In addition to nutritional guidance of the patient, saliva substitutes and medicinal salivary stimulants are available on the market. Saliva analysis has recently become more of public interest, especially in the field of personalized medicine as various drugs, viruses and DNA can be detected in saliva.


Asunto(s)
Caries Dental , Diente , Humanos , Mucosa Bucal , Higiene Bucal , Saliva
4.
Georgian Med News ; (310): 48-54, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33658409

RESUMEN

Aim of the study -to determine the indices of local immunity in patients who had combined dental pathology (caries) and gastroesophageal reflux disease. In total were exanimated 33 patients with dental caries in age from 18 to 25 years, including 21 men and 12 women. The main group consisted of 17 patients who had a combined dental pathology (caries) and gastroesophageal reflux disease. The comparison group consisted of 16 people who had dental caries without other systemic diseases.  All patients, who were included in the study was carried out the following researches: a study of the dental status, an immunological study for all patients with the determination of a quantitative assessment of the main populations and subpopulations of lymphocytes, determination of their functional activity, determination of level of serum immunoglobulins, determination of the concentration of circulating immune complexes of various molecular sizes, phagocytic activity of neutrophils and cytokine status in serum and oral fluid, as well as assessment of the course of gastroesophageal reflux disease with questionnaires. The results of the values of the resistance of hard tissues to the effects of cariogenic factors had a high positive correlation with the intensity of dental caries lesions. That confirms the presence of a relationship between manifestations of systemic diseases together with the development of dental caries, especially against the background of a decrease in caries resistance. In the presence of gastroesophageal reflux disease in patients dental caries detected spontaneous activation of lymphocyte proliferation by 25.8%, an increasing of the phagocytic activity of neutrophils, detected significantly higher indicators of the phagocytic number and phagocytic index, increased content of medium and small molecular circulating immune complexes with pathogenic properties. Detected significantly higher content of pro-inflammatory cytokines - tumor necrosis factor-α, an increased concentration of IgG as a result of antigenic stimulation, and also a significantly lower concentration of secretory IgA. It has been established that in patients with gastroesophageal reflux disease combined with dental caries observing changes in the immune system, which bear the features of subclinical immune inflammation. The study revealed a higher intensity of the carious process in patients with gastroesophageal reflux disease, which is associated with permanent acid damage of the oral mucosa. Furthermore, it was found violation in terms of concentration with increasing local immunity oral liquid proinflammatory factors and a reduced concentration of secretory IgA namely with concomitant pathologies of the gastroesophageal reflux disease.


Asunto(s)
Caries Dental , Reflujo Gastroesofágico , Adolescente , Adulto , Citocinas , Caries Dental/complicaciones , Caries Dental/epidemiología , Susceptibilidad a Caries Dentarias , Femenino , Reflujo Gastroesofágico/complicaciones , Reflujo Gastroesofágico/epidemiología , Humanos , Masculino , Mucosa Bucal , Adulto Joven
5.
Medicine (Baltimore) ; 100(9): e24934, 2021 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-33655959

RESUMEN

ABSTRACT: Oral cancer is one of the leading causes of cancer death, which are mostly preceded by oral potentially malignant disorders (OPMDs). Taiwanese government launched a free oral cancer screening program. The aim of this study was to analyze the malignant transformation rate of OPMDs.This study was based on national-wide oral screening databases. 3,362,232 people were enrolled. Patients clinically diagnosed with leukoplakia, erythroplakia, oral submucosal fibrosis (OSF), oral verrucous hyperplasia (OVH), and oral lichen planus (OLP), from 2010 to 2013, were identified. We followed up OPMD patients in cancer registry databases to analyze the malignant transformation rate.The malignant transformation rates from the highest to the lowest were: OVH > OSF > erythroplakia > OLP > leukoplakia. The malignant transformation rate was 24.55, 12.76, 9.75, 4.23, and 0.60 per 1000 person-years in the OVH, OSF, erythroplakia, leukoplakia, and comparison cohort. The hazard ratio was 8.19 times higher in the OPMD group compared with comparison cohort group, after age and habit adjustment. Female patients with OPMDs had a high risk of malignant transformation.Nationwide screening is very important for early diagnosis. OVH had the highest malignant transformation possibility. Female OPMD patients are a rare but have a relatively high malignant transformation rate.


Asunto(s)
Transformación Celular Neoplásica/patología , Detección Precoz del Cáncer/métodos , Mucosa Bucal/patología , Neoplasias de la Boca/epidemiología , Vigilancia de la Población/métodos , Lesiones Precancerosas , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Morbilidad/tendencias , Neoplasias de la Boca/patología , Taiwán/epidemiología
6.
Nat Commun ; 12(1): 1689, 2021 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-33727548

RESUMEN

Administration of drugs via the buccal route has attracted much attention in recent years. However, developing systems with satisfactory adhesion under wet conditions and adequate drug bioavailability still remains a challenge. Here, we propose a mussel-inspired mucoadhesive film. Ex vivo models show that this film can achieve strong adhesion to wet buccal tissues (up to 38.72 ± 10.94 kPa). We also demonstrate that the adhesion mechanism of this film relies on both physical association and covalent bonding between the film and mucus. Additionally, the film with incorporated polydopamine nanoparticles shows superior advantages for transport across the mucosal barrier, with improved drug bioavailability (~3.5-fold greater than observed with oral delivery) and therapeutic efficacy in oral mucositis models (~6.0-fold improvement in wound closure at day 5 compared with that observed with no treatment). We anticipate that this platform might aid the development of tissue adhesives and inspire the design of nanoparticle-based buccal delivery systems.


Asunto(s)
Biomimética , Bivalvos/química , Sistemas de Liberación de Medicamentos , Mucosa Bucal/fisiología , Adhesividad , Administración Bucal , Animales , Línea Celular , Dexametasona/farmacología , Dihidroxifenilalanina/química , Liberación de Fármacos , Humanos , Indoles/toxicidad , Masculino , Mucinas/química , Moco/química , Nanopartículas/química , Nanopartículas/ultraestructura , Tamaño de la Partícula , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido Poliglicólico/toxicidad , Polímeros/toxicidad , Alcohol Polivinílico/química , Alcohol Polivinílico/toxicidad , Ratas Sprague-Dawley , Espectrofotometría Ultravioleta , Porcinos , Distribución Tisular
7.
Medicine (Baltimore) ; 100(10): e24742, 2021 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-33725829

RESUMEN

ABSTRACT: Oral microbiota has been implicated in pathogenesis of recurrent aphthous stomatitis (RAS), which is a common mucosal disorder with unclear etiology. This study has explored the association between oral microbiota disorder and RAS in high-risk young female population.Forty-five young females were enrolled, including 24 RAS patients and 21 healthy individuals. Oral microbiome was analyzed by Illumina Miseq sequencing.Oral microbiota associated with RAS was characterized by the lower alpha-diversity indices (Chao1 and ACE). Several infectious pathogens increased in RAS, such as genera Actinobacillus, Haemophilus, Prevotella and Vibrio. The PICRUSt analysis indicated that the oral microbiota might be related with the up-regulation of genes involving infectious and neurodegenerative diseases, environmental adaptation, the down-regulation of genes involving basal metabolism, such as carbohydrate, energy, and amino acid metabolism.This study indicated that oral microbiota may play a significant role in RAS development.


Asunto(s)
Microbiota , Mucosa Bucal/microbiología , Estomatitis Aftosa/microbiología , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Recurrencia , Saliva/microbiología , Adulto Joven
8.
Ned Tijdschr Tandheelkd ; 128(3): 145-149, 2021 Mar.
Artículo en Holandés | MEDLINE | ID: mdl-33734219

RESUMEN

A 48 year old woman was referred by her general practitioner to an oral and maxillofacial surgeon because of an asymptomatic, slow growing intra-oral tumor since three years. There were no sensory and motor symptoms. A well-defined tumor of 5 cm in diameter was located in the right cheek between the zygomaticus arch and the labial commissure. The skin and the intra-oral mucosa were intact without any change in colour or texture. The MRI showed a solitary mass with benign characteristics. A transoral radical excisional biopsy was performed under general anesthesia. Histopathological examination revealed a rare soft tissue perineurioma tumor.


Asunto(s)
Neoplasias de la Vaina del Nervio , Neoplasias de los Tejidos Blandos , Biopsia , Mejilla , Femenino , Humanos , Persona de Mediana Edad , Mucosa Bucal , Neoplasias de la Vaina del Nervio/diagnóstico , Neoplasias de la Vaina del Nervio/cirugía
9.
Klin Lab Diagn ; 66(2): 99-103, 2021 Mar 13.
Artículo en Inglés | MEDLINE | ID: mdl-33734643

RESUMEN

At the present stage of development of society, the issues of preserving and strengthening the most important productive force that determine the economic development and national security of the country remain relevant. Metallurgy is one of the basic industries in Russia, which forms up to 20% of GDP. This study assesses the condition of the oral mucosa in workers in the industry. To evaluate the formation of micronuclei in buccal cells as an early biomarker of health disorders as a result of occupational exposure to production factors of a metallurgical plant. Hygienic and clinical laboratory tests were carried out for workers of the metallurgical plant of the Republic of Bashkortostan. Cytological studies of the buccal epithelium were performed. Statistical processing of the results was carried out using the applied programs IBM, SPSS, Statistics, Microsoft Excel. The general assessment of working conditions in accordance with the criteria of R.2.2.2006-05 for workers of the metallurgical plant was established as 3.2-3.3. Analysis of the buccal epithelium revealed the occurrence of cells with cytogenetic disorders in the workers of the main group. Cells with atypical nuclei were identified in workers with a duration of contact with unfavorable factors of production for more than ten years. Signs of nuclear destruction were revealed, characterizing an increase in apoptotic activity in workers with prolonged contact times. Studies have shown that with more than 10 years of work experience, proliferation processes prevail over differentiation processes. The results obtained can be used as diagnostic methods that expand the prospects for identifying pre-pathological and pathological conditions.


Asunto(s)
Mucosa Bucal , Exposición Profesional , Baskiria , Análisis Citogenético , Epitelio , Humanos , Metalurgia , Exposición Profesional/efectos adversos , Federación de Rusia
10.
J Med Case Rep ; 15(1): 97, 2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33632292

RESUMEN

BACKGROUND: Lichen planus is a rare autoimmune disease primarily affecting the skin and mucous membranes of the oral mucosa, vulva, and vagina. Diagnosis is difficult and often delayed as the clinicians do not associate the oral symptoms with the genital symptoms. This has a negative impact on the out-of-pocket expenditure and quality of life of the patients. We report this case, as only anecdotal cases have been reported so far from a developing country such as India. We highlight the unindicated hysterectomy that the patient had undergone because of lack of awareness regarding this condition. Our case report also highlights the importance of the multidisciplinary team approach to optimize outcomes and avoid unnecessary morbidity to such patients. CASE PRESENTATION: We report a North-Indian patient with oro-vaginal-vulvar lichen planus who presented to us with complaints of recurrent vulvovaginal symptoms for the last 5 years. She had been previously treated with multiple courses of antibiotics, antifungals, and topical steroids over the course of 3 years and finally offered laparoscopic-assisted vaginal hysterectomy (LAVH) by a private practitioner but got no relief. She also had complained of oral symptoms in the form of a burning sensation after eating spicy food, but did not seek any treatment for this. After multidisciplinary team discussion, a final diagnosis of oro-vaginal-vulvar lichen planus was made at our institute based on the clinical and histopathological findings. The patient was immediately started on oral prednisolone to which she responded with improvement in her symptoms. CONCLUSION: Lichen planus is a chronic painful condition with significant impact on the quality of life. Women often suffer for several years before an accurate diagnosis is made. Treatment is challenging and needs to be individualized with a multidisciplinary approach to prevent progressive anatomical distortion and associated morbidity.


Asunto(s)
Histerectomía Vaginal , Liquen Plano/diagnóstico , Liquen Plano/tratamiento farmacológico , Liquen Plano/cirugía , Prednisolona/uso terapéutico , Procedimientos Innecesarios , Adulto , Femenino , Humanos , India/epidemiología , Mucosa Bucal/patología , Calidad de Vida , Resultado del Tratamiento , Enfermedades Vaginales/diagnóstico , Enfermedades de la Vulva/diagnóstico
11.
Medicine (Baltimore) ; 100(3): e23609, 2021 Jan 22.
Artículo en Inglés | MEDLINE | ID: mdl-33545933

RESUMEN

RATIONALE: Traditional free gingival graft (FGG) technique is usually used for patients with insufficient peri-implant keratinized mucosa. However, this technique often requires a second surgical area which increases the pain as well as the risk of infection in patients. Xenogeneic collagen matrix (XCM) membrane technique can obtain good results for keratinized mucosa increment. PATIENT CONCERNS: The patient was a 66-year-old healthy female with loss of left mandibular first molar and second molar (FDI #36, #37) for 5 years. Two implants were placed submucosally for 3 months with no interference, while a stage II surgery was needed. DIAGNOSIS: Probing depth measurements suggested that the mesial, medial, and distal widths of buccal keratinized mucosa within the edentulous area were 0.5, 0.5, and 1 mm, respectively, which were insufficient to maintain the health of peri-implant tissues. INTERVENTIONS: Keratinized mucosa augmentation guided by XCM membranes was performed to increase the inadequate buccal keratinized mucosa. OUTCOMES: After 2 months of healing, the widths of mesial, medial, and distal buccal keratinized mucosa were 4, 3, and 3 mm, respectively, and the thickness of the augmented mucosa was 4 mm. Then a stage II surgery was followed. The patient was satisfied with the outcomes of keratinized mucosa augmentation. LESSONS: Keratinized mucosa augmentation guided by double XCM membrane technique can be applied to cases with keratinized mucosa width within 2 mm around implants.


Asunto(s)
Colágeno/administración & dosificación , Implantes Dentales , Mandíbula/cirugía , Mucosa Bucal/trasplante , Anciano , Femenino , Humanos , Cicatrización de Heridas
12.
Int Dent J ; 71(1): 9-20, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33616057

RESUMEN

INTRODUCTION: Graft-versus-host disease (GVHD) is a complication of haematopoietic stem cell transplantation (HSCT). GVHD may also develop following solid transplants or blood transfusions if white blood cells are transferred. GVHD affects multiple organs, including the oral tissues. OBJECTIVE: This pictorial review provides a background of GVHD to dental practitioners, describes the most common oral manifestations of GVHD and highlights the main treatment modifications needed to deliver dental care to patients with GVHD. METHODS: A narrative review enhanced with clinical photographs. RESULTS: Acute GVHD may manifest in the oral mucosa; however, it often develops immediately following HSCT when routine dental treatment is postponed. Chronic GVHD may manifest in the oral mucosa, the salivary glands and the musculoskeletal compartment. It may indirectly affect the teeth and the oral flora, putting the patient at risk for infections. Importantly, GVHD poses an increased risk for oral cancer. CONCLUSION: GVHD has a wide range of oral manifestations, some of which may affect dental treatment.


Asunto(s)
Enfermedad Injerto contra Huésped , Trasplante de Células Madre Hematopoyéticas , Enfermedad Crónica , Odontólogos , Enfermedad Injerto contra Huésped/etiología , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Humanos , Mucosa Bucal , Rol Profesional
13.
Neurosci Lett ; 748: 135694, 2021 03 23.
Artículo en Inglés | MEDLINE | ID: mdl-33600902

RESUMEN

Patients with COVID-19 often complain of smell and taste disorders (STD). STD emerge early in the course of the disease, seem to be more common in SARS-CoV-2 infection than in other upper respiratory tract infections, and could in some cases persist for long after resolution of respiratory symptoms. Current evidence suggests that STD probably result from a loss of function of olfactory sensory neurons and taste buds, mainly caused by infection, inflammation, and subsequent dysfunction of supporting non-neuronal cells in the mucosa. However, the possible occurrence of other mechanisms leading to chemosensory dysfunction has also been hypothesized, and contrasting data have been reported regarding the direct infection of sensory neurons by SARS-CoV-2. In this mini-review, we summarize the currently available literature on pathogenesis, clinical manifestations, diagnosis, and outcomes of STD in COVID-19 and discuss possible future directions of research on this topic.


Asunto(s)
/complicaciones , Trastornos del Olfato/etiología , Trastornos del Gusto/etiología , /inmunología , Humanos , Mucosa Bucal/inmunología , Mucosa Bucal/patología , Trastornos del Olfato/diagnóstico , Trastornos del Olfato/epidemiología , Trastornos del Olfato/fisiopatología , Mucosa Olfatoria/inmunología , Mucosa Olfatoria/patología , Neuronas Receptoras Olfatorias/inmunología , Neuronas Receptoras Olfatorias/patología , Olfato/fisiología , Gusto/fisiología , Papilas Gustativas/inmunología , Papilas Gustativas/patología , Trastornos del Gusto/diagnóstico , Trastornos del Gusto/epidemiología , Trastornos del Gusto/fisiopatología
14.
Cell Host Microbe ; 29(2): 148-149, 2021 02 10.
Artículo en Inglés | MEDLINE | ID: mdl-33571439

RESUMEN

Much of our understanding of the way mucosal surfaces achieve a harmonious balance with their resident commensal microbiota derives from analysis of this interplay in the gut. Koren et al. (2021) interrogate the dynamics of this relationship in the mouth during early life and find that highly tissue-specific responses facilitate maturation.


Asunto(s)
Inmunidad Mucosa , Microbiota , Epitelio , Boca , Mucosa Bucal
15.
Ned Tijdschr Tandheelkd ; 128(2): 103-111, 2021 Feb.
Artículo en Holandés | MEDLINE | ID: mdl-33605260

RESUMEN

Oral leukoplakia is the most common potentially malignant disorder of the oral mucosa with a rate of malignant transformation into oral squamous cell carcinoma of 1-2% annually. The presence or absence of dysplasia as defined by the WHO is an important histological marker for malignant transformation risk assessment, but is not sufficiently accurate for patient stratification. We investigated whether identifying differentiated dysplasia contributes to oral leukoplakia malignant transformation risk assessment. We investigated whether classic or differentiated dysplasia were present in 84 oral leukoplakias. In 25 of these patients a squamous cell carcinoma developed during follow-up. Risk of malignant progression of oral leukoplakia increased from 3.3 (HR, p = 0.002) when only classic dysplasia was considered to 7.4 (HR, p = 0.001) when both classic and differentiated dysplasia were combined. This study demonstrates that identifying differentiated dysplasia as a separate type of dysplasia is important for the prognosis and stratification of patients with oral leukoplakia.


Asunto(s)
Carcinoma de Células Escamosas , Neoplasias de la Boca , Lesiones Precancerosas , Carcinoma de Células Escamosas/diagnóstico , Transformación Celular Neoplásica , Humanos , Leucoplasia Bucal/diagnóstico , Mucosa Bucal , Neoplasias de la Boca/diagnóstico , Lesiones Precancerosas/diagnóstico
16.
Medicine (Baltimore) ; 100(6): e23626, 2021 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-33578511

RESUMEN

BACKGROUND: The number of adult patients affected by the human immunodeficiency virus (HIV) still remains high, mainly in the developing countries. However, only a few affected patients fail to experience oral lesions in the course of their experience with the virus. In particular, oral mucosa ulcers detected among HIV patients may be severe, which depictions may inhibit oral functioning and change patients' quality of life. Thus, it can result in considerable morbidity among this group of patients. To this end, the present study aims to examine the topical agent's clinical therapeutic efficacy among adult patients suffering from HIV-related oral mucosa ulcers. METHODS: For the investigation, only randomized controlled trials on any topical agent used to treat adult patients with HIV oral mucosa ulcers are to be explored from different databases: PubMed, the Cochrane Library, PsycINFO, EMBASE, SCOPUS, Web of Science, China Biomedical Literature Database, China National Knowledge Infrastructure, VIP, and WanFang databases. All databases will be searched from their inceptions to October 2020. Additionally, 2 independent authors will evaluate the possibly eligible studies to be included in the study. They will also perform data's trial extraction and risk of bias assessment. Accordingly, all data will be analysed by means of the RevMan 5.3 software. RESULTS: The present study seeks to evaluate the topical agents' clinical therapeutic efficacy to treat adult patients with HIV-related oral mucosa ulcers. CONCLUSION: The study can be applicable in providing evidence of any topical agents for treating adult patients with HIV-related oral mucosa ulcers for clinical practice. PROTOCOL REGISTRATION NUMBER: DOI 10.17605/OSF.IO/5CYR2 (https://osf.io/5cyr2/).


Asunto(s)
Antiinfecciosos Locales/uso terapéutico , Infecciones por VIH/complicaciones , Mucosa Bucal/patología , Úlceras Bucales/tratamiento farmacológico , Adulto , Antiinfecciosos Locales/administración & dosificación , Estudios de Casos y Controles , VIH/efectos de los fármacos , Infecciones por VIH/epidemiología , Humanos , Mucosa Bucal/virología , Úlceras Bucales/psicología , Calidad de Vida , Ensayos Clínicos Controlados Aleatorios como Asunto , Resultado del Tratamiento
17.
Science ; 371(6526)2021 01 15.
Artículo en Inglés | MEDLINE | ID: mdl-33446526

RESUMEN

Human monogenic disorders have revealed the critical contribution of type 17 responses in mucosal fungal surveillance. We unexpectedly found that in certain settings, enhanced type 1 immunity rather than defective type 17 responses can promote mucosal fungal infection susceptibility. Notably, in mice and humans with AIRE deficiency, an autoimmune disease characterized by selective susceptibility to mucosal but not systemic fungal infection, mucosal type 17 responses are intact while type 1 responses are exacerbated. These responses promote aberrant interferon-γ (IFN-γ)- and signal transducer and activator of transcription 1 (STAT1)-dependent epithelial barrier defects as well as mucosal fungal infection susceptibility. Concordantly, genetic and pharmacologic inhibition of IFN-γ or Janus kinase (JAK)-STAT signaling ameliorates mucosal fungal disease. Thus, we identify aberrant T cell-dependent, type 1 mucosal inflammation as a critical tissue-specific pathogenic mechanism that promotes mucosal fungal infection susceptibility in mice and humans.


Asunto(s)
Candida albicans/inmunología , Candidiasis Mucocutánea Crónica/genética , Candidiasis Mucocutánea Crónica/inmunología , Inmunidad Mucosa/inmunología , Poliendocrinopatías Autoinmunes/genética , Poliendocrinopatías Autoinmunes/inmunología , Adolescente , Adulto , Anciano , Animales , Modelos Animales de Enfermedad , Femenino , Humanos , Inmunidad Mucosa/genética , Vigilancia Inmunológica/genética , Vigilancia Inmunológica/inmunología , Interferón gamma/genética , Interleucinas/genética , Quinasas Janus/genética , Masculino , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Mucosa Bucal/inmunología , Mucosa Bucal/patología , Receptores de Interleucina-17/genética , Factor de Transcripción STAT1/genética , Linfocitos T/inmunología , Adulto Joven
18.
BMJ Case Rep ; 14(1)2021 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-33509889

RESUMEN

Electronic cigarettes (also known as e-cigarettes or electronic nicotine delivery systems) were invented in China in 2003 then introduced to the British market in 2007. They remain popular among the public and are deemed to be effective in reducing tobacco smoking (the UK being one of the first countries to embrace them in a harm reduction policy). However, reports in the media of e-cigarettes exploding are of concern, considering the potential functional and psychological impairment that lifelong disfigurement will cause, especially given their uptake among people of any age. We present a case of this rare, but dramatic, effect of e-cigarette use as a warning to the public.


Asunto(s)
Quemaduras/etiología , Sistemas Electrónicos de Liberación de Nicotina , Explosiones , Fracturas Conminutas/etiología , Fracturas Maxilares/etiología , Fracturas de los Dientes/etiología , Fracturas Conminutas/diagnóstico por imagen , Humanos , Imagenología Tridimensional , Masculino , Fracturas Maxilares/diagnóstico por imagen , Traumatismos Maxilofaciales/diagnóstico por imagen , Traumatismos Maxilofaciales/etiología , Mucosa Bucal/lesiones , Tomografía Computarizada por Rayos X , Fracturas de los Dientes/diagnóstico por imagen , Traumatismos de los Dientes/diagnóstico por imagen , Traumatismos de los Dientes/etiología , Raíz del Diente/lesiones , Adulto Joven
19.
Methods Mol Biol ; 2260: 133-143, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33405035

RESUMEN

Microbial interactions with epithelial barriers are important steps preceding disease. Infections with Candida albicans are no exception. This opportunistic fungus, commonly harmlessly residing in close proximity to human epithelia, can shift to a more pathogenic form, can invade tissues, and cause disease. Pathogenesis, in C. albicans as well as in many other microorganisms, is characterized by three important steps: adhesion to-, invasion into-, and damage of host cells. In this book chapter, we describe three well-established protocols that allow us to differentially stain C. albicans cells adhering to and invading into host cells, therefore allowing quantifications of such processes. We also describe a common host cell cytotoxicity assay that employs a commercial kit, adapted to C. albicans.


Asunto(s)
Candida albicans/patogenicidad , Candidiasis Bucal/microbiología , Adhesión Celular , Células Epiteliales/microbiología , Microscopía Fluorescente , Mucosa Bucal/microbiología , Candidiasis Bucal/metabolismo , Candidiasis Bucal/patología , Línea Celular , Supervivencia Celular , Células Epiteliales/metabolismo , Células Epiteliales/patología , Interacciones Huésped-Patógeno , Humanos , Mucosa Bucal/metabolismo , Mucosa Bucal/patología
20.
In Vitro Cell Dev Biol Anim ; 57(2): 148-159, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33447968

RESUMEN

Three-dimensional, organotypic models of the oral mucosa have been developed to study a wide variety of phenomena occurring in the oral cavity. Although a number of models have been developed in academic research labs, only a few models have been commercialized. Models from academic groups offer a broader range of phenotypes while the commercial models are more focused on the oral and gingival mucosa. The commercialized models are manufactured under highly controlled conditions and meet the requirements of quality standards, which leads to high levels of reproducibility. These in vitro models have been used to evaluate the irritancy of oral care products such as toothpastes, mouthwashes, and mucoadhesives. The effects of cigarette smoke on oral cavity tissues have been studied and compared to those of e-cigarettes. Oral tissue models have facilitated investigation of the mechanisms of oral mucositis and oral candidiasis and have been used to examine transbuccal drug delivery rates and the absorption of nanoparticles. Infection studies have investigated the effects of HIV-1 along with the effects of commensal and pathogenic bacteria. More recently, a differentiated oral tissue model has been shown to express the ACE2 receptor, which is known to be important for the receptor-mediated entry of the SARS-CoV-2 coronavirus into human cells and tissues. Hence, oral mucosal models may find application in determining whether viral infection of the oral mucosa is possible and whether such infection has implications vis-a-vis the current COVID-19 pandemic. As is apparent, these models are used in a broad variety of applications and often offer advantages versus animal models in terms of reproducibility, avoiding species extrapolation, and the ethical concerns related to human and animal experimentation. The goals of this paper are to review commercially available models of the human buccal and gingival mucosa and highlight their use to gain a better understanding of a broad range of phenomena affecting tissues in the oral cavity.


Asunto(s)
Materiales Dentales/efectos adversos , Infecciones , Mucosa Bucal/citología , Mucosa Bucal/virología , Técnicas de Cultivo de Tejidos/métodos , /transmisión , Sistemas de Liberación de Medicamentos/efectos adversos , Sistemas de Liberación de Medicamentos/métodos , Humanos , Infecciones/microbiología , Infecciones/virología , Boca , Mucosa Bucal/microbiología , Mucosa Bucal/patología , Control de Calidad , Técnicas de Cultivo de Tejidos/instrumentación , Ingeniería de Tejidos , Tabaco , Pastas de Dientes/efectos adversos , Rayos Ultravioleta/efectos adversos
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