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1.
Int J Nanomedicine ; 16: 1993-2011, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33727811

RESUMEN

Background: Even with considerable improvement in treatment of epithelial ovarian cancer achieved in recent years, an increasing chemotherapy resistance and disease 5-year relapse is recorded for a majority part of patients that encourages the search for better therapeutic options. Gold nanoparticles (Au NPs) due to plethora of unique physiochemical features are thoroughly tested as drug delivery, radiosensitizers, as well as photothermal and photodynamic therapy agents. Importantly, due to highly controlled synthesis, it is possible to obtain nanomaterials with directed size and shape. Methods: In this work, we developed novel elongated-type gold nanoparticles in the shape of nanopeanuts (AuP NPs) and investigated their cytotoxic potential against ovarian cancer cells SKOV-3 using colorimetric and fluorimetric methods, Western blot, flow cytometry, and fluorescence microscopy. Results: Peanut-shaped gold nanoparticles showed high anti-cancer activity in vitro against SKOV-3 cells at doses of 1-5 ng/mL upon 72 hours treatment. We demonstrate that AuP NPs decrease the viability and proliferation capability of ovarian cancer cells by triggering cell apoptosis and autophagy, as evidenced by flow cytometry and Western blot analyses. The overproduction of reactive oxygen species (ROS) was noted to be a critical mediator of AuP NPs-mediated cell death. Conclusion: These data indicate that gold nanopeanuts might be developed as nanotherapeutics against ovarian cancer.


Asunto(s)
Apoptosis , Autofagia , Oro/química , Nanopartículas del Metal/química , Neoplasias Ováricas/patología , Especies Reactivas de Oxígeno/metabolismo , Apoptosis/efectos de los fármacos , Proteínas Reguladoras de la Apoptosis/metabolismo , Arachis , Autofagia/efectos de los fármacos , Carcinoma Epitelial de Ovario/tratamiento farmacológico , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Femenino , Humanos , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Nanopartículas del Metal/ultraestructura , Neoplasias Ováricas/tratamiento farmacológico , Oxidación-Reducción
2.
J Vis Exp ; (168)2021 02 09.
Artículo en Inglés | MEDLINE | ID: mdl-33645589

RESUMEN

Kidney stones are becoming more prevalent worldwide in adults and children. The most common type of kidney stone is comprised of calcium oxalate (CaOx) crystals. Crystalluria occurs when urine becomes supersaturated with minerals (e.g., calcium, oxalate, phosphate) and precedes kidney stone formation. Standard methods to assess crystalluria in stone formers include microscopy, filtration, and centrifugation. However, these methods primarily detect microcrystals and not nanocrystals. Nanocrystals have been suggested to be more harmful to kidney epithelial cells than microcrystals in vitro. Here, we describe the ability of Nanoparticle Tracking analysis (NTA) to detect human urinary nanocrystals. Healthy adults were fed a controlled oxalate diet prior to drinking an oxalate load to stimulate urinary nanocrystals. Urine was collected for 24 hours before and after the oxalate load. Samples were processed and washed with ethanol to purify samples. Urinary nanocrystals were stained with the calcium binding fluorophore, Fluo-4 AM. After staining, the size and count of nanocrystals were determined using NTA. The findings from this study show NTA can efficiently detect nanocrystalluria in healthy adults. These findings suggest NTA could be a valuable early detection method of nanocrystalluria in patients with kidney stone disease.


Asunto(s)
Compuestos de Anilina/química , Oxalato de Calcio/orina , Oro/química , Nanopartículas del Metal/química , Nanopartículas/química , Xantenos/química , Adulto , Humanos
3.
Anal Chim Acta ; 1154: 338330, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33736792

RESUMEN

The recent outbreak of coronavirus disease 2019 (COVID-19) is highly infectious, which threatens human health and has received increasing attention. So far, there is no specific drug or vaccine for COVID-19. Therefore, it is urgent to establish a rapid and sensitive early diagnosis platform, which is of great significance for physical separation of infected persons after rapid diagnosis. Here, we propose a colorimetric/SERS/fluorescence triple-mode biosensor based on AuNPs for the fast selective detection of viral RNA in 40 min. AuNPs with average size of 17 nm were synthesized, and colorimetric, surface enhanced Raman scattering (SERS), and fluorescence signals of sensors are simultaneously detected based on their basic aggregation property and affinity energy to different bio-molecules. The sensor achieves a limit detection of femtomole level in all triple modes, which is 160 fM in absorbance mode, 259 fM in fluorescence mode, and 395 fM in SERS mode. The triple-mode signals of the sensor are verified with each other to make the experimental results more accurate, and the capacity to recognize single-base mismatch in each working mode minimizes the false negative/positive reading of SARS-CoV-2. The proposed sensing platform provides a new way for the fast, sensitive, and selective detection of COVID-19 and other diseases.


Asunto(s)
Técnicas Biosensibles/métodos , Nanopartículas del Metal/química , ARN Viral/análisis , /genética , /virología , Oro/química , Humanos , Límite de Detección , Tamaño de la Partícula , ARN Viral/química , Espectrometría Raman , Glicoproteína de la Espiga del Coronavirus/genética
4.
Molecules ; 26(4)2021 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-33672102

RESUMEN

Macroalgae are sources of bioactive compounds that are interesting from both a chemical and a medical point of view. Although their use in biomedicine has increased significantly in recent years, tests conducted to date have been mostly related to species from temperate latitudes, with the potential application of Antarctic biodiversity being minor. The wide variety of algae species present on Antarctic coastal areas can be a source of new antioxidants. Bearing this in mind, the brown macroalgae Desmarestia antarctica (DA) and the red Iridaea cordata (IC) were selected for the preparation of aqueous extracts with the aim of analyzing their antioxidant activity. This analysis was performed by determining reducing power, total phenolic content, and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity. Furthermore, both extracts were employed to synthesize gold and silver nanoparticles. The nanomaterials were fully characterized by means of UV-Visible spectroscopy, transmission electron microscopy, Z potential measurements, and Fourier transform infrared spectroscopy, which confirmed the formation of stable, spherical nanoparticles with mean diameters of 13.7 ± 3.1 and 17.5 ± 3.7 nm for Ag@DA and Ag@IC and 12.6 ± 1.9 and 12.3 ± 1.6 nm for Au@DA and Au@IC. Antioxidant assays were performed after the synthesis of the nanomaterials to evaluate their possible synergistic effect with the extracts. The results suggest that polysaccharides and proteins may play a key role in the process of reduction and stabilization. Finally, for the sake of comparison, the results obtained for the Antarctic macroalgae Desmarestia menziesii and Palmaria decipiens have also been considered in the present work.


Asunto(s)
Antioxidantes/farmacología , Oro/metabolismo , Nanopartículas del Metal/química , Phaeophyta/química , Rhodophyta/química , Plata/metabolismo , Regiones Antárticas , Antioxidantes/síntesis química , Antioxidantes/química , Compuestos de Bifenilo/antagonistas & inhibidores , Oro/química , Tamaño de la Partícula , Picratos/antagonistas & inhibidores , Plata/química
5.
Food Chem ; 352: 129354, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33677209

RESUMEN

Biosensors have been widely applied in tests for allergens, but on-site detection remains a challenge. Herein, we proposed a detection procedure for peanut Ara h 1 as a representative allergen, which was extracted from a cookie, thereby minimising the need for any complex pretreatment that was difficult to perform, and enabling the visual detection of the target without the use of analytical equipment. The extraction procedure was performed in less than 30 min using a syringe and filter (0.45 µm). The detection method for Ara h 1 was based on the aggregation of switchable linkers (SL) and gold nanoparticles (AuNP), and the presence of 0.19 mg peanut protein per 30 g of cookie could be confirmed within 30 min based on the AuNP/SL concentration ratio by the precipitation. This proposed procedure could be successfully applied to the detection of a wide range of food allergens.


Asunto(s)
Antígenos de Plantas/análisis , Antígenos de Plantas/aislamiento & purificación , Precipitación Química , Oro/química , Proteínas de la Membrana/análisis , Proteínas de la Membrana/aislamiento & purificación , Nanopartículas del Metal/química , Proteínas de Plantas/análisis , Proteínas de Plantas/aislamiento & purificación , Antígenos de Plantas/inmunología , Humanos , Proteínas de la Membrana/inmunología , Hipersensibilidad al Cacahuete , Proteínas de Plantas/inmunología
6.
ACS Sens ; 6(3): 1086-1093, 2021 03 26.
Artículo en Inglés | MEDLINE | ID: mdl-33683104

RESUMEN

The outbreak of COVID-19 caused a worldwide public health crisis. Large-scale population screening is an effective means to control the spread of COVID-19. Reverse transcription-polymerase chain reaction (RT-qPCR) and serology assays are the most available techniques for SARS-CoV-2 detection; however, they suffer from either less sensitivity and accuracy or low instrument accessibility for screening. To balance the sensitivity, specificity, and test availability, here, we developed enhanced colorimetry, which is termed as a magnetic pull-down-assisted colorimetric method based on the CRISPR/Cas12a system (M-CDC), for SARS-CoV-2 detection. By this method, SARS-CoV-2 RNA from synthetic sequences and cultured viruses can be detected by the naked eye based on gold nanoparticle (AuNP) probes, with a detection limit of 50 RNA copies per reaction. With CRISPR/Cas12a-assisted detection, SARS-CoV-2 can be specifically distinguished from other closely related viruses. M-CDC was further used to analyze 41 clinical samples, whose performance was 95.12%, consistent with that of an approved Clinical RT-qPCR Diagnosis kit. The developed M-CDC method is not dependent on sophisticated instruments, which makes it potentially valuable to be applied for SARS-CoV-2 screening under poor conditions.


Asunto(s)
/métodos , ARN Viral/análisis , /genética , Proteínas Bacterianas , Proteínas Asociadas a CRISPR , Sistemas CRISPR-Cas , Línea Celular Tumoral , Colorimetría , ADN/química , Sondas de ADN , Endodesoxirribonucleasas , Oro/química , Humanos , Nanopartículas del Metal/química
7.
Food Chem ; 352: 129330, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33657486

RESUMEN

Pregnancy test strips are one of the most mature and widely used commercial lateral flow devices used to determine pregnancy. Being a simple and rapid detection method, human chorionic gonadotropin (hCG) was used with different aptamers (hCG-apt) as probes for the detection of metal ions, small organic molecules, and proteins. Quantitative detection of target analytes was achieved using a smartphone app and a portable device developed in our laboratory. The results showed detection ranges of 1 nM-1 µM, 0.1 nM-10 µM and 32 nM-500 nM for Pb2+, chloramphenicol, and ß-lactoglobulin, respectively, and the corresponding visual detection limits in dairy products were 5 nM, 1 nM and 50 nM, respectively. Based on these results, rapid detection of multiple analytes can be realized through aptamer modification, thereby broadening the application range of commercial lateral flow devices for analysis of food chemistry.


Asunto(s)
Aptámeros de Nucleótidos/metabolismo , Productos Lácteos/análisis , Compuestos Férricos/química , Análisis de los Alimentos/instrumentación , Grafito/química , Pruebas de Embarazo/instrumentación , Teléfono Inteligente , Animales , Femenino , Oro/química , Humanos , Límite de Detección , Embarazo
8.
Molecules ; 26(5)2021 Feb 25.
Artículo en Inglés | MEDLINE | ID: mdl-33668968

RESUMEN

The unique chemical, optical, and electrical characteristics of nanoparticles make their utilization highly successful in every field of biological sciences as compared to their bulk counterpart. These properties arise as a result of their miniature size, which provides them an excellent surface area-to-volume ratio, inner structure, and shape, and hence increases their surface characteristics. Therefore, this study was undertaken to engineer gold nanoparticles (AuNPs) for improving their catalytic activity and stability in biotechnological processes. The characterization of AuNPs was performed by XRD, UV spectra, and TEM. The synthesized AuNPs were surface-modified by polyvinyl alcohol (PVA) for binding the enzyme in excellent yield. The developed immobilized enzyme system (PVA-AuNPs-ß-galactosidase) displayed pH optima at pH 7.0 and temperature optima at 40 °C. Moreover, the stability of PVA-AuNPs-ß-galactosidase was significantly enhanced at wider pH and temperature ranges and at higher galactose concentrations, in contrast to the free enzyme. ß-galactosidase bound to PVA-modified AuNPs exhibited greater operational activity, even after its sixth reuse. The developed nanosystem may prove useful in producing lactose-free dairy products for lactose-intolerant patients.


Asunto(s)
Productos Lácteos , Oro/química , Lactosa/química , Nanopartículas del Metal/química , beta-Galactosidasa/química , Productos Lácteos/análisis , Estabilidad de Enzimas , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/metabolismo , Oro/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Kluyveromyces/enzimología , Lactosa/metabolismo , Intolerancia a la Lactosa/metabolismo , Prueba de Tolerancia a la Lactosa , Tamaño de la Partícula , Propiedades de Superficie , Temperatura , beta-Galactosidasa/metabolismo
9.
J Vis Exp ; (168)2021 02 17.
Artículo en Inglés | MEDLINE | ID: mdl-33682852

RESUMEN

Temperature control is a recent development that provides an additional degree of freedom to study nanochemistry by liquid cell transmission electron microscopy. In this paper, we describe how to prepare an in situ heating experiment for studying the effect of temperature on the formation of gold nanoparticles driven by radiolysis in water. The protocol of the experiment is fairly simple involving a special liquid cell with uniform heating capabilities up to 100 °C, a liquid-cell TEM holder with flow capabilities and an integrated interface for controlling the temperature. We show that the nucleation and growth mechanisms of gold nanoparticles are drastically impacted by the temperature in liquid cell. Using STEM imaging and nanodiffraction, the evolution of the density, size, shape and atomic structure of the growing nanoparticles are revealed in real time. Automated image processing algorithms are exploited to extract useful quantitative data from video sequences, such as the nucleation and growth rates of nanoparticles. This approach provides new inputs for understanding the complex physico-chemical processes at play during the liquid-phase synthesis of nanomaterials.


Asunto(s)
Nanopartículas del Metal/química , Nanopartículas del Metal/ultraestructura , Microscopía Electrónica de Transmisión , Temperatura , Oro/química , Calefacción , Procesamiento de Imagen Asistido por Computador , Programas Informáticos , Agua/química
10.
Int J Nanomedicine ; 16: 2311-2322, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33776435

RESUMEN

Background: Alzheimer's disease (AD) is a neurodegenerative chronic disorder that causes dementia and problems in thinking, cognitive impairment and behavioral changes. Amyloid-beta (Aß) is a peptide involved in AD progression, and a high level of Aß is highly correlated with severe AD. Identifying and quantifying Aß levels helps in the early treatment of AD and reduces the factors associated with AD. Materials and Methods: This research introduced a dual probe detection system involving aptamers and antibodies to identify Aß. Aptamers and antibodies were attached to the gold (Au) urchin and hybrid on the carbon nanohorn-modified surface. The nanohorn was immobilized on the sensor surface by using an amine linker, and then a Au urchin dual probe was immobilized. Results: This dual probe-modified surface enhanced the current flow during Aß detection compared with the surface with antibody as the probe. This dual probe interacted with higher numbers of Aß peptides and reached the detection limit at 10 fM with R2=0.992. Furthermore, control experiments with nonimmune antibodies, complementary aptamer sequences and control proteins did not display the current responses, indicating the specific detection of Aß. Conclusion: Aß-spiked artificial cerebrospinal fluid showed a similar response to current changes, confirming the selective identification of Aß.


Asunto(s)
Enfermedad de Alzheimer/diagnóstico , Oro/química , Sondas Moleculares/química , Nanopartículas/química , Péptidos beta-Amiloides/metabolismo , Electrodos , Humanos , Límite de Detección , Modelos Lineales , Nanopartículas/ultraestructura , Fragmentos de Péptidos , Multimerización de Proteína , Reproducibilidad de los Resultados , Espectrometría por Rayos X , Propiedades de Superficie
11.
Food Chem ; 352: 129327, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33690077

RESUMEN

This paper describes a voltammetric method and data analysis program developed for the detection of arsenic(III) in commercial apple juice. Arsenic(III) was detected using square wave stripping voltammetry with gold nanoparticle modified screen printed electrodes. The only sample pretreatment performed was the addition of a 100 mM phosphate buffer with a pH of 7. To compensate for interference from high ascorbic acid concentrations, a data analysis program was developed in MATLAB to fit a non-linear baseline, allowing for accurate peak height measurement. With this data analysis program, the developed methodology had a sensitivity of 0.1007 µA (µg L-1)-1 and a limit of detection of 16.73 µg L-1. A comparison between the voltammetric method and graphite furnace atomic absorption spectroscopy showed no bias in the voltammetric results and a good correlation between the two sets of predicted concentrations, with an R2 of 0.939.


Asunto(s)
Arsénico/análisis , Carbono/química , Electroquímica/instrumentación , Jugos de Frutas y Vegetales/análisis , Oro/química , Malus/química , Nanopartículas del Metal/química , Electrodos , Análisis de los Alimentos/instrumentación , Impresión , Factores de Tiempo
12.
Int J Mol Sci ; 22(4)2021 Feb 17.
Artículo en Inglés | MEDLINE | ID: mdl-33671292

RESUMEN

Anticancer drugs, such as fluorouracil (5-FU), oxaliplatin, and doxorubicin (Dox) are commonly used to treat colorectal cancer (CRC); however, owing to their low response rate and adverse effects, the development of efficient drug delivery systems (DDSs) is required. The cellular prion protein PrPC, which is a cell surface glycoprotein, has been demonstrated to be overexpressed in CRC, however, there has been no research on the development of PrPC-targeting DDSs for targeted drug delivery to CRC. In this study, PrPC aptamer (Apt)-conjugated gold nanoparticles (AuNPs) were synthesized for targeted delivery of Dox to CRC. Thiol-terminated PrPC-Apt was conjugated to AuNPs, followed by hybridization of its complementary DNA for drug loading. Finally, Dox was loaded onto the AuNPs to synthesize PrPC-Apt-functionalized doxorubicin-oligomer-AuNPs (PrPC-Apt DOA). The PrPC-Apt DOA were spherical nanoparticles with an average diameter of 20 nm. Treatment of CRC cells with PrPC-Apt DOA induced reactive oxygen species generation by decreasing catalase and superoxide dismutase activities. In addition, treatment with PrPC-Apt DOA inhibited mitochondrial functions by decreasing the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, complex 4 activity, and oxygen consumption rates. Compared to free Dox, PrPC-Apt DOA decreased proliferation and increased apoptosis of CRC cells to a greater degree. In this study, we demonstrated that PrPC-Apt DOA targeting could effectively deliver Dox to CRC cells. PrPC-Apt DOA can be used as a treatment for CRC, and have the potential to replace existing anticancer drugs, such as 5-FU, oxaliplatin, and Dox.


Asunto(s)
Aptámeros de Nucleótidos/química , Neoplasias Colorrectales/tratamiento farmacológico , Doxorrubicina/administración & dosificación , Doxorrubicina/uso terapéutico , Sistemas de Liberación de Medicamentos , Oro/química , Nanopartículas del Metal/química , Proteínas Priónicas/química , Apoptosis/efectos de los fármacos , Catalasa/metabolismo , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Doxorrubicina/farmacología , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Mitocondrias/metabolismo , Coactivador 1-alfa del Receptor Activado por Proliferadores de Peroxisomas gamma/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Esferoides Celulares/efectos de los fármacos , Esferoides Celulares/metabolismo , Superóxido Dismutasa/metabolismo
13.
Int J Nanomedicine ; 16: 2187-2201, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33758506

RESUMEN

The physicochemical and optical properties of silver nanoparticles (SNPs) and gold nanoparticles (GNPs) have allowed them to be employed for various biomedical applications, including delivery, therapy, imaging, and as theranostic agents. However, since they are foreign body systems, they are usually redistributed and accumulated in some vital organs, which can produce toxic effects; therefore, this a crucial issue that should be considered for potential clinical trials. This review aimed to summarize the reports from the past ten years that have used SNPs and GNPs for in vivo studies on the diagnosis and treatment of brain diseases and those related to the central nervous system, emphasizing their toxicity as a crucial topic address. The article focuses on the effect of the nanoparticle´s size and chemical composition as relevant parameters for in vivo toxicity. At the beginning of this review, the general toxicity and distribution studies are discussed separately for SNPs and GNPs. Subsequently, this manuscript analyzes the principal applications of both kinds of nanoparticles for glioma, neurodegenerative, and other brain diseases, and discusses the advances in clinical trials. Finally, we analyze research prospects towards clinical applications for both types of metallic nanoparticles.


Asunto(s)
Enfermedades del Sistema Nervioso Central/patología , Oro/química , Nanopartículas del Metal/química , Nanopartículas del Metal/toxicidad , Tamaño de la Partícula , Plata/química , Pruebas de Toxicidad , Animales , Humanos , Nanopartículas del Metal/ultraestructura , Distribución Tisular/efectos de los fármacos
14.
Int J Nanomedicine ; 16: 2219-2236, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33762822

RESUMEN

Introduction: In this paper, we have designed and formulated, a novel synthesis of doxorubicin (DOX) loaded bimetallic gold nanorods in which gold salt (HAuCl4) is chelated with anthracycline (DOX), diacid polyethylene-glycol (PEG-COOH) and gadolinium salt (GdCl3 * 6 H2O) to form DOX IN-Gd-AuNRs compared with DOX ON-Gd-AuNRs in which the drug was grafted onto the bimetallic pegylated nanoparticle surface by electrostatic adsorption. Material and Method: The physical and chemical evaluation was performed by spectroscopic analytical techniques (Raman spectroscopy, UV-Visible and transmission electron microscopy (TEM)). Magnetic features at 7T were also measured. Photothermal abilities were assessed. Cytotoxicity studies on MIA PaCa-2, human pancreatic carcinoma and TIB-75 hepatocytes cell lines were carried out to evaluate their biocompatibility and showed a 320 fold higher efficiency for DOX after encapsulation. Results: Exhaustive physicochemical characterization studies were conducted showing a mid size of 20 to 40 nm diameters obtained with low polydispersity, efficient synthesis using seed mediated synthesis with chelation reaction with high scale-up, long duration stability, specific doxorubicin release with acidic pH, strong photothermal abilities at 808 nm in the NIR transparency window, strong magnetic r1 relaxivities for positive MRI, well adapted for image guided therapy and therapeutical purpose in biological tissues. Conclusion: In this paper, we have developed a novel theranostic nanoparticle composed of gadolinium complexes to gold ions, with a PEG biopolymer matrix conjugated with antitumoral doxorubicin, providing multifunctional therapeutic features. Particularly, these nano conjugates enhanced the cytotoxicity toward tumoral MIAPaCa-2 cells by a factor of 320 compared to doxorubicin alone. Moreover, MRI T1 features at 7T enables interesting positive contrast for bioimaging and their adapted size for potential passive targeting to tumors by Enhanced Permeability Retention. Given these encouraging antitumoral and imaging properties, this bimetallic theranostic nanomaterial system represents a veritable promise as a therapeutic entity in the field of medicinal applications.


Asunto(s)
Doxorrubicina/uso terapéutico , Gadolinio/química , Oro/química , Nanotubos/química , Nanomedicina Teranóstica , Animales , Antibióticos Antineoplásicos/farmacología , Antibióticos Antineoplásicos/uso terapéutico , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Doxorrubicina/farmacología , Liberación de Fármacos , Endocitosis , Humanos , Concentración 50 Inhibidora , Imagen por Resonancia Magnética , Ratones , Nanotubos/ultraestructura , Neoplasias/tratamiento farmacológico , Espectrofotometría Ultravioleta
15.
Int J Nanomedicine ; 16: 2237-2246, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33762823

RESUMEN

Background: Translation of nanomedical developments into clinical application is receiving an increasing interest. However, its use for oral squamous cell carcinoma (OSCC) diagnosis remains limited. We present an advanced nanophotonic method for oral cancer detection, based on diffusion reflection (DR) measurements of gold-nanorods bio-conjugated to anti-epidermal growth factor receptor (C-GNRs) specifically attached to OSCC cells. Objective: To investigate in a rat model of oral carcinogenesis the targeting potential of C-GNRs to OSCC by using the DR optical method. Materials and Methods: OSCC was induced by the carcinogen 4-nitroquinoline-N-oxide (4NQO). C-GNRs were introduced locally and systemically and DR measurements were recorded from the surface of the rat tongue following illumination with red laser beam. Rats were divided into experimental and control groups. The results were compared with the histologic diagnosis. Results: A total of 75 Wistar-derived rats were enrolled in the study. Local application did not reveal any statistical results. DR measurements following intravenous injection of C-GNRs revealed a significant increase in light absorption in rats with OSCC compare with rats without cancer (p<0.02, sensitivity 100%, specificity 89%). In addition, absorption of light increased significantly in cases of severe dysplasia and cancer (high risk) compared to rats without cancer and rats with mild dysplasia (low risk) (86% sensitivity and 89% specificity, AUC=0.79). Conclusion: Combining nanotechnology and nanophotonics for in vivo diagnosis of OSCC serves as additional tier in the translation of advanced nanomedical developments into clinical applications. The presented method shows a promising potential of nanophotonics for oral cancer identification, and provides support for the use of C-GNRs as a selective drug delivery.


Asunto(s)
Carcinoma de Células Escamosas/diagnóstico , Detección Precoz del Cáncer , Receptores ErbB/antagonistas & inhibidores , Oro/química , Neoplasias de la Boca/diagnóstico , Nanotubos/química , Animales , Carcinoma de Células Escamosas/patología , Difusión , Receptores ErbB/metabolismo , Masculino , Neoplasias de la Boca/tratamiento farmacológico , Ratas Wistar
16.
AAPS PharmSciTech ; 22(2): 64, 2021 Feb 03.
Artículo en Inglés | MEDLINE | ID: mdl-33533992

RESUMEN

The aim of the study was to prepare 5-fluorouracil (5-FU)-loaded biogenic gold nanoparticles with pluronic-based coating (PFGNPs), their optimization (full factorial predicted OBPN-1) and in vitro-ex vivo evaluation. Several formulations were prepared, selected for optimization using Design Expert®, and compared for morphology, 5-FU release kinetics, compatibility, cell line toxicity, in vitro hemocompatibility, and ex vivo intestinal permeation across the rat duodenum, jejunum, and ileum. The pluronic-coated 5-FU-carrying GNPs were spherical, 29.11-178.21 nm in diameter, with a polydispersity index (PDI) range of 0.191-292, and a zeta potential (ZP) range of 11.19-29.21 (-mV). The optimized OBPN-1 (desirability = 0.95) demonstrated optimum size (175.1 nm), %DL as 73.8%, ZP as 21.7 mV, % drug release (DR) as 75.7%, and greater cytotoxicity (viability ~ 8.9%) against the colon cancer cell lines than 5-FU solution (~ 24.91%), and less hemocompatibility. Moreover, OBPN-1 exhibited 4.5-fold permeation across the rat jejunum compared with 5-FU solution. Thus, the PFGNPs exhibit high DL capacity, sustained delivery, hemocompatibility, improved efficacy, and enhanced permeation profiles compared with 5-FU solution and several other NPs preparations suggesting it is a promising formulation for effective colon cancer control with reduced side effects.


Asunto(s)
Neoplasias del Colon/tratamiento farmacológico , Fluorouracilo/administración & dosificación , Oro/química , Nanopartículas del Metal/química , Animales , Composición de Medicamentos , Sistemas de Liberación de Medicamentos , Liberación de Fármacos , Fluorouracilo/química , Humanos , Ratas , Células Tumorales Cultivadas
17.
Food Chem ; 351: 129292, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33626465

RESUMEN

The detection of carbendazim (CBZ) is important for food safety and human health. However, most current analytical methods require large instruments and highly trained operators. In order to solve this problem, herein, an innovative portable and quantitative photothermal assay platform relying on a thermometer readout for the detection of CBZ has been developed. Gold nanoparticles (AuNPs), which exhibit a strong distance-dependent photothermal effect under specific laser irradiation, were utilized as indicators. The CBZ aptamer was introduced to protect AuNPs from salt-mediated aggregation. When CBZ is present, the binding event between CBZ and aptamer leads to the loss of the aptamer protective effect on AuNPs, and AuNP aggregation occurs. Under 650-nm laser irradiation, the increase in temperature associated with an AuNP-dependent photothermal effect is highly related to the CBZ concentration. Having the advantages of user-friendliness, low cost, quick response, and portability, this method has great potential for on-site applications.


Asunto(s)
Bencimidazoles/análisis , Carbamatos/análisis , Análisis de los Alimentos/métodos , Fungicidas Industriales/análisis , Aptámeros de Nucleótidos/química , Aptámeros de Nucleótidos/metabolismo , Brassica/química , Brassica/metabolismo , Citrus/química , Citrus/metabolismo , Análisis de los Alimentos/instrumentación , Frutas/química , Frutas/metabolismo , Oro/química , Nanopartículas del Metal/química , Espectrofotometría Ultravioleta , Temperatura , Termómetros
18.
Food Chem ; 351: 129238, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33640764

RESUMEN

The colorimetric sensors for reducing sugars based on a redox reaction between AuCl4- ions and fructose, glucose, lactose, or mannose are presented. Gold nanoparticles (AuNPs) that formed at room temperature as a product of this reaction were registered using a spectrophotometer. Lengthening reaction time had a positive effect on the sensitivity of the developed sensors. Different reducing sugars exhibited distinct reaction rates for AuNP formation, with the rate decreasing in the order fructose > glucose > lactose > mannose. LOD values after 60 min of the reaction for different sugars followed the same trend of 0.067, 0.081, 0.087, and 0.106 mM, while LOQ was 0.223, 0.270, 0.289, and 0.353 mM, respectively. The linear range 60 min since the start of the reaction varied from 0.3 up to 5.0 mM for different sugars. The colorimetric sensor was evaluated for use in real samples of beverages, milk, and saliva.


Asunto(s)
Colorimetría/métodos , Oro/química , Nanopartículas del Metal/química , Azúcares/análisis , Análisis de los Alimentos , Límite de Detección , Oxidación-Reducción , Azúcares/química
19.
J Pharm Biomed Anal ; 196: 113924, 2021 Mar 20.
Artículo en Inglés | MEDLINE | ID: mdl-33581588

RESUMEN

Owing to its simplicity and low cost, the lateral flow assay (LFA) is one of the most commonly used point-of-care diagnostic techniques, despite its low sensitivity and poor quantification. Here, we report a newly developed LFA-NanoSuit method (LNSM) combined with a desktop scanning electron microscope (SEM) for the direct observation of immunocomplexes labeled with a colloidal metal instead of signal enhancement strategies, such as using color, electrochemical signals, silver enhancement, magnetic properties, luminescent, and surface-enhanced Raman spectroscopy (SERS). The proposed LNSM suppresses cellulose deformity, thereby allowing the acquisition of high-resolution images of gold/platinum-labeled immunocomplexed pathogens such as influenza A, without conductive treatment as in conventional SEM. Electron microscopy-based diagnosis of influenza A exhibited 94 % clinical sensitivity (29/31; 95 % confidence interval [CI]: 79.3-98.2 %) and 100 % clinical specificity (95 % CI: 98.1-100 %), which was more sensitive (71.4 %) than visual detection (14.3 %), especially in the lower influenza A-RNA copy number group. The detection ability of our method was nearly comparable to that of real-time reverse transcription-PCR. This is the first report on the diagnosis of clinical diseases using LFA equipped with a desktop SEM. This simple and highly sensitive quantitative analysis method involving LFA can be used to diagnose various diseases in humans and livestock, including highly infectious diseases such as COVID-19.


Asunto(s)
Bioensayo/métodos , Oro/química , Nanopartículas del Metal/química , Microscopía Electrónica de Rastreo/métodos , Platino (Metal)/química , Animales , Estudios de Evaluación como Asunto , Humanos , Límite de Detección , Ganado , Pruebas en el Punto de Atención , Espectrometría Raman/métodos
20.
Cell Prolif ; 54(4): e13008, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33634540

RESUMEN

OBJECTIVES: Au nanoclusters (AuNCs) have been used widely in fluorescence bio-imaging because of their good fluorescence, small particle size and non-cytotoxicity. AuNCs are also efficient in computed tomography (CT) imaging. Hence, a dual-modal imaging probe can be constructed without any complicated modification processes by exploiting the excellent performance of AuNCs. In the present study, AuNCs were enriched with mesoporous silica nanoparticles (MSNs) to obtain enhanced fluorescence/CT dual-modal imaging, which was capable of acquiring more imaging information for diseases compared with single-mode imaging. MATERIALS AND METHODS: Biocompatible bovine serum albumin (BSA)-capped AuNCs were prepared and loaded into amine-functionalized MSNs to form MSN@AuNCs. BSA-AuNCs, MSNs, and MSN@AuNCs were characterized by ultraviolet-visible (UV-vis) spectra, transmission electron microscopy (TEM), fluorescence spectra, and zeta potential. CT imaging was recorded using micro-CT scanning. Fluorescence imaging was measured using confocal laser scanning microscopy and flow cytometry. RESULTS: The prepared AuNCs and MSNs possessed good properties as previously reported. The fluorescence intensity and CT value of the AuNCs were enhanced after being enriched with MSNs. The nanoparticles were both non-cytotoxic. Confocal laser scanning microscopy and flow cytometry indicated that MSN@AuNCs in CAL-27 cells showed improved fluorescence imaging compared with simple AuNCs at the same concentration. CONCLUSIONS: The results revealed that the strategy of enriching AuNCs with MSNs can obtain highly sensitive fluorescence/CT dual-modal imaging, which indicated the potential of this nanoparticle in the diagnosis and treatment of disease.


Asunto(s)
Materiales Biocompatibles/química , Oro/química , Microscopía Confocal , Nanopartículas/química , Dióxido de Silicio/química , Microtomografía por Rayos X , Animales , Materiales Biocompatibles/farmacología , Bovinos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Humanos , Ratones , Nanopartículas/toxicidad , Porosidad , Albúmina Sérica Bovina/química
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