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1.
Swiss Med Wkly ; 152: w30102, 2022 01 03.
Artículo en Inglés | MEDLINE | ID: mdl-35019255

RESUMEN

Zoonotic species of the Chlamydiaceae family should be considered as rare pathogenic agents of severe atypical pneumonia. A fatal case of a severe pneumonia due to Chlamydia psittaci was traced back to pet birds, and pneumonia in a pregnant woman was attributed to abortions in a sheep and goat flock, being the source of Chlamydia abortus. The two SARS­CoV­2-negative pneumonia cases presented here were investigated in an inter-disciplinary approach involving physicians and veterinarians. State-of-art molecular methods allowed the identification and genotyping of zoonotic Chlamydiae.


Asunto(s)
COVID-19 , Infecciones por Chlamydia , Chlamydophila psittaci , Animales , Aves , Infecciones por Chlamydia/complicaciones , Infecciones por Chlamydia/diagnóstico , Chlamydophila psittaci/genética , Femenino , Humanos , Embarazo , SARS-CoV-2 , Ovinos
2.
Trop Anim Health Prod ; 54(1): 57, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-35031870

RESUMEN

The loop-mediated isothermal amplification (LAMP) was standardized for rapid detection of Dichelobacter nodosus and Fusobacterium necrophorum. A total of 250 foot swabs were screened from sheep (200) and goats (50) from different districts of Rayalaseema, viz., Chittoor, Nellore, Kadapa, and Anantapur. Out of 250 samples 75 (30.0%) and 85 (34.0%) were positive for D. nodosus and F. necrophorum, respectively. All the 250 samples were screened individually for both the organisms by LAMP. Among them, 104 (41.6%) were found to be positive for D. nodosus and 120 (48.0%) were positive for F. necrophorum. The efficacy of LAMP in terms of sample DNA detection limit was compared with the PCR by using standard dilutions of DNA extracted from D. nodosus and F. necrophorum cultures. The detection limit was found to be higher than PCR for both the organisms. The sensitivity of LAMP is compared with PCR by targeting 16S rRNA gene of D. nodosus and lktA gene of F. necrophorum. In case of D. nodosus, out of 250 samples, 75 (30.0%) were positive by PCR and 104 (41.6%) were positive by LAMP. Among 250 samples, 85 (34.0%) were positive by PCR and 120 (48.0%) were positive by LAMP in case of F. necrophorum. The LAMP was found to be more sensitive than PCR in detecting the organisms with high statistical significance.


Asunto(s)
Infecciones por Fusobacterium , Enfermedades de las Cabras , Infecciones por Bacterias Gramnegativas , Enfermedades de las Ovejas , Animales , Infecciones por Fusobacterium/veterinaria , Enfermedades de las Cabras/diagnóstico , Cabras , Infecciones por Bacterias Gramnegativas/veterinaria , Técnicas de Diagnóstico Molecular , Técnicas de Amplificación de Ácido Nucleico , ARN Ribosómico 16S/genética , Estándares de Referencia , Ovinos , Enfermedades de las Ovejas/diagnóstico
3.
Trop Anim Health Prod ; 54(1): 56, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-35031883

RESUMEN

This study investigated the effects of bael leaf powder (BLP) on growth performance, immunity, serum biochemistry, and caecal microbiology of broiler chickens. Following completely randomised design, a total of 288-day-old CARIBRO-Vishal broiler chickens were randomly divided into six treatment groups consisting of experimental diets formulated as follows - T1 (basal diet), T2 (basal diet + 250 mg bacitracin methylene disalicylate-BMD/kg diet), T3 (basal diet + 5 g BLP/kg diet) T4 (basal diet + 10 g BLP/kg diet), T5 (basal diet + 15 g BLP/kg diet), and T6 (basal diet + 20 g BLP/kg diet). Proximate analysis of green bael leaves revealed 65.6% moisture and on dry matter basis, BLP contained 14.31% protein, 1.89% fat, 16.30% crude fibre, 4.25% calcium, and 2.08% phosphorous. This study revealed no adverse effects of BLP supplementation on growth performance and carcass characteristics of broiler chicken. The BLP supplementation at 20 g/kg diet resulted in higher relative weights of bursa and thymus with better cell mediated (higher foot web index) and humoral immune (higher antibody titre against sheep RBCs) responses in broiler chicken. Lower abdominal fat deposition was observed in broiler chicken fed 20 g BLP/kg diet. Significantly lower serum uric acid, creatinine, AST, and ALT were observed in BLP supplemented chicken which indicates nephro-protective and hepato-protective functions of BLP. Serum cholesterol, triglycerides, and tissue cholesterol content of broiler chicken decreased progressively with increasing dietary BLP levels. The supplementation of 20 g BLP/kg diet resulted in lower E. coli and Coliform counts, whereas, increasing trend was observed in Lactobacillus count in caecum of broiler chicken. In conclusion, the BLP supplementation at 20 g/kg diet exerted immunomodulatory, anti-hyperlipidaemic, and antibacterial effects in broiler chicken without any adverse effects on the growth performance.


Asunto(s)
Aegle , Pollos , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Antibacterianos , Dieta/veterinaria , Suplementos Dietéticos/análisis , Escherichia coli , Hojas de la Planta , Polvos , Ovinos , Ácido Úrico
4.
Anal Chim Acta ; 1191: 339330, 2022 Jan 25.
Artículo en Inglés | MEDLINE | ID: mdl-35033236

RESUMEN

Capripoxvirus (CaPV) is one of the common skin diseases infecting cattle and sheep which can cause serious economic losses. Establishing ultra-sensitive, rapid, and point-of-care detection of CaPV is particularly important for hindering its spread. Here, we use the principle that CRISPR/Cpf1 can specifically recognize the target DNA and activate its trans-cleavage activity to identify the CaPV product amplified by loop-mediated amplification (LAMP). Under the designed specific primers, a set of LAMP which can amplify CaPV specifically was established and optimized firstly. Then, the CRISPR/Cpf1 was introduced to identify LAMP products. LAMP can be completed at a constant temperature, thus avoiding the use of temperature-variable instruments, making it possible to detect viruses outside the laboratory. To further satisfy the point-of-care detection of CaPV, we introduced a portable fluorometer and CRISPR-based lateral flow test. Due to the introduction of CRISPR/Cpf1, the sensitivity of the method is greatly increased, which is of great significance for the early detection of viruses. Through CRISPR/Cpf1-mediated fluorescence detection, we can detect CaPV as low as 1.47 × 10-3 TCID50 in 50 min, 1000 times more sensitive than quantitative real-time PCR. Through CRISPR-based lateral flow test, we can visually detect CaPV as low as 1.47 × 10-2 TCID50. Besides, this strategy can be used for the primary samples obtained from the cell culture of CaPV after simple ultrasonic disruption, which eliminates the complicated nucleic acid extraction steps required by traditional methods.


Asunto(s)
Capripoxvirus , Animales , Capripoxvirus/genética , Bovinos , Cartilla de ADN , Técnicas de Amplificación de Ácido Nucleico , Sistemas de Atención de Punto , Sensibilidad y Especificidad , Ovinos
5.
Anal Chim Acta ; 1191: 339291, 2022 Jan 25.
Artículo en Inglés | MEDLINE | ID: mdl-35033276

RESUMEN

The pregnancy-associated glycoproteins (PAGs) have been widely used as biomarkers for the early diagnosis of pregnancy in cattle and sheep. This study aimed to obtain the single-stranded DNA aptamers that specifically bound to ovine pregnancy-associated glycoprotein 7 (ovPAG7) with high affinity (Kd = 9.8-32.4 nM) using real serum sample-assisted FluMag-systematic evolution of ligands by exponential enrichment (SELEX). Subsequently, the selected aptamers were applied to fabricate an ultrasensitive colorimetric aptasensor for ovPAG7 detection based on functionalized magnetic microparticles and hybridization chain reaction. Under the optimized conditions, the colorimetric aptasensor exhibited a broad linear range (0.2-500 ng mL-1), low detection limit (0.081 ng mL-1), good recovery rate (94.5-109.1%), and high repeatability (relative standard deviation of 4.02-8.16%) in ovPAG7-spiked serum. Furthermore, this aptasensor was applied to measure the ovPAG7 in serum samples of ewes for pregnancy diagnosis. Blood samples were collected from Chinese Merino ewes on days 22, 28 after artificial insemination (AI) for ovPAG7 detection, respectively. Transrectal ultrasonography diagnosis of pregnancy 45 days after AI was the reference (gold) standard for all PAG tests. Diagnostic sensitivity, specificity, and accuracy of the proposed aptasensor were 95.8, 87.5, and 92.5% at day 22 and 95.8, 90.6, and 93.7% at day 28, respectively. The degree of agreement (Kappa) between developed aptasensor and ultrasonography diagnosis 22 and 28 day after AI were higher than 0.8. These results illustrated that the aptasensor was proved to be a sensitive, reliable and cost-effective way of measuring PAG and might be a useful means of pregnancy detection in ewes.


Asunto(s)
Aptámeros de Nucleótidos , Técnica SELEX de Producción de Aptámeros , Animales , Bovinos , Colorimetría , Femenino , Glicoproteínas , Límite de Detección , Fenómenos Magnéticos , Embarazo , Ovinos
6.
Trop Anim Health Prod ; 54(1): 61, 2022 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-35037142

RESUMEN

The objective of this study was to investigate the effects of rumen-protected lysine (RPL) and methionine (RPM) supplementation on production performance of nursing ewes fed two levels of dietary protein. Individually housed Awassi ewes (n = 34) nursing single lambs were randomly assigned (2 × 2 factorial design) to one of four dietary treatments with two levels of protein (170 or 151 g/kg; HP or MP) and two levels of RPL and RPM (0 or 8.5 plus 4 g/day/ewe of RPL and RPM, respectively; no or yes). The trial lasted for 5 weeks. Ewes fed the MP diets had (P < 0.01) lower protein intake compared to those fed the HP diets. Intake of other nutrients and milk composition were not significantly (P > 0.13) affected by dietary treatments. Ewes fed the MPYES diet produced more (P < 0.05) milk compared to those fed the MPNO and HPYES diets and tended (P = 0.08) to be more than the HPNO diet. Additionally, milk composition yields for the MPYES diet were significantly (P < 0.05) more than the HPYES diets and tended (P ≤ 0.10) to be more than the MPNO and HPNO diets. Milk efficiency was highest (P < 0.05) for the MPYES diet. Final BW, total gain, and growth rate of lambs were greater when their dams were fed the MPYES diet compared to MPNO and HPNO diets. Under our conditions, decreasing dietary protein from 170 to 151 g/kg did not negatively affect the performance of ewes and their lambs. Supplemental RPL and RPM were beneficial for ewes fed diets containing 151 g/kg, but not 170, protein.


Asunto(s)
Lisina , Metionina , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Proteínas en la Dieta , Suplementos Dietéticos , Femenino , Lactancia , Leche , Rumen , Ovinos , Oveja Doméstica
7.
Trop Anim Health Prod ; 54(1): 62, 2022 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-35037143

RESUMEN

Brucellosis is a zoonotic infectious disease with a worldwide distribution. This cross-sectional study aimed to determine the occurrence of Brucella in milk and blood samples of ruminants and the importance factors associated with animal brucellosis in Eastern Iran. A total of 200 paired samples, including blood (100) and milk (100), were obtained from the goats, sheep, and cows in Eastern Iran. Serum agglutination (SAT) and 2-mercapto ethanol (2-ME) tests were performed on the sera. A multiplex-polymerase chain reaction (m-PCR) assay was performed to identify the following species of Brucella, including B. abortus biovar 1, 2, 4, 3b, 5, 6, and 9, B. abortus S19, B. melitensis, and B. melitensis Rev.1 vaccine strain. B. abortus RB51 vaccine strain was also investigated in a second PCR assay. Risk factors for infection with Brucella spp. including the effect of abortion, contact with the wild animals, herd type, age, and previous vaccination in predicting blood contamination with B. abortus biovar 1, 2, and 4 were modeled by use of the artificial neural network. A total of 23 samples were seropositive regarding SAT and 2-ME tests. In total, B. abortus was detected in 35% and 15% of blood and milk samples, respectively, by the m-PCR assay. One sample of each of milk and blood was detected to have B. melitensis. Some samples were simultaneously contaminated with two Brucella species or two biovars of B. abortus. B. abortus S19 and B. melitensis Rev.1 vaccine strains were also detected in milk and blood samples. The sensitivity and specificity of the ANN model were 81% and 62%, respectively. In conclusion, B. abortus had higher frequency than B. melitensis in blood and milk samples. ANN determined herd type, previous vaccination, and age of the animal as the largest predictors of blood contamination with B. abortus.


Asunto(s)
Brucella melitensis , Brucelosis , Enfermedades de los Bovinos , Enfermedades de las Cabras , Animales , Brucella abortus/genética , Brucella melitensis/genética , Brucelosis/epidemiología , Brucelosis/veterinaria , Bovinos , Estudios Transversales , Femenino , Enfermedades de las Cabras/epidemiología , Reacción en Cadena de la Polimerasa Multiplex/veterinaria , Redes Neurales de la Computación , Embarazo , Factores de Riesgo , Ovinos
8.
Trop Anim Health Prod ; 54(1): 42, 2022 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-35013875

RESUMEN

A study was carried out to evaluate the performance and ruminal and intestinal morphology of Santa Inês sheep subjected to feed restriction followed by refeeding. A total of 40 uncastrated lambs with an approximate age of 120 ± 15 days and mean body weight (BW) of 17.04 ± 1.18 kg were randomly divided into two groups of BW (20 and 25 kg of BW), which were subjected to different levels of feed restriction (0%, 25%, and 40% of feed restriction). For performance variables, six treatments were considered (0, 25%, and 40% of feed restriction for both groups (20 and 25 kg of BW)) and five treatments for morphometric variables (ad libitum, 25% and 40% for both groups (20 and 25 kg of BW)). All animals were slaughtered with 14 weeks of experimentation. During the feed restriction phase, the dry matter intake (DMI), feed efficiency (FE), and average daily gain (ADG) decreased (P < 0.05) as the level of restriction increased. During the refeeding phase, lambs with 20 kg of body weight subjected to restriction presented lower (P < 0.05) DMI in the ad libitum treatment. However, lambs with 25 kg of body weight under feed restriction presented DMI, FE, and ADG similar (P > 0.05) to the group ad libitum. The final body weight of restricted lambs after refeeding (both groups 20 and 25 kg of body weight) was lower (P < 0.05) than lambs feed ad libitum. In relation to morphology, restricted lambs showed greater height ruminal papillae and larger (P < 0.05) area of ruminal absorption and intestinal absorption, especially the lambs under treatment 40% of feed restriction. The feed restriction followed by refeeding in sheep provided partial compensatory gain, in addition, caused morphological changes in the rumen and intestine that allowed greater absorption and possibly compensatory gain in periods of greater refeeding.


Asunto(s)
Alimentación Animal , Dieta , Alimentación Animal/análisis , Animales , Peso Corporal , Intestinos , Ovinos
9.
Trop Anim Health Prod ; 54(1): 49, 2022 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-35020037

RESUMEN

This study was aimed to assess the impact-enriched Saccharomyces cerevisiae with organic selenium addition in finishing lambs on fatty acid composition and physicochemical meat characteristics. Eighteen five-month-old Pelibuey female lambs were fed the same diet for 60 days. Animals were assigned a completely random design of three treatments, control (Se0) without the addition of selenium-yeast or supplemented with 0.35 ppm of selenium-yeast (Se35) and with 0.60 ppm of selenium-yeast (Se60). Lambs were slaughtered at an average weight of 39.5 ± 4.41 kg. Feed intake and meat water holding capacity were decreased (P = 0.001) in Se35 lambs, whereas meat moisture and fat were decreased (P = 0.002) in Se60 lambs. However, meat carbohydrates were increased (P = 0.001) in Se60 lambs. It is concluded that consumption of selenium-yeast in lambs did not alter the productive variables nor the fatty acid composition, though, the fat content is lower, and the carbohydrates are higher in physicochemical meat characteristics.


Asunto(s)
Selenio , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Suplementos Dietéticos , Femenino , Carne/análisis , Saccharomyces cerevisiae , Ovinos , Oveja Doméstica
10.
Trop Anim Health Prod ; 54(1): 39, 2022 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-35006405

RESUMEN

One of the projected effects of climate change is a reduction in rainfall in certain regions of the world. Hence, the agricultural and livestock sectors will have to cope with increasing incidences of water shortage while still maintaining productivity levels to feed an ever increasing global population. This short communication reports on the effect of a 2-week water stress on Pelibuey sheep in Cuba. Three treatments were compared, viz. supply of water ad libitum, water supplied once every 3 or 6 days. Following exposure to the water stress, the results showed no changes in sheep body weight or rectal temperature. However, respiration frequency was affected with water stress causing a reduction from 23.3 to 13.3 respirations per min in control and water-deprived animals, respectively. Furthermore, there was evidence for hemoconcentration in response to water stress (levels of hemoglobin increased from 9.2 to 13.1 g L-1 and hematocrits from 27.6 to 39.3% in the control group and animals restricted to water once every 6 days. The imposed water stress was also evident in the reduction of lymphocytes (from ±63 to 43%), and in increase of neutrophils (from approximately 38 to 54%) and leukocytes (from 3133 to 4933 per mm3). The results indicated a decline in the levels of antioxidants, i.e., SOD from approximately 13 to 10 U mg-1 protein and CAT activity from 23 to 9 U mg-1 protein. To the best of our knowledge, this is the first report on the response of Pelibuey sheep to short-term water shortage stress under Cuban environmental conditions.


Asunto(s)
Cambio Climático , Enfermedades de las Ovejas , Animales , Antioxidantes , Ganado , Frecuencia Respiratoria , Ovinos
11.
Vet Rec ; 190(1): 28-29, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-34994428

RESUMEN

This focus article has prepared by Rachael Collins and Amanda Carson of the APHA Small Ruminant Expert Group.


Asunto(s)
Enfermedades de la Boca , Enfermedades de las Ovejas , Animales , Enfermedades de la Boca/veterinaria , Rumiantes , Ovinos , Enfermedades de las Ovejas/epidemiología , Oveja Doméstica
13.
Vet Rec ; 190(1): 13, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-34994444
14.
BMC Genomics ; 23(1): 23, 2022 Jan 04.
Artículo en Inglés | MEDLINE | ID: mdl-34983377

RESUMEN

BACKGROUND: South Africa and Australia shares multiple important sheep breeds. For some of these breeds, genomic breeding values are provided to breeders in Australia, but not yet in South Africa. Combining genomic resources could facilitate development for across country selection, but the influence of population structures could be important to the compatability of genomic data from varying origins. The genetic structure within and across breeds, countries and strains was evaluated in this study by population genomic parameters derived from SNP-marker data. Populations were first analysed by breed and country of origin and then by subpopulations of South African and Australian Merinos. RESULTS: Mean estimated relatedness according to the genomic relationship matrix varied by breed (-0.11 to 0.16) and bloodline (-0.08 to 0.06) groups and depended on co-ancestry as well as recent genetic links. Measures of divergence across bloodlines (FST: 0.04-0.12) were sometimes more distant than across some breeds (FST: 0.05-0.24), but the divergence of common breeds from their across-country equivalents was weak (FST: 0.01-0.04). According to mean relatedness, FST, PCA and Admixture, the Australian Ultrafine line was better connected to the SA Cradock Fine Wool flock than with other AUS bloodlines. Levels of linkage disequilibrium (LD) between adjacent markers was generally low, but also varied across breeds (r2: 0.14-0.22) as well as bloodlines (r2: 0.15-0.19). Patterns of LD decay was also unique to breeds, but bloodlines differed only at the absolute level. Estimates of effective population size (Ne) showed genetic diversity to be high for the majority of breeds (Ne: 128-418) but also for bloodlines (Ne: 137-369). CONCLUSIONS: This study reinforced the genetic complexity and diversity of important sheep breeds, especially the Merino breed. The results also showed that implications of isolation can be highly variable and extended beyond breed structures. However, knowledge of useful links across these population substructures allows for a fine-tuned approach in the combination of genomic resources. Isolation across country rarely proved restricting compared to other structures considered. Consequently, research into the accuracy of across-country genomic prediction is recommended.


Asunto(s)
Genética de Población , Genómica , Oveja Doméstica/genética , Animales , Australia , Cruzamiento , Genotipo , Desequilibrio de Ligamiento , Ovinos/genética , Sudáfrica
15.
Vet Parasitol ; 301: 109640, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34973595

RESUMEN

Infection caused by gastrointestinal nematodes is an important issue for animal health and production. Controlling worm infections improves the sustainability of the sheep industry. Genetic selection of animals that are resistant to gastrointestinal nematodes is another strategy to render sheep production more sustainable by decreasing the use of anthelmintics. The aims of this study were (1) to explore the additive-genetic pattern of EBVs for Famacha© (FAM), packed-cell volume (PVC), and fecal egg counts (FEC) of Santa Ines sheep, (2) to propose a classification of animals that are resistant, resilient and susceptible to gastrointestinal nematodes based on their additive-genetic patterns, and (3) to identify the most suitable animals for selection based on their genetic pattern. A dataset of 2,241 records from 747 animals was used to predict the breeding values for indicator traits of resistance to gastrointestinal nematodes with THRGIBBS1F90 and to carry out cluster analyses was used R software. Three clusters of animals were found in the population using hierarchical cluster analysis of the breeding values for FAM, PCV and FEC. Each cluster was characterized by different additive-genetic patterns identified by k-means non-hierarchical cluster analysis. Among a total of 747 animals, 196 were classified as resistant, 288 as resilient, and 263 as susceptible. Cluster analysis is a valuable tool for data screening that permits to evaluate only selection candidates based on their additive-genetic pattern for gastrointestinal nematode resistance. EBVs for FEC were decisive to divide the population into resilient, resistant and susceptible animals. It is also important to include the EBVs for PCV and FAM to adequately distinguish resistant from resilient animals. Finally, the resistant cluster consisted of the most desirable animals to be used as selection candidates in order to genetically improve resistance to infection with gastrointestinal nematodes. This cluster contained animals with the most appropriate additive-genetic pattern to achieve the breeding goal, with positive breeding values for PCV and negative breeding values for FAM and FEC.


Asunto(s)
Hemoncosis , Haemonchus , Nematodos , Infecciones por Nematodos , Enfermedades de las Ovejas , Animales , Análisis por Conglomerados , Susceptibilidad a Enfermedades/veterinaria , Heces , Hemoncosis/veterinaria , Nematodos/genética , Infecciones por Nematodos/genética , Infecciones por Nematodos/veterinaria , Recuento de Huevos de Parásitos/veterinaria , Ovinos , Enfermedades de las Ovejas/genética
16.
Vet Parasitol ; 301: 109641, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34979475

RESUMEN

Ovine neosporosis, caused by the Apicomplexan parasite Neospora caninum, leads to reproductive failure worldwide. Nowadays, there is a trend to develop diagnostic techniques using non-invasive samples, such as milk, in order to reduce animal stress, sample collection effort, and costs. The objective of this study was to develop and validate a highly sensitive and specific serological technique, based on a time resolved-fluorescence immunoassay using a N. caninum GRA7 antigen (GRA7-TRFIA), for the detection of anti-N. caninum immunoglobulins G on sheep' full-cream milk samples. An analytical validation was performed, including intra- and inter-assay precision, analytical sensitivity and accuracy. The diagnostic performance of the assay was evaluated by studying the positive-negative discrimination by Mann Whitney U tests. In additon optimal cut-offs, diagnostic sensitivity and specificity, and areas under the curve were calculated by three Receiver Operating Curve (ROC) analyses, using GRA7-TRFIA and a N. caninum tachyzoite soluble extract-based ELISA (NcSALUVET-ELISA) in blood sera, and the coinciding results of both techniques, as reference techniques. Moreover, Spearman's correlation of GRA7-TRFIA in milk with the techniques in sera and agreement (kappa values) were also estimated. GRA7-TRFIA for milk samples showed an adequate precision, with high analytical sensitivity and accuracy. Regarding ROC analyses, at the optimal cut-offs, the diagnostic sensitivity and specificity were more than 90 % in all cases. In addition, GRA7-TRFIA values in milk were more positively correlated to GRA7-TRFIA values in blood sera than in the case of values obtained with NcSALUVET-ELISA. GRA7-TRFIA in milk showed an almost perfect agreement with GRA7-TRFIA in blood sera (kappa = 0.98) and with the coinciding results of GRA7-TRFIA and NcSALUVET in blood sera (kappa = 1.00), while it has a substantial agreement with NcSALUVET-ELISA (kappa = 0.69). In the light of these results, GRA7-TRFIA in full-cream milk samples is a highly sensitive technique that could be used for screening anti-N. caninum antibodies in sheep flocks.


Asunto(s)
Enfermedades de los Bovinos , Coccidiosis , Neospora , Enfermedades de las Ovejas , Animales , Anticuerpos Antiprotozoarios , Bovinos , Coccidiosis/diagnóstico , Coccidiosis/veterinaria , Ensayo de Inmunoadsorción Enzimática/veterinaria , Leche , Sensibilidad y Especificidad , Ovinos , Enfermedades de las Ovejas/diagnóstico
17.
Trop Anim Health Prod ; 54(1): 40, 2022 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-35006384

RESUMEN

To study the efficacy of organic trace mineral supplementation on blood parameters, digestibility, and growth as compared to inorganic sources, 18 Zandi male lambs (with initial body weight, 28.5 ±1.4 kg and 110±5 days old) were divided into three groups of six animals in each in a completely randomized design. Lambs in the control group were fed basal diet containing 63 kg/100 kg of concentrate mixture, 22 kg/100 kg of alfalfa hay, and 15 kg/100 kg wheat straw. Animals in the experimental groups were additionally supplemented with trace minerals supplied by sulfates or a diet in which 25.7 ppm Zn, 14.3 ppm Mn, 8.9 ppm Cu from mineral-amino acid complex, and 0.86 ppm Co from Co glucoheptonate replaced with similar amounts of Zn, Mn, Cu, and Co from sulfates. All lambs were kept in individual pens with cemented floor and provision of individual feeding and watering. Lambs fed with either organic or inorganic trace mineral supplement showed higher dry matter intake and growth rate and better feed conversion efficiency (P<0.05) as compared to the control group. Blood glucose, urea nitrogen, cholesterol, and hepatic enzymes were similar among the treatments. Triglycerides (P<0.01) concentration was lower for mineral-supplemented groups. Blood vitamin B12 concentration increased with mineral supplementation and was higher for the lambs fed with organic source of trace elements as compared with those fed with inorganic mineral and the control diet (P=0.04). The results of this study showed that feeding organic trace elements improves growth performance of finishing lambs but did not affect nutrient digestibility and blood parameters.


Asunto(s)
Oligoelementos , Alimentación Animal/análisis , Animales , Cobre , Dieta , Suplementos Dietéticos , Masculino , Ovinos , Zinc
18.
BMC Infect Dis ; 22(1): 43, 2022 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-35012454

RESUMEN

BACKGROUND: Brucellosis is an infectious disease caused by Brucella spp, which can involve the cardiovascular, digestive, and respiratory systems. Cardiovascular involvement is a rare occurrence, it has an extremely high mortality rate. CASE PRESENTATION: A 67-year-old Chinese man presented with thoracic aortic multiple ulcers and partial aneurysm formation that caused symptoms of left waist and left buttock pain. The man was admitted to our hospital due to abdominal aorta pseudoaneurysms 5 years ago. The diagnosis was made by thoracic computed tomography angiography (CTA), previous history, and positive culture of Brucella, and the patient was successfully treated by thoracic aortic covered stent-graft implantation and specific medical treatment. CONCLUSIONS: People who have a history of contact with cattle and sheep, should beware of the possibility of Brucella infection. If chest and abdominal pain occur, timely medical treatment is recommended, aortic aneurysm, the disease with a high risk of death, can be identified or excluded by CTA. Early treatment and prevention of disease progression are more beneficial to patients.


Asunto(s)
Aneurisma de la Aorta , Implantación de Prótesis Vascular , Brucelosis , Animales , Aorta Torácica , Prótesis Vascular , Bovinos , Humanos , Recurrencia , Ovinos , Stents , Resultado del Tratamiento , Úlcera
19.
Rev Bras Parasitol Vet ; 31(1): e017421, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35019027

RESUMEN

The present study aimed to perform an epidemiological and morphological identification of Eimeria infection in sheep in Brazil. Fecal samples from sheep were collected from 20 farms in northern Paraná, Brazil. An epidemiological questionnaire was used to evaluate the risk factors. Fecal samples containing oocysts per gram of feces (OoPG) ≥1000 were subjected to the modified Willis-Mollay method to perform oocyst identification. Sporulated oocysts were observed microscopically for morphological identification. A total of 807 fecal samples were collected. Based on the morphological characteristics of the sporulated oocysts, 10 species of Eimeria were identified, with main species observed: Eimeira ovinoidalis (98.1%), Eimeria crandallis (87.6%), Eimeria parva (79.1%), and Eimeria bakuensis (60.8%). Only 2.6% (7/268) of the sheep were infected with a single species, 4.8% (13/268) contained two different species, and 92.5% (248/268) were infected with three or more species. The analysis of risk factors showed that an intensive rearing, no rotation of pasture, dirt, and slatted floors, and age up to 12 months were associated with infection. This study showed a high prevalence of Eimeria natural infection in sheep from northern Paraná, Brazil. Furthermore, based on the risk factors, good management and hygiene practices must be employed to avoid infection.


Asunto(s)
Coccidiosis , Eimeria , Enfermedades de las Ovejas , Animales , Brasil/epidemiología , Coccidiosis/epidemiología , Coccidiosis/veterinaria , Heces , Prevalencia , Factores de Riesgo , Ovinos , Enfermedades de las Ovejas/epidemiología
20.
Genet Sel Evol ; 54(1): 2, 2022 Jan 03.
Artículo en Inglés | MEDLINE | ID: mdl-34979909

RESUMEN

BACKGROUND: Gastroinestinal nematodes (GIN) are one of the major health problem in grazing sheep. Although genetic variability of the resistance to GIN has been documented, traditional selection is hampered by the difficulty of recording phenotypes, usually fecal egg count (FEC). To identify causative mutations or markers in linkage disequilibrium (LD) to be used for selection, the detection of quantitative trait loci (QTL) for FEC based on linkage disequilibrium-linkage analysis (LDLA) was performed on 4097 ewes (from 181 sires) all genotyped with the OvineSNP50 Beadchip. Identified QTL regions (QTLR) were imputed from whole-genome sequences of 56 target animals of the population. An association analysis and a functional annotation of imputed polymorphisms in the identified QTLR were performed to pinpoint functional variants with potential impact on candidate genes identified from ontological classification or differentially expressed in previous studies. RESULTS: After clustering close significant locations, ten QTLR were defined on nine Ovis aries chromosomes (OAR) by LDLA. The ratio between the ANOVA estimators of the QTL variance and the total phenotypic variance ranged from 0.0087 to 0.0176. QTL on OAR4, 12, 19, and 20 were the most significant. The combination of association analysis and functional annotation of sequence data did not highlight any putative causative mutations. None of the most significant SNPs showed a functional effect on genes' transcript. However, in the most significant QTLR, we identified genes that contained polymorphisms with a high or moderate impact, were differentially expressed in previous studies, contributed to enrich the most represented GO process (regulation of immune system process, defense response). Among these, the most likely candidate genes were: TNFRSF1B and SELE on OAR12, IL5RA on OAR19, IL17A, IL17F, TRIM26, TRIM38, TNFRSF21, LOC101118999, VEGFA, and TNF on OAR20. CONCLUSIONS: This study performed on a large experimental population provides a list of candidate genes and polymorphisms which could be used in further validation studies. The expected advancements in the quality of the annotation of the ovine genome and the use of experimental designs based on sequence data and phenotypes from multiple breeds that show different LD extents and gametic phases may help to identify causative mutations.


Asunto(s)
Parásitos , Animales , Femenino , Ligamiento Genético , Genómica , Desequilibrio de Ligamiento , Polimorfismo de Nucleótido Simple , Sitios de Carácter Cuantitativo , Ovinos/genética
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