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1.
Arthroscopy ; 37(4): 1141-1142, 2021 04.
Artículo en Inglés | MEDLINE | ID: mdl-33812518

RESUMEN

Although the clinical impact of positive cultures at the time of primary shoulder surgery remain unknown, much effort has been placed on identifying agents for skin preparation that reduce Cutibacterium acnes skin colonization. Although several randomized controlled trials of hydrogen peroxide use as part of the skin preparation exist, they are plagued by small sample sizes that lead to inadequate power or statistical fragility. Despite the lack of perfect data, our clinical experience and break-even analyses indicate value to routine use of hydrogen peroxide as part of the perioperative skin preparation prior to shoulder surgery.


Asunto(s)
Infecciones por Bacterias Grampositivas , Articulación del Hombro , Humanos , Peróxido de Hidrógeno , Propionibacterium acnes , Manguito de los Rotadores , Hombro/cirugía , Articulación del Hombro/cirugía , Piel , Infección de la Herida Quirúrgica , Suturas
2.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 33(3): 344-348, 2021 Mar.
Artículo en Chino | MEDLINE | ID: mdl-33834978

RESUMEN

OBJECTIVE: To explore whether microRNA-21-5p (miR-21-5p) has the effect of anti-apoptosis of human alveolar type II epithelial cells (AT II). METHODS: AT II cells derived from the human were cultured in vitro and used for experiments when the cells were grown until the presence of lamellar bodies and microvilli were observed by light microscope. The cells were divided into blank control group (direct culture), hydrogen peroxide (H2O2) injury group (cultured with 0.5 mmol/L H2O2), and miR-21-5p overexpression group (using miR-21-5p with a multiplicity of infection (MOI) of 100 lentiviral overexpression vector with 0.5 mmol/L H2O2) and miR-21-5p empty virus control group (miR-21-5p lentiviral blank vector was co-cultured with 0.5 mmol/L H2O2). In each group, cell proliferation was detected by cell counting kit-8 (CCK-8) at 0, 12, 24, 36, and 48 hours of cell culture; cell apoptosis was detected by flow cytometry at 24 hours of culture. RESULTS: (1) Cell proliferation activity test results: with the extension of cell culture time, the cell proliferation activity of the blank control group gradually increased, while the cell proliferation activity gradually decreased after the addition of 0.5 mmol/L H2O2. However, the cells proliferation activity in the miR-21-5p overexpression group decreased more slowly than that in the H2O2 injury group and the miR-21-5p empty virus control group, and the cell proliferation activity at 48 hours was significantly higher than the H2O2 injury group and the miR-21-5p empty virus control group (A value: 0.295±0.005 vs. 0.184±0.005, 0.169±0.002, both P < 0.05). It showed that both H2O2 and lentivirus accelerated cell damage, while miR-21-5p could reduce cell apoptosis. (2) Apoptosis rate test results: compared with the blank control group, the apoptosis rate increased significantly after adding 0.5 mmol/L H2O2; while the apoptosis rate of the miR-21-5p overexpression group was lower than that of the H2O2 injury group and miR-21-5p empty virus control group [early apoptosis rate: (14.31±0.12)% vs. (24.50±0.12)%, (23.41±0.13)%; late apoptosis rate: (8.12±0.13)% vs. (9.71±0.11)%, (10.41±0.15)%; overall apoptosis rate: (22.33±0.12)% vs. (34.21±0.10)%, (33.82±0.14)%; all P < 0.05], which further proved that miR-21-5p had anti-apoptotic effects. CONCLUSIONS: miR-21-5p has an anti-apoptotic effect on human AT II.


Asunto(s)
Células Epiteliales Alveolares , MicroARNs , Apoptosis , Proliferación Celular , Humanos , Peróxido de Hidrógeno , MicroARNs/genética
3.
Pestic Biochem Physiol ; 174: 104824, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33838717

RESUMEN

Acetamiprid is a new type of nicotinic insecticide that is widely used in pest control. Its environmental residues may cause silkworm cocooning disorder. In this study, silkworms that received continuous feeding of low concentration acetamiprid (0.15 mg/L) showed significantly decreased silk gland index and cocooning rate. Gene expression profiling of posterior silk glands (PSGs) revealed that the differentially expressed genes were significantly enriched in oxidative stress-related signal pathways with significant up-regulation. The contents of both H2O2 and MDA were increased, along with significantly elevated SOD and CAT activities, all of which reached maximal values at 48 h when H2O2 and MDA's contents were 10.46 and 7.98 nmol/mgprot, respectively, and SOD and CAT activities were 5.51 U/mgprot and 33.48 U/gprot, respectively. The transcription levels of antioxidant enzyme-related genes SOD, Mn-SOD, CuZn-SOD, CAT, TPX and GPX were all up-regulated, indicating that exposure to low concentration acetamiprid led to antioxidant response in silkworm PSG. The key genes in the FoxO/CncC/Keap1 signaling pathway that regulates antioxidant enzyme activity, FoxO, CncC, Keap1, NQO1, HO-1 and sMaf were all up-regulated during the whole process of treatment, with maximal values being reached at 72 h with 2.91, 1.46, 1.82, 2.52, 2.32 and 4.01 times of increases, respectively. These results demonstrate that exposure to low concentration acetamiprid causes oxidative stress in silkworm PSG, which may be the cause of cocooning disorder in silkworm. Our study provides a reference for the safety evaluation of environmental residues of acetamiprid on non-target insects.


Asunto(s)
Bombyx , Animales , Bombyx/genética , Bombyx/metabolismo , Crecimiento y Desarrollo , Peróxido de Hidrógeno , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Proteína 1 Asociada A ECH Tipo Kelch , Factor 2 Relacionado con NF-E2/metabolismo , Neonicotinoides , Estrés Oxidativo , Seda
4.
PLoS One ; 16(3): e0248487, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33735216

RESUMEN

A gas-phase Advanced Oxidation Process (gAOP) was evaluated for decontaminating N95 and surgical masks. The continuous process was based on the generation of hydroxyl-radicals via the UV-C (254 nm) photo-degradation of hydrogen peroxide and ozone. The decontamination efficacy of the gAOP was dependent on the orientation of the N95 mask passing through the gAOP unit with those positioned horizontally enabling greater exposure to hydroxyl-radicals compared to when arranged vertically. The lethality of gAOP was independent of the applied hydrogen peroxide concentration (2-6% v/v) but was significantly (P<0.05) higher when H2O2 was introduced into the unit at 40 ml/min compared to 20 ml/min. A suitable treatment for N95 masks was identified as 3% v/v hydrogen peroxide delivered into the gAOP reactor at 40 ml/min with continuous introduction of ozone gas and a UV-C dose of 113 mJ/cm2 (30 s processing time). The treatment supported >6 log CFU decrease in Geobacillus stearothermophilus endospores, > 8 log reduction of human coronavirus 229E, and no detection of Escherichia coli K12 on the interior and exterior of masks. There was no negative effect on the N95 mask fitting or particulate efficacy after 20 passes through the gAOP system. No visual changes or hydrogen peroxide residues were detected (<1 ppm) in gAOP treated masks. The optimized gAOP treatment could also support >6 log CFU reduction of endospores inoculated on the interior or exterior of surgical masks. G. stearothermophilus Apex spore strips could be applied as a biological indicator to verify the performance of gAOP treatment. Also, a chemical indicator based on the oxidative polymerization of pyrrole was found suitable for reporting the generation of hydroxyl-radicals. In conclusion, gAOP is a verifiable treatment that can be applied to decontaminate N95 and surgical masks without any negative effects on functionality.


Asunto(s)
Descontaminación/métodos , Máscaras/virología , Gases/química , Humanos , Peróxido de Hidrógeno/química , Radical Hidroxilo/química , Oxidación-Reducción , Ozono/química , Fotólisis
5.
Sci Total Environ ; 772: 145554, 2021 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-33770853

RESUMEN

To understand the effect mechanisms of iron plaque and fatty acids on the migration of PBDEs from soil to rice (Oryza sativa), pot experiments were conducted in the soil spiked with decabromodiphenyl ether (BDE-209) under the conditions of tourmaline and nano-goethite Fenton-like treatments. The results showed that iron mineral Fenton-like oxidation could effectively remove BDE-209 from rhizosphere soil, the highest removal rate obtained 89.29% with the addition of 0.4 mmol/L H2O2 and 8 g nano-goethite (G + 3H group). Iron mineral Fenton-like oxidation could produce iron plaque (IP) on rice roots and accumulate a part of contaminants on the surface of IP, further weakening BDE-209 uptake in the plants. Additionally, the occurrence of fatty acid variation induced by BDE-209 stress, iron mineral Fenton-like oxidation at high concentrations of H2O2 with 0.4 mmol/L affected the distribution of fatty acids in plant tissues, especially for C18:0 fatty acid. While the IP on rice roots prevented the BDE-209 into plant, it was also closely related to the distribution of fatty acids in rice, altering BDE-209 accumulation in the rice. To safely use the iron mineral Fenton-like oxidation in the agricultural soil remediation, the safety of plant cells treated by mineral Fenton-like oxidation was evaluated using the transmission electron microscopy (TEM) and enzyme activity determination, which indicated that iron mineral Fenton-like oxidation would destroy the inner structures of plant cells, especially for G + 3H group.


Asunto(s)
Oryza , Contaminantes del Suelo , Ácidos Grasos , Éteres Difenilos Halogenados/análisis , Peróxido de Hidrógeno , Hierro/análisis , Minerales , Raíces de Plantas/química , Suelo , Contaminantes del Suelo/análisis
7.
J Environ Manage ; 287: 112309, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33730675

RESUMEN

Leachate generated in landfills are characterized by high toxicity related to, high concentrations of organic and non-organic pollutants, therefore, it is advisable to use highly effective methods of their purification to prevent environmental contamination. The aim of this study was to determine the treatment efficiency of landfill leachate during the application of integrated processes: advanced oxidation (AOP) and biochar adsorption (BC). In the case of the AOP, the chemical process was the Fenton reaction in which the source of hydroxyl radicals was H2O2 (system I) and SPC - sodium percarbonate (system II). Leachate was derived from the regional municipal waste landfill in Silesian Province (Poland) which was characterized by the pH of 8.40, COD value was 3155 mg L-1 and BOD was under 165 mg L-1. The BOD/COD ratio was at a low level (0.053) that indicated low biodegradability. The applied systems allowed of the COD reduction index to the level of 549 mg L-1 (H2O2+BC) and 944 mg L-1 (SPC+BC) where the limit threshold is 125 mg L-1. In the same case of the TOC index, these values were respectively 119 and 186 mg L-1, where the limit value is 30 mg L-1.This study has been aimed to estimate the toxicity of leachate treatment in integrated systems: AOP processes + biochar adsorption. Toxicity of treated and untreated leachate has been analysed by Sinapis alba and Lepidium sativum plants-based tests. It was found that while conducting the Fenton process as system I (H2O2) at all reagent doses, the removal efficiency of COD and TOC was 17% and 12% higher, respectively, compared to system II. The most advantageous dose of regents was 2.5:1 and biochar - 2 g L-1. It was observed that in the SPC+BC system the roots of L. sativum and S. alba were on average 34% and 28% longer compared to the H2O2+BC system. Higher germination index (GI) of both tested plants, indicating lower wastewater toxicity, was recorded for the SPC+BC system.


Asunto(s)
Contaminantes Químicos del Agua , Adsorción , Carbón Orgánico , Peróxido de Hidrógeno , Hierro , Oxidación-Reducción , Polonia , Contaminantes Químicos del Agua/análisis
8.
Water Sci Technol ; 83(5): 1217-1229, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33724948

RESUMEN

The ultraviolet photochemical degradation process is widely applied in wastewater treatment due to its low cost, high efficiency and sustainability. In this study, a novel rotating flow reactor was developed for UV-initiated photochemical reactions. The reactor was run in a continuous flow mode, and the tangential installation of the inlet and outlet on the annular reactor improved reaction rates. Numerical modelling, which combined solute transport, radiation transfer and photochemical kinetic degradation processes, was conducted to evaluate improvement compared to current reactor designs. Methylene Blue (MB) decomposition efficiency from the modelling results and the experimental data agreed well with each other. The model results showed that a rotational motion of fluid was well developed inside the designed reactor for a wide range of inflow rates; the generation of ·OH radicals significantly depended on UV irradiation dose, and thus the degradation ratio of MB showed a strong correlation with the UV irradiation distribution. In addition, the comprehensive numerical modelling showed promising potential for the simulation of UV/H2O2 processes in rotating flow reactors.


Asunto(s)
Peróxido de Hidrógeno , Purificación del Agua , Cinética , Procesos Fotoquímicos , Rayos Ultravioleta
9.
Water Sci Technol ; 83(5): 1242-1249, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33724950

RESUMEN

Recently, the utilization of wastes, recovery of high value-added products from waste, and their use as raw materials in other industries with the logic of industrial symbiosis has become an important issue. In this study, removal efficiency of Reactive Orange 16 (RO16) dye from aqueous solution was studied using TiO2 catalyst recycled from an industrial waste effluent. The recycling of TiO2 waste from a paints manufacturing industry was carried out by sintering the TiO2-containing waste. The catalyst usability of TiO2-containing powder was then investigated in the removal of RO16 dye by photocatalytic oxidation process. In photocatalytic oxidation studies, TiO2/UV and TiO2/UV/H2O2 processes were investigated, where the effect of annealing temperature, the effect of H2O2 concentration, and the adsorption and photocatalytic effects on RO16 dye removal were studied. The results showed that 100% RO16 removal was obtained for the 10 mg/L initial RO16 concentration using the TiO2 (1 g/L)/UV process. At high initial RO16 concentration (50 mg/L), 100% RO16 removal was achieved by adding 10 mM H2O2.


Asunto(s)
Peróxido de Hidrógeno , Purificación del Agua , Catálisis , Titanio , Aguas Residuales
10.
J Vis Exp ; (168)2021 02 09.
Artículo en Inglés | MEDLINE | ID: mdl-33645566

RESUMEN

Reactive oxygen species (ROS) are well-established signaling molecules, which are important in normal development, homeostasis, and physiology. Among the different ROS, hydrogen peroxide (H2O2) is best characterized with respect to roles in cellular signaling. H2O2 has been implicated during the development in several species. For example, a transient increase in H2O2 has been detected in zebrafish embryos during the first days following fertilization. Furthermore, depleting an important cellular H2O2 source, NADPH oxidase (NOX), impairs nervous system development such as the differentiation, axonal growth, and guidance of retinal ganglion cells (RGCs) both in vivo and in vitro. Here, we describe a method for imaging intracellular H2O2 levels in cultured zebrafish neurons and whole larvae during development using the genetically encoded H2O2-specific biosensor, roGFP2-Orp1. This probe can be transiently or stably expressed in zebrafish larvae. Furthermore, the ratiometric readout diminishes the probability of detecting artifacts due to differential gene expression or volume effects. First, we demonstrate how to isolate and culture RGCs derived from zebrafish embryos that transiently express roGFP2-Orp1. Then, we use whole larvae to monitor H2O2 levels at the tissue level. The sensor has been validated by the addition of H2O2. Additionally, this methodology could be used to measure H2O2 levels in specific cell types and tissues by generating transgenic animals with tissue-specific biosensor expression. As zebrafish facilitate genetic and developmental manipulations, the approach demonstrated here could serve as a pipeline to test the role of H2O2 during neuronal and general embryonic development in vertebrates.


Asunto(s)
Técnicas Biosensibles/métodos , Peróxido de Hidrógeno/metabolismo , Imagen Molecular/métodos , Neurogénesis , Especies Reactivas de Oxígeno/metabolismo , Células Ganglionares de la Retina/metabolismo , Pez Cebra/metabolismo , Animales , Células Cultivadas , Peróxido de Hidrógeno/análisis , Oxidación-Reducción , Células Ganglionares de la Retina/citología , Pez Cebra/crecimiento & desarrollo
11.
J Environ Manage ; 285: 112155, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33652186

RESUMEN

Fenton reaction is an oxidation process of interest in wastewater treatment because of its ability to degrade organic compounds. Iron-based magnetic particles can be a very useful catalyst when using heterogeneous Fenton process. The major problem of this heterogeneous process is the saturation of the Fe 3+ on the surface, which limits the process. In this study, the possibility of using magnetite particles as a substrate is presented, increasing its degradation efficiency by Fenton reaction through a regeneration process that achieves the electronic reduction of its surface using reducing agents. The results indicate that the regeneration process is quite effective, increasing the efficiency of the degradation of Methylene Blue up to 99%. The concentration of magnetite is the most influential factor in the efficiency of the reaction, while the regeneration time and the concentration of reducing agent do not significantly affect the results considering the range used. The presence of mechanical stirring may adversely affect the reaction in the long term. Increasing the oxidant agent concentration reduces the initial speed of the reaction but not the long-term efficiency. The use of hydrazine in this process allows the successive reuse of these particles maintaining a high percentage of elimination of methylene blue, above 70% even after 10 uses, compared to an elimination below 20% for particles not regenerated after the second use and for particles regenerated with ascorbic acid after the eighth use.


Asunto(s)
Contaminantes Químicos del Agua , Purificación del Agua , Catálisis , Peróxido de Hidrógeno , Fenómenos Magnéticos , Oxidación-Reducción , Agua , Contaminantes Químicos del Agua/análisis
12.
Nat Commun ; 12(1): 1415, 2021 03 03.
Artículo en Inglés | MEDLINE | ID: mdl-33658510

RESUMEN

Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after H2O2 treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Cisteína/metabolismo , Animales , Antioxidantes/metabolismo , Caenorhabditis elegans/efectos de los fármacos , Caenorhabditis elegans/microbiología , Proteínas de Caenorhabditis elegans/genética , Peróxido de Hidrógeno/farmacología , MAP Quinasa Quinasa 4/metabolismo , Proteínas Quinasas Activadas por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Mutación , Oxidación-Reducción , Proteómica/métodos , Transducción de Señal , Factores de Transcripción/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo
13.
Water Res ; 195: 116956, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33676178

RESUMEN

Due to the hydrophobicity and large specific surface area microplastics (MPs) have become the vector for the migration of environmental organic pollutants. Environmental aging process affects the physiochemical structure of MPs and their corresponding environmental behaviors, in which the effect of bisphenol A (BPA) binding mode within plastic matrix on aging behaviors of MPs is not reported. In this work, the structural properties and BPA sorption behaviors of low density polyethylene (LDPE) MPs with BPA additives and polycarbonate (PC) MPs with BPA monomers exposed to three types of artificial accelerated aging processes including UV/H2O, UV/H2O2, and UV/Cl2 systems were comparatively investigated. Virgin LDPE and PC exhibited obvious leakage of BPA additives or monomers. Aged LDPE had stronger sorption ability towards BPA in water environment with no observed leakage of BPA additives. While, aged PC had extremely high leakage of BPA monomers, which is similar to virgin PCs and was proved to be a persistent source of BPA release. The BPA sorption on aged LDPE or leaching from aged PC was influenced by aging processes, water pH, salinity, co-existing estradiol (E2), and water sources. This study reveals the potential ecological and environmental risks of MPs containing toxic additives/monomers during aging processes from a new perspective.


Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Adsorción , Compuestos de Bencidrilo , Peróxido de Hidrógeno , Fenoles , Plásticos , Agua , Contaminantes Químicos del Agua/análisis
14.
Water Res ; 195: 116988, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33714011

RESUMEN

Microbial dissimilatory iron reduction and aerobic oxidation affect the biogeochemical cycles of many elements. Although the processes have been widely studied, the underlying mechanisms, and especially how the surface structures of iron oxides affect these redox processes, are poorly understood. Therefore, {001} facet-dominated hematite nanoplates (HNP) and {100} facet-dominated hematite nanorods (HNR) were used to explore the effects of surface structure on the microbial dissimilatory iron reduction and aerobic oxidation processes. During the reduction stage, the production of total Fe(II) normalized by specific surface area (SSA) was higher for HNP than HNR due to steric effects and the ligand-bound conformation of the connection between iron on different exposed facets and electron donors from microorganisms. However, during the aerobic oxidation stage, both the SSA- and Fe(II)-normalized reactive oxygen species (ROS), including hydrogen peroxide (H2O2) and hydroxyl radical (•OH), were higher for HNR than HNP. Theoretical calculation results showed that the {100} facets exhibited a lower activation energy barrier for oxygen reduction reaction than {001} facets, supporting the experimental observation that {100} facet-dominated HNR had a higher ROS production efficiency than {001} facet-dominated HNP. These results indicated that surface characteristics not only mediated the microbial reduction of Fe(III) but also affected the aerobic oxidation of microbially reduced Fe(II). Accessibility of electron donors to surface iron atom determined the reduction of Fe(III), and activation energy barrier for oxygen reduction by surface Fe(II) dominated the ROS production during the redox processes. This study advances our understanding of the mechanisms through which ROS are produced by iron (oxyhydr)oxides during microbial dissimilatory iron reduction and aerobic oxidation processes.


Asunto(s)
Compuestos Férricos , Shewanella , Peróxido de Hidrógeno , Hierro , Oxidación-Reducción , Especies Reactivas de Oxígeno
15.
Carbohydr Polym ; 260: 117796, 2021 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-33712144

RESUMEN

The polysaccharide (DRP) was gained from dandelion roots by ultrasonic-assisted enzymatic extraction (UAEE) followed by two-step column purification. Then selenylation of DRP has been accomplished by HNO3-Na2SeO3 method. sDRP-1 and sDRP-2 with the selenium content of 170 ± 1.13 and 710 ± 4.00 µg/g were prepared for further structural characterization and bioactivity determination. DRP, sDRP-1, and sDRP-2 were composed of the same monosaccharides in different molar ratios, and the molecular weights of DRP, sDRP-1 and sDRP-2 were 8700, 7900, and 5600 Da, respectively. Fourier transform infrared (FT-IR) spectra confirmed that DRP, sDRP-1, and sDRP-2 possessed similar functional groups. The results of Congo red test, X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed that DRP, sDRP-1, and sDRP-2 had no three helix structure, did not form single crystal, and all belonged to amorphous morphology. sDRP-1 and sDRP-2 possessed greater antioxidant activities in vitro than the native polysaccharide DRP. At the same time, the selenized polysaccharides showed better immunomodulatory ability and could be used as new-type immunoenhancer. The present conclusions provided theoretical basis for the new application of dandelion polysaccharides and the development of dandelion resources.


Asunto(s)
Antioxidantes/química , Polisacáridos/química , Selenio/química , Taraxacum/metabolismo , Animales , Supervivencia Celular/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Factores Inmunológicos/química , Factores Inmunológicos/metabolismo , Factores Inmunológicos/farmacología , Macrófagos/citología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Ratones , Peso Molecular , Fagocitosis/efectos de los fármacos , Raíces de Plantas/metabolismo , Polisacáridos/metabolismo , Polisacáridos/farmacología , Células RAW 264.7
16.
Food Chem ; 352: 129458, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33714166

RESUMEN

We investigated the effect of exogenous glutathione (GSH) on chilling injury (CI) in postharvest bell pepper fruits stored at low temperature and explored the mechanism of this treatment from the perspective of the ascorbate-glutathione (AsA-GSH) cycle. Compared with the control, fruits treated with exogenous GSH before refrigeration displayed only slight CI symptoms and mitigated CI-induced cell damage after 10 d. Moreover, the treated peppers had lower lipid peroxidation product, H2O2, and O2- content than those did the control. Glutathione treatment enhanced the ascorbate-glutathione cycle by upregulating CaAPX1, CaGR2, CaMDHAR1, and CaDHAR1 and the antioxidant enzymes APX, GR, and MDHAR associated with the ascorbate-glutathione cycle. Glutathione treatment also increased ascorbate and glutathione concentrations. Taken together, our results showed that exogenous GSH treatment could alleviate CI in pepper fruits during cold storage by triggering the AsA-GSH cycle and improving antioxidant capacity.


Asunto(s)
Ácido Ascórbico/metabolismo , Capsicum/efectos de los fármacos , Capsicum/metabolismo , Frío , Glutatión/farmacología , Frutas/efectos de los fármacos , Frutas/metabolismo , Glutatión/metabolismo , Peróxido de Hidrógeno/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Plantones/efectos de los fármacos , Plantones/metabolismo
17.
Rev Gaucha Enferm ; 42(spe): e20200146, 2021.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-33787720

RESUMEN

OBJECTIVE: To evaluate the protocols on decontamination/reuse of N95 masks available in the literature in times of the Covid-19 pandemic. METHOD: Integrative literature review, in the period from 2010 to 2020, on the databases MEDLINE/PubMed, Science Direct, Cochrane, SAGE journals, Web of Science, Scopus, Embase and Wiley, with the descriptors Masks AND Respiratory protective devices; Mask OR N95 AND Covid-19; N95 AND Respirators; Decontamination AND N95 AND Coronavirus; Facemask OR Pandemic. RESULTS: Twelve studies were included, of which 3 (30.0%) used ultraviolet germicidal irradiation and indicated mask deterioration between 2 and 10 cycles, 4 (40.0%) used hydrogen peroxide vapor, and seal loss varied from 5 to 20 cycles, 4 (33.3%) evaluated the structural integrity of the N95 mask through visual inspection and 6 (54.4%), its filtration efficiency. CONCLUSION: Reuse strategies to overcome a shortage of devices in the face of the pandemic challenge the current concept for good practices in health-product processing.


Asunto(s)
/epidemiología , Descontaminación/métodos , Equipo Reutilizado , Pandemias , Antiinfecciosos Locales , Desinfectantes , Desinfección/métodos , Óxido de Etileno , Filtración/instrumentación , Humanos , Peróxido de Hidrógeno , Rayos Ultravioleta
18.
Molecules ; 26(4)2021 Feb 18.
Artículo en Inglés | MEDLINE | ID: mdl-33670455

RESUMEN

Many studies suggest anthocyanins may prevent the development of several diseases. However, anthocyanin bioactivity against cellular stress is not fully understood. This study aimed to evaluate the protective effect of berry anthocyanins on stressed cells using Saccharomyces cerevisiae. The impact of in vitro gastrointestinal digestion on anthocyanin profiles was also assessed. Bilberry and blackcurrant had higher anthocyanin levels than raspberry and strawberry, but digestion reduced the detected anthocyanins by approximately 90%. Yeast cells with and without digested or nondigested anthocyanin extracts were exposed to H2O2 and examined for survival. In the presence of anthocyanins, particularly from digested strawberry, a significant increase in cell survival was observed, suggesting that the type and levels of anthocyanins are important factors, but they also need to undergo gastrointestinal (GI) structural modifications to induce cell defence. Results also showed that cells need to be exposed to anthocyanins before the stress was applied, suggesting induction of a cellular defence system by anthocyanins or their derivatives rather than by a direct antioxidative effect on H2O2. Overall, data showed that exposure of severely stressed yeast cells to digested berry extracts improved cell survival. The findings also showed the importance of considering gastrointestinal digestion when evaluating anthocyanins' biological activity.


Asunto(s)
Frutas/química , Peróxido de Hidrógeno/toxicidad , Viabilidad Microbiana/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Saccharomyces cerevisiae/citología , Antocianinas/análisis , Antocianinas/química , Sustancias Protectoras/farmacología , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/crecimiento & desarrollo
19.
Molecules ; 26(4)2021 Feb 18.
Artículo en Inglés | MEDLINE | ID: mdl-33670593

RESUMEN

Information concerning the mechanisms underlying oxidative stress and low-grade inflammation in young healthy women predisposing eventually to future diseases is scarce. We investigated the relationship of oxidative stress and high-sensitivity C-reactive protein (hsCRP) in fertile-age women by oral combined contraceptive (OC) use. Caucasian Italian healthy non-obese women (n = 290; 100 OC-users; 190 non-OC-users; mean age 23.2 ± 4.7 years) were analyzed. Blood hydroperoxides, as oxidative stress biomarkers, were assessed by Free Oxygen Radical Test (FORT). Serum hsCRP was determined by an ultra-sensitive method (hsCRP). Markedly elevated oxidative stress (≥400 FORT Units) was found in 77.0% of OC-users and 1.6% of non-OC-users, odds ratio (OR) = 209, 95% CI = 60.9-715.4, p < 0.001. Elevated hsCRP levels ≥ 2.0 mg/L, considered risky for cardiovascular diseases (CVDs), were found in 41.0% of OC-users and 9.5% of non-OC-users, OR = 6.6, 95%CI 3.5-12.4, p < 0.001. Hydroperoxides were strongly positively correlated to hsCRP in all women (rs = 0.622, p < 0.001), in OC-users (rs = 0.442, p < 0.001), and in non-OC-users (rs = 0.426, p < 0.001). Women with hydroperoxides ≥ 400 FORT Units were eight times as likely to have hsCRP ≥ 2 mg/L. In non-OC-users only, hydroperoxides values were positively correlated with weight and body mass index, but negatively correlated with red meat, fish and chocolate consumption. Our research is the first finding a strong positive correlation of serum hydroperoxides with hsCRP, a marker of low-grade chronic inflammation, in young healthy women. Further research is needed to elucidate the potential role of these two biomarkers in OC-use associated side-effects, like thromboembolism and other CVDs.


Asunto(s)
Proteína C-Reactiva/metabolismo , Anticonceptivos Orales Combinados/farmacología , Estrés Oxidativo , Adolescente , Adulto , Biomarcadores/metabolismo , Humanos , Peróxido de Hidrógeno/metabolismo , Persona de Mediana Edad , Factores de Riesgo , Adulto Joven
20.
Molecules ; 26(4)2021 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-33673263

RESUMEN

The current research is focused on studying the biological efficacy of flexirubin, a pigment extracted from Chryseobacterium artocarpi CECT 8497.Different methods such as DPPH, H2O2, NO•, O2•-, •OH, lipid peroxidation inhibition by FTC and TBA, ferric reducing and ferrous chelating activity were carried out to evaluate the antioxidant activity of flexirubin. Molecular docking was also carried out, seeking the molecular interactions of flexirubin and a standard antioxidant compound with SOD enzyme to figure out the possible flexirubin activity mechanism. The new findings revealed that the highest level of flexirubin exhibited similar antioxidant activity as that of the standard compound according to the H2O2, •OH, O2•-, FTC and TBA methods. On the other hand, flexirubin at the highest level has shown lower antioxidant activity than the positive control according to the DPPH and NO• and even much lower when measured by the FRAP method. Molecular docking showed that the interaction of flexirubin was in the binding cavity of the SOD enzyme and did not affect its metal-binding site. These results revealed that flexirubin has antioxidant properties and can be a useful therapeutic compound in preventing or treating free radical-related diseases.


Asunto(s)
Antioxidantes/química , Pigmentos Biológicos/química , Polienos/química , Superóxido Dismutasa/química , Compuestos de Bifenilo/química , Chryseobacterium/química , Peróxido de Hidrógeno/química , Simulación del Acoplamiento Molecular , Óxido Nítrico/química , Picratos/química , Pigmentación/efectos de los fármacos
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