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1.
Braz. j. oral sci ; 20: e210219, jan.-dez. 2021. ilus
Artículo en Inglés | LILACS, BBO - Odontología | ID: biblio-1253954

RESUMEN

Aim: This study evaluated the chemical composition of Lippia thymoides (Lt) essential oil and its antimicrobial activity against fungal strains of Candida albicans (Ca) and Gram-negative bacteria Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn). Methods: Lt essential oil was obtained by hydrodistillation apparatus with a modified Clevenger extension. The chemical analysis was analyzed by gas phase chromatography and mass spectrometry on Shimadzu QP 2010 plus. Sample sensitivity evaluation was performed by ABHb-inoculum and culture plates were developed with triphenyltetrazolium chloride, also Fn and Pi samples analysis were in anaerobic environment and Ca sample analysis was performed in aerobic environment. The minimum inhibitory concentration (CIM) was determinated by microdilution in eppendorfs tubes. Results: The chemical analysis showed that Thymol (59,91%) is the main compound found in Lt essential oil, also other antifungal and antimicrobial agents were present γ-terpinene (8.16%), p-cymene (7.29%) and ß-caryophyllene (4.49%), Thymol is a central ingredient of many medicinal plants and has a potent fungicidal, bactericidal and antioxidant activity, it has been previously shown to have anti-inflammatory activity against Periodontal Disease (PD) cause can reduces prostanoids, interleukins, leukotrienes levels in periodontium. CIM result Pi was 6.5 µg/mL, Fn was 1.5 µg/mL and Ca was 0.19 µg/mL. Conclusion: The antimicrobial activity of L. thymoides, through the compound Thymol, has been shown promising potential against gram-negative periodontopathogenic bacteria and fungi whose therapeutic arsenal is still very restricted


Asunto(s)
Periodontitis , Aceites Volátiles , Extractos Vegetales , Lippia , Microbiota , Antifúngicos
2.
Rev. Odontol. Araçatuba (Impr.) ; 42(3): 50-55, set.-dez. 2021.
Artículo en Portugués | LILACS, BBO - Odontología | ID: biblio-1291673

RESUMEN

A pandemia gerada pelo SARS-CoV-2 gerou uma série de estudos sobre essa nova doença. Este cenário proporcionou mudanças na odontologia, modificando a forma de atendimento e a rotina do consultório. O conhecimento sobre as implicações desse vírus no sistema estomatognático pode fornecer informações valiosas no conhecimento sobre o mecanismo dessa doença na cavidade oral. Dessa forma, foi realizada uma revisão de literatura, a fim de discutir os efeitos do COVID-19 na cavidade oral. No entanto, há poucas evidências na literatura sobre a correlação da COVID-19 e manifestações orais. Pode-se concluir que o vírus possui reflexo na cavidade oral, apresentando diferentes alterações, tais como: úlceras, periodontite, alterações do paladar, infecções oportunistas, dentre outras. Ainda se faz necessária a realização de mais estudos, para compreensão dos mecanismos fisiopatológicos da doença. Além disso, o trabalho ressalta a importância do cirurgião-dentista no ambiente hospitalar, para diagnóstico e conduta no tratamento dessas manifestações na cavidade oral(AU)


The SARS-CoV-2 pandemic has spawned several studies on this new disease. This scenario provided changes in Dentistry by modifying the form of care and the routine of the office. The knowledge about the implications of this virus in the stomatognathic system can provide valuable information in the knowledge about the mechanism of this disease in the oral cavity. Thus, a literature review was carried out to discuss the effects of COVID-19 on the oral cavity. However, there is little evidence in the literature about the correlation between COVID-19 and oral manifestations. It can be concluded that the virus is reflected in the oral cavity, such as ulcers, periodontitis, changes in taste, opportunistic infections, among others. Further studies are still needed to understand the pathophysiological mechanisms of the disease. Besides, the work highlights the importance of the Dental Surgeon in the hospital environment for diagnosis and management in the treatment of these manifestations in the oral cavity(AU)


Asunto(s)
Manifestaciones Bucales , Atención Odontológica , COVID-19 , Periodontitis , Úlcera , Infecciones Oportunistas , Sistema Estomatognático , Odontólogos , Boca
3.
Braz Dent J ; 32(2): 27-36, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34614058

RESUMEN

The objective of this 9-month clinical study is to assess the impact of one-stage full-mouth disinfection (FMD) on salivary nitrite levels and systemic biomarkers and its correlation with total subgingival bacterial load in obese and non-obese patients with periodontitis. In total, 94 patients (55 obese and 39 non-obese) were initially evaluated, seven were lost during follow-up, resulting in 87 individuals at the end of the study. Outcomes were assessed at baseline, 3, 6, and 9 months post periodontal treatment by FMD. Salivary nitrite levels were determined using Griess reagent. Blood samples were collected to determine C-Reactive Protein (CRP), alkaline phosphatase and fasting blood glucose. Real-time PCR was used to determine the total subgingival bacterial load. FMD protocol resulted in increased salivary nitrite levels at 6- and 9-months post-treatment in the non-obese group (p<0.05). In obese individuals, FMD treatment led to an increase in salivary nitrite levels at 6 months (p<0.05); however, at 9 months, the nitrite levels returned to baseline levels. For both groups, the highest nitrite values were observed at 6 months. In addition, in both groups, FMD was associated with a decrease in biomarkers related to systemic inflammation and cardiovascular diseases, such as CRP (p<0.05) and alkaline phosphatase (p<0.05), and had no impact on the fasting blood glucose. This study demonstrates that obese patients with periodontitis present similar salivary nitrite levels when compared with non-obese individuals. FMD protocol resulted in increases in salivary nitrite levels and was associated with a positive impact on systemic biomarkers, regardless of obesity status.


Asunto(s)
Nitritos , Periodontitis , Biomarcadores , Desinfección , Humanos , Obesidad/complicaciones
4.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(10): 992-997, 2021 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-34619893

RESUMEN

Objective: To evaluate the effect of extraction treatment on orthodontic patients with Stage Ⅳ/Grade C periodontitis. Methods: Eight orthodontic patients with Stage Ⅳ/Grade C periodontitis (3 males, 5 females, 25~38 years old) who had finished extraction treatment during January 2003 and December 2013 were included in the study. The patients accepted periodontal treatment and extraction orthodontic treatment in the Department of Periodontology and Department of Orthodontics at Peking University School and Hospital of Stomatology. Clinical examination records and periapical films of 34 teeth adjacent to extraction sites (TAES) and 34 teeth non-adjacent to extraction sites (TNES) were evaluated and compared. Probing depth (PD) and relative bone height (RBH) before and after orthodontic treatment were also compared. Results: No significant difference was found between PD of TAES and that of TNES (P>0.05). After orthodontic treatment, RBH of TAES was increased by 8.19% (-3.36%,14.01%) (P<0.05). RBH of TAES far from extraction sites was elevated by 7.73% (-1.52%, 21.55%)(P<0.05). Tooth resorption rate of TAES was 13.1% (1.3%, 23.9%)and TNES was 4.3% (-8.19%, 12.5%) after orthodontic treatment, and the difference was statistically significant. Conclusions: Under proper combined periodontal and orthodontic treatment, stability of periodontal status in patients with Stage Ⅳ/Grade C periodontitis can be maintained. Relative bone height of extraction sites can be elevated after orthodontic treatment.


Asunto(s)
Periodontitis , Diente , Adulto , Atención Odontológica , Femenino , Humanos , Masculino , Periodoncia , Periodontitis/terapia
5.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(10): 1011-1019, 2021 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-34619896

RESUMEN

Objective: To investigate the effects of combination treatment of photodynamic therapy (PDT) based on photosensitizer chlorin e6 (Ce6) and antibiotic agent tinidazole (TNZ) against periodontitis both in vitro and in vivo. Methods: The Sprague-Dewley (SD) rat periodontitis model was constructed using the method of orthodontic wire ligation. After successful modeling, SD rats were randomly divided into the following 6 groups (3 rats in each group): positive control (Ctrl+), Ce6, TNZ, a mixture of Ce6 and TNZ (Ce6/TNZ), Ce6 with laser irradiation (Ce6+L), a mixture of Ce6 and TNZ with laser irradiation (Ce6/TNZ+L). Methyl thiazolyl tetrazolium (MTT) assay was used to assess the cytotoxic activities of Ce6 (concentration range: 0-20 mg/L), TNZ (concentration range: 0-16.6 mg/L) and their mixture (Ce6/TNZ) in mouse fibroblast L929 cells. Fluorescence probe method was applied to measure the production of reactive oxygen species in the dental plaque biofilms after various treatments with and without 5-minute laser irradiation at 635 nm at a power density of 0.5 W/cm2 (Ce6+L and Ce6/TNZ+L groups), thus to evaluate the PDT performances. Cell counting kit-8 (CCK-8) and live/dead staining were used to assess the antibacterial activity in each of the groups and the combination index (CI) of PDT combined with TNZ was calculated subsequently. Flow cytometry was utilized to detect the apoptosis-inducing effects of these treatments in macrophage RAW264.7 cells after processing with the apoptosis detection kit. The inhibitory effects of various treatments on the absorption of alveolar bone of SD rats were further evaluated in the periodontitis rats by using the micro-CT. Results: The survival rates of L929 cells in the preset concentration range were all above 90% in Ce6, TNZ and Ce6/TNZ groups. Upon laser irradiation, the plaque biofilms in Ce6 and Ce6/TNZ groups showed significant green fluorescence, indicating that large amounts of reactive oxygen species were triggered and generated significantly in the dental plaque biofilms. However, the survival rates of dental plaque microorganisms in 5 Ce6/TNZ concentrations were (85.4±5.5)%, (76.0±8.9)%, (61.7±0.6)%, (56.3±2.6)% and (43.5±0.6)% respectively, which were significantly lower than that in Ce6 only and TNZ only groups (P<0.01). The CI levle of each drug concentration group was less than 1.0, which showed a significant synergistic antibacterial efficiency. Stronger apoptotic activities were observed in Ce6+L and Ce6/TNZ+L groups compared with those in Ce6 only and Ce6/TNZ only groups (P<0.01). In periodontitis rats, Ce6/TNZ combined laser irradiation could effectively inhibit the absorption of alveolar bone. The alveolar bone volume and the ratio of bone volume and tissue volume were (1.49±0.07) mm3 and (47.08±0.71)%, respectively. The distances between cementoenamel junction to alveolar bone crest on buccal and palatal sites decreased to (2.13±0.07) mm and (1.94±0.10) mm respectively, showing a high inhibition efficiency. Conclusions: Ce6-mediated PDT combined with TNZ possessed notable synergistic effects against periodontitis, reflecting in the efficient antibacterial effect, the apoptosis-inducing action on macrophages, and the inhibitory efficacy on the alveolar bone absorption in vivo.


Asunto(s)
Antineoplásicos , Periodontitis , Fotoquimioterapia , Animales , Antibacterianos , Ratones , Periodontitis/tratamiento farmacológico , Fármacos Fotosensibilizantes/uso terapéutico , Ratas
7.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(10): 1034-1039, 2021 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-34619900

RESUMEN

Many new microbial species which are correlated with periodontitis have been identified in addition to the"red complex"cultured by conventional methods due to the development of high-throughput sequencing technology. Filifactor alocis (Fa), as a anaerobic Gram-positive bacteria, is closely related to the occurrence and development of periodontitis. Fa can not only destroy host cells and induce immune escape response, but also interact with other periodontal pathogens to promote the periodontitis. This article summarizes and discusses the research progress on the relationship between Fa and periodontitis in recent years and highlighs a new way to investigate the etiology, prevention and treatment of periodontitis.


Asunto(s)
Periodontitis , Clostridiales , Humanos
8.
BMC Oral Health ; 21(1): 471, 2021 09 25.
Artículo en Inglés | MEDLINE | ID: mdl-34563175

RESUMEN

BACKGROUND: Periodontitis is a chronic inflammatory disorder with a complex etiology. Long non-coding RNAs (lncRNAs) have been shown to affect pathoetiology of periodontitis. We aimed at identification of expression of five lncRNAs, namely Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 in the circulation and gingival tissues of these patients compared with healthy controls. METHODS: In a pilot case-control study, we compared expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 lncRNAs between blood and tissue samples of patients with periodontitis and healthy controls using real time quantitative PCR technique. The present work was performed on samples got from 26 patients with periodontitis and 28 controls. Female/male ratio was 16/10 and 12/16 in cases and controls, respectively. RESULTS: There was no significant difference in the expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 genes between affected and unaffected tissues. However, expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 genes were significantly lower in the blood samples of patients when compared with control samples (Ratio of mean expression = 0.16, 0.14, 0.13, 0.10 and 0.14, respectively). Subsequently, we compared expressions of these lncRNAs between patients and controls in a sex-based manner. Expressions of Linc00667, FENDRR and DIRC3 genes were significantly lower in female patients compared with female controls (RME = 0.09, 0.07 and 0.10, respectively). Yet, there was no significant difference in expression of any of mentioned lncRNAs among male subgroups. Consistent with the similar levels of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 in tissue samples of patients and controls, none of them could separate these two sets of samples. However, AUC values for of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 expression levels in blood samples were 0.66, 0.72, 0.70, 0.72, 0.70 and 0.68, respectively with FENDRR having the best sensitivity value. CONCLUSION: Taken together, lncRNAs might be involved in the pathologic events in the circulation of patients with periodontitis.


Asunto(s)
Periodontitis , ARN Largo no Codificante , Estudios de Casos y Controles , Femenino , Encía , Humanos , Masculino , Proyectos Piloto , ARN Largo no Codificante/genética
9.
BMC Oral Health ; 21(1): 479, 2021 09 27.
Artículo en Inglés | MEDLINE | ID: mdl-34579707

RESUMEN

BACKGROUND: Periodontitis can lead to the destruction of periodontium and adversely influence the overall health, wellbeing, and quality of life. However, studies on the relationship between severe periodontitis and serum 25-hydroxyvitamin D [25(OH)D] are limited. This study is designed to explore the relationship between 25(OH)D and severe periodontitis. METHODS: A cross-section study of 2928 participants enrolled from the National Health and Nutrition Examination Survey (NHANES) from 2013 to 2014 was conducted. The periodontal examination was performed using a total oral periodontal examination program, and probe measurements were collected at six sites per tooth in NHANES. Severe periodontitis was characterized as: ≥ 2 interproximal sites with attachment loss (AL) ≥ 6 mm (not on the same tooth) and ≥ 1 interproximal site with probing depth (PD) ≥ 5 mm. Severe periodontitis and serum 25(OH)D were the dependent and independent variables, respectively. Univariate, multivariate, and subgroup analyses were performed to explore the relationship between severe periodontitis and serum 25(OH)D. RESULTS: Among the 2928 participants, the average age of the population was 50 ± 13.71 years old, with 1425 (48.67%) males, 316 (10.79%) exhibited severe periodontitis. Serum 25(OH)D showed a significantly negative association with severe periodontitis after adjusting all variables (OR 0.75, 95% CI 0.63-0.89). In addition, severe periodontitis has a nonlinear relationship with serum 25(OH)D, whoes inflection point was 102 (nmol/L). On the left side of the inflection point (25(OH)D ≤ 102 nmol/L), the effect size was 0.98 and 95%CI was 0.98-0.99 (25(OH)D per 1 nmol/L increments). On the right side of the inflection point (25(OH)D > 102 nmol/L), the effect size was 0.99 and 95% CI was 0.98-1.01. The subgroup analysis showed pronounced changes in non-Hispanic white, alcohol consumption, diabetes, and health insurance. CONCLUSION: Serum 25 (OH) D in relation to severe periodontitis is nonlinear in our study.When serum 25 (OH) D is less than 102 nmol/L, serum 25 (OH) D is negatively associated with severe periodontitis.


Asunto(s)
Periodontitis , Calidad de Vida , Adulto , Estudios Transversales , Humanos , Masculino , Persona de Mediana Edad , Encuestas Nutricionales , Periodontitis/complicaciones , Periodontitis/epidemiología , Vitamina D/análogos & derivados
10.
J Appl Oral Sci ; 29: e20210160, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34586188

RESUMEN

OBJECTIVE: This study aims to evaluate the effect of ellagic acid (EA) by measuring the levels of alveolar bone resorption and inflammatory and oxidative stress markers in the periodontal tissues and serum on the periodontal repair process related to experimental periodontitis in rats. METHODOLOGY: Forty Wistar rats were divided into four study groups as follows: Group 1=healthy control (n=10); Group 2=EA control (15 mg/kg)(n=10); Group 3=periodontitis (n=10); Group 4=periodontitis+EA (15 mg/kg) (n=10). The periodontitis model was established by ligating bilateral mandibular first molars for 14 days. Then, rats were given normal saline or EA for another 14 days by gavage administration. Serum and gingiva myeloperoxidase (MPO) activity, 8-hydroxydeoxyguanosine(8-OHdG), and glutathione (GSH) levels were analyzed by ELISA. Immunohistochemical analysis was used to detect Interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) immunoreactivities in the periodontal tissues. Alveolar bone loss (ABL) and attachment loss (AL) was evaluated by histomorphometry analysis. RESULTS: ABL and AL were statistically higher in group 3 than in groups 1, 2 and 4 and in group 4 than in groups 1 and 2 (p<0.05). MPO activities in gingival tissue and serum were significantly increased in group 3 compared to groups 1 and 2 (p<0.05). Significantly higher serum GSH levels, lower gingiva, and serum 8-OHdG levels, and MPO activity were observed in group 4 compared to group 3 (p<0.05). Rats with periodontitis (group 3) expressed significantly higher immunoreactivities of IL-6 and TNF-α and lower IL-10 immunoreactivity compared to those other groups (p<0.05). IL-6 and TNF-α immunoreactivities significantly decreased and IL-10 immunoreactivity increased in group 4 after the use of EA compared to group 3 (p<0.001). CONCLUSIONS: Our findings showed that EA provides significant improvements on gingival oxidative stress and inflammatory markers and alveolar bone resorption in the repair process associated with experimental periodontitis. Therefore, EA may have a therapeutic potential on periodontitis.


Asunto(s)
Pérdida de Hueso Alveolar , Periodontitis , Animales , Ácido Elágico/farmacología , Interleucina-1beta , Periodontitis/tratamiento farmacológico , Ratas , Ratas Wistar , Factor de Necrosis Tumoral alfa
11.
Braz Oral Res ; 35(Supp 2): e095, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34586209

RESUMEN

The aim of this review was to update the evidence of new approaches to non-surgical therapy (NSPT) in the treatment of periodontitis. Preclinical and clinical studies addressing the benefits of adjunctive antimicrobial photodynamic therapy, probiotics, prebiotics/synbiotics, statins, pro-resolving mediators, omega-6 and -3, ozone, and epigenetic therapy were scrutinized and discussed. Currently, the outcomes of these nine new approaches, when compared with subgingival debridement alone, did not demonstrate a significant added clinical benefit. However, some of these new alternative interventions may have the potential to improve the outcomes of NSPT alone. Future evidence based on randomized controlled clinical trials would help clinicians and patients in the selection of different adjunctive therapies.


Asunto(s)
Antiinfecciosos , Periodontitis , Probióticos , Antibacterianos/uso terapéutico , Raspado Dental , Humanos , Periodontitis/tratamiento farmacológico
12.
Braz Oral Res ; 35(Supp 2): e099, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34586213

RESUMEN

There are several studies that evaluate the use of lasers in periodontal treatment in non-surgical or surgical therapy. However, while several studies showed clinically beneficial effects of some lasers in periodontal treatment, there are few clinical reports of additional advantages of lasers as adjunctive treatments in periodontology. The aim of this paper is to demonstrate and critically analyze the level of scientific evidence of effects of low-level lasers and high-power lasers in periodontology. A narrative review of the studies was carried out in each topic and type of laser or periodontal treatment. In nonsurgical periodontal therapy the results showed that there is an additional clinical benefit when using a diode laser (DL) associated with scaling and root planing (SRP) in patients with moderate to severe periodontitis. The Er:YAG laser seems to be the most suitable for nonsurgical periodontal therapy and promotes the same clinical effects as conventional therapy. In periodontal surgery vaporization of the gingival or mucosal tissue can be carried out with DL, CO2, Nd:YAG, Er:YAG and Er,Cr:YSGG lasers. Photobiomodulation (PBM), mediated by low-level lasers associated with non-surgical periodontal therapy, promotes additional benefits in the short term and accelerates the bone and gingival tissue repair process and also reduces postoperative symptoms of periodontal surgery. The effect of antimicrobial Photodynamic Therapy is relevant in the initial reevaluation periods. Studies have shown controversial results of the use of lasers in periodontics, and this fact may be due to the lack of standard parameters of irradiation in each clinical application.


Asunto(s)
Periodontitis Crónica , Terapia por Láser , Láseres de Estado Sólido , Periodontitis , Raspado Dental , Humanos , Láseres de Semiconductores/uso terapéutico , Láseres de Estado Sólido/uso terapéutico , Aplanamiento de la Raíz
13.
Braz Oral Res ; 35(Supp 2): e102, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34586216

RESUMEN

In the last five decades, considerable progress has been made towards understanding the etiology and pathogenesis of periodontal diseases and their interactions with the host. The impact of an individual periodontal condition on systemic homeostasis became more evident because of this knowledge and prompted advances in studies that associate periodontitis with systemic diseases and conditions. The term Periodontal Medicine describes how periodontal infection/inflammation can affect extraoral health. This review presents the current scientific evidence on the most investigated associations between periodontitis and systemic diseases and conditions, such as cardiovascular diseases, diabetes, preterm birth and low birth weight, and pneumonia. Additionally, other associations between periodontitis and chronic inflammatory bowel disease, colorectal cancer, and Alzheimer's disease that were recently published and are still poorly studied were described. Thus, the aim of this review was to answer the following question: What is the future of Periodontal Medicine? Epidemiological evidence and the evidence of biological plausibility between periodontitis and general health reinforce the rationale that the study of Periodontal Medicine should continue to advance, along with improvements in the epidemiological method, highlighting the statistical power of the studies, the method for data analysis, the case definition of periodontitis, and the type of periodontal therapy to be applied in intervention studies.


Asunto(s)
Diabetes Mellitus , Enfermedades Periodontales , Periodontitis , Nacimiento Prematuro , Femenino , Humanos , Recién Nacido , Enfermedades Periodontales/epidemiología , Enfermedades Periodontales/terapia , Periodoncia , Periodontitis/epidemiología , Periodontitis/terapia , Embarazo
14.
Georgian Med News ; (316-317): 70-73, 2021.
Artículo en Ruso | MEDLINE | ID: mdl-34511448

RESUMEN

Objective - to study the effect of braces on the dentoalveolar system and the occurrence of changes in the body as a whole. The study involved 25 patients, 15 women and 10 men aged 20 to 45 years, with an anomaly of occlusion of the dentition in the transversal and sagittal plane, crowded position of the teeth of the upper and lower dentition. All patients were treated orthodontically on the MBT prescription bracket system made of high-quality steel grade 17-4. In patients, the bracket system was fixed to the light (double) curing adhesive ENLIGHT by ORMCO. All patients signed an informed consent form. Orthodontic braces are used to treat dental anomalies. Increased microbial contamination (poor hygiene) due to the appearance of additional retention points for plaque, trauma to the oral mucosa with parts of orthodontic devices, invagination and infringement of the gingival papilla by moving teeth are the main factors in the development of gingivitis, periodontitis and cheilitis during treatment with a bracket system. As a result of the study, it was revealed that the proteins of non-specific immunity sIgA and lysozyme were studied in the mixed saliva of patients with a fixed metal bracket system. It was found that in 40% of patients after 3 months. after treatment, local immunodeficiency develops, which requires antibacterial therapy and professional oral hygiene in these terms. In conclusion, it is noted that braces have both positive and negative effects on the dental system and the body as a whole, so when planning orthodontic treatment, patients with initially reduced local immunity of the oral cavity should be included in the risk group.


Asunto(s)
Gingivitis , Soportes Ortodóncicos , Periodontitis , Femenino , Humanos , Masculino , Saliva
15.
Georgian Med News ; (316-317): 83-88, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34511451

RESUMEN

The method of dental implantation in patients with generalized periodontitis requires in-depth study of markers of bone remodeling, which will reveal the features of bone mineral density (BMD) and allow to predict the results of implantation in the above category of patients. The aim of the study was to evaluate the role of indicators of resorption and bone formation markers in predicting the results of dental implantation in patients with generalized periodontitis. We examined 240 patients with generalized periodontitis with partial adentia of the upper and lower jaws, who subsequently underwent comprehensive periodontal treatment using dental implantation methods. In the study of bone metabolism, it was found that 128 (53,4%) patients have normal BMD, and these patients are not at risk of osteoporosis in the cell bone, which caused the least number of complications (3,2%) of these patients. In 98 (40,8%) patients whose BMD corresponded to osteopenia, for 2 years of dispensary observations the number of periimplants and implant disintegration increased slightly, and the number of complications was 5,7%. In 14 (5,8%) patients whose BMD corresponded to osteoporosis, was observed the high activity of the resorptive process around the implants and their disintegration in 9,28% of cases.


Asunto(s)
Implantes Dentales , Periodontitis , Biomarcadores , Densidad Ósea , Remodelación Ósea , Implantación Dental , Humanos , Mandíbula , Periodontitis/diagnóstico
16.
Oral Health Prev Dent ; 19(1): 441-448, 2021 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-34505498

RESUMEN

PURPOSE: During the past 20 years, a plethora of research reports has been published showing a statistical association between poor oral health and cardiovascular diseases. The aim of this narrative review was to focus on associations between oral infections and non-atherosclerosis-related systemic diseases. MATERIALS AND METHODS: An open literature search and evaluation of articles were conducted on Medline and Cochrane databases with the key words 'oral infection', 'periodontitis', 'pneumonia', 'osteoarthritis', 'rheumatic diseases', 'inflammatory bowel disease', 'kidney disease', 'liver diseases', 'metabolic syndrome', 'diabetes', 'cancer', 'Alzheimer's disease'. Cardiovascular diseases were excluded from the analysis. RESULTS: The scarcity of controlled studies did not allow conducting a systematic review with meta-analysis on the topics, but dental infections have been shown be associated with several general diseases also beyond the atherosclerosis paradigm. However, there is no causal evidence of the role of dental infections in this regard. Poor oral health has nevertheless often been observed to be associated with worsening of the diseases and may also affect treatments. CONCLUSIONS: Maintaining good oral health is imperative regarding many diseases, and its importance in the daily life of any patient group cannot be over emphasised.


Asunto(s)
Enfermedades Cardiovasculares , Periodontitis , Enfermedades Cardiovasculares/epidemiología , Enfermedades Cardiovasculares/etiología , Humanos , Salud Bucal , Periodontitis/complicaciones , Periodontitis/epidemiología
17.
Int J Mol Sci ; 22(17)2021 Aug 25.
Artículo en Inglés | MEDLINE | ID: mdl-34502071

RESUMEN

We evaluated the role of allicin in periodontitis using an in silico and in vitro design. An in silico docking analysis was performed to assess the plausible interactions between allicin and PD-L1. The cytokine profile of gingival crevicular fluid (GCF) samples obtained from periodontitis patients was estimated by cytometric bead array. CD3+ lymphocytes isolated from the peripheral blood were sorted and characterized using immunomagnetic techniques. Cultured and expanded lymphocytes were treated with the GCF samples to induce T-cell exhaustion. Optimum concentrations of allicin were added to exhausted lymphocytes to compare the expression of TIM-3 and LAG-3 gene expression at baseline and post-treatment. Allicin was found to bind to the PD-L1 molecule as revealed by the in-silico experiment, which is possibly an inhibitory interaction although not proven. GCF from periodontitis patients had significantly higher concentrations of TNF-α, CCL2, IL-6, IFN-γ, and CXCL8 than controls. GCF treatment of CD3+ lymphocytes from the periodontitis patients significantly increased expression of T-cell exhaustion markers TIM-3 and LAG-3. Allicin administration with GCF treatment resulted in significant lowering of the expression of exhaustion markers. Allicin may exert an immunostimulatory role and reverse immune-destructive mechanisms such as T-cell exhaustion.


Asunto(s)
Antígeno B7-H1/metabolismo , Disulfuros/farmacología , Periodontitis/metabolismo , Ácidos Sulfínicos/farmacología , Linfocitos T/efectos de los fármacos , Antígenos CD/genética , Antígenos CD/metabolismo , Antígeno B7-H1/química , Sitios de Unión , Células Cultivadas , Quimiocina CCL2/genética , Quimiocina CCL2/metabolismo , Quimiocina CXCL6/genética , Quimiocina CXCL6/metabolismo , Disulfuros/química , Receptor 2 Celular del Virus de la Hepatitis A/genética , Receptor 2 Celular del Virus de la Hepatitis A/metabolismo , Humanos , Interferón gamma/genética , Interferón gamma/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , Unión Proteica , Ácidos Sulfínicos/química , Linfocitos T/metabolismo , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo
18.
Life Sci ; 284: 119938, 2021 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-34506837

RESUMEN

AIMS: The relationship between stress to endoplasmic reticulum (ER) and periodontitis has been known, and ER stress induced by Porphyromonas gingivalis results in the loss of alveolar bone. Salubrinal is a small synthetic compound and attenuates ER stress through inhibition of de-phosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2α). In this study, we examined whether salubrinal attenuates periodontitis in a mouse model of experimental periodontal disease. MATERIALS AND METHODS: We evaluated loss of alveolar bone and attachment levels in periodontium using micro-computed tomography (µCT) and hematoxylin-eosin (HE) staining, respectively. Furthermore, we measured osteoclast numbers using tartrate-resistant acid phosphatase (TRAP) staining and osteoblast numbers using HE staining for bone resorption and for bone formation, respectively. To examine the inhibitory effects of salubrinal against pro-inflammatory cytokines, we measured TNF-α and IL1-ß score in periodontium using immunohistostaining. KEY FINDINGS: The results revealed that salubrinal suppressed loss of alveolar bone and attachment levels in periodontium induced by periodontitis. It decreased osteoclast numbers and increased osteoblasts. It also suppressed the expression levels of TNF-α in periodontium. SIGNIFICANCE: These results show that salubrinal alleviates periodontitis through suppression of alveolar bone resorption and the pro-inflammatory cytokine, and promotion of the bone formation. Since salubrinal has been shown to have these beneficial effects for periodontal disease, it may provide a novel therapeutic possibility for the disease.


Asunto(s)
Pérdida de Hueso Alveolar/tratamiento farmacológico , Cinamatos/uso terapéutico , Tiourea/análogos & derivados , Pérdida de Hueso Alveolar/complicaciones , Pérdida de Hueso Alveolar/diagnóstico por imagen , Pérdida de Hueso Alveolar/patología , Animales , Recuento de Células , Cinamatos/administración & dosificación , Cinamatos/farmacología , Modelos Animales de Enfermedad , Estrés del Retículo Endoplásmico/efectos de los fármacos , Interleucina-1beta/metabolismo , Masculino , Ratones Endogámicos C57BL , Osteoblastos/efectos de los fármacos , Osteoblastos/patología , Periodontitis/complicaciones , Periodontitis/tratamiento farmacológico , Periodontitis/patología , Tiourea/administración & dosificación , Tiourea/farmacología , Tiourea/uso terapéutico , Factor de Transcripción CHOP/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Microtomografía por Rayos X
19.
Biomed Res Int ; 2021: 3278351, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34532500

RESUMEN

Recent studies have supported the relationship between periodontitis and carotid artery calcification (CAC), but still uncertain. This systematic review is aimed at evaluating the association between periodontitis and CAC. The search was conducted in four electronic databases: PubMed, EMBASE, Web of Science, and The Cochrane Library, supplemented by checking references of included articles and related review articles. Eligibility assessment and data extraction were conducted independently. The quality assessment and publication bias analysis were performed. The association between periodontitis and CAC was presented in odd ratio (OR) with 95% confidence interval (CI). Additional outcomes included the percentage of alveolar bone loss in CAC versus non-CAC. Twelve studies were included, and 10 were performed quantity analysis. Periodontitis with secure definition (OR = 2.02, 95%CI = 1.18 - 3.45) and insecure definition (OR = 10.78, 95%CI = 4.41 - 26.34) was associated with CAC. And a higher average percentage of alveolar bone loss (weighted mean difference = 10.84%; 95%CI = 6.40 - 15.48) was also observed in CAC patients compared to non-CAC patients. No significant publication bias was found. The results of this systematic review and meta-analysis revealed a significant relationship between periodontitis and CAC.


Asunto(s)
Calcinosis/fisiopatología , Arterias Carótidas/fisiopatología , Artropatías/fisiopatología , Periodontitis/fisiopatología , Enfermedades Vasculares/fisiopatología , Arterias Carótidas/metabolismo , Enfermedades de las Arterias Carótidas/fisiopatología , Enfermedad de la Arteria Coronaria/fisiopatología , Humanos , Oportunidad Relativa , Periodontitis/metabolismo , Medición de Riesgo/métodos , Factores de Riesgo
20.
BMC Oral Health ; 21(1): 426, 2021 09 04.
Artículo en Inglés | MEDLINE | ID: mdl-34481478

RESUMEN

BACKGROUND: B cell activating factor (BAFF) is a member of the tumor necrosis factor (TNF) superfamily with immunomodulatory effects on both innate and adaptive immune responses. Periodontitis is an inflammatory disease characterized by periodontal soft tissue inflammation and the progressive loss of periodontal ligament and alveolar bone. Macrophages are closely related to periodontitis progression. However, the role of BAFF in periodontitis development and macrophage polarization and the underlying mechanism remain unknown. METHODS: In vivo, a ligation-induced mouse model of periodontitis for BAFF blockade was established to investigate the expression of inducible nitric oxide synthase (iNOS) through real-time PCR (RT-PCR) and immunohistochemistry. In addition, the level of TNF-α in the periodontium, the number of osteoclasts, and alveolar bone resorption were observed. In vitro, RAW 264.7 macrophage cells were treated with 100 ng/mL Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) in either the presence or absence of 50 nM small interfering RNA (siRNA) targeting BAFF, followed by further incubation for 24 h. These cells and supernatants were collected and stored for RT-PCR, enzyme-linked immunosorbent assay, western blotting and immunofluorescence microscopy. RESULTS: In vivo, BAFF blockade decreased the levels of TNF-α in the periodontium in a ligature-induced mouse periodontitis model. Reduced osteoclast formation and lower alveolar bone loss were also observed. In addition, BAFF blockade was related to the expression of polarization signature molecules in macrophages. In vitro, BAFF knockdown notably suppressed the production of TNF-α in RAW 264.7 cells stimulated by P. gingivalis LPS. Moreover, BAFF knockdown attenuated the polarization of RAW 264.7 cells into classically activated macrophages (M1), with reduced expression of iNOS. CONCLUSIONS: Based on our limited evidence, we showed BAFF blockade exhibits potent anti-inflammatory properties in mice experimental periodontitis in vivo and in P. gingivalis LPS-treated RAW 264.7 cells in vitro, and macrophage polarization may be responsible for this effect.


Asunto(s)
Factor Activador de Células B , Periodontitis , Animales , Lipopolisacáridos , Macrófagos , Ratones , Porphyromonas gingivalis , Células RAW 264.7
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