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1.
Am J Med Sci ; 361(5): 655-658, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-34024355

RESUMEN

Adult-Onset Still's Disease (AOSD) usually presents with a salmon-colored skin rash and arthralgias. However, it can also be present with pleural and pericardial effusions. These effusions are often misdiagnosed as having an infectious etiology because AOSD usually present with fever, leukocytosis, elevated inflammatory markers, procalcitonin and CRP. There is usually a delay in giving steroids until the exclusion of all infectious etiologies, including extensive workups. Herein, we present a case report of AOSD in a patient with recurrent pleural and pericardial effusions, with no skin rashes or joint pain. Patient initially presented with fever, pleural and pericardial effusions, which was then treated as pneumonia with parapneumonic effusions. Patient returned for the second time with shortness of breath, productive cough, and fever, with no resolutions of pleural and pericardial effusions. Patient was found to have an extremely high ferritin levels, whereby a diagnosis of AOSD was made after excluding infection, malignancy and other rheumatological disorders based on the Yamaguchi criteria. AOSD is a rare disease with unusual presentation and diagnosis is often delayed. This case aimed to raise awareness among physicians of the multifaceted presentation of AOSD.


Asunto(s)
Derrame Pericárdico/diagnóstico , Derrame Pleural/diagnóstico , Enfermedad de Still del Adulto/diagnóstico , Anciano , Diagnóstico Diferencial , Femenino , Humanos , Derrame Pericárdico/etiología , Derrame Pericárdico/patología , Derrame Pleural/etiología , Derrame Pleural/patología , Recurrencia , Piel/patología , Enfermedad de Still del Adulto/complicaciones , Enfermedad de Still del Adulto/patología
2.
Pan Afr Med J ; 38: 236, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34046141

RESUMEN

Toxic epidermal necrolysis-like acute cutaneous lupus erythematosus (TEN-like ACLE) is a rare manifestation of systemic lupus erythematosus (SLE). Because of its rarity, little is known about this entity. In this report, we describe a case of two women previously diagnosed with SLE that presented TEN-like skin lesions. The common elements in both patients were the initial disposition of the lesions on the photoexposed areas, the positivity of Nikolsky´s sign, the discrete mucosal attrition compared to that observed during TEN, and the simultaneous appearance of dermatological lesions with an extra-cutaneous flare of lupus disease. The skin biopsy in both cases showed epidermal necrosis with an identification of lupus band on direct immunofluorescence. Systemic corticosteroids were used with a good evolution after 2 weeks. Skin damage is an indicator of disease activity, and careful search for extracutaneous involvement is obligatory to prevent further complications.


Asunto(s)
Corticoesteroides/administración & dosificación , Lupus Eritematoso Cutáneo/diagnóstico , Síndrome de Stevens-Johnson/diagnóstico , Enfermedad Aguda , Adulto , Biopsia , Femenino , Humanos , Lupus Eritematoso Cutáneo/tratamiento farmacológico , Lupus Eritematoso Cutáneo/patología , Necrosis/patología , Piel/patología , Síndrome de Stevens-Johnson/patología
5.
Front Immunol ; 12: 672808, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34012456

RESUMEN

The anti-inflammatory role of extra-adrenal glucocorticoid (GC) synthesis at epithelial barriers is of increasing interest with regard to the search for alternatives to synthetic corticosteroids in the therapy of inflammatory disorders. Despite being very effective in many situations the use of synthetic corticosteroids is often controversial, as exemplified in the treatment of influenza patients and only recently in the current COVID-19 pandemic. Exploring the regulatory capacity of locally produced GCs in balancing immune responses in barrier tissues and in pathogenic disorders that lead to symptoms in multiple organs, could provide new perspectives for drug development. Intestine, skin and lung represent the first contact zones between potentially harmful pathogens or substances and the body, and are therefore important sites of immunoregulatory mechanisms. Here, we review the role of locally produced GCs in the regulation of type 2 immune responses, like asthma, atopic dermatitis and ulcerative colitis, as well as type 1 and type 3 infectious, inflammatory and autoimmune diseases, like influenza infection, psoriasis and Crohn's disease. In particular, we focus on the role of locally produced GCs in the interorgan communication, referred to as gut-skin axis, gut-lung axis or lung-skin axis, all of which are interconnected in the pathogenic crosstalk atopic march.


Asunto(s)
Glucocorticoides/inmunología , Mucosa Intestinal/inmunología , Pulmón/inmunología , Piel/inmunología , Antiinflamatorios , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/patología , Dermatitis Atópica/inmunología , Dermatitis Atópica/patología , Epitelio/inmunología , Glucocorticoides/biosíntesis , Humanos , Inflamación , Mucosa Intestinal/patología , Pulmón/patología , Piel/patología
6.
Zhonghua Shao Shang Za Zhi ; 37(5): 495-500, 2021 May 20.
Artículo en Chino | MEDLINE | ID: mdl-34044531

RESUMEN

Skin is an important defense barrier of human body and one of the most vulnerable organs. Wounds are the result of damage to the integrity of skin. Chronic wounds and hypertrophic scar formation are the results of abnormal wound healing, and are also the clinical problems those need to be resolved urgently in the field of wound repair. In recent years, researchers have found that mesenchymal stem cells (MSCs) can promote wound healing, improve wound healing quality, and reduce scar formation. The therapeutic effect of MSCs may be derived from the exosomes derived from them. This paper reviews the research advances of exosomes derived from MSCs in wound healing and prevention and treatment of hypertrophic scars in recent years and looks up to the prospect for the clinical application.


Asunto(s)
Cicatriz Hipertrófica , Exosomas , Células Madre Mesenquimatosas , Cicatriz Hipertrófica/patología , Cicatriz Hipertrófica/prevención & control , Humanos , Piel/patología , Cicatrización de Heridas
7.
Medicine (Baltimore) ; 100(18): e25770, 2021 May 07.
Artículo en Inglés | MEDLINE | ID: mdl-33950967

RESUMEN

INTRODUCTION: CD30+ primary cutaneous anaplastic large cell lymphoma (PC-ALCL) is a rare T-cell neoplasm, and has been reported to present with an indolent behavior. The PC-ALCL with aggressive behavior has not been reported in the literature. PATIENT CONCERNS: We treated a patient with PC-ALCL that exhibited indolent behavior in the past 2 years and aggressive behavior within the last 3 months before presentation. DIAGNOSIS: Aggressive CD30+ primary cutaneous anaplastic large cell lymphoma. INTERVENTIONS: The radiotherapy regimen was individualized in terms of the target volume delineation and dose prescription, and the dose-response relationship was evaluated. OUTCOMES: The mean distance of microscopic infiltration was 14.1 mm in depth and 14.3 mm circumferentially. The lesion completely regressed after the delivery of 40 Gy in 20 fractions over 4 weeks. The tumor did not recur over the next year. CONCLUSION: An aggressive disease course is rare for indolent CD30+ PC-ALCL, which has similar histopathological characteristics as indolent PC-ALCL. The radiotherapy strategy should be individualized with curative intent.


Asunto(s)
Fraccionamiento de la Dosis de Radiación , Linfoma Anaplásico Cutáneo Primario de Células Grandes/radioterapia , Neoplasias Cutáneas/radioterapia , Piel/patología , Anciano de 80 o más Años , Humanos , Linfoma Anaplásico Cutáneo Primario de Células Grandes/diagnóstico , Linfoma Anaplásico Cutáneo Primario de Células Grandes/patología , Imagen por Resonancia Magnética , Masculino , Estadificación de Neoplasias , Dosificación Radioterapéutica , Piel/diagnóstico por imagen , Neoplasias Cutáneas/diagnóstico , Neoplasias Cutáneas/patología , Tomografía Computarizada por Rayos X , Resultado del Tratamiento
8.
Anticancer Res ; 41(5): 2485-2488, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33952475

RESUMEN

BACKGROUND/AIM: Cutaneous manifestations of disease are exceedingly rare and commonly overlooked in clinical practice. Allergies or contact dermatitis, autoimmune disease or skin cancer are the most common conditions typically associated with skin lesions. Rarely, cutaneous lesions may be the first sign of internal malignancy, or even resemble recurrent disease in those with history of cancer. CASE REPORT: Herein, we report a case of an otherwise healthy male who presented to his primary care provider (PCP) with a skin lesion misdiagnosed as a furuncle, which eventually led to diagnosis of metastatic esophageal cancer. The patient was a 64-year-old male, presenting with a fungating lesion on the tip of his nose which was biopsied, confirming adenocarcinoma likely from a gastrointestinal source. Staging imaging showed extensive lung, liver, and boney metastatic disease. He was initially treated with chemotherapy and trastuzumab. CONCLUSION: Cutaneous lesions are a rare presenting sign of malignancy, but rapidly growing lesions should be evaluated for possible metastatic disease.


Asunto(s)
Adenocarcinoma/diagnóstico , Neoplasias Esofágicas/diagnóstico , Nariz/diagnóstico por imagen , Piel/diagnóstico por imagen , Adenocarcinoma/diagnóstico por imagen , Adenocarcinoma/patología , Neoplasias Esofágicas/diagnóstico por imagen , Neoplasias Esofágicas/patología , Humanos , Masculino , Persona de Mediana Edad , Nariz/patología , Piel/patología
9.
Biomed Res Int ; 2021: 6695663, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33937411

RESUMEN

Background: When vascular endothelial cells are subjected to external stimuli, paracrine hormones and cytokines act on adjacent cells. The regulation of the biological behaviour of cells is closely related to the maintenance of organ function and the occurrence and development of disease. However, it is unclear whether vascular endothelial cells affect the biological behaviour of cells involved in wound repair through autocrine and paracrine mechanisms and ultimately play a role in wound healing. We aimed to verify the effect of the autocrine and paracrine functions of vascular endothelial cells on wound healing. Materials and Methods: ELISA was used to detect platelet-derived growth factor, basic fibroblast growth factor, epidermal growth factor, and vascular endothelial growth factor in human umbilical vascular endothelial cell-conditioned medium (HUVEC-CM). Different concentrations of HUVEC-CM were used to treat different stem cells. CCK-8 and scratch assays were used to detect the proliferation and migration ability of each cell. A full-thickness dorsal skin defect model was established in mice, and skin wound healing was observed after the local injection of HUVEC-CM, endothelial cell medium (ECM), or normal saline. H&E staining and immunofluorescence were used to observe the gross morphology of the wound tissue, the epithelial cell migration distance, and the expression of CD3 and CD31. Results: HUVEC-CM promotes the proliferation and migration of epidermal stem cells, skin fibroblasts, bone marrow mesenchymal stem cells, and HUVECs themselves. Furthermore, HUVEC-CM can promote angiogenesis in mouse skin wounds and granulation tissue formation and can accelerate wound surface epithelialization and collagen synthesis, thereby promoting wound healing. Conclusion: Our results clearly suggest that it is practicable and effective to promote wound healing with cytokines secreted by vascular endothelial cells in a mouse model.


Asunto(s)
Comunicación Autocrina , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Comunicación Paracrina , Piel/patología , Cicatrización de Heridas , Antígenos CD/metabolismo , Comunicación Autocrina/efectos de los fármacos , Biomarcadores/metabolismo , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Medios de Cultivo Condicionados/farmacología , Citocinas/metabolismo , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Células Madre Embrionarias Humanas/citología , Células Madre Embrionarias Humanas/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Humanos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/efectos de los fármacos , Comunicación Paracrina/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos
10.
Int J Mol Sci ; 22(9)2021 Apr 29.
Artículo en Inglés | MEDLINE | ID: mdl-33947121

RESUMEN

Dermal wound healing describes the progressive repair and recalcitrant mechanism of 12 damaged skin, and eventually, reformatting and reshaping the skin. Many probiotics, nutritional supplements, metal nanoparticles, composites, skin constructs, polymers, and so forth have been associated with the improved healing process of wounds. The exact mechanism of material-cellular interaction is a point of immense importance, particularly in pathological conditions such as diabetes. Bioengineered alternative agents will likely continue to dominate the outpatient and perioperative management of chronic, recalcitrant wounds as new products continue to cut costs and improve the wound healing process. This review article provides an update on the various remedies with confirmed wound healing activities of metal-based nanoceutical adjuvanted agents and also other nano-based counterparts from previous experiments conducted by various researchers.


Asunto(s)
Adyuvantes Farmacéuticos/uso terapéutico , Nanomedicina/tendencias , Nanopartículas/uso terapéutico , Cicatrización de Heridas/efectos de los fármacos , Antiinfecciosos Locales/uso terapéutico , Vendajes , Materiales Biocompatibles , Humanos , Hidrogeles , Neovascularización Fisiológica , Fitoterapia , Repitelización , Regeneración , Piel/inmunología , Piel/lesiones , Piel/patología , Fenómenos Fisiológicos de la Piel , Trasplante de Piel , Técnicas de Cierre de Heridas , Infección de Heridas/prevención & control
11.
Clin Dermatol ; 39(1): 149-162, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33972045

RESUMEN

Skin is one of target organs affected by the novel coronavirus SARS-CoV-2, and in response to the current COVID-19 pandemic, a fast body of literature has emerged on related cutaneous manifestations. Current perspective is that the skin is not only a bystander of the general cytokines storm with thrombophilic multiorgan injury, but it is directly affected by the epithelial tropism of the virus, as confirmed by the detection of SARS-CoV-2 in endothelial cells and epithelial cells of epidermis and eccrine glands. In contrast with the abundance of epidemiologic and clinical reports, histopathologic characterization of skin manifestations is limited. Without an adequate clinicopathologic correlation, nosology of clinically similar conditions is confusing, and effective association with COVID-19 remains presumptive. Several patients with different types of skin lesions, including the most specific acral chilblains-like lesions, showed negative results at SARS-CoV-2 nasopharyngeal and serologic sampling. The aim of this review is to provide an overview of what has currently been reported worldwide, with a particular emphasis on microscopic patterns of the skin manifestations in patients exposed to or affected by COVID-19. Substantial breakthroughs may occur in the near future from more skin biopsies, improvement of immunohistochemistry studies, RNA detection of SARS-CoV-2 strain by real-time polymerase chain reaction-based assay, and electron microscopic studies.


Asunto(s)
/complicaciones , Enfermedades de la Piel/patología , Enfermedades de la Piel/virología , Piel/patología , Eritema Pernio/patología , Eritema Pernio/virología , Eritema Multiforme/patología , Eritema Multiforme/virología , Exantema/patología , Exantema/virología , Humanos , Necrosis/virología , Púrpura/patología , Púrpura/virología , Síndrome de Respuesta Inflamatoria Sistémica/complicaciones , Urticaria/patología , Urticaria/virología
12.
Int J Mol Sci ; 22(9)2021 Apr 30.
Artículo en Inglés | MEDLINE | ID: mdl-33946233

RESUMEN

Neoplasms derived from follicular tissue are extremely rare. Clinically, they are reported as non-symptomatic, slow-growing nodules. These lesions are mainly benign, but the malignant type can occur. Mainly middle-aged people (50-60 years of age) are affected. These carcinomas are mainly localized on the head and neck or torso. They can be locally aggressive and infiltrate surrounding tissue and metastasize to regional lymph nodes. In the minority of cases, distant metastases are diagnosed. Quick and relevant diagnosis is the basis of a treatment for all types of tumors. The patient's life expectancy depends on multiple prognostic factors, including the primary tumor size and its mitotic count. Patients should be referred to a specialized skin cancer center to receive optimal multidisciplinary treatment. This article tries to summarize all the information that is currently available about pathogenesis, diagnosis, and treatment methods of follicular tumors.


Asunto(s)
Neoplasias Glandulares y Epiteliales/diagnóstico , Neoplasias Cutáneas/diagnóstico , Animales , Carcinogénesis/patología , Manejo de la Enfermedad , Humanos , Neoplasias Glandulares y Epiteliales/patología , Neoplasias Glandulares y Epiteliales/terapia , Piel/patología , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/terapia
14.
Medicine (Baltimore) ; 100(20): e25955, 2021 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-34011077

RESUMEN

RATIONALE: Mucous membrane pemphigoid (MMP) is a rare, autoimmune bullous disease that affects mucosal surfaces and skin. Early and aggressive treatment initiation may be warranted due to the risks of serious complications. However, it can be challenging to make an initial diagnosis. Viral infection such as hepatitis B virus (HBV) infection has been found to be associated with the formation of autoimmune bullous diseases. PATIENT CONCERNS: The patient was a 43-year-old male with gingivitis and recurrent swelling over the neck, cheeks, lips, and eyelids. The patient presented at oral medicine, otolaryngology, plastic surgery, and ophthalmology sequentially, and was later referred to the rheumatology, dermatology, and family medicine departments. Recurrent hemorrhagic bullae on oral mucosa and skin scarring occurred 2 years after the onset of the initial symptoms. DIAGNOSIS: Skin biopsy with direct immunofluorescence was performed under the suspicion of MMP. Lesional hematoxylin and eosin stain and perilesional direct immunofluorescence were consistent with MMP. INTERVENTIONS: Systemic Prednisolone and topical corticosteroid were used to control the disease. OUTCOMES: A flare-up of hepatitis B developed as a result of systemic prednisolone use. The disease went through relapses and remissions. The patient is on low-dose prednisolone (5 mg/day) with a monthly outpatient visit in the family medicine department. LESSONS: It would be useful for medical practitioners in different specialties to be alert of the heterogeneous presentations of MMP. Chronic HBV infection might be a risk factor for MMP. In patients with chronic HBV infection, treatment of MMP must be closely monitored for the risk of reactivation of HBV.


Asunto(s)
Hepatitis B Crónica/complicaciones , Penfigoide Benigno de la Membrana Mucosa/diagnóstico , Prednisolona/administración & dosificación , Adulto , Biopsia , Relación Dosis-Respuesta a Droga , Hepatitis B Crónica/diagnóstico , Hepatitis B Crónica/inmunología , Humanos , Masculino , Mucosa Bucal/inmunología , Mucosa Bucal/patología , Penfigoide Benigno de la Membrana Mucosa/tratamiento farmacológico , Penfigoide Benigno de la Membrana Mucosa/inmunología , Penfigoide Benigno de la Membrana Mucosa/patología , Prednisolona/efectos adversos , Piel/inmunología , Piel/patología , Brote de los Síntomas
15.
Medicine (Baltimore) ; 100(16): e25433, 2021 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-33879674

RESUMEN

ABSTRACT: Discoid lupus erythematosus (DLE) is the most common skin manifestation of lupus; however, the molecular mechanisms underlying DLE remain unknown. Therefore, we aimed to identify key differentially expressed genes (DEGs) in discoid lupus skin and investigate their potential pathways.To identify candidate genes involved in the occurrence and development of the disease, we downloaded the microarray datasets GSE52471 and GSE72535 from the Gene Expression Database (GEO). DEGs between discoid lupus skin and normal controls were selected using the GEO2R tool and Venn diagram software (http://bioinformatics.psb.ugent.be/webtools/Venn/). The Database for Annotation, Visualization, and Integrated Discovery (DAVID), Enrichr, and Cytoscape ClueGo were used to analyze the Kyoto Encyclopedia of Gene and Genome pathways and gene ontology. Protein-protein interactions (PPIs) of these DEGs were further assessed using the Search Tool for the Retrieval Interacting Genes version 10.0.Seventy three DEGs were co-expressed in both datasets. DEGs were predominantly upregulated in receptor signaling pathways of the immune response. In the PPI network, 69 upregulated genes were selected. Furthermore, 4 genes (CXCL10, ISG15, IFIH1, and IRF7) were found to be significantly upregulated in the RIG-I-like receptor signaling pathway, from analysis of Enrichr and Cytoscape ClueGo.The results of this study may provide new insights into the potential molecular mechanisms of DLE. However, further experimentation is required to confirm these findings.


Asunto(s)
Redes Reguladoras de Genes/inmunología , Lupus Eritematoso Discoide/genética , Quimiocina CXCL10/genética , Biología Computacional , Citocinas/genética , Proteína 58 DEAD Box/metabolismo , Conjuntos de Datos como Asunto , Perfilación de la Expresión Génica , Humanos , Factor 7 Regulador del Interferón/genética , Helicasa Inducida por Interferón IFIH1/genética , Lupus Eritematoso Discoide/epidemiología , Lupus Eritematoso Discoide/inmunología , Lupus Eritematoso Discoide/patología , Análisis de Secuencia por Matrices de Oligonucleótidos , Mapeo de Interacción de Proteínas , Mapas de Interacción de Proteínas/genética , Mapas de Interacción de Proteínas/inmunología , Receptores Inmunológicos/metabolismo , Transducción de Señal/genética , Transducción de Señal/inmunología , Piel/inmunología , Piel/patología , Programas Informáticos , Ubiquitinas/genética , Regulación hacia Arriba/inmunología
16.
Mar Drugs ; 19(5)2021 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-33922418

RESUMEN

Scleroderma is an autoimmune disease caused by the abnormal regulation of extracellular matrix synthesis and is activated by non-regulated inflammatory cells and cytokines. Echinochrome A (EchA), a natural pigment isolated from sea urchins, has been demonstrated to have antioxidant activities and beneficial effects in various disease models. The present study demonstrates for the first time that EchA treatment alleviates bleomycin-induced scleroderma by normalizing dermal thickness and suppressing collagen deposition in vivo. EchA treatment reduces the number of activated myofibroblasts expressing α-SMA, vimentin, and phosphorylated Smad3 in bleomycin-induced scleroderma. In addition, it decreased the number of macrophages, including M1 and M2 types in the affected skin, suggesting the induction of an anti-inflammatory effect. Furthermore, EchA treatment markedly attenuated serum levels of inflammatory cytokines, such as tumor necrosis factor-α and interferon-γ, in a murine scleroderma model. Taken together, these results suggest that EchA is highly useful for the treatment of scleroderma, exerting anti-fibrosis and anti-inflammatory effects.


Asunto(s)
Antiinflamatorios/farmacología , Macrófagos/efectos de los fármacos , Miofibroblastos/efectos de los fármacos , Naftoquinonas/farmacología , Esclerodermia Sistémica/prevención & control , Piel/efectos de los fármacos , Actinas/metabolismo , Animales , Bleomicina , Colágeno/metabolismo , Citocinas/metabolismo , Modelos Animales de Enfermedad , Fibrosis , Humanos , Mediadores de Inflamación/metabolismo , Macrófagos/inmunología , Macrófagos/metabolismo , Macrófagos/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Miofibroblastos/inmunología , Miofibroblastos/metabolismo , Miofibroblastos/patología , Fosforilación , Células RAW 264.7 , Esclerodermia Sistémica/inducido químicamente , Esclerodermia Sistémica/inmunología , Esclerodermia Sistémica/metabolismo , Piel/inmunología , Piel/metabolismo , Piel/patología , Proteína smad3/metabolismo , Vimentina/metabolismo
17.
Int J Mol Sci ; 22(7)2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33805585

RESUMEN

Extracellular vesicles (EVs) have an important role in mediating intercellular signaling in inflammation and affect the kinetics of wound healing, however, an understanding of the mechanisms regulating these responses remains limited. Therefore, we have focused on the use of cutaneous injury models in which to study the biology of EVs on the inflammatory phase of wound healing. For this, the foreign body response using sterile subcutaneous polyvinylalcohol (PVA) sponges is ideally suited for the parallel analysis of immune cells and EVs without the need for tissue dissociation, which would introduce additional variables. We have previously used this model to identify mediators of EV biogenesis, establishing that control of how EVs are made affects their payload and biological activity. These studies in normal mice led us to consider how conditions such as immunodeficiency and obsesity affect the profile of immune cells and EVs in this model using genetically defined mutant mice. Since EVs are intrinsically heterogenous in biological fluids, we have focused our studies on a novel technology, vesicle flow cytometry (vFC) to quantify changes in EVs in mouse models. Here, we show that myeloid-derived immune cells and EVs express proteins relevant in antigen presentation in PVA sponge implants that have distinct profiles in wildtype, immune-deficient (NOD scid) vs. diabetic (Leprdb) mice. Together, these results establish a foundation for the parallel analysis of both immune cells and EVs with technologies that begin to address the heterogeneity of intercellular communication in the wound bed.


Asunto(s)
Antígenos CD/inmunología , Vesículas Extracelulares/fisiología , Piel/lesiones , Piel/patología , Animales , Antígenos CD/genética , Antígenos CD/metabolismo , Diabetes Mellitus Experimental/inmunología , Modelos Animales de Enfermedad , Vesículas Extracelulares/inmunología , Vesículas Extracelulares/patología , Cinética , Masculino , Ratones Endogámicos C57BL , Ratones Endogámicos NOD/genética , Ratones Endogámicos NOD/inmunología , Ratones Obesos/inmunología , Células Mieloides/inmunología , Alcohol Polivinílico , Cicatrización de Heridas/inmunología , Cicatrización de Heridas/fisiología
18.
Zhonghua Shao Shang Za Zhi ; 37(4): 301-305, 2021 Apr 20.
Artículo en Chino | MEDLINE | ID: mdl-33874707

RESUMEN

In clinical work, it is observed that keloid has significantly different characteristics from other types of scar such as hypertrophic scar. The growth of keloid usually exceeds the margin of original wound and continuously invades the surrounding skin, and keloid has a certain recurrence rate after various treatment measures such as surgery and glucocorticoid injection, etc. The above phenomenon suggests that keloid has certain tumor characteristics, and we cannot judge keloid from the perspective of scar alone. This article attempts to re-describe the pathogenesis of keloid from the perspective of tumor and summarizes the tumor characteristics of keloid from self-sufficiency of growth signal, avoidance of apoptosis, and abnormal angiogenesis, etc.


Asunto(s)
Cicatriz Hipertrófica , Queloide , Neoplasias , Apoptosis , Cicatriz Hipertrófica/patología , Humanos , Queloide/patología , Piel/patología
19.
Int J Mol Sci ; 22(5)2021 Mar 07.
Artículo en Inglés | MEDLINE | ID: mdl-33799959

RESUMEN

Mastocytosis is characterized by the pathological accumulation of mast cells (MC) in various organs. In these patients, MC may degranulate and thereby contribute to clinical symptoms, especially when a concomitant allergy is present. However, MC activation can not only be induced by high-affinity receptors for IgE, but also by anaphylatoxins, neuropeptides, IgG immune complexes, complement-components, drugs, products of bacteria or parasites, as well as physical factors such as heat, cold, vibration, stress, sun, or physical effort. Symptoms due to mediators released by activated MC may develop in adults suffering from systemic mastocytosis, but also evolve in children who usually have cutaneous mastocytosis (CM). Clinically, CM is otherwise characterized by typical brown, maculopapular skin lesions or mastocytoma associated with a positive Darier's sign. Pruritus and flushing are common and blistering may also be recorded, especially in diffuse CM (DCM). Pediatric patients with mastocytosis may also have gastrointestinal, respiratory, and neurologic complaints. Although anaphylaxis is not a typical finding, pediatric patients with massive skin involvement and high tryptase levels have a relatively high risk to develop anaphylaxis. This paper reviews MC mediator-related symptoms and anaphylaxis in children with mastocytosis, with special emphasis on risk factors, triggers, and management.


Asunto(s)
Anafilaxia/etiología , Mastocitos/metabolismo , Mastocitosis/diagnóstico , Mastocitosis/etiología , Administración Tópica , Adulto , Niño , Humanos , Mordeduras y Picaduras de Insectos/etiología , Mastocitos/inmunología , Mastocitosis/complicaciones , Mastocitosis/terapia , Mastocitosis Sistémica/etiología , Factores de Riesgo , Piel/patología , Triptasas/genética
20.
Mutat Res ; 865: 503314, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33865536

RESUMEN

The reconstructed skin micronucleus (RSMN) assay was developed in 2006, as an in vitro alternative for genotoxicity evaluation of dermally applied chemicals or products. In the years since, significant progress has been made in the optimization of the assay, including publication of a standard protocol and extensive validation. However, the diverse morphology of skin cells makes cell preparation and scoring of micronuclei (MN) tedious and subjective, thus requiring a high level of technical expertise for evaluation. This ultimately has a negative impact on throughput and the assay would benefit by the development of an automated method which could reduce scoring subjectivity while also improving the robustness of the assay by increasing the number of cells that can be scored. Imaging flow cytometry (IFC) with the ImageStream®X Mk II can capture high-resolution transmission and fluorescent imagery of cells in suspension. This proof-of-principle study describes protocol modifications that enable such automated measurement in 3D skin cells following exposure to mitomycin C and colchicine. IFC was then used for automated image capture and the Amnis® Artificial Intelligence (AAI) software permitted identification of binucleated (BN) cells with 91% precision. On average, three times as many BN cells from control samples were evaluated using IFC compared to the standard manual analysis. When IFC MNBN cells were visually scored from within the BN cell images, their frequency compared well with manual slide scoring, showing that IFC technology can be applied to the RSMN assay. This method enables faster time to result than microscope-based scoring and the initial studies presented here demonstrate its capability for the detection of statistically significant increases in MNBN frequencies. This work therefore demonstrates the feasibility of combining IFC and AAI to automate scoring for the RSMN assay and to improve its throughput and statistical robustness.


Asunto(s)
Aprendizaje Profundo , Citometría de Flujo/métodos , Procesamiento de Imagen Asistido por Computador/métodos , Piel/patología , Inteligencia Artificial , Automatización de Laboratorios/instrumentación , Automatización de Laboratorios/métodos , Reacciones Falso Positivas , Estudios de Factibilidad , Citometría de Flujo/instrumentación , Humanos , Procesamiento de Imagen Asistido por Computador/instrumentación , Pruebas de Micronúcleos/instrumentación , Pruebas de Micronúcleos/métodos , Mitomicina/toxicidad , Modelos Biológicos , Pruebas de Mutagenicidad/instrumentación , Pruebas de Mutagenicidad/métodos , Valor Predictivo de las Pruebas , Prueba de Estudio Conceptual , Piel/diagnóstico por imagen , Piel Artificial , Programas Informáticos , Andamios del Tejido
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