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1.
Molecules ; 26(7)2021 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-33808054

RESUMEN

The main protease (Mpro) is a major protease having an important role in viral replication of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel coronavirus that caused the pandemic of 2020. Here, active Mpro was obtained as a 34.5 kDa protein by overexpression in E. coli BL21 (DE3). The optimal pH and temperature of Mpro were 7.5 and 37 °C, respectively. Mpro displayed a Km value of 16 µM with Dabcyl-KTSAVLQ↓SGFRKME-Edans. Black garlic extract and 49 polyphenols were studied for their inhibitory effects on purified Mpro. The IC50 values were 137 µg/mL for black garlic extract and 9-197 µM for 15 polyphenols. The mixtures of tannic acid with puerarin, daidzein, and/or myricetin enhanced the inhibitory effects on Mpro. The structure-activity relationship of these polyphenols revealed that the hydroxyl group in C3', C4', C5' in the B-ring, C3 in the C-ring, C7 in A-ring, the double bond between C2 and C3 in the C-ring, and glycosylation at C8 in the A-ring contributed to inhibitory effects of flavonoids on Mpro.


Asunto(s)
/antagonistas & inhibidores , Polifenoles/química , Polifenoles/farmacología , Inhibidores de Proteasas/farmacología , /genética , Dimetilsulfóxido/farmacología , Sinergismo Farmacológico , Ajo/química , Concentración de Iones de Hidrógeno , Extractos Vegetales/farmacología , Plantas/química , Inhibidores de Proteasas/química , Relación Estructura-Actividad , Temperatura
2.
Food Chem ; 351: 129324, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33647694

RESUMEN

Conjugation between peptides and polyphenols could improve their bioactive and functional properties. The improvement effects of anchovy protein hydrolysates (APH) -polyphenol (catechin (CA), gallic acid (GA), tannic acid (TA)) conjugates were investigated. The content of protein and polyphenols and ratio of polyphenols/peptides in conjugates increased as the number of OH group increased with TA > CA > GA. Results showed that APH-CA and APH-GA exhibited the highest ORAC and ABTS+ scavenging capacity, respectively. Mass spectrometry analysis suggested the highest number of bioactive peptides were identified in APH-CA 5:1 (APH/polyphenols). The physical stability of fish oil emulsions during storage was significantly enhanced by TA 5:1 conjugate followed by CA 5:1 conjugate. The oxidative stability was remarkably elevated by APH-GA 10:1. This was due to the antioxidant capacity and the peptides adsorbed at the interfacial. This study demonstrated that APH-polyphenol conjugates could bring the possibility of utilizing peptides-polyphenols in the nutraceutical and functional food ingredient fields.


Asunto(s)
Antioxidantes/química , Aceites de Pescado/química , Proteínas de Peces/química , Polifenoles/química , Animales , Catequina/química , Emulsiones , Hidrólisis , Oxidación-Reducción , Taninos/química
3.
Food Chem ; 351: 129273, 2021 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-33662907

RESUMEN

Heat processing of ready-to-drink beverages is required to ensure a microbiologically safe product, however, this can result in the loss of bioactive compounds responsible for functionality. The objective of this study was to establish the thermal stability of a novel dihydrochalcone, 3',5'-di-ß-d-glucopyranosyl-3-hydroxyphloretin (2), 3',5'-di-ß-d-glucopyranosylphloretin (3) and other Cyclopia subternata phenolic compounds, in model solutions with or without citric acid and ascorbic acid. The solutions were heated at 93, 121 and 135 °C, relevant to pasteurisation, commercial sterilisation and ultra-high temperature (UHT) pasteurisation, respectively. For most compounds, the acids decreased the second order reaction rate constants, up to 27 times. Compound 2 (46.29 ± 0.53 (g/100 g)-1 h-1), and to a lesser extent compound 3 (5.94 ± 0.01 (g/100 g)-1 h-1) were the most thermo-unstable compounds when treated at 135 °C without added acids. Even though differential effects were observed for compounds at different temperatures and formulations, overall, the phenolic compounds were most stable under UHT pasteurisation conditions.


Asunto(s)
Bebidas/análisis , Chalconas/química , Fabaceae/química , Extractos Vegetales/química , Polifenoles/química , Temperatura , Glicosilación , Pasteurización , Fenoles/análisis , Soluciones
4.
Molecules ; 26(5)2021 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-33652639

RESUMEN

Hepatitis C is affecting millions of people around the globe annually, which leads to death in very high numbers. After many years of research, hepatitis C virus (HCV) remains a serious threat to the human population and needs proper management. The in silico approach in the drug discovery process is an efficient method in identifying inhibitors for various diseases. In our study, the interaction between Epigallocatechin-3-gallate, a component of green tea, and envelope glycoprotein E2 of HCV is evaluated. Epigallocatechin-3-gallate is the most promising polyphenol approved through cell culture analysis that can inhibit the entry of HCV. Therefore, various in silico techniques have been employed to find out other potential inhibitors that can behave as EGCG. Thus, the homology modelling of E2 protein was performed. The potential lead molecules were predicted using ligand-based as well as structure-based virtual screening methods. The compounds obtained were then screened through PyRx. The drugs obtained were ranked based on their binding affinities. Furthermore, the docking of the topmost drugs was performed by AutoDock Vina, while its 2D interactions were plotted in LigPlot+. The lead compound mms02387687 (2-[[5-[(4-ethylphenoxy) methyl]-4-prop-2-enyl-1,2,4-triazol-3-yl] sulfanyl]-N-[3(trifluoromethyl) phenyl] acetamide) was ranked on top, and we believe it can serve as a drug against HCV in the future, owing to experimental validation.


Asunto(s)
Catequina/análogos & derivados , Hepacivirus/efectos de los fármacos , Hepatitis C/tratamiento farmacológico , Proteínas del Envoltorio Viral/genética , Antivirales/química , Antivirales/farmacología , Catequina/química , Catequina/farmacología , Hepacivirus/genética , Hepacivirus/patogenicidad , Hepatitis C/virología , Humanos , Ligandos , Simulación del Acoplamiento Molecular , Polifenoles/química , Polifenoles/farmacología , Té/química , Proteínas del Envoltorio Viral/antagonistas & inhibidores , Internalización del Virus/efectos de los fármacos
5.
Food Chem ; 352: 129376, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-33662917

RESUMEN

In the present study, three types of polyphenols, namely, (-)-epigallocatechin-3-gallate (EGCG), catechin (C), and gallic acid (GA), were grafted to myofibrillar protein (MP)-dextran (DX) conjugate through a free radical-mediated adduction method. The analysis of secondary structure showed that conjugation of polyphenols induced a decrease in contents of α-helix structures. The surface hydrophobicity of MP-DX conjugate was increased after polyphenols were covalently adducted, while that of the free amino, thiol groups, and tyrosine residues were decreased, especially with the addition of EGCG (p < 0.05). Analysis of rheological properties showed that covalently linking of polyphenols decreased the thermal gelling capacity by inhibiting myosin-head aggregation and myosin tails interaction. Moreover, polyphenol adduction was able to remarkably improve the thermal stability and antioxidant activity of MP-DX conjugate. The findings regarding enhanced functionalities evidence potential of applying the ternary adduct as a novel antioxidant.


Asunto(s)
Antioxidantes/química , Dextranos/química , Calor , Proteínas Musculares/química , Polifenoles/química , Estabilidad Proteica , Estructura Secundaria de Proteína
6.
Food Chem ; 349: 129118, 2021 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-33556725

RESUMEN

Brazilin (Bra), hematoxylin (Hto) and hematein (Hte) are structurally similar polyphenols having rich biological activities, but their antioxidant ability has not been well studied. Here, their protective ability against human serum albumin (HSA) oxidative degradation were investigated using 2,2'-Azobis (2-methylpropionamidine) dihydrochloride (AAPH), NaClO and Fenton like reactions methods. The results indicated that polyphenols inhibited the oxidative injuries of HSA in the order: Hto > Bra > Hte. Additionally, the biological effects of polyphenols were mostly influenced by their binding to protein. Therefore, the structure-affinity relationships of polyphenols binding to HSA were also explored. Fluorescence experiments indicated that polyphenols bound to HSA through static quenching mechanism. Furthermore, some conformational changes of HSA could be observed in the presence of polyphenols. Altogether, molecular structure of polyphenols played a significant role in their protective effect against HSA oxidative damage and binding ability, which provided fundamental insights into their application as health care foods.


Asunto(s)
Estrés Oxidativo/efectos de los fármacos , Polifenoles/química , Polifenoles/farmacología , Albúmina Sérica Humana/metabolismo , Dicroismo Circular , Humanos , Simulación del Acoplamiento Molecular , Polifenoles/metabolismo , Unión Proteica , Conformación Proteica , Espectrometría de Fluorescencia , Termodinámica
7.
Molecules ; 26(4)2021 Feb 03.
Artículo en Inglés | MEDLINE | ID: mdl-33546142

RESUMEN

According to the World Health Organization, cardiovascular diseases are responsible for 31% of global deaths. A reduction in mortality can be achieved by promoting a healthy lifestyle, developing prevention strategies, and developing new therapies. Polyphenols are present in food and drinks such as tea, cocoa, fruits, berries, and vegetables. These compounds have strong antioxidative properties, which might have a cardioprotective effect. The aim of this paper is to examine the potential of polyphenols in cardioprotective use based on in vitro human and rat cardiomyocytes as well as fibroblast research. Based on the papers discussed in this review, polyphenols have the potential for cardioprotective use due to their multilevel points of action which include, among others, anti-inflammatory, antioxidant, antithrombotic, and vasodilatory. Polyphenols may have potential use in new and effective preventions or therapies for cardiovascular diseases, yet more clinical studies are needed.


Asunto(s)
Antioxidantes , Cardiotónicos , Enfermedades Cardiovasculares/prevención & control , Fibroblastos/metabolismo , Miocitos Cardíacos/metabolismo , Polifenoles , Animales , Antioxidantes/química , Antioxidantes/farmacología , Cardiotónicos/química , Cardiotónicos/farmacología , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/patología , Línea Celular , Fibroblastos/patología , Humanos , Miocitos Cardíacos/patología , Polifenoles/química , Polifenoles/farmacología , Ratas
8.
Molecules ; 26(3)2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-33573150

RESUMEN

Wine is one of the most consumed beverages around the world. It is composed of alcohols, sugars, acids, minerals, proteins and other compounds, such as organic acids and volatile and phenolic compounds (also called polyphenols). Polyphenols have been shown to be highly related to both (i) wine quality (color, flavor, and taste) and (ii) health-promoting properties (antioxidant and cardioprotective among others). Polyphenols can be grouped into two big families: (i) Flavonoids, including anthocyanidins, flavonols, flavanols, hydrolysable and condensed tannins, flavanones, flavones and chalcones; and (ii) Non-flavonoids, including hydroxycinnamic acids, hydroxybenzoic acids, stilbenes, tyrosol and hydroxytyrosol. Each group affects in some way the different properties of wine to a greater or a lesser extent. For that reason, the phenolic composition can be managed to obtain singular wines with specific, desirable characteristics. The current review presents a summary of the ways in which the phenolic composition of wine can be modulated, including (a) invariable factors such as variety, field management or climatic conditions; (b) pre-fermentative strategies such as maceration, thermovinification and pulsed electric field; (c) fermentative strategies such as the use of different yeasts and bacteria; and (d) post-fermentative strategies such as maceration, fining agents and aging. Finally, the different extraction methods and analytical techniques used for polyphenol detection and quantification have been also reviewed.


Asunto(s)
Antocianinas/química , Antioxidantes/química , Polifenoles/química , Vino/análisis , Alcoholes/química , Antocianinas/análisis , Flavonoides/química , Flavonoles/análisis , Flavonoles/química , Polifenoles/análisis , Gusto
9.
J Mater Chem B ; 9(6): 1478-1490, 2021 02 19.
Artículo en Inglés | MEDLINE | ID: mdl-33427844

RESUMEN

Over the past decades, near infrared light (NIR)-sensitive photothermal agents (PTAs) that can efficiently absorb light and generate heat have been investigated worldwide for cancer photothermal therapy (PTT) and the combination treatments, which have some peculiar advantages including spatiotemporal targeting, the ability-to-reverse multidrug resistance, the immunity-stimulating function, and the synergistic effect in combination treatments. In this review, we first focus on emerging melanin-like polymers and coordination polyphenol polymer-based PTAs that hold transition potential because of their facile synthesis and good biocompatibility/biodegradability. We briefly introduce polymeric PTAs for emerging NIR-II (1000-1700 nm) PTT in deep tumors to overcome shallow penetration depth and threshold irradiation intensity of NIR-I (700-900 nm). Then we discuss polymeric PTAs for combination PTT treatments with photodynamic therapy (PDT), ferroptosis therapy (ferrotherapy), and immunotherapy, which are intensively studied for achieving anticancer synergistic effects. Finally, we discuss those polymeric PTAs for reversing cancer multidrug resistance and for mild/low-temperature PTT (43 °C ≤ T < 50 °C) in contrast to conventional high-temperature PTT (>50 °C). The polymeric PTA-based PTT and the combination treatments are still being developed in the early stage and need much more effort before potential clinical transitions and applications.


Asunto(s)
Antineoplásicos/farmacología , Neoplasias/tratamiento farmacológico , Fotoquimioterapia , Fármacos Fotosensibilizantes/farmacología , Polifenoles/farmacología , Animales , Antineoplásicos/química , Supervivencia Celular/efectos de los fármacos , Humanos , Hipertermia Inducida , Neoplasias/patología , Fármacos Fotosensibilizantes/química , Polifenoles/química
10.
Food Chem ; 342: 128258, 2021 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-33508899

RESUMEN

Due to a number of unparalleled advantages such as fastness, accuracy, intactness, nuclear magnetic resonance spectroscopy (NMR) has fulfilled a significant role in determining structures and dynamics of various physical, chemical and biological systems in the field of food analysis. This study introduced the principle of NMR, key NMR techniques such as 1H NMR, DOSY, NOESY, HSQC, etc., and the knowledge of NMR applications on the evaluation of complex food system, especially the interactions of food components. The reviewed research work provides sufficient evidence that NMR spectroscopy has been an invaluable tool and will play an increasingly important role in specific technical support for food assessment. In addition, NMR combined with various other technologies could give a complete picture of the mechanism of the performance of functional food compounds, which are vital for human health and influence the intrinsic food properties during processing, storage and transportation at the molecular level.


Asunto(s)
Análisis de los Alimentos/métodos , Espectroscopía de Resonancia Magnética , Humanos , Lípidos/química , Polifenoles/química , Polisacáridos/química , Proteínas/química
11.
Food Chem ; 347: 129006, 2021 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-33472117

RESUMEN

Multilayer bottles consisting of chitosan (CS), microcrystalline cellulose (MCC), whey protein isolate (WPI), and polyethylene terephthalate (PET) were tested as novel materials for packaging and extending shelf life of rosebud beverages. We studied the storage stability at 4 °C, 25 °C, 37 °C, and 55 °C by assessing the physical and biochemical parameters. The results show that multilayer PET bottles had better barrier performance and improved soluble solids content, pH, polyphenol content, color indices, and browning degree in rosebud beverages over the control at all studied temperatures. A shelf life model was established based on the Arrhenius equation, and the number of days when polyphenol contents dropped to <50% of the initial content was defined as the shelf life. Our results highlight the reliability of the prediction model, and we conclude that packaging rosebud beverages in multilayer PET bottles significantly extends the product shelf life, and this benefit was further extended at low temperatures.


Asunto(s)
Bebidas/análisis , Celulosa/química , Quitosano/química , Embalaje de Alimentos/métodos , Tereftalatos Polietilenos/química , Proteína de Suero de Leche/química , Color , Almacenamiento de Alimentos , Concentración de Iones de Hidrógeno , Polifenoles/química , Temperatura
12.
Molecules ; 26(2)2021 Jan 06.
Artículo en Inglés | MEDLINE | ID: mdl-33419117

RESUMEN

In this study, a new method for economical utilization of coffee grounds was developed and tested. The resulting materials were characterized by proximate and elemental analyses, thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and N2 adsorption-desorption at 77 K. The experimental data show bio-oil yields reaching 42.3%. The optimal activated carbon was obtained under vacuum pyrolysis self-activation at an operating temperature of 450 °C, an activation temperature of 600 °C, an activation time of 30 min, and an impregnation ratio with phosphoric acid of 150 wt.%. Under these conditions, the yield of activated carbon reached 27.4% with a BET surface area of 1420 m2·g-1, an average pore size of 2.1 nm, a total pore volume of 0.747 cm3·g-1, and a t-Plot micropore volume of 0.428 cm3·g-1. In addition, the surface of activated carbon looked relatively rough, containing mesopores and micropores with large amounts of corrosion pits.


Asunto(s)
Carbón Orgánico/química , Café/química , Aceites Vegetales/química , Polifenoles/química , Adsorción
13.
Molecules ; 26(2)2021 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-33467101

RESUMEN

Several epidemiological studies and clinical trials have reported the beneficial effects of green tea, coffee, wine, and curry on human health, with its anti-obesity, anti-cancer, anti-diabetic, and neuroprotective properties. These effects, which have been supported using cell-based and animal studies, are mainly attributed to epigallocatechin gallate found in green tea, chlorogenic acid in coffee, resveratrol in wine, and curcumin in curry. Polyphenols are proposed to function via various mechanisms, the most important of which is related to reactive oxygen species (ROS). These polyphenols exert conflicting dual actions as anti- and pro-oxidants. Their anti-oxidative actions help scavenge ROS and downregulate nuclear factor-κB to produce favorable anti-inflammatory effects. Meanwhile, pro-oxidant actions appear to promote ROS generation leading to the activation of 5'-AMP-activated protein kinase, which modulates different enzymes and factors with health beneficial roles. Currently, it remains unclear how these polyphenols exert either pro- or anti-oxidant effects. Similarly, several human studies showed no beneficial effects of these foods, and, by extension polyphenols, on obesity. These inconsistencies may be attributed to different confounding study factors. Thus, this review provides a state-of-the-art update on these foods and their principal polyphenol components, with an assumption that it prevents obesity.


Asunto(s)
Café/química , Depuradores de Radicales Libres , Obesidad/tratamiento farmacológico , Polifenoles , Té/química , Vino , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/uso terapéutico , Humanos , Obesidad/metabolismo , Obesidad/patología , Polifenoles/química , Polifenoles/uso terapéutico
14.
J Sci Food Agric ; 101(2): 441-448, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32648586

RESUMEN

BACKGROUND: Low molecular-weight phenolic fractions (LMPFs) were extracted from Albion (LMPF-A) and Camarosa (LMPF-C) strawberry cultivars. Their antibacterial activity against Listeria monocytogenes and Salmonella Typhimurium cocktails in vitro and in vivo was investigated using strawberry juice as a food model. This study also sought to determine their antibacterial mechanism. RESULTS: Quercetin was identified as a principal compound in both phenolic fractions. The minimum bactericide concentration (MBC) values were 750 and 850 µg mL-1 (LMPF-C) and 800 and 950 µg mL-1 (LMPF-A) against S.Typhimurium and L. monocytogenes, respectively. The possible antibacterial activity of the phenolic extracts could be related to the release of phosphate and potassium ions, the effect of the disruption of membrane integrity on L. monocytogenes, and the effect of the inhibition of dihydronicotinamide adenine dinucleotide (NADH) oxidase activity on S. Typhimurium. Quercetin and kaempferol were the most active compounds in producing bacterial damage. Strawberry juice supplemented with the phenolic fractions and incubated at 37, 20, and 4 °C reduced bacterial viability; moreover, after treatment with the phenolic fraction at the lowest temperature, no viable cells were detected after 7 days' incubation. Salmonella was more sensitive to the supplements than Listeria in strawberry juice. CONCLUSIONS: This study could form the basis for the development of natural antibacterial agents that could be included in natural juice or used by the pharmaceutical industry. © 2020 Society of Chemical Industry.


Asunto(s)
Antibacterianos/farmacología , Fragaria/química , Jugos de Frutas y Vegetales/microbiología , Listeria monocytogenes/efectos de los fármacos , Viabilidad Microbiana/efectos de los fármacos , Extractos Vegetales/farmacología , Polifenoles/farmacología , Salmonella typhimurium/efectos de los fármacos , Antibacterianos/química , Frutas/química , Jugos de Frutas y Vegetales/análisis , Listeria monocytogenes/crecimiento & desarrollo , Extractos Vegetales/química , Polifenoles/química , Salmonella typhimurium/crecimiento & desarrollo
15.
Food Chem ; 338: 127724, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32795878

RESUMEN

Peppermint is widely used medicinal plant with distinguished bioactive potential, therefore, the aim of present work was to develop novel peppermint extracts with high activity by application of traditional and emerging separation techniques. Conventional hydrodistillation and microwave-assisted hydrodistillation (MWHD) were applied for recovery of essential oil (EO), while organic solvent extraction using Soxhlet apparatus, microwave-assisted and ultrasound-assisted process and supercritical fluid extraction (SFE) were applied for non-selective recovery of peppermint lipophilic extracts. Extracts were characterized in terms of terpenoids profile with special emphasis on content of major compounds (mentol, menthone, isomenthol and eucalyptol). Antioxidant activity (DPPH, ABTS, CUPRAC, FRAP, chelating and phosphomolybdenum assay) and enzyme-inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase and glucosidase inhibition) were used for screening of peppermint bioactivity. MWHD was recognized as alternative for traditional process in EO recovery, while SFE extracts were useful for green production of solvent-free peppermint extracts rich in terpenoids and other lipophilic bioactives.


Asunto(s)
Antioxidantes/química , Inhibidores Enzimáticos/química , Mentha piperita/química , Aceites Volátiles/química , Extractos Vegetales/química , Acetilcolinesterasa/química , Acetilcolinesterasa/metabolismo , Cromatografía con Fluido Supercrítico , Inhibidores Enzimáticos/metabolismo , Mentha piperita/metabolismo , Microondas , Polifenoles/química , Polifenoles/aislamiento & purificación , Análisis de Componente Principal , Sonicación , Terpenos/química , Terpenos/aislamiento & purificación
16.
Food Chem ; 338: 127535, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32798817

RESUMEN

Polyphenols are compounds naturally present in fruits and vegetables that are gaining more and more attention due to their therapeutic effects and their potential technological applications. In this review, we intend to demonstrate the importance of some phenolic compounds, addressing their biological effects and potential for applications in various industrial fields. The intake of these compounds in appropriate concentrations can present promising effects in the prevention of diseases such as diabetes, obesity, Parkinson's, Alzheimer's, and others. They can also be used to improve the physicochemical properties of starch, in the preservation of foods, as natural dyes, prebiotic ingredients, hydrogels and nanocomplexes. In addition, these compounds have potential for innovation in the most diverse technological fields, including organic fine chemistry, basic materials chemistry, pharmaceuticals, food chemistry, chemical engineering, etc.


Asunto(s)
Conservantes de Alimentos/química , Polifenoles/química , Antioxidantes/química , Enfermedades del Sistema Nervioso Central/tratamiento farmacológico , Enfermedades del Sistema Nervioso Central/patología , Colorantes/química , Humanos , Enfermedades Metabólicas/tratamiento farmacológico , Enfermedades Metabólicas/patología , Obesidad/tratamiento farmacológico , Obesidad/patología , Fenoles/química , Fenoles/uso terapéutico , Polifenoles/uso terapéutico
17.
Food Chem ; 338: 127840, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32822903

RESUMEN

In bottled wines, haze and turbidity are phenomena to be avoided. Since bentonite fining is a common process to clarify wines removing heat unstable proteins, a theoretical study on the adsorption of three Charged Model Molecules (CMMs, egg albumin, polyphenols and riboflavin) was carried out to deep comprehend this chemical phenomenon. Four bentonites were adopted and finely characterized together with the potential release of Na+ and Ca2+ cations, revealing suitable for RT albumin removal within 120 min. Better results in terms of adsorbed quantity were achieved by adopting 12%v/v EtOH/H2O solvent and by swelling bentonites for 24 h before use. With the most performing sample (Na/Ca_0.27), a comprehensive study on simultaneous adsorption of the three CMMs was performed, resulting in polyphenols adsorption increase due to their interactions with albumin. Notwithstanding the majority of albumin and riboflavin was successfully removed, ca. 40-50% of tested polyphenols was preserved.


Asunto(s)
Bentonita/química , Vino/análisis , Adsorción , Calcio/química , Calcio/metabolismo , Cromatografía Líquida de Alta Presión , Cinética , Espectrometría de Masas , Ovalbúmina/química , Polifenoles/química , Riboflavina/química , Sodio/química , Sodio/metabolismo
18.
Food Chem ; 345: 128679, 2021 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-33310256

RESUMEN

The effect of spray drying on the different polyphenolic compounds present in the root of a purple-fleshed sweet potato variety of Ipomoea batatas native from Peru was performed by HPLC-QTOF-MSMS. Nine anthocyanins, including four peonidin, three cyanidin and two pelargonidin derivatives glycosylated with sophorose and/or glucose and acylated with caffeic, ferulic and p-hydroxybenzoic acid were identified. Twenty nine cinnamoylquinic acids (CiQA), including eight mono-CiQA, fourteen di-CiQA, and five tri-CiQA, were identified on the base of their MS fragmentation profile. Relevant amounts of feruloylquinic acid derivatives were identified. Among them, some di and tri-CiQAs containing feruloyl and caffeoyl moieties in their structures, and di-feruloylquinic acids were reported here, for the first time, in Ipomoea. Spray drying process negatively affected the different phenolic groups, with polyphenol losses representing around 90% of the initial amounts. Mono-CiQAs presenting feruloyl moieties and mono acylated peonidin derivatives with p-hydroxybenzoic acid were the less affected compounds.


Asunto(s)
Desecación/métodos , Ipomoea batatas/química , Polifenoles/análisis , Ácido Quínico/análisis , Color , Polifenoles/química , Ácido Quínico/química
19.
Food Chem ; 345: 128468, 2021 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-33341300

RESUMEN

Antioxidant interactions of γ-terpinene with α-tocopherol mimic 2,2,5,7,8-pentamethyl-6-chromanol (PMHC) and caffeic acid phenethyl ester (CAPE), used as models, respectively, of mono- and poly-phenols were demonstrated by differential oximetry during the inhibited autoxidation of model substrates: stripped sunflower oil, squalene, and styrene. With all substrates, γ-terpinene acts synergistically regenerating the chain-breaking antioxidants PMHC and CAPE from their radicals, via the formation of hydroperoxyl radicals. The inhibition duration for mixtures PMHC/γ-terpinene and CAPE/γ-terpinene increased with γ-terpinene concentration, while rate constants for radical-trapping were unchanged by γ-terpinene, being 3.1 × 106 and 4.8 × 105 M-1s-1 for PMHC and CAPE in chlorobenzene (30 °C). Using 3,5-di-tert-butylcatechol and 3,5-di-tert-butyl-1,2-bezoquinone we demonstrate that γ-terpinene can reduce quinones to catechols enabling their antioxidant activity. The different synergy mechanism of γ-terpinene with mono- and poly-phenolic antioxidants is discussed and its relevance is proven in homogenous lipids using natural α-tocopherol and hydroxytyrosol as antioxidants, calling for further studies in heterogenous food products.


Asunto(s)
Monoterpenos Ciclohexánicos/química , Monoterpenos Ciclohexánicos/farmacología , Peróxidos/química , Fenoles/química , Fenoles/farmacología , Polifenoles/química , Polifenoles/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Ácidos Cafeicos/química , Ácidos Cafeicos/farmacología , Cromanos/química , Cromanos/farmacología , Sinergismo Farmacológico , Alcohol Feniletílico/análogos & derivados , Alcohol Feniletílico/química , Alcohol Feniletílico/farmacología
20.
Food Chem ; 340: 127931, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-32871358

RESUMEN

Thinned peach is abundant in polyphenols, and has been shown to exhibit various bioactivities. In this study, we evaluated the underlying immunomodulatory activity of polyphenol extracts of thinned peach (PETP) via the NF-κB and Nrf2 signaling pathways in RAW264.7 macrophages. The results demonstrated that the PETP efficiently activated the nuclear translocation of NF-κB and Nrf2, as well as downstream cytokines (IL-1ß, IL-6, TNF-α and IFN-γ), SOD activity and ROS levels in RAW264.7 cells. Specifically, the PETP of natural drying and hot air drying exhibited less efficacy than that of freeze drying in NF-κB pathway. Interestingly, the PETP of hot air drying at 50 °C was more effective than freeze-dried PETP in activating Nrf2 nuclear translocation. Additionally, 50 µg/mL PETP enhanced immune responses, whereas 800 µg/mL PETP inhibited inflammatory development in macrophages. These findings indicated that different PETP affected the immunomodulation effects differently, which associated with the drying methods and incubation concentrations.


Asunto(s)
Desecación/métodos , Factores Inmunológicos/farmacología , Factor 2 Relacionado con NF-E2/metabolismo , FN-kappa B/metabolismo , Polifenoles/farmacología , Prunus persica/química , Animales , Citocinas/metabolismo , Liofilización , Factores Inmunológicos/química , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Ratones , Polifenoles/química , Células RAW 264.7 , Transducción de Señal/efectos de los fármacos
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