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1.
Nat Commun ; 12(1): 44, 2021 01 04.
Artículo en Inglés | MEDLINE | ID: mdl-33398001

RESUMEN

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the ß2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.


Asunto(s)
Proteínas Bacterianas/metabolismo , Microbioma Gastrointestinal , Polisacáridos/metabolismo , Simbiosis , Proteínas Bacterianas/química , Microscopía por Crioelectrón , Ligandos , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Oligosacáridos/química , Polisacáridos/química , Conformación Proteica , Relación Estructura-Actividad
2.
Food Chem ; 346: 128952, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33421900

RESUMEN

Practical application of proanthocyanidins (PAs) as antioxidants is limited because of their hard-to-maintained activities during the processes and storage and in severe gastrointestinal environments. To overcome this challenge, we have developed an easy and green method to encapsulate PAs based on casein-maltodextrin Maillard conjugates. The current work entails the systematic study on the antioxidative potentials of fabricated casein-maltodextrin-PAs nanoparticles (CMPNs). In vitro antioxidant activities of CMPNs remained well during storage in 28 days and treatments under 40-80 °C. In vivo Caenorhabditis elegans (C. elegans) model further showed that the CMPNs could prolong the lifespan of nematodes and protected nematodes from oxidative stress and heat shock. Analyses of intracellular superoxide dismutase and catalase activities also confirmed the existence of an antioxidant protective effect. Besides, in vitro release test showed that the encapsulation enhanced the bioaccessibility of PAs. These results have important implications for the development of novel antioxidants in nutraceutical industries.


Asunto(s)
Antioxidantes/química , Caseínas/química , Polisacáridos/química , Proantocianidinas/química , Animales , Antioxidantes/farmacología , Caenorhabditis elegans/efectos de los fármacos , Caenorhabditis elegans/enzimología , Caenorhabditis elegans/fisiología , Catalasa/metabolismo , Longevidad/efectos de los fármacos , Reacción de Maillard , Nanopartículas/química , Estrés Oxidativo/efectos de los fármacos , Proantocianidinas/farmacología , Superóxido Dismutasa/metabolismo
3.
Nat Commun ; 12(1): 312, 2021 01 12.
Artículo en Inglés | MEDLINE | ID: mdl-33436622

RESUMEN

The transmembrane P-glycoprotein (P-gp) pumps that efflux drugs are a major mechanism of cancer drug resistance. They are also important in protecting normal tissue cells from poisonous xenobiotics and endogenous metabolites. Here, we report a fucoidan-decorated silica-carbon nano-onion (FSCNO) hybrid nanoparticle that targets tumor vasculature to specifically release P-gp inhibitor and anticancer drug into tumor cells. The tumor vasculature targeting capability of the nanoparticle is demonstrated using multiple models. Moreover, we reveal the superior light absorption property of nano-onion in the near infrared region (NIR), which enables triggered drug release from the nanoparticle at a low NIR power. The released inhibitor selectively binds to P-gp pumps and disables their function, which improves the bioavailability of anticancer drug inside the cells. Furthermore, free P-gp inhibitor significantly increases the systemic toxicity of a chemotherapy drug, which can be resolved by delivering them with FSCNO nanoparticles in combination with a short low-power NIR laser irradiation.


Asunto(s)
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Carbono/química , Sistemas de Liberación de Medicamentos , Resistencia a Antineoplásicos , Nanopartículas/química , Neoplasias/tratamiento farmacológico , Selectina-P/metabolismo , Animales , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Doxorrubicina/farmacología , Resistencia a Antineoplásicos/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Humanos , Ratones , Microfluídica , Nanopartículas/ultraestructura , Neoplasias/irrigación sanguínea , Polisacáridos/química , Dióxido de Silicio/química
4.
Food Chem ; 342: 128258, 2021 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-33508899

RESUMEN

Due to a number of unparalleled advantages such as fastness, accuracy, intactness, nuclear magnetic resonance spectroscopy (NMR) has fulfilled a significant role in determining structures and dynamics of various physical, chemical and biological systems in the field of food analysis. This study introduced the principle of NMR, key NMR techniques such as 1H NMR, DOSY, NOESY, HSQC, etc., and the knowledge of NMR applications on the evaluation of complex food system, especially the interactions of food components. The reviewed research work provides sufficient evidence that NMR spectroscopy has been an invaluable tool and will play an increasingly important role in specific technical support for food assessment. In addition, NMR combined with various other technologies could give a complete picture of the mechanism of the performance of functional food compounds, which are vital for human health and influence the intrinsic food properties during processing, storage and transportation at the molecular level.


Asunto(s)
Análisis de los Alimentos/métodos , Espectroscopía de Resonancia Magnética , Humanos , Lípidos/química , Polifenoles/química , Polisacáridos/química , Proteínas/química
5.
Anal Chem ; 93(4): 1991-2002, 2021 02 02.
Artículo en Inglés | MEDLINE | ID: mdl-33433994

RESUMEN

We developed a highly sensitive method for profiling of N-glycans released from proteins based on capillary zone electrophoresis coupled to electrospray ionization mass spectrometry (CZE-ESI-MS) and applied the technique to glycan analysis of plasma and blood-derived isolates. The combination of dopant-enriched nitrogen (DEN)-gas introduced into the nanoelectrospray microenvironment with optimized ionization, desolvation, and CZE-MS conditions improved the detection sensitivity up to ∼100-fold, as directly compared to the conventional mode of instrument operation through peak intensity measurements. Analyses without supplemental pressure increased the resolution ∼7-fold in the separation of closely related and isobaric glycans. The developed method was evaluated for qualitative and quantitative glycan profiling of three types of blood isolates: plasma, total serum immunoglobulin G (IgG), and total plasma extracellular vesicles (EVs). The comparative glycan analysis of IgG and EV isolates and total plasma was conducted for the first time and resulted in detection of >200, >400, and >500 N-glycans for injected sample amounts equivalent to <500 nL of blood. Structural CZE-MS2 analysis resulted in the identification of highly diverse glycans, assignment of α-2,6-linked sialic acids, and differentiation of positional isomers. Unmatched depth of N-glycan profiling was achieved compared to previously reported methods for the analysis of minute amounts of similar complexity blood isolates.


Asunto(s)
Electroforesis Capilar/métodos , Vesículas Extracelulares/química , Inmunoglobulina G/sangre , Espectrometría de Masas/métodos , Plasma/química , Polisacáridos/química , Sensibilidad y Especificidad
6.
Food Chem ; 337: 127798, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32799166

RESUMEN

In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents. The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs. The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP. Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs. This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.


Asunto(s)
Fraccionamiento Químico/métodos , Momordica charantia/química , Polisacáridos/aislamiento & purificación , Antioxidantes/farmacología , Polisacáridos/química , Polisacáridos/farmacología , Temperatura , Ácidos Urónicos/análisis , alfa-Amilasas/antagonistas & inhibidores , alfa-Glucosidasas/efectos de los fármacos
7.
Food Chem ; 339: 127866, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-32858386

RESUMEN

Sialylated N-glycans are an integral component of whey proteins in human milk and play an irreplaceable role in infant growth and development. Currently, there are few studies on quantitative comparison of sialylated N-glycans in milk obtained at different lactation stages. Here, a preliminary isomer-specific quantification of whey sialylated N-glycans of human colostrum milk (CM) and mature milk (MM) was performed by using our recently developed glycoqueuing strategy. Such a preliminary comparison revealed that the whey sialylated N-glycan content was 86.4% lower in MM than in CM. Twenty-three α2,6-linked sialylated N-glycan isomers were detected with no α2,3-linked isomer observed. For the first time, three mono-sialylated and four bi-sialylated glycan isomers were reported. With the prolongation of lactation, the relative abundance of mono-sialylated glycans increased, whilst the relative abundance of bi-sialylated glycans decreased significantly. These findings contribute to the understanding of the structure-function relationship of sialylated N-glycans in the human whey fraction.


Asunto(s)
Calostro/química , Glicoproteínas/química , Leche Humana/química , Ácido N-Acetilneuramínico/química , Polisacáridos/química , Análisis de Secuencia , Proteína de Suero de Leche/química , Animales , Femenino , Humanos , Isomerismo , Lactancia , Embarazo
8.
Food Chem ; 337: 128019, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32927227

RESUMEN

In this study, the nanocomplexes as a novel delivery system for curcumin, were successfully fabricated using high methoxyl pectin (HMP), individual surfactants (rhamnolipid (Rha), tea saponin (TS) and ethyl lauroyl arginate hydrochloride (ELA)) and pea protein isolate (PPI). The optimum mass ratio between PPI and curcumin was 40:1. The HMP-Rha-PPI-Cur, HMP-TS-PPI-Cur and HMP-ELA-PPI-Cur complexes which had particle sizes of 453, 422 and 587 nm, exhibited encapsulation efficiencies of curcumin with 93.46, 92.05 and 86.73%, respectively. The analysis of FTIR revealed that HMP-surfactant-PPI-Cur complexes were formed mainly by hydrogen bonding and electrostatic attraction. XRD result showed that curcumin exhibited a non-crystallized state in the ternary complexes. Moreover, the curcumin within the HMP-Rha-PPI ternary complexes showed better stability under UV-light, thermal and simulated gastrointestinal conditions.


Asunto(s)
Curcumina/administración & dosificación , Proteínas de Guisantes/química , Polisacáridos/química , Tensoactivos/química , Curcumina/química , Glucolípidos , Enlace de Hidrógeno , Modelos Biológicos , Nanopartículas/química , Tamaño de la Partícula , Pectinas/química
9.
Food Chem ; 339: 128081, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33152874

RESUMEN

In the present study, three-phase partitioning (TPP) coupled with gradient ethanol precipitation (GEP) was developed for the first time to extract and isolate polysaccharides (GPSs) from raw garlic (Allium sativum L.) bulbs. Four kinds of fructose polymers, namely, GPS35, GPS50, GPS65, and GPS80, were obtained at the final ethanol precipitation concentrations of 35%, 50%, 65%, and 80% (v/v), respectively, and their physicochemical characteristics and in vitro biological activities were investigated. Results indicated that GPS80 had higher carbohydrate (86.68% ± 0.90%) and uronic acid (12.89% ± 0.09%) contents, lower weight-average molecular weight (8.93 × 103 Da), and looser surface morphology than the other three GPSs. Furthermore, among the four GPSs, GPS80 exhibited the strongest antioxidant capacities, inhibitory effects on α-amylase and α-glycosidase, and nitric oxide stimulatory activity on RAW264.7 macrophage cells in vitro. Therefore, this study provides a simple and feasible technological strategy for producing bioactive polysaccharides from raw Allium vegetables.


Asunto(s)
Precipitación Química , Etanol/química , Ajo/química , Tallos de la Planta/química , Polisacáridos/química , Polisacáridos/farmacología , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Fenómenos Químicos , Inhibidores de Glicósido Hidrolasas/química , Inhibidores de Glicósido Hidrolasas/aislamiento & purificación , Inhibidores de Glicósido Hidrolasas/farmacología , Peso Molecular , Polisacáridos/aislamiento & purificación , alfa-Amilasas/antagonistas & inhibidores , alfa-Glucosidasas/metabolismo
10.
Food Chem ; 339: 128094, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33152882

RESUMEN

The aim of this work is to evaluate the effect of dextrose equivalent (DE) of maltodextrins (MD) on the stability of whey protein and maltodextrin stabilized oil-in-water (o/w) emulsions. Emulsions with DE 15 maltodextrin (MD 15) exhibited better stability under light acidic (pH 6), neutral and alkaline (pH 8-9) conditions, as well as during temperature ramps (20-60 °C). After 15-days of storage, MD 15 emulsion showed increase in polydispersity and decrease in the average droplet size. The apparent viscosity of the emulsions decreased with increasing DE. The shear stresses of all emulsions fitted well with the power law model (R2 > 0.9), while MD 15 showed the most stable k and n indexes. The brightness and whiteness of emulsion decreased with increases in DE. In conclusion, emulsions with MD 15 exhibited better stability, which suggests their good potential for use in the preparation of energy drinks.


Asunto(s)
Emulsiones/química , Polisacáridos/química , Proteína de Suero de Leche/química , Temperatura , Viscosidad
11.
Phytomedicine ; 80: 153356, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33039729

RESUMEN

BACKGROUND: Using natural polysaccharides from Traditional Chinese Medicine as nanodrug delivery systems have considerable potential for tumor diagnostics and therapeutics. PURPOSE: On the basis of targeted therapy and combining the advantages of natural polysaccharides (angelica polysaccharide, APS) and natural Chinese medicine (curcumin, Cur) to design functionalized nanoparticles to improve the therapeutic through cell membrane encapsulation and immunotherapy. STUDY DESIGN AND METHODS: Cur-loaded, glycyrrhetic acid (GA)-APS-disulfide bond (DTA)-Cur nanomicelle (GACS-Cur), which were prepared by the dialysis method. GACS-Cur was encapsulated with the membranes from red blood cells (RBCm) termed GACS-Cur@RBCm, which were prepared by the principle of extrusion using a miniature extruder. The developed formulations were subjected to various in vitro and in vivo evaluation tests. RESULTS: The resulting APS nanocarriers supported a favorable drug-loading capacity, biocompatibility, and enhanced synergistic anti-hepatoma effects both in vitro and in vivo. After administration in mice, in vivo imaging results showed that the GACS-Cur and RBCm-coated groups had an obvious stronger tumor tissue targeting ability than the control treatment groups. Additionally, the immunomodulatory effect increased IL-12, TNF-α and IFN-γ expression and CD8+ T cell infiltration (1.9-fold) than that of the saline group. Notably, in comparison with hyaluronic acid (HA) nanocarriers, APS nanocarriers possess higher anti-hepatoma efficiency and targeting capabilities and, thus, should be further studied for a wide range of anti-cancer applications. CONCLUSION: Our data demonstrated that APS nanocarriers encapsulated with erythrocyte membrane mighty be a promising clinical method in the development of efficacy, safety and targeting of liver cancer therapy.


Asunto(s)
Antineoplásicos Fitogénicos/administración & dosificación , Carcinoma Hepatocelular/tratamiento farmacológico , Curcumina/administración & dosificación , Neoplasias Hepáticas/tratamiento farmacológico , Polisacáridos/química , Angelica/química , Animales , Antineoplásicos Fitogénicos/inmunología , Antineoplásicos Fitogénicos/farmacología , Biomimética , Carcinoma Hepatocelular/inmunología , Carcinoma Hepatocelular/patología , Curcumina/farmacología , Portadores de Fármacos/administración & dosificación , Portadores de Fármacos/química , Sistemas de Liberación de Medicamentos/métodos , Femenino , Células Hep G2 , Humanos , Factores Inmunológicos/administración & dosificación , Factores Inmunológicos/farmacología , Neoplasias Hepáticas/inmunología , Neoplasias Hepáticas/patología , Ratones Desnudos , Micelas , Nanopartículas/administración & dosificación , Nanopartículas/química , Distribución Tisular , Ensayos Antitumor por Modelo de Xenoinjerto
12.
Biochim Biophys Acta Gen Subj ; 1865(1): 129733, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-32949621

RESUMEN

BACKGROUND: The HIV-1 envelope glycoprotein (Env) is the primary target for broadly neutralizing antibodies (bNAbs) which can block infection. The current design strategy of soluble forms of Env in native-like trimeric conformation induces neutralizing antibodies with minimal breadth and potency. Extensive shielding by N-glycans on the surface of the HIV-1 Env acts as an immune evasion mechanism by restricting B cell recognition of conserved neutralizing determinants. An alternate approach is to design Env protein with glycan deletion to expose the protein surface. METHODS: A stable native-like trimeric Env with glycan holes at potentially immunogenic locations is expected to elicit better induction of germ-line B-cells due to exposure of the immunogenic regions. However, the extent and consequences of glycan removal from the trimer apex that form an important epitope is not explored. In this work, we have designed a construct with glycans deleted from the trimer apex of an Indian clade C origin Env that has previously been characterized for immunogenicity, to understand the impact of deglycosylation on the structural and functional integrity as well as on the antibody binding properties. RESULTS: The V1V2 glycan-deleted protein maintains native-like trimeric conformation with improved accessibility of the V1V2-directed germ-line antibodies. Furthermore, we showed that the protein binds specifically to quaternary conformation-dependent bnAbs but minimally to non-neutralizing antibodies. CONCLUSIONS: This study provide an important design aspect of HIV-1 Env-based immunogens with glycan holes in the apex region that could be useful in eliciting apex directed antibodies in immunization studies.


Asunto(s)
Anticuerpos Neutralizantes/inmunología , Infecciones por VIH/virología , VIH-1/inmunología , Polisacáridos/inmunología , Productos del Gen env del Virus de la Inmunodeficiencia Humana/inmunología , Línea Celular , Epítopos/química , Epítopos/genética , Epítopos/inmunología , Infecciones por VIH/inmunología , VIH-1/química , VIH-1/genética , Humanos , Modelos Moleculares , Polisacáridos/química , Polisacáridos/genética , Conformación Proteica , Multimerización de Proteína , Estabilidad Proteica , Productos del Gen env del Virus de la Inmunodeficiencia Humana/química , Productos del Gen env del Virus de la Inmunodeficiencia Humana/genética
13.
Food Chem ; 334: 127557, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32712488

RESUMEN

Previous studies indicate that the bioactive compounds of Eugenia stipitata pulp have antimutagenic, anticarcinogenic and antigenotoxic properties, but its use has been limited due to its high perishability. The aim of this study was to preserve bioactivity by using spray-drying microencapsulation, and is pioneering for its use of DSC to determine the best proportion of wall material (maltodextrin or gum arabic) and drying temperature (100 or 120 °C). The microparticles with maltodextrin (1:9)-100 °C had the best bioactivity conservation after in vitro gastrointestinal digestion, conserving 61% of total polyphenols, and 101%, 85% and 31% of antioxidant capacity according to the ABTS, FRAP and DPPH test methods respectively. These microparticles had a spherical morphology, presented good thermal stability and can be stored at a temperature range from 20 to 40 °C without becoming sticky. Therefore, spray-drying microencapsulation together with DSC is important for preserving a high concentration of bioactive compounds.


Asunto(s)
Desecación/métodos , Eugenia/química , Industria de Procesamiento de Alimentos/métodos , Frutas/química , Aire , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Rastreo Diferencial de Calorimetría , Digestión , Composición de Medicamentos , Goma Arábiga/química , Extractos Vegetales/química , Polifenoles/química , Polifenoles/aislamiento & purificación , Polisacáridos/química
14.
Food Chem ; 335: 127645, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32738537

RESUMEN

The dried Ganoderma lucidum (GL) has been widely used for its pharmacological properties and bioactive ganoderic acids (GAs). Herein, extraction procedures combining ultra-sonication and heating were optimized using response surface methodology based on four variables (antioxidant activity, anti-diabetic activity, total GAs content, and total polysaccharide content) and principal component analysis. The extraction of freeze-dried GL at temperatures between 64.2 and 70 °C for 1.2 h maximized the antioxidant activity and GA content, whereas the polysaccharide content and anti-diabetic activity were maximized by extraction between 66.8 and 70 °C for more than 2.8 h. Heat-dried GL extracted at 50 °C for 3 h provided the greatest anti-inflammatory activity against HaCaT cells by suppressing the response to inflammation related cytokines at mRNA levels. These results suggest that extraction conditions might be a limiting factor for target-oriented investigations, and optimized extraction methods may improve the potential effect and quality of harvested GL products.


Asunto(s)
Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Fraccionamiento Químico/métodos , Hipoglucemiantes/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Reishi/química , Triterpenos/aislamiento & purificación , Antiinflamatorios/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Línea Celular , Fraccionamiento Químico/instrumentación , Humanos , Hipoglucemiantes/química , Hipoglucemiantes/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Triterpenos/química , Triterpenos/farmacología
15.
Food Chem ; 336: 127669, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32758804

RESUMEN

Curcumin was recently attracted great interest owing to its multiple bioactivities; however, the use of curcumin was hindered by its poor solubility and stability. In this study, curcumin-nisin-soy soluble polysaccharide nanoparticles (Cur-Nisin-SSPS-NPs, size = 118.76 nm) have been successfully elaborated to improve the application of curcumin. The formation of Cur-Nisin-SSPS-NPs was mediated by amphiphilic and positively charged nisin: SSPS encapsulated nisin, which was mainly driven by electrostatic attraction. And nisin-SSPS complex encapsulated curcumin mainly through hydrophobic interactions between nisin and curcumin. The encapsulation efficiency of curcumin (91.66%) in this novel nanocarriers was significantly higher than that in nanoparticles prepared by a single SSPS (31.82%) or nisin (41.69%), most likely because more hydrophobic regions of nisin were exposed after interacting with SSPS through electrostatic interaction. Consequently, this facile and green nanocarriers improved the solubility/dispersibility and stability of curcumin and nisin, as well as endowed SSPS-based nanoparticles with antioxidant and antimicrobial activities.


Asunto(s)
Curcumina/administración & dosificación , Portadores de Fármacos/química , Nanopartículas/química , Nisina/química , Polisacáridos/química , Antibacterianos/administración & dosificación , Antibacterianos/química , Antibacterianos/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Curcumina/química , Curcumina/farmacocinética , Liberación de Fármacos , Interacciones Hidrofóbicas e Hidrofílicas , Imidazoles , Pruebas de Sensibilidad Microbiana , Microscopía Electrónica de Transmisión , Morfolinas , Solubilidad , Soja/química , Espectrofotometría Ultravioleta
16.
Food Chem ; 336: 127686, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32763735

RESUMEN

The purpose of this study is to investigate the effects of the interfacial layer number on the storage stability and in vitro digestion of fish oil-loaded primary, secondary, tertiary, and quaternary multilayer emulsions stabilized by gelatin particle and polysaccharides (anionic alginate and cationic chitosan), prepared using a layer-by-layer electrostatic deposition technique. The results demonstrate that the emulsion creaming stability during the storage process and the emulsion droplet stability against the gastric phase are dependent on the interfacial layer number. But, the interfacial layer number in the multilayer emulsions has no obvious effects on the droplet stability against droplet coalescence during the storage process and against the small intestinal phases of gastrointestinal tract models. Moreover, it also has no obvious effect on the sustained free fatty acid release of multilayer emulsions. This study can advance the fundamental understanding of multilayer emulsions and promote their potential applications.


Asunto(s)
Digestión , Aceites de Pescado/química , Almacenamiento de Alimentos , Gelatina/química , Polisacáridos/química , Emulsiones , Ácidos Grasos no Esterificados/química , Aceites de Pescado/metabolismo , Tamaño de la Partícula , Electricidad Estática
17.
Food Chem ; 338: 127822, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32810813

RESUMEN

In this study, a potential of covalent linkage approach for developing active edible coatings was examined. Vanillin and trans-cinnamaldehyde were bound to chitosan by Schiff base reaction and reductive amination. The modified polysaccharides were comprehensively characterized and applied as active coatings on fresh-cut melon. The covalent linkage allowed overcoming solubility problems with the lipophilic vanillin and cinnamaldehyde and neutralizing their volatility, producing well-adhered coatings that enhanced fruit quality and storability without sensorial impairment. The attached hydrophobic moieties also provided new polysaccharides with self-assembling ability. Their aggregates were loaded with antimicrobial citral and added to mandarin juice, resulting in up to 6 log CFU/mL microbial count reduction. Thus, the covalent linkage concept offers several advantages, especially when hydrophobic or volatile active agents are used. Further developed, it may become a safe and effective tool for the formation of advanced active edible coatings and delivery vehicles for direct applications on food products.


Asunto(s)
Acroleína/análogos & derivados , Benzaldehídos/química , Películas Comestibles , Polisacáridos/química , Acroleína/química , Monoterpenos Acíclicos/química , Antiinfecciosos/química , Quitosano/química , Citrus , Cucurbitaceae/metabolismo , Microbiología de Alimentos , Conservación de Alimentos/métodos , Calidad de los Alimentos , Almacenamiento de Alimentos , Frutas/metabolismo , Jugos de Frutas y Vegetales/microbiología , Interacciones Hidrofóbicas e Hidrofílicas
18.
Food Chem ; 338: 127825, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32810814

RESUMEN

Four soluble dietary fibers (SDFs) were fortified with corn starch (CS) at different concentrations to match the same viscosity equivalents. The mixtures were subjected to a simulated digestion procedure to study the effects of SDFs on viscosity properties and digestion kinetics of CS. Results showed that SDFs increased the hydration property and decreased the water mobility of digesta. During digestion process, SDFs increased the apparent viscosity of digesta to some extent, and showed significant difference to delay the decay of digesta viscosity (kv). The amylolysis inhibitory ability was similar when each SDF was present at the same viscosity equivalent, however, significant differences were found on the digestion rate constant of k2. Linear correlations between kv and k2 were established for 1 and 2 equivalent groups. These results demonstrated that SDFs could delay the digestion process as chemistry differences, which related to their ability on delaying the change of digesta viscosity.


Asunto(s)
Fibras de la Dieta/farmacología , Almidón/química , Almidón/farmacocinética , Animales , Digestión , Jugo Gástrico/efectos de los fármacos , Jugo Gástrico/metabolismo , Cinética , Mananos/química , Polisacáridos/química , Polisacáridos Bacterianos/química , Solubilidad , Viscosidad
19.
Food Chem ; 337: 127748, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32818708

RESUMEN

In this study, pepper seed oil (PSO) was microencapsulated by spray drying at optimum conditions: oil/total solid material at 20% (w/w), gum Arabic/maltodextrin (GA/MD) at 1/5 (w/w), and air inlet temperature of 184 °C. Particle size distribution and morphology of the PSO powder (PSOP) were determined by a laser particle diameter analyzer and scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) were employed to identify the specific chemical groups of PSO, MD, and GA in the PSO-GA/MD complexes. The thermal stability of PSOP was evaluated by thermogravimetric (TGA) and differential thermal analysis (DTA). PSOP displayed inhibitory activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis although PSO had an antimicrobial activity against only Staphylococcus aureus. GA/MD microencapsulation resulted in significant preservation of PSO against oxidation during storage period.


Asunto(s)
Capsicum/química , Goma Arábiga/química , Aceites Vegetales/química , Aceites Vegetales/farmacología , Polisacáridos/química , Semillas/química , Antibacterianos/química , Antibacterianos/farmacología , Cápsulas , Desecación , Composición de Medicamentos , Estabilidad de Medicamentos , Oxidación-Reducción
20.
Food Chem ; 338: 127792, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-32827902

RESUMEN

A novel protein-based gel named "Yu dong" prepared with fish (Cyprinus carpio L.) scale aqueous extract and enhanced by polysaccharides is described in this study. The effects of pectin, alginate, and sodium carboxyl methyl cellulose (CMC-Na) on FS gel formation, stability, textural characteristics, microstructure, and water distribution were evaluated. The results indicated the viscosity of the FS gels decreased and changed slowly as the addition of pectin. While, the addition of alginate enhanced the formation of FS gels. As pectin addition in FS gels, the transition temperature decreased. When alginate and CMC-Na was added to the FS gels, the transition temperature increased. The addition of pectin, alginate, and CMC-Na to the FS gels significantly increased Gr from 44.5% to 71.99%, 61.86%, and 71.35%, respectively. Gel strength increased significantly as the addition of pectin, alginate, and CMC-Na. LF-NMR results showed that a moderate amount (0.2%) of polysaccharides bonded the protein and water more tightly, which was consistent with the SEM results showing gel structure with more uniform pores. This study provides a direct application of FS protein in preparing of gel food, which showing a better way to utilize the abandoned fish resource.


Asunto(s)
Carpas/anatomía & histología , Polisacáridos/química , Piel/química , Agua/química , Animales , Geles/química , Viscosidad
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