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1.
J Anim Sci ; 99(5)2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33939812

RESUMEN

Automatic feeding systems in pig production allow for the recording of individual feeding behavior traits, which might be influenced by the social interactions among individuals. This study fitted mixed models to estimate the direct and social effects on visit duration at the feeder of group-housed pigs. The dataset included 74,413 records of each visit duration time (min) event at the automatic feeder from 135 pigs housed in 14 pens. The sequence of visits at the feeder was employed as a proxy for the social interaction between individuals. To estimate animal effects, the direct effect was apportioned to the animal feeding (feeding pig), and the social effect was apportioned to the animal that entered the feeder immediately after the feeding pig left the feeding station (follower). The data were divided into two subsets: "non-immediate replacement" time (NIRT, N = 6,256), where the follower pig occupied the feeder at least 600 s after the feeding pig left the feeder, and "immediate replacement" time (IRT, N = 58,255), where the elapsed time between replacements was less than or equal to 60 s. The marginal posterior distribution of the parameters was obtained by Bayesian method. Using the IRT subset, the posterior mean of the proportion of variance explained by the direct effect (PrpσTemefós) was 18% for all models. The proportion of variance explained by the follower social effect (Prpσ^f2) was 2%, and the residual variance (σ^e2) decreased, suggesting an improved model fit by including the follower effect. Fitting the models with the NIRT subset, the estimate of PrpσTemefós was 20% but the Prpσ^f2 was almost zero and σ^e2 was identical for all models. For the IRT subset, the predicted best linear unbiased predictor (BLUP) of direct (Direct BLUP) and social (Follower BLUP) random effects on visit duration at the feeder of an animal was calculated. Feeder visit duration time was not correlated with traits, such as weight gain or average feed intake (P > 0.05), whereas for the daily feeder occupation time, the estimated correlation was positive with the Direct BLUP (r^ = 0.51, P < 0.05) and negative with the Follower BLUP (r^= -0.26, P < 0.05). The results suggest that the visit duration of an animal at the single-space feeder was influenced by both direct and social effects when the replacement time between visits was less than 1 min. Finally, animals that spent a longer time per day at the feeder seemed to do so by shortening the meal length of the preceding individual at the feeder.


Asunto(s)
Ingestión de Alimentos , Conducta Alimentaria , Alimentación Animal/análisis , Animales , Teorema de Bayes , Porcinos , Aumento de Peso
2.
J Anim Sci ; 99(5)2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33939813

RESUMEN

An enriched environment is widely used to improve domestic animals' welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs.


Asunto(s)
Música , Agresión , Animales , Hidrocortisona , Ruido/efectos adversos , Porcinos , Cola (estructura animal)
3.
Sci Total Environ ; 773: 145593, 2021 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-33940737

RESUMEN

Urbanisation is a global human-induced environmental change and one of the most important threats to biodiversity. To survive in human-modified environments, wildlife must adjust to the challenging selection pressures of urban areas through behaviour, morphology, physiology and/or genetic changes. Here we explore the effect of urbanisation in a large, highly adaptable and generalist urban adapter species, the wild boar (Sus scrofa, Linnaeus 1758). From 2005 to 2018, we gathered wild boar data and samples from three areas in NE Spain: one urban (Barcelona municipality, n = 445), and two non-urban (Serra de Collserola Natural Park, n = 183, and Sant Llorenç del Munt i Serra de l'Obac Natural Park, n = 54). We investigated whether urbanisation influenced wild boar body size, body mass, body condition, and the concentration of serum metabolites, considering also the effect of age, sex and use of anthropogenic food resources. Wild boars from the urban area had larger body size, higher body mass, better body condition, and a higher triglyceride and lower creatinine serum concentrations than non-urban wild boars. In addition, urban wild boars consumed food from anthropogenic origin more frequently, which suggests that differences in their diet probably induced the biometric and the metabolic changes observed. These responses are probably adaptive and suggest that wild boars are thriving in the urban environment. Our results show that urbanisation can change the morphological and physiological traits of a large mammal urban adapter, which may have consequences in the ecology and response to urban selection pressures by the species. The phenotypic plasticity shown by wild boars provides both further and new evidence on the mechanisms that allow urban adapter species of greater size to respond to urbanisation, which is expected to continue growing globally over the coming decades.


Asunto(s)
Sus scrofa , Urbanización , Animales , Animales Salvajes , Ciudades , Humanos , España , Porcinos
4.
J Am Vet Med Assoc ; 258(8): 883-891, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33825528

RESUMEN

OBJECTIVE: To evaluate and compare the anesthetic, analgesic, and cardiorespiratory effects of tiletamine-zolazepam-detomidine-butorphanol (TZDB), tiletamine-zolazepam-xylazine-butorphanol (TZXB), and ketamine-detomidine-butorphanol (KDB) in pigs and to assess anesthetic recovery duration and quality following administration of tolazoline as a reversal agent. ANIMALS: 11 healthy 2.5-month-old castrated male Landrace mixed-breed pigs. PROCEDURES: In a randomized, blinded crossover study design, pigs received the following anesthetic combinations, IM: TZDB (tiletamine-zolazepam [3 mg/kg {1.36 mg/lb}], detomidine [0.18 mg/kg {0.08 mg/lb}], and butorphanol [0.12 mg/kg {0.05 mg/lb}]); TZXB (tiletamine-zolazepam [4 mg/kg {1.8 mg/lb}], xylazine [4 mg/kg], and butorphanol [0.2 mg/kg {0.09 mg/lb}]); and KDB (ketamine [8 mg/kg {3.63 mg/lb}], detomidine [0.18 mg/kg], and butorphanol [0.3 mg/kg {0.14 mg/lb}]). A 7-day washout period was provided between treatments. At 45 minutes of anesthesia, pigs received tolazoline (2 mg/kg [0.9 mg/lb], IM; n = 6) treatment or control (5) treatment with saline (0.9% NaCl) solution. RESULTS: All anesthetic combinations induced anesthesia. Endotracheal intubation was completed within 5 minutes after anesthetic administration in all pigs, except in 2 pigs following administration of KDB. Durations (mean ± SD) of endotracheal intubation and lateral recumbency in pigs that did not receive tolazoline were 55.3 ± 4.8 minutes, 83.8 ± 15.8 minutes, and 28.2 ± 4.5 minutes and 112.4 ± 18.7 minutes, 117.2 ± 16.7 minutes, and 79.7 ± 6.0 minutes, respectively, for the TZDB, TZXB, and KDB anesthetic treatments. Tolazoline significantly shortened the duration of anesthetic recovery for all anesthetic treatments without affecting the recovery quality. CONCLUSIONS AND CLINICAL RELEVANCE: All 3 anesthetic combinations were suitable for providing anesthesia in pigs. Tolazoline administration shortened the duration of anesthetic recovery without affecting the quality of recovery.


Asunto(s)
Anestésicos , Ketamina , Anestésicos/farmacología , Animales , Butorfanol , Estudios Cruzados , Combinación de Medicamentos , Frecuencia Cardíaca , Imidazoles , Ketamina/farmacología , Masculino , Porcinos , Tiletamina/farmacología , Xilazina/farmacología , Zolazepam/farmacología
5.
Int J Mol Sci ; 22(6)2021 Mar 17.
Artículo en Inglés | MEDLINE | ID: mdl-33802769

RESUMEN

Ibuprofen is a nonsteroidal anti-inflammatory drug (NSAID) introduced in the 1960s and widely used as an analgesic, anti-inflammatory, and antipyretic. In its acid form, the solubility of 21 mg/L greatly limits its bioavailability. Since the bioavailability of a drug product plays a critical role in the design of oral administration dosage, this study investigated the enzymatic esterification of ibuprofen as a strategy for hydrophilization. This work proposes an enzymatic strategy for the covalent attack of highly hydrophilic molecules using acidic functions of commercially available bioactive compounds. The poorly water-soluble drug ibuprofen was esterified in a hexane/water biphasic system by direct esterification with sorbitol using the cheap biocatalyst porcine pancreas lipase (PPL), which demonstrated itself to be a suitable enzyme for the effective production of the IBU-sorbitol ester. This work reports the optimization of the esterification reaction.


Asunto(s)
Biocatálisis , Medios de Cultivo/química , Ibuprofeno/química , Sorbitol/química , Animales , Esterificación , Hidrólisis , Ibuprofeno/síntesis química , Lipasa/metabolismo , Solventes , Especificidad por Sustrato , Porcinos , Temperatura , Factores de Tiempo , Agua/química
7.
Medicina (B Aires) ; 81(2): 297-300, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33906152

RESUMEN

Contact electrical burns are more severe than other forms of contact burn injury. Moreover, treatment of hand burns is an important therapeutic challenge. We present a 17 year-old female of low voltage electric hand injury, admitted 5 days after injury. The patient was treated with autologous platelet rich plasma, porcine dermis heterograft and partial autologous skin graft, all of them with hyperbaric oxygen therapy (HBOT) at 1.45 ATA "100% O2 like adjuvant therapy. Good evolution and acceptable aesthetic results were reported. Although more studies are required, we suggest that multi-therapeutic approach could be effective in treatment for electric burns in hands.


Asunto(s)
Quemaduras por Electricidad , Quemaduras , Oxigenación Hiperbárica , Plasma Rico en Plaquetas , Adolescente , Animales , Quemaduras/terapia , Quemaduras por Electricidad/terapia , Femenino , Humanos , Trasplante de Piel , Porcinos
8.
J Anim Sci ; 99(4)2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33861853

RESUMEN

Previous research indicated that phytase may release less phosphorus (P) from phytate when it is evaluated using diets with P levels above requirement as compared with diets below requirement. The objectives of this experiment were to further test the hypothesis that the P release values determined for phytase are higher when pigs are fed diets that are deficient (DE) in P compared with when they are fed diets that are adequate (AD) in P, and that phytase will increase the digestibility of dry matter (DM), gross energy (GE), nitrogen (N), and calcium (Ca) independent of dietary P status. Twenty-four barrows (body weight: 23.2 ± 1.8 kg) were randomly assigned to one of eight dietary treatments and housed in individual pens for 21 d and then moved to metabolism crates for 9 d, with the collection of urine and feces occurring on the final 5 d. A basal corn-soybean meal diet (P-AD) was formulated at 0.36% standardized total tract digestible (STTD) P and total calcium:STTD P (Ca:STTD P) of 2:1. A P-DE diet was also formulated to maintain a constant Ca:STTD P of 2:1 in both basal diets. Phytase was added to AD and DE diets at 350, 600, 1,000 phytase units (FYT)/kg. Pig was the experimental unit; diet (P-AD or P-DE), phytase level, and replicate were fixed effects. Orthogonal polynomial contrasts were used to test linear and quadratic effects of phytase within P-AD and P-DE diets. Phytase improved apparent total tract digestibility (ATTD) and STTD of P in both P-AD (linear P < 0.001) and P-DE diets (quadratic P < 0.001). Estimates for STTD P release were 0.07%, 0.09%, and 0.09% for 350, 600, and 1,000 phytase units (FYT)/kg in P-DE diets, and 0.02%, 0.03%, and 0.05% in P-AD diets, respectively. In P-DE diets, phytase improved absorption and retention of P and increased urinary excretion of P (quadratic P < 0.001). In P-AD diets, phytase improved absorption of P (linear P = 0.066), tended to improve retention (linear P = 0.066), and increased urinary excretion of P (quadratic P = 0.021). Phytase improved ATTD of Ca in P-DE diets (quadratic P = 0.002) but not in P-AD diets (P > 0.1). In conclusion, the release of P by phytase is lower in diets that are AD in P than those which are DE. Phytase increased the availability of Ca only in the diets DE in P. Finally, phytase increased the ATTD of DM and tended to increase the ATTD of energy, independent of dietary P status.


Asunto(s)
6-Fitasa , Fósforo Dietético , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Digestión , Tracto Gastrointestinal , Fósforo , Soja , Porcinos , Zea mays
9.
Sheng Li Xue Bao ; 73(2): 244-252, 2021 Apr 25.
Artículo en Chino | MEDLINE | ID: mdl-33903886

RESUMEN

The aim of this study was to investigate the effects of polarization program on the ability of macrophages to regulate iron metabolism. M1 and M2 macrophages were propagated in vitro from porcine alveolar macrophages 3D4/2 and polarized by cytokines. The 3D4/2 macrophages were treated with 20 ng/mL interferon gamma (IFN-γ) and 10 ng/mL interleukin-4 (IL-4) combined with 10 ng/mL macrophage colony-stimulating factor (M-CSF) to induce polarization to M1 and M2, respectively. After incubation for 24 h, the expression levels of inflammatory factors and iron-metabolism genes were determined using real-time qPCR, Western bot and immunofluorescence. The M1/M2 macrophages culture media supernatant was collected and used to treat porcine intestinal epithelial cells IPEC-J2. The proliferation ability of IPEC-J2 was detected using CCK-8 assay kit. Following exogenous addition of ammonium ferric citrate (FAC) to M1/M2 macrophages, the phagocytic function of macrophages was detected using fluorescein isothiocyanate-dextran (FITC-dextran) and flow cytometry. The results showed that, compared with control, M1 macrophages had higher mRNA levels of iron storage proteins (ferritin heavy and light polypeptide, i.e. FtH and FtL), hepcidin and lipocalin-2, as well as iron content. Moreover, iron enhanced the ability of M1 macrophages to phagocytize FITC-dextran. There was no significant change in these mRNA expression levels in M2 macrophages, but the mRNA expression levels of ferroportin and transferrin receptor were up-regulated. In addition, the conditioned media supernatant from M2 macrophages promoted cell proliferation of IPEC-J2. These findings indicate that M1 macrophages tend to lock iron in the cell and reduce extracellular iron content, thereby inhibiting the proliferation of extracellular bacteria. While M2 macrophages tend to excrete iron, which contributes to the proliferation of surrounding cells and thus promotes tissue repair.


Asunto(s)
Citocinas , Macrófagos , Animales , Ferritinas , Hierro/metabolismo , Macrófagos/metabolismo , Macrófagos Alveolares/metabolismo , Porcinos
10.
BMC Oral Health ; 21(1): 177, 2021 04 07.
Artículo en Inglés | MEDLINE | ID: mdl-33827540

RESUMEN

BACKGROUND: We established an in vivo intraradicular biofilm model of apical periodontitis in pigs in which we compared the efficacy of different irrigant activation techniques for biofilm removal. METHODS: Twenty roots from the deciduous mandibular second premolar of 5 male pigs were used. After pulpectomy, canals were left open for 2 weeks and then sealed for 4 weeks to enable the development of an intracanal biofilm. The intraradicular biofilms was evaluated using SEM and bacterial 16S rRNA gene-sequencing. To investigate the efficacy of biofilm removal, root canal irrigations were performed using conventional needle, passive ultrasonic, subsonic, or laser-activated irrigation. Real-time PCR was conducted to quantitate the remaining biofilm components. Statistical analysis was performed using ANOVA followed by a Tukey kramer post-hoc test with α = 0.05. RESULTS: The pulp exposure model was effective in inducing apical periodontitis and SEM analysis revealed a multi-layer biofilm formation inside the root canal. 16S rRNA sequence analysis identified Firmicutes, Bacteroidetes, and Fusobacteria as the predominant bacterial phyla components, which is similar to the microbiome profile seen in humans. None of the tested irrigation techniques completely eradicated the biofilm components from the root canal, but the subsonic and laser-activated irrigation methods produced the lowest bacterial counts (p < 0.05). CONCLUSIONS: An experimental intraradicular biofilm model has been successfully established in pigs. Within the limitations of the study, subsonic or laser-activated irrigation demonstrated the best biofilm removal results in the pig system.


Asunto(s)
Cavidad Pulpar , Irrigantes del Conducto Radicular , Animales , Biopelículas , Masculino , ARN Ribosómico 16S/genética , Preparación del Conducto Radicular , Hipoclorito de Sodio , Porcinos , Irrigación Terapéutica
11.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 38(1): 111-121, 2021 Feb 25.
Artículo en Chino | MEDLINE | ID: mdl-33899435

RESUMEN

The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA in vitro to avoid wasting time and resource costs before animal experiments. In addition, how to efficiently obtain positive gene editing monoclonal cells is a difficult problem to be solved. In this study, a rapid sgRNA screening method targeting the pig genome was established and we rapidly obtained Fah gene edited cells, laying a foundation for the subsequent production of Fah knockout pigs as human hepatocyte bioreactor. At the same time, the method of obtaining monoclonal cells using pattern microarray culture technology was explored.


Asunto(s)
Sistemas CRISPR-Cas , ARN Guia , Animales , Sistemas CRISPR-Cas/genética , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas/genética , Edición Génica , ARN Guia/genética , Porcinos
12.
Sensors (Basel) ; 21(5)2021 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-33801224

RESUMEN

Measurements of ammonia with inexpensive and reliable sensors are necessary to obtain information about e.g., ammonia emissions. The concentration information is needed for mitigation technologies and documentation of existing technologies in agriculture. A flow-based fluorescence sensor to measure ammonia gas was developed. The automated sensor is robust, flexible and made from inexpensive components. Ammonia is transferred to water in a miniaturized scrubber with high transfer efficiency (>99%) and reacts with o-phthalaldehyde and sulfite (pH 11) to form a fluorescent adduct, which is detected with a photodiode. Laboratory calibrations with standard gas show good linearity over a dynamic range from 0.03 to 14 ppm, and the detection limit of the analyzer based on three-times the standard deviation of blank noise was approximately 10 ppb. The sampling frequency is 0.1 to 10 s, which can easily be changed through serial commands along with UV LED current and filter length. Parallel measurements with a cavity ring-down spectroscopy analyzer in a pig house show good agreement (R2 = 0.99). The fluorescence sensor has the potential to provide ammonia gas measurements in an agricultural environment with high time resolution and linearity over a broad range of concentrations.


Asunto(s)
Amoníaco , Ganado , Agricultura , Animales , Análisis Espectral , Porcinos
13.
Environ Sci Technol ; 55(8): 4440-4451, 2021 04 20.
Artículo en Inglés | MEDLINE | ID: mdl-33793238

RESUMEN

Pig production contributes considerably to land use and greenhouse gas (GHG) and reactive nitrogen (Nr) emissions. Land use strategies were widely proposed, but the spillover effects on biological flow are rarely explored. Here, we simultaneously assessed the carbon (C), nitrogen (N), and cropland footprints of China's pig production at the provincial scale in 2017. The environmental impacts of land use strategies were further evaluated. Results show that one kg live-weight pig production generated an average of 1.9 kg CO2-equiv and 59 g Nr emissions, occupying 3.5 m2 cropland, with large regional variations. A large reduction in GHG (58-64%) and Nr (12-14%) losses and occupied cropland (10-11%) could be achieved simultaneously if combined strategies of intensive crop production, improved feed-protein utilization efficiency, and feeding co-products were implemented. However, adopting a single strategy may have environmental side-effects. Reallocating cropland that pigs used for feed to plant food alternatives would enhance human-edible energy (3-20 times) and protein delivery (1-5 times) and reduce C and N footprints, except for rice and vegetables. Reallocating cropland to beef and milk production would decrease energy and protein supply. Therefore, a proper combination of land use strategies is essential to alleviate land use changes and nutrient emissions without sacrificing food supply.


Asunto(s)
Efecto Invernadero , Gases de Efecto Invernadero , Animales , Huella de Carbono , Bovinos , China , Gases de Efecto Invernadero/análisis , Nitrógeno/análisis , Porcinos
14.
Wiad Lek ; 74(3 cz 1): 418-422, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33813443

RESUMEN

OBJECTIVE: The aim is to develop a method of the acellular dermal matrix manufacturing from pig's skin dermis while preserving the native structure. PATIENTS AND METHODS: Materials and methods: Combination of physical and chemical effects on the dermis underlies in the process of an acellular dermal matrix manufacturing. Dermal collection of 1.0-1.3 mm thickness in pigs under 1 year of age from the back and partially from the lateral parts of the body was carried out. The 0.3-0.4 mm thickness layer of skin was previously removed from the relevant areas with help of a dermatome, which was physically and chemically treated. The maximum acellularization of the dermal matrix was achieved step-by-step and included four stages of skin processing: 1 - freeze-thaw process; 2 - glycerin dehydration; 3 - osmotic stress; 4 - cell residue removal by detergent. RESULTS: Results: Histological analysis of the of the pig's skin dermis revealed that after freeze-thaw cycles the collagen scaffold of the dermal matrix maintains its structural organization that was obtained as a result of the first stage of decellularization. On the second stage of decullalarization, the decreased number of fibroblastic cells was indicated. By means of this, the connective tissue elements that are represented by collagen fibers' multidirectional bundles retained their structural organization. Fibroblasts lysis as basophilic stained elements was revealed in small amount of dermis on the third stage of the decellularization. Washing of lyophilized skin with nonionic detergent sodium dodecyl sulfate the complete absence of fibroblasts, epidermocytes in the hair follicles, endothelial cells in the wall of blood vessels was detected indicating the effectiveness of this reagent in removing residual products. CONCLUSION: Conclusions: Suggested protocol for decullalarization of the pig's skin dermis is effective in removing nuclear and cellular structures from dermis. Particular protocols can be modified by increasing the temperature difference or changing the number of freeze-thaw cycles.


Asunto(s)
Dermis Acelular , Animales , Colágeno , Células Endoteliales , Trasplante de Piel , Porcinos
15.
Georgian Med News ; (311): 27-32, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33814385

RESUMEN

Using the mesh for hernia repair is the most common type of hernia surgery. There are many types of meshes made of various synthetic materials, but all of these meshes have their own respective disadvantages. The aim of this study was to provide preliminary results of a non-randomized clinical trial evaluation of novel porcine grafts XI-S+® (Colorado Therapeutics LLC. USA) for ventral and inguinal hernia repair. All patients underwent a standardized surgical procedure. Onlay surgical repair technique has been performed in ten patients with ventral hernia and Lichtenstein tension-free method has been used for ten patients with inguinal hernia repair. The XI-S+® mesh fixation was performed with multiple simple interrupted sutures using prolene thread. The average age of the patients with ventral hernia was 54±14 years, and 30% of patients were female and 70% of patients were male. The average age of the patients with inguinal hernia was 62.5±9.4 years, and 10% of patients were female and 90% of patients were male. The average hospitalization length was 2 days. During three years of observation, no recurrence of hernia was observed in patients. The XI-S + ® mesh has anti-adhesive properties, is extremely resistant to infections, provides favorable conditions for engraftment, early activity and patient rehabilitation. The clinical studies of the patients that underwent ventral and inguinal hernia repair using XI-S+® mesh have shown that the post-operative pain was minimal and easily controlled by the use of analgesics. As for the sensation of the mesh, in some patients it has been present up until 1 month from surgery, but it fully disappeared by the end of the 3rd month.


Asunto(s)
Hernia Inguinal , Adulto , Anciano , Animales , Materiales Biocompatibles/uso terapéutico , Femenino , Hernia Inguinal/cirugía , Herniorrafia , Humanos , Masculino , Persona de Mediana Edad , Polipropilenos , Recurrencia , Mallas Quirúrgicas , Porcinos , Resultado del Tratamiento
16.
Int J Nanomedicine ; 16: 2477-2486, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33824586

RESUMEN

Purpose: Sensitive and selective point-of-care biosensor is an urgent pursuit of serological antibody detection to control parasite pathogen. For specific, quantitative and on-site screening of Trichinella spiralis infection in livestock, a quantum dot nanobead-monoclonal antibody (QB-mAb) probe-based immunochromatographic assay (ICA) was developed by introducing a competitive sandwich strategy (QB-CICA). Methods: In the QB-CICA, QB-mAb probes competed with serum antibody for a particular epitope, followed by immunocomplexes binding to capture antibody on the test line. With the accumulation of target antibody, captured probes served as signal elements for fluorescent readout in a "turn off" mode, along with the fluorescence gradually weakened. The sensitivity and standard calibration curve of the QB-CICA were quantified using swine sera as negative control (n = 200) and artificial infected swine sera (n = 80) compared with a commercial ELISA kit. Besides, Trichinella spiralis-antibody targeting test ability of the QB-CICA, instead of other parasites or viruses antibodies (n = 10), was evaluated. Results: The QB-CICA exhibited a good linear range, a low detection limit of 189.92 ng mL-1 and 100% selectivity that was higher than commercial ELISA kit (90%), as well as the same serological positive rate (100%) with commercial ELISA kit in different infection dose models. Conclusion: Taking advantage of its simplicity, short response time (25 min), sensitivity and specificity, the proposed QB-CICA has potential applications for parasite-related antibody monitoring in food safety and clinical diagnosis fields.


Asunto(s)
Anticuerpos Antihelmínticos/análisis , Anticuerpos Monoclonales/inmunología , Cromatografía de Afinidad/métodos , Nanopartículas/química , Puntos Cuánticos/química , Trichinella spiralis/inmunología , Triquinelosis/diagnóstico , Triquinelosis/inmunología , Animales , Anticuerpos Antihelmínticos/sangre , Anticuerpos Antihelmínticos/inmunología , Nanopartículas/ultraestructura , Puntos Cuánticos/ultraestructura , Porcinos , Triquinelosis/parasitología
17.
Sensors (Basel) ; 21(9)2021 Apr 21.
Artículo en Inglés | MEDLINE | ID: mdl-33919359

RESUMEN

The voice producing process is a complex interplay between glottal pressure, vocal folds, their elasticity and tension. The material properties of vocal folds are still insufficiently studied, because the determination of material properties in soft tissues is often difficult and connected to extensive experimental setups. To shed light on this less researched area, in this work, a dynamic pipette aspiration technique is utilized to measure the elasticity in a frequency range of 100-1000 Hz. The complex elasticity could be assessed with the phase shift between exciting pressure and tissue movement. The dynamic pipette aspiration setup has been miniaturized with regard to a future in-vivo application. The techniques were applied on 3 different porcine larynges 4 h and 1 d postmortem, in order to investigate the deterioration of the tissue over time and analyze correlation in elasticity values between vocal fold pairs. It was found that vocal fold pairs do have different absolute elasticity values but similar trends. This leads to the assumption that those trends are more important for phonation than having same absolute values.


Asunto(s)
Laringe , Pliegues Vocales , Animales , Fenómenos Biomecánicos , Elasticidad , Fonación , Porcinos
18.
J Chromatogr A ; 1644: 462083, 2021 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-33819677

RESUMEN

Meat heating endpoint temperature (EPT) is an important indicator to ensure the safety of cooked meat. Accurately determining the EPT of cooked meat and ready-to-eat meat products is an important strategy to ensure food safety. In this study, a comprehensive metabolic method based on UPLC-Q Exactive and chemometrics was developed to study the metabolites differences among pork roasted at different temperatures in order to select markers indicating EPT and discover new toxic heat-induced compounds. A two-step extraction method was applied to avoid the loss of metabolite information caused by sample preparation. Using chemometrics, the five compounds of creatine, creatinine, 2-amino-1-methyl-6-phenylimidazo (4,5-b) pyridine (PhIP), 2-methyl-6-amino-5-hydroxymethylpyrimidine (TMP) and compound with the m/z of 114.04316 were selected as markers, and four of them were further confirmed by chemical standards. It is worth noting that TMP was discovered in roasted pork for the first time. In addition, targeting studies aimed at quantifying the selected markers were conducted at different thermal processing temperatures. From the quantification results, it can be concluded that the heat temperature not exceed 180 °C is recommended to reduce the content of toxic compounds. This study has proved that the integration of UPLC-Q Exactive and chemometrics could provide an efficient method for the study of markers related to thermal process and new toxic heat-induced compounds.


Asunto(s)
Espectrometría de Masas/métodos , Carne de Cerdo/análisis , Temperatura , Animales , Análisis Discriminante , Imidazoles/análisis , Análisis de los Mínimos Cuadrados , Metabolómica , Análisis Multivariante , Análisis de Componente Principal , Estándares de Referencia , Estadística como Asunto , Porcinos
19.
J Zoo Wildl Med ; 52(1): 343-347, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33827197

RESUMEN

Pygmy hogs (Porcula salvania) are the smallest and rarest wild suid. It is categorized as a Critically Endangered species as per the Red List of the International Union for Conservation of Nature. This study reports the first detection of a single-stranded RNA virus species, Aichivirus C, belonging to the genus Kobuvirus (KobV) and the family Picornaviridae, in pygmy hogs. KobV species are identified as a cause of acute gastroenteritis among children in India. As of now, there exists no report on the detection of KobV in animals from India. We used a detection assay based on reverse transcription-polymerase chain reaction for KobV screening in pygmy hogs from a conservation center in India. The 3D polymerase gene-based molecular analysis revealed KobV presence in the Indian wild suid, pygmy hogs. Of the 15 samples tested, three were found positive for picornaviruses and were negative for rotavirus A, rotavirus C, astrovirus, picobirnavirus and caliciviruses. Nucleotide-based sequence analysis of the partial 3D polymerase gene revealed close identity with porcine KobV from the Czech Republic (JX232619, 90.6%-91.6%) and Hungary (NC_011829, 89.8%-91.6%), wherein one of the current study strains clustered with the Czech Republic JX232619 strain in the phylogenetic tree. Further investigation of the role of KobV in health and disease of pygmy hogs is warranted.


Asunto(s)
Especies en Peligro de Extinción , Kobuvirus/aislamiento & purificación , Porcinos/virología , Animales , Heces/virología , Femenino , India/epidemiología , Masculino
20.
Bioresour Technol ; 332: 125039, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-33845317

RESUMEN

Rice straw (RS) is one of abundant agricultural waste for biogas production in China. However, the low carbon-methane conversion rate limits its wide application due to the low degradation rate of RS during fermentation. This study investigated the effect of acclimatized paddy soil microorganisms using swine wastewater on degradation of RS before anaerobic digestion. The total organic carbon, reducing sugar and NH4+-N content of paddy soil + RS + swine wastewater (PRS) (653.50 mg/L) was higher than that of other groups after 19 days. The carboxymethyl cellulose activity (4.01 IU), cellulose/lignin ratio (5.25) and the degradation rate of lignin (51.96%) in PRS were higher than those of other groups. The Firmicutes (21.02%), Chloroflexi (12.48%), Proteobacteria (20.92%), and Bacteroidetes (25.78%) were the main fermentation phyla in PRS during acclimatization. These results indicated that the acclimatized paddy soil microorganisms using swine wastewater (SW) could degrade RS more efficiently.


Asunto(s)
Oryza , Suelo , Animales , China , Metano , Porcinos , Aguas Residuales
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