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1.
Anticancer Res ; 41(4): 2083-2092, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33813418

RESUMEN

BACKGROUND/AIM: Numerical aberrations of permanent dentition and dystopic tooth eruption are part of the phenotype of the tumor predisposition syndrome neurofibromatosis type 1 (NF1). In these cases, surplus tooth germs usually develop in the alveolar processes of the jaw. This report attests to the dystopic development of a dysplastic supernumerary tooth in NF1 arising outside the jaw. CASE REPORT: The 8-year-old male patient developed a microdont outside the bone and above the occlusal plane of the retained maxillary right second molar. The supernumerary tooth was completely embedded in oral soft tissue. Hyperplastic oral soft tissue in the molar region and microdont were excised. Specimen of the mucosa surrounding the teeth was interspersed with diffuse and plexiform neurofibroma. The retained upper right first molar emerged spontaneously within a few months after surgery. The upper right second molar did not change position. CONCLUSION: Odontogenesis can take place within tumorous oral mucosa in NF1. Surgical removal of the tumorous mucous membrane facilitates tooth eruption in some cases.


Asunto(s)
Proceso Alveolar/patología , Neoplasias de la Boca/diagnóstico , Neurofibroma Plexiforme/diagnóstico , Neurofibromatosis 1/diagnóstico , Erupción Ectópica de Dientes/diagnóstico , Proceso Alveolar/anomalías , Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/cirugía , Niño , Humanos , Masculino , Neoplasias de la Boca/complicaciones , Neoplasias de la Boca/patología , Neoplasias de la Boca/cirugía , Neurofibroma Plexiforme/complicaciones , Neurofibroma Plexiforme/patología , Neurofibroma Plexiforme/cirugía , Neurofibromatosis 1/complicaciones , Neurofibromatosis 1/patología , Neurofibromatosis 1/cirugía , Anomalías Dentarias/diagnóstico , Anomalías Dentarias/etiología , Anomalías Dentarias/patología , Anomalías Dentarias/cirugía , Erupción Ectópica de Dientes/etiología , Erupción Ectópica de Dientes/cirugía , Diente Primario/anomalías , Diente Primario/diagnóstico por imagen , Diente Primario/patología , Diente Primario/cirugía , Diente Supernumerario/diagnóstico , Diente Supernumerario/etiología , Diente Supernumerario/patología , Diente Supernumerario/cirugía
2.
PLoS One ; 15(8): e0237660, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32841254

RESUMEN

This study evaluated the influence of type 2 diabetes mellitus on bone loss, bone repair and cytokine production in hyperglycemic rats, treated or not with metformin. The animals were distributed as follow: Non-Hyperglycemic (NH), Non Hyperglycemic with Ligature (NH-L), Treated Non Hyperglycemic (TNH), Treated Non Hyperglycemic with Ligature Treated (TNH-L), Hyperglycemic (H), Treated Hyperglycemic (TH), Hyperglycemic with Ligature (H-L), Treated Hyperglycemic with Ligature (TH-L). At 40th day after induction of hyperglycemia, the groups NH-L, TNH-L, H-L, TH-L received a ligature to induce periodontitis. On the 69th, the TNH, TNH-L, TH, TH-L groups received metformin until the end of the study. Bone repair was evaluated at histometric and the expression levels of Sox9, RunX2 and Osterix. Analysis of the ex-vivo expression of TNF-α, IFN-γ, IL-12, IL-4, TGF-ß, IL-10, IL-6 and IL-17 were also evaluated. Metformin partially reverse induced bone loss in NH and H animals. Lower OPG/RANKL, increased OCN and TRAP expression were observed in hyperglycemic animals, and treatment with metformin partially reversed hyperglycemia on the OPG/RANKL, OPN and TRAP expression in the periodontitis. The expression of SOX9 and RunX2 were also decreased by hyperglycemia and metformin treatment. Increased ex vivo levels of TNF-α, IL-6, IL-4, IL-10 and IL-17 was observed. Hyperglycemia promoted increased IL-10 levels compared to non-hyperglycemic ones. Treatment of NH with metformin was able to mediate increased levels of TNF-α, IL-10 and IL-17, whereas for H an increase of TNF-α and IL-17 was detected in the 24- or 48-hour after stimulation with LPS. Ligature was able to induce increased levels of TNF-α and IL-17 in both NH and H. This study revealed the negative impact of hyperglycemia and/or treatment with metformin in the bone repair via inhibition of transcription factors associated with osteoblastic differentiation.


Asunto(s)
Pérdida de Hueso Alveolar/prevención & control , Diabetes Mellitus Experimental/complicaciones , Diabetes Mellitus Tipo 2/complicaciones , Hiperglucemia/complicaciones , Metformina/administración & dosificación , Periodontitis/prevención & control , Pérdida de Hueso Alveolar/etiología , Pérdida de Hueso Alveolar/metabolismo , Proceso Alveolar/citología , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/metabolismo , Proceso Alveolar/patología , Animales , Regeneración Ósea/efectos de los fármacos , Regeneración Ósea/genética , Diferenciación Celular/efectos de los fármacos , Citocinas/metabolismo , Diabetes Mellitus Experimental/inducido químicamente , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Tipo 2/inducido químicamente , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Humanos , Hiperglucemia/inducido químicamente , Hiperglucemia/tratamiento farmacológico , Hiperglucemia/metabolismo , Osteoblastos/fisiología , Periodontitis/etiología , Periodontitis/metabolismo , Ratas , Estreptozocina/toxicidad , Factores de Transcripción/metabolismo
3.
Cell Prolif ; 53(10): e12886, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32794619

RESUMEN

OBJECTIVES: Diabetes aggravates the risk and severity of periodontitis, but the specific mechanism remains confused. Complement 3 (C3) is closely related to complications of type 2 diabetes (T2DM). In the present study, we concentrated on whether C3 mediates the development of periodontitis in T2DM. MATERIALS AND METHODS: Levels of C3 in blood and gingival crevicular fluid (GCF) of patients were measured first. A C3-knockout diabetic mouse model was established, real-time PCR, Western blotting and histological investigation were performed to evaluate the progress of periodontitis. Microcomputed tomography (micro-CT) and TRAP staining were performed to detect alveolar bone resorption. Immunofluorescence was performed to detect polarization of macrophages. RESULTS: Our data showed that C3 levels were elevated in the blood and GCF of T2DM patients compared with non-diabetic individuals. Increased C3 was closely related to the upregulation of inflammatory cytokines including interleukin (IL)-1, IL-6 and tumour necrosis factor-alpha (TNF-α), as well as the decline of the bone volume density (BMD) and bone volume over total volume (BV/TV) of the alveolar bones in diabetic mice. The deletion of C3 inhibited inflammatory cytokines and rescued the decreased BMD and BV/TV of the alveolar bones. C3-mediated polarization of macrophages was responsible for the damage. CONCLUSION: T2DM-related upregulation of C3 contributes to the development of periodontitis by promoting macrophages M1 polarization and inhibiting M2 polarization, triggering a pro-inflammatory effect on periodontal tissues.


Asunto(s)
Complemento C3/metabolismo , Diabetes Mellitus Tipo 2/patología , Macrófagos/inmunología , Periodontitis/diagnóstico , Adulto , Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/patología , Proceso Alveolar/fisiología , Animales , Densidad Ósea , Complemento C3/análisis , Complemento C3/genética , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patología , Diabetes Mellitus Tipo 2/complicaciones , Femenino , Líquido del Surco Gingival/metabolismo , Humanos , Interleucina-6/genética , Interleucina-6/metabolismo , Activación de Macrófagos , Macrófagos/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Persona de Mediana Edad , Periodontitis/etiología , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo
4.
BMC Oral Health ; 20(1): 195, 2020 07 08.
Artículo en Inglés | MEDLINE | ID: mdl-32641041

RESUMEN

BACKGROUND: It is not well-known which pre-implantological procedures are preferred by maxillofacial (MFS) and oral surgeons (OS) for the narrow atrophic alveolar ridge under practice based conditions and, if different training paths in surgery lead to other pre-implantological techniques being preferred. This study aims to identify which procedures are preferred by the respective specialists in which indication. METHODS: A questionnaire was sent to a total of 300 MFS and OS in southern Germany. The questionnaire examined pre-implantological procedures (bone block, bone grafting material and/or particulate autogenous bone, titanium mesh, bone split, resection) in the edentulous severely atrophic mandible and in the severely atrophic single-tooth gap. Kendall's Tau-b test was used for statistical analyses. RESULTS: One hundred seventeen participants returned the questionnaire. 68 (58%) were OS and 49 (42%) were MFS. In the edentulous mandible, bone substitute material and resection were most preferred by both specialists. Bone blocks were statistically significantly more frequently associated with MFS and bone substitute materials with OS. Bone split was more frequently used in the atrophic single tooth gap than in the edentulous mandible. OS preferred bone blocks in the single tooth gap more often than in the edentulous mandible. MFS and OS preferred resection in the edentulous mandible significantly more frequently than in the single tooth gap. CONCLUSIONS: MFS in general prefer more invasive pre-implantological therapies with the same initial diagnosis than OS, which seems to be attributed to different training paths.


Asunto(s)
Aumento de la Cresta Alveolar , Implantes Dentales , Cirujanos Oromaxilofaciales/psicología , Pautas de la Práctica en Medicina , Proceso Alveolar/patología , Atrofia/patología , Trasplante Óseo , Implantación Dental Endoósea , Alemania , Humanos , Masculino , Mandíbula/patología
5.
J Pathol ; 251(3): 323-335, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-32418202

RESUMEN

The lymphatic system plays a crucial role in the maintenance of tissue fluid homeostasis and the immunological response to inflammation. The effects of lymphatic drainage dysfunction on periodontitis have not been well studied. Here we show that lymphatic vessel endothelial receptor 1 (LYVE1)+ /podoplanin (PDPN)+ lymphatic vessels (LVs) are increased in the periodontal tissues, with accumulation close to the alveolar bone surface, in two murine periodontitis models: rheumatoid arthritis (RA)-associated periodontitis and ligature-induced periodontitis. Further, PDPN+ /alpha-smooth muscle actin (αSMA)- lymphatic capillaries are increased, whereas PDPN+ /αSMA+ collecting LVs are decreased significantly in the inflamed periodontal tissues. Both mouse models of periodontitis have delayed lymph flow in periodontal tissues, increased TRAP-positive osteoclasts, and significant alveolar bone loss. Importantly, the local administration of adeno-associated virus for vascular endothelial growth factor C, the major growth factor that promotes lymphangiogenesis, increases the area and number of PDPN+ /αSMA+ collecting LVs, promotes local lymphatic drainage, and reduces alveolar bone loss in both models of periodontitis. Lastly, LYVE1+ /αSMA- lymphatic capillaries are increased, whereas LYVE1+ /αSMA+ collecting LVs are decreased significantly in gingival tissues of patients with chronic periodontitis compared with those of clinically healthy controls. Thus, our findings reveal an important role of local lymphatic drainage in periodontal inflammation-mediated alveolar bone loss. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.


Asunto(s)
Pérdida de Hueso Alveolar/prevención & control , Proceso Alveolar/metabolismo , Periodontitis Crónica/terapia , Terapia Genética , Linfa/metabolismo , Vasos Linfáticos/metabolismo , Maxilar/metabolismo , Factor C de Crecimiento Endotelial Vascular/biosíntesis , Factor C de Crecimiento Endotelial Vascular/genética , Pérdida de Hueso Alveolar/genética , Pérdida de Hueso Alveolar/metabolismo , Pérdida de Hueso Alveolar/patología , Proceso Alveolar/patología , Animales , Estudios de Casos y Controles , Periodontitis Crónica/genética , Periodontitis Crónica/metabolismo , Periodontitis Crónica/patología , Modelos Animales de Enfermedad , Humanos , Vasos Linfáticos/patología , Masculino , Maxilar/patología , Ratones Endogámicos C57BL , Ratones Transgénicos , Osteoclastos/metabolismo , Osteoclastos/patología , Factor de Necrosis Tumoral alfa/genética
6.
Braz Oral Res ; 34: e016, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32130363

RESUMEN

Horizontal bone loss after tooth extraction is a common finding that demands bone reconstruction in various cases. The aim of this study was to assess the horizontal alveolar status in partially and completely edentulous patients using cone-beam computed tomography (CBCT). In total, 1516 CBCT scans of 1404 adult patients were analyzed. Assessment of the images was performed in accordance with the previously published horizontal alveolar change (HAC) classification, which categorizes horizontal bone defects into four classes: HAC 1, HAC 2, HAC 3 and HAC 4 (from the least severe to the most severe condition). Analysis of 1048 scans from partially edentulous patients presented a distribution of 63.55%, 22.14%, 13.36% and 0.95% in HAC 1, HAC 2, HAC 3 and HAC 4, respectively. Analysis of 468 scans from completely edentulous patient images presented a distribution of 19.87%, 28.63%, 41.67% and 9.83% in HAC 1, HAC 2, HAC 3 and HAC 4, respectively. Based on these results, as in HAC 4, no cancellous bone was found between the cortical buccal and lingual/palatal bone plates, it seems reasonable to state that the absence of cancellous bone is higher in completely edentulous patients than in partially edentulous patients. Therefore, the absence of cancellous bone seems to be higher in completely edentulous than in partially edentulous patients.


Asunto(s)
Pérdida de Hueso Alveolar/epidemiología , Pérdida de Hueso Alveolar/patología , Proceso Alveolar/patología , Boca Edéntula/epidemiología , Boca Edéntula/patología , Adolescente , Adulto , Anciano , Pérdida de Hueso Alveolar/diagnóstico por imagen , Proceso Alveolar/diagnóstico por imagen , Aumento de la Cresta Alveolar , Brasil/epidemiología , Hueso Esponjoso/diagnóstico por imagen , Hueso Esponjoso/patología , Tomografía Computarizada de Haz Cónico/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Boca Edéntula/diagnóstico por imagen , Prevalencia , Estudios Retrospectivos , Adulto Joven
7.
Braz Oral Res ; 34: e012, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32049112

RESUMEN

Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Asunto(s)
Lipopéptidos/farmacología , Periodontitis/etiología , Periodontitis/patología , Receptor Toll-Like 2/antagonistas & inhibidores , Pérdida de Hueso Alveolar/etiología , Pérdida de Hueso Alveolar/patología , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/patología , Animales , Modelos Animales de Enfermedad , Encía/efectos de los fármacos , Encía/patología , Gingivitis/etiología , Gingivitis/patología , Masculino , Ratones Endogámicos C57BL , Osteoclastos/efectos de los fármacos , Osteoclastos/fisiología , Periodontitis/microbiología , Distribución Aleatoria , Fosfatasa Ácida Tartratorresistente , Factores de Tiempo , Microtomografía por Rayos X
8.
Exp Neurol ; 326: 113176, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-31926167

RESUMEN

Epidemiological studies suggest that chronic periodontitis (CP) is closely associated with the incidence and progression of cognitive impairment. The present study investigated the causal relationship between CP and cognitive decline and the underlying mechanism in mice. Long-term ligature around the left second maxillary molar tooth was used to induce CP in mice. Severe alveolar bone loss and inflammatory changes were observed in gingival tissues, accompanied by progressive cognitive deficits during a 12-month period. We also observed cerebral neuronal and synaptic injury and glial activation in this mouse model of CP. Furthermore, CP mice exhibited significant dysbiosis of the oral and gut microbiota, disruption of the intestinal barrier and blood-brain barrier, increases in the serum contents of proinflammatory cytokines and lipopolysaccharide (LPS), and increases in brain LPS levels, Toll-like receptor 4 (TLR4) expression, nuclear factor-κB (NF-κB) nuclear translocation and proinflammatory cytokine mRNA levels. These results indicate that CP may directly induce progressive cognitive decline and its mechanism is probably related to microbiota-gut-brain axis disorders, LPS/TLR4/NF-κB signaling activation and neuroinflammatory responses in mice. Therefore, the microbiota-gut-brain axis may provide the potential strategy for the prevention and treatment of CP-associated cognitive impairment.


Asunto(s)
Encefalopatías/etiología , Periodontitis Crónica/complicaciones , Trastornos del Conocimiento/etiología , Microbioma Gastrointestinal , Proceso Alveolar/patología , Animales , Barrera Hematoencefálica , Encefalopatías/psicología , Enfermedad Crónica , Periodontitis Crónica/psicología , Trastornos del Conocimiento/psicología , Citocinas/sangre , Lipopolisacáridos/sangre , Masculino , Ratones , FN-kappa B , Neuroglía/patología , Neuronas/patología , Transducción de Señal , Sinapsis/patología , Receptor Toll-Like 4
9.
Inflammation ; 43(1): 220-230, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31720989

RESUMEN

Periodontitis is an inflammation characterized by alveolar bone resorption caused by imbalance in bone homeostasis. It is known that autophagy is related to inflammation and bone metabolism. However, whether autophagy inhibitors could be used for periodontitis in animal models remains unknown. We investigated the role of two classical autophagy inhibitors, 3-methyladenine (3-MA) and chloroquine (CQ), on the development of rat experimental periodontitis in terms of the bone loss (micro-CT), the number of inflammatory cells (hematoxylin and eosin staining), and the osteoclastic activity (tartrate-resistant acid phosphatase staining). Expression of autophagy-related genes and nuclear factor kappa B p65 (NF-κB p65) were assessed by immunohistochemistry. Expression of Beclin-1 and microtubule-associated proteins 1A/1B light chain 3 (LC3) were analyzed by Western blot. To further observe the effect of autophagy inhibitors on osteoclasts (OCs) in vitro, bone marrow-derived mononuclear macrophages were used. Together, these findings indicated that topical administration of 3-MA or CQ reduced the infiltration of inflammatory cells and alveolar bone resorption in experimental periodontitis. Furthermore, 3-MA and CQ may attenuate activation of OCs by autophagy. Therefore, 3MA and CQ may have prophylactic and therapeutic potential for inflammation and alveolar bone resorption in periodontitis in the future.


Asunto(s)
Adenina/análogos & derivados , Pérdida de Hueso Alveolar/prevención & control , Proceso Alveolar/efectos de los fármacos , Antiinflamatorios/farmacología , Autofagia/efectos de los fármacos , Cloroquina/farmacología , Osteoclastos/efectos de los fármacos , Periodontitis/prevención & control , Adenina/farmacología , Pérdida de Hueso Alveolar/metabolismo , Pérdida de Hueso Alveolar/microbiología , Pérdida de Hueso Alveolar/patología , Proceso Alveolar/metabolismo , Proceso Alveolar/microbiología , Proceso Alveolar/patología , Animales , Proteínas Relacionadas con la Autofagia/metabolismo , Células Cultivadas , Modelos Animales de Enfermedad , Masculino , Osteoclastos/metabolismo , Osteoclastos/microbiología , Osteoclastos/patología , Osteogénesis/efectos de los fármacos , Periodontitis/metabolismo , Periodontitis/microbiología , Periodontitis/patología , Porphyromonas gingivalis , Ratas Sprague-Dawley , Factor de Transcripción ReIA/metabolismo
10.
Biochem Biophys Res Commun ; 522(2): 471-478, 2020 02 05.
Artículo en Inglés | MEDLINE | ID: mdl-31780258

RESUMEN

The inhibition of high glucose on the proliferation and differentiation of osteoblast in alveolar bone are well documented. However, a comprehensive study focused on the molecular mechanisms is still unknown. Recent studies have revealed that caspase-1 participates in the pathological processes of hepatic injury, cancers and diabetes related complications. However, the relationship between pyroptosis and proliferation and differentiation of osteoblasts has not been investigated. This study aimed to explore the possible pyroptosis participating in the inhibition of high glucose on the proliferation and differentiation of osteoblast in alveolar bone. The diabetes model was constructed both in vitro and in vivo to detect the expression of pyroptosis related factors. These results show that high glucose inhibits proliferation and differentiation of osteoblast in alveolar bone through pyroptosis pathway. Furthermore, caspase-1 inhibitor was co-administered with high glucose in ME3T3-E1 cells, which shows that caspase-1 inhibitor could repress effect of high glucose on the proliferation and differentiation of osteoblast. In conclusion, High glucose could activate the pyroptosis through the caspase-1/GSDMD/IL-1ß pathway to inhibit the proliferation and differentiation of osteoblast in alveolar bone, which provides a theoretical basis for clinical treatment of alveolar bone disease in diabetic patients.


Asunto(s)
Proceso Alveolar/patología , Diferenciación Celular/efectos de los fármacos , Glucosa/toxicidad , Osteoblastos/patología , Piroptosis/efectos de los fármacos , Proceso Alveolar/efectos de los fármacos , Animales , Caspasa 1/metabolismo , Inhibidores de Caspasas/farmacología , Línea Celular , Proliferación Celular/efectos de los fármacos , Diabetes Mellitus Experimental/patología , Interleucina-1beta/metabolismo , Ratones , Osteoblastos/efectos de los fármacos , Osteoblastos/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/efectos de los fármacos , beta Catenina/metabolismo
11.
Biomed Res Int ; 2019: 3295756, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31886202

RESUMEN

Platelet-rich fibrin (PRF) is an autologous platelet concentrate that consists of cytokines, platelets, leukocytes, and circulating stem cells. It has been considered to be effective in bone regeneration and is mainly used for oral and maxillofacial bone. Although currently the use of PRF is thought to support alveolar ridge preservation, there is a lack of evidence regarding the application of PRF in osteogenesis. In this paper, we will provide examples of PRF application, and we will also summarize different measures to improve the properties of PRF for achieving better osteogenesis. The effect of PRF as a bone graft material on osteogenesis based on laboratory investigations, animal tests, and clinical evaluations is first reviewed here. In vitro, PRF was able to stimulate cell proliferation, differentiation, migration, mineralization, and osteogenesis-related gene expression. Preclinical and clinical trials suggested that PRF alone may have a limited effect. To enlighten researchers, modified PRF graft materials are further reviewed, including PRF combined with other bone graft materials, PRF combined with drugs, and a new-type PRF. Finally, we will summarize the common shortcomings in the application of PRF that probably lead to application failure. Future scientists should avoid or solve these problems to achieve better regeneration.


Asunto(s)
Proceso Alveolar , Regeneración Ósea/efectos de los fármacos , Trasplante Óseo , Procedimientos Quirúrgicos Orales , Osteogénesis/efectos de los fármacos , Fibrina Rica en Plaquetas , Proceso Alveolar/metabolismo , Proceso Alveolar/patología , Proceso Alveolar/cirugía , Animales , Trasplante Óseo/clasificación , Trasplante Óseo/métodos , Humanos , Procedimientos Quirúrgicos Orales/clasificación , Procedimientos Quirúrgicos Orales/métodos
12.
Rev. medica electron ; 41(6): 1424-1437, oct.-dic. 2019. tab
Artículo en Español | LILACS, CUMED | ID: biblio-1094140

RESUMEN

RESUMEN Introducción: los injertos óseos constituyen una de las técnicas más utilizadas en la cirugía reconstructiva implantológicas, son muy utilizadas para el reemplazo del hueso perdido por traumatismos, procesos patológicos congénitos o adquiridos y atrofia, son los injertos óseos autógenos o autólogos. Objetivo: caracterizar los pacientes con rebordes atróficos que necesitaron ser rehabilitados en implantología oral como alternativa de tratamiento en la consulta de Cirugía Máxilo Facial del Hospital Universitario "Faustino Pérez" y la Clínica "III Congreso del PCC", municipio Matanzas de septiembre del 2014 a julio de 2016. Material y Método: estudio prospectivo longitudinal. El universo fue de 20 pacientes mayores de 18 años de ambos sexos, que presentaron el diagnóstico de edentulismo parcial y atrofia alveolar. Se determinó por el interrogatorio, el examen clínico y los medios diagnósticos los síntomas y signos que caracterizaron esta entidad. Resultados: los traumatismos alveolares fue la causa que predominó en la pérdida dentaria, en el sexo masculino y en las edades de 18 a 37 años. La zona de mayor afectación fue la región anterior del maxilar superior y predominó la perdida de hueso en altura y en anchura y un gran número de injertos conservaron la cresta alveolar. Conclusiones: el uso de biomateriales en el tratamiento de pacientes con atrofia alveolar junto al injerto óseo fue satisfactorio en pacientes que necesitaron una base de sostén sobre la cual se colocaron los implantes dentales osteointegrados (AU).


SUMMARY Introduction: autogenous and autologous bone grafts are the elective material for replacing bones lost by trauma, congenital or acquired pathologic processes and atrophy. Objective: to characterize patients with atrophic rims needing rehabilitation in oral grafting as an alternative treatment in the Maxilla-Facial Surgery consultation of the University Hospital "Faustino Perez" and the Clinic "III Congreso del PCC", municipality of Matanzas, from September 2014 to July 2016. Materials and Methods: longitudinal prospective study. The universe was 20 patients aged 18 years and older, males and females, who presented the diagnosis of partial lack of teeth and alveolar atrophy. The symptoms and signs characterizing this entity were stated by questioning, physical examination and diagnostic means. Results: alveolar traumas were the predominant cause of dental lost in male patients aged 18-37 years. The most affected zone was the anterior region of the upper maxilla; bone lost in height and width predominated, and a great number of grafts conserved the alveolar crest. Conclusions: the use of biomaterials in the treatment of patients with alveolar atrophy together with bone graft was satisfactory in patients who needed a base support on which to put dental grafts (AU).


Asunto(s)
Humanos , Niño , Adolescente , Proceso Alveolar/patología , Aumento de la Cresta Alveolar , Alveolectomía , Injerto de Hueso Alveolar , Rehabilitación , Atrofia/diagnóstico , Atrofia/etiología , Atrofia/epidemiología , Cirugía Bucal , Epidemiología Descriptiva , Estudios Transversales , Estudio Observacional
13.
J Appl Oral Sci ; 27: e20180574, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31596365

RESUMEN

OBJECTIVES: Hypertension is one of the main causes of premature death in the world; also, it is associated with several bone alterations. Preclinical studies have demonstrated delayed alveolar bone healing in hypertensive rats. However, losartan has been favorable for consolidation of bone grafts and reduction in active periodontitis. Therefore, losartan is suggested to be effective in bone formation stages, as well as in the synthesis of matrix proteins and mineralization. To evaluate the alveolar bone dynamics in hypertensive rats treated with losartan by laser confocal microscopy and histological analysis. METHODOLOGY: Thirty-two rats, 16 spontaneously hypertensive rats (SHR) and 16 Wistar albinus rats, treated or not with losartan (30 mg/kg/day) were used. Calcein fluorochrome at 21 days and alizarin red fluorochrome at 49 days were injected in rats (both 20 mg/kg). The animals were submitted to euthanasia 67 days after treatment, and then the right maxilla was removed for laser confocal microscopy analysis and the left maxilla for histological analysis. RESULTS: This study showed a greater calcium marking in normotensive animals treated with losartan in relation to the other groups. Laser confocal microscopy parameters showed higher values of bone volume formed, mineralized surface, active surface of mineralization and bone formation rate in normotensive animals treated with losartan. However, a smaller mineralized surface was observed in all hypertensive animals. CONCLUSION: Losartan can improve bone mineralization parameters under normal physiological conditions, but the same anabolic effect does not occur under hypertension.


Asunto(s)
Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/fisiopatología , Antihipertensivos/farmacología , Hipertensión/fisiopatología , Losartán/farmacología , Proceso Alveolar/patología , Animales , Presión Sanguínea/efectos de los fármacos , Regeneración Ósea/efectos de los fármacos , Calcificación Fisiológica/efectos de los fármacos , Fluoresceínas/análisis , Masculino , Microscopía Confocal , Osteogénesis/efectos de los fármacos , Ratas Endogámicas SHR , Ratas Wistar , Reproducibilidad de los Resultados , Factores de Tiempo
14.
Calcif Tissue Int ; 105(6): 619-629, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31535164

RESUMEN

Smoking is a major risk factor for dental implant failure. In addition to higher marginal bone loss around implants, the cellular and molecular responses to injury and implant physicochemical properties are also differentially affected in smokers. The purpose of this work is to determine if smoking impairs bone microstructure and extracellular matrix composition within the dental alveolar socket after tooth extraction. Alveolar bone biopsies obtained from Smokers (> 10 cigarettes per day for at least 10 years) and Ctrl (never-smokers), 7-146 months after tooth extraction, were investigated using X-ray micro-computed tomography, backscattered electron scanning electron microscopy, and Raman spectroscopy. Both Smokers and Ctrl exhibited high inter- and intra-individual heterogeneity in bone microstructure, which varied between dense cortical and porous trabecular architecture. Regions of disorganised/woven bone were more prevalent during early healing. Remodelled lamellar bone was predominant at longer healing periods. Bone mineral density, bone surface-to-volume ratio, mineral crystallinity, the carbonate-to-phosphate ratio, the mineral-to-matrix ratio, the collagen crosslink ratio, and the amounts of amino acids phenylalanine and proline/hydroxyproline were also comparable between Smokers and Ctrl. Bone microstructure and composition within the healing dental alveolar socket are not significantly affected by moderate-to-heavy smoking.


Asunto(s)
Pérdida de Hueso Alveolar/patología , Proceso Alveolar/patología , Regeneración Ósea/fisiología , Fumar/efectos adversos , Alveolo Dental/patología , Adulto , Anciano , Sustitutos de Huesos/química , Colágeno/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Minerales/metabolismo , Extracción Dental/métodos , Cicatrización de Heridas/fisiología
15.
Shanghai Kou Qiang Yi Xue ; 28(3): 307-311, 2019 Jun.
Artículo en Chino | MEDLINE | ID: mdl-31489421

RESUMEN

PURPOSE: To study the height of alveolar ridge and the distance between the wall of maxillary sinus on the curve of the dental arch, and to explore the guiding significance for maxillary sinus surgery and edentulous jaw implant surgery. METHODS: Cone-beam CT(CBCT) data of 105 objects were selected to measure the height of alveolar ridge and the maximum bone internal distance between the wall of maxillary sinus 10 mm and 12 mm above the alveolar crest horizontally on the curve of the dental arch. Forty-nine objects with alveolar ridge height on the molar area less than or equal to 10 mm were included for further investigation. The distance between the maxillary sinus wall, and the angle between the maxillary sinus wall on the horizontal plane were measured and calculated. SPSS 22.0 software package was used for statistical analysis of the data. RESULTS: The mean value of the alveolar ridge height in the posterior teeth area was first premolar > second premolars > second molars > first molars. The mean value of the horizontal distance between the wall of sinus 10 mm and 12 mm above the alveolar crest horizontally was (82.40±27.56) mm and (70.54±29.70) mm in 105 objects, while (67.85±28.53) mm and (52.75±24.90) mm in 49 included objects, respectively. The mean value of the angle between the maxillary sinus wall on the horizontal plane was (23.55±39.13)°. There was no significant difference between males and females. However, the horizontal distance had statistically significant differences between different groups and in different horizontal levels of the same group. CONCLUSIONS: CBCT is helpful to establish implant surgical plan, and carry out maxillary sinus lift and edentulous jaw implant surgery.


Asunto(s)
Proceso Alveolar , Tomografía Computarizada de Haz Cónico , Boca Edéntula , Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/patología , Femenino , Humanos , Masculino , Maxilar , Seno Maxilar , Boca Edéntula/diagnóstico por imagen
16.
J Oral Maxillofac Surg ; 77(12): 2494-2502, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31520586

RESUMEN

PURPOSE: The purpose of the present study was to assess the relationship between the posterior superior alveolar artery (PSAA), which plays an important role in the maxillary sinus blood supply, and maxillary sinus pathologic entities using cone-beam computed tomography (CBCT). MATERIALS AND METHODS: The CBCT data from 325 patients (650 sinuses), aged 18 to 55 years, who had been referred to the Department of Dentomaxillofacial Radiology from 2015 to 2017 were evaluated retrospectively. For the PSAA examination, the axial, coronal, and sagittal CBCT images were evaluated for detection of an osseous canal in the lateral wall of the maxillary sinus and the prevalence, position, and location of the canal. In addition, the presence of maxillary sinus pathologic features was analyzed, and the relationship with the PSAA was investigated. The relationship among the categorical variables was tested using the χ2 test. RESULTS: The study group consisted of 325 patients, including 155 men (47.7%) and 170 women (52.3%). The PSAA was observed in 80.6% of the patients. Of the detected PSAAs, 79.0% were bilateral and 21.0% were unilateral and 56.3% were intraosseous and 40.7% were submembranous. A significant positive correlation was detected between the presence of a PSAA and patient age (P < .05). The prevalence of sinus pathologic entities was 73.2% of all patients. The most common sinus pathologic finding was localized mucosal thickening (53.5%). A significant relationship was found between the presence of PSAA and maxillary sinus pathologic entities (P < .001). CONCLUSIONS: The presence of a PSAA might be a predisposing factor that increases the susceptibility to maxillary sinus pathologic entities. CBCT is an effective imaging method in the evaluation of the maxillary sinuses.


Asunto(s)
Arterias , Tomografía Computarizada de Haz Cónico , Seno Maxilar , Adolescente , Adulto , Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/patología , Arterias/anatomía & histología , Arterias/diagnóstico por imagen , Femenino , Humanos , Masculino , Seno Maxilar/irrigación sanguínea , Seno Maxilar/diagnóstico por imagen , Seno Maxilar/patología , Persona de Mediana Edad , Estudios Retrospectivos , Adulto Joven
17.
Forensic Sci Int ; 301: e38-e43, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31174898

RESUMEN

The removal of a tooth is a common clinical procedure documented in an individual's dental record. A photographic and radiographic series of healing alveolar ridges/sockets are presented. The history of a documented extraction and a concordant finding of healing alveolar bone could assist in strengthening an association between a set of unidentified remains and an individual.


Asunto(s)
Proceso Alveolar/patología , Extracción Dental , Alveolo Dental/patología , Cicatrización de Heridas , Odontología Forense/métodos , Humanos , Cambios Post Mortem , Alveolo Dental/diagnóstico por imagen
18.
J Plast Reconstr Aesthet Surg ; 72(8): 1411-1417, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31078415

RESUMEN

BACKGROUND: It is debatable whether rhinoplasty is necessary during a primary operation for cleft lip. However, many surgeons believe that rhinoplasty should be performed simultaneously for severe deformities. We investigated whether alveolar cleft severity is involved in nasal deformity. METHODS: Forty-three patients were assessed for alveolar cleft severity using maxillary plaster models prepared during primary cheiloplasty. We conducted morphological assessments of nasal deformities using three-dimensional photogrammetry. Patients were divided into two groups according to alveolar cleft severity: group A, overlap of the alveolar segments; group B, nonoverlap of the alveolar segments. Nasal asymmetry was assessed by measuring distances between landmarks around the nostrils and the columellar angle. These measurements were compared between the groups. The correlations between the columellar angle and the ratios of the five cleft side/non-cleft side distances and the correlation of each ratio were analyzed. RESULTS: Groups A and B included 21 and 22 patients, respectively. Group A demonstrated superior deviation of the alar base on the non-cleft side than that of the alar base on the cleft side (p < 0.05). No other statistically significant differences were observed. Group A had more severe nasal deformity. Columellar angle and nostril base width demonstrated correlation. CONCLUSION: In an uncorrected, unoperated unilateral cleft lip nasal deformity, alar base deformity is affected by deformity of the alveolar segments.


Asunto(s)
Proceso Alveolar/patología , Labio Leporino/patología , Maxilar/patología , Nariz/anomalías , Nariz/patología , Proceso Alveolar/cirugía , Antropometría , Labio Leporino/cirugía , Femenino , Humanos , Imagenología Tridimensional , Lactante , Masculino , Maxilar/cirugía , Modelos Anatómicos , Nariz/cirugía , Fotogrametría , Estudios Retrospectivos , Rinoplastia
19.
J Oral Implantol ; 45(4): 334-340, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31042455

RESUMEN

We aimed to conduct an analysis of the systematic reviews (SRs) in literature about the implant survival rate (ISR) and marginal bone loss (MBL) in diabetic and nondiabetic patients. This work was registered in The International Prospective Register of Systematic Reviews (CRD42018095314) and was developed following Preferred Reporting Items for Systematic Reviews and Meta-Analyses and the Cochrane Library Handbook. A search was performed on PubMed, Cochrane, Scopus, Embase, and LILACS. The PICO (problem/patient/population, intervention/indicator, comparison, outcome) question was "Do the survival rates of dental implants and marginal bone loss differ between diabetic and nondiabetic patients?" A total of 130 articles were retrieved. After eliminating repetitions, 118 were reviewed. Finally, 6 SRs were included. All the reviews indicated that there is no effect of diabetes on the ISR; however, a negative effect of the disease can be observed in MBL. Analysis of the quality of the studies was performed using the assessment of SRs in dentistry (Glenny Scale) and Assessing the Methodological Quality of Systematic Reviews (AMSTAR 2). Glenny Scale showed a moderate to high quality of the included studies. In contrast, AMSTAR 2 pointed out a critically low level for 4 studies, with no study fulfilling the criteria for high quality. It may be concluded that there is no effect of diabetes on the ISR; however, a negative effect of the disease can be observed on MBL.


Asunto(s)
Implantes Dentales , Diabetes Mellitus , Revisiones Sistemáticas como Asunto , Proceso Alveolar/patología , Humanos , Estudios Prospectivos , Tasa de Supervivencia
20.
Int Orthod ; 17(2): 287-295, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-31015017

RESUMEN

OBJECTIVES: The purpose of this study was to investigate differences in the thickness of the labial bone overlying the maxillary and mandibular incisors in class I, II, and III skeletal classifications using three-dimensional cone-beam computed tomographs (CBCT) technology. METHODS: Pretreatment CBCTs of 54 Caucasian subjects (44 males, 10 females) were collected from the archives of a graduate orthodontic clinic. The subjects were divided into three groups based on their skeletal classification. CBCT scans were oriented to the long axis of each maxillary and mandibular incisor from the root apex to incisal tip and the axial inclination of each incisor was measured and recorded. Labial bone thickness was measured at the apex (A) and at the midpoint (MP) on each maxillary and mandibular incisor. A linear measurement, perpendicular to the long axis, was recorded from the labial bone surface to the most anterior root surface at two points. The effects of skeletal classification, jaw, incisor position, and side on A, MP, and Inclination were evaluated using mixed-model ANOVA. A 5% significance level was used for all tests. RESULTS: Significantly greater bone thickness at the apex and midpoint was reported in class III individuals than class I or II. Central incisors had significantly greater bone thickness at both the root apex and midpoint. Significantly greater bone thickness was noted in the mandible at the level of the apex. At the level of the midpoint, significantly greater thickness was reported in the maxilla. There was a positive correlation between thickness and angle within each jaw-incisor combination for class I patients and for central incisors in class III patients. CONCLUSION: The thickness of labial alveolar bone over the incisors varies based on the underlying skeletal discrepancy in each patient. Skeletal discrepancy influences the inclination of the maxillary and mandibular incisors.


Asunto(s)
Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/patología , Imagenología Tridimensional/métodos , Incisivo/anatomía & histología , Incisivo/diagnóstico por imagen , Maloclusión/diagnóstico por imagen , Maloclusión/patología , Adulto , Cefalometría , Tomografía Computarizada de Haz Cónico/métodos , Femenino , Humanos , Masculino , Maloclusión/terapia , Mandíbula/anatomía & histología , Mandíbula/diagnóstico por imagen , Maxilar/anatomía & histología , Maxilar/diagnóstico por imagen , Ápice del Diente/anatomía & histología , Ápice del Diente/diagnóstico por imagen , Adulto Joven
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