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1.
Food Chem ; 308: 125601, 2020 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-31670190

RESUMEN

The aim of this work was to analyse the hypotensive effect of amaranth protein/peptides on spontaneously hypertensive rats (SHR). The mechanism of action of these peptides was studied in vivo and ex vivo. We also tested the effect of protection against gastrointestinal digestion (GID) exerted by an O:W emulsion on the integrity of the antihypertensive peptides. All samples tested produced a decrease in blood pressure (SBP). The animals treated with emulsion (GE) and emulsion + peptide (GE+VIKP) showed the most significant reduction in the SBP (42 ±â€¯2 mmHg and 35 ±â€¯2 mmHg, respectively). The results presented suggest that after GID, a variety of peptides with biological activities were released or were resistant to this process. These peptides play a role in the regulation of the SBP by acting on plasma ACE, plasma renin and the vascular system. These results support the use of amaranth protein/peptides in the elaboration of functional foods for hypertensive individuals.


Asunto(s)
Amaranthus/química , Antihipertensivos/farmacología , Hipertensión/tratamiento farmacológico , Péptidos/farmacología , Sistema Renina-Angiotensina/efectos de los fármacos , Animales , Antihipertensivos/uso terapéutico , Presión Sanguínea/efectos de los fármacos , Masculino , Péptidos/uso terapéutico , Proteínas de Plantas/farmacología , Proteínas de Plantas/uso terapéutico , Ratas , Ratas Endogámicas SHR
2.
Mem Inst Oswaldo Cruz ; 114: e190326, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31859703

RESUMEN

BACKGROUND: Severe bacterial infections initiate inadequate inflammation that leads to disseminated intravascular coagulation and death. OBJECTIVES: To evaluate the influence of bacterial infection on blood viscosity and red blood cells (RBCs) morphology, and the ability of Calotropis procera proteins (CpLP) to prevent the patho-hemorheology in infected animals. METHODS: Rheology of blood, atomic force microscopy measurements on specific blood elements and blood count were performed to examine changes in blood viscosity, RBCs morphology, platelets activation, and RBCs indices. FINDINGS: Infected mice hold their blood rheological behaviour as compared to that of the control group. However, they presented hyperactivated platelets, RBCs at different stages of eryptosis, and variation on RBCs indices. CpLP administration in healthy animals altered blood behaviour from pseudoplastic to Bingham-like fluid. Such effect disappeared over time and by inhibiting its proteases. No alterations were observed in RBCs morphology or platelets. Treatment of infected animals with CpLP prevented the changes in RBCs indices and morphology. MAIN CONCLUSIONS: The inflammatory process triggered by bacterial infection induced pathological changes in RBCs and platelets activation. Treatment of infected animals with CpLP prevented the emergence of RBCs abnormal morphology and this may have implications in the protective effect of CpLP, avoiding animal death.


Asunto(s)
Viscosidad Sanguínea/efectos de los fármacos , Calotropis/química , Eritrocitos/microbiología , Hemorreología/efectos de los fármacos , Proteínas de Plantas/farmacología , Salmonella typhi , Fiebre Tifoidea/sangre , Animales , Modelos Animales de Enfermedad , Recuento de Eritrocitos , Eritrocitos/efectos de los fármacos , Masculino , Ratones , Microscopía de Fuerza Atómica , Proteínas de Plantas/aislamiento & purificación , Índice de Severidad de la Enfermedad
3.
J Agric Food Chem ; 67(37): 10423-10431, 2019 Sep 18.
Artículo en Inglés | MEDLINE | ID: mdl-31487168

RESUMEN

Plants often produce antifungal peptides and proteins in response to infection. Also wheat, which is the main ingredient of bread dough, contains such components. Here, we show that while some industrial strains of the baker's yeast Saccharomyces cerevisiae can efficiently ferment dough, some other strains show much lower fermentation capacities because they are sensitive to a specific wheat protein. We purified and identified what turned out to be a thaumatin-like protein through a combination of activity-guided fractionation, cation exchange chromatography, reversed-phase HPLC, and LC-MS/MS. Recombinant expression of the corresponding gene and testing the activity confirmed the inhibitory activity of the protein.


Asunto(s)
Proteínas de Plantas/química , Proteínas de Plantas/farmacología , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/crecimiento & desarrollo , Triticum/química , Cromatografía Liquida , Fermentación , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/metabolismo , Saccharomyces cerevisiae/metabolismo , Espectrometría de Masas en Tándem , Triticum/genética , Triticum/metabolismo , Triticum/microbiología
4.
Mar Drugs ; 17(8)2019 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-31374944

RESUMEN

Microalgae are considered as excellent candidates for bioactive compounds, yet microalgal residues remaining after the extraction of one or two compounds are usually discarded, which is not economical. This study demonstrates the alkaline extraction of proteins from Chlorella pyrenoidosa residue after lipid and pigment extractions, and their functional properties. Single-factor experiments and response surface methodology were used to obtain the optimal conditions for protein extraction. Based on our results, a maximum protein yield of 722.70 mg/g, was obtained under the following extraction conditions: sodium hydroxide concentration 7.90%, extraction temperature 70.00 °C, extraction time 34.80 min, and microalgal residue concentration 8.20 mg/mL. The molecular weight of microalgal residue protein isolate (MRPI) was mainly distributed at the regions of 0.18-0.50 kDa, 0.50-1.50 kDa, and 1.50-5.00 kDa. The essential amino acid content was greater than the values recommended by FAO/WHO standards; a high essential amino acid index value (1.49) was another good indication that MRPI is suitable for human consumption. Moreover, MRPI exhibited excellent emulsifying properties and antioxidant activity, which suggests it may be useful as an emulsifying agent and antioxidant. These findings could improve the extraction methods of functional protein from microalgal residue and add value to microalgae-based bioactive compound production processes.


Asunto(s)
Chlorella/química , Microalgas/química , Extractos Vegetales/farmacología , Proteínas de Plantas/farmacología , Secuencia de Aminoácidos , Aminoácidos Esenciales/química , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Emulsionantes/química , Emulsionantes/aislamiento & purificación , Emulsionantes/farmacología , Alimentos Funcionales , Lípidos/aislamiento & purificación , Peso Molecular , Estrés Oxidativo/efectos de los fármacos , Pigmentos Biológicos/aislamiento & purificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Hidróxido de Sodio/química , Temperatura Ambiental
5.
Artif Cells Nanomed Biotechnol ; 47(1): 3259-3264, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31368822

RESUMEN

Impairment of type II collagen caused by MMPs in response to overproduction of IL-1ß is an important step in the pathological progression of osteoarthritis (OA). Lunasin, a well-known peptide present in the soybean, has displayed a positive impact on numerous physiological functions. Little information in the effects of lunasin on cartilage degradation has been sought in clinical research before. Here, we report that lunasin suppressed the increase in MMP-3 and MMP-13 caused by IL-1ß. In addition, we found that lunasin could prevent the decrease in TIMP-1 and TIMP-2 expressions caused by IL-1ß. Notably, lunasin suppressed reduction of type II collagen, the basis for articular cartilage. Lunasin also attenuated activation of the JAK2/STAT1/IRF-1 pathway. These effects of lunasin suggest that it might become a promising therapeutic agent for chondro-protective therapy.


Asunto(s)
Colágeno Tipo II/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Metaloproteinasa 13 de la Matriz/genética , Metaloproteinasa 13 de la Matriz/metabolismo , Metaloproteinasa 3 de la Matriz/genética , Metaloproteinasa 3 de la Matriz/metabolismo , Proteínas de Plantas/farmacología , Cartílago Articular/citología , Proliferación Celular/efectos de los fármacos , Condrocitos/citología , Condrocitos/efectos de los fármacos , Condrocitos/metabolismo , Relación Dosis-Respuesta a Droga , Humanos , Interleucina-1beta/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo
6.
J Agric Food Chem ; 67(33): 9354-9361, 2019 Aug 21.
Artículo en Inglés | MEDLINE | ID: mdl-31339706

RESUMEN

As a popular ingredient for western and traditional Chinese medicine, the root and rhizome of Chinese licorice (Glycyrrhiza uralensis Fisch.) is often administered in the form of a decoction. The protein nanoparticles (NPs) self-assembled during the process of decoction. A major constitutive protein (GLP) was purified and determined to have a molecular weight of 28 kDa with an N-terminal sequence of NPDGL IACYC GQYCW. Over 80% of the purified GLP self-assembled into spherical NPs with diameters of 74.1 ± 0.7 nm and ζ-potential of -24.3 ± 1.7 mV when boiled in Tris-HCl buffer (pH = 7.9, 20 mM) at 100 °C for 60 min. Each nanoparticle was estimated by the SEC-MALLS approach to be composed of approximately 23 protein molecules. The NPs and GLP showed low cellular toxicity upon four types of cells including MDCK, L-02, HepG2, and Caco2 cells, while the NPs promoted proliferation of normal hepatocytes by 67%. The NPs solubilized the insoluble astragaloside IV by encapsulation. The results suggest a great potential for GLP-NPs as a promising prototype of a type of drug vehicle, a novel source of bioactive nanomaterials from herbal proteins, as well as a new mode of function with herbal components.


Asunto(s)
Glycyrrhiza/química , Nanopartículas/química , Extractos Vegetales/química , Proteínas de Plantas/química , Células CACO-2 , Proliferación Celular/efectos de los fármacos , Células Hep G2 , Calor , Humanos , Peso Molecular , Nanoestructuras/química , Extractos Vegetales/farmacología , Proteínas de Plantas/farmacología , Raíces de Plantas/química
7.
World J Gastroenterol ; 25(25): 3196-3206, 2019 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-31333311

RESUMEN

BACKGROUND: Colorectal cancer (CRC) is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China. Sporamin, a Kunitz-type trypsin inhibitor isolated from sweet potato, is a potential anti-cancer agent with activities against a number of malignant tumor cells in vitro. The liver secretes a myriad of endocrine factors that may facilitate the growth and transformation of tumors in the development of CRC. AIM: To investigate the effects of sporamin on liver morphology and biomarkers of xenografted CRC in the liver of athymic BALB/c mice. METHODS: Twenty-seven male BALB/c nude mice were randomly divided into control, vehicle, and sporamin groups. Mice in the latter two groups were intraperitoneally xenografted with LoVo colorectal carcinoma cells and intragastrically infused with saline or sporamin (0.5 g/kg body weight/d), respectively, for 3 wk. Hematoxylin and eosin (HE) staining of the sections was performed to observe morphological changes in hepatic tissue and real-time fluorescent quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA) were used to measure the expression of ß-catenin and vascular endothelial growth factor (VEGF) in the liver. RESULTS: Sporamin significantly reduced the number and weight of tumor nodules formed in the abdominal cavity. Compared with the vehicle group, the mean tumor weight (± SD) in the sporamin group was significantly reduced (0.44 ± 0.10 g vs 0.26 ± 0.15 g) and the total number of tumors decreased from 93 to 55. HE staining showed that enlargement of the nucleus and synthesis of proteins within hepatocytes, as well as infiltration of inflammatory cells into the liver, were attenuated by sporamin. Immunohistochemical staining and ELISA showed that the concentrations of ß-catenin and VEGF in the liver were significantly reduced by sporamin. Compared with the vehicle group, the expression of ß-catenin measured in integrated optical density units per area was reduced in the sporamin group (47.29 ± 9.10 vs 26.14 ± 1.72; P = 0.003). Expression of VEGF was also reduced after sporamin intervention from 20.78 ± 2.06 in the vehicle group to 15.80 ± 1.09 in the sporamin group (P = 0.021). Compared with the vehicle group, the concentration of ß-catenin decreased from 134.42 ± 22.04 pg/mL to 109.07 ± 9.65 pg/mL after sporamin intervention (P = 0.00002). qPCR indicated that compared to the vehicle group, relative mRNA expression of ß-catenin and VEGF in the liver of mice in the sporamin-treated group was significantly reduced to 71% ± 1% (P = 0.000001) and 23% ± 7% (P = 0.00002), respectively, of the vehicle group levels. CONCLUSION: Sporamin down-regulates the expression and secretion of ß-catenin and VEGF in the liver, which subsequently inhibits the transcription of downstream genes involved in cancer progression and angiogenesis.


Asunto(s)
Neoplasias Colorrectales/tratamiento farmacológico , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Neovascularización Patológica/tratamiento farmacológico , Péptidos/farmacología , Proteínas de Plantas/farmacología , Animales , Biomarcadores de Tumor/metabolismo , Línea Celular Tumoral , Neoplasias Colorrectales/irrigación sanguínea , Neoplasias Colorrectales/patología , Progresión de la Enfermedad , Regulación hacia Abajo/efectos de los fármacos , Humanos , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Neovascularización Patológica/patología , Péptidos/uso terapéutico , Proteínas de Plantas/uso terapéutico , Resultado del Tratamiento , Factor A de Crecimiento Endotelial Vascular/metabolismo , Ensayos Antitumor por Modelo de Xenoinjerto , beta Catenina/metabolismo
8.
Food Chem ; 299: 125165, 2019 Nov 30.
Artículo en Inglés | MEDLINE | ID: mdl-31306953

RESUMEN

In the present study, the antioxidant hydrolysates obtained from watermelon seed protein (WSP) after divergent ultrasound and ultrafiltration treatment were studied. The results showed that the slit divergent ultrasound (SDU, 20/28 KHz) pretreatment had considerable influence on the structure and enzymatic efficiency of WSP. Besides, compared with hydrolysates without ultrasonic and ultrafiltration treatment, watermelon protein hydrolysates with molecular weight <1 kDa (WSPHs-I) showed the highest antioxidant activities and could protect RAW 264.7 cells from H2O2-induced oxidative stress damage via activating the Nrf2/HO-1 pathway. Interestingly, WSPHs-I had good stability against oxidation at temperature under 100 °C or in the acidic or neutral condition and still exhibited strong antioxidant activity after simulated gastrointestinal digestion. Taken together, SDU pretreatment could significantly increase the antioxidant activities and stability of WSPHs by improving the structure and facilitating enzymolysis of the WSP.


Asunto(s)
Antioxidantes/farmacología , Citrullus/química , Proteínas de Plantas/química , Hidrolisados de Proteína/farmacología , Ultrasonido/métodos , Animales , Antioxidantes/química , Digestión/efectos de los fármacos , Peróxido de Hidrógeno/toxicidad , Ratones , Peso Molecular , Oxidación-Reducción , Proteínas de Plantas/farmacología , Hidrolisados de Proteína/química , Estabilidad Proteica , Células RAW 264.7 , Proteínas de Almacenamiento de Semillas/química , Semillas/química , Ultrafiltración
9.
Molecules ; 24(13)2019 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-31284657

RESUMEN

Two new 11,20-epoxybriaranes, fragilides P (1) and Q (2), as well as two known analogues, robustolide F (3) and juncin Z (4), were obtained from the gorgonian coral Junceella fragilis. The structures, including the absolute configurations of briaranes 1 and 2, were elucidated by using spectroscopic methods and comparing the spectroscopic and rotation data with those of known related analogues. Briarane 4 decreased the generation of superoxide anions by human neutrophils. The propionate group in 1 is rarely found.


Asunto(s)
Antozoos/química , Diterpenos/química , Proteínas de Plantas/química , Animales , Diterpenos/aislamiento & purificación , Diterpenos/farmacología , Modelos Moleculares , Conformación Molecular , Estructura Molecular , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Análisis Espectral
10.
Molecules ; 24(13)2019 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-31262066

RESUMEN

Grafting a bioactive peptide onto a disulfide-rich scaffold is a promising approach to improve its structure and metabolic stability. The ginkgo plant-derived ß-ginkgotide ß-gB1 is a highly unusual molecule: Small, hyperdisulfide, and found only in selected ancient plants. It also contains a conserved 16-amino-acid core with three interlocking disulfides, as well as a six-amino-acid inter-cysteine loop 2 suitable for grafting peptide epitopes. However, very little is known about this recently-discovered family of molecules. Here, we report the biophysical and functional characterizations of the ß-ginkgotide ß-gB1 from G. biloba. A circular dichroism spectroscopy analysis at 90 °C and proteolytic treatments of ß-gB1 supported that it is hyperstable. Data mining revealed that the ß-gB1 loop 2 contains the canonical LC3 interacting region (LIR) motif crucial for selective autophagy. Cell-based assays and pull-down experiments showed that ß-gB1 is an adaptogen, able to maintain cellular homeostasis through induced autophagosomes formation and to protect cells by targeting intracellular proteins from stress-mediated damage against hypoxia and the hypoxia-reoxygenation of induced cell death. This is the first report of an LIR-containing peptide natural product. Together, our results suggest that the plant-derived ß-ginkgotide is cytoprotective, capable of targeting intracellular proteins, and holds promise as a hyperdisulfide scaffold for engineering peptidyl therapeutics with enhanced structural and metabolic stability.


Asunto(s)
Citoprotección/efectos de los fármacos , Ginkgo biloba/química , Péptidos , Proteínas de Plantas , Animales , Autofagosomas/metabolismo , Hipoxia de la Célula/efectos de los fármacos , Línea Celular , Ratones , Estructura Molecular , Péptidos/química , Péptidos/farmacología , Proteínas de Plantas/química , Proteínas de Plantas/farmacología , Ratas
11.
Life Sci ; 231: 116535, 2019 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-31175857

RESUMEN

Latex proteins from P. pudica (LPPp) have anti-inflammatory activity. In the present study, LPPp was evaluated to protect animals against inflammatory ulcerative colitis (UC). UC was induced by intracolonic instillation of a 6% acetic acid solution and the animals received LPPp (10, 20 or 40 mg/kg) by intraperitoneal route 1 h before and 17 h after acetic acid injection. Eighteen hours after instillation of acetic acid, the mice were euthanized and the colons were excised to determine the wet weight, macroscopic and microscopic lesion scores, myeloperoxidase (MPO) activity, IL1-ß levels, glutathione (GSH) and malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity. The results revealed that LPPp treatment (40 mg/kg) had a protective effect on acetic acid-induced colitis by reducing the wet weight, macroscopic and microscopic scores of intestinal lesions and colonic MPO activity. Additionally, LPPp inhibited tissue oxidative stress, since decreases in GSH consumption, MDA concentration and SOD activity were observed. The treatment with LPPp reduced the levels of cytokine IL-1ß, contributing to the reduction of colon inflammation. Biochemical investigation showed that LPPp comprises a mixture of proteins containing proteinases, chitinases and proteinase inhibitors. These data suggest that LPPp has a protective effect against intestinal damage through mechanisms that involve the inhibition of inflammatory cell infiltration, cytokine release and oxidative stress.


Asunto(s)
Apocynaceae/química , Colitis/tratamiento farmacológico , Látex/farmacología , Proteínas de Plantas/farmacología , Ácido Acético , Animales , Apocynaceae/metabolismo , Colitis/inducido químicamente , Colitis/metabolismo , Colon/efectos de los fármacos , Citocinas/metabolismo , Glutatión/metabolismo , Inflamación/tratamiento farmacológico , Interleucina-1beta/metabolismo , Intestinos/patología , Látex/aislamiento & purificación , Masculino , Ratones , Estrés Oxidativo/efectos de los fármacos , Proteínas de Plantas/aislamiento & purificación , Sustancias Protectoras/farmacología , Factor de Necrosis Tumoral alfa/metabolismo
12.
J Med Food ; 22(10): 976-981, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31211650

RESUMEN

Fermentation has shown to be an effective technique in bioactive peptides release. That is why in this study antihypertensive, antithrombotic, and antioxidant activity was evaluated during amaranth proteins fermentation with Lactobacillus casei Shirota and Streptococcus thermophilus 54102 in mono and combined culture. During fermentation an increase of free amine groups was observed, and no statistical differences among monocultures were shown, getting higher concentration in combined culture. This was related to antihypertensive and antioxidant activities, where the highest values were also found in the combined process (45% of angiotensin-converting enzyme inhibition, and 168 µmol Trolox equivalents per liter [TE/L] for 2,2-diphenyl-1-picrylhydrazyl, 268 µmol TE/L for 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid, and 381 µmol Fe2E/L for ferric reducing ability of plasma). On the contrary, antithrombotic activity was not related to free amine groups during fermentation, having the highest bioactivity in different moments in each experiment. L. casei Shirota and S. thermophilus 54102 are strains that are able to release bioactive peptides from amaranth protein, although amaranth is not a common matrix for the development of lactic acid bacteria. In addition, in this study it was observed for the first time that lactic acid strains are able to release bioactive peptides from amaranth protein. In addition, this methodology could be part for the development of fermented beverages, different from fermented milk, to diversify matrix to obtain a novel functional food.


Asunto(s)
Amaranthus/química , Fermentación , Lactobacillales/metabolismo , Péptidos/farmacología , Proteínas de Plantas/farmacología , Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Antihipertensivos/farmacología , Antioxidantes/farmacología , Antitrombinas/farmacología , Ácido Láctico/metabolismo , Lactobacillus casei , Semillas/química , Streptococcus thermophilus
13.
Life Sci ; 229: 200-209, 2019 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-31047894

RESUMEN

AIMS: The rapeseed protein derived peptide DHNNPQIR (named as RAP-8) has been previously reported to possess antioxidant activity and alleviate liver fibrosis. The purpose of the present study was to investigate the potential crucial pathways involved in ameliorating liver fibrosis of RAP-8. MAIN METHODS: Next-generation sequencing of messenger RNA (RNA-Seq) analysis of the fibrotic and RAP-8 treated mice was performed. Western blot, qPCR and flow cytometry detection analysis were conducted to measure cell cycle and oxidative stress in LX-2 cells and liver samples. KEY FINDINGS: 588 overlapped differentially expressed genes were obtained from a batch of genes RAP-8 altered. Gene Ontology enrichment analysis revealed that changes in the most significant modules were mainly enriched in cell division, nuclear division and mitotic cell cycle process, while alterations in Kyoto Encyclopedia of Genes and Genomes were mainly enriched in cell cycle. Thereafter, according to the co-expression network analysis, the regulations of three core genes (Cenpp, Cyp2c55, Serpinh1) were verified that might be targets for treating liver fibrosis. Furthermore, through experimental verification, we demonstrated that RAP-8 induced cell cycle arrest and prevents oxidation stress. SIGNIFICANCE: As a promising therapeutic candidate for hepatic fibrosis treating, RAP-8 exhibited anti-fibrotic effects via exerting cell cycle arrest and inhibiting oxidative stress.


Asunto(s)
Brassica rapa/química , Ciclo Celular/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Cirrosis Hepática/prevención & control , Estrés Oxidativo/efectos de los fármacos , Fragmentos de Péptidos/farmacología , Proteínas de Plantas/farmacología , Animales , Perfilación de la Expresión Génica , Cirrosis Hepática/metabolismo , Cirrosis Hepática/patología , Masculino , Ratones , Ratones Endogámicos C57BL , ARN Mensajero/genética , ARN Mensajero/metabolismo
14.
J Nutr Sci Vitaminol (Tokyo) ; 65(2): 202-204, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31061291

RESUMEN

Rubiscolin-6 (Tyr-Pro-Leu-Asp-Leu-Phe) is produced by a pepsin digest of spinach d-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and known to act as an agonist on δ-opioid receptor. Here, we showed that administration of rubiscolin-6 reduced immobility time in the tail suspension test in restraint-stressed mice without effect on locomotor activity. The antidepressant-like effect of rubiscolin-6 was blocked by a δ-opioid receptor antagonist, naltrindole. These results indicate that rubiscolin-6 exerts antidepressant-like effect through activation of δ-opioid receptor.


Asunto(s)
Antidepresivos/farmacología , Fragmentos de Péptidos/farmacología , Proteínas de Plantas/farmacología , Ribulosa-Bifosfato Carboxilasa/farmacología , Spinacia oleracea , Estrés Psicológico , Animales , Conducta Animal/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos ICR , Restricción Física/efectos adversos , Spinacia oleracea/química , Spinacia oleracea/enzimología
15.
Food Funct ; 10(5): 2894-2905, 2019 May 22.
Artículo en Inglés | MEDLINE | ID: mdl-31070610

RESUMEN

Maca protein isolate (MPI) was extracted from maca root, and its physicochemical and functional properties, and the secondary structure and immunomodulatory activity of its major protein component, MMP, were investigated. The MPI lacked essential amino acids compared with soybean protein isolate (SPI) and casein, but was rich in cysteine and proline. The MPI had rich free sulfhydryl (20.6 µmol g-1), and its surface hydrophobicity (H0, 812.4), oil absorption capacity (7.4 g g-1), foaming capacity (100%) and emulsifying activity (58.2 m2 g-1) were higher than that of SPI. However, the thermal stability (Td, 87.4 °C), foaming stability (75%) and emulsifying stability (26.3 min) of the MPI were weaker than that of the SPI. MMP was a pentamer with a molecular weight of 22 kDa and rich in ß-sheets. MMP could significantly enhance the phagocytic capacity and promote the NO, TNF-α and IL-6 secretion of RAW 264.7 cells, involving toll-like receptor 4 and complement receptor 3 mainly.


Asunto(s)
Factores Inmunológicos/química , Lepidium/química , Proteínas de Plantas/química , Animales , Interacciones Hidrofóbicas e Hidrofílicas , Factores Inmunológicos/farmacología , Interleucina-6/genética , Interleucina-6/inmunología , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Ratones , Peso Molecular , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Estructura Secundaria de Proteína , Células RAW 264.7 , Receptor Toll-Like 4/genética , Receptor Toll-Like 4/inmunología , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/inmunología
16.
Mar Drugs ; 17(5)2019 May 11.
Artículo en Inglés | MEDLINE | ID: mdl-31083497

RESUMEN

Dexamethasone (DEX), a synthetic glucocorticoid, causes skeletal muscle atrophy. This study examined the protective effects of Pyropia yezoensis peptide (PYP15) against DEX-induced myotube atrophy and its association with insulin-like growth factor-I (IGF-I) and the Akt/mammalian target of rapamycin (mTOR)-forkhead box O (FoxO) signaling pathway. To elucidate the molecular mechanisms underlying the effects of PYP15 on DEX-induced myotube atrophy, C2C12 myotubes were treated for 24 h with 100 µM DEX in the presence or absence of 500 ng/mL PYP15. Cell viability assays revealed no PYP15 toxicity in C2C12 myotubes. PYP15 activated the insulin-like growth factor-I receptor (IGF-IR) and Akt-mTORC1 signaling pathway in DEX-induced myotube atrophy. In addition, PYP15 markedly downregulated the nuclear translocation of transcription factors FoxO1 and FoxO3a, and inhibited 20S proteasome activity. Furthermore, PYP15 inhibited the autophagy-lysosomal pathway in DEX-stimulated myotube atrophy. Our findings suggest that PYP15 treatment protected against myotube atrophy by regulating IGF-I and the Akt-mTORC1-FoxO signaling pathway in skeletal muscle. Therefore, PYP15 treatment appears to exert protective effects against skeletal muscle atrophy.


Asunto(s)
Dexametasona/toxicidad , Fibras Musculares Esqueléticas/efectos de los fármacos , Atrofia Muscular/tratamiento farmacológico , Péptidos/farmacología , Proteínas de Plantas/farmacología , Rhodophyta/química , Animales , Autofagia/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Dexametasona/farmacología , Proteínas Sustrato del Receptor de Insulina/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Lisosomas/metabolismo , Ratones , Fibras Musculares Esqueléticas/metabolismo , Fibras Musculares Esqueléticas/patología , Músculo Esquelético/efectos de los fármacos , Atrofia Muscular/inducido químicamente , Atrofia Muscular/metabolismo , Atrofia Muscular/patología , Péptidos/química , Proteínas de Plantas/química , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/efectos de los fármacos , Serina-Treonina Quinasas TOR/metabolismo
17.
Int J Oncol ; 54(6): 2080-2094, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31081059

RESUMEN

Amine oxidases, which contribute to the regulation of polyamine levels, catalyze the oxidative deamination of polyamines to generate H2O2 and aldehyde(s). In this study, and at least to the best of our knowledge, maize polyamine oxidase (ZmPAO) was used for the first time with the aim of identifying a novel strategy for cancer therapy. The cytotoxicity and the mechanisms of cell death induced by the enzymatic oxidation products of polyamine generated by ZmPAO were investigated. Exogenous spermine and ZmPAO treatment decreased cell viability in a spermine dose­ and time­dependent manner, particularly, the viability of the multidrug­resistant (MDR) colon adenocarcinoma cells, LoVo DX, when compared with drug­sensitive ones (LoVo WT). Further analyses revealed that H2O2 derived from spermine was mainly responsible for the cytotoxicity. Flow cytometric analysis revealed that treatment with ZmPAO and spermine increased the apoptotic population of LoVo WT and LoVo DX cells. In addition, we found that treatment with ZmPAO and spermine markedly reduced mitochondrial membrane potential in the LoVo DX cells, in agreement with the results of cell viability and apoptosis assays. Transmission electron microscopic observations supported the involvement of mitochondrial depolarization in the apoptotic process. Therefore, the dysregulation of polyamine metabolism in tumor cells may be a potential therapeutic target. In addition, the development of MDR tumor cells is recognized as a major obstacle in cancer therapy. Therefore, the design of a novel therapeutic strategy based on the use of this combination may be taken into account, making this approach attractive mainly in treating MDR cancer patients.


Asunto(s)
Adenocarcinoma/metabolismo , Neoplasias del Colon/metabolismo , Peróxido de Hidrógeno/metabolismo , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH/farmacología , Espermina/farmacología , Zea mays/enzimología , Adenocarcinoma/tratamiento farmacológico , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Neoplasias del Colon/tratamiento farmacológico , Relación Dosis-Respuesta a Droga , Resistencia a Antineoplásicos/efectos de los fármacos , Sinergismo Farmacológico , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Proteínas de Plantas/farmacología , Espermidina/farmacología , Factores de Tiempo
18.
Food Funct ; 10(6): 3466-3476, 2019 Jun 19.
Artículo en Inglés | MEDLINE | ID: mdl-31140514

RESUMEN

Pseudostellaria heterophylla has been becoming a popular research topic because of its functionally active components. The immunomodulatory activity of P. heterophylla peptide (PPH) derived from protein hydrolysate and the molecular mechanism underlying its immunomodulatory effect were investigated in this study. Immunomodulatory PPH achieved the highest stimulation index of 1.53 at a concentration of 100 µg mL-1 for 48 h in spleen lymphocytes and promoted the secretions of tumor necrosis factor-α, interferon-γ, and interleukin-10. Moreover, PPH could elevate the intracellular Ca2+ concentration, calcineurin activity and nuclear factor of activated T cell (NFAT) c1 mRNA expression. Meanwhile these effects could be diminished by the treatment of verapamil and cyclosporin A, suggesting that PPH may activate spleen lymphocytes via the Ca2+/CaN/NFATc1/IFN-γ signaling pathway. These findings demonstrate that the P. heterophylla peptide has the potential to be utilized as a nutraceutical supplement to strengthen the immune system in the human body.


Asunto(s)
Calcineurina/inmunología , Calcio/inmunología , Caryophyllaceae/química , Factores Inmunológicos/farmacología , Interferón gamma/inmunología , Linfocitos/efectos de los fármacos , Factores de Transcripción NFATC/inmunología , Péptidos/farmacología , Animales , Calcineurina/genética , Factores Inmunológicos/química , Interferón gamma/genética , Activación de Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Masculino , Ratones , Factores de Transcripción NFATC/genética , Péptidos/química , Proteínas de Plantas/química , Proteínas de Plantas/farmacología , Bazo/citología , Bazo/efectos de los fármacos , Bazo/inmunología
19.
Int J Biol Macromol ; 134: 791-797, 2019 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-31112732

RESUMEN

Lotus seed has long been used in traditional medicine and cuisine. In this study, lotus seed protein (LSP) was isolated and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LSP isolate (LSPI) treatment in LPS-stimulated RAW264.7 macrophages resulted in the significant (p < 0.05) decrease of NO production by downregulation of the expressions of mRNA and protein, including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, LSPI treatment attenuated the production of reactive oxygen species (ROS) through increasing catalase activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1ß in LPS-stimulated RAW264.7 macrophages. Furthermore, LPS stimulation in RAW264.7 macrophages caused the translocation of nuclear factor-kappa B (NF-κB) into the nucleus and the phosphorylation of mitogen-activated protein kinase (MAPK) but these stimulations were abolished by LSPI treatment.


Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Lotus/química , Macrófagos/efectos de los fármacos , Macrófagos/fisiología , Proteínas de Plantas/farmacología , Semillas/química , Transducción de Señal/efectos de los fármacos , Animales , Antiinflamatorios/química , Antioxidantes/química , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Mediadores de Inflamación/metabolismo , Lipopolisacáridos/inmunología , Activación de Macrófagos/efectos de los fármacos , Activación de Macrófagos/inmunología , Ratones , Proteínas Quinasas Activadas por Mitógenos , FN-kappa B/metabolismo , Fosforilación , Proteínas de Plantas/química , Células RAW 264.7 , Especies Reactivas de Oxígeno/metabolismo
20.
Appl Microbiol Biotechnol ; 103(14): 5533-5547, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31144014

RESUMEN

Potato proteins are well known for their nutritional, emulsifying, foaming, gel forming or antioxidant properties that all make from them valuable protein source for food industry. Antifungal, antimicrobial and also antiviral properties, described for potato proteins in the review, enrich the possibilities of potato protein usage. Potato proteins were divided into patatin, protease inhibitors and fraction of other proteins that also included, besides others, proteins involved in potato defence physiology. All these proteins groups provide proteins and peptides with antifungal and/or antimicrobial actions. Patatins, obtained from cultivars with resistance to Phytophthora infestans, were able to inhibit spore germination of this pathogen. Protease inhibitors represent the structurally heterogeneous group with broad range of antifungal and antimicrobial activities. Potato protease inhibitors I and II reduced the growth of Phytophthora infestans, Rhizoctonia solani and Botrytis cinerea or of the fungi of Fusarium genus. Members of Kunitz family (proteins Potide-G, AFP-J, Potamin-1 or PG-2) were able to inhibit serious pathogens such as Staphylococcus aureus, Listeria monocytogenes, Escherichia coli or Candida albicans. Potato snakins, defensins and pseudothionins are discussed for their ability to inhibit serious potato fungi as well as bacterial pathogens. Potato proteins with the ability to inhibit growth of pathogens were used for developing of pathogen-resistant transgenic plants for crop improvement. Incorporation of potato antifungal and antimicrobial proteins in feed and food products or food packages for elimination of hygienically risk pathogens brings new possibility of potato protein usage.


Asunto(s)
Antibacterianos/farmacología , Bacterias/efectos de los fármacos , Hongos/efectos de los fármacos , Fungicidas Industriales/farmacología , Proteínas de Plantas/farmacología , Solanum tuberosum/química , Antibacterianos/química , Antifúngicos/química , Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Hidrolasas de Éster Carboxílico/química , Hidrolasas de Éster Carboxílico/farmacología , Fungicidas Industriales/química , Listeria monocytogenes/efectos de los fármacos , Péptidos/química , Péptidos/farmacología , Phytophthora/efectos de los fármacos , Proteínas de Plantas/química , Staphylococcus aureus/efectos de los fármacos
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