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1.
Chin J Physiol ; 63(1): 50, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32056987

RESUMEN

[This corrects the article DOI: 10.4103/CJP.CJP_61_19].


Asunto(s)
Ginsenósidos , Animales , Glucosa , Ratas
2.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(2): 171-176, 2020 Feb.
Artículo en Chino | MEDLINE | ID: mdl-32051086

RESUMEN

OBJECTIVE: To study the role and mechanism of action of Huai Qi Huang (HQH) in the rat model of asthma. METHODS: Forty Sprague-Dawley rats were randomly divided into a control group, an asthma model group, a budesonide group, and an HQH group, with 10 rats in each group. A rat model of asthma was established by ovalbumin sensitization and challenge. The budesonide group was given budesonide aerosol 2 mg before each challenge. The HQH group was given HQH 4 g/kg dissolved in water by gavage before each challenge. Hematoxylin and eosin staining was used to observe the pathological changes of lung tissues. The percentage of eosinophils in bronchoalveolar lavage fluid (BALF) was measured. Enzyme-linked immunosorbent assay was used to determine the levels of interleukin-3 (IL-3), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), interferon gamma (INF-γ), and immunoglobulin E (IgE) in BALF. Flow cytometry was used to determine T-helper type 1 (Th1)/T-helper type 2 (Th2) ratio in peripheral blood and the spleen. RT-PCR and Western blot were used to measure the mRNA and protein expression of T-bet and GATA-3 in lung tissue. RESULTS: Compared with the control group, the asthma model group showed significant increases in the degree of airway inflammation, the percentage of eosinophils in BALF, and the levels of IL-3, IL-4, IL-5 and IgE in BALF (P<0.05), however, the asthma model group showed significant reductions in the levels of IL-10 and INF-γ in BALF (P<0.05). The asthma model group had significantly lower percentage of Th1 cells but significantly higher percentage of Th2 cells in peripheral blood and the spleen compared with the control group (P<0.05). The mRNA and protein expression of T-bet in lung tissue was significantly lower, but the mRNA and protein expression of GATA-3 in lung tissue was significantly higher in the asthma group than those in the control group (P<0.05). Both HQH and budesonide significantly improved airway inflammation and the above markers in asthmatic rats (P<0.05), with comparable effects between them. However, there were still significant differences in these indices between the control group and the HQH or budesonide group (P<0.05). CONCLUSIONS: HQH can reduce the airway inflammation of asthmatic rats and alleviate the symptoms of asthma, possibly by regulating the levels of related cytokines and Th1/Th2 ratio through the T-bet/GATA-3 pathway.


Asunto(s)
Asma , Animales , Líquido del Lavado Bronquioalveolar , Medicamentos Herbarios Chinos , Pulmón , Ratones Endogámicos BALB C , Ratas , Ratas Sprague-Dawley , Células Th2
3.
Eur J Histochem ; 64(1)2020 Jan 27.
Artículo en Inglés | MEDLINE | ID: mdl-31988532

RESUMEN

The goal of this study was to evaluate P450 aromatase localization in the epididymis of two different vertebrates: the lizard Podarcis sicula, a seasonal breeder, and Rattus rattus, a continuous breeder. P450 aromatase is a key enzyme involved in the local control of spermatogenesis and steroidogenesis and we proved for the first time that this enzyme is represented in the epididymis of both P. sicula and R. rattus. In details, P450 aromatase was well represented in epithelial and myoid cells and in the connective tissue of P. sicula epididymis during the reproductive period; instead, during autumnal resumption this enzyme was absent in the connective tissue. During the non-reproductive period, P450 aromatase was localized only in myoid cells of P. sicula epididymis, whereas in R. rattus it was localized both in myoid cells and connective tissue. Our findings, the first on the epididymis aromatase localization in the vertebrates, suggest a possible role of P450 aromatase in the control of male genital tract function, particularly in sperm maturation.


Asunto(s)
Aromatasa/fisiología , Epidídimo/enzimología , Animales , Tejido Conectivo/enzimología , Inmunohistoquímica , Lagartos , Masculino , Ratas , Reproducción/fisiología
4.
J Oral Sci ; 62(1): 13-17, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31996516

RESUMEN

Although xerostomia can cause persistent oral pain, the mechanisms underlying such pain are not well understood. To evaluate whether a phosphorylated p38 (pp38)-TRPV4 mechanism in trigeminal ganglion (TG) neurons has a role in mechanical hyperalgesia of dry tongue, a rat model of dry tongue was used to study the nocifensive reflex and pp38 and TRPV4 expression in TG neurons. The head-withdrawal reflex threshold for mechanical stimulation of the tongue was significantly lower in dry-tongue rats than in sham rats. The numbers of TRPV4- and pp38-immunoreactive cells in the TG were significantly higher in dry-tongue rats than in sham rats. Many TRPV4-IR cells were also pp38-immunoreactive. The number of TRPV1-IR cells was unchanged in the TG after induction of tongue dryness. Local injection of a TRPV4 blocker attenuated tongue mechanical hypersensitivity in dry-tongue rats. Intraganglionic injection of a selective p38 MAP kinase inhibitor eliminated tongue hypersensitivity in dry-tongue rats and suppressed TRPV4 expression in TG neurons. The present findings suggest that TRPV4 activation via p38 phosphorylation in TG neurons is involved in mechanical hypersensitivity associated with dry tongue. These mechanisms may have a role in pain associated with xerostomia.


Asunto(s)
Canales Catiónicos TRPV , Ganglio del Trigémino , Animales , Hiperalgesia , Ratas , Ratas Sprague-Dawley , Lengua
5.
J Oral Sci ; 62(1): 62-66, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31996525

RESUMEN

This study was performed to develop a new rat model of reduced masticatory activity in order to assess the effect of this reduction on the morphology of the temporomandibular joint (TMJ) over time. Female rats were used, and ovariectomy was performed to simulate aged/postmenopausal status. Twenty-four SD rats aged 6 weeks were divided into four groups: ovariectomy/sham procedure (Ov/S); ovariectomy/reduced masticatory activity (Ov/RMA); non-Ov/S (NO/S); and non-Ov/RMA (NO/RMA). The RMA procedure involved grinding down the edges of the upper and mandibular incisors by about 3 mm and supplying the rats with a powdered diet. The bilateral TMJ was examined by micro-computed tomography at 0, 1, 2, 4, 6, and 8 weeks after the start of RMA. Condylar width was greater in the NO/S group than in the Ov/S group after the 2nd week, showing that ovariectomy reduced the width of the condyle. After the 2nd week, significant differences in condylar width were apparent between the NO/S and NO/RMA groups, and between the Ov/S and Ov/RMA groups. This RMA procedure appeared to provide a good model of reduced masticatory activity. The present findings in female rats suggest that reduction of appropriate mastication activity in the growth period results in poor growth of the mandibular condyle and immediately induces atrophy of the mandibular condyle under conditions simulating aged/postmenopausal status.


Asunto(s)
Cóndilo Mandibular , Masticación , Animales , Atrofia , Femenino , Modelos Animales , Ratas , Ratas Sprague-Dawley , Microtomografía por Rayos X
6.
J Oral Sci ; 62(1): 70-74, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31996527

RESUMEN

The aim of this study was to establish an experimental rat model of temporomandibular joint (TMJ) anterior disc displacement (ADD). A pilot study was conducted to determine the most appropriate surgical protocol. In the main experiment, 40 rats were used. Twenty-four rats were subjected to ADD in the right TMJ, and subsequently thereafter six, nine, and nine rats were sacrificed at 1, 4, and 8 weeks, respectively, for gross evaluation. Twelve rats that underwent a sham operation were equally divided and sacrificed at each of the above time points. Four non-treated control rats were sacrificed at the beginning of the study. TMJ blocks were harvested for radiological and histological assessment. Gross examination showed that 14 rats in the ADD group (58.3%) had anterior displacement of the TMJ disc. In the ADD joints, posterior condylar cartilage thickness decreased during the follow-up period; however, there was no significant difference between the sham-treated and ADD joints, or among the follow-up time points (P > 0.05). The anterior condylar cartilage exhibited obvious qualitative alterations. Radiologic signs of osteoarthrosis appeared after ADD surgery, but this became attenuated with time. The model investigated in this study successfully induced ADD in rats, and should be useful for assessment of progressive changes in the TMJ following ADD.


Asunto(s)
Luxaciones Articulares , Trastornos de la Articulación Temporomandibular , Animales , Cóndilo Mandibular , Proyectos Piloto , Ratas , Articulación Temporomandibular , Disco de la Articulación Temporomandibular
7.
Chem Commun (Camb) ; 56(7): 1085-1088, 2020 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-31894779

RESUMEN

We report an elastase-responsive, H2S-releasing hydrogel prepared by covalently crosslinking a mixture of carboxymethylcellulose and poly(ethylene glycol) with an elastase-degradable peptide functionalized with an H2S-releasing S-aroylthiooxime (SATO) unit. Addition of elastase triggered a gel-to-sol transition, which exposed SATOs, leading to more and longer H2S release compared to untriggered gels.


Asunto(s)
Carboximetilcelulosa de Sodio/farmacología , Hidrogeles/farmacología , Sulfuro de Hidrógeno/metabolismo , Elastasa de Leucocito/metabolismo , Polietilenglicoles/farmacología , Animales , Carboximetilcelulosa de Sodio/síntesis química , Carboximetilcelulosa de Sodio/metabolismo , Línea Celular , Doxorrubicina/toxicidad , Humanos , Hidrogeles/síntesis química , Hidrogeles/metabolismo , Estrés Oxidativo/efectos de los fármacos , Oximas/síntesis química , Oximas/metabolismo , Oximas/farmacología , Polietilenglicoles/síntesis química , Polietilenglicoles/metabolismo , Sustancias Protectoras/síntesis química , Sustancias Protectoras/metabolismo , Sustancias Protectoras/farmacología , Ratas
8.
Bratisl Lek Listy ; 121(1): 8-13, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31950834

RESUMEN

OBJECTIVES: The neural stem cell transplantation has been proposed as alternative therapy to promote functional recovery after various neurological disorders. The aim of this study was to evaluate the effect of intra-arterial transplantation of adult neural stem cells on improving local brain ischemia injuries. MATERIALS AND METHODS: In this study, 32 male Wistar rats were used. Ischemia was induced using a middle cerebral artery obstruction with monofilament nylon suture. Neural stem cells were isolated from subventricular zone of the rat brain. 24 hours after local ischemia, the cells were labeled with DiI and transplanted intra-articularly. Evaluation of neurological movement deficits was performed using a neurological deficit score. The transplanted neural stem cells differentiation into neurons and astrocytes was assessed by immunohistochemistry. RESULTS: The results indicated that lesion volumes in the ischemic control, PBS and the treatment groups were 31.5, 29.8 and 14.7 % respectively. Our results also showed that the number of eosinophilic neurons and also neurological impairment in the treatment group was significantly reduced compared to the control and PBS groups. CONCLUSION: The results suggested that intra-arterial transplantation of neural stem cells 24 hours after ischemia, led to a decrease in the volume of brain ischemic lesion and improved neurological outcomes (Fig. 7, Ref. 23).


Asunto(s)
Isquemia Encefálica , Células-Madre Neurales , Trasplante de Células Madre , Accidente Cerebrovascular , Animales , Isquemia Encefálica/terapia , Diferenciación Celular , Masculino , Ratas , Ratas Wistar , Recuperación de la Función , Accidente Cerebrovascular/terapia
9.
Bratisl Lek Listy ; 121(1): 14-21, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31950835

RESUMEN

AIM: Apelin is an active endogenous peptide, which affects blood vessels. Also exercise increases angiogenesis after myocardial infarction and exerts cardio protective effects. The aim of the present study was to investigate the effect of Apelin and aerobic exercise on reducing the severity of Ischemia-reperfusion injury in rats. METHODS: The rats were divided into the following 4 groups 8 weeks before surgery (Langendorff model of perfusion): I) Ischemia-reperfusion (I/R), II: Exercise Ischemia- reperfusion (EX+I/R), III: Apelin+Ischemia-reperfusion (APE+I/R) (Apelin 10 nmol/kg/day, i.p), and IV: Exercise+Apelin+Ischemia-reperfusion (EX+APE+I/R). Exercise was performed on a treadmill 8 weeks before the surgery at a speed of 17 m/min for 10 to 50 min/day. The ventricular function was evaluated after I/R injury, histopathological and immunohistopathology indices were then measured at the scar tissue. RESULTS: The results of H(et)E, Masson's trichrome staining indicated that APE+EX pre-treatment reduced cardiac fibrosis and the percentage of collagen deposition. It also enhanced the microvessels density (MVD) and decreased the number of inflammatory cells and apoptosis rate. CONCLUSION: According to our study, Apelin and exercise preconditioning had anti-fibrotic and anti-apoptotic effects on the ischemia-reperfusion myocardium cells, which could lead to the protection of cardiac cells (Tab. 5, Fig. 3, Ref. 33).


Asunto(s)
Apelina , Infarto del Miocardio , Daño por Reperfusión , Animales , Apelina/farmacología , Apelina/uso terapéutico , Péptidos y Proteínas de Señalización Intercelular , Infarto del Miocardio/complicaciones , Miocitos Cardíacos , Ratas , Daño por Reperfusión/prevención & control , Daño por Reperfusión/terapia
10.
Bratisl Lek Listy ; 121(1): 37-42, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31950838

RESUMEN

OBJECTIVE: Hemopressin (Hp) is the first peptide ligand described for the CB1 cannabinoid receptor. Therefore, we aimed to investigate the effect of hemopressin on pencillin-induced epileptiform activity by using electrophysiological recording (ECoG) technique. METHODS: Male Wistar rats were anesthetized with urethane (1.25 g/kg), and epileptiform activity was induced by intracortical injection of penicillin (500 IU). Animals were randomly divided into eight groups. Subsequently, the rats were administered with saline or hemopressin as follows: saline control group (Group I: 2 µl/i.c.v/saline), hemopressin groups (Group II: 0.025 µg/i.c.v; Group III: 0.075 µg/i.c.v; Group IV: 0.15 µg/i.c.v; Group V: 0.3 µg/i.c.v; Group VI: 0.6 µg/i.c.v; Group VII: 1.2 µg/i.c.v; Group VIII: 2.4 µg/i.c.v). The various doses of hemopressin were injected intracerebroventricularly (i.c.v) 30 minutes after penicillin (2.5µl) injection. After hemopressin injection, ECoGs were recorded for three hours. RESULTS: Hp at doses of 0.075, 0.15, 0.3, 0.6, 1.2 and 2.4 µg/kg significantly increased the frequency of epileptiform ECoG activity compared to penicillin-injected group without changing the amplitude. The 0.6 µg hemopressin was the most effective dose to increase the epileptiform activity (p 0.05). CONCLUSIONS: The results of this study provided electrophysiological evidence for hemopressin to be modulating penicillin-induced epileptiform activity by acting as CB1 receptor antagonist. Further studies are required to elucidate the involved mechanism underlying this effect (Fig. 3, Ref. 40).


Asunto(s)
Epilepsia , Hemoglobinas , Penicilinas , Fragmentos de Péptidos , Animales , Epilepsia/inducido químicamente , Hemoglobinas/farmacología , Masculino , Penicilinas/efectos adversos , Fragmentos de Péptidos/farmacología , Distribución Aleatoria , Ratas , Ratas Wistar
11.
Bratisl Lek Listy ; 121(1): 62-66, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31950841

RESUMEN

AIM: The aim of this study was to determine the effect of desflurane on reproductive capacity in female rats through a study of biochemical evaluations. METHOD: After experimental procedure, the blood samples of female rats were collected, and the malondialdehyde, interleukin­1­beta, total glutathione and superoxide dismutase levels were measured to evaluate oxidative stress. In addition to biochemical evaluations, the reproductive performance of the experimental groups was also examined. RESULTS: The results of our study demonstrated that in blood samples of desflurane­treated groups of rats, the parameters indicating oxidative stress and inflammation increased, and antioxidant parameters decreased (p < 0.05). It was also proven that repeated desflurane doses caused infertility in female rats, prolonged the gestation period and reduced the number of offspring. CONCLUSIONS: This study showed that recurrent desflurane application can cause infertility problems through oxidative stress in female rats (Tab. 3, Fig. 1, Ref. 25).


Asunto(s)
Desflurano , Infertilidad Femenina , Estrés Oxidativo , Animales , Antioxidantes , Desflurano/toxicidad , Femenino , Glutatión Peroxidasa , Infertilidad Femenina/inducido químicamente , Malondialdehído , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Wistar , Superóxido Dismutasa
12.
Bratisl Lek Listy ; 121(1): 73-78, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31950843

RESUMEN

AIM: The aim of the present study was to investigate the immunohistochemical expression of selected collagen types, namely collagen types I and V and procollagen type III in the renal parenchyma and interstitium and in the myocardium of spontaneously hypertensive rats. MATERIAL AND METHODS: For the present study, we used two age groups of 6- and 12-month-old spontaneously hypertensive rats. An immunohistochemical analysis was conducted with monoclonal antibodies against collagen types I and V and procollagen type III. A semi-quantitative analysis of immunostaining intensity was conducted with the Image J software. RESULTS: In the kidney, all three molecules showed higher expression at the age of 12 months, which was particularly notable for procollagen type III and collagen type V, which stained as highly-positive. In the myocardium, the immunoreactivity of collagen types I and V was stronger in 12-month-old animals, while that of procollagen type III did not change substantially. CONCLUSION: The present study suggests a role of collagen types III and V in hypertensive kidney disease, while also establishing the role of increased expression of collagen types I and V in adverse myocardial remodeling (Tab. 1, Fig. 2, Ref. 48).


Asunto(s)
Hipertensión , Riñón , Miocardio , Animales , Colágeno/metabolismo , Corazón , Hipertensión/metabolismo , Hipertensión/fisiopatología , Riñón/metabolismo , Miocardio/metabolismo , Ratas , Ratas Endogámicas SHR , Ratas Endogámicas WKY , Remodelación Vascular
13.
Life Sci ; 242: 117211, 2020 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-31891720

RESUMEN

Ventricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. Our goal was to prevent and regress the hypertrophy by the activation of GPER in neonatal cardiac myocytes (NRCM) and SHR male rats. Aldosterone increased the neonatal cardiomyocytes cell surface area after 48 h of incubation. The aldo-induced hypertrophy was blocked by the mineralocorticoid receptor (MR) inhibitor Eplererone or the reduction of MR expression by siRNA. The activation of GPER by the agonist G-1 totally prevented the increase surface area by Ald. The transfection of neonatal rat cardiac myocytes with a siRNA against GPER or the incubation with GPER blockers G-15 and G-36 inhibited the protection of G-1. The significant increase of cell surface area after 48 h of incubation with Ald was totally regressed in 24 h by the presence of G-1, indicating that the activation of GPER not only prevent the hypertrophy but also regress the hypertrophy when it is already established. In the in vivo model, G-1 or Vehicle was constantly infused via the minipump to SHR. The reduction of the hypertrophy by G-1 was evident by the cross-sectional area, BNP and ANP markers and by echocardiography. In this studied we demonstrated that the activation of GPER prevented and regressed the hypertrophy induced by Ald in NRCM and regressed hypertrophy in SHR rats.


Asunto(s)
Cardiomegalia/prevención & control , Receptores Acoplados a Proteínas G/metabolismo , Animales , Animales Recién Nacidos , Western Blotting , Cardiomegalia/diagnóstico por imagen , Células Cultivadas , Ciclopentanos/farmacología , Ecocardiografía , Eplerenona/farmacología , Masculino , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , Quinolinas/farmacología , Ratas , Ratas Endogámicas SHR , Ratas Wistar , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores Acoplados a Proteínas G/antagonistas & inhibidores , Receptores Acoplados a Proteínas G/fisiología
14.
Life Sci ; 244: 117306, 2020 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-31953159

RESUMEN

AIMS: Accumulated evidence indicates that the dysregulation of circular RNAs (circRNAs) plays pivotal roles in many human diseases including preeclampsia (PE). Circ_0063517 has been verified to be down-regulated in PE. But the role of circ_0063517 in PE is still unclear. This research aims to probe into the effect of circ_0063517 on angiogenesis in PE development. MAIN METHODS: The expression of circ_0063517, endothelin receptor type B (ETBR) and miR-31-5p was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). MTT assay, colony formation assay, scratch assay, transwell assay, and tube formation assay were performed to detect proliferation, migration, and angiogenesis, respectively. Dual luciferase reporter system and RNA immunoprecipitation (RIP) assay were carried out to determine the interaction between miR-31-5p and circ_0063517(or ETBR). ETBR, VEGFRA, and VEGFR2 levels were detected by western blot analysis. The effect of circ_0063517 and ETBR on angiogenesis was evaluated in N-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced PE in vivo. KEY FINDINGS: The levels of circ_0063517 and ETBR were down-regulated in the placenta tissue of PE patients. Conversely, the level of miR-31-5p was up-regulated in PE. Overexpression of circ_0063517 or knockdown of miR-31-5p facilitated growth, migration, and angiogenesis of vascular endothelial cells. Circ_0063517 knockdown-induced repression of the expression of ETBR, VEGFA, and VEGFR2 was partly counteracted by ETBR overexpression. Mechanistically, circ_0063517 sponged miR-31-5p to regulate ETBR expression. Finally, circ_0063517 promoted angiogenesis via enhancing ETBR expression in PE in vivo. SIGNIFICANCE: Our findings suggest that circ_0063517-miR-31-5p-ETBR axis regulates angiogenesis during the pathological process of PE.


Asunto(s)
MicroARNs/metabolismo , Preeclampsia/metabolismo , Receptor de Endotelina B/metabolismo , Animales , Línea Celular , Proliferación Celular/fisiología , Células Endoteliales/metabolismo , Femenino , Células Endoteliales de la Vena Umbilical Humana , Humanos , MicroARNs/genética , Neovascularización Patológica/genética , Preeclampsia/genética , Embarazo , Ratas , Ratas Sprague-Dawley
15.
Life Sci ; 244: 117324, 2020 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-31958420

RESUMEN

AIMS: The aim of the present study was to evaluate the possible antioxidant role of oleic acid (OA) against Cd-induced injuries in the heart and liver tissues of male Wistar rats. MAIN METHODS: Rats were treated with either vehicle (control), or OA (10 mg/kg b.w., fed orally), or Cd (0.44 mg/kg b.w., s.c.), or both (OA + Cd) for 15 days. Following completion of the treatment period, biomarkers of organ damage and oxidative stress including ROS, activities of antioxidant enzymes and their level, activities of Krebs cycle enzymes and respiratory chain enzymes were measured. Levels of interleukins (IL-1ß, IL-6, IL-10), tumor necrosis factor (TNF-α) and nuclear factor kappa B (NFκB) were estimated to evaluate the state of inflammation. In addition, changes in mitochondrial membrane potential and status of cytochrome c (Cyt c) were also studied. KEY FINDINGS: Pre-treatment of rats with OA significantly protected against Cd-induced detrimental changes possibly by decreasing endogenous ROS through regulation of antioxidant defense system, inflammatory responses and activities of metabolic enzymes. Moreover, OA was also found to restore mitochondrial membrane potential possibly by regulating Cyt c leakage thereby increasing mitochondrial viability. SIGNIFICANCE: Our results for the first time demonstrated systematically that OA provided protection against Cd-induced oxidative stress mediated injuries in rat heart and liver tissues through its antioxidant mechanism. The results raise the possibility of using OA singly or in combination with other antioxidants or diet in the treatment of situations arising due to oxidative stress and may have future therapeutic relevance.


Asunto(s)
Ácido Oléico/metabolismo , Ácido Oléico/farmacología , Animales , Antioxidantes/farmacología , Biomarcadores/metabolismo , Cadmio/efectos adversos , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Corazón/efectos de los fármacos , Lesiones Cardíacas/prevención & control , Inflamación/patología , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Miocardio/metabolismo , Miocardio/patología , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Wistar
16.
Zhongguo Zhen Jiu ; 40(1): 59-66, 2020 Jan 12.
Artículo en Chino | MEDLINE | ID: mdl-31930901

RESUMEN

OBJECTIVE: To explore the mechanism of catgut embedding at back-shu points on nonalcoholic steatohepatitis (NASH) in rats based on IKK/IKB/NF-κB signaling pathway and downstream inflammatory factors. METHODS: Eighty SPF SD rats were selected, among them 10 rats were selected divided into a normal group (group A), and the remaining 70 rats were fed with high-fat diet to establish NASH model. At the end of 12 weeks, 10 rats were randomly selected to verify whether the model establishment was successful. Then the remaining 60 rats were randomly divided into a model group (group B), a catgut embedding at back-shu points group (group C), a catgut embedding at abdominal points group (group D), an acupuncture at back-shu points group (group E), a sham catgut embedding group (group F) and a western medication group (group G), 10 rats in each group. The rats in the group C were treated with catgut embedding at "Ganshu" (BL 18), "Pishu" (BL 20), "Weishu" (BL 21) and "Shenshu" (BL 23); the rats in the group D were treated with catgut embedding at "Daheng" (SP 15), "Fujie" (SP 14), "Huaroumen" (ST 24) and "Tianshu" (ST 25); the rats in the group E were treated with acupuncture at the same acupoints as the group C; the rats in the group F were treated with catgut embedding at back-shu points but the needle did not enter subcutaneous tissue gamma; the rats in the group G were treated with intragastric administration of vitamin E capsule. All the treatment was given for 4 weeks. The rats in the group A were fed with normal diet until the end of 16 weeks without any intervention. The rats in the group B continued to be fed with high-fat diet until the end of 16 weeks. After the intervention, the liver index was calculated; the liver histomorphology was observed by HE staining; the liver function [alanine aminotransferase (ALT), gamma glutamyl transferase (γ-GGT), alkaline phosphatase (ALP)] and blood lipid [serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL)] were measured by serum biochemistry. The serum levels of TNF-α, IL-6 and IL-1ßwere detected by ELISA, and the expressions of IKK-α, NF-κBp65, IL-6, IL-1ß and TNF-α proteins in liver tissue were detected by Western blot. The temperature of the conception vessel and the governor vessel was measured by infrared thermography. RESULTS: Compared with the group A, the obvious steatosis and inflammatory cell infiltration were observed in the group B, and the body weight, liver wet-weight and liver index were all increased (P<0.01). Compared with the group B, the liver tissue morphology in the group C, the group D, the group E and the group G was improved in varying degrees, and the liver index was decreased (P<0.05), which was the most significant in the group C (P<0.05). Compared with the group A, the ALT, γ-GGT, ALP, TG, TC, LDL, TNF-α, IL-6 and IL-1ß were all increased in the group B (P<0.01); compared with the group B, the ALT, γ-GGT, ALP, TG, TC, LDL, TNF-α, IL-6 and IL-1ß in all intervention groups were all decreased in varying degrees (P<0.01, P<0.05), which was the most significant in the group C (P<0.01). Compare with the group A, the expressions of IKK-α, NF-κBp65, TNF-α, IL-6 and IL-1ßproteins in the group B were all increased (P<0.01); compared with the group B, the expressions of IKK-α, NF-κBp65, TNF-α, IL-6 and IL-1ßproteins in all intervention groups were decreased in varying degrees (P<0.05), which was the most significant in the group C (P<0.01). Compared with the group A, the temperature of the conception vessel and governor vessel was decreased in the group B (P<0.01). Compared with the group B, the temperature of the conception vessel and governor vessel was all increased in the group C, the group D and the group E (P<0.01); the temperature of the conception vessel in the group C was similar to that in the group D (P>0.05), while the temperature of the governor vessel in the group C was superior to that in the group D (P<0.05). CONCLUSION: The catgut embedding at back-shu points might inhibit the activation of IKK/IKB/NF-κB signaling pathway to interrupt the inflammatory cascade, and reduce the "second hit" of inflammatory factors on liver, which could slow down NASH progress and prevent and treat NASH.


Asunto(s)
Catgut , Enfermedad del Hígado Graso no Alcohólico , Puntos de Acupuntura , Animales , FN-kappa B , Ratas , Ratas Sprague-Dawley , Transducción de Señal
17.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(1): 102-108, 2020 Jan 15.
Artículo en Chino | MEDLINE | ID: mdl-31939244

RESUMEN

Objective: To evaluate the feasibility of the chitosan-poly (lactide-co-glycolide) (PLGA) double-walled microspheres for sustained release of bioactive nerve growth factor (NGF) in vitro. Methods: NGF loaded chitosan-PLGA double-walled microspheres were prepared by emulsion-ionic method with sodium tripolyphosphate (TPP) as an ionic cross-linker. The double-walled microspheres were cross-linked by different concentrations of TPP [1%, 3%, 10% ( W/ V)]. NGF loaded PLGA microspheres were also prepared. The outer and inner structures of double-walled microspheres were observed by light microscopy, scanning electron microscopy, confocal laser scanning microscopy, respectively. The size and distribution of microspheres and fourier transform infra red spectroscopy (FT-IR) were analyzed. PLGA microspheres with NGF or chitosan-PLGA double-walled microspheres cross-linked by 1%, 3%, and 10%TPP concentration (set as groups A, B, C, and D respectively) were used to determine the degradation ratio of microspheres in vitro and the sustained release ratio of NGF in microspheres at different time points. The bioactivity of NGF (expressed as the percentage of PC12 cells with positive axonal elongation reaction) in the sustained release solution of chitosan-PLGA double-walled microspheres without NGF (set as group A1) was compared in groups B, C, and D. Results: The chitosan-PLGA double-walled microspheres showed relative rough and spherical surfaces without aggregation. Confocal laser scanning microscopy showed PLGA microspheres were evenly uniformly distributed in the chitosan-PLGA double-walled microspheres. The particle size of microspheres ranged from 18.5 to 42.7 µm. The results of FT-IR analysis showed ionic interaction between amino groups and phosphoric groups of chitosan in double-walled microspheres and TPP. In vitro degradation ratio analysis showed that the degradation ratio of double-walled microspheres in groups B, C, and D appeared faster in contrast to that in group A. In addition, the degradation ratio of double-walled microsphere in groups B, C, and D decreased when the TPP concentration increased. There were significant differences in the degradation ratio of each group ( P<0.05). In vitro sustained release ratio of NGF showed that when compared with PLGA microspheres in group A, double-walled microspheres in groups B, C, and D released NGF at a relatively slow rate, and the sustained release ratio decreased with the increase of TPP concentration. Except for 84 days, there was significant difference in the sustained release ratio of NGF between groups B, C, and D ( P<0.05). The bioactivity of NGF results showed that the percentage of PC12 cells with positive axonal elongation reaction in groups B, C, and D was significantly higher than that in group A1 ( P<0.05). At 7 and 28 days of culture, there was no significant difference between groups B, C, and D ( P>0.05); at 56 and 84 days of culture, the percentage of PC12 cells with positive axonal elongation reaction in groups C and D was significantly higher than that in group B ( P<0.05), and there was no significant difference between groups C and D ( P>0.05). Conclusion: NGF loaded chitosan-PLGA double-walled microspheres have a potential clinical application in peripheral nerve regeneration after injury.


Asunto(s)
Microesferas , Animales , Quitosano , Dioxanos , Factor de Crecimiento Nervioso , Tamaño de la Partícula , Ratas , Espectroscopía Infrarroja por Transformada de Fourier
18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(1): 7-12, 2020 Jan.
Artículo en Chino | MEDLINE | ID: mdl-31950782

RESUMEN

Objective: To study the effects of genistein (GEN) on reproductive system in prepubertal male rats. Methods: Thirty SPF-rated male SD rats were randomly divided into control group (Con group), low-dose group (G1 group) and high-dose group (G2 group), with 10 rats in each group. Corn oil, 150 mg/kg and 300 mg/kg GEN dissolved in corn oil of equal volume were respectively administered every day and weighed the next day. After 6 weeks, the rats were sacrificed, and the testis, epididymis and prostate were dissected, and organ coefficients were calculated. Histopathological changes of testis was observed. The number of sperm was counted and the rate of sperm malformation was calculated. The concentrations of serum testosterone and estradiol were detected by radioimmunoassay. The protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C) protein expression in testicular tissue was detected by immunofluorescence assay. The mRNA and protein expression levels of PPP2R2C and cyclin dependent protein kinases 2 (CDK2) in rat testis were detected by real-time quantitative fluorescence PCR (RT-qPCR) and Western blot, respectively. The protein phosphatase 2A (PP2A) activity in testicular tissue was detected by immunoprecipitation. Results: There were no statistically significant differences in body mass, sperm number, serum estradiol and PP2A enzyme activity among the groups ( P>0.05). The pathological structure of testicular in G2 group was disordered. Sperm abnormality rate in G1 and G2 groups was higher than that in Con group ( P<0.05). Serum testosterone concentration in G2 group was lower than that in Con group ( P<0.05). The expression of PPP2R2C and CDK2 in G2 group was higher than that in Con group ( P<0.05), but the protein level was lower than that in Con group ( P<0.05). PPP2R2C protein was expressed in testicular tissue in each group. Conclusion: Long-term exposure to high dose (300 mg/kg) GEN during prepuberty may cause adverse effects on reproductive function in adult male rats. Further investigation is needed to determine whether PPP2R2C-PP2A-CDK2 phosphorylation pathway affects reproductive system in rats.


Asunto(s)
Genisteína , Genitales Masculinos , Animales , Estradiol/sangre , Regulación de la Expresión Génica/efectos de los fármacos , Genisteína/farmacología , Genitales Masculinos/efectos de los fármacos , Masculino , Fitoestrógenos/farmacología , Ratas , Ratas Sprague-Dawley , Recuento de Espermatozoides , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Testículo/enzimología , Testosterona/sangre
19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(1): 13-17, 2020 Jan.
Artículo en Chino | MEDLINE | ID: mdl-31950783

RESUMEN

Objective: To investigate the effects of Akkermansia muciniphila ( A. muciniphila) on the proliferation, apoptosis and insulin secretion of rat pancreatic islet cell tumor cells (INS-1). Methods: INS-1 cells were divided into three groups, normal, repair, and protect groups, and subsequently every group was subjected with A. muciniphila metabolites, live A. muciniphilaorpasteurized A. muciniphila for 48 h. A group that did not treat with anything was set as blank control. After intervention, the cell viability was determined by MTT method, the insulin secretion level stimulated by glucose was determined by ELISA, the expressions of the genes involved in insulin secretion and apoptosis were tested by qRT-PCR, and the expression of apoptosis related protein Bax was evaluated by Western blot. Results: There was no significant change in INS-1 cell morphology after co-incubation with 3 types of A. Muciniphila interventions for 48 h. The proliferative activity of INS-1 cells was decreased in the repair group that treated with live A. muciniphila than that of control ( P<0.005). A. muciniphila intervention had no effect on insulin secretion in INS-1 cells in normal, repair or protection group ( P>0.05). A. muciniphila secretions promoted the expression of glucose transporter 2 ( Glut2) in 3 groups and the expression of glucokinase ( GCK) in repair group ( P<0.05). The expression of Baxof the INS-1 cell in the normal group was decreased after intervented with 3 kinds of A. muciniphila intervention materials ( P<0.001).The expression of Bax gene of the INS-1 cell in the repair group that treated with dead A. muciniphilawas decreased ( P<0.05). The expression of Bax protein of INS-1 cells that treated with A. muciniphila interventions was decreased. Conclusion: A. muciniphila can promote the expression of insulin secretion-related genes in INS-1 cells, inhibit the expression of apoptotic genes and apoptosis protein Bax.This research provides a new direction for applying A. muciniphila in improving type 2 diabetes.


Asunto(s)
Adenoma de Células de los Islotes Pancreáticos , Apoptosis , Diabetes Mellitus Tipo 2 , Secreción de Insulina , Probióticos , Verrucomicrobia , Animales , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular , Regulación de la Expresión Génica/efectos de los fármacos , Glucosa/farmacología , Secreción de Insulina/efectos de los fármacos , Ratas , Verrucomicrobia/fisiología
20.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(1): 67-73, 2020 Jan.
Artículo en Chino | MEDLINE | ID: mdl-31950792

RESUMEN

Objective: To develop and verify a flow cytometric measurement of reticulocytes (RETs) micronucleus in rat bone marrow. Methods: In our flow cytometric protocol, reticulocytes, leukocytes and DNA were labeled by anti-CD71-fluorescein isothiocyanate (FITC), anti-CD45-phycoerythrin (PE) and DRAQ5, respectively. Sprague-Dawley (SD) rats were assigned to four treatment groups randomly, and were exposed to ethyl methanesulfonate (EMS), cyclophosphamide (CP), ethyl nitrosourea (ENU) and colchicine (COL) respectively. Each treatment group was divided into four subgroups (5 rats per subgroup) according to different exposure dosage. A exposure dose of 0 was used as vehicle control for each group. Rats were administered with testing mutagens by gavage twice with a 24 h interval. Bone marrow from both femurs were collected 24 h after the last administration. The frequency of micronucleated reticulocytes (MN-RETs) and the percentage of reticulocytes (RETs%) were determined by flow cytometric measurement established in this study. And the manual counting method with microscope (by Giemsa staining) was conducted at the same time. Results: A method for detection of reticulocyte micronucleus in bone marrow based on flow cytometry was successfully established. The MN-RETs in rat bone marrow of 20 SD rats treated by vehicle (i.e., background value of MN-RETs) was 0.83‰±0.12‰ by this method. The background value of MN-RETs in manual enumeration method was 1.43‰±0.44‰. It was obvious that the flow cytometric method had lower background value and more stable results. The trend, in which MN-RETs ascended and RETs% descended with increasing dose, can be detected by both methods in rats that exposed to EMS, CP, ENU and COL. Both methods were good to detect the correlation of induced-MN-RETs with four testing mutagens (the correlation coefficients were ranged from 0.834 3 to 0.913 7). Conclusion: With its sensitivity, rapidity, easy operation and low background value, the three-color flow cytometric enumerative protocol established in our laboratory can be used as a good substitute for manual micronucleus counting method and used in genotoxicity assessment of chemical substances.


Asunto(s)
Médula Ósea , Citometría de Flujo , Reticulocitos , Animales , Pruebas de Micronúcleos , Ratas , Ratas Sprague-Dawley , Reticulocitos/citología
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