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1.
Oral Health Prev Dent ; 18(2): 311-318, 2020 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-32618454

RESUMEN

PURPOSE: The aim of this study was to investigate the in vitro effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride-containing varnish on prevention enamel erosive tooth wear. MATERIALS AND METHODS: A total of 28 enamel samples were prepared from human molars, divided into four groups: CPP-ACPF varnish, TCP-F varnish, NaF varnish, and deionised water. For the remineralisation process stimulated human pooled saliva was used. After treatment, all enamel samples were exposed to 10 ml of Coca Cola. Ca++ release was determined by atomic absorption spectroscopy (AAS). The surface topography was evaluated by atomic force microscopy (AFM). Surface microhardness of enamel was analysed and SMHR % (surface microhardness reduction ) was calculated. Data were analysed with repeated measures analysis of variance (ANOVA). RESULTS: Deionised water demonstrated a statistically significantly higher Ca+2 release compared to those of groups NaF > fTCP > CPP-ACPF, respectively (p <0.01). All groups measured for root-mean-square-roughness (Rrms) showed a statistically significantly difference of 6 × 6 µm2 and 12 × 12 µm2 enamel area (p <0.05) compared with a negative control group. CPP-ACPF varnish showed rougher surfaces than all remineralisation groups. SMHR % of enamels were as follows: CPP-ACPF < fTCP < NaF < deionised water (p <0.01). CONCLUSION: According to the findings of this study; CPP-ACP containing agents have a statistically statistically significant effect on preventing dental erosion. Among these, CPP-ACPF-containing remineralisation agents have the most effect on the remineralisation process.


Asunto(s)
Esmalte Dental , Desgaste de los Dientes , Caseínas , Humanos , Microscopía de Fuerza Atómica , Pintura , Pirazinas , Remineralización Dental
2.
Shanghai Kou Qiang Yi Xue ; 29(1): 46-50, 2020 Feb.
Artículo en Chino | MEDLINE | ID: mdl-32524120

RESUMEN

PURPOSE: To investigate the effect of dental varnish containing fluoride either with casein phosphopeptide-amorphous calcium phosphate(CPP-ACP) or bioglass on root caries. METHODS: The active roots collected in Peking University People's Hospital from April 2017 to October 2018 were randomly divided into group A, group B, group C, and group D, each with 18 teeth. All groups received toothbrushing containing fluoride, in addition, group B received another 5% sodium fluoride, group C added 5% sodium fluoride + CPP-ACP, and group D added 5% sodium fluoride + bioglass. The severity, surface roughness and mineral concentration of root caries before and after brushing were observed. SPSS 23.0 software package was used to determine the correlation between root severity and surface roughness. RESULTS: After 50 days of brushing, the hardness of group B, C and D was significantly improved (P<0.05), and was significantly better than that of group A (P<0.05). The hardness was the highest in group D after 50 days of brushing, followed by group C and B, with significant difference(P<0.05). The surface roughness scores of group B and C were significantly higher than those of group A and D after 7 days of brushing(P<0.05). The surface roughness scores of group B were significantly higher than those of group A, C and D after 14 days of brushing(P<0.05). The surface roughness scores of group D were significantly higher than those of group B and C after 50 days of brushing(P<0.05). The severity score of root caries in group A was negatively correlated with surface roughness (P<0.05). The mineral concentration of each group was increased 50 days after brushing (P<0.05). CONCLUSIONS: Application of dental varnish containing fluoride either with CPP-ACP or bioglass can effectively prevent root caries. Compared with CPP-ACP, bioglass is more stable, and can effectively improve the mineral concentration on tooth surface.


Asunto(s)
Caries Dental , Caries Radicular , Cariostáticos , Caseínas , Cerámica , Fluoruros , Humanos , Minerales , Pintura , Remineralización Dental
3.
Braz Dent J ; 31(2): 157-163, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32556015

RESUMEN

This study assessed the effectiveness of models for developing subsurface caries lesions in vitro and verified mineral changes by transverse microradiography (TMR). Enamel blocks from permanent (n=5) and deciduous teeth (n=5) were submitted to lesion induction by immersion in demineralizing solutions during 96 h, followed by pH cycles of demineralization (de) and remineralization (re) for 10 days. Two de-/re solutions were tested. Demineralizing solution "A" was composed by 2.2 mM CaCl2, 2.2 mM KH2PO4, 0.05 M acetic acid, with pH 4.4 adjusted by 1 M KOH. Demineralizing solution "B" was composed by 2.2 mM CaCl2, 2.2 mM NaH2PO4, 0.05 M acetic acid and 0.25 ppmF, with pH 4.5 adjusted by 1M KOH. Solution "A" produced cavitated lesions in permanent teeth, whereas solution "B" led to subsurface lesions in deciduous teeth. Solution "B" was then tested in enamel blocks from permanent teeth (n=5) and subsurface lesions were obtained, so that solution "B" was employed for both substrates, and the blocks were treated with slurries of a fluoride dentifrice (1450 ppm F, as NaF, n=5) or a fluoride-free dentifrice (n=5). Solution "B" produced subsurface lesions in permanent and primary teeth of an average (±SD) depth of 88.4µm (±14.3) and 89.3µm (±15.8), respectively. TMR analysis demonstrated that lesions treated with fluoride-free dentifrice had significantly greater mineral loss. This study concluded that solution "B" developed subsurface lesions after pH cycling, and that mineral changes were successfully assessed by TMR.


Asunto(s)
Caries Dental , Dentífricos , Desmineralización Dental , Cariostáticos , Esmalte Dental , Fluoruros , Humanos , Concentración de Iones de Hidrógeno , Remineralización Dental
4.
Braz Dent J ; 31(2): 164-170, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32556016

RESUMEN

The aim of this study was to compare the effects of a single application of a new calcium mesoporous silica nanoparticle (Ca2+-MSN) versus other calcium and/or fluoride products against dental erosion. Enamel blocks were half-covered and assigned to six groups (n = 10): Ca2+-MSNs; casein phosphopeptide-amorphous calcium phosphate mousse (CPP-ACP); CPP-ACP/F- (900 ppm F-); titanium tetrafluoride (TiF4 1%); sodium fluoride (NaF 1.36%); and Milli-Q® water (negative control). A single application for each product was completed on the exposed areas of the blocks and were submitted to an erosive challenge. Differences in volumetric roughness (Sa), and tooth structure loss (TSL) by use of three-dimensional noncontact optical profilometry were evaluate. Scanning electron microscopy (SEM) was performed. Analysis of variance and Tukey's test for Sa and the Kruskal-Wallis and Mann-Whitney U tests (p< 0.05) for TSL, respectively. Results: When evaluating Sa, all products presented differences in roughness when compared with the control group (p< 0.05) but not with each other (p > 0.05). However, when analyzing the TSL, it was observed that Ca2+-MSNs, TiF4, and NaF were more effective in preventing dental erosion versus CPP-ACP, CPP-ACP/F-, and Milli-Q® water (p< 0.05). In the SEM images, the negative control presented the worst loss of dental structure, with more porous enamel. Ca2+-MSNs were as effective as TiF4 and NaF to reduce the tooth structure loss.


Asunto(s)
Fluoruros , Erosión de los Dientes , Calcio , Caseínas , Esmalte Dental , Humanos , Dióxido de Silicio , Fluoruro de Sodio , Remineralización Dental
5.
Chin J Dent Res ; 23(2): 131-141, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32548604

RESUMEN

OBJECTIVE: To explore the self-assembly and gelation properties of synthetic peptides, and their efficacy on hydroxyapatite (HAP) nucleation and in situ remineralisation of initial caries lesions. METHODS: Mass spectrometry and reversed-phase high performance liquid chromatography (RPHPLC) were used to confirm the successful synthesis of peptides. Their self-assembly properties and conformation stability were evaluated using circular dichroism (CD) spectroscopy and Fourier-transform infrared spectroscopy (FTIR). Cell Counting Kit-8 (CCK-8; Dojindo, Kumamoto, Japan) was used to evaluate their cytotoxicity. The efficacy of the peptides on HAP nucleation and in situ remineralisation of initial caries lesions was explored using FTIR, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction and transverse microradiography analysis. RESULTS: Two kinds of self-assembly ß-sheet peptides named ID4 and ID8, respectively, were successfully synthesised with purities greater than 95%. Both were stable under neutral physiological conditions and had low cytotoxicity. ID4 and ID8 showed calcium responsive self-assembly properties and could self-assemble into nanofibres. Compared with ID4, ID8 resulted in the rapid formation of hydrogel with a lower concentration of calcium, and self-assembled ID8 hydrogel induced the formation of flower-like HAP and significantly promoted the remineralisation of initial enamel caries. CONCLUSION: ID8 could serve as the template to induce HAP nucleation and promote biomimetic remineralisation of initial caries lesions. These results underpin future research on peptide design, and ID8 may be a promising bioactive component for anti-caries applications.


Asunto(s)
Caries Dental , Nanofibras , Cariostáticos , Humanos , Péptidos , Conformación Proteica en Lámina beta , Remineralización Dental
6.
J Evid Based Dent Pract ; 20(1): 101416, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32381413

RESUMEN

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Effectiveness of casein phosphopeptide-amorphous calcium phosphate-containing products in the prevention and treatment of white spot lesions in orthodontic patients: A systematic review. Pithon MM, Baião FS, Sant'Anna LID, Tanaka OM, Cople-Maia L. J Invest Clin Dent 2019;e12391. SOURCE OF FUNDING: No financial support was reported. TYPE OF STUDY/DESIGN: Systematic review.


Asunto(s)
Caries Dental , Soportes Ortodóncicos , Fosfatos de Calcio , Caseínas , Fluoruros , Humanos , Fosfopéptidos , Remineralización Dental
7.
Int J Nanomedicine ; 15: 2829-2839, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32368057

RESUMEN

Objective: To investigate the remineralizing and staining effects of sodium fluoride (NaF) solution with polyethylene glycol-coated silver nanoparticles (PEG-AgNPs) on artificial dentine caries. Materials and Methods: Demineralized human dentine blocks were allocated to three groups. The blocks in group 1 underwent a topical application of a 12% silver diamine fluoride (SDF, 14,150 ppm fluoride) solution. The blocks in group 2 received a topical application of a 2.5% NaF (11,310 ppm fluoride) with PEG-AgNPs (400 ppm silver). The blocks in group 3 received deionized water. All blocks were subjected to pH cycling for 8 days. The surface morphology and cross-sectional features were investigated using scanning electron microscopy (SEM). The color parameters, crystal characteristics, lesion depth, and collagen degradation of the blocks were assessed using digital spectrophotometry, X-ray diffraction (XRD), micro-computed tomography, and spectrophotometry with a hydroxyproline assay, respectively. Results: The SEM showed that dentine collagen was exposed in group 3 but not in groups 1 and 2. The mean lesion depths in groups 1 to 3 were 118±7 µm, 121±14 µm, and 339±20 µm, respectively (groups1,2<3; p<0.001). The data indicated that fluoridated PEG-AgNPs introduced no significant color effect on dentine, but SDF caused distinct discoloration. The XRD indicated that silver chloride was formed in group 1, and fluorapatite was detected in groups 1 and 2. The concentration of hydroxyproline liberated from collagen was significantly less in groups 1 and 2 than in group 3. Conclusion: The use of NaF solution with PEG-AgNPs can remineralize artificial dentine caries and inhibit collagen degradation without causing significant tooth staining.


Asunto(s)
Caries Dental/tratamiento farmacológico , Dentina/efectos de los fármacos , Nanopartículas del Metal/uso terapéutico , Fluoruro de Sodio/farmacología , Remineralización Dental/métodos , Colágeno , Color , Dentina/metabolismo , Dentina/patología , Fluoruros/farmacología , Fluoruros Tópicos/química , Fluoruros Tópicos/farmacología , Humanos , Hidroxiprolina/metabolismo , Nanopartículas del Metal/administración & dosificación , Nanopartículas del Metal/química , Microscopía Electrónica de Rastreo , Tercer Molar/efectos de los fármacos , Tercer Molar/patología , Tercer Molar/ultraestructura , Polietilenglicoles/química , Compuestos de Amonio Cuaternario/química , Compuestos de Amonio Cuaternario/farmacología , Plata , Compuestos de Plata/química , Compuestos de Plata/farmacología , Fluoruro de Sodio/administración & dosificación , Difracción de Rayos X , Microtomografía por Rayos X
8.
J Contemp Dent Pract ; 21(3): 267-270, 2020 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-32434972

RESUMEN

AIM: To compare the efficacy of casein phosphopeptide-amorphous calcium phosphate-fluoride (CPP-ACP-F) paste and 0.2% sodium fluoride mouthwash in the prevention of dental erosion using profilometer. MATERIALS AND METHODS: The buccal surfaces of 36 premolar sound teeth were ground and polished to achieve a flat surface with silicone disks. Samples were allocated in three groups randomly. Group I was pretreated for 5 days four times a day with CPP-ACP-F paste. Group II was pretreated for 5 days with 0.2% sodium fluoride mouthwash four times a day. Group III remained as the control group without any pretreatment. In the next step, all the samples were exposed four times a day for 3 days to carbonated beverages. The samples were rinsed with saline after each erosive cycle and stored in artificial saliva. The profilometer was used to determine the surface loss. The data collected were evaluated using one-way analysis of variance (ANOVA) along with post hoc test. RESULTS: The erosion of group I (CPP-ACP-F paste) and group II (0.2% sodium fluoride mouthwash) was significantly less than that of group III (control group). The erosion in group II was significantly lower than in group I. CONCLUSION: Both sodium fluoride mouthwash and CPP-ACP-F paste are effective in the prevention of dental erosion. Sodium fluoride mouthwash shows higher reduction in dental erosion when compared with CPP-ACP-F paste. CLINICAL SIGNIFICANCE: This study contributes to the understanding of the efficacy of CPP-ACP-F paste and 0.2% sodium fluoride mouthwash in the prevention of dental erosion.


Asunto(s)
Caseínas , Fluoruros , Fosfatos de Calcio , Esmalte Dental , Antisépticos Bucales , Fosfopéptidos , Fluoruro de Sodio , Remineralización Dental
9.
Am J Dent ; 33(3): 161-164, 2020 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-32470243

RESUMEN

PURPOSE: To assess the hard tissue safety of a variety of low pH oral care rinses to dental enamel in a newly developed screening method. METHODS: Bovine enamel specimens were subjected to a cycling model that consisted of commercial mouthrinse product exposures and artificial saliva soaks based on a previously published screening method. The effect of test products on the surface of treated specimens was measured using surface microhardness (SMH). Results are presented as the change in SMH (between sound enamel baseline and cycling final). An assortment of rinse products were assessed relative to distilled water (positive control) and 1% citric acid (negative control). A priori, a product was considered safe if the change in measured SMH values over the course of six treatment cycles was both significantly greater than the negative control and was not significantly different from the positive control. A non-inferiority statistical test was applied to create a quantitative rule defining product safety. RESULTS: Products tested included two rinses with a pH in excess of 5.5, and eight with a pH less than 5.5. Four of the rinses included fluoride, while six did not. Analyses showed that all of the rinse products tested passed the non-inferiority acceptance criteria. One of the 10 marketed oral care rinses failed to meet the a priori criteria needed to be considered safe as the product was significantly better than the negative control but also significantly lower than the positive control treatment. This product had the lowest pH of all products tested and did not contain fluoride. Application of the non-inferiority statistical test showed the questionable product passing safety criteria. As a proposed method for a screening tool, further testing would be recommended based on these results. CLINICAL SIGNIFICANCE: An in vitro enamel safety screening method was applied as an assessment of the enamel demineralization safety to a number of oral care rinse products. Surface microhardness, coupled with a non-inferiority statistical evaluation, provided a reasonable approach for detecting potential product issues. Products failing this screening laboratory method may require additional testing to verify their safety on hard tissues.


Asunto(s)
Esmalte Dental , Desmineralización Dental , Animales , Cariostáticos , Bovinos , Fluoruros , Dureza , Concentración de Iones de Hidrógeno , Antisépticos Bucales , Remineralización Dental
10.
Indian J Dent Res ; 31(2): 217-223, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32436900

RESUMEN

Background: The early enamel lesions are reversible as it is a process involving mineral transactions between the teeth and saliva. Aim: To evaluate the efficiency of three different tooth pastes on remineralization potential of initial enamel lesions using Vickers Micro hardness Test and Scanning electron microscopy. Materials and Methods: Artificial carious lesions were prepared in human enamel with demineralizing solution. The treatment agents included were Colgate sensitive plus® toothpaste, Regenerate enamel science™ toothpaste, BioRepair® toothpaste and control as Deionized water. All the samples were subjected to treatment solutions as per the pH cycling model for 12 days to simulate the daily oral environment's acid challenge. The remineralization parameters-surface hardness and surface roughness of enamel blocks were evaluated with Vickers indenter and Scanning electron microscope respectively. Statistical Analysis: ANOVA test was used to check mean differences between the groups. Post hoc analysis was done using Tukey's post hoc test. SEM images were graded according to Bonetti et al grading criteria. Results: As per statistical analysis, maximum remineralization of enamel blocks occurred after applying Colgate Sensitive Plus® tooth paste followed by BioRepair® tooth paste and Regenerate enamel Science™ toothpaste. Least remineralization potential was shown by control group. Conclusion: Colgate sensitive plus tooth paste with Pro Argin™ formula can be regarded as a potential remineralising agent. It can be concluded as a noninvasive means of managing early enamel carious lesions.


Asunto(s)
Remineralización Dental , Pastas de Dientes , Cariostáticos , Esmalte Dental , Electrones , Fluoruros , Dureza , Humanos , Microscopía Electrónica de Rastreo
11.
Arch Oral Biol ; 112: 104684, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32120052

RESUMEN

OBJECTIVES: The aim of this study was to evaluate the efficiency of xylitol chewing gums enriched with propolis, remineralizing softly demineralized dentin in vitro. DESIGN: Four groups of chewing gum were developed; Group1: xylitol (1.8 %), Group2: xylitol + casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) (3%), Group3: xylitol+Hydroxyapatite (3%) and Group4: xylitol + propolis (5%). A control group was designed without chewing gum, but with artificial saliva. Sections of embedded crowns and cleaned roots of twenty five bovine incisors were demineralized in carbonated drink. Crown specimens were half-varnished. Remineralization process was run for all the dental specimens in the 4 groups with gum extracts and in the control group with artificial saliva for 20 min at 37 °C three times a day during 7 days. Mineral contents were evaluated by scanning electron microscopy with energy dispersive X-ray spectroscopy (EDX-SEM). Surface morphology and roughness were analyzed using atomic force microscopy (AFM). Micro-hardness was measured using Vickers micro-hardness tester among varnished and unvarnished sides. RESULTS: Calcium/Phosphate mean ratio showed a significant decrease between the control group, group1, group2 and group4. Control group and group3 were not significantly different. Micro-hardness increased significantly for all treated groups. AFM showed obstruction of dentinal tubules in all the groups and roughness decreased in the treated side of the dentin compared to the untreated side for tested groups. CONCLUSION: Xylitol chewing gum enriched with propolis showed dentinal tubules occlusion, significant improvement of micro-hardness and slight decrease in roughness. Ca/P ratio analysis suggests that a mineral compound other than hydroxyapatite is responsible of tubules occlusion.


Asunto(s)
Goma de Mascar , Dentina , Própolis/farmacología , Remineralización Dental , Xilitol/farmacología , Animales , Bovinos
12.
Braz Dent J ; 31(1): 44-51, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32159705

RESUMEN

This study evaluated the impact of using biomimetic analogs (poly-acrylic acid and sodium tri-meta-phosphate) on dentin remineralization using two cement materials, the first is calcium silicate based and the second is calcium hydroxide based materials. Two standardized occlusal cavities (mesial and distal) were prepared within dentin after removal of occlusal enamel. Artificial demineralized dentin was induced through pH cycling (8 h in demineralizing and 16 h in remineralizing solutions). Demineralized cavities were divided into four groups; two groups received cement materials. The other groups were first treated with biomimetic analogs then restored with pulp cement materials. Teeth were sectioned buccolingually into two halves. Treated cavities with analogs were stored in simulated body fluid containing poly-acrylic acid. Untreated cavities were stored in simulated body fluid only. Ground unstained sections of demineralized dentin were examined using light microscope. Specimens were examined after 1, 6 and 12 weeks of storage using energy dispersive X-ray Spectroscopy (EDX) and Vickers microhardness was evaluated. Two-way ANOVA was used to analyze data statistically. Calcium silicate-based cement group with biomimetic analogs showed the highest statistically significant calcium and phosphorous wt% in addition to highest surface hardness values after 12 weeks of storage. Demineralized dentin ground sections showed increase in light zones after total period of storage. Calcium silicate-based cement showed the best ability to enrich the artificial carious dentin with ions for remineralization. Using biomimetic analogs had a significant impact on demineralized dentin surface hardness improvement.


Asunto(s)
Biomimética , Remineralización Dental , Cementos Dentales , Dentina , Silicatos
13.
Dent Mater ; 36(4): 501-511, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32035669

RESUMEN

OBJECTIVE: Dentin remineralization is of considerable clinical interest for dentin hypersensitivity and developing biomimetic analogs that can regulate hydroxyapatite (HAp) nucleation and growth remains a challenge. This study aimed to evaluate in vitro the potential for dentin remineralization using the following biomimetic in situ prepared poly(catechols): poly(dopamine), poly(DOPA), poly(caffeic acid) and a synthesized DOPA-peptide possessing collagen and calcium-binding domains (DOPA-Ahx-(Gly)3-(Glu)5). METHODS: Dentin samples were immersed in a freshly prepared phosphate-buffered saline (PBS) containing the respective catechol and laccase. After the reaction, they were immersed in calcium and phosphate remineralization solution, which was changed every day for 10 days. Samples of intact and demineralized dentin were used as control groups and kept in deionized water under the same experimental conditions. The remineralized dentin was characterized by scanning electron microscopy (SEM), Micro-energy dispersion X-ray fluorescence spectroscopy (µEDX) and X-ray diffraction (XRD). RESULTS: The application of different poly(catechols) and DOPA-peptide promoted crystal nucleation and the formation of HAp, which partially covered both the dentin surface and dentinal tubules walls. SIGNIFICANCE: By mimicking the role of charged non-collagenous proteins in vivo, polymers consisting of catechol groups showed the ability to modify demineralized dentin surface properties, promoting mineral formation. The use of poly(catechols) may be encouraged for the development of a therapeutic technique for dentin hypersensitivity.


Asunto(s)
Sensibilidad de la Dentina , Remineralización Dental , Catecoles , Dentina , Humanos , Microscopía Electrónica de Rastreo
14.
Am J Dent ; 33(1): 12-16, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32056409

RESUMEN

PURPOSE: To evaluate the in vitro effects of commercially available charcoal dentifrices on Streptococcus mutans biofilm development and their ability to prevent enamel demineralization. METHODS: Streptococcus mutans biofilm was formed on polished bovine enamel specimens (n= 9 per treatment), and treated twice-daily for 120 seconds over the course of 5 days with: charcoal dentifrice containing fluoride (1,000 ppm F) (CF+), fluoride-free charcoal dentifrice (CF-), regular fluoride (1,100 ppm F) dentifrice (F+ ), or regular fluoride-free dentifrice (F-). Chlorhexidine (CHX, 0.12%) and deionized water (DIW) were used as positive and negative controls, respectively. Biofilms were analyzed for bacterial viability (colony-forming units, CFU). The pH of the medium was measured daily. Enamel specimens were analyzed using Vickers microhardness ( HV) and transversal microradiography (TMR). Data were analyzed using one-way ANOVA followed by post-hoc tests (α= 0.05). RESULTS: F+ showed higher pH values than CF+ and CF-, and CF- presented higher pH than CF+, showing that CF+ did not have inhibitory effects on the acidogenicity of cariogenic biofilms. CFU was significantly decreased when specimens were treated with CF+, CF- and F+, compared to specimens treated with DIW (P≤ 0.035) or F- (P≤ 0.001), respectively. However, the reduction observed was minimal (approximately 1 log). CF+ and CF- were less effective than F+ in preventing enamel demineralization as determined using HV (P= 0.041 and P= 0.003, respectively) and TMR ( P≤ 0.001). Both charcoal dentifrices (CF+, CF-) did not show relevant inhibition of S. mutans biofilm growth. Additionally, neither product prevented enamel demineralization compared to a regular fluoride-containing dentifrice. CLINICAL SIGNIFICANCE: The tested charcoal dentifrices did not exhibit anticaries potential.


Asunto(s)
Dentífricos , Desmineralización Dental , Animales , Biopelículas , Cariostáticos , Bovinos , Carbón Orgánico , Esmalte Dental , Fluoruros , Fluoruro de Sodio , Streptococcus mutans , Remineralización Dental
15.
Am J Dent ; 33(1): 43-47, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32056415

RESUMEN

PURPOSE: To investigate the effect of acid etchants with different low concentrations on remineralization of white spot lesion (WSL). METHODS: WSL were prepared on buccal surfaces of 100 intact premolars using the methyl cellulose gel/lactic acid method. The samples were then placed in a remineralizing solution in addition to fluoride application twice daily for 5 minutes. The changes were quantified weekly using the Quantitative Light-induced Fluorescence (QLF) system. When changes in fluorescence radiance approached zero, each sample was etched with one of the following acids; 5% phosphoric acid, 10% phosphoric acid, 5% polyacrylic acid or 10% polyacrylic acid for 15 seconds, washed, dried, and placed again in the remineralizing solution. Two samples were randomly selected from each group for transverse microradiography (TMR) and scanning electron microscopy (SEM) analysis. RESULTS: The 10% polyacrylic acid group showed the most significant improvement in fluorescence gain over the second phase of remineralization. It also showed partial loss of surface minerals without affecting enamel thickness as the phosphoric acid did. Additionally, 10% polyacrylic acid created the largest number of pores and smallest in size when compared to phosphoric acid, thus enhancing remineralization more efficiently than phosphoric acid without compromising the enamel outermost layer. CLINICAL SIGNIFICANCE: The findings of this study may improve the remineralization of WSL from the bottom of the lesion instead of precipitation on the outermost layer of the lesion leaving a better quality of enamel. 10% polyacrylic acid enhanced remineralization more efficiently than phosphoric acid without compromising the enamel outermost layer.


Asunto(s)
Caries Dental , Remineralización Dental , Esmalte Dental , Fluoruros , Humanos , Microrradiografía
16.
Dent Mater ; 36(3): 456-467, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32008748

RESUMEN

OBJECTIVE: The aim is to investigate the potential significance of combining minimally invasive high-intensity focused ultrasound (HIFU) with hydroxyapatite (HA) nanorods treatment for the remineralization of demineralized coronal dentine-matrix. METHODS: HA having nanorods structure were synthetized using ultrasonication with precipitation method. HA nanorods were characterized by TEM for average-size/shape. Following phosphoric acid demineralization, dentine specimens were treated with HA-nanorods with/without subsequent HIFU exposure for 5 s, 10 s and 20 s then stored in artificial saliva for 1-month. Dentine specimens were characterized using different SEM and Raman spectroscopic techniques. In addition, the biochemical stability and HA-nanorods were examined using ATR-FTIR to observe attachment of nanoparticles. Also, surface nanoindentation properties were evaluated using AFM in tapping-mode. RESULTS: HA-nanorods displayed well-defined, homogenous plate-like nanostructure. TEM revealed intact collagen-fibrils network structure with high density due to obliteration of interfibrillar spaces with clear evidence of remineralization in combined HA/HIFU treatment. With HA-nanorods treatment collagen-network structure was visible, consisting of fibrils interlaced into a compact pattern with evidence of minerals deposition. AFM investigation revealed clear mineral formation with the increase of HIFU exposure time. Bands associated with inorganic phase dominate well in HIFU exposed specimens with PO stretching within dentine mineral identified at 960 cm-1. Characteristic dentine structure for control and HIFU 20 s specimens is reflected as oscillatory mean Amide-I intensity with measurement giving a precise sinusoidal response of polarization angle ß within dentinal tissue. Nanoindentation testing showed a gradual significant increase in elastic-modulus with the increase in HIFU exposure time after 1-month storage. FTIR spectrum of the HIFU exposed dentine displayed bands at 1650 cm-1, 1580 cm-1 and 1510 cm-1 that can be attributed to Amide-I, II and III. SIGNIFICANCE: The synergetic effect of HIFU exposure on remineralization potential of demineralized dentine-matrix following nano-hydroxyapatite treatment was revealed. This synergetic effect is dependent on HIFU exposure time.


Asunto(s)
Durapatita , Nanotubos , Dentina , Saliva Artificial , Remineralización Dental
17.
Arch Oral Biol ; 111: 104638, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31901573

RESUMEN

OBJECTIVE: The purpose of this study was to investigate the effects of Lemon essential oil (LEO) and Limonene (LIM) in the progress of early caries. DESIGN: LEO and LIM were selected as experimental medicine, while sodium fluoride (NaF) and deionized water (DW) were positive and blank controls, respectively. Bovine incisors were used to establish enamel and dentin early caries models by demineralization method in vitro. Then specimens were subjected to pH cycling. Calcium and phosphate release of demineralizing solution were measured by an automatic biochemical analyzer; Surface microhardness tester and energy dispersive X-ray spectrometer were used to detect the surface microhardness recovery and calcium- phosphate ratio on tooth surface; Degraded collagen matrix by collagenase was investigated by assaying hydroxyproline. RESULTS: Calcium release of dentin demineralizing solution of LEO group was lower than DW group's and higher than NaF group's. Both of LEO and LIM groups, the surface microhardness recovery were significantly lower than those of NaF group, which were similar to DW group. Dentin surface calcium- phosphate ratio of LEO and LIM groups were lower than those of NaF group and higher than those of DW group. Hydroxyproline concentration in the remineralizing solution of LEO and LIM groups were lower than DW groups' and higher than NaF groups'. CONCLUSIONS: LEO and LIM have influence on the progress of dentin early caries, which can stabilize its structure by inhibiting collagen degradation. Meanwhile, these medicines may provide a new drug choice for the prevention and treatment of early root caries.


Asunto(s)
Cariostáticos/farmacología , Caries Dental , Esmalte Dental/efectos de los fármacos , Limoneno/farmacología , Aceites Volátiles/farmacología , Aceites Vegetales/farmacología , Animales , Bovinos , Concentración de Iones de Hidrógeno , Fluoruro de Sodio/farmacología , Desmineralización Dental , Remineralización Dental
18.
Eur Arch Paediatr Dent ; 21(1): 85-93, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31134470

RESUMEN

PURPOSE: To compare the effects of a fluoride dentifrice alone, versus a topical CPP-ACP crème used as a dentifrice, versus the combination of both agents (fluoride dentifrice followed by CPP-ACP crème) on early caries lesions (ECL) in primary anterior teeth of children over a 6-month period. METHODS: In this single blinded randomised clinical trial, children (n = 114, aged 4-5 years) with high caries risk and who had at least one ECL (ICDAS 2) on their primary anterior teeth were allocated to three groups. Subjects used different agents twice daily: fluoride dentifrice (500 ppm F, n = 42), CPP-ACP crème (10% w/v, n = 35), and the fluoride dentifrice followed by CPP-ACP crème (n = 37). Changes in lesions were tracked using QLF, comparing the baseline situation to 3 and 6 months of treatment. RESULTS: QLF changes consistent with mineral gain (∆F/fluorescence, lesion area) occurred in all three groups (baseline versus 3 months or 6 months, p ≤ 0.05 for all three groups). Differences between treatment groups were not statistically significant (p > 0.05). CONCLUSION: The topical CPP-ACP crème used alone gave effects similar to the fluoride dentifrice; however, the combination of the two did not give additive benefits over either agent used in isolation.


Asunto(s)
Caries Dental , Dentífricos , Cariostáticos , Caseínas , Niño , Preescolar , Fluoruros , Humanos , Minerales , Fluorescencia Cuantitativa Inducida por la Luz , Remineralización Dental
19.
Dent Mater J ; 39(1): 62-68, 2020 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-31723068

RESUMEN

Protecting resin-dentin interfaces from hydrolytic and enzymatic degradation is critical for the longevity of adhesive restorations. In recent years, several strategies have been tested in vitro to induce apatite precipitation within interfibrillar and intrafibrillar collagen spaces, as well as in resin-sparse regions where the adhesive infiltration was incomplete. Also, the presence of calcium ions and other metallic ions has shown an inhibitory effect on enzymatic activity. Ion-releasing particles and biomimetic analogs have been studied for hybrid layer remineralization. Overall, remineralization strategy is dependent on the remaining mineral content. In partially demineralized dentin, residual apatite crystallites serve as nucleation sites for calcium and phosphate ions precipitation and crystal growth ("top-down" remineralization). In completely demineralized dentin where crystallites are absent (e.g., acid etched dentin) the use of mineral nano-precursors assisted by non-collagenous proteins analogs are necessary ("bottom-up" remineralization). This article reviews the approaches for hybrid layer remineralization and resin-dentin interface preservation.


Asunto(s)
Biomimética , Recubrimientos Dentinarios , Cementos Dentales , Dentina , Microscopía Electrónica de Transmisión , Remineralización Dental
20.
Dent Mater J ; 39(1): 118-125, 2020 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-31582598

RESUMEN

To explore the effects of infiltration resin, casein phosphate polypeptide-amorphous calcium phosphate (CPP-ACP) and fluoride on microhardness and micromorphology of irradiated enamel. Sixty human permanent teeth were mesiodistally sectioned, yielding 120 enamel samples, which randomly divided into 8 groups: G1: blank control; G2: irradiation control; G3: irradiation+fluoride; G4: irradiation+CPP-ACP; G5: irradiation+CPP-ACP+fluoride; G6: irradiation+infiltration resin; G7: irradiation+infiltration resin+fluoride; G8: irradiation+infiltration resin+CPP-ACP. A progressive improvement was observed on the superficial morphology of enamel treated with different anti-caries procedures. The order of microhardness values after irradiation from the highest to the lowest was as follows: G1>G8~G7>G6>G5>G4~G3>G2. CPP-ACP, infiltration resin and fluoride can effectively restore the direct destruction of enamel caused by irradiation and promote the occurrence of remineralization. Infiltration resin and its combined effects with fluoride or CPP-ACP have the most potential anti-caries agent to resist radiation-caries.


Asunto(s)
Caries Dental , Traumatismos de los Dientes , Cariostáticos , Caseínas , Esmalte Dental , Fluoruros , Humanos , Fosfatos , Remineralización Dental
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