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1.
Food Chem ; 398: 133911, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-35969989

RESUMEN

A superior sectioning sample preparation protocol is the basic guarantee for maintaining data reliability in MALDI-TOF-MSI analysis. Despite significant advances in sample preparation, visualization of lipids in hard and dry cereals remains difficult due to their inherent physicochemical properties. Thus, a cryosections preparation method was designed via poly-l-lysine soaking, conductive tape adhesion and embedding medium fixation, which preserved the spatial integrity of lipids in cereals without causing analyte delocalization and obvious background interference. Higher signal intensity and clearer imaging of lipids in rice, wheat and barley (Qingke) were obtained using the established sample preparation method. Moreover, the spatial distribution of lipids was visualized in rice with different storage years, which found that the phosphatidylinositols (PIs) and phosphatidylglycerols (PGs) could be used for evaluation of rice aging degree. Our study provided molecular level guidance for further discussion of rice aging mechanism, rice quality evaluation and safety monitoring.


Asunto(s)
Grano Comestible , Lípidos , Reproducibilidad de los Resultados , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos
2.
Food Chem ; 398: 133883, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-35969995

RESUMEN

Food safety is regarded as a crucial factor in both human health and economic progress. This study focuses on the fabrication of a label-free surface-enhanced Raman scattering (SERS) sensor for rapid sensing of three main mycotoxins (aflatoxin B1 (AFB1), ochratoxin A (OTA), ochratoxin B (OTB)) in rice using the optimized rough silver nanoparticles (AgNPs@K30) with enhancement factor (EF) 1.58 × 107 coupled multivariate calibration. Two variable selection chemometric calibration methods were comparatively applied. And genetic algorithm-partial least square achieved optimum correlation coefficient = 0.9797, 0.9779, and 0.9827, respectively for AFB1 ranging from 0.5 to 250 µg/Kg, for OTA and OTB 1 to 500 µg/Kg. The limit of detection (LOD) = 1.145, 1.133, and 1.180 µg/Kg, respectively, were determined according to principal component analysis-calibrated loading weight approach. And the great stability and reproducibility revealed the prepared SERS sensor has the potential to predict AFB1, OTA, and OTB in real rice samples.


Asunto(s)
Nanopartículas del Metal , Micotoxinas , Oryza , Aflatoxina B1/análisis , Calibración , Humanos , Límite de Detección , Micotoxinas/análisis , Reproducibilidad de los Resultados , Plata , Espectrometría Raman/métodos
3.
J Hazard Mater ; 443(Pt B): 130316, 2023 02 05.
Artículo en Inglés | MEDLINE | ID: mdl-36370477

RESUMEN

Realizing efficient detection of ultra-low concentrations of hazardous gases contributes to air pollution monitoring, ecosystem and human health protection. Herein, we firstly fabricated the nanoflower-like WO3/WS2 composites by a facile process to highly sensitively detect NO2 at room temperature. The WO3 content in the WO3/WS2 composites can be adjusted by altering the calcination temperature, and the WO3 nanoparticles disperse uniformly on the WS2 surface, forming the WO3/WS2 heterojunction. The room-temperature responses of WO3/WS2 composites gradually climb with the NO2 concentration increasing from 0.005 to 5 ppm, and the WW-280 and WW-300 composites possess the optimal gas sensitivity when the NO2 concentrations are lower and higher than 100 ppb, respectively. In particular, the two WO3/WS2 composites present the limitation of detection (LOD) of ≤ 5 ppb, and they exhibit the excellent selectivity, good reproducibility and long-term stability towards NO2. A possible gas sensing mechanism was also proposed from the point of views of gas adsorption, redox reactions and electron transfer. The appropriate WO3 content and molar ratio of hexagonal to monoclinic WO3, and the formation of WO3/WS2 p-n heterojunction can contribute to the high sensitivity of WO3/WS2 composite to various concentrations of NO2. This work offers a promising gas sensing material for room-temperature detection to low concentrations of NO2.


Asunto(s)
Ecosistema , Dióxido de Nitrógeno , Humanos , Temperatura , Reproducibilidad de los Resultados , Gases
4.
J Hazard Mater ; 443(Pt B): 130234, 2023 02 05.
Artículo en Inglés | MEDLINE | ID: mdl-36372024

RESUMEN

Rapid, sensitive and specific detection of bacteria is of great importance. Herein, we developed a versatile biosensing platform for ultrasensitive detection of pathogenic bacteria, termed as SCENT-Cas (Silver nanoCluster Empowered Nucleic acids Test using CRISPR/Cas12a). Simply, the species-specific invA gene of Salmonella typhimurium (S. typhi) was isothermally amplified using LAMP, which subsequently triggered the trans-cleavage of CRISPR/Cas12a. The trans-cleavage degraded any single-stranded DNA (ssDNA) non-specifically. A DNA-templated AgNCs probe was then employed, in which green fluorescence emissive AgNCs effectively converted to red fluorescence emissive AgNCs when placed in close vicinity to a pre-designed converter ssDNA. As such, the trans-cleavage was utilized for shredding converter ssDNA, enabling the green-to-red fluorescent change to form a ratiometric biosensing platform. With this strategy, target nucleic acid was dexterously converted into ratiometric fluorescence that was recorded to detect as low as 1 CFU/mL S. typhi with a dynamic range from 1 to 108 CFU/mL. To our knowledge, this is the first report regarding the use of ratiometric fluorescence in CRISPR/Cas-based detection, which minimizes interference and improves reliability. Lastly, this proposed strategy was challenged by detecting S. typhi contamination in real food samples. Our work enriches CRISPR/Cas toolbox in biosensing by providing a desirable method for bacterial detection.


Asunto(s)
Técnicas Biosensibles , Plata , Salmonella typhimurium/genética , Sistemas CRISPR-Cas , Reproducibilidad de los Resultados , Técnicas Biosensibles/métodos
5.
J Pharm Biomed Anal ; 223: 115157, 2023 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-36379101

RESUMEN

Qi-Lin pill (QLP) is an effective traditional Chinese medicine prescription (TCMP) that has been used for the treatment of the oligoasthenozoospermia in China. Recently, some articles described the pharmacological effects of QLP and multiple ingredients in QLP contribute to its effects. However, the pharmacokinetic and target tissue distribution data of QLP are still unknown. In the present study, according to the Bioanalytical Method Validation Guidance of FDA, a sensitive and selective UPLC-MS/MS method was developed and validated for simultaneous determination of multiple constituents in rat plasma and testicular tissue, including morusimic acid A, codonopyrridium B, magnoflorine, emodin, 2,3,5,4'-tetrahydroxystilbene-2-O-ß-D-glucoside (THSG), ecliptasaponin A, paeoniflorin, albiflorin, gallic acid, danshensu, salvianolic acid A, catechin, isosinensetin, nobiletin, formononetin, calycosin, icariside II, icariin and epimedin C. For 19 analytes, the LLOQs reached 0.01-4 ng/mL. And all calibration curves showed favorable linearity (r ≥ 0.9903) in linear ranges. The intra-day and inter-day precision (relative standard deviation) for all analytes was less than 14.92 %, and the accuracies (as relative error) were in the range of - 6.44 % to 6.22 %. Extraction recoveries and matrix effects of analytes and IS were acceptable. All analytes were stable during the assay and storage in plasma samples. The method was successfully applied for the pharmacokinetics and testis distribution of multiple chemical constituents in QLP after a single oral dose. As a result, high exposure of danshensu, gallic acid, paeoniflorin and albiflorin were observed in rat plasma and testicular tissue. Among the flavonoids, isosinensetin and nobiletin had high exposure in testicular tissue. Moreover, alleviation of progesterone reduction was evaluated in H2O2-induced R2C leydig cells, and danshensu, gallic acid, paeoniflorin, albiflorin and nobiletin showed potent activity. Therefore, these five components were considered to be the effective components of QLP due to their relatively high exposure in vivo and biological activity. This finding also provided relevant information on action mechanism of QLP in the treatment of oligoasthenozoospermia.


Asunto(s)
Medicamentos Herbarios Chinos , Espectrometría de Masas en Tándem , Animales , Masculino , Ratas , Cromatografía Líquida de Alta Presión/métodos , Cromatografía Liquida , Medicamentos Herbarios Chinos/farmacocinética , Ácido Gálico , Peróxido de Hidrógeno , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem/métodos , Testículo , Distribución Tisular
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 287(Pt 1): 122073, 2023 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-36399817

RESUMEN

By coupling thin-film microextraction (TFME) with surface enhanced Raman scattering (SERS), a facile method was developed for the determination of thiram in the complex matrix (orange juice or grape peel). The substrate of TFME was made by self-assembling silver sol on the silicon wafer to form a three-dimensional (3D) silver nanonetwork structure, without adding any template, which was used for TFME and SERS detection, respectively. The substrate exhibits high reproducibility with a relative standard deviation of about 7.32 % in spot and spot SERS intensity. The SERS signal intensity at a shift of 1384 cm-1 and the thiram concentration showed good linearity in the range of 0.01-5 µg/L and the linear correlation coefficient was 0.9912. The detection limit for thiram was found to be 0.01 µg/L. The TFME-SERS method was applied for the determination of thiram in fruit juice and the results were obtained very well. Therefore, this method is expected to play a role in the detection of trace pollutants.


Asunto(s)
Espectrometría Raman , Tiram , Plata , Reproducibilidad de los Resultados , Jugos de Frutas y Vegetales
7.
J Clin Anesth ; 84: 111008, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36399854

RESUMEN

STUDY OBJECTIVE: Establish the transcultural validity of Anesthetists Non-Technical Skills (ANTS) in a Spanish-speaking country. DESIGN: Prospective cohort. SETTING: Clinical simulation center. SUBJECTS: Forty-two Anesthesia PY2 and PY3 residents participated in the study. INTERVENTIONS: Four clinical scenarios simulating anesthesia crises were assessed with a Spanish version of ANTS. Every simulated scenario was run twice with a time span of 3 to 4 months between them. MEASUREMENTS: Two anesthesiologists independently assessed all simulated sessions using ANTS. The ANTS indicators of construct validity were obtained by confirmatory factor analysis. Various goodness-of-fit indices of the factorial model were calculated: Comparative Fit Index (CFI); Tucker-Lewis Adjustment Index (TLI) and Root Mean Square Error of Approximation (RMSEA). The standardized factor loadings and the determination coefficient (R2) was also estimated. MAIN RESULTS: A total of 212 clinical scenarios were analyzed. The specified factorial model had the same grouping of elements in four domains as the original version of ANTS. The CFI index and the TLI were 0.99 and the RMSEA reached 0.07 (95% CI 0.06-0.08). All the standardized factor loadings were found to be >0.4. Also, the elements obtained an R2 value that fluctuated between 0.54 and 0.92. CONCLUSIONS: The Spanish version of ANTS is a valid, reliable and a useful tool to assess non-technical skills in Spanish-speaking countries. The applicability of the instrument was comparable to the original setting. The high reliability of ANTS in our setting allows us to propose its use not just in an educational and research setting; it can be used as an assessment tool of non-technical skills.


Asunto(s)
Anestesistas , Comparación Transcultural , Humanos , Estudios Prospectivos , Reproducibilidad de los Resultados , Anestesiólogos
8.
J Hazard Mater ; 443(Pt B): 130357, 2023 02 05.
Artículo en Inglés | MEDLINE | ID: mdl-36444062

RESUMEN

Accurate estimates of spatiotemporally resolved Polychlorinated dibenzo-p-dioxins (PCDD/Fs, or dioxins) emissions are critical for understanding their environmental fate and associated health risks. In this study, by utilizing an empirical regression model for PCDD/Fs emissions, we developed a global emission inventory for 17 toxic PCDD/Fs congeners from 8 source sectors with a spatial resolution of 1° × 1° from 2002 to 2018. The results show that PCDD/Fs emissions decreased by 25.7 % (12.5 kg TEQ) between 2002 and 2018, mostly occurring in upper- and lower-middle income countries. Globally, open-burning processes, waste incineration, ferrous and nonferrous metal production sectors and heat and power generation were the major source sectors of PCDD/Fs. Spatially, high PCDD/Fs emissions were mainly identified in East and South Asia, Southeast Asia, and part of Sub-Saharan Africa. We find that the declining trend of dioxin emissions over the past decades terminated from the early 2010s due to increasing significance of wildfire induced emissions in the total emission. The PCDD/Fs emission inventory developed in the present study was verified by inputting the inventory as initial conditions into an atmospheric transport model, the Canadian Model for Environmental Transport of Organochlorine Pesticides (CanMETOP), to simulate PCDD/Fs concentrations in air and soil. The predicted concentrations were compared to field sampling data. The good agreement between the modeled and measured concentrations demonstrates the reliability of the inventory.


Asunto(s)
Dioxinas , Dibenzodioxinas Policloradas , Dibenzofuranos , Reproducibilidad de los Resultados , Canadá
9.
Biosens Bioelectron ; 220: 114906, 2023 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-36402099

RESUMEN

Nanobody(Nb) is too small to carry more signal reporters, which often leads to low sensitivity in immunoassay. Herein, we proposed a novel immunoprobe integration of Nb and biomimetic mineralized metal-organic frameworks(MOF), in which plenty of succinylated horseradish peroxidase(sHRP) were encapsulated within a single MOF and the Nb was assembled on the biomimetic mineralized MOF. It overcomes the dilemma that the Nb is difficult to carry more signal reporters. Meanwhile, the mineralized MOF can protect the sHRP from denaturation and facilitate the transport of substrates to the active sites of sHRP. Electrosensing of aflatoxin B1(AFB1) was realized with a competitive format in which the target AFB1 and immobilized artificial antigen were competing for binding with the immunoprobe. Additionally, the detection signal was enlarged by the catalysis of this immunoprobe to 4-chloro-1-naphthol for producing precipitations, which blocked the channels of the immunoprobe and the redox probes of Fe(CN)63-/4- was difficult to reach the electrode surface through the channels. Hence, the as-prepared immunosensor exhibited good voltammetry responses towards the determined AFB1 in a linear range of 50.0 fg/mL - 20.0 ng/mL with a detection limit of 20.0 fg/mL. The specificity, stability, and reproducibility of this immunosensor were satisfactory. This work may provide an alternative idea for the application of Nb in immunoassay, and the idea may also be applicable to other bio-recognition elements for immunoassay.


Asunto(s)
Aflatoxina B1 , Técnicas Biosensibles , Biomimética , Inmunoensayo , Reproducibilidad de los Resultados
10.
Biosens Bioelectron ; 220: 114915, 2023 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-36403491

RESUMEN

The high worldwide mortality and disproportionate impact of cardiovascular diseases have emerged as the most significant global health burden, unfortunately, unmet by the traditional detection methods. Therefore, developing a rapid, sensitive, selective, and rugged biosensor for the precise classification/quantification of cardiac biomarkers is a stepping stone for the future generation of cardiac healthcare. We demonstrate a facile, time-efficient, and scalable biosensor for classifying the FDI approved gold standard cardiac biomarker Troponin-I (cTnI) in untreated human serum matrix, built-on 2-D SnS2 and 1-D MWCNT composite transducer and decision-tree based explainable machine learning (ML) algorithm. The proposed methodology is further enhanced using an inimitable Operating-Voltage-Selection-Algorithm (OVSA), which boosts ML accuracy to ∼100%. The near-perfect classification is realized by strategically incorporating this two-step algorithm-first the OVSA, then the heuristic and ML approaches on the selected dataset. Dynamic concentrations of the biomarker (100 fg/mL to 100 ng/mL) are estimated with high sensitivity, ∼71 (ΔR/R) (ng/mL)-1cm-2 and low limit of detection (0.02 fg/mL), aiding to the prediction and prognosis of acute myocardial infarction. The hyperparameter tuning and feature engineering improve the decision process of the ML algorithm, fostering robustness against data variability. Feature importance indices, namely the Gini index, Permutation Importance, and SHAP values, portray 'Voltage' as the most important feature, further justifying our insight into the OVSA. The biosensor's specificity, selectivity, reproducibility and stability are effectively demonstrated with the sampling to result reporting time of just 20 min, establishing it as a potential candidate for clinical testing.


Asunto(s)
Técnicas Biosensibles , Infarto del Miocardio , Humanos , Troponina I , Reproducibilidad de los Resultados , Aprendizaje Automático , Algoritmos , Biomarcadores
11.
Biosens Bioelectron ; 220: 114888, 2023 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-36410156

RESUMEN

In order to address the need for long-term, in-situ and inexpensive monitoring of dissolved oxygen (DO), a chitin-carrying microbial fuel cell (MFC) based DO sensor was developed using sediment anolyte, which had an extremely low cost of US$12.17 and comparable performance to certain commercial sensors. The MFC based DO sensor had a long lifetime of over half a year with chitin as the fuel, attributed to the syntrophic interactions between fermentative and exoelectrogenic microbes that were well developed for chitin degradation in anaerobic condition with sediment filling in the anode chamber. The use of sediment anolyte introduced hindered diffusion in the porous media, enabling the use of glass fiber as the separator to replace the ion exchange membrane and thus resulting in a much lower cost. Field tests of this MFC based DO sensor were conducted in fresh and saline waters respectively. Excellent performance was achieved with average deviations of <4.5% to three commercial methods of fiber optic sensor (HQ40d, HACH company, USA), Clark type sensor (Pro20i, YSI company, USA) and iodometry. This low-cost MFC sensor also showed a high reliability, with the same response of current generation to different DO levels in random 17-times tests, indicating its great market potentials for in-situ DO monitoring.


Asunto(s)
Fuentes de Energía Bioeléctrica , Técnicas Biosensibles , Oxígeno , Reproducibilidad de los Resultados , Quitina
12.
Biosens Bioelectron ; 220: 114929, 2023 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-36417777

RESUMEN

Molecularly imprinted polymers (MIPs) are widely used as artificial recognition element in sensing field, but their electrochemical sensing performances are generally affected by their poor catalytic activity and unruly condition change. In this work, an MIP film with catalysis (Fe-DMMIP) is constructed by electrodeposition of Fe-coordinated aminophenanthroline and 3,4-ethylenedioxythiophene on N, S doped C material, using cannabinoid (CBD) as template molecule. Due to the presence of Fe-N active sites, the obtained Fe-DMMIP possesses enzyme-like catalytic activity besides conventional recognition capability. Accordingly, the sensor exhibits high electrocatalytic activity and selectivity. Moreover, the Fe-DMMIP can produce a stable and well-defined signal as an internal reference around 0 V (vs. Ag/AgCl) for ratiometric sensing. Under the optimal conditions, the ratiometric signal is linear to CBD concentration in the range of 0.004-0.8 µmol L-1 (R2 = 0.9946) with a detection limit of 2.9 nmol L-1. The ratiometric sensor shows high reproducibility, stability and applicability. In addition, through replacing the template molecule, the resulting biomimetic sensor also exhibits good performance in sensing other psychoactive substances such as melatonin and 5-hydroxytryptophan, with LODs of 19 nmol L-1 and 8 nmol L-1for them, respectively. Therefore, the developed sensing platform has good prospects, and this work provides a new way for developing ratiometric electrochemical sensors with high sensitivity, reproducibility and anti-interference ability.


Asunto(s)
Técnicas Biosensibles , Polímeros Impresos Molecularmente , Reproducibilidad de los Resultados , Biomimética , Catálisis
13.
Addict Behav ; 137: 107521, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36332516

RESUMEN

Few studies have examined Alcohol Use Disorders Identification Test (AUDIT) measurement invariance and no study has done so across clinically relevant drinking subgroups (e.g., unhealthy drinkers [UHDs] and those drinking below unhealthy drinking thresholds [non-UHDs]). Study 1 evaluated the factor structure and measurement invariance of the AUDIT across UHDs/non-UHDs (n = 1,350, 79.3 % female, 31.2 % UHDs). Study 2 validated a statistically derived 8-item AUDIT (AUDIT-8) in an independent sample of UHDs (n = 238, 49.2 % female). Confirmatory factor analysis examined factor structure and measurement invariance between UHDs/non-UHDs. Item response theory and differential item functioning evaluated sources of variance and removed problematic items, yielding the AUDIT-8. Predictive validity and test-retest reliability of the AUDIT-8 were examined. In study 2, convergent validity and factor structure of the AUDIT-8 were examined. A unidimensional AUDIT model fit best. Invariance models suggested differences across UHDs/non-UHDs. Items 1-3 (consumption) were most relevant for the non-UHDs. Items 4-8 (problems) were most relevant for UHDs; however, items 4-8 were not relevant and rarely endorsed among non-UHDs. Items 9-10 performed poorly in both groups. Test-retest reliability and predictive validity of AUDIT-8 was acceptable. In a second sample of UHDs, AUDIT-8 had a unidimensional structure and acceptable convergent validity with measures of consumption, affect and drinking motives. Results suggest that only items 1-3 of the AUDIT should be administered in screenings for UHD among university students, with items 4-8 administered among positive screens to confirm UHD. Items 9-10 should be removed. Implications for AUDIT-8 as a two-part screening tool are discussed.


Asunto(s)
Alcoholismo , Humanos , Femenino , Masculino , Alcoholismo/diagnóstico , Reproducibilidad de los Resultados , Psicometría , Análisis Factorial , Tamizaje Masivo , Encuestas y Cuestionarios , Consumo de Bebidas Alcohólicas
14.
J Proteomics ; 271: 104769, 2023 Jan 16.
Artículo en Inglés | MEDLINE | ID: mdl-36372392

RESUMEN

OBJECTIVE: This study aims to find new plasma biomarkers in early pregnancy. DESIGN: The original study enrolled 1219 pregnant women. We investigated protein expression profiles of placental tissues from women with GDM (n = 89) and normal glucose tolerance (NGT) (n = 83). Maternal plasma samples between two groups in early and middle pregnancy were used for validation of candidate biomarkers. METHODS: Differentially expressed proteins (DEPs) were identified by label-free quantitative proteomics from human placenta samples between two groups. Several DEPs were validated in plasma by Luminex assays. An automatic biochemical analyzer was used to detect blood lipid indexes. The associations of GAL-3BP with biochemical indicators were demonstrated by Pearson's correlation analysis. Binary logistic regression was used to model potential predictive indicators in early pregnancy of GDM. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic accuracy of the predictive model and the value of GAL-3BP. RESULTS: 123 DEPs were found in placenta involved in ribosomal function, pancreatic secretion, oxidative phosphorylation, and inflammatory signaling pathway. Plasma GAL-3BP are significantly higher in women with GDM than NGT in the first (p = 0.008) and second (p = 0.026) trimester, but C9 and VWF have no difference. The predictive value of GAL-3BP in the first trimester of pregnancy (AUC 0.64) is better than that in the second trimester (AUC 0.61), and combined predictive model of TG and GAL-3BP at early pregnancy has greater predictive and diagnostic value for GDM (AUC 0.69) than individual GAL-3BP (AUC 0.64). CONCLUSIONS: Plasma TG and GAL-3BP has good predictive and diagnostic value at early pregnancy, suggesting that these two indicators may be used as biomarkers for early prediction and diagnosis of GDM. SIGNIFICANCE: The advantage of this study is that circulating TG and GAL-3BP might differentiate the progress of women with GDM and normal glucose tolerance (NGT) at the early stage of pregnancy. It is the first study to consider the role of GAL-3BP as an early predictive biomarker in the development of GDM during the whole pregnancy. Another advantage is that volunteers in this study were recruited from two provinces in China to eliminate the impacts of environmental confounders. The similar changes of blood glucose/lipid indicators for women with GDM and NGT in both regions was found in the first and second trimester of pregnancy, which added to the reliability of analytical results.


Asunto(s)
Diabetes Gestacional , Femenino , Embarazo , Humanos , Diabetes Gestacional/diagnóstico , Diabetes Gestacional/metabolismo , Estudios de Casos y Controles , Reproducibilidad de los Resultados , Placenta/química , Biomarcadores/metabolismo , Glucemia/metabolismo , China , Lípidos
15.
Ultrasonics ; 128: 106888, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36402114

RESUMEN

BACKGROUND: Ultrasound neurostimulation (USNS) is a non-invasive neuromodulation technique that might hold promise for treating neuropsychiatric disorders with regards to its noninvasiveness, penetration depth, and high resolution. OBJECTIVE: We sought in this experimental study to provide detailed and optimized protocol and methodology for a successful ultrasonic neurostimulation of the Primary Motor Cortex (M1) in mice addressed to young researchers/students beginning their research in the field of ultrasonic neurostimulation and encountering practical challenges. METHODS: A 500 kHz single-element transducer was used for stimulating the primary motor cortex at different acoustic pressures in C57BL/6 mice at various anesthesia levels. To further illustrate the effect of anesthesia, real time visual observations of motor responses validated with video recordings as well as electromyography were employed for evaluating the success and reliability of the stimulations. RESULTS: Detailed experimental procedure for a successful stimulations including targeting and anesthesia is presented. Our study demonstrates that we can achieve high stimulation success rates (91 % to 100 %) at acoustic pressures ranging from 330 kPa to 550 kPa at anesthesia washout period. CONCLUSIONS: This study shows a reliable and detailed methodology for successful USNS in mice addressed to beginners in ultrasonic brain stimulation topic. We showed an effective USNS protocol. We offered a simple and consistent non-invasive technique for locating and targeting brain zones. Moreover, we illustrated the acoustic pressure and stimulation success relationship and focused on the effect of anesthesia level for successful stimulation.


Asunto(s)
Corteza Motora , Animales , Ratones , Ratones Endogámicos C57BL , Reproducibilidad de los Resultados , Ultrasonido , Acústica
16.
Anal Chim Acta ; 1237: 340581, 2023 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-36442935

RESUMEN

In this study, covalent organic framework@MXene (COF@Ti3C2Tx) composites were synthesized and employed as solid phase microextraction coatings for isolation of polycyclic aromatic hydrocarbons (PAHs) from honey samples. The developed composites possessed unique structural characteristics and multiple interactions (π-π and hydrophobic interaction, etc.), which provided them with excellent extraction capacity towards the targeted PAHs. Under optimized conditions, the resultant COF@Ti3C2Tx-coating fiber-based HS-SPME/GC-FID method exhibited a wide linear range (2.0-2000 ng g-1), good linearities (r > 0.9964), and low limits of detection (0.20-0.60 ng g-1). The recoveries of PAHs in the honey samples were in the range of 73.2-112%, with RSDs less than 9.4%. In addition, the findings showed that the composite-based fiber offers a long lifetime, high enhancement factors (483-598), and excellent fiber-to-fiber reproducibility (RSDs <10.5%, n = 3). This work not only details the development of a multi-functional composite, but it also presents an effective strategy for the determination of trace PAHs in complex food matrix.


Asunto(s)
Miel , Estructuras Metalorgánicas , Hidrocarburos Policíclicos Aromáticos , Microextracción en Fase Sólida , Reproducibilidad de los Resultados , Titanio
17.
Anal Chim Acta ; 1237: 340598, 2023 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-36442937

RESUMEN

All-solid-state potentiometric sensors that are easily miniaturized and arrayed are widely used in home diagnostics. However, changes in sample matrix compositions tend to affect the basic potential of the potentiometric sensor, and pH of sample could change the response slope, thus affecting the detection reliability. This study takes the detection of glucose in urine as a model to increase the reliability of potentiometric sensors in home detection. PtAu/CNTs nanozyme modified by diboronic acid has been designed, showing better catalytic selectivity for glucose by experiments and theoretical calculations. Moreover, glucose electrode group in a multi-calibration glucose potentiometric sensing array can realize the basic potential calibration of sensing channel by the calibration channel. Meanwhile, the pH electrode group can not only measure the urine pH, but also calibrate the response slope of the glucose electrode group, thus improving the reliability of home detection.


Asunto(s)
Glucosuria , Humanos , Calibración , Reproducibilidad de los Resultados , Potenciometría , Glucosa
18.
Anal Chim Acta ; 1237: 340555, 2023 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-36442952

RESUMEN

Colorimetric sensing is a low-cost and visual method for food freshness monitoring. We prepared a Vanadium-Iron Prussian blue analogue (V1/5Fe(CN)6 PBA) impregnated with insulin (INS), as a peroxidase-like mimetic probe toward 3,3'5,5'-tetramethylbenzidine (TMB) oxidation and colorimetric monitoring of fish freshness. A specific chemical reaction between putrescine as spoilage marker and the Vanadium-Iron V1/5Fe(CN)6PBA impregnated with insulin (V1/5Fe(CN)6PBA/INS) induces a decreasing peroxidase-like activity of the V1/5Fe(CN)6PBA/INS, generating lower amounts of oxidized products of TMB. The proposed bio-strategy achieved highly sensitive responses in the range of 30 nM to 12 µM toward putrescine with a detection limit of 9.0 nM and outstanding stability, reproducibility, and selectivity. The freshness of five different types of fish samples determined and produces a noticeable color change consistent with the stale fish around the 10 µM, which can warn the oldness of the fish. This new strategy of integration between peptide and multi-metal PBA with intensified enzyme-like mimetics activity may be highly meaningful for thoroughly monitoring the amount of fish freshness.


Asunto(s)
Colorimetría , Putrescina , Animales , Reproducibilidad de los Resultados , Vanadio , Peces , Péptidos , Insulina , Peroxidasas , Hierro
19.
Sci Total Environ ; 858(Pt 2): 159697, 2023 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-36334664

RESUMEN

The growing increase in groundwater (GW) salinization in the coastal aquifers has reached an alarming socio-economic menace in Saudi Arabia and various places globally due to several natural and anthropogenic activities. Hence, evaluating the GW salinization is paramount to safeguarding the water resources planning and management. This study presents three different scenarios viz.: real field investigation, experimental laboratory analysis (using ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP-MS), etc.), and artificial intelligence (AI) based metaheuristic optimization (MO) algorithms in Saudi Arabia. The main purpose of this study is to validate the obtained experimental-based analysis using hybrid MO techniques comprising of adaptive neuro-fuzzy inference system (ANFIS) hybridized with genetic algorithm (GA), particle swarm optimization (PSO), and biogeography-based optimization (BBO) for identification of GW salinization in the coastal region of eastern Saudi Arabia. Additionally, ArcGIS 10.3 software generates the prediction map based on ANFIS-GA, ANFIS-PSO, and ANFIS-BBO. Feature selection was assessed using the PSO algorithm, and four indices evaluated the estimated models, namely, root mean square error (RMSE), mean absolute error (MAE), mean absolute percentage error (MAPE), and standard deviation (SD). The simulated results are based on three variable input combinations, which showed that the ANFIS-PSO (MAE = 0.00439) algorithm had the highest accuracy (99 %), followed by the ANFIS-GA (MAE = 0.00767) and ANFIS-BBO (MAE = 0.0132) algorithms. Besides, Ca2+, Na+, Mg2+, and Cl- were the most influential parameters. The accuracy also demonstrated the potential reliability of MO algorithms based on spatial distribution mapping. The employed approach proved to be merit and reliable tool for water resources decision-makers in the coastal aquifer of Saudi Arabia. This approach is believed to improve water scarcity as one of the essential targets for Goal 6 of Sustainable Development Vision 2030 and the Kingdom in general.


Asunto(s)
Lógica Difusa , Agua Subterránea , Inteligencia Artificial , Heurística , Arabia Saudita , Reproducibilidad de los Resultados , Algoritmos
20.
Cornea ; 42(1): 74-79, 2023 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-36197337

RESUMEN

PURPOSE: Dystrophic epidermolysis bullosa (DEB) is a devastating condition that causes painful corneal abrasions and vision loss. Epidermolysis Bullosa Eye Disease Index (EB-EDI) for the first time captures and quantifies EB-specific assessment of ocular symptoms and activities of daily living scales. This survey will become critical in developing new interventions on patients' quality of life. METHODS: Three-part set of the EB-EDI baseline, EB-EDI interval, and Ocular Surface Disease Index (OSDI) survey was distributed to 92 patients with DEB who previously reported eye symptoms on previous surveys. It was then posted online through several EB patient organizations. We compared the EB-EDI with the gold standard OSDI and examined the repeatability of the EB-EDI over a 7- to 15-day interval. RESULTS: Of the 45 individuals who initially responded, 30 of 45 (67%) completed the surveys sent 7 to 15 days later. The age of participants ranged from 6 to 51 years (mean 21 ± 15 years), and 60% (18 of 30) of participants were younger than 18 years. The overall Cronbach alpha values for the subscales of EB-EDI baseline and interval tools presented a good internal consistency (≥0.7). From 2 visits, the domain scores of EB-EDI baseline (0.94) and interval tools (0.83) were shown to have excellent test-retest reliability (intraclass correlation coefficient >0.8). By comparison, OSDI had the intraclass correlation coefficient score of 0.72 ± 0.11. The convergent validation analysis showed that correlations between the domain scores of EB-EDI baseline and interval tools and the subscales of the OSDI reached the hypothesized strength. CONCLUSIONS: Based on a 30-person repeated-measures study, we found that the EB-EDI has excellent reliability and validity specifically in patients with DEB.


Asunto(s)
Epidermólisis Ampollosa Distrófica , Epidermólisis Ampollosa , Oftalmopatías , Humanos , Niño , Adolescente , Adulto Joven , Adulto , Persona de Mediana Edad , Epidermólisis Ampollosa Distrófica/complicaciones , Epidermólisis Ampollosa Distrófica/diagnóstico , Reproducibilidad de los Resultados , Calidad de Vida , Actividades Cotidianas , Epidermólisis Ampollosa/complicaciones , Oftalmopatías/etiología
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