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1.
Food Chem ; 366: 130563, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34289441

RESUMEN

Herein, a simple, low-cost, environment-friendly strategy was proposed to prepare the composite of three-dimensional hierarchical porous carbon and chitosan, which was applied to modify the glass carbon electrode to fabricate an electrochemical sensor for the determination of niclosamide. The three-dimensional porous carbon with interconnected conductive network, high surface area, and self-generated oxygen-containing functional groups was prepared by salt-templating method with glucose as carbon source and eutectic mixture of LiBr/KBr as both activating and pore-forming agent. During the subsequent ultrasonic process, chitosan with excellent filming property, strong adsorption ability, and good dispersibility was successfully decorated on the obtained porous carbon to further enhance the determination performance of niclosamide. Benefitting from the multi-functional integration of three-dimensional hierarchical porous carbon and chitosan, the fabricated sensor presented a low limit of detection (6.7 nM) in the linear concentration range from 0.01 to 10 µM. Moreover, the fabricated sensor could show good repeatability, reproducibility, stability, and selectivity. Most important, the decent practicability for the detection of niclosamide was obtained in different food samples with low relative standard deviation and satisfactory recoveries. This work provides a very valuable reference for the sensitive determination of niclosamide in food samples.


Asunto(s)
Quitosano , Carbono , Técnicas Electroquímicas , Electrodos , Niclosamida , Porosidad , Reproducibilidad de los Resultados
2.
Talanta ; 236: 122837, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34635227

RESUMEN

A quartz crystal microbalance (QCM) sensor was developed in this study with the vegetable oil from olive (OLV-QCM) to detect an important volatile organic compound, ß-pinene in Indian cardamom. Hydrophobic vegetable oil from olive, which contains oleic acid and omega-9, a monounsaturated fatty acid was found to be suitable for binding ß-pinene through non-covalent bonds. The fabricated QCM sensor coating was examined with the field emission scanning electron microscope (FESEM) and Fourier-transform infrared spectroscopy (FTIR) to determine its surface morphology and chemical compositions. The sensitivity, reproducibility, repeatability, and reusability were studied for the developed sensor. Notably, the sensor was observed to be highly selective towards ß-pinene as compared to the other volatile components present in cardamom. The limit of detection (LOD) and limit of quantitation (LOQ) parameters were determined as 5.57 mg L-1 and 18.57 mg L-1, respectively. Moreover, the adsorption isotherm models of the sensor were studied to validate the physical adsorption affinity towards ß-pinene applying Langmuir, Freundlich, and Langmuir-Freundlich isotherm models. The sensor showed a correlation factor of 0.99 with the peak area percentage of gas chromatography-mass spectrometry (GC-MS) analysis for ß-pinene in cardamom samples. The sensor was prepared with natural vegetable oil, unlike health hazard chemicals. In addition to this, the low-cost, easy fabrication process ensured the suitability of the sensor for practical deployment.


Asunto(s)
Elettaria , Impresión Molecular , Monoterpenos Bicíclicos , Aceites Vegetales , Polímeros , Tecnicas de Microbalanza del Cristal de Cuarzo , Reproducibilidad de los Resultados
3.
Talanta ; 236: 122856, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34635240

RESUMEN

In this study, a first attempt for isolating and determining (characterising) background levels of titanium dioxide nanoparticles (TiO2 NPs) in seaweed has been developed by using single particle inductively coupled plasma - mass spectrometry (SP-ICP-MS). Seaweeds were processed using an optimised ultrasound assisted extraction (UAE) procedure based on tetramethylammonium hydroxide (TMAH) before dilution and SP-ICP-MS analysis. The effect of the TMAH percentage in the extracting solution, as well as the volume of extracting solution and sonication (extraction) time, has been fully assessed. Additional experiments also showed that TiO2 NPs were quantitatively released from the seaweed matrix in one UAE step since the analysis of residues gave TiO2 NPs concentrations lower than the limit of quantification (LOQ) of the method. Validation of the method with 50 and 100 nm TiO2 NPs (10 µg L-1 as Ti) showed good analytical recovery (115% and 112% for 50 and 100 nm TiO2 NPs, respectively), and good reproducibility (2% for size and 16% for number of TiO2 NPs). Experiments regarding TiO2 NPs stability showed that the extracted NPs are stable since there were not changes on the number of TiO2 NPs and TiO2 NPs size distributions when exposing TiO2 NPs standards to the optimised extractive conditions.


Asunto(s)
Nanopartículas , Algas Marinas , Espectrometría de Masas , Reproducibilidad de los Resultados , Titanio
4.
Talanta ; 236: 122858, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34635242

RESUMEN

Chemiluminescence signal amplification (CLSA) is of huge interest because of its sensitive detection in various applications such as food analysis, biomedical diagnosis and environmental monitoring. Due to this, there is a manifold attention to develop rapidly prototyped and miniaturized devices for CLSA. In this context, herein, a novel CLSA approach is demonstrated on a 3D printed microfluidic paper-based analytical device (µPADs), fabricated using Fused deposition modeling (FDM) printing technology. Influence of working temperature, ranging 30 °C-110 °C, on CL signal generation from well-established Luminol/Co+2 - H2O2 reaction was analyzed using a screen-printed flexible heater onto the 3D printed reaction platform. A smartphone-based capturing/detection system provided the amenability for a point-of-care testing system. For the first time, strong and stable CLSA was found with about 255% ± 5% increase in its signal intensity without using any additional external enhancers. The on-site working temperature was directly in proportional to the intensity of CL signal generated from Luminol/Co+2 - H2O2 reaction under optimum conditions, wherein the device had a wide linear range from 50 nM to 1 µM with a detection limit of 35 nM for H2O2 detection. The reliability of the developed amplification method was tested for practicability to detect the concentration of H2O2 in milk as real sample analysis. Overall, such CLSA mechanism in miniaturized µPADs will have strong potential for multiple CL based detection and monitoring application.


Asunto(s)
Luminiscencia , Teléfono Inteligente , Calefacción , Peróxido de Hidrógeno , Mediciones Luminiscentes , Microfluídica , Peróxidos , Reproducibilidad de los Resultados
5.
Talanta ; 236: 122879, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34635259

RESUMEN

We present a sensitive label-free surface enhanced Raman spectroscopy (SERS) method for the discrimination between the recombinant and endogenous human Erythropoietin (EPO) isoforms. The proposed methodology comprises a lectin-functionalised extractor chip for the extraction of the recombinant human EPO (rhuEPO) and the endogenous EPO (enEPO) from blood plasma. The disulfide bond molecular structure of the purified isoforms was modified to chemisorb the biomolecules onto a SERS substrate in a unified orientation, thus maximizing the reproducibility and sensitivity of the SERS measurements. The acquired SERS spectra of the EPO isoforms showed diagnostic Raman bands that allowed for the discrimination between rhuEPO and enEPO. The method was also used for the SERS quantification of rhuEPO and enEPO down to 0.1 pM and 0.5 pM, respectively. The SERS determination of the protein isoforms was cross validated against ELISA. The new SERS method has strong potential for the rapid screening of rhuEPO doping in athletes and for the therapeutic drug monitoring of rhuEPO treatment in cancer patients.


Asunto(s)
Eritropoyetina , Espectrometría Raman , Humanos , Isoformas de Proteínas , Proteínas Recombinantes , Reproducibilidad de los Resultados
6.
Talanta ; 236: 122880, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34635260

RESUMEN

A novel aptamer-functionalized metal-organic framework nanofibrous composite (viz. PAN/UiO@UiO2-N3-aptamer) with a high aptamer coverage density was proposed based on the electrospinning and seeded growth method, and used for specific affinity recognition of trace Microcystin-LR (MC-LR). Heterobifunctional ligand was used to modify the metal-organic framework nanoparticles (MOF NPs) surface, which could passivate the MOF surface with respect to unmodified DNA, followed by coupling massive aptamers on MOF of the solid-phase microextraction (SPME) fiber using click chemistry. Characterizations including morphology, spectra analysis, mechanical stability, binding capacity and specificity were fulfilled. Applied to the analysis of MC-LR, the good selective and sensitive recognition were obtained with the detection limit as low as 0.003 ng/mL, which was better than most non-specific SPME or solid-phase extraction (SPE) protocols. The stability and reproducibility were acceptable, and the intra-day, inter-day and column-to-column relative standard deviations (RSDs) for the recovery of MC-LR were gained in the range from 2.5% to 14.3%, respectively. Satisfactory recoveries of MC-LR in environmental water samples were measured as 96.3 ± 4.7% - 98.9 ± 2.7% (n = 3) in tap water, 94.4 ± 2.5% - 96.1 ± 3.5% (n = 3) in pond water, and 97.0 ± 2.1% - 97.9 ± 3.1% (n = 3) in river water, respectively. This work demonstrated that the electrospun nanofibrous composite with massive aptamers would be a better alternative for ultra-trace MC-LR detection with good selectivity, matrix-resistance ability and high resolution.


Asunto(s)
Aptámeros de Nucleótidos , Estructuras Metalorgánicas , Nanofibras , Toxinas Marinas , Microcistinas , Reproducibilidad de los Resultados
7.
Food Chem ; 368: 130761, 2022 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-34392119

RESUMEN

Raffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.


Asunto(s)
Fabaceae , Cromatografía Líquida de Alta Presión , Cromatografía Liquida , Espectrometría de Masas , Oligosacáridos , Reproducibilidad de los Resultados
8.
J Hazard Mater ; 421: 126705, 2022 01 05.
Artículo en Inglés | MEDLINE | ID: mdl-34315017

RESUMEN

In this study, a novel method-atom surface fragment contribution (ASFC)-was proposed for assessing the properties of compounds. We developed a predictive model using the ASFC method based on the sigma surface areas (Sσ-surface) of fragments/groups for estimating the toxicity of ILs. A toxicity dataset of 140 ILs towards leukemia rat cell line (ICP-81) was gathered and employed to train and validate models. The Sσ-surface values of atoms in each group were firstly calculated from the COSMO profiles of cations and anions for ILs. Then the Sσ-surface values of 26 groups were obtained and used as input descriptors for modelling. The R2 and MSE of the built ASFC model were 0.924 and 0.071, respectively. Results indicate that the ASFC model developed by the new approach possesses great accuracy and reliability. In total, the ASFC method has extensive potential for the application of estimating diverse properties of ILs and other compounds due to its remarkable advantages.


Asunto(s)
Líquidos Iónicos , Animales , Aniones , Cationes , Líquidos Iónicos/toxicidad , Relación Estructura-Actividad Cuantitativa , Ratas , Reproducibilidad de los Resultados
9.
Food Chem ; 369: 130801, 2022 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-34450514

RESUMEN

Volatile organic compounds (VOCs) emitted by food products are decisive for the perception of aroma and taste. The analysis of gaseous matrices is traditionally done by detection and quantification of few dozens of characteristic markers. Emerging direct injection mass spectrometry technologies offer rapid analysis based on a soft ionisation of VOCs without previous separation. The recent increase of selectivity offered by the use of several precursor ions coupled with untargeted analysis increases the potential power of these instruments. However, the analysis of complex gaseous matrix results in a large number of ion conflicts, making the quantification of markers difficult, and in a large volume of data. In this work, we present the exploitation of untargeted SIFT-MS volatile fingerprints of ewe PDO cheeses in a real farm model, using mixOmics methods allowing us to illustrate the typicality, the manufacturing processes reproducibility and the impact of the animals' diet on the final product.


Asunto(s)
Queso , Compuestos Orgánicos Volátiles , Animales , Queso/análisis , Femenino , Espectrometría de Masas , Odorantes , Reproducibilidad de los Resultados , Ovinos , Compuestos Orgánicos Volátiles/análisis
10.
Food Chem ; 369: 130898, 2022 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-34455326

RESUMEN

This review summarizes the use of spectroscopic processes-based analytical tools coupled with chemometric techniques for the identification of adulterants in edible oil. Investigational approaches of process analytical tools such asspectroscopy techniques, nuclear magnetic resonance (NMR), hyperspectral imaging (HSI), e-tongue and e-nose combined with chemometrics were used to monitor quality of edible oils. Owing to the variety and intricacy of edible oil properties along with the alterations in attributes of the PAT tools, the reliability of the tool used and the operating factors are the crucial components which require attention to enhance the efficiency in identification of adulterants. The combination of process analytical tools with chemometrics offers a robust technique with immense chemotaxonomic potential. These involves identification of adulterants, quality control, geographical origin evaluation, process evaluation, and product categorization.


Asunto(s)
Alimentos , Aceites Vegetales , Espectroscopía de Resonancia Magnética , Reproducibilidad de los Resultados
11.
Food Chem ; 369: 130944, 2022 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-34464834

RESUMEN

A headspace-solid phase microextraction - gas chromatography-flame ionisation detector (HS-SPME-GC/FID) method was developed for the simultaneous determination of furan, 2-methylfuran and 2-furaldehyde in thermally processed Mopane worms, corn, and peanuts. The optimal HS-SPME conditions with polydimethylsiloxane/carboxen/divinylbenzene (PDMS/CAR/DVB) fiber were 30 °C, 40 min and 600 rpm stirring speed. The recoveries, detection and quantification limits for the analytes in food samples were 67-106%, 0.54-3.5 µg kg-1, and 1.8-12 µg kg-1, respectively. These results showed that the developed method was accurate, reproducible, and sensitive for the determination of furan, 2-methylfuran and 2-furaldehyde in complex food matrices with limited interference from other components. The optimised analytical method was applied for monitoring the presence of the furanic compounds in heat-processed South African foods. Although 2-furaldehyde was not detected in food samples, the maximum concentrations of 24 and 95 µg kg-1 were found for furan and 2-methylfuran, respectively.


Asunto(s)
Microextracción en Fase Sólida , Zea mays , Arachis , Cromatografía de Gases , Furaldehído , Reproducibilidad de los Resultados
12.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120267, 2022 Jan 05.
Artículo en Inglés | MEDLINE | ID: mdl-34419828

RESUMEN

In this paper, a fast and efficient analytical strategy was proposed that chemometrics assisted with excitation-emission fluorescence matrices was used to quantify carbaryl (CAR) and thiabendazole (TBZ) in peach, soil and sewage. Even if there are serious overlapped peaks and unknown interferences in fluorescence analysis, the second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm can be used to analyze CAR and TBZ in peach, soil and sewage. The recoveries of CAR and TBZ in peach are 110.4% and 99.7% and their standard deviations are lower than 2.1% and 0.3%, respectively. In addition, the accuracy of the method was assessed with figures of merit as well as intra-day and inter-day precision. The limit of detection, the limit of quantitation of CAR and TBZ in peach are 1.2 ng mL-1 and 0.3 ng mL-1, 3.5 ng mL-1 and 0.8 ng mL-1, respectively. And their root-mean-square error of prediction are 17.0 ng mL-1 and 5.0 ng mL-1 and there are high sensitivity and selectivity in this method. Meanwhile, the results obtained by ATLD algorithm were compared with those obtained by the self-weighted alternate trilinear decomposition algorithm (SWATLD) and the parallel factor analysis (PARAFAC) algorithm, and statistical methods such as the t-test, F-test and the elliptic joint confidence region were used to evaluate for analysis. There were no significant differences among these methods. At last, high performance liquid chromatography-fluorescence detector (HPLC-FLD) was used to evaluate the accuracy and reliability of the proposed method. These results are satisfactory and indicate that the proposed method can be used for accurate and rapid determination of pesticides in complex systems.


Asunto(s)
Carbaril , Tiabendazol , Algoritmos , Calibración , Cromatografía Líquida de Alta Presión , Reproducibilidad de los Resultados , Espectrometría de Fluorescencia
13.
J Environ Manage ; 301: 113830, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34600425

RESUMEN

Stormwater harvesting is an effective measure to mitigate flooding risk and pollutant migration in our urban environment with the continuously increasing impermeable faction. Treatment of harvested stormwater also provides the fit-for-purpose water sources as an alternative to potable water supply ensuring the reliability and sustainability of the water management in the living complex. In order to provide the water management decision-maker with a broad range of related technology database and to facilitate the implementation of stormwater harvesting in the future, a comprehensive review was undertaken to understand the corresponding treatment performance, the applicable circumstances of current stormwater treatment and harvesting technologies. Technologies with promising potential for stormwater treatment were also reviewed to investigate the feasibility of being used in an integrated process. The raw stormwater quality and the required quality for different levels of stormwater reuses (irrigation, recreational, and potable) were reviewed and compared. The required level of treatment is defined for different 'fit-for-purpose' uses of harvested stormwater. Stormwater biofilter and constructed wetland as the two most advanced and widely used stormwater harvesting and treatment technologies, their main functionality, treatment performance and adequate scale of the application were reviewed based on published peer-reviewed articles and case studies. Excessive microbial effluent that exists in stormwater treated using these two technologies has restricted the stormwater reuse in most cases. Water disinfection technologies developed for wastewater and surface water treatment but with high potential to be used for stormwater treatment have been reviewed. Their feasibility and limitation for stormwater treatment are presented with respect to different levels of fit-for-purpose reuses. Implications for future implementation of stormwater treatment are made on proposing treatment trains that are suitable for different fit-for-purpose stormwater reuses.


Asunto(s)
Purificación del Agua , Abastecimiento de Agua , Lluvia , Reproducibilidad de los Resultados , Aguas Residuales
14.
J Environ Manage ; 301: 113900, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34638041

RESUMEN

This paper introduces a new framework to evaluate the resilience of lakes under climatic and anthropogenic droughts. The proposed hierarchical structure of criteria for assessing lake's resilience has four levels. The first level includes several indices such as long-term resilience, reliability, and implementation cost. In the second to fourth levels, four main resilience-based criteria (i.e. robustness, resourcefulness, redundancy, and rapidity) and some qualitative and quantitative sub-criteria are defined considering the factors affecting the ecological condition of lakes. To quantify the time series of the sub-criteria, a coupled SWAT-MODSIM-based simulation model has been applied. Also, the values of criteria and sub-criteria have been aggregated using the Evidential Reasoning (ER) approach. After estimating the annual resilience time series, three resilience indices, namely the recovery time (Tr), loss of resilience (LOR), and final resilience (Resf), have been calculated. The normalized values of these indices and reliability criteria have been aggregated to evaluate the overall performance of lake restoration scenarios. To show the applicability of the proposed methodology, the Zarrinehrud river basin and Lake Urmia have been selected as the case study. As one of the largest hypersaline lakes globally, Lake Urmia suffers from drastic changes in its water body and a high level of salinization. Also, the Zarrinehrud river basin, located in the southeastern of Urmia Lake, is the most significant sub-basin of the lake and is responsible for supplying 41% of the total annual inflow of the lake. The restoration scenarios of Lake Urmia have been assessed from 2019 to 2049. Eventually, the most effective scenario, which has an average overall performance of 0.72, the implementation cost of 17.1 million dollars, and the uncertainty band of 0.05, has been selected.


Asunto(s)
Sequías , Lagos , Monitoreo del Ambiente , Reproducibilidad de los Resultados , Ríos , Incertidumbre
15.
Food Chem ; 368: 130812, 2022 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-34419800

RESUMEN

Blueberry aroma is one of the most important quality traits that influences consumer purchasing decisions. This study aimed to optimize and validate a solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) method for the quantification of 73 volatile compounds in northern highbush blueberries. A SPME extraction of blueberries with water and specific proportions of sodium chloride, citric acid, and ascorbic acid, for 60 min at 50 °C using a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was optimal. The method was validated for sensitivity, reproducibility, linearity, and accuracy, and used to quantify volatile compounds through matrix-matched calibration curves in six blueberry cultivars ('Duke', 'Draper', 'Bluecrop', 'Calypso', 'Elliott', and 'Last Call'). Terpenes represented the most abundant volatile fraction, followed by aldehydes and alcohols. Linalool and 2-(E)-hexenal were key compounds that differentiated blueberry cultivars via Principal Component Analysis (PCA). Enantiomeric analyses revealed an excess of (-)-limonene, (-)-α-pinene, and (+)-linalool for all cultivars with potential impacts on the blueberry aroma.


Asunto(s)
Arándanos Azules (Planta) , Compuestos Orgánicos Volátiles , Cromatografía de Gases y Espectrometría de Masas , Odorantes/análisis , Reproducibilidad de los Resultados , Microextracción en Fase Sólida , Compuestos Orgánicos Volátiles/análisis
16.
Food Chem ; 366: 130575, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34293546

RESUMEN

Two bimetallic CoNi-based metallo-covalent organic frameworks (MCOFs) were prepared and explored as the sensitive platforms of impedimetric aptasensors for efficient detection of tobramycin (TOB). The two CoNi-MCOFs were constructed using metallophthalocyanine tetra-amine (MPc-TA, M = Co2+ or Ni2+) and 4,4'-(1,10-phen-anthroline-2,9-diyl) dibenzaldehyde (PTD) as building units and further coordinating to the secondary metal ions (Ni2+ or Co2+) by phenanthroline. Interestingly, the immobilization ability of CoPc-TA-PTD(Ni) to TOB-targeted aptamer is higher than that of NiPc-TA-PTD(Co) due to its stronger binding interactions to aptamer. As a result, the CoPc-TA-PTD(Ni)-based aptasensor shows the superior TOB detection ability, giving a low detection limit of 0.07 fg mL-1 and satisfied sensing performances, such as high selectivity, good reproducibility, and excellent stability. Also, the aptasensor shows the acceptable applicability for detecting TOB in milk or chicken egg. This MCOFs-based sensing strategy could be extensively applied to detect other analytes by anchoring the corresponding probes.


Asunto(s)
Aptámeros de Nucleótidos , Técnicas Biosensibles , Estructuras Metalorgánicas , Técnicas Electroquímicas , Límite de Detección , Reproducibilidad de los Resultados , Tobramicina
17.
Food Chem ; 367: 130659, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34343800

RESUMEN

This study presents a sensitive approach for electrochemical determination of 5-hydroxymethylfurfural (5-HMF) in food. The electrochemical sensor was fabricated on a copper electrode (CuE) modified with co-electrodeposited Cu-Ni bimetallic particles. This sensor, fabricated by 30 cycles of cyclic voltametric scanning with a scan rate of 50 mV s-1, exhibits good electrocatalytic ability to 5-HMF oxidation. Under the optimal conditions, linear scan voltammetry (LSV) and chronoamperometry were conducted for the determination of 5-HMF. The results of LSV show that a linear dependency within the 0.4-10 mM range with a detection limit (LOD) of 3.51 µM (S/N = 3) was achieved, while a linear range in 1 × 10-4-11 mM with a LOD of 0.043 µM (S/N = 3) was obtained by chronoamperometric measurement. The electrochemical sensor was finally applied in determination of 5-HMF in various foods, and the reliability and accuracy of the method were assessed by adopting an UV method as a standard method. Results show that the concentrations of 5-HMF in real samples are close to those measured by the standard method. In addition, standard addition method was further performed to evaluate the accuracy of our approach. The recoveries ranged from 90.0% to 110.0% are calculated, demonstrating good accuracy of the electrochemical sensor.


Asunto(s)
Cobre , Técnicas Electroquímicas , Electrodos , Furaldehído/análogos & derivados , Límite de Detección , Reproducibilidad de los Resultados
18.
Food Chem ; 367: 130676, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34365250

RESUMEN

Antimony(III) is a rare electroactive specie present on Earth, whose concentration is not typically determined. The presence of high concentrations of antimony is responsible for a variety of diseases, which makes it desirable to find convenient and reliable methods for its determination. We have developed a convenient glassy carbon modified electrode with electroreduced graphene oxide GC/rGO for the first time determination of Sb(III) in commercial lettuce, celery, and beverages. The surface of the electrode was characterized by scanning electron microscopy (SEM) and cyclic voltammetry, indicating a heterogeneous and rough surface with a real area of 0.28 cm2, which is ~2.5 times the area of GC. The optimal chemical and electrochemical parameters used were: sodium acetate buffer (pH = 4.3), an accumulation potential of -1.0 V and an accumulation time of 150 s. The analytical validation was developed evaluating the linear range (10-60 µg L-1), limit of detection (2.5 µg L-1), accuracy, repetibility and reproducibility with satisfactory results (relative standard deviation (RSD) values lower than 10%). All the analyzes performed in real samples by stripping voltammetry were compared with GF-AAS, showing statistically similar values, demonstrating that GC/rGO could be effectively applied in the analysis of food samples.


Asunto(s)
Grafito , Antimonio/análisis , Bebidas , Electrodos , Plantas Comestibles , Reproducibilidad de los Resultados
19.
Food Chem ; 367: 130727, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34371276

RESUMEN

An electrochemical sensor was fabricated by modifying nanoporous gold (NPG)-coated glassy carbon electrode (NPG/GCE) with functionalized graphene oxide /chitosan/ionic liquid nanocomposites (fGO/CS/IL). The introduction of ionic liquid (IL) and chitosan (CS) induced higher dispersibility of functionalized graphene oxide (fGO), and was beneficial for the combination of fGO/CS/IL with NPG/GCE. As a result of the synergistic effect of NPG and fGO/CS/IL, the resulted functionalized graphene oxide/chitosan/ionic liquid nanocomposites/nanoporous gold /glassy carbon electrode (fGO/CS/IL/NPG/GCE) showed the highest redox peak current response signal of Amaranth (E123) due to ultrahigh surface area, electronic conductivity as well as the improvement of the surface structure. Under optimized conditions, the enhanced peak currents represented excellent analytical performance for detection of Amaranth in the concentration range from 8.0 to 1200.0 nM. Meanwhile, the fGO/CS/IL/NPG/GCE presented satisfactory sensitivity and selectivity, excellent reproducibility, and long-time stability. For practical applications, the fGO/CS/IL/NPG/GCE was validated for the determination of Amaranth in three types of drinks with satisfactory results.


Asunto(s)
Técnicas Biosensibles , Quitosano , Grafito , Líquidos Iónicos , Nanocompuestos , Nanoporos , Técnicas Electroquímicas , Electrodos , Oro , Reproducibilidad de los Resultados
20.
Food Chem ; 367: 130714, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34388632

RESUMEN

The following qualitative screening and quantitative determination of 201 pesticides present a detailed optimization making use of design of experiments (DoE). The post-targeted screening took advantage of an upgradeable database to extend the survey further to perform a standard-free novel quantitation by liquid chromatography high resolution mass spectrometry (LC-HRMS). The fine tuning of instrumental parameters allowed the best method selectivity, sensitivity and reproducibility for the compounds under study. Among the factors considered, the heated electrospray source (HESI), the sample composition and the MS/MS acquisition modes were assessed, including the all-ion fragmentation (AIF) mode and different versions of the variable data independent acquisition (vDIA). The results of the optimization showed that selected HESI conditions, a methanol/water (1:1) sample composition and a vDIA acquisition method increased the signal for most compounds with a remarkable benefit on signal shape and intensity. The method showed adequate analytical performance when assessed in local produce.


Asunto(s)
Residuos de Plaguicidas , Plaguicidas , Cromatografía Líquida de Alta Presión , Cromatografía Liquida , Frutas/química , Residuos de Plaguicidas/análisis , Plaguicidas/análisis , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem , Verduras
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