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1.
Food Chem ; 335: 127602, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32739807

RESUMEN

Bioactive phenolics primarily contribute to versatile health benefits of pigeon pea. For the first time, an UPLC-QqQ-MS/MS method was developed for the quantitative analysis of eleven bioactive phenolic compounds in pigeon pea natural resources (seeds, leaves, and roots) and in vitro cultures (calli and hairy roots). The proposed method could be achieved within 6 min of running time, and displayed the satisfactory linearity, sensitivity, precision, accuracy, and stability. According to analytical results, the distribution of eleven target compounds in different organs of pigeon pea was clarified. Also, it was surprisingly found that pigeon pea in vitro cultures exhibited superiority in contents of genistin and cajaninstilbene acid as compared with natural resources. Overall, the present work provided a rapid and sensitive analysis approach, which could be useful not only for quality control of pigeon pea natural resources, but also for applicability and safety evaluation of pigeon pea in vitro cultures.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Guisantes/química , Fenoles/análisis , Espectrometría de Masas en Tándem/métodos , Medicamentos Herbarios Chinos/química , Hojas de la Planta/química , Control de Calidad , Reproducibilidad de los Resultados , Semillas/química
2.
Food Chem ; 335: 127609, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32739808

RESUMEN

In this study, a fluorescence resonance energy transfer (FRET) immunoassay based on graphene oxide (GO) and up-converting nanoparticles (UCNPs) was established for rapid detection of imidacloprid, a commonly-used insecticide. Under 980 nm near-infrared light excitation, emission of UCNPs at 542 nm can be absorbed by the energy acceptor GO. The carboxyl-functionalized GO and UCNPs were coupled with competitive antigen and antibody against imidacloprid. After optimization, the FRET immunoassay showed a wide detection range of 0.08-50 ng/mL to imidacloprid, with cross-reaction toward other three neonicotinoids including imidaclothiz (74.4%), thiacloprid (36.9%) and clothianidin (31.9%). The average recoveries of spiked water, Chinese cabbage, cucumber, honey and tea samples were 76.8%-101.8%. The accuracy and reliability of the FRET immunoassay were verified by UPLC-MS/MS with a good correlation (R2 = 0.9816). In a summary, this study provides a sensitive and one-step method for monitoring imidacloprid residue in food and environmental samples within 1 h.


Asunto(s)
Grafito/química , Nanopartículas/química , Neonicotinoides/análisis , Nitrocompuestos/análisis , Cromatografía Liquida , Transferencia Resonante de Energía de Fluorescencia/métodos , Inmunoensayo/métodos , Límite de Detección , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem , Tiazoles
3.
Food Chem ; 335: 127596, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-32745840

RESUMEN

The ciprofloxacin (CIP) abuse has caused many problems threatening to human health. Here, we design the quantum dot microsphere (QDM) based immunochromatographic quantitative CIP test strip: when the sample under detection contains CIP, the QDM-monoclonal antibody (mAb) probes bound with the CIP and cannot be captured by CIP-bovine serum albumin (BSA) conjugation dispersed on the T lines, reducing the fluorescence intensities. These test strips can provide a low detection limit of 0.05 ng/mL and a wide linear detection range from 0.1 ng/mL to 100 ng/mL in high sensitivity and accuracy as well as good selectivity, reproducibility and stability. Moreover, a smartphone based test strip reader with the size of 85 mm × 48 mm × 44 mm is also fabricated using 3-D printing to automatically and quantitatively detect CIP. The whole process of CIP detection can be finished within 15 min, but only cost ~1 RMB (10 cents).


Asunto(s)
Antibacterianos/análisis , Ciprofloxacino/análisis , Inmunoensayo/instrumentación , Puntos Cuánticos/química , Animales , Anticuerpos Monoclonales/inmunología , Fluorescencia , Humanos , Límite de Detección , Microesferas , Impresión Tridimensional , Reproducibilidad de los Resultados , Albúmina Sérica Bovina/inmunología
4.
Sci Total Environ ; 750: 141692, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32846246

RESUMEN

A portable, cheap and sensitive paper type electrochemical immunosensor was developed with conductive nanobiochar paper as the conductive layer and utilized for sensitive detection of microcystin-LR (MCLR) toxin in water. The paper immunosensor was constructed by coating of highly conductive and dispersible nanobiochar particle (nBC) and anti-MCLR antibody on the filter paper via dipping-drying method. The presence of MCLR could be specifically quantified amperometrically by the nBC-paper immunosensor with the response time of less than 5 min, and the lowest detection limit of 17 pM (0.017 µg/L) was achieved. Moreover, the proposed immunosensor exhibited high selectivity, reproducibility and storage stability, and was also used for environmental water detection with satisfactory recovery. The successful fabrication of low cost and ubiquitous biochar based paper type electrochemical immunosensing system would have significant value for the development of highly cost-effective electrochemical device.


Asunto(s)
Técnicas Biosensibles , Técnicas Electroquímicas , Oro , Inmunoensayo , Límite de Detección , Microcistinas , Reproducibilidad de los Resultados
5.
Food Chem ; 334: 127601, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32712491

RESUMEN

Quantitative labeling of oil compositions has become a trend to ensure the quality and safety of blended oils in the market. However, methods for rapid and reliable quantitation of blended oils are still not available. In this study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to profile triacylglycerols in blended oils, and partial least squares regression (PLS-R) was applied to establish quantitative models based on the acquired MALDI-MS spectra. We demonstrated that this new method allowed simultaneous quantitation of multiple compositions, and provided good quantitative results of binary, ternary and quaternary blended oils, enabling good limits of detection (e.g., detectability of 1.5% olive oil in sunflower seed oil). Compared with the conventional GC-FID method, this new method could allow direct analysis of blended oils, analysis of one blended oil sample within minutes, and accurate quantitation of low-abundance oil compositions and blended oils with similar fatty acid contents.


Asunto(s)
Análisis de los Alimentos/métodos , Aceites Vegetales/análisis , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Cromatografía de Gases/métodos , Ácidos Grasos/análisis , Análisis de los Alimentos/estadística & datos numéricos , Contaminación de Alimentos/análisis , Análisis de los Mínimos Cuadrados , Aceite de Oliva/análisis , Aceites Vegetales/química , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/estadística & datos numéricos , Aceite de Girasol/análisis , Triglicéridos/análisis
6.
Food Chem ; 334: 127471, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32688174

RESUMEN

Optimal QuEChERS (quick, easy, cheap, effective, rugged and safe) conditions with good accuracy, repeatability and precision were established to rapidly extract the European Union (EU) priority polycyclic aromatic hydrocarbons (PAHs) from various food matrices (Category: Poultry and Meat, Fish and seafood, Grains, Soy beans and products, Root vegetables and Coffee). The QuEChERS conditions combined with the established high performance liquid chromatography-fluorescence detection conditions were used to rapidly determine the PAHs in 19 popular cooked foods in Taiwan and their corresponding original materials. These conditions also meet the EU and Taiwan Food and Drug Administration specifications. Charcoal grilled, gas stove grilled and smoked foods had higher PAHs contents, while fried and electric oven-baked/baked foods had lower PAHs contents. In addition to the effects of cooking methods, the contamination of original materials by PAHs in the environment should also have an important impact on the contents of PAHs in these cooked foods.


Asunto(s)
Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Hidrocarburos Policíclicos Aromáticos/análisis , Carbón Orgánico , Fraccionamiento Químico/métodos , Cromatografía Líquida de Alta Presión , Culinaria , Unión Europea , Cromatografía de Gases y Espectrometría de Masas/métodos , Límite de Detección , Carne/análisis , Reproducibilidad de los Resultados , Alimentos Marinos/análisis , Taiwán , Verduras/química
7.
Food Chem ; 334: 127436, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711262

RESUMEN

Milk samples (150 cow and 217 camel milk samples) were analyzed for protein, fat, lactose and total solids by near and mid-infrared transmission spectroscopy. Excellent positive correlations between the two methods were obtained for both types of milk (p < 0.001); for protein (r ≥ 0.96), fat (r ≥ 0.99), lactose (r = 0.82) and total solids (r = 0.90). The mean of the relative difference ((MIR values - NIR values)/0.5 (MIR values + NIR values) × 100%) for cow and camel milk were, for protein (+8.2 & +13.4%), fat (-9.3 & +0.9%), lactose (-5.4 &-0.7%) and total solids (-2.2 &-3.4%), respectively. The difference between the two methods may be due to the effects of differences in milk homogeneity, especially with respect to casein micelles and fat globules.


Asunto(s)
Análisis de los Alimentos/métodos , Lactosa/análisis , Proteínas de la Leche/análisis , Leche/química , Espectrofotometría Infrarroja/métodos , Animales , Calibración , Camelus , Caseínas/análisis , Bovinos , Grasas de la Dieta/análisis , Femenino , Reproducibilidad de los Resultados
8.
Food Chem ; 337: 127761, 2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-32777565

RESUMEN

Amino and thiolated aptamers are the main aptamers used to construct label-free electrochemical impedimetric aptasensors. In this study, the modification performance and electrochemical properties of amino aptamers and thiolated aptamers were studied in the construction of label-free impedimetric sensors. The results showed that the initial modification density of amino aptamers was higher than that of thiol aptamers. Aptamers can recognize and bind OTA to generate electrical signals. The higher the density of aptamer modification was, the better the electric signals were. If only considering the initial modification density, amino aptamers were more suitable for the preparation of aptasensors than thiolated aptamers. However, the modification density of the amino aptamer decreased with the prolonged immersion time in 1 mM HCl solution, which suggests that the stability of this sensor was poor. However, the thiolated aptamer maintained relatively constant density and could be reused. Thus, the thiolated aptasensor had a wide range and good reproducibility and stability for the determination of ochratoxin A (OTA). In addition, this study proved that gold nanoparticles play an important role in signal amplification by increasing the effective gold surface to fix more aptamers in the process of sensor preparation.


Asunto(s)
Aptámeros de Nucleótidos/química , Aptámeros de Nucleótidos/metabolismo , Técnicas Biosensibles/métodos , Impedancia Eléctrica , Electroquímica , Electrodos , Oro/química , Nanopartículas del Metal/química , Ocratoxinas/análisis , Reproducibilidad de los Resultados
9.
Sci Total Environ ; 753: 141653, 2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-32896735

RESUMEN

The source apportionment of pharmaceuticals and personal care products (PPCPs) in the water environment based on indicators (i-PPCPs) requires a comprehensive characterization of various emission sources using reliable analytical methods for a wide spectrum of PPCPs. In this study, a robust and sensitive method based on solid phase extraction (SPE) and ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for analyzing 70 PPCPs belonging to 17 therapeutic classes in landfill leachates and livestock wastewaters was developed. The SPE cartridges, sample pH, elution solvents and chelating agent additions were optimized, and acceptable recoveries (60- 130% for 67 target compounds), low method quantification limits (landfill leachate: 3- 1309 ng/L; livestock wastewater: 3- 686 ng/L) and high precisions (repeatability: 0- 20% for over 99% injections; reproducibility: 0- 20% for over 90% injections) were obtained. Using the optimized analytical method to characterize PPCPs in the typical landfill leachate and livestock wastewater in Yangtze River Delta, China, we found anthelmintics, which were first reported in landfill leachates globally, exhibited the highest concentration (albendazole, maximum concentration of 61.6 µg/L), and therefore proposed albendazole as one of the promising i-PPCP candidates in landfill leachates. In livestock wastewaters, antibiotics lincomycin was the most abundant PPCP (maximum concentration: 735 µg/L) and identified as an i-PPCP candidate for livestock-originated contamination. In addition, 15 non-antibiotic PPCPs were first investigated in the livestock wastewater in China and some non-steroidal anti-inflammatory drugs, acetaminophen, diclofenac and naproxen, were detected at similar concentration level (1.16- 91.1 µg/L) to antibiotics, highlighting the necessity to include representative non-antibiotic PPCPs in the studies of emerging contaminants in livestock wastewaters. The developed method provides a tool to comprehensively investigate PPCPs in high-strength wastewater, and the preliminary findings in the characterization of typical landfill leachates and livestock wastewaters are helpful to select i-PPCPs for the source apportionment of PPCPs in Yangtze River Delta, China.


Asunto(s)
Cosméticos , Preparaciones Farmacéuticas , Contaminantes Químicos del Agua , Animales , China , Cosméticos/análisis , Ganado , Reproducibilidad de los Resultados , Ríos , Espectrometría de Masas en Tándem , Aguas Residuales , Contaminantes Químicos del Agua/análisis
10.
Food Chem ; 336: 127710, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32763739

RESUMEN

Conventional gold nanoparticle-based lateral flow immunoassay (LFIA) usually suffers a huge challenge in measuring target concentration in food matrices with dark color because of its poor resistance to the background matrix and color interference. To address this issue, we first report a novel bifunctional magneto-gold nanohybrid (MGNH) for the simultaneous magnetic separation and colorimetric target sensing by integrating MGNHs into LFIA. Under optimum conditions, an ultrasensitive detection of ochratoxin A (OTA) in grape juice was achieved with a limit of detection at 0.094 ng mL-1. The average recoveries of this MGNH-LFIA ranged from 92.31% to 108.97% with a coefficient of variation of below 12%. The excellent selectivity of our MGNH-LFIA against OTA was demonstrated. Besides, our MGNH-LFIA is comparable to liquid chromatography coupled with mass spectrometry in terms of accuracy, reproducibility, and practicability. The designed MGNH-LFIA platform is readily extended for improving other small molecule detection in food samples.


Asunto(s)
Contaminación de Alimentos/análisis , Jugos de Frutas y Vegetales/análisis , Inmunoensayo/métodos , Nanopartículas del Metal/química , Ocratoxinas/análisis , Análisis de los Alimentos/instrumentación , Análisis de los Alimentos/métodos , Oro/química , Inmunoensayo/instrumentación , Límite de Detección , Fenómenos Magnéticos , Ocratoxinas/inmunología , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Espectrofotometría Ultravioleta , Vitis/química
11.
Food Chem ; 336: 127713, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32768909

RESUMEN

The presence of multiple mycotoxins in the agricultural products poses a serious threat to the health of humans and animals. Citrinin (CIT) causes slow growth in animals and damages the kidney function. Zearalenone (ZEN) causes chronic poisoning, abnormal functioning and even death in animals. Herein, a dual fluorescent immunochromatographic assay (DF-ICA) based on europium nanoparticles (EuNPs) was developed for the simultaneous detection of CIT and ZEN in the corn samples. After optimization, the limits of detection (LODs), IC50 and average recoveries for the simultaneous determination of CIT and ZEN were 0.06 and 0.11 ng/mL, 0.35 and 0.76 ng/mL, from 86.3% to 111.6% and from 86.6% to 114.4%, respectively. Moreover, the DF-ICA was validated by high performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained. In brief, this work demonstrates the feasibility of DF-ICA for simultaneous monitoring of CIT and ZEN in the corn samples.


Asunto(s)
Citrinina/análisis , Contaminación de Alimentos/análisis , Inmunoensayo/métodos , Zea mays/química , Zearalenona/análisis , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/inmunología , Cromatografía Líquida de Alta Presión , Citrinina/inmunología , Europio/química , Fluorescencia , Análisis de los Alimentos/instrumentación , Análisis de los Alimentos/métodos , Humanos , Límite de Detección , Nanopartículas del Metal/química , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Zearalenona/inmunología
12.
Food Chem ; 336: 127675, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32795781

RESUMEN

The present study explores the biosorption potential of Pleurotus ostreatus immobilized magnetic iron oxide nanoparticles for solid-phase extractions of Ni(II) and Pb(II) ions from the water and food samples. It was characterized using FTIR, FE-SEM/EDX before and after analyte ions biosorption. Important operational parameters including the effect of initial pH, the flow rate of the sample solution and volume, amount of biomass and support material, interfering ions, best eluent, column reusability were studied. The biosorption capacities of fungus immobilized iron oxide nanoparticles were found as 28.6 and 32.1 mg g-1 for Ni(II) and Pb(II), respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were achieved as 0.019 and 0.062 ng mL-1 for Ni(II), 0.041 and 0.14 ng mL-1 for Pb(II), respectively. The proposed method was validated by applying to certified reference materials and successfully applied for the preconcentrations of Ni(II) and Pb(II) ions from water and food samples by ICP-OES.


Asunto(s)
Plomo/aislamiento & purificación , Nanopartículas/química , Níquel/aislamiento & purificación , Pleurotus/química , Extracción en Fase Sólida/métodos , Contaminantes Químicos del Agua/aislamiento & purificación , Adsorción , Células Inmovilizadas , Compuestos Férricos/química , Análisis de los Alimentos/instrumentación , Análisis de los Alimentos/métodos , Contaminación de Alimentos/análisis , Concentración de Iones de Hidrógeno , Límite de Detección , Fenómenos Magnéticos , Níquel/química , Reproducibilidad de los Resultados , Extracción en Fase Sólida/instrumentación , Contaminantes Químicos del Agua/química
13.
Food Chem ; 336: 127718, 2021 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-32763741

RESUMEN

A novel dual near-infrared fluorescence-based lateral flow immunosensor was developed to determine zearalenone and deoxynivalenol in maize. Two near-infrared dyes with distinct fluorescence characteristics were utilized to separately label the anti-zearalenone and anti-deoxynivalenol antibodies as detection reagents. The capture antigens zearalenone-BSA and deoxynivalenol-BSA were mixed and immobilized on the same test line of nitrocellulose membrane. This assay format facilitates simultaneous detection of the two mycotoxins on a single test line. After optimizing experimental parameters, the limits of detection for zearalenone and deoxynivalenol were as low as 0.55 µg/kg and 3.8 µg/kg in maize, respectively. The spiking experiment yielded recovery ratios ranging from 81.7% to 107.3% with coefficients of variation less than 14% demonstrating high assay accuracy and precision. Moreover, the actual sample analysis produced consistent results between this method and instrumental method. Therefore, the developed immunosensor can serve as an accurate and efficient approach for monitoring mycotoxins in agricultural products.


Asunto(s)
Inmunoensayo/métodos , Tricotecenos/análisis , Zea mays/química , Zearalenona/análisis , Animales , Anticuerpos/inmunología , Bovinos , Colorantes Fluorescentes/química , Límite de Detección , Micotoxinas/análisis , Micotoxinas/inmunología , Reproducibilidad de los Resultados , Albúmina Sérica Bovina/química , Espectroscopía Infrarroja Corta , Tricotecenos/inmunología , Zea mays/metabolismo , Zearalenona/inmunología
14.
Zhongguo Zhong Yao Za Zhi ; 45(18): 4404-4410, 2020 Sep.
Artículo en Chino | MEDLINE | ID: mdl-33164369

RESUMEN

The chemical fingerprints of American ginseng were established by high performance liquid chromatography(HPLC) coupled with similarity evaluation system for chromatographic fingerprint of traditionanl Chinese medicine. The results were analyzed with use of stoichiometry methods(cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis), and meanwhile, a preliminary study on the antioxidant and anti-proliferation activity of colorectal cancer cells was conducted. By comparing the fingerprints of American ginseng before and after processing, the contents of five components in the eight ginseno-sides quantified in this paper increased, including ginsenoside Rc, Rg_2, Rb_2, Rb_3 and Rd, respectively, and a new component was produced after steaming. The activity study showed that steamed American ginseng had better antioxidant activity and anti-proliferation activity of colorectal cancer cells than raw American ginseng. The research results show that the steaming method of American ginseng used in this experiment has good stability and reproducibility, and the steaming of American ginseng produces similar changes as artificial red ginseng, which provides a certain reference for expanding the application range of American ginseng.


Asunto(s)
Ginsenósidos , Panax , Cromatografía Líquida de Alta Presión , Ginsenósidos/análisis , Reproducibilidad de los Resultados , Vapor
15.
Zhongguo Zhong Yao Za Zhi ; 45(18): 4466-4471, 2020 Sep.
Artículo en Chino | MEDLINE | ID: mdl-33164377

RESUMEN

The present study aims to establish a method for determination of salidroside in plasma and tissues of mice, and to study the pharmacokinetics and distribution characteristics of salidroside in mice. After intragastrically administered of salidroside at doses of 12.30, 36.90 and 73.80 mg·kg~(-1) to mice, plasma and tissue samples were collected, and the concentration of salidroside in each sample was measured by UPLC-MS/MS to study plasma drug parameters and tissue distribution of salidroside. Salidroside showed a good linear relationship in the plasma and tissues of mice in the concentration range of 43.40-5 556 ng·mL~(-1). The intraday and interday precision are less than 15%, and the accuracy is between 79.50% and 98.20%. The tissue distribution study found that salidroside had higher plasma concentration, and the plasma concentration reached the maximum at 0.5 h. After 6 h, no salidroside was detected in the plasma, indicating that salidroside has good solubility and absorption fast, clearance is also fast. In tissues, the concentration of liver and kidney tissues is higher, indicating that salidroside liver and kidney are the main metabolic and excretory organs. The established method is easy to operate and has good precision. It is suitable for salidroside in mice. Pharmacokinetics and tissue distribution studies provide a reference for the mechanism of action and drug development of salidroside.


Asunto(s)
Glucósidos , Espectrometría de Masas en Tándem , Animales , Cromatografía Líquida de Alta Presión , Cromatografía Liquida , Ratones , Fenoles , Reproducibilidad de los Resultados , Distribución Tisular
16.
Zhongguo Zhong Yao Za Zhi ; 45(17): 4221-4229, 2020 Sep.
Artículo en Chino | MEDLINE | ID: mdl-33164407

RESUMEN

To improve and perfect the quality standards and propose recommendations for the revision of quality standards for Andrographis Herba and its processed slices in Chinese Pharmacopoeia(ChP)(2020 edition) based on the problems and limitations in ChP(2015 edition). TLC identification method with andrographolide and control herbs as references was established using silica gel G thin layer plate, with chloroform-methylbenzene-methanol(8∶1∶1) as developing solvent, and 10% sulfuric acid ethanol solution as colour-developing agent. This method has good reproducibility, strong specificity and high sensitivity. As compared with the original method in ChP 2015, this method has better development effect and clearer spots. Based on the previous research, a quantitative analysis of multi-components by single-marker(QAMS) method with andrographolide as the internal reference substance was developed to simultaneously determine the contents of 4 diterpene lactones: andrographolide(S), neoandrographolide(A), 14-deoxyandrographolide(B), and dehydroandrographolide(C). The relative correction factors of f_(A/S), f_(B/S), and f_(C/S) were determined as 1.12, 0.79, and 0.63, respectively. The relative retention time of t_(A/S), t_(B/S), and t_(C/S) was 1.95, 2.18, and 2.25, respectively. According to the content determination results in 46 batches of crude drugs and 38 batches of processed slices, it was stipulated that the total contents of 4 diterpene lactones should not be less than 1.5% and 1.2% in crude drugs and processed slices, respectively. As compared with the original method in ChP 2015, the present QAMS method could not only reduce the detection cost and improve the efficiency, but also can be used to evaluate the quality of Andrographis Herba and its processed slices more comprehensively and objectively. Diterpene lactones are generally recognized as the effective components in Andrographis Herba, and their contents in leaves were much higher than those in stems. However, almost all of the current commercial processed slices are processed from stems, so their quality is gene-rally poor and the efficacy is hard to be guaranteed. Therefore, the weight percentage of leaves should be added into the inspection items of the processed slices and it should not be less than 25%.


Asunto(s)
Andrographis , Medicamentos Herbarios Chinos , Cromatografía Líquida de Alta Presión , Diterpenos/análisis , Reproducibilidad de los Resultados
17.
Zhongguo Zhong Yao Za Zhi ; 45(19): 4719-4724, 2020 Oct.
Artículo en Chino | MEDLINE | ID: mdl-33164438

RESUMEN

To investigate the effect of salidroside on the proteomics of erythrocyte membrane in high altitude erythrocytosis(HAPC) rats, in order to explore the mechanism of salidroside in improving HAPC based on the proteomics analysis. First, HPAC rat models were established, and 16 rats were randomly divided into HAPC model group and salidroside(100 mg·kg~(-1)) treatment group(8 rats per group). Saline was administered to the HAPC model group, while salidroside treatment group was given 100 mg·kg~(-1) salidroside once a day. After continuous oral administration with salidroside for 40 days(once a day), blood was collected from the femoral artery to obtain total red blood cell membrane proteins. Two-dimensional electrophoresis was used to separate total proteins. The two-dimensional electrophoresis of erythrocyte membrane proteins was analyzed before and after salidroside intervention, and the proteins with significant differences were identified by mass spectrometry. Finally, biological functions were analyzed using bioinformatics. A two-dimensional electrophoresis method was used to establish a protein expression profile with a high resolution and reproducibility of erythrocyte membranes in HAPC rats. Salidroside treatment significantly changed 18 protein spots in the 2-DE map of erythrocyte membranes, of which 13 proteins were up-regulated and 5 proteins were down-regulated. Eight differential proteins were successfully identified by mass spectrometry. Moreover, bioinformatics analysis found that these differential proteins were involved in such biological processes as oxidative stress, redox, and peroxisome pathway, which are mainly associated with peroxisome and MAPK signaling pathways. Therefore, salidroside could significantly change the expressions of erythrocyte membrane proteins in HAPC rats. Eight differential proteins were identified by a proteomic-based approach. The differential proteins were involved in such biological processes as oxidative stress, redox, peroxisome pathway.


Asunto(s)
Policitemia , Altitud , Animales , Eritrocitos , Glucósidos , Fenoles , Proteómica , Ratas , Reproducibilidad de los Resultados
18.
Kyobu Geka ; 73(10): 770-774, 2020 Sep.
Artículo en Japonés | MEDLINE | ID: mdl-33130763

RESUMEN

Liver cirrhosis is a major risk factor in patients requiring cardiac surgery. Although current evidence is limited to reports coming mostly from small case series, it is clear that the surgical risk increases with the severity of the liver disease. Hemodynamic instability caused by hyperdynamic circulation, systemic fluid retention, infection, and bleeding is frequently observed postoperatively in severely cirrhotic patients. Preoperative optimization, including correction of coagulopathy and poor nutrition, is therefore crucial for minimizing the predictive postoperative complications in those patients. Postoperative management should focus on bleeding and infection control, body fluid management, adequate nutrition, and hemodynamics, particularly hepatic circulation. Multiple studies have shown that patients who are diagnosed as Child-Pugh class B or C liver cirrhosis have a high surgical mortality rate, with most reports suggesting class C as inoperable. Recently, the model for end-stage liver disease( MELD) score has been gaining attention for its reliability in identifying patients at high risk for open heart surgery. Off-pump surgery may be beneficial in improving the surgical outcomes, but the evidence is weak and further studies are required. A thorough preoperative evaluation is thus mandatory in cirrhotic patients scheduled for cardiac surgery, with a particular attention to the risks and benefits of performing the surgery itself.


Asunto(s)
Procedimientos Quirúrgicos Cardíacos , Niño , Humanos , Cirrosis Hepática/complicaciones , Reproducibilidad de los Resultados , Estudios Retrospectivos , Factores de Riesgo , Índice de Severidad de la Enfermedad
19.
Vestn Otorinolaringol ; 85(5): 44-50, 2020.
Artículo en Ruso | MEDLINE | ID: mdl-33140933

RESUMEN

TO STUDY: The effect of the elemental composition of bone tissue on the destruction of the structures of the temporal bone in chronic suppurative otitis media. PATIENTS AND METHODS: The study included 331 patients aged 16-75 years with a verified diagnosis of chronic purulent otitis media, subject to surgical treatment. Depending on the diagnosis and method of surgical treatment, patients are divided into groups: patients with tubotympanic otitis media and epitympanic-antral otitis media after reconstructive sanitation otosurgery in comparison with the control group. The elemental composition of the bone tissue of the cavities of the middle ear obtained intraoperatively was evaluated by x-ray fluorescence spectroscopy. Elements in the bone tissue of the cavities of the middle ear obtained intraoperatively were evaluated by spectrophotometric method. The data are processed by descriptive statistics methods and are presented in the form of a median and a range between quartiles with an estimate of the reliability of the intergroup differences by the Mann-Whitney U-criterion. RESULTS: A comparison of the indicators characterizing the elemental composition of the temporal bone tissue in patients with forms of chronic otitis media demonstrates the progression of the severity of pathological bone remodeling according to various options. In the dynamics of observation in patients with morphological signs of purulent destruction of the structures of the temporal bone, not only the most profound imbalance in the levels of calcium and phosphorus was revealed, but also the limitation of the levels of silicon and sulfur in a progressive and recurrent course at different periods of the disease. CONCLUSION: X-ray fluorescence spectroscopy allows an elemental chemical analysis of bone remodeling according to the pathological type and reliably differentiate purulent diseases of the temporal bone. The results are promising from the point of view of developing new prognostic approaches in otosurgery in the management of patients with chronic purulent otitis media, involving the combined use of morphological research methods with microelement analysis of the temporal bone bone tissue.


Asunto(s)
Otitis Media Supurativa , Otitis Media , Adolescente , Adulto , Anciano , Enfermedad Crónica , Oído Medio/diagnóstico por imagen , Oído Medio/cirugía , Humanos , Persona de Mediana Edad , Otitis Media Supurativa/diagnóstico , Otitis Media Supurativa/cirugía , Reproducibilidad de los Resultados , Hueso Temporal/diagnóstico por imagen , Hueso Temporal/cirugía , Adulto Joven
20.
Sci Rep ; 10(1): 18899, 2020 11 03.
Artículo en Inglés | MEDLINE | ID: mdl-33144632

RESUMEN

Severe acute respiratory coronavirus 2 (SARS-CoV-2) testing reagents are expected to become scarce worldwide. However, little is known regarding whether pooling of samples accurately detects SARS-CoV-2. To validate the feasibility of pooling samples, serial dilution analysis and spike-in experiments were conducted using synthetic DNA and nucleic acids extracted from SARS-CoV-2-positive and -negative patients. Furthermore, we studied 1000 individuals, 667 of whom were "healthy" individuals (195 healthcare workers and 472 hospitalized patients with disorders other than COVID-19 infection), and 333 infection-suspected patients with cough and fever. Serial dilution analysis showed a limit of detection of around 10-100 viral genome copies according to the protocol of the National Institute of Infectious Diseases, Japan. Spike-in experiments demonstrated that RT-qPCR detected positive signals in pooled samples with SARS-CoV-2-negative and -positive patients at 5-, 10-, 20-fold dilutions. By screening with this pooling strategy, by the end of April 2020 there were 12 SARS-CoV-2-positive patients in 333 infection-suspected patients (3.6%) and zero in 667 "healthy" controls. We obtained these results with a total of 538 runs using the pooling strategy, compared with 1000 standard runs. In a prospective study, we successfully detected SARS-CoV-2 using 10- to 20-fold diluted samples of nasopharyngeal swabs from eighteen COVID-19 patients with wide ranges of viral load. Pooling sample is feasible for conserving test reagents and detecting SARS-CoV-2 in clinical settings. This strategy will help us to research the prevalence infected individuals and provide infected-status information to prevent the spread of the virus and nosocomial transmission.


Asunto(s)
Técnicas de Laboratorio Clínico/métodos , Tamizaje Masivo/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Betacoronavirus/genética , Betacoronavirus/patogenicidad , Técnicas de Laboratorio Clínico/normas , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/epidemiología , Humanos , Límite de Detección , Tamizaje Masivo/normas , Reproducibilidad de los Resultados , Mucosa Respiratoria/virología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/normas
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