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1.
Food Chem ; 367: 130669, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34365252

RESUMEN

Analysis of digital images by smartphone was used for copper quantification in sugarcane spirit (cachaça) samples through the formation of blue complex between copper and cuprizone. An experimental design was carried out to evaluate the best complexation reaction conditions. Moreover, different image acquisition procedures (external camera coupled to a smartphone or the smartphone camera) with different regions of interest sizes, distances in image acquisition, and concentration ranges of the calibration curve and the influence of processing the curve in univariate and multivariate modes, by PLS, were evaluated. The results obtained in three real samples and two spikes were compared with those of UV-Vis spectrophotometry, used as a reference method, and they show the potential of the proposed method for the accurate determination of copper. When compared to traditional techniques, the proposed method has the advantages of portability and low cost in addition to requiring a smaller amount of reagents.


Asunto(s)
Cobre , Saccharum , Grano Comestible , Proyectos de Investigación , Teléfono Inteligente
2.
Food Chem ; 367: 130657, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34388631

RESUMEN

Non-centrifugal cane sugar (NCS) is the second most important Colombian agribusiness in social importance. However, the sugar cane industry is facing some challenges caused by the controversial nutritional and safety attributes of NCS. Some Colombian NCS producers employ natural mucilages as clarifiers; but the uncontrolled application of these components has caused a risk of extinction in the mucilage source plants. Other producers employ acrylamide as a clarifier. Health consequences have generated concerns from the consumers and demanded control from the food authorities. Efforts are being made to develop a standard manufacturing methodology to increase NCS productivity and improve its quality, hygiene, and storability. The application of better clarifiers, which provide the best clarifying activity and minimize the toxicity while conserving NCS's natural attributes, is one of the outstanding challenges as well. This study is a proposal which looks for sustainable, natural, nontoxic, and economical clarifiers for the Colombian NCS producers.


Asunto(s)
Saccharum , Azúcares , Acrilamida , Polisacáridos
3.
Food Chem ; 368: 130731, 2022 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-34404003

RESUMEN

Rapadura is an artisanal candy obtained from concentrated sugarcane juice. In this study, a differentiation between South American rapadura producers has been tried using a Kurtosis-based projection pursuit analysis (kPPA) concerning essential minerals, acrylamide, moisture contents, pH, and color. These parameters revealed significant inter- and intra-country differences. Based on the employed measurements, a multivariate exploration with kPPA extracted information from rapadura even though it is a very artisanal product and was effective in separating classes, especially Brazilian and Ecuadorian rapadura, where principal component analysis failed. Moreover, ellipse confidence regions showed significant differences between non-organic and organic rapadura from Colombia and Peru in granulated form. From a chemometric point of view, the application of kPPA can be used in cases when other metrics (as based on the variance) fail and can be useful in the exploratory analysis of complex multivariate chemical data.


Asunto(s)
Acrilamida , Saccharum , Brasil , Minerales , Análisis de Componente Principal
4.
Environ Pollut ; 292(Pt A): 118267, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34601036

RESUMEN

Distillery sludge is a major source of aquatic pollution, but little is known about their microbial community and their association with the organic and metal pollutants. Sugarcane molasses-based distillery is an important industry in India, although the waste is usually treated prior to disposal, the treatment is often inadequate. The adverse effects of the organic and metal pollutants in sugarcane molasses-based distillery sludge on the microbial biodiversity and abundance in the disposal site have not been elucidated. This study aims to address this gap of knowledge. Samples were collected from the discharge point, 1 and 2 km downstream (D1, D2, and D3, respectively) of a sugarcane distillery in Uttar Pradesh, India, and their physico-chemical properties characterised. Using QIIME, taxonomic assignment for the V3 and V4 hypervariable regions of 16 S rRNA was performed. The phyla Proteobacteria (28-39%), Firmicutes (20-28%), Bacteriodetes (9-10%), Actinobacteria (5-10%), Tenericutes (1-9%) and Patescibacteria (2%) were the predominant bacteria in all three sites. Euryechaeota, were detected in sites D1 and D2 (1-2%) but absent in D3. Spirochaetes (5%), Sinergistetes (2%) and Cloacimonetes (1%) were only detected in samples from site D1. Shannon, Simpson, Chao1, and Observed-species indices indicated that site D1 (10.18, 0.0013, 36706.55 and 45653.84, respectively) has higher bacterial diversity and richness than D2 (6.66, 0.0001, 25987.71 and 49655.89, respectively) and D3 (8.31, 0.002, 30345.53 and 30654.88, respectively), suggesting the organic and metal pollutants provided the stressors to favour the survival of microbial community that can biodegrade and detoxify them in the distillery sludge. This study confirmed that the treatment of the distillery waste was not sufficiently effective and provided new metagenomic information on its impact on the surrounding microbial community. It also offered new insights into potential bioremediation candidates.


Asunto(s)
Contaminantes Ambientales , Microbiota , Saccharum , Melaza , Aguas del Alcantarillado , Eliminación de Residuos Líquidos , Aguas Residuales
5.
J Environ Manage ; 301: 113792, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34607137

RESUMEN

Jaggery is a kind of unrefined non-centrifugal sugar (NCS) used mainly in Asia, Africa, Latin America, and the Caribbean. Traditionally, jaggery is produced by concentrating sugarcane juice in open pans with the help of bagasse combustion. However, due to thermal energy loss with flue gases and an unscientific approach in plant construction, jaggery plants have a poor thermal efficiency of less than 25%, poor emission characteristics, and a high bagasse consumption rate. Advanced jaggery-making techniques use solar energy and heat pumps for jaggery production. However, these techniques are in the early stage of development, and the literature indicates that these techniques should be used in conjuction with traditional ones to improve the performance of jaggery making plants. This literature review describes advances in jaggery-making methods, critically analyzed them, and provides a qualitative comparison of these methods. Further, gaps in the existing literature are identified and reported for future research direction. In addition, efforts have been made to quantify and estimate the emissions reduction and bagasse consumption potentials from the traditional jaggery industry to make this rural industry a sustainable and profitable business for rural entrepreneurs. The comparison with the recently developed clean combustion device exhibits that the harmful emissions from the jaggery industry could be reduced drastically viz. 95%-98% of PM2.5; 92%-95% of CO, and 52-60% of CO2, while saving more than 35% of bagasse consumption. Implemented at a national scale, it may reduce nearly 3% of all harmful emissions in the country, which is equally applicable elsewhere.


Asunto(s)
Extractos Vegetales , Saccharum , Gases , Calor
6.
Sci Total Environ ; 803: 150019, 2022 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-34500267

RESUMEN

Reducing nitrogen (N) losses from cropping systems to aquatic ecosystems is a global priority. In Australia, N losses from sugarcane production in catchments adjacent to the Great Barrier Reef (GBR) are threatening the health of this World Heritage-listed coral reef ecosystem. N losses from sugarcane can be reduced by improving fertiliser management. However, little is known about the contribution of organic sources of N, such as mill mud. We used more than 10 years of data from two of the main Australian sugarcane regions, a high (Wet Tropics) and moderate (Mackay Whitsundays) rainfall area, to calibrate and validate a model to predict dissolved inorganic nitrogen (DIN) losses in runoff from both inorganic and organic fertilisers. DIN losses in runoff were well simulated (RMSE = 0.37 and 2.0 kg N ha-1 for the Wet Tropics and Mackay Whitsunday regions, respectively). Long-term simulations of rate and fertiliser deductions to account for N from organic sources showed that adopting best management practices for organic fertiliser (applying ≤50 wet t ha-1 mill mud) can significantly reduce DIN in runoff losses compared with applications of 150 wet t ha-1. Simulations of typical farmer practices in relation to fallow management (bare fallow vs. legume fallow) and organic fertiliser placement (buried in a fallow but surface applied to a green cane trash blanket in ratoons) showed that inorganic fertiliser rates need to be adjusted to account for N inputs from both mill mud and legume crops. Rates of application of organic N had a larger impact on DIN runoff losses than placement or timing of application. This work presents a DIN in runoff modelling algorithm that can be coupled with nitrogen models readily available in agricultural models to assess the impact of nutrient management on the quality of water leaving agricultural systems.


Asunto(s)
Nitrógeno , Saccharum , Agricultura , Algoritmos , Australia , Ecosistema , Monitoreo del Ambiente , Nitrógeno/análisis , Nutrientes , Fósforo/análisis
7.
J Sci Food Agric ; 102(1): 312-321, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-34096072

RESUMEN

BACKGROUND: Sugarcane straw is an available but largely ignored lignocellulosic biomass to obtain cellulose nanocrystals (CNCs) with highly crystalline, tunable surface chemistries and a wide-ranging adaptability. Herein, we utilized sugarcane straw to obtain pure cellulose via purification processes, followed by subsequent preparation of CNCs via sulfuric acid hydrolysis. The properties of the purified fibers and obtained CNCs were assessed by their composition, morphology, chemical structure, crystallinity and thermal stability. RESULTS: After the purification process, alkali-treated fibers (ATFs) contained 886.33 ± 1.25 g kg-1 cellulose, and its morphological analysis revealed a smooth and slender fibrous structure. The CNCs obtained by treatment with 64 wt% sulfuric acid at 45 °C for 60 min were isolated in a yield of 21.8%, with a diameter and length of 6 to 10 nm and 160 to 200 nm, respectively. Moreover, crystallinity index of these CNCs reached 62.66%, and thermal stability underwent a two-step degradation. Short-term ultrasonication after hydrolysis was employed to enhance isolation of the CNC particles and improve the anionic charge with higher value -38.00 mV. CONCLUSION: Overall, isolation and characterization results indicated the potential for CNCs preparation using sugarcane straw, in addition to offering a fundamental understanding of this material and indicating potential applications. © 2021 Society of Chemical Industry.


Asunto(s)
Celulosa/química , Celulosa/aislamiento & purificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Saccharum/química , Residuos/análisis , Hidrólisis , Nanopartículas/química , Tallos de la Planta/química , Ácidos Sulfúricos
8.
Environ Monit Assess ; 193(12): 789, 2021 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-34757510

RESUMEN

Rainfall is generally partitioned into throughfall, stemflow, and interception in ecosystems. Stemflow variability can affect the hydrology, ecology, and soil chemistry patterns. However, the influence of canopy structure and rainfall characteristics on stemflow production in sugarcane plantations which are important for renewable energy production remain poorly understood. By using funnels attached to the sugarcane stems, the present study determined the stemflow amount during the period of sugarcane growth and its relationship with plant development. Approximately, 14% of gross rainfall reached the soil as stemflow, and the funneling ratios was 60. In general, it was observed a positive relationship between stemflow rates with both leaf area index and plant height. This was attributed to an increasing number of acute branching angles of the sugarcane leaves as well as high stem tillering and density. However, at the end of growth cycle, stemflow rate was lower than in previous periods which can be attributed to changes in sugarcane canopy such as stems inclination and lodging, reducing the effectiveness of water conveyance along the stem. Our study showed the need to include stemflow to better understand the hydrology of sugarcane plantations.


Asunto(s)
Lluvia , Saccharum , Ecosistema , Monitoreo del Ambiente , Suelo , Árboles
9.
Aquat Toxicol ; 241: 106017, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34773901

RESUMEN

With the growing use of agrochemicals in Brazil, there is also a growing need for more realistic toxicity assessments that aid in understanding the potential risks of environmental-realistic agrochemical (mixture) exposures in the natural ecosystems. The aim of the present study was therefore to evaluate the lethal and sublethal effects of environmental realistic (single and mixture) concentrations of the pesticides DMA® 806 BR (active ingredient - a.i. 2,4-D) and Regent® 800 WG (a.i. fipronil) and sugarcane vinasse to the Neotropical cladoceran Ceriodaphnia silvestrii. This evaluation was carried out through lethal (survival), sublethal (reproduction and intrinsic rates of population increase - r) and post-exposure (feeding rate and also reproduction) tests conducted in situ and with water from mesocosms contaminated with the recommended doses of these compounds. The results showed high acute toxicity for treatments containing fipronil and vinasse when acting in isolation, with survival rates only returning to control values on the last sampling day (75 days post application). Reproduction of surviving cladocerans was reduced in all treatments until the end of the experiment and were potentiated effect in the mixture of the three test compounds. The intrinsic rates of population increase were reduced in all treatments except the single 2,4-D treatment. Post-exposure feeding rate and reproduction, however, were not impaired under the conditions analyzed. The results show the high toxicity of recommended doses of fipronil and vinasse (and especially their mixture) and the importance of evaluating the risks of agrochemical mixtures at environmental-realistic concentrations.


Asunto(s)
Cladóceros , Plaguicidas , Saccharum , Contaminantes Químicos del Agua , Animales , Ecosistema , Contaminantes Químicos del Agua/toxicidad
10.
Environ Monit Assess ; 193(12): 828, 2021 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-34796405

RESUMEN

Within Pacific Small Island Developing States (Pacific SIDS), the ridge-to-reef (R2R) approach has emerged as a framework for monitoring river connectivity between terrestrial and marine ecosystems. The study measured water quality, including pH, over 88.40 km of the Ba River in Fiji. The sampling design focused on measuring spatio-temporal variability in pH throughout the sugarcane season with three rapid sampling periods (RSP1, 2 & 3) along the Ba River, together with continuous measurement of temperature and pH using stationary data loggers at two locations upstream and downstream of the sugar mill. Spatial variability in pH and water quality was characterised before (RSP1 and RSP2) and during (RSP3) the sugarcane season. Mean pH measured before the sugarcane crushing season for RSP1 and RSP2 were 8.16 (± 0.49) and 8.20 (± 0.61) respectively. During the sugarcane crushing season (RSP3), mean pH declined by 3.06 units to 6.94 within 42 m downstream of the sugar mill (P ≤ 0.001). The 3.06 unit decline in pH for RSP3 exceeded both the mean diurnal variation in pH of 0.39 and mean seasonal variation in pH of 2.01. This decline in pH could be a potential source of acidification to downstream coastal ecosystems with implications for coral reefs, biodiversity and fishery livelihoods.


Asunto(s)
Antozoos , Saccharum , Animales , Arrecifes de Coral , Ecosistema , Monitoreo del Ambiente , Fiji , Concentración de Iones de Hidrógeno , Estaciones del Año
11.
BMC Genomics ; 22(1): 767, 2021 Oct 27.
Artículo en Inglés | MEDLINE | ID: mdl-34706643

RESUMEN

BACKGROUND: SQUAMOSA promoter binding proteins (SBPs) genes encode a family of plant-specific transcription factors involved in various growth and development processes, including flower and fruit development, leaf initiation, phase transition, and embryonic development. The SBP gene family has been identified and characterized in many species, but no systematic analysis of the SBP gene family has been carried out in sugarcane. RESULTS: In the present study, a total of 50 sequences for 30 SBP genes were identified by the genome-wide analysis and designated SsSBP1 to SsSBP30 based on their chromosomal distribution. According to the phylogenetic tree, gene structure and motif features, the SsSBP genes were classified into eight groups (I to VIII). By synteny analysis, 27 homologous gene pairs existed in SsSBP genes, and 37 orthologous gene pairs between sugarcane and sorghum were found. Expression analysis in different tissues, including vegetative and reproductive organs, showed differential expression patterns of SsSBP genes, indicating their functional diversity in the various developmental processes. Additionally, 22 SsSBP genes were predicted as the potential targets of miR156. The differential expression pattern of miR156 exhibited a negative correlation of transcription levels between miR156 and the SsSBP gene in different tissues. CONCLUSIONS: The sugarcane genome possesses 30 SsSBP genes, and they shared similar gene structures and motif features in their subfamily. Based on the transcriptional and qRT-PCR analysis, most SsSBP genes were found to regulate the leaf initial and female reproductive development. The present study comprehensively and systematically analyzed SBP genes in sugarcane and provided a foundation for further studies on the functional characteristics of SsSBP genes during different development processes.


Asunto(s)
Saccharum , Regulación de la Expresión Génica de las Plantas , Genómica , Familia de Multigenes , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Saccharum/genética , Saccharum/metabolismo
12.
Braz J Biol ; 83: e250879, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34669806

RESUMEN

In the state of São Paulo, the main sugarcane producing region of the world, two species of scale insects have frequently occurred, Aclerda takahashii (Kuwana, 1932) (Hemiptera: Aclerdidae) and Saccharicoccus sacchari (Cockerell, 1895) (Hemiptera: Pseudococcidae). To map the distribution and abundance of these species, 17 sugarcane producing fields, distributed in six mesoregions in São Paulo, were evaluated in August 2017 and, January, February, June and July 2018 during the ripening phase. The study on the seasonality of these species, by the presence or absence of the scale insects during the phenological cycle of the plant, was conducted between August 2017 and July 2018 in two sugarcane producing fields in the municipality of Jaboticabal, São Paulo, Brazil. The presence of S. sacchari was found in all the analyzed locations, and A. takahashii in twelve. Both scale insects showed significant difference of infestation in the node's region of the stems during the ripening phase in one of the studied locations. The aclerdid presented significant difference by infestation in one site during the ripening phase of the plant. The pseudococcid infested a greater number of nodes in the following phases of development; vegetative, grand growth and ripening in both studied areas, but it was in one site during the ripening phase that presented the greatest difference. Although the pink sugarcane mealybug was more abundant than A. takahashii in both studies, there were no patterns of relationships between the numbers of individuals to geographical locations and temperature.


Asunto(s)
Hemípteros , Saccharum , Animales , Brasil , Productos Agrícolas , Humanos , Temperatura
13.
Water Sci Technol ; 84(8): 2028-2039, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34695028

RESUMEN

The vinasse is a residue of ethanol production with the potential for methane production, requiring an allochthonous inoculum. Several microorganisms act in the different phases of anaerobic digestion, and the identification of these microbial communities is essential to optimize the process. The characterization of the microbiota involved in the biodigestion of vinasse was observed in the initial stage (IS), at the peak of methane production (MS) and the end of the process (FS) of the best performance assay by high-throughput sequencing. The highest methane production was 0.78 mmolCH4.gVS.h-1 at 243.7 h in the substrate/inoculum ratio of 1.7, with consumption partial of acetic, propionic and isobutyric acids and an 82% reduction of chemical oxygen demand. High microbial diversity was found. The genera Clostridium, Acinetobacter, Candidatus Cloacamonas, Bacteroides, Syntrophomonas, Kosmotoga, the family Porphyromonadaceae and the class Bacteroidia were the most abundant in the maximum methane production. Methane production was driven by Methanobacterium and Methanosaeta, suggesting the metabolic pathways used were hydrogenotrophic and acetoclastic.


Asunto(s)
Microbiota , Saccharum , Análisis de la Demanda Biológica de Oxígeno , Reactores Biológicos , Metano
14.
Planta ; 254(6): 109, 2021 Oct 27.
Artículo en Inglés | MEDLINE | ID: mdl-34705112

RESUMEN

MAIN CONCLUSION: Differences in cell wall components between two BNF-contrasting sugarcane genotypes might result from genetic variations particular to the genotype and from the efficiency in diazotrophic bacteria association. Sugarcane is a plant of the grass family (Poaceae) that is highly cultivated in Brazil, as an important energy resource. Commercial sugarcane genotypes may be successfully associated with beneficial endophytic nitrogen-fixing bacteria, which can influence several plant metabolic pathways, such as cell division and growth, synthesis of hormones, and defense compounds. In this study, we investigated how diazotrophic bacteria associated with sugarcane plants could be involved in the regulation of cell wall formation pathways. A molecular and structural characterization of the cell wall was compared between two genotypes of sugarcane with contrasting rates of Biological Nitrogen Fixation (BNF): SP70-1143 (high BNF) and Chunee (low BNF). Differentially expressed transcripts were identified in transcriptomes generated from SP70-1143 and Chunee. Expression profiles of cellulose and lignin genes, which were more expressed in SP70-1134, and callose genes, which were more expressed in Chunee, were validated by RT-qPCR and microscopic analysis of cell wall components in tissue sections. A similar expression profile in both BNF-contrasting genotypes was observed in naturally colonized plants and in plants inoculated with G. diazotrophicus. Cell walls of the high BNF genotype have a greater cellulose content, which might contribute to increase biomass. In parallel, callose was concentrated in the vascular tissues of the low BNF genotype and could possibly represent a barrier for an efficient bacterial colonization and dissemination in sugarcane tissues. Our data show a correlation between the gene profiles identified in the BNF-contrasting genotypes and a successful association with endophytic diazotrophic bacteria.


Asunto(s)
Saccharum , Bacterias , Pared Celular/metabolismo , Genotipo , Fijación del Nitrógeno , Saccharum/genética
15.
Front Public Health ; 9: 713711, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34712636

RESUMEN

Background: Chronic kidney disease of non-traditional origin (CKDnt) is an ongoing epidemic that has taken the lives of tens of thousands of people in Mesoamerica, also affecting other tropical geographies. Occupational heat stress, which will increase worldwide as climate change persists, has been identified as a primary trigger of kidney injury and reduced renal function. At Nicaragua's largest sugarcane mill, the water, rest, and shade (WRS) intervention has proven to reduce the risk of heat stress and kidney injury effectively as assessed by the research and policy NGO La Isla Network (LIN) and their academic partners, who have worked with the sugar mill to improve the design of their intervention system. However, discrepancies between intervention design and implementation have been found. This study explores the perceptions of the WRS intervention in the company from the perspective of positions responsible for the workers' environment and heat stress prevention implementation. Methods: A qualitative design was used in the study. Twenty-one key informants of low and middle management, field assistants, and two members from LIN took part in the study. Semi-structured interviews were used to collect the data. Interviews' transcriptions were analyzed using interpretative phenomenological analysis (IPA). Results: Four main themes were developed in the analysis of the data: "A worthwhile struggle," "Culture of care", "Traditional production culture Vs. Culture of care," and "The importance of the formalization of care." Each theme contained sub-themes, all of which were further discussed in the light of organizational psychology. Conclusion and Implications: Discretionary differences resulting in low and middle management prioritizing production over health protection appeared to relate to a fair part of the implementation challenges and indicate that more efforts are needed to align operations' production and health goals. Education enhancement might be necessary, while further focus on health metrics for performance assessment might offer an opportunity to level perceived incentives and value of health and production.


Asunto(s)
Trastornos de Estrés por Calor , Insuficiencia Renal Crónica , Saccharum , Trastornos de Estrés por Calor/prevención & control , Respuesta al Choque Térmico , Humanos , Nicaragua/epidemiología
16.
Appl Microbiol Biotechnol ; 105(23): 8969-8987, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34698899

RESUMEN

This work performed co-AD from the vinasse and filter cake (from 1G ethanol production) and deacetylation liquor (from the pretreatment of sugarcane straw for 2G ethanol production) in a semi-Continuous Stirred Tank Reactor (s-CSTR) aiming to provide optimum operational parameters for continuous CH4 production. Using filter cake as co-substrate may allow the reactor to operate throughout the year, as it is available in the sugarcane off-season, unlike vinasse. A comparison was made from the microbial community of the seed sludge and the reactor sludge when CH4 production stabilized. Lactate, butyrate, and propionate fermentation routes were denoted at the start-up of the s-CSTR, characterizing the acidogenic phase: the oxidation-reduction potential (ORP) values ranged from -800 to -100 mV. Once the methanogenesis was initiated, alkalizing addition was no longer needed as its demand by the microorganisms was supplied by the alkali characteristics of the deacetylation liquor. The gradual increase of the applied organic load rates (OLR) allowed stabilization of the methanogenesis from 3.20 gVS L-1 day-1: the highest CH4 yield (230 mLNCH4 g-1VS) and average organic matter removal efficiency (83% ± 13) was achieved at ORL of 4.16 gVS L-1 day-1. The microbial community changed along with the reactor operation, presenting different metabolic routes mainly due to the used lignocellulosic substrates. Bacteria from the syntrophic acetate oxidation (SAO) process coupled to hydrogenotrophic methanogenesis were predominant (~ 90% Methanoculleus) during the CH4 production stability. The overall results are useful as preliminary drivers in terms of visualizing the co-AD process in a sugarcane biorefinery integrated to scale. KEY POINTS: • Integration of 1G2G sugarcane ethanol biorefinery from co-digestion of its residues. • Biogas production from vinasse, filter cake, and deacetylation liquor in a semi-CSTR. • Lignocellulosic substrates affected the biochemical routes and microbial community. • Biomol confirmed the establishment of the thermophilic community from mesophilic sludge.


Asunto(s)
Saccharum , Anaerobiosis , Biocombustibles , Reactores Biológicos , Digestión , Metano
17.
Physiol Plant ; 173(4): 2276-2290, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34609754

RESUMEN

Increased atmospheric CO2 and consequent increases in temperature are two prominent features of climate change, a major challenge to crops. Here, our objectives were to determine: (1) the responses of sugarcane during the first 90 days of elevated CO2 (ECO2 ) and elevated temperature (ETem), both individually and together, and (2) the genotypic variation of these responses. Eight varieties were grown both in open-top chambers in a factorial combination of ambient/ECO2 concentrations (344-351/777-779 ppm) and ambient/ETem (34.9-35.6/36.6-38.4°C) and in open fields. Significant treatment × variety interaction effects were observed on leaf net photosynthetic rate (An ), stomatal conductance (gs ), transpiration rate (El ), and instantaneous transpiration efficiency (TE ). In most varieties, ECO2 alone did not affect An, but the combination of ECO2 and ETem decreased An . ECO2 decreased gs and El while increasing TE in all varieties. These effects were amplified when ETem was combined with ECO2 . ETem alone had variable effects on An and gs depending on variety, while it increased El and did not affect TE in a majority of varieties. Germination, tillering and stem diameter were not affected by treatments and did not show varietal variation. Leaf water potential, chlorophyll (spad), leaf area, and aboveground dry weight per plant showed varietal variations but were not affected by treatments. The variable responses to ETem and the significant genotypic variation to ECO2 and elevated temperature (ETem) observed in this work, both individually and together, demonstrate a considerable scope to breed sugarcane varieties for a future high-CO2 and warmer climate.


Asunto(s)
Dióxido de Carbono , Saccharum , Fotosíntesis , Hojas de la Planta/genética , Saccharum/genética , Temperatura
18.
Food Res Int ; 149: 110677, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34600679

RESUMEN

Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal microbial composition and short-chain fatty acid production. The unpurified and purified FOS substrates, which were a mixture of 1-kestose, nystose and 1F-fructosylnystose, were supplemented into human faecal culture using a pH-controlled batch fermentation system and significantly increased the Bifidobacterium counts after 5 h fermentation, while Bacteroides/Prevotella counts were highest throughout 24 h fermentation. Meanwhile, Lactobacillus/Enterococcus exhibited a slight increase after 5 h fermentation before reaching a plateau afterwards. The steady Bacteroides/Prevotella growth and increased Bifidobacterium population promoted an increase in the production of short-chain fatty acids acetate (58 ± 2.70 mM), propionate (9.19 ± 5.94 mM) and butyrate (7.15 ± 2.28 mM). These results provide evidence that representative gut microbiota could utilise the enzymatically synthesised FOS to generate short-chain fatty acids as metabolites in pH-controlled conditions, thus FOS from SS are a potential prebiotic ingredient for foods and health drinks.


Asunto(s)
Microbioma Gastrointestinal , Saccharum , Ácidos Grasos Volátiles , Heces , Humanos , Oligosacáridos
19.
Molecules ; 26(19)2021 Sep 28.
Artículo en Inglés | MEDLINE | ID: mdl-34641411

RESUMEN

The method of solvothermal by one-step operation has been performed to synthesize of magnetic amine-functionalized sugarcane bagasse biocomposites (SB-MH). The obtained SB-MH contains 62.34% of Fe, 17.8 mmol/g of amine, and a magnetic property of 19.46 emu/g. The biocomposite surface area increased significantly from 1.617 to 25.789 m2/g after amine functionalization. The optimum condition of SB-MH used for Pb(II) ion removal was achieved at pH 5 for 360 min with adsorption capacity of 203.522 mg/g. The pseudo 2nd order was well-fitted to the model of Pb(II) ion adsorption. Meanwhile, other contaminant parameters number of Chemical Oxygen Demand (COD), Total Suspended Solid (TSS), and dye in wastewater were also remarkably reduced by about 74.4%, 88.0%, and 96.7%, respectively. The reusability of SB-MH with 4th repetitions showed only a slight decrease in performance of 5%. Therefore, the proposed magnetic amine-functionalized sugarcane bagasse biocomposites lead to a very potential adsorbent implemented in high scale due to high surface area, easy separation, stable materials and capability to adsorb contaminants from aqueous solution.


Asunto(s)
Aminas/química , Materiales Biocompatibles/química , Celulosa/química , Magnetismo , Saccharum/química , Aguas Residuales/química , Contaminantes Químicos del Agua/aislamiento & purificación , Concentración de Iones de Hidrógeno , Purificación del Agua/métodos
20.
Anal Chim Acta ; 1177: 338844, 2021 Sep 08.
Artículo en Inglés | MEDLINE | ID: mdl-34482892

RESUMEN

In this work, we describe for the first time the synthesis of a thiocarbazone for the selective determination of Cu2+ in distilled beverages. The method was based on the complexation reaction of Cu2+ with the thiocarbazone, and the colored product was analyzed using a smartphone application. The thiocarbazone reacts with Cu2+ to form a 1:1 (metal:ligand) complex. The Cu2+ complex was characterized by UV, IR and NMR spectral analyses. The proposed reaction yields a yellow color, and therefore, channel B of the RGB system was used in the analysis. After optimizing the reaction conditions, an analytical curve was obtained to determine Cu2+ concentrations ranging between 0.25 and 6.75 mg L-1; the use of 400 µL sample volumes led to a relative standard deviation (n = 5) of 3.2% and a detection limit of 0.18 mg L-1. Recovery experiments were performed with sugar cane spirits, whiskies and tequilas to evaluate the accuracy of the method, and the recovery obtained ranged from 80.5 to 112.2%.


Asunto(s)
Complejos de Coordinación , Saccharum , Bebidas Alcohólicas/análisis , Bebidas , Cobre/análisis , Grano Comestible/química
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