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1.
Public Health ; 190: 62-66, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33360028

RESUMEN

OBJECTIVES: This study aimed to investigate the epidemiological characteristics of syphilis between 2013 and 2018 in the Haidian District of Beijing in China. In addition, this study suggests potential strategies to control the spread of syphilis infection. STUDY DESIGN: Observational study. METHODS: Data were collected from the Infectious Disease Reporting System at medical institutions within the Haidian District of Beijing between 2013 and 2018. Epidemiological methods, combined with Chi-squared test, were used to analyse the distribution of syphilis in terms of time, region, population and development stage. RESULTS: In total, 3048 patients with syphilis were reported in the Haidian District between 2013 and 2018, with an average annual prevalence of 14.26 per 100,000 population. The prevalence of syphilis was higher in rural than urban regions, and the sex ratio of male-to-female was 1.52:1. Patients aged 20-29 years accounted for 22.60% (689/3048) of the total cases. In terms of occupational distributions, the highest proportion (30.61%; 933/3048) was seen in individuals who were homemakers or unemployed. Latent syphilis was present in 66.83% (2037/3048) of patients and accounted for an increased proportion of syphilis cases each year. CONCLUSIONS: Syphilis was epidemic in the Haidian District of Beijing between 2013 and 2018. High prevalence was seen in young and middle-aged males living in urban areas. The prevalence of syphilis has been increasing since 2016; thus, public health policies and intervention strategies need to be strengthened to curb the spread of infection.


Asunto(s)
Población Rural/estadística & datos numéricos , Sífilis/diagnóstico , Sífilis/epidemiología , Treponema pallidum/aislamiento & purificación , Población Urbana/estadística & datos numéricos , Adolescente , Adulto , Distribución por Edad , Anciano , China/epidemiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Vigilancia de la Población , Prevalencia , Distribución por Sexo , Serodiagnóstico de la Sífilis , Adulto Joven
2.
Rev Bras Epidemiol ; 23: e200103, 2020.
Artículo en Portugués, Inglés | MEDLINE | ID: mdl-33053094

RESUMEN

OBJECTIVE: To analyze how syphilis detection rates evolved from 2011 to 2017 according to sex, age and place of residence in the state of São Paulo. METHODS: A historical series was organized with data from the Notification Disease Information System. The acquired syphilis detection rates (ASDR) per 100,000 inhabitants and the acquired syphilis detection rates including pregnant women with syphilis (PASDR) per 100,000 inhabitants were described. For a trend analysis of the rates in the studied period, the Poisson Jointpoint (inflection point) model was performed, and the annual percentage change (APC) per segment and the average annual percentage change (AAPC) were estimated, with respective 95% confidence intervals (95%CI). RESULTS: A total of 205,424 cases of acquired syphilis and syphilis in pregnant women in the period were reported. The ASDR per 100,000 inhabitants ranged from 26.0 to 84.6 between 2011 and 2017 and the PASDR per 100,000 inhabitants ranged from 33.7 to 108.9; the trend was increasing in both, and an inflection point was identified dividing the ASDR and PASDR curve into two periods: 2011 to 2013 and 2013 to 2017: the AAPC found for ASDR was 21.0% (95%CI 15.5 ‒ 26.4) and the PASDR was 21.2% (95%CI 16.4 ‒ 26.1), in the age groups up to 24 years old, there was a significant growth in both sexes. A heterogeneity in the evolution of rates by region of the state was observed between 2011 and 2017. CONCLUSIONS: The increasing trend in acquired syphilis detection rates can be attributed to better adherence to notification and disproportionate involvement of young people.


Asunto(s)
Notificación de Enfermedades/estadística & datos numéricos , Serodiagnóstico de la Sífilis/estadística & datos numéricos , Sífilis/epidemiología , Treponema pallidum/aislamiento & purificación , Adolescente , Adulto , Brasil/epidemiología , Femenino , Humanos , Masculino , Embarazo , Adulto Joven
3.
PLoS One ; 15(10): e0239951, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33002081

RESUMEN

Sri Lanka has a low-level HIV epidemic. This study aims to provide evidence on HIV, syphilis and hepatitis B (HBV) prevalence, sexual risk behaviours and utilisation of HIV prevention interventions among female sex workers (FSW) in the cities Colombo, Galle, and Kandy. Using respondent-driven sampling (RDS), we recruited a total of 458 FSW in Colombo, 360 in Galle and 362 in Kandy from November 2017 to March 2018. Participants provided biological specimens for testing for infections and completed a behavioural questionnaire. We found no HIV nor HBV infections in Galle and Kandy, and low HIV (0.4%) and HBV surface antigen (0.6%) prevalence in Colombo. FSW in Colombo had higher positivity on Treponema pallidum-particle agglutination test (8.4%) compared to Galle (2.0%) and Kandy (2.5%). About two thirds of FSW heard of HIV in each of the cities. Around 90% of FSW used condom at last sex with a client in both Colombo and Galle, but considerably less in Kandy (57.1%). However, lower proportion of FSW used condoms every time during sex with clients in the past 30 day: 22.9% of FSW in Colombo, 26.6% in Kandy and 68.4% in Galle. Across cities, 17.5%-39.5% of FSW reported being tested for HIV in the past 12 months or knowing HIV positive status. The commonest reasons for never testing for HIV was not knowing where to test (54.2% in Colombo, 41.8% in Galle, 48.1% in Kandy) followed by inconvenient testing location (23.7% in Colombo and 31.1% in Kandy). HIV has not yet been firmly established among FSW in three cities in Sri Lanka, but the vulnerability towards HIV and STIs is substantial. HIV interventions should be intensified by expanding community-based HIV testing approaches, increasingawareness of HIV risks and addressing socio-structural vulnerabilities of FSW to HIV.


Asunto(s)
Seropositividad para VIH/epidemiología , Hepatitis B/epidemiología , Trabajadores Sexuales/estadística & datos numéricos , Sífilis/epidemiología , Serodiagnóstico del SIDA/estadística & datos numéricos , Adolescente , Adulto , Femenino , Humanos , Persona de Mediana Edad , Sri Lanka , Serodiagnóstico de la Sífilis/estadística & datos numéricos , Sexo Inseguro/estadística & datos numéricos
4.
BMC Infect Dis ; 20(1): 692, 2020 Sep 21.
Artículo en Inglés | MEDLINE | ID: mdl-32957955

RESUMEN

BACKGROUND: Syphilis has seen an increased incidence in recent years and can have serious and irreversible consequences if left un-diagnosed and untreated. This case report describes a presentation of syphilis and acute kidney injury - a scenario sparsely described in existing literature. CASE PRESENTATION: This 43-year old Man who has Sex with Men (MSM) presented to the emergency department with a 3-week history of vomiting and headaches, progressing to include pyrexia. These symptoms started following his return from a 2-week cruise in Central America throughout which he had been well. He had a background of well-controlled human immunodeficiency virus (HIV). On admission he had an Acute Kidney Injury (AKI) stage 3, without hydronephrosis, presumed to be pre-renal. Leptospirosis, the main differential, was negative serologically. 'Pyrexia of unknown origin' testing was performed, and cefuroxime commenced. Later in the admission, syphilis testing indicated an acute infection and he completed a full treatment course of benzylpenicillin. This, alongside intravenous fluids, resulted in symptom and renal resolution in 9 days and restoration of renal function. CONCLUSIONS: Renal complications in syphilis are rare, furthermore the majority of those documented occur in latent syphilis and are irreversible. There are limited numbers of other documented cases of AKI in acute syphilis, which like the gentleman in this case were reversible and did not lead to permanent kidney damage. This case adds to the knowledge base of AKI in initial presentation of syphilis. It also demonstrates not only the importance of taking a sexual history in patients with new infective symptoms but that testing for syphilis in at-risk groups regardless of history should be performed given its rising incidence. These considerations by physicians can lead to prompt diagnosis and management of syphilis and improve patient care and long-term outcomes.


Asunto(s)
Lesión Renal Aguda/etiología , Sífilis/complicaciones , Lesión Renal Aguda/microbiología , Adulto , Antibacterianos/uso terapéutico , Fiebre/etiología , Infecciones por VIH/tratamiento farmacológico , Homosexualidad Masculina , Humanos , Masculino , Penicilina G/uso terapéutico , Conducta Sexual , Minorías Sexuales y de Género , Sífilis/diagnóstico , Sífilis/tratamiento farmacológico , Serodiagnóstico de la Sífilis , Viaje
5.
Sheng Wu Gong Cheng Xue Bao ; 36(8): 1672-1678, 2020 Aug 25.
Artículo en Chino | MEDLINE | ID: mdl-32924365

RESUMEN

To investigate the detection threshold of Treponema pallidum specific antibody method by chemiluminescent immunoassay (CLIA) in Siemens ADVIA Centaur XP for Syphilis serological test, and compare with the results derived from CMIA, TP-WB and TPPA method. The result can serve as reference for the application of CLIA. In total 30 887 samples screened by Treponema pallidum specific antibody method were collected by Abbott architect i2000 CMIA from July 2018 to July 2019 in Yanda Hospital of Hebei Province. We selected 153 patients with the ratio of sample absorbance to critical value (S/CO) of 1-9 by CMIA screening of Treponema pallidum specific antibody as the research objects. The reverse sequence of syphilis serological detection was adopted, and TP-WB and TPPA were used as the confirmation methods respectively. MedCalc software was used to analyze the results of ROC curve, and the cut-off value was obtained. Chi square test was used to test the difference significance of counting data. The detection results of Treponema pallidum specific antibody in the same batch of serum samples were unequal by different methods. There was no significant difference between CLIA method and TPPA method, but significant difference between CLIA method with TP-WB method and CMIA method was found. TPPA test results and TP-WB test results were taken as gold standards, ROC curve analysis showed that the best diagnostic cutoff value of CLIA method was 4.01 and 16.06, respectively, and the area under the curve was 0.961 and 0.838. The suggested cutoff value of CLIA method is quite different when using different syphilis serological test methods as the gold standard, Therefore, when the S/CO value determined by CLIA is between 1.00 to 16.06, TP-WB method should be recommended as the first choice in laboratory serological test for recheck and confirmation to avoid clinical misdiagnosis.


Asunto(s)
Anticuerpos Antibacterianos , Serodiagnóstico de la Sífilis , Treponema pallidum , Anticuerpos Antibacterianos/sangre , Humanos , Mediciones Luminiscentes , Serodiagnóstico de la Sífilis/métodos , Serodiagnóstico de la Sífilis/normas
7.
Klin Lab Diagn ; 65(8): 521-528, 2020 Jul 20.
Artículo en Ruso | MEDLINE | ID: mdl-32762197

RESUMEN

In medical organizations special forms (approved by orders of the Ministry of health and the Central statistical office) are used to present the results of laboratory examination of the population for syphilis. Currently, these forms include laboratory technologies that do not meet modern standards. In 2018-2019 the normative documents, regulating the use of accounting forms of medical documentation in the public health system, and the practice of using forms with the results of laboratory examination for syphilis were studied in 35 branches of 3 medical organizations of the Lyubertsy district of the Moscow region and in 18 branches of Moscow city was conducted. It is established that in the medical organizations of Moscow and the Moscow region are used the different forms of the registration forms approved by orders of the Ministry of health of the USSR which have now become invalid. The structure of the forms with the results of the population survey for syphilis does not correspond to the applied modern technologies and standards of the survey. On the basis of requirements of modern regulatory documents two new forms of registration forms for the presentation of results of laboratory researches have been developed, within 1,5 years tested and offered for application: one form at screening of the population for syphilis and another - for performing verification examinations for diagnostics and the subsequent clinical and serological supervision.


Asunto(s)
Sífilis , Humanos , Laboratorios , Tamizaje Masivo , Moscú , Encuestas y Cuestionarios , Sífilis/diagnóstico , Sífilis/epidemiología , Serodiagnóstico de la Sífilis
8.
PLoS One ; 15(8): e0237949, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32817658

RESUMEN

Syphilis, caused by Treponema pallidum ssp. pallidum (TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.


Asunto(s)
Reacción en Cadena de la Polimerasa , Serodiagnóstico de la Sífilis/métodos , Sífilis/diagnóstico , Treponema/aislamiento & purificación , Humanos , Estudios Retrospectivos , Sífilis/sangre , Treponema/genética , Treponema/inmunología , Treponema/fisiología
9.
BMC Infect Dis ; 20(1): 455, 2020 Jun 29.
Artículo en Inglés | MEDLINE | ID: mdl-32600260

RESUMEN

BACKGROUND: Men who have sex with men (MSM) bear a high burden of syphilis infection. Expanding syphilis testing to improve timely diagnosis and treatment is critical to improve syphilis control. However, syphilis testing rates remain low among MSM, particularly in low- and middle-income countries. We describe the protocol for a randomised controlled trial (RCT) to assess whether provision of syphilis self-testing services can increase the uptake of syphilis testing among MSM in China. METHODS: Four hundred forty-four high-risk MSM will be recruited online and randomized in a 1:1:1 ratio to (1) standard syphilis self-testing arm; (2) a self-testing arm program enhanced with crowdsourcing and a lottery-based incentive, and (3) a standard of care (control). Self-testing services include a free syphilis self-test kit through the mail at monthly intervals. Participants in the lottery incentive arm will additionally receive health promotion materials generated from an open crowdsourcing contest and be given a lottery draw with a 10% chance to win 100 RMB (approximately 15 US Dollars) upon confirmed completion of syphilis testing. Syphilis self-test kits have step-by-step instructions and an instructional video. This is a non-blinded, open-label, parallel RCT. Participants in each arm will be followed-up at three and 6 months through WeChat (a social media app like Facebook messenger). Confirmation of syphilis self-test use will be determined by requiring participants to submit a photo of the used test kit to study staff via secure data messaging. Both self-testing and facility-based testing will be ascertained by sending a secure photographic image of the completed kit through an existing digital platform. The primary outcome is the proportion of participants who tested for syphilis in the past 3 months. DISCUSSION: Findings from this study will provide much needed insight on the impact of syphilis self-testing on promoting routine syphilis screening among MSM. The findings will also contribute to our understanding of the safety, effectiveness and acceptability of syphilis self-testing. These findings will have important implications for self-testing policy, both in China and internationally. TRIAL REGISTRATION: ChiCTR1900022409 (10 April, 2019).


Asunto(s)
Pruebas Diagnósticas de Rutina/métodos , Promoción de la Salud/métodos , Homosexualidad Masculina , Inmunoensayo/métodos , Tamizaje Masivo/métodos , Minorías Sexuales y de Género , Serodiagnóstico de la Sífilis/métodos , Sífilis/diagnóstico , Treponema pallidum/inmunología , Serodiagnóstico del SIDA/métodos , China , Colaboración de las Masas/métodos , Pruebas Diagnósticas de Rutina/efectos adversos , Estudios de Seguimiento , Infecciones por VIH/diagnóstico , Infecciones por VIH/virología , VIH-1/inmunología , VIH-2/inmunología , Humanos , Masculino , Tamizaje Masivo/efectos adversos , Motivación , Medios de Comunicación Sociales , Sífilis/microbiología , Serodiagnóstico de la Sífilis/efectos adversos
10.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(3): 956-960, 2020 Jun.
Artículo en Chino | MEDLINE | ID: mdl-32552964

RESUMEN

OBJECTIVE: To Establish the shielding threshold value of TP antibody ELISA for unpaid blood donors, so as to shield true positive blood donors from returning to team management. METHODS: The real serological status of 517 samples with anti-TP ELISA reactivity was determined by confirmation test of Treponema pallidum particle agglutination (TPPA). The shielding threshold of TP antibody was preliminarily determined by using 99% specificity of ROC and 95% positive predictive value of percentile method, respectively. 283 TP antibody reactivity specimens routinely tested in our laboratory were selected to determine the applicability of the initial shielding values obtained by the two methods, and finally to determine the shielding threshold values of TP antibody donors. RESULTS: The specific S/CO values of reagent A 99% were 13.33-16.18, that of reagent B 99% was 6.34, that of reagent B 99% was 13.17-19.85, and that of 95% was 6.62. Empirical evidence: 99% specific threshold shielding true positive rates of reagents A and B were 100%, 95% positive expected value shielding true positive rates were 98.4%, 99%. Final determination of 99% specific shielding threshold as a low value of blood donors shielding threshold. The shielding limits of reagent A and B were 13.33 and 13.17. CONCLUSION: The shielding threshold of TP antibody ELISA for blood donors established in this study can help to reduce the number of blood donors returning to team management.


Asunto(s)
Donantes de Sangre , Ensayo de Inmunoadsorción Enzimática , Humanos , Sífilis , Serodiagnóstico de la Sífilis , Treponema pallidum
11.
Cerebrovasc Dis ; 49(3): 301-306, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32570248

RESUMEN

BACKGROUND AND AIMS: Syphilis and stroke are high prevalent diseases in south Brazil and estimates of concomitance and possible role of syphilis in acute stroke are lacking. Our aims are to estimate the prevalence of syphilis and neurosyphilis (NS) in a cohort of tertiary stroke center. METHODS: We reviewed all hospital records of stroke/transitory ischemic attack (TIA) using International Classification of Diseases, 10th revision, at discharge, frequency of syphilis screen, serology positivity, cerebrospinal fluid (CSF) analysis, and prevalence of NS in this stroke population applying CDC criteria. RESULTS: Between 2015 and 2016, there were 1,436 discharges for cerebrovascular events and in 78% (1,119) of these cases, some syphilis screening was performed. We have found a frequency of positive serology for syphilis of 13% (143/1,119), and higher stroke severity was the main determinant for non-screening. Applying standard NS criteria, 4.7% (53/1,119) cases with CSF analysis had NS diagnosis: 8 based on CSF-Venereal Disease Research Laboratory (VDRL) positive and 45 based on abnormal CSF white cells or protein, but CSF VDRL negative. NS VDRL positive cases were younger, had higher serum VDRL title, had more frequent HIV infection, and received NS treatment more often. Demographic and clinical characteristics were not different between NS VDRL negative and non-NS cases. CONCLUSION: Positive syphilis serology is frequent in patients with acute stroke/TIA in our region. Acute post-stroke CSF abnormalities make the diagnosis of NS difficult in the context of CSF VDRL negative.


Asunto(s)
Ataque Isquémico Transitorio/epidemiología , Tamizaje Masivo , Neurosífilis/epidemiología , Accidente Cerebrovascular/epidemiología , Anciano , Brasil/epidemiología , Femenino , Humanos , Ataque Isquémico Transitorio/líquido cefalorraquídeo , Ataque Isquémico Transitorio/diagnóstico , Masculino , Persona de Mediana Edad , Neurosífilis/líquido cefalorraquídeo , Neurosífilis/diagnóstico , Valor Predictivo de las Pruebas , Prevalencia , Factores de Riesgo , Accidente Cerebrovascular/líquido cefalorraquídeo , Accidente Cerebrovascular/diagnóstico , Serodiagnóstico de la Sífilis
13.
Int J STD AIDS ; 31(7): 613-618, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32366180

RESUMEN

The sexual health services in the Birmingham and Solihull area of the United Kingdom, called Umbrella, has been offering home-based testing for sexually transmitted infections to patients since August 2015. The aim of this service evaluation was to evaluate the uptake, return rate and new diagnosis rates of home-based testing in comparison with clinic-based testing for human immunodeficiency virus (HIV), syphilis (STS) and hepatitis B. Home-based testing, although popular, had low uptake amongst high-risk groups such as men who have sex with men (MSM), compared to the clinic-based group (1% versus 11%, p < 0.001). This resulted in low positivity rates for HIV (0.02%) and STS (0.17%) and no new cases of hepatitis B in the home-based group. Therefore, our results show that home-based testing is not a cost-effective method of testing for HIV and likely this is also the case for hepatitis B and STS. Our recommendation would be to encourage uptake of home-based testing in high-risk groups such as MSM and Black Africans to improve the diagnosis rates of HIV, STS and hepatitis B. Alternatively, the continuation of home-based blood testing in the Birmingham and Solihull area will need to be reviewed by Umbrella as a cost-saving strategy for the service in the future.


Asunto(s)
Instituciones de Atención Ambulatoria/estadística & datos numéricos , Prestación de Atención de Salud/estadística & datos numéricos , Infecciones por VIH/diagnóstico , Infecciones por VIH/prevención & control , Hepatitis B/diagnóstico , Hepatitis B/prevención & control , Tamizaje Masivo/métodos , Autocuidado/estadística & datos numéricos , Enfermedades de Transmisión Sexual/diagnóstico , Enfermedades de Transmisión Sexual/prevención & control , Sífilis/diagnóstico , Sífilis/prevención & control , Adolescente , Adulto , Autoevaluación Diagnóstica , Femenino , Infecciones por VIH/psicología , Hepatitis B/psicología , Virus de la Hepatitis B , Humanos , Masculino , Estudios Retrospectivos , Enfermedades de Transmisión Sexual/psicología , Sífilis/psicología , Serodiagnóstico de la Sífilis , Treponema pallidum , Reino Unido , Adulto Joven
14.
Int J STD AIDS ; 31(7): 699-701, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32362198

RESUMEN

Annular syphilis may range from mildly raised lesions with scaly borders to verrucous plaques. Localized annular syphilis on the genitalia has been rarely reported in HIV-negative cases. This paper reports a case of annular secondary syphilis on the penis. Dermoscopy showed peripheral dotted and short linear vessels and white scaling with a relatively clear central area in an erythematous annular plaque. Histopathology revealed mild hyperkeratosis, parakeratosis, psoriasiform acanthosis, and focal basal vacuolar degeneration with lichenoid, perivascular, and periadnexal infiltrate of lymphohistiocytes and plasma cells in the superficial dermis. Silver stain showed several spirochetes in the lower epidermis and superficial dermis. Electron microscopy revealed a few intercellular and intracytoplasmic spirochetes in the basal epidermis and free spirochetes in the papillary dermis. Rapid plasma reagin and Treponema pallidum particle agglutination assays were positive. The lesions disappeared after intramuscular benzathine penicillin, with no relapse at six-month follow-up.


Asunto(s)
Pene/patología , Sífilis Cutánea/patología , Sífilis/diagnóstico , Treponema pallidum/aislamiento & purificación , Adulto , Humanos , Masculino , Microscopía Electrónica , Penicilina G Benzatina/uso terapéutico , Sífilis/tratamiento farmacológico , Serodiagnóstico de la Sífilis , Resultado del Tratamiento
15.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(2): 641-645, 2020 Apr.
Artículo en Chino | MEDLINE | ID: mdl-32319409

RESUMEN

OBJECTIVE: To investigate the prevalence of syphilis infection in voluntary blood donors in Foshan City, so as to provide reference for improving the infusion blood safety strategy of city. METHODS: A total of 657 382 voluntary blood donors in Foshan City from January 2008 to December 2017 were selected. The blood samples were tested for treponema pallidum. The prevalence trends, the detection rate of treponema pallidum infection and epidemiological data were statistically analyzed. Enzyme-linked immunosorbent assay (ELISA) was performed for antibody screening in blood samples. The two-hole retest was then performed for the samples which were positive in initial test, meanwhile, they were also confirmed by the treponema hemagglutination blood coagulation test (TPHA). RESULTS: First, the annual infection rate of treponema pallidum in Foshan City from January 2008 to December 2017 was 0.43%, 0.40%, 0.41%, 0.43%, 0.50%, 0.32%, 0.36%, 0.32%, 0.37% and 0.32%, respectively. A total of 2502 cases of treponema pallidum infection were detected, the overall infection rate was 0.38%, and the infection rate decreased year by year (x2=23.826, P<0.05). Second, among the 657 382 samples of voluntary blood donors, 2 502 were showed the positive treponema pallidum antibody (0.38%). In 2015-2017, 791 donors were showed positive treponema pallidum antibody, 676 case out of which were confirmed positive, 0.34% with the positive coincidence rate of 85.46%. Third, this group of 676 syphilis infection cases were male-dominated (0.35%, 577/164 484). The age distribution was 18-53 years old, and the average age was 32 years, among which 35-44 years old accounted for 0.33% (138/41 858). The education level of in donors with treponema pallidom positive mainly ranked at low or lower levels under janior college (0.32%, 571/180 917). The occupation is mainly outside work and company staff (0.39%, 452/115 101). The marital status is mainly unmarried (0.29%, 377/130 905). The place of origin is mainly non-local (0.29%,570/198 063). CONCLUSION: The positive rate of treponema pallidum shows a downward trend in 2008-2017. Among the individuals with comfirmed TP antibody positive in 2015-2017, the non-local, unmarried and low educated males are main population, moreover more than half of them are migrant worker or company employeers.


Asunto(s)
Donantes de Sangre , Sífilis , Adolescente , Adulto , Anticuerpos Antibacterianos , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Persona de Mediana Edad , Serodiagnóstico de la Sífilis , Treponema pallidum , Adulto Joven
16.
Int J Gynaecol Obstet ; 150(1): 103-107, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32246772

RESUMEN

OBJECTIVE: To evaluate a treponemal enzyme-linked immunosorbent assay (ELISA) as an alternative screening test for syphilis in pregnant women. METHODS: A cross-sectional study of diagnostic test accuracy was carried out in a large volume laboratory from a tertiary care center. A total of 416 serum samples, including 102 archived syphilis Treponema pallidum hemagglutination (TPHA)-positive samples and 314 samples from pregnant women, were used to determine the sensitivity and specificity of ELISA. All the samples were subjected to Venereal Disease Research Laboratory (VDRL), rapid plasma reagin (RPR), ELISA, and TPHA tests. Performance characteristics of VDRL, RPR, and ELISA were calculated with TPHA as a reference standard test. RESULTS: VDRL and RPR exhibited higher false positivity of 10.5% and 9.6%, respectively, compared to 2.5% by ELISA. The sensitivity and specificity of ELISA were 98% and 97.5%, of VDRL were 71.6% and 89.5%, and of RPR were 73.5% and 90.5%, respectively. Moreover, ELISA had an excellent agreement (kappa=0.9) with TPHA compared to VDRL/RPR which had a moderate agreement (kappa=0.6) only. CONCLUSION: ELISA has the potential to replace VDRL/RPR as a screening test for syphilis in centers that can perform ELISA, especially for antenatal screening.


Asunto(s)
Ensayo de Inmunoadsorción Enzimática/normas , Complicaciones Infecciosas del Embarazo/diagnóstico , Diagnóstico Prenatal/métodos , Serodiagnóstico de la Sífilis/métodos , Sífilis/diagnóstico , Adulto , Estudios Transversales , Femenino , Humanos , Embarazo , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Treponema pallidum/aislamiento & purificación
19.
Klin Lab Diagn ; 65(1): 16-23, 2020.
Artículo en Ruso | MEDLINE | ID: mdl-32155002

RESUMEN

The aim of the study was to investigate the characteristics of immunoarrays (microarrays) produced by co-polymerization immobilization and non-contact printing techniques for enhancing the capacities of syphilis diagnostics. In diagnostic context immunoarrays of both protein immobilization techniques have shown high sensitivity and specificity together with potency to differentiate syphilis stages in serologic assays. The article discloses the advantages and limitations of non-contact printing techniques as well as the results and problems revealed in the study. Solution of these problems in future may provide the development of new serodiagnostic tools with higher accuracy of the results.


Asunto(s)
Inmunoensayo/métodos , Serodiagnóstico de la Sífilis/métodos , Sífilis/diagnóstico , Humanos , Polimerizacion , Impresión Tridimensional , Sensibilidad y Especificidad , Treponema pallidum
20.
Infect Dis Poverty ; 9(1): 26, 2020 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-32160927

RESUMEN

BACKGROUND: Yaws is a chronic relapsing disease caused by Treponema pallidum subspecies pertunue, which can result in severe disability and deformities. Children below the age of 15 years in resource-poor communities are the most affected. Several non-specific factors facilitate the continuous transmission and resurgence of the disease. Endemic communities in rural Ghana continue to report cases despite the roll out of several intervention strategies in the past years. The objective of this study was to determine the factors associated with cutaneous ulcers among children in two yaws-endemic districts in Ghana. METHODS: A community-based unmatched 1:2 case-control study was conducted among children between 1 and 15 years. Data on socio-demographic, environmental and behavioral factors were collected using a structured questionnaire. Active case search and confirmation was done using the Dual Path Platform (DPP) Syphilis Screen and Confirm test kit. Data were analyzed using STATA 15. Logistic regression was done to determine the exposures that were associated with yaws infection at 0.05 significant level. RESULTS: Sixty-two cases and 124 controls were recruited for the study. The adjusted multivariable logistic regression model showed that yaws infection was more likely among individuals who reside in overcrowded compound houses (aOR = 25.42, 95% CI: 6.15-105.09) and with poor handwashing habits (aOR = 6.46, 95% CI: 1.89-22.04). Male (aOR = 4.15, 95% CI: 1.29-13.36) and increasing age (aOR = 5.90, 95% CI: 1.97-17.67) were also associated with yaws infection. CONCLUSIONS: Poor personal hygiene, overcrowding and lack of access to improved sanitary facilities are the factors that facilitate the transmission of yaws in the Awutu Senya West and Upper West Akyem districts. Yaws was also more common among males and school-aged children. Improving living conditions, access to good sanitary facilities and encouraging good personal hygiene practices should be core features of eradication programs in endemic communities.


Asunto(s)
Población Rural , Úlcera Cutánea/microbiología , Treponema pallidum/aislamiento & purificación , Buba , Adolescente , Estudios de Casos y Controles , Niño , Preescolar , Aglomeración , Enfermedades Endémicas , Femenino , Ghana/epidemiología , Humanos , Higiene , Lactante , Masculino , Vigilancia de la Población , Úlcera Cutánea/diagnóstico , Úlcera Cutánea/epidemiología , Encuestas y Cuestionarios , Serodiagnóstico de la Sífilis , Buba/diagnóstico , Buba/epidemiología , Buba/prevención & control , Buba/transmisión
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