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1.
Wiad Lek ; 74(3 cz 1): 535-538, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33813464

RESUMEN

OBJECTIVE: The aim: To increase the effectiveness of treatment of sinusitis polyposa patients, develop and introduce a new non-traditional complex method of influencing several links of the pathogenesis of this disease, including laser therapy and laser puncture, used in the postoperative period. PATIENTS AND METHODS: Materials and methods: In 60 rhinosinusitis polyposa patients the indicators of lipid peroxidation and the state of antioxidant protection were studied. Depending on the type of treatment, the patients were divided into two groups: the first included 30 people who underwent traditional surgical intervention, and the second - 30 people who used complex therapy, including in the postoperative period endonasal laser exposure and laser puncture. Methods: clinical data, the functional state of the nasal mucosa, indicators of lipid peroxidation (hydroperoxide, malondialdehyde) and antioxidant activity (glutathione peroxidase, glutathione reductase, reduced glutathione, superoxide cismutase, catalase) of erythrocyte membranes and blood serum were studied. RESULTS: Results: The proposed complex method for treating lipids of erythrocyte membranes and serum, which includes endonasal surgical intervention, endonasal laser therapy and laser puncture leads to the normalization of physiological functions of the nose, activates antioxidant protection and reduces the peroxide activity of lipids in the membranes of erythrocytes and blood serum. CONCLUSION: Conclusions: When examining patients in the long-term (after 1 year) period, a significant improvement in 85.7% of cases, an improvement in 10.7%, and absence of effect in 3.6% was achieved. Thus, the proposed method of therapy can be recommended for widespread use in medical institutions.


Asunto(s)
Sinusitis , Superóxido Dismutasa , Antioxidantes , Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Peroxidación de Lípido , Sinusitis/terapia , Superóxido Dismutasa/metabolismo
2.
Pestic Biochem Physiol ; 174: 104825, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33838718

RESUMEN

Insecticide exposure typically leads to abnormally high levels of reactive oxygen species (ROS) and oxidative damage in insects. Superoxide dismutases (SODs) are potent antioxidant enzymes for ROS scavenging that are essential to protect insects against insecticide-induced oxidative injury. The small white butterfly, Pieris rapae, is an economically important lepidopteran pest of cruciferous crops, and the anthranilic diamide insecticide chlorantraniliprole is widely used to control this organism. However, whether chlorantraniliprole causes oxidative stress, and whether SODs are involved in ROS scavenging, remains unclear in P. rapae. In this study, an intracellular copper/zinc SOD (designated PrSOD1) gene was identified and characterised in P. rapae. The gene consists of four exons and three introns, and the PrSOD1 protein encoded by the gene has typical highly conserved features of CuZnSODs, including two signature motifs and seven Cu/Zn-interacting residues. Transcription of PrSOD1 was highest in the larval fat body and at the fifth-instar larval stage. Recombinant PrSOD1 protein expressed in Escherichia coli displayed antioxidant activity and high thermal and pH stability, confirming that PrSOD1 encodes a functional enzyme. Exposure to three sublethal doses of chlorantraniliprole for 6, 12 or 24 h resulted in significantly increased malondialdehyde concentration in P. rapae larvae, indicating insecticide-induced oxidative stress. Furthermore, both PrSOD1 transcription levels and CuZnSOD activity were quickly (6 and 12 h, respectively) upregulated in larvae subjected to chlorantraniliprole, strongly suggesting that PrSOD1 plays an important role in protecting against oxidative damage and possibly chlorantraniliprole tolerance in P. rapae.


Asunto(s)
Mariposas Diurnas , Animales , Cobre/toxicidad , Estrés Oxidativo , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Zinc , ortoaminobenzoatos
3.
Ecotoxicol Environ Saf ; 214: 112049, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33647852

RESUMEN

The disposal of untreated sanitary sewage in the soil has several consequences for human health and leads to environmental risks; thus, it is necessary investigating, monitoring and remediating the affected sites. The aims of the current study are to evaluate ecotoxicological effects on Eisenia andrei earthworms exposed to soil subjected to sources of sanitary sewage discharge and to investigate whether prevention values established by the Brazilian legislation for soil quality, associated with the incidence of chemical substances in it, are satisfactory enough to assure the necessary quality for different organisms. Earthworms' behavior, reproduction, acetylcholinesterase activity, catalase, superoxide dismutase and malondialdehyde levels were evaluated. The reproduction and behavior of earthworms exposed to sanitary sewage were adversely affected. Increased superoxide dismutase and catalase activity acted as antioxidant defense mechanism. Significantly increased lipid peroxidation levels and acetylcholinesterase activity inhibition have indicated lipid peroxidation in cell membrane and neurotransmission changes, respectively. Results have confirmed that sanitary sewage induced oxidative stress in earthworms. In addition, based on biochemical data analysis, the integrated biomarker response (IBR) has evidenced different toxicity levels in earthworms between the investigated points. Finally, results have indicated that effluents released into the soil, without proper treatment, lead to contaminant accumulation due to soil saturation and it can hinder different processes and biological development taking place in the soil. In addition, the current study has shown that physical-chemical analyses alone are not enough to assess soil quality, since it is also requires adopting an ecotoxicological approach. Brazilian legislation focused on soil quality must be revised and new guiding values must be proposed.


Asunto(s)
Oligoquetos/fisiología , Contaminantes del Suelo/análisis , Animales , Antioxidantes/metabolismo , Brasil , Catalasa/metabolismo , Ecotoxicología , Contaminación Ambiental/análisis , Humanos , Malondialdehído/metabolismo , Oligoquetos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Aguas del Alcantarillado , Suelo/química , Superóxido Dismutasa/metabolismo
4.
J Med Life ; 14(1): 32-36, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33767782

RESUMEN

Acute small bowel obstruction remains one of the most challenging nosologies in emergency surgery, leading to a pronounced imbalance between lipid peroxidation and the antioxidant defense system. We aimed to study changes in the anti- and prooxidant status of serum and small intestine wall in an experiment modeling acute small bowel obstruction. The control group included 11 rats, and the main group included 42 rats (simulation of mechanical bowel obstruction on day 1 was conducted in 14 rats, on day 2 - in 12 rats, on day 3 - in 16 rats). Acute small bowel obstruction was modeled by ligation. Serum analysis and removal of the small intestinal wall were performed on days 1, 2, and 3. Indicators of lipid peroxidation and antioxidant protection were determined by the spectrophotometric method, and the imbalance between lipid peroxidation and antioxidant protection gradually increased from 1 to 3 days of observation. On day 3, the low level of aldehyde increased 1.3 times, and the level of superoxide dismutase decreased 1.2 times compared to the control group. Pathophysiological changes in the wall of the small intestine are caused by the activation of lipid peroxidation and the exhaustion antioxidant protection, whereby the degree of their severity increases depending on the increase in time from the moment of modeling of acute obstruction of the small intestine.


Asunto(s)
Antioxidantes/metabolismo , Obstrucción Intestinal/metabolismo , Intestino Delgado/patología , Enfermedad Aguda , Animales , Intestino Delgado/cirugía , Peroxidación de Lípido , Masculino , Ratas , Superóxido Dismutasa/metabolismo
5.
Int J Mol Sci ; 22(4)2021 Feb 17.
Artículo en Inglés | MEDLINE | ID: mdl-33671292

RESUMEN

Anticancer drugs, such as fluorouracil (5-FU), oxaliplatin, and doxorubicin (Dox) are commonly used to treat colorectal cancer (CRC); however, owing to their low response rate and adverse effects, the development of efficient drug delivery systems (DDSs) is required. The cellular prion protein PrPC, which is a cell surface glycoprotein, has been demonstrated to be overexpressed in CRC, however, there has been no research on the development of PrPC-targeting DDSs for targeted drug delivery to CRC. In this study, PrPC aptamer (Apt)-conjugated gold nanoparticles (AuNPs) were synthesized for targeted delivery of Dox to CRC. Thiol-terminated PrPC-Apt was conjugated to AuNPs, followed by hybridization of its complementary DNA for drug loading. Finally, Dox was loaded onto the AuNPs to synthesize PrPC-Apt-functionalized doxorubicin-oligomer-AuNPs (PrPC-Apt DOA). The PrPC-Apt DOA were spherical nanoparticles with an average diameter of 20 nm. Treatment of CRC cells with PrPC-Apt DOA induced reactive oxygen species generation by decreasing catalase and superoxide dismutase activities. In addition, treatment with PrPC-Apt DOA inhibited mitochondrial functions by decreasing the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, complex 4 activity, and oxygen consumption rates. Compared to free Dox, PrPC-Apt DOA decreased proliferation and increased apoptosis of CRC cells to a greater degree. In this study, we demonstrated that PrPC-Apt DOA targeting could effectively deliver Dox to CRC cells. PrPC-Apt DOA can be used as a treatment for CRC, and have the potential to replace existing anticancer drugs, such as 5-FU, oxaliplatin, and Dox.


Asunto(s)
Aptámeros de Nucleótidos/química , Neoplasias Colorrectales/tratamiento farmacológico , Doxorrubicina/administración & dosificación , Doxorrubicina/uso terapéutico , Sistemas de Liberación de Medicamentos , Oro/química , Nanopartículas del Metal/química , Proteínas Priónicas/química , Apoptosis/efectos de los fármacos , Catalasa/metabolismo , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Doxorrubicina/farmacología , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Mitocondrias/metabolismo , Coactivador 1-alfa del Receptor Activado por Proliferadores de Peroxisomas gamma/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Esferoides Celulares/efectos de los fármacos , Esferoides Celulares/metabolismo , Superóxido Dismutasa/metabolismo
6.
Medicine (Baltimore) ; 100(11): e23666, 2021 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-33725925

RESUMEN

ABSTRACT: We aimed to investigate the effect of Kelch-like ECH-associated protein 1/NF-E2 p45-related factor 2 (Keap1/Nrf2) pathway on the biological function of trophoblast cells in oxidative stress model at the cellular level, and analyzed the expression level and clinical significance of Keap1/Nrf2 pathway and related antioxidant factors in placental tissues of Preeclampsia (PE) patients at clinical level. In present study, we found that under hypoxia/reoxygenation conditions, the activity of oxidative stress-related enzymes (CAT, GSH-Px, SOD) in HTR8/SVneo cells was significantly lower than that before treatment (P < .01). The activities of CAT, GSH-Px and SOD in HTR8/SVneo cells in SiRNA+H/R group decreased significantly (P < .01), indicating the important defense effect of Keap1/Nrf2 signaling pathway in oxidative stress. As a control group of Nrf2 SiRNA+H/R group, Si-NC+H/R group had CAT, GSH-Px and SOD activities decreasing, which was similar to that in H/R group. Moreover, the activities of oxidative stress-related active enzymes in patients with PE were further confirmed by detecting and comparing the activities of CAT, GSH-Px and SOD in placental tissues. The results showed that the activity of SOD (P < .001), GSH-Px (P < .01) and CAT (P < .01) in placental tissues of patients with PE were significant different from those of normal placental tissues. The expression level of Keap1 in placenta of patients with PE was slightly lower than that of normal placenta. While the expression of Nrf2 in placenta of patients with PE was significantly higher than that of normal placenta. HO-1 expression in placenta of patients with PE was significantly higher than that of normal placenta. These results implicate the importance of Keap-1/Nrf2 pathway in PE.


Asunto(s)
Hipertensión Inducida en el Embarazo/enzimología , Proteína 1 Asociada A ECH Tipo Kelch/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Estrés Oxidativo/fisiología , Preeclampsia/enzimología , Catalasa/metabolismo , China , Femenino , Glutatión Peroxidasa/metabolismo , Humanos , Placenta/citología , Embarazo , Superóxido Dismutasa/metabolismo , Trofoblastos/enzimología
7.
Ecotoxicol Environ Saf ; 214: 112067, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33640724

RESUMEN

Deltamethrin (DM) is a synthetic pyrethroid used for agricultural purposes to control insects. However, its extensive use contaminates the aquatic environment and results in serious health problems in aquatic organisms. Knowledge about the toxic effect of DM in freshwater prawns is limited; therefore, this study aims to assess the toxicity of DM in Macrobrachium rosenbergii based on multiple biomarkers. Four-day acute toxicity tests showed that DM was highly toxic to M. rosenbergii with the 24 h, 48 h, 72 h and 96 h LC50 values to be 1.919, 0.603, 0.539, and 0.449 µg/L, respectively. According to 96 h LC50, prawns were exposed to DM at three concentrations (0.02, 0.08, and 0.32 µg/L) for 4 days, and then moved into fresh water for decontamination to investigate the toxic effect of DM in M. rosenbergii. At low concentration (0.02 µg/L and 0.08 µg/L), DM did not cause obvious histopathological damage to hepatopancreas and gill tissue, while at high concentration (0.32 µg/L), the histopathological harm was serious and the damage did not recover to the initial level after 7-day decontamination. 0.02 µg/L DM exposure did not induce significant changes in most of the biomarkers except the increased lactate dehydrogenase (LDH) activity, lactic acid (LD) level, and the first increased then decreased mRNA expression of immune-related genes, indicating the stimulation of DM on energy production and immunity. 0.08 µg/L and 0.32 µg/L DM exposure resulted in varying degrees of damage on prawns, but overall, their toxic effects showed similar trends based on the biomarkers. Increase in malonaldehyde (MDA) and hydrogen peroxide (H2O2) content and decrease in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity after DM exposure demonstrated the oxidative stress caused by DM. The significantly increased acid phosphatase (ACP), alkaline phosphatase (AKP), LDH activity and LD level indicated hepatopancreatic dysfunction and respiration disruption. The first increased and then decreased expression pattern of immune-related genes indicated the immunosuppression caused by DM. After 7-day decontamination in freshwater, the activity/level of the biomarkers partly recovered. This study revealed the severe toxic effect of DM on Macrobrachium rosenbergii based on multiple biomarkers, providing fundamental knowledge for the establishment of DM toxicity assessment system with proper parameters in freshwater crustaceans.


Asunto(s)
Nitrilos/toxicidad , Palaemonidae/fisiología , Piretrinas/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Organismos Acuáticos/metabolismo , Biomarcadores/metabolismo , Agua Dulce , Branquias/metabolismo , Hepatopáncreas/efectos de los fármacos , Peróxido de Hidrógeno/metabolismo , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Palaemonidae/efectos de los fármacos , Piretrinas/farmacología , Superóxido Dismutasa/metabolismo
8.
Sci Total Environ ; 772: 145034, 2021 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-33571776

RESUMEN

Fish can be highly vulnerable to environmental pressures because they are exposed to oxidative stressors in the aquatic environment. Such stressors can affect the levels of antioxidant biomarkers against reactive oxygen species (ROS). With this study we investigated the oxidative stress ecology in Danube barbel (Barbus balcanicus) from the Barbucina creek (northeast Italy), a watercourse in the Collio winegrowing district. To do this, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activity was measured in gills, liver, and muscle, while metallothioneins (MT) and trace and rare earth elements (REEs) levels were determined in muscle. The effect of environmental factors (physicochemical parameters of water, trace elements and REEs) on oxidative stress biomarkers was thus assessed. High concentrations were determined for cerium (Ce), scandium (Sc), neodymium (Nd), lanthanum (La), yttrium (Y), and praseodymium (Pr) among the REEs. Among the trace elements, arsenic (As), copper (Cu), and mercury (Hg) levels were higher compared to published data, suggesting their role as stressors. The multiple linear regression (MLR) model showed a statistically significant association (R2 = 0.858; F = 10.07; p = 0.015) between As, Cu, Hg, and Pr and SOD activity in the gills, indicating a functional relationship between them. Differently, CAT activity was significantly higher in the liver, probably in response to long-term Cu contamination of the watercourse. This was confirmed by the MLR model that showed a significant association (R2 = 0.638; F = 8.152; p = 0.02) between the concentration of MT and of Cu. Our data show a biochemical defensive response by Danube barbel to the disturbances in the aquatic ecosystem of the Barbucina creek. These insights advance our understanding of the role and the effects of environmental factors as trace elements and REEs on oxidative stress in fish.


Asunto(s)
Cyprinidae , Contaminantes Químicos del Agua , Animales , Biomarcadores/metabolismo , Catalasa/metabolismo , Cyprinidae/metabolismo , Ecosistema , Branquias/metabolismo , Glutatión Transferasa/metabolismo , Italia , Estrés Oxidativo , Superóxido Dismutasa/metabolismo , Agua , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
9.
Int J Nanomedicine ; 16: 1231-1244, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33633448

RESUMEN

Background and Aim: Acute myeloid leukemia (AML), initiated and maintained by leukemia stem cells (LSCs), is often relapsed or refractory to therapy. The present study aimed at assessing the effects of nanozyme-like Fe3O4 nanoparticles (IONPs) combined with cytosine arabinoside (Ara-C) on LSCs in vitro and in vivo. Methods: The CD34+CD38-LSCs, isolated from human AML cell line KG1a by a magnetic activated cell sorting method, were treated with Ara-C, IONPs, and Ara-C+ IONPs respectively in vitro. The cellular proliferation, apoptosis, reactive oxygen species (ROS), and the related molecular expression levels in LSCs were analyzed using flow cytometry, RT-qPCR, and Western blot. The nonobese diabetic/severe combined immune deficiency mice were transplanted with LSCs or non-LSCs via tail vein, and then the mice were treated with Ara-C, IONPs and IONPs plus Ara-C, respectively. The therapeutic effects on the AML bearing mice were further evaluated. Results: LSCs indicated stronger cellular proliferation, more clone formation, and more robust resistance to Ara-C than non-LSCs. Compared with LSCs treated with Ara-C alone, LSCs treated with IONPs plus Ara-C showed a significant increase in apoptosis and ROS levels that might be regulated by nanozyme-like IONPs via improving the expression of pro-oxidation molecule gp91-phox but decreasing the expression of antioxidation molecule superoxide dismutase 1. The in vivo results suggested that, compared with the AML bearing mice treated with Ara-C alone, the mice treated with IONPs plus Ara-C markedly reduced the abnormal leukocyte numbers in peripheral blood and bone marrow and significantly extended the survival of AML bearing mice. Conclusion: IONPs combined with Ara-C showed the effectiveness on reducing AML burden in the mice engrafted with LSCs and extending mouse survival by increasing LSC's ROS level to induce LSC apoptosis. Our findings suggest that targeting LSCs could control the AML relapse by using IONPs plus Ara-C.


Asunto(s)
Citarabina/uso terapéutico , Leucemia Mieloide Aguda/tratamiento farmacológico , Leucemia Mieloide Aguda/patología , Células Madre Neoplásicas/patología , Especies Reactivas de Oxígeno/metabolismo , Animales , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Separación Celular , Citarabina/farmacología , Hemoglobinas/metabolismo , Humanos , Leucemia Mieloide Aguda/sangre , Recuento de Leucocitos , Ratones Endogámicos NOD , Ratones SCID , NADPH Oxidasa 2/metabolismo , Células Madre Neoplásicas/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Ensayos Antitumor por Modelo de Xenoinjerto
10.
Ecotoxicol Environ Saf ; 212: 111980, 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33545408

RESUMEN

This paper investigated the toxic effect and mechanism of ultrafine carbon black (UFCB) on splenocytes and enzymes in the digestive system. It was found that the toxicity of UFCB to splenocytes was dose-dependent. UFCB with a low concentration (<15 µg/mL) had no significant effect on splenocytes while UFCB with high concentration (>15 µg/mL) induced significant oxidative damage with increased content of reactive oxygen species (ROS) (134%) and malonaldehyde (MDA) (222.3%) along with the decreased activity of superoxide dismutase (SOD) (55.63%) and catalase (CAT) (87.73%). Analysis combined cellular and molecular levels indicated that UFCB induced splenocyte toxicity through oxidative stress. The interactions of UFCB with two important digestive enzymes, α-amylase and lipase, were also studied respectively. Results showed that the interaction of UFCB and the two enzymes altered the particle size and fluorescence intensity in both experimental systems. The formation of protein corona also resulted in the contraction of the polypeptide skeleton in both enzymes, which further inhibited their activity. Our work provided basic data on the toxicity of UFCB in the spleen and digestive system and fills the gap in the study of UFPs toxicity. CAPSULE: UFCB induced splenocyte toxicity and enzyme dysfunction through oxidative stress and protein corona formation respectively.


Asunto(s)
Hollín/toxicidad , Bazo/fisiología , Animales , Ratones , Estrés Oxidativo/efectos de los fármacos , Tamaño de la Partícula , Proteínas/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Bazo/efectos de los fármacos , Bazo/metabolismo , Superóxido Dismutasa/metabolismo
11.
Life Sci ; 272: 119243, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33607157

RESUMEN

High fat consumption leads to reactive oxygen species (ROS) which is associated with age-progressive neurological disorders. Cu/Zn superoxide dismutase (SOD1) is a critical enzyme against ROS. However, the relationship between SOD1 and the high-fat-induced ROS and neurodegeneration is poorly known. Here we showed that, upon treatment with a saturated fatty acid palmitic acid (PA), the SOD1 activity was decreased in mouse neuronal HT-22 cell line accompanied by elevation of ROS, but not in mouse microglial BV-2 cell line. We further showed that PA decreased the levels of copper chaperone for SOD1 (CCS) in HT-22 cells, which promoted the nuclear import of SOD1 and decreased its activity. We demonstrated that the reduction of CCS is involved in the PA-induced decrease of SOD1 activity and elevation of ROS. In addition, compared with the adult mice fed with a standard diet, the high-fat-diet adult mice presented an increase of plasma free fatty acids, reduction of hippocampal SOD1 activity and CCS, mitochondrial degeneration and long-term memory decline. Taken together, our findings suggest that the high-fat-induced lower CCS level is essential for SOD1 suppression which may be associated with neurodegeneration and cognitive decline.


Asunto(s)
Dieta Alta en Grasa/efectos adversos , Chaperonas Moleculares/metabolismo , Superóxido Dismutasa-1/metabolismo , Animales , Línea Celular , China , Cobre/metabolismo , Masculino , Memoria , Trastornos de la Memoria , Ratones , Ratones Endogámicos C57BL , Mitocondrias/metabolismo , Enfermedades Neurodegenerativas/fisiopatología , Ácido Palmítico/metabolismo , Ácido Palmítico/farmacología , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismo , Superóxido Dismutasa-1/fisiología
12.
Chem Biol Interact ; 338: 109402, 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33587916

RESUMEN

Cisplatin is an important antineoplastic drug used in multiple chemotherapeutic regimens but unfortunately causes serious toxic effects as ovarian and uterine toxicity. This study aimed to investigate the potential protective effect of resveratrol (RSV) against cisplatin-induced ovarian and uterine toxicity in female rats. Thirty-two female Wistar rats were divided randomly into four groups (n = 8 in each). Control group received oral normal saline for 28 days; RSV group received RSV (10 mg/kg; daily) via oral gavage; CIS group received a single dose of CIS (7 mg/kg; i.p.) on the 21st day; (CIS + RSV) group received both RSV and CIS by the same schedules and doses of RSV and CIS groups, respectively. Results demonstrated a significant decrease in MDA level and a significant increase in both glutathione content and activity of the antioxidant enzymes GPx, SOD, and CAT in the tissues of the ovary and uterus of CIS + RSV group in comparison to that of CIS group (P<0.05), also there are significantly decreased tissue levels of the proinflammatory cytokines and enzymes (NF-κB, IL-1ß, IL-6, TNF-α, COX-2, and iNOS), increased estradiol, progesterone, prolactin and decreased FSH serum levels in CIS + RSV group compared to CIS group (P < 0.05). Moreover, there is downregulation of tissues Cleaved Caspase-3, NF-κB and Cox-2 proteins as shown in Western blot analysis, also apoptosis was significantly inhibited, evidenced by downregulation of Bax and upregulation of Bcl-2 proteins, and the ovarian and uterine histological architecture and integrity were maintained in CIS + RSV group compared to CIS group. In conclusion, these findings indicate that RSV has beneficial effects in ameliorating cisplatin-induced oxidative stress, inflammation, and apoptosis in the ovarian and uterine tissues of female rats.


Asunto(s)
Apoptosis/efectos de los fármacos , Cisplatino/efectos adversos , Inflamación/patología , Ovario/patología , Estrés Oxidativo/efectos de los fármacos , Sustancias Protectoras/farmacología , Resveratrol/farmacología , Útero/patología , Animales , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Catalasa/metabolismo , Ciclooxigenasa 2/metabolismo , Citocinas/metabolismo , Estradiol/sangre , Femenino , Hormona Folículo Estimulante/sangre , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Malondialdehído/metabolismo , Modelos Biológicos , FN-kappa B/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Ovario/efectos de los fármacos , Progesterona/sangre , Prolactina/sangre , Carbonilación Proteica/efectos de los fármacos , Ratas Wistar , Superóxido Dismutasa/metabolismo , Proteína X Asociada a bcl-2/metabolismo
13.
Artículo en Chino | MEDLINE | ID: mdl-33535330

RESUMEN

Objective: To investigate the neurotoxicity of crotonaldehyde exposure in male rats and its possible mechanism of action. Methods: From July to October 2019, 24 specific pathogen-free male Wistar rats were randomly divided into control group and 2.5, 4.5, and 8.5 mg/kg exposure groups, with 6 rats in each group, and the rats in these groups were given oral administration of crotonaldehyde solution at doses of 0.0, 2.5, 4.5, and 8.5 mg/kg, respectively, 5 times a week for 90 consecutive days. Body weight was measured after exposure, and brain tissue and liver tissue were collected. The activity of acetylcholinesterase (AChE) in brain tissue and the level of acetylcholine (ACh) in liver tissue were measured; The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the levels of malondialdehyde (MDA) and reduced glutathione (GSH) in brain tissue were measured; ELISA was used to measure the levels of interleukin-6 (IL-6) , interleukin-1ß (IL-1ß) , and tumor necrosis factor-α (TNF-α) in brain tissue. Results: Compared with the control group, the 2.5, 4.5, and 8.5 mg/kg exposure groups had a significant reduction in the activity of AChE in brain tissue, and the 8.5 mg/kg exposure group had a significant increase in the level of ACh in liver tissue (P<0.05) . Compared with the control group, the 4.5 and 8.5 mg/kg exposure groups had a significant increase in the level of MDA and significant reductions in the level of GSH and the activities of SOD and GSH-Px in brain tissue (P<0.05) . Compared with the control group, the 2.5, 4.5, and 8.5 mg/kg exposure groups had significant increases in the levels of TNF-α and IL-6 in brain tissue, and the 4.5 and 8.5 mg/kg exposure groups had a significant increase in the level of IL-1ß (P<0.05) . Conclusion: Crotonaldehyde exposure can induce nervous system injury in rats, possibly by altering oxidative balance and upregulating the expression of inflammatory factors in brain tissue.


Asunto(s)
Aldehídos , Estrés Oxidativo , Animales , Glutatión Peroxidasa/metabolismo , Masculino , Malondialdehído , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo
14.
Med Sci Monit ; 27: e926492, 2021 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-33563887

RESUMEN

BACKGROUND The aim of this study was to evaluate the potential role of dual oxidase 1 (DUOX1) in wound healing. MATERIAL AND METHODS Primary fibroblasts were isolated from wound granulation tissue. Fibroblasts cell lines were established using DUOX1 overexpression and interference. Cell proliferation and reactive oxygen species (ROS) production were measured and compared among the groups. RESULTS DUOX1 expression was highest in the slow-healing tissues (P<0.05). Knockdown of DUOX1 significantly increased cell proliferation and inhibited ROS production and cell apoptosis (P<0.01). Moreover, expression of malondialdehyde (MDA) was significantly reduced, while expression of superoxide dismutase (SOD) expression was significantly increased (P<0.01). In addition, DUOX1 silencing significantly upregulated collagen I, collagen III, and NF-kappaB protein levels in the cytoplasm, and inhibited the protein levels of P21, P16, and NF-kappaB in the nucleus (P<0.01). Overexpression of DUOX1 caused a reverse reaction mediated by knockdown of DUOX1. When DUOX1-overexpressing cells were treated with the ROS inhibitor N-acetyl-L-cysteine (NAC), the protein levels that were increased by DUOX1 overexpression were reversed. CONCLUSIONS These results suggest that knockdown of DUOX1 significantly benefits wound healing, likely by the regulation of oxidative stress via NF-kappaB pathway activation.


Asunto(s)
Oxidasas Duales/metabolismo , FN-kappa B/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Cicatrización de Heridas/fisiología , Acetilcisteína/farmacología , Adulto , Apoptosis/efectos de los fármacos , Apoptosis/fisiología , Proliferación Celular/efectos de los fármacos , Proliferación Celular/fisiología , Células Cultivadas , Oxidasas Duales/genética , Femenino , Fibroblastos , Técnicas de Silenciamiento del Gen , Humanos , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Cultivo Primario de Células , Especies Reactivas de Oxígeno/antagonistas & inhibidores , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Superóxido Dismutasa/metabolismo , Cicatrización de Heridas/efectos de los fármacos
15.
Plant Physiol Biochem ; 160: 386-396, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33556754

RESUMEN

Reactive oxygen species (ROS) such as hydrogen peroxide at low concentrations act as signaling of several abiotic stresses. Overproduction of hydrogen peroxide causes the oxidation of plant cell lipid phosphate layer promoting senescence and cell death. To mitigate the effect of ROS, plants develop antioxidant defense mechanisms (superoxide dismutase, catalase, guaiacol peroxidase), ascorbate-glutathione cycle enzymes (ASA-GSH) (ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase), which have the function of removing and transforming ROS into non-toxic substances to maintain cellular homeostasis. Foliar or soil application of fertilizers containing B, Cu, Fe, Mn, Mo, Ni, Se and Zn at low concentrations has the ability to elicit and activate antioxidative enzymes, non-oxidizing metabolism, as well as sugar metabolism to mitigate damage by oxidative stress. Plants treated with micronutrients show higher tolerance to abiotic stress and better nutritional status. In this review, we summarized results indicating micronutrient actions in order to reduce ROS resulting the increase of photosynthetic capacity of plants for greater crop yield. This meta-analysis provides information on the mechanism of action of micronutrients in combating ROS, which can make plants more tolerant to several types of abiotic stress such as extreme temperatures, salinity, heavy metals and excess light.


Asunto(s)
Fertilizantes , Micronutrientes , Plantas , Especies Reactivas de Oxígeno/metabolismo , Estrés Fisiológico , Antioxidantes/metabolismo , Ascorbato Peroxidasas/metabolismo , Glutatión/metabolismo , Estrés Oxidativo , Superóxido Dismutasa/metabolismo
16.
J Plant Res ; 134(2): 237-248, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33591473

RESUMEN

Flooding stress nowadays is one of the major stressors for plants under climate change. This kind of stress may cause severe depression of the plant's growth through inhibition of photosynthesis and oxidative cell damage as well as changes in cell respiration. The present work aimed to study the effect of flooding stress on oxidative and antioxidative parameters in leaves of two maize hybrids (ZP 555 and ZP 606). Leaves of maize plants at the stage of three fully developed leaves were harvested after 6, 24, 72, and 144 h of applied flooding stress. Leaves were used for determination of physiological (the content of photosynthetic pigments and soluble proteins), oxidative stress parameters (the content of malondialdehyde (MDA) and H2O2) as well as antioxidants (the total polyphenols content, and activity of antioxidative enzymes [catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1), and Class III peroxidases (POX, EC, 1.11.1.7)]). Results indicated that flooding stress-induced time-dependent changes of measured parameters and those hybrids differ in response to stress. The noticeable difference between hybrids was detected in the H2O2 and MDA content. An increase in the activity of SOD, POX and polyphenols content, with the most pronounced changes in POX activity and polyphenols concentration, could minimize the cellular damage caused by flooding. The results of the present study suggest that a more robust antioxidative metabolism is essential under flooding stress and could be a protective strategy against oxidative damage induced by flooding in ZP 606 maize plants compared to ZP 555 plants.


Asunto(s)
Antioxidantes , Zea mays , Ascorbato Peroxidasas/metabolismo , Catalasa/metabolismo , Peróxido de Hidrógeno , Peroxidación de Lípido , Estrés Oxidativo , Hojas de la Planta/metabolismo , Superóxido Dismutasa/metabolismo , Zea mays/metabolismo
17.
Ecotoxicol Environ Saf ; 213: 111987, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33582408

RESUMEN

Protective effects of estrogen (E2) on traumatic brain injury (TBI) have been determined. In this study, the hepatoprotective effects of E2 after TBI through its receptors and oxidative stress regulation have been evaluated. Diffuse TBI induced by the Marmarou method in male rats. G15, PHTPP, MPP, and ICI182-780 as selective antagonists of E2 were injected before TBI. The results indicated that TBI induces a significant increase in liver enzymes [Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Glutamyl transferase (GGT)], and oxidants levels [Malondialdehyde (MDA), Nitric oxide (NO)] and decreases in antioxidant biomarkers [Glutathione peroxidase (GPx) and Superoxide dismutase (SOD)] in the brain and liver, and plasma. We also found that E2 significantly preserved levels of these biomarkers and enzymatic activity. All antagonists inhibited the effects of E2 on increasing SOD and GPx. Also, the effects of E2 on brain MDA levels were inhibited by all antagonists, but in the liver, only ICI + G15 + E2 + TBI group was affected. The impacts of E2 on brain and liver and plasma NO levels were inhibited by all antagonists. The current findings demonstrated that E2 probably improved liver injury after TBI by modulating oxidative stress. Also, both classic (ERß, ERα) and non-classic [G protein-coupled estrogen receptor (GPER)] receptors are affected in the protective effects of E2.


Asunto(s)
Estradiol/farmacología , Sustancias Protectoras/farmacología , Alanina Transaminasa/metabolismo , Animales , Antioxidantes/metabolismo , Aspartato Aminotransferasas/metabolismo , Lesiones Traumáticas del Encéfalo/metabolismo , Estradiol/metabolismo , Receptor alfa de Estrógeno/metabolismo , Receptor beta de Estrógeno/metabolismo , Estrógenos/metabolismo , Glutatión Peroxidasa/metabolismo , Hígado/efectos de los fármacos , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Ratas , Receptores Estrogénicos/metabolismo , Superóxido Dismutasa/metabolismo
18.
Ecotoxicol Environ Saf ; 208: 111575, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396101

RESUMEN

Microplastics (MPs) have aroused widespread concern due to their extensive distribution in aquatic environments and adverse effects on aquatic organisms. However, the underlying toxicity of different kinds of MPs on freshwater microalgae has not been examined in detail. In this study, we investigated the effects of polyvinyl chloride (PVC), polystyrene (PS) and polyethylene (PE) MPs on the growth of Microcystis aeruginosa, as well as on its toxin production and oxidative stress. We found that all three kinds of MPs had an obvious inhibition effect on the growth of M. aeruginosa. Considering the results of antioxidant-related indicators, the activity of superoxide dismutase (SOD) and catalase (CAT), and cell membrane integrity were greatly affected with exposure to PVC, PS and PE MPs. Moreover, the content of intracellular (intra-) and extracellular (extra-) microcystins (MCs) had a noticeable increase due to the presence of PVC, PS, and PE MPs. Finally, according to the comprehensive stress resistance indicators, the resistance of M. aeruginosa to three MPs followed the order: PE (3.701)> PS (3.607)> PVC (2.901). Our results provide insights into the effects of different kinds of MPs on freshwater algae and provide valuable data for risk assessment of different types of MPs.


Asunto(s)
Microcystis/fisiología , Microplásticos/toxicidad , Contaminantes Químicos del Agua/toxicidad , Antioxidantes/metabolismo , Catalasa/metabolismo , Agua Dulce , Microalgas/efectos de los fármacos , Microcistinas , Microcystis/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Plásticos/toxicidad , Polietileno , Poliestirenos/toxicidad , Cloruro de Polivinilo/toxicidad , Superóxido Dismutasa/metabolismo
19.
Ecotoxicol Environ Saf ; 208: 111588, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396111

RESUMEN

Rare earth elements (REEs) have been recently identified as emergent contaminants because of their numerous and increasing applications in technology. The impact of REEs on downstream ecosystems, notably aquatic organisms, is of particular concern, but has to date been largely overlooked. The purpose of this study was thus to evaluate the toxicity of lanthanide metals, lutetium (Lu) and dysprosium (Dy) in rainbow trout after 96 h of exposure. The lethal concentration (LC50) was determined and the expression of 14 genes involved in different pathways such as oxidative stress, xenobiotic detoxification, mitochondrial respiration, DNA repair, protein folding and turnover, inflammation, calcium binding and ammonia metabolism were quantified in surviving fish. In parallel, lipid peroxidation (LPO), DNA damage (DSB), metallothionein level (MT) and cyclooxygenase activity (COX) were examined. The acute 96 h-LC50 data revealed that Lu was more toxic than Dy (1.9 and 11.0 mg/L, respectively) and was able to affect all investigated pathways by changing the expression of the studied genes, to the exception of superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST). It also induced a decrease in DNA repair at concentrations 29 times below the LC50. This suggests that Lu could trigger a general stress to disrupt the cell homeostasis leading to genotoxicity without promoting oxidative stress. However, Dy induced modulation in the expression of genes involved in the protection against oxidative stress, detoxification, mitochondrial respiration, immunomodulation, protein turnover and an increase in the DNA strand breaks at concentrations 170 times lower than LC50. Changes in mRNA level transcripts could represent an early signal to prevent against toxicity of Dy, which exhibited inflammatory and genotoxic effects. This study thus provides useful knowledge enhancing our understanding of survival strategies developed by rainbow trout to cope with the presence of lanthanides in the environment.


Asunto(s)
Disprosio/toxicidad , Lutecio/toxicidad , Oncorhynchus mykiss/fisiología , Contaminantes Químicos del Agua/toxicidad , Animales , Catalasa/metabolismo , Daño del ADN , Reparación del ADN , Disprosio/metabolismo , Ecosistema , Glutatión Transferasa/metabolismo , Dosificación Letal Mediana , Peroxidación de Lípido/efectos de los fármacos , Lutecio/metabolismo , Metalotioneína/metabolismo , Metales de Tierras Raras , Oncorhynchus mykiss/metabolismo , Estrés Oxidativo/efectos de los fármacos , Superóxido Dismutasa/metabolismo
20.
Ecotoxicol Environ Saf ; 208: 111591, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33396114

RESUMEN

The Palaemonid shrimp Palaemon macrodactylus is widely distributed in coastal areas and estuaries which are easily contaminated by various pollutants. However, the responses of this species to environmental toxicants are not well described. In the present study, adult individuals of P. macrodactylus were exposed to gradient concentrations of Cadmium (Cd) to evaluate its acute toxic effects, including bioaccumulation, induced oxidative stress and changed energy metabolism in this species. The medium lethal concentration (LC50) of Cd at 24 h, 48 h, 72 h, and 96 h were 2.60, 0.88, 0.49 and 0.37 mg/L, respectively. Cd bioaccumulations in tissues of shrimp increased in a concentration-dependent manner, and higher concentration (50% 96 h-LC50, 0.185 mg/L) of Cd exposure led to a maximum increase of Cd concentration by 14.8, 145.5 and 15.8 folds in gill, hepatopancreas and abdominal muscle. Cd exposure caused a significant inhibition on the activity of catalase (CAT), and total superoxide dismutase (T-SOD), decrease in the total antioxidant capacity (T-AOC), and an increase of malonadehyde (MDA) content, which indicated a damage to the antioxidant system of shrimp. Meanwhile, Cd exposure also led to a significant up-regulation in the expression level of metallothionein gene (MT), and down-regulations at the mRNA level of heat shock protein 70 (HSP70) and CAT. Moreover, Cd exposure significantly inhibited the oxygen consumption rate (22%), and increased the ammonia excretion rate (43%), hence lead to a significant decrease of the O:N ratio (45%) in shrimp. The results indicated that Cd exposure could induce obvious oxidative stress, energy metabolic dysfunction and bioaccumulation of Cd in P. macrodactylus. The data obtained from the present study would provide useful information for further understanding on the toxicological mechanism of Cd to crustaceans in coastal areas and estuaries.


Asunto(s)
Cadmio/toxicidad , Palaemonidae/fisiología , Contaminantes Químicos del Agua/toxicidad , Animales , Antioxidantes/metabolismo , Bioacumulación , Catalasa/metabolismo , Estuarios , Branquias/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Hepatopáncreas/metabolismo , Metalotioneína/metabolismo , Estrés Oxidativo/efectos de los fármacos , Palaemonidae/metabolismo , Superóxido Dismutasa/metabolismo
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