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1.
Nat Commun ; 12(1): 2399, 2021 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-33893292

RESUMEN

Energy autonomy and conformability are essential elements in the next generation of wearable and flexible electronics for healthcare, robotics and cyber-physical systems. This study presents ferroelectric polymer transducers and organic diodes for imperceptible sensing and energy harvesting systems, which are integrated on ultrathin (1-µm) substrates, thus imparting them with excellent flexibility. Simulations show that the sensitivity of ultraflexible ferroelectric polymer transducers is strongly enhanced by using an ultrathin substrate, which allows the mounting on 3D-shaped objects and the stacking in multiple layers. Indeed, ultraflexible ferroelectric polymer transducers have improved sensitivity to strain and pressure, fast response and excellent mechanical stability, thus forming imperceptible wireless e-health patches for precise pulse and blood pressure monitoring. For harvesting biomechanical energy, the transducers are combined with rectifiers based on ultraflexible organic diodes thus comprising an imperceptible, 2.5-µm thin, energy harvesting device with an excellent peak power density of 3 mW·cm-3.


Asunto(s)
Fuentes de Energía Bioeléctrica , Técnicas Biosensibles/instrumentación , Electrónica Médica/instrumentación , Transductores , Dispositivos Electrónicos Vestibles , Técnicas Biosensibles/métodos , Electrónica Médica/métodos , Humanos , Sistemas Microelectromecánicos/instrumentación , Sistemas Microelectromecánicos/métodos , Monitoreo Fisiológico/instrumentación , Monitoreo Fisiológico/métodos , Reproducibilidad de los Resultados , Robótica/instrumentación , Robótica/métodos
2.
Biosensors (Basel) ; 11(4)2021 Apr 07.
Artículo en Inglés | MEDLINE | ID: mdl-33917183

RESUMEN

This review summarizes the state of the art of paper-based biosensors (PBBs) for coronavirus disease 2019 (COVID-19) detection. Three categories of PBB are currently being been used for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostics, namely for viral gene, viral antigen and antibody detection. The characteristics, the analytical performance, the advantages and drawbacks of each type of biosensor are highlighted and compared with traditional methods. It is hoped that this review will be useful for scientists for the development of novel PBB platforms with enhanced performance for helping to contain the COVID-19 outbreak, by allowing early diagnosis at the point of care (POC).


Asunto(s)
Técnicas Biosensibles/métodos , Papel , Antígenos Virales/análisis , Técnicas Biosensibles/instrumentación , Sistemas CRISPR-Cas/genética , Humanos , Inmunoensayo , Sistemas de Atención de Punto , ARN Viral/análisis , /aislamiento & purificación , /metabolismo
3.
Biosensors (Basel) ; 11(4)2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33918325

RESUMEN

Field-effect transistor (FET) biosensors have been intensively researched toward label-free biomolecule sensing for different disease screening applications. High sensitivity, incredible miniaturization capability, promising extremely low minimum limit of detection (LoD) at the molecular level, integration with complementary metal oxide semiconductor (CMOS) technology and last but not least label-free operation were amongst the predominant motives for highlighting these sensors in the biosensor community. Although there are various diseases targeted by FET sensors for detection, infectious diseases are still the most demanding sector that needs higher precision in detection and integration for the realization of the diagnosis at the point of care (PoC). The COVID-19 pandemic, nevertheless, was an example of the escalated situation in terms of worldwide desperate need for fast, specific and reliable home test PoC devices for the timely screening of huge numbers of people to restrict the disease from further spread. This need spawned a wave of innovative approaches for early detection of COVID-19 antibodies in human swab or blood amongst which the FET biosensing gained much more attention due to their extraordinary LoD down to femtomolar (fM) with the comparatively faster response time. As the FET sensors are promising novel PoC devices with application in early diagnosis of various diseases and especially infectious diseases, in this research, we have reviewed the recent progress on developing FET sensors for infectious diseases diagnosis accompanied with a thorough discussion on the structure of Chem/BioFET sensors and the readout circuitry for output signal processing. This approach would help engineers and biologists to gain enough knowledge to initiate their design for accelerated innovations in response to the need for more efficient management of infectious diseases like COVID-19.


Asunto(s)
Técnicas Biosensibles/métodos , Enfermedades Transmisibles/diagnóstico , Transistores Electrónicos , Técnicas Biosensibles/instrumentación , /virología , Enfermedades Transmisibles/virología , Humanos , Nanocables/química , Sistemas de Atención de Punto , ARN Viral/análisis , /aislamiento & purificación , Relación Señal-Ruido
4.
Biosensors (Basel) ; 11(4)2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33921010

RESUMEN

The rapid spread of epidemic diseases (i.e., coronavirus disease 2019 (COVID-19)) has contributed to focus global attention on the diagnosis of medical conditions by ultrasensitive detection methods. To overcome this challenge, increasing efforts have been driven towards the development of single-molecule analytical platforms. In this context, recent progress in plasmonic biosensing has enabled the design of novel detection strategies capable of targeting individual molecules while evaluating their binding affinity and biological interactions. This review compiles the latest advances in plasmonic technologies for monitoring clinically relevant biomarkers at the single-molecule level. Functional applications are discussed according to plasmonic sensing modes based on either nanoapertures or nanoparticle approaches. A special focus was devoted to new analytical developments involving a wide variety of analytes (e.g., proteins, living cells, nucleic acids and viruses). The utility of plasmonic-based single-molecule analysis for personalized medicine, considering technological limitations and future prospects, is also overviewed.


Asunto(s)
Técnicas Biosensibles/métodos , Virosis/diagnóstico , Biomarcadores/análisis , Biomarcadores/metabolismo , Técnicas Biosensibles/instrumentación , /virología , Humanos , Nanopartículas/química , Ácidos Nucleicos/análisis , /aislamiento & purificación , Análisis de la Célula Individual , Resonancia por Plasmón de Superficie , Virosis/virología
5.
Biosensors (Basel) ; 11(4)2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33921114

RESUMEN

Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic acid amplification have been suggested as gold standards for identifying different species, these methods are not suitable for the rapid diagnosis of pathogens owing to their long result acquisition times and complexity. In this study, we developed a rapid bio-optical sensor that uses a ball-lensed optical fiber (BLOF) probe and an automatic analysis platform to precisely diagnose infectious pathogens. The BLOF probe is easy to align and has a high optical sensing sensitivity (1.5-fold) and a large detection range (1.2-fold) for an automatic optical sensing system. Automatic signal processing of up to 250 copies/reaction of DNA of Q-fever-causing Coxiella burnetii was achieved within 8 min. The clinical utility of this system was demonstrated with 18 clinical specimens (9 Q-fever and 9 other febrile disease samples) by measuring the resonant wavelength shift of positive or negative samples for Coxiella burnetii DNA. The results from the system revealed the stable and automatic optical signal measurement of DNA with 100% accuracy. We envision that this BLOF probe-based sensor would be a practical tool for the rapid, simple, and sensitive diagnosis of emerging infectious pathogens.


Asunto(s)
Técnicas Biosensibles/métodos , ADN Bacteriano/análisis , Fibras Ópticas , Fiebre Q/diagnóstico , Automatización , Técnicas Biosensibles/instrumentación , Coxiella burnetii/genética , Coxiella burnetii/aislamiento & purificación , Humanos , Fiebre Q/microbiología , Procesamiento de Señales Asistido por Computador
6.
Food Chem ; 354: 129578, 2021 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-33756331

RESUMEN

A microfluidic colorimetric biosensor was developed using thiolated polystyrene microspheres (SH-PSs) for aggregating of gold nanoparticles (AuNPs), a novel hose-based microvalve for controlling the flow direction, and a smartphone imaging APP for monitoring colorimetric signals. Aptamer-PS-cysteamine conjugates were used as detection probes and reacted with Salmonella in samples. Complementary DNA - magnetic nanoparticle (cDNA - MNP) conjugates were used as capture probes, reacted with the free aptamer-PS-cysteamine conjugates. AuNPs were aggregated on the surface of Salmonella-aptamer-PS-cysteamine conjugates, resulting in a visible color change in the detection chamber, which indicating different concentrations of Salmonella. The limit of detection was low to 6.0 × 101 cfu/mL. The microfluidic biosensor exhibited a good specificity. It was evaluated by analyzing salad samples spiked with Salmonella. The recoveries ranged from 91.68% to 113.76%, which indicated its potential application in real samples.


Asunto(s)
Técnicas Biosensibles/instrumentación , Colorimetría/instrumentación , Dispositivos Laboratorio en un Chip , Poliestirenos/química , Salmonella/aislamiento & purificación , Teléfono Inteligente , Verduras/microbiología , Oro/química , Límite de Detección , Nanopartículas del Metal , Microesferas
7.
Nat Commun ; 12(1): 1823, 2021 03 23.
Artículo en Inglés | MEDLINE | ID: mdl-33758197

RESUMEN

The body naturally and continuously secretes sweat for thermoregulation during sedentary and routine activities at rates that can reflect underlying health conditions, including nerve damage, autonomic and metabolic disorders, and chronic stress. However, low secretion rates and evaporation pose challenges for collecting resting thermoregulatory sweat for non-invasive analysis of body physiology. Here we present wearable patches for continuous sweat monitoring at rest, using microfluidics to combat evaporation and enable selective monitoring of secretion rate. We integrate hydrophilic fillers for rapid sweat uptake into the sensing channel, reducing required sweat accumulation time towards real-time measurement. Along with sweat rate sensors, we integrate electrochemical sensors for pH, Cl-, and levodopa monitoring. We demonstrate patch functionality for dynamic sweat analysis related to routine activities, stress events, hypoglycemia-induced sweating, and Parkinson's disease. By enabling sweat analysis compatible with sedentary, routine, and daily activities, these patches enable continuous, autonomous monitoring of body physiology at rest.


Asunto(s)
Técnicas Biosensibles/instrumentación , Técnicas Biosensibles/métodos , Regulación de la Temperatura Corporal/fisiología , Microfluídica/métodos , Sudor/metabolismo , Sudoración/fisiología , Dispositivos Electrónicos Vestibles , Cuerpo Humano , Humanos , Concentración de Iones de Hidrógeno , Hipoglucemia/metabolismo , Levodopa/metabolismo , Microfluídica/instrumentación , Enfermedad de Parkinson/metabolismo , Descanso/fisiología , Estrés Fisiológico/fisiología , Sudor/fisiología , Caminata/fisiología
8.
Methods Mol Biol ; 2265: 265-276, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33704721

RESUMEN

Liquid biopsy has emerged as the next generation target for diagnostics and therapeutic monitoring of many diseases including cancer. Liquid biopsy offers noninvasive analysis of aberrant biomolecular changes (e.g., aberrant protein expression, DNA mutation) which can provide crucial information on disease stages and therapy responses. As a diagnostically important biomarker for melanoma, the detection of the BRAFV600E aberration at the DNA and protein level in liquid biopsies confers an attractive option. This method describes the preparation and operation of an integrated multimolecular sensor (IMMS) for simultaneous detection of the BRAFV600E aberration in both molecular forms from circulating melanoma cells in liquid biopsy. IMMS integrates specific melanoma cell capture, cell release, cell lysis, and electrochemical BRAFV600E detection on a single device. IMMS is demonstrated for a sample-to-answer workflow of plasma spiked with melanoma cells.


Asunto(s)
Técnicas Biosensibles/métodos , Inmunoensayo/métodos , Dispositivos Laboratorio en un Chip , Melanoma/metabolismo , Microfluídica/instrumentación , Microfluídica/métodos , Proteínas Proto-Oncogénicas B-raf/metabolismo , Neoplasias Cutáneas/metabolismo , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/metabolismo , Técnicas Biosensibles/instrumentación , Técnicas de Cultivo de Célula/métodos , Humanos , Inmunoensayo/instrumentación , Biopsia Líquida/métodos , Melanoma/genética , Melanoma/patología , Mutación , Células Neoplásicas Circulantes/metabolismo , Células Neoplásicas Circulantes/patología , Proteínas Proto-Oncogénicas B-raf/genética , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patología
9.
Nat Commun ; 12(1): 1973, 2021 03 30.
Artículo en Inglés | MEDLINE | ID: mdl-33785760

RESUMEN

Optical evanescent sensors can non-invasively detect unlabeled nanoscale objects in real time with unprecedented sensitivity, enabling a variety of advances in fundamental physics and biological applications. However, the intrinsic low-frequency noise therein with an approximately 1/f-shaped spectral density imposes an ultimate detection limit for monitoring many paramount processes, such as antigen-antibody reactions, cell motions and DNA hybridizations. Here, we propose and demonstrate a 1/f-noise-free optical sensor through an up-converted detection system. Experimentally, in a CMOS-compatible heterodyne interferometer, the sampling noise amplitude is suppressed by two orders of magnitude. It pushes the label-free single-nanoparticle detection limit down to the attogram level without exploiting cavity resonances, plasmonic effects, or surface charges on the analytes. Single polystyrene nanobeads and HIV-1 virus-like particles are detected as a proof-of-concept demonstration for airborne biosensing. Based on integrated waveguide arrays, our devices hold great potentials for multiplexed and rapid sensing of diverse viruses or molecules.


Asunto(s)
Técnicas Biosensibles/instrumentación , Interferometría/instrumentación , Procesamiento de Señales Asistido por Computador/instrumentación , Técnicas Biosensibles/métodos , Células HEK293 , Humanos , Interferometría/métodos , Límite de Detección , Nanopartículas/química , Nanotecnología/métodos
10.
Chem Commun (Camb) ; 57(30): 3704-3707, 2021 Apr 18.
Artículo en Inglés | MEDLINE | ID: mdl-33729260

RESUMEN

Accurate and rapid diagnostic tests are critical to reducing the impact of SARS-CoV-2. This study presents early, but promising measurements of SARS-CoV-2 using the ACE2 enzyme as the recognition element to achieve clinically relevant detection. The test provides a scalable route to sensitive, specific, rapid and low cost mass testing.


Asunto(s)
/química , Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Enzimas Inmovilizadas/química , /aislamiento & purificación , /metabolismo , Técnicas Biosensibles/instrumentación , /instrumentación , Técnicas Electroquímicas/instrumentación , Electrodos , Enzimas Inmovilizadas/metabolismo , Fluorocarburos/química , Oro/química , Humanos , Límite de Detección , Glicoproteína de la Espiga del Coronavirus/metabolismo
11.
Appl Microbiol Biotechnol ; 105(7): 2615-2624, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33710356

RESUMEN

A most discussed topic of the new decade, COVID-19 is an infectious disease caused by the recently discovered SARS-CoV-2. With an exceedingly high transmission rate, COVID-19 has affected almost all the countries in the world. Absent any vaccine or specific treatment, the humanity is left with nothing but the legacy method of quarantine. However, quarantine can only be effective when combined with early diagnosis of suspected cases. With their high sensitivity and unmatched specificity, biosensors have become an area of interest for development of novel diagnostic methods. Compared to the more traditional diagnostics, nanobiotechnology introduces biosensors as different diagnostics with greater versatility in application. Today, a growing number of analytes are being accurately identified by these nanoscopic sensing machines. Several reports of validated application with real samples further strengthen this idea. As of recent, there has been a rise in the number of studies on portable biosensors. Despite the slow progression, certain devices with embedded biosensors have managed to be of diagnostic value in several countries. The perceptible increase in development of mobile platforms has revolutionized the healthcare delivery system in the new millennium. The present article reviews the most recent advancements in development of diagnostic nanobiosensors and their application in the clinical fields. KEY POINTS: • There is no specific treatment for highly transmissible SARS-CoV-2. • Early diagnosis is critical for control of pandemic. • Highly sensitive/specific nanobiosensors are emerging assets against COVID-19.


Asunto(s)
Técnicas Biosensibles/métodos , Diagnóstico Precoz , Técnicas Biosensibles/instrumentación , Humanos , Técnicas de Diagnóstico Molecular , Nanotecnología , Técnicas de Amplificación de Ácido Nucleico , /aislamiento & purificación
12.
J Vis Exp ; (169)2021 03 10.
Artículo en Inglés | MEDLINE | ID: mdl-33779607

RESUMEN

Female mosquitoes are the deadliest animals on earth, claiming the lives of more than 1 million people every year due to pathogens they transmit when acquiring a blood-meal. To locate a host to feed on, mosquitoes rely on a wide range of sensory cues, including visual, mechanical, thermal, and olfactory. The study details a technique, electroantennography (EAG), that allows researchers to assess whether the mosquitoes can detect individual chemicals and blends of chemicals in a concentration-dependent manner. When coupled with gas-chromatography (GC-EAG), this technique allows to expose the antennae to a full headspace/complex mixture and determines which chemicals present in the sample of interest, the mosquito can detect. This is applicable to host body odors as well as plant floral bouquets or other ecologically relevant odors (e.g., oviposition sites odorants). Here, we described a protocol that permits long durations of preparation responsiveness time and is applicable to both female and male mosquitoes from multiple genera, including Aedes, Culex, Anopheles, and Toxorhynchites mosquitoes. As olfaction plays a major part in mosquito-host interactions and mosquito biology in general, EAGs and GC-EAG can reveal compounds of interest for the development of new disease vector control strategies (e.g., baits). Complemented with behavioral assays, the valence (e.g., attractant, repellent) of each chemical can be determined.


Asunto(s)
Técnicas Biosensibles/instrumentación , Animales , Mosquitos Vectores
13.
Nat Commun ; 12(1): 1542, 2021 03 09.
Artículo en Inglés | MEDLINE | ID: mdl-33750816

RESUMEN

Despite the fast development of various energy harvesting and storage devices, their judicious integration into efficient, autonomous, and sustainable wearable systems has not been widely explored. Here, we introduce the concept and design principles of e-textile microgrids by demonstrating a multi-module bioenergy microgrid system. Unlike earlier hybrid wearable systems, the presented e-textile microgrid relies solely on human activity to work synergistically, harvesting biochemical and biomechanical energy using sweat-based biofuel cells and triboelectric generators, and regulating the harvested energy via supercapacitors for high-power output. Through energy budgeting, the e-textile system can efficiently power liquid crystal displays continuously or a sweat sensor-electrochromic display system in pulsed sessions, with half the booting time and triple the runtime in a 10-min exercise session. Implementing "compatible form factors, commensurate performance, and complementary functionality" design principles, the flexible, textile-based bioenergy microgrid offers attractive prospects for the design and operation of efficient, sustainable, and autonomous wearable systems.


Asunto(s)
Bioingeniería/instrumentación , Ingeniería Biomédica/instrumentación , Textiles , Dispositivos Electrónicos Vestibles , Fuentes de Energía Bioeléctrica , Fenómenos Biomecánicos , Técnicas Biosensibles/instrumentación , Humanos , Sudor
14.
Adv Mater ; 33(16): e2100218, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33683745

RESUMEN

From typical electrical appliances to thriving intelligent robots, the exchange of information between humans and machines has mainly relied on the contact sensor medium. However, this kind of contact interaction can cause severe problems, such as inevitable mechanical wear and cross-infection of bacteria or viruses between the users, especially during the COVID-19 pandemic. Therefore, revolutionary noncontact human-machine interaction (HMI) is highly desired in remote online detection and noncontact control systems. In this study, a flexible high-sensitivity humidity sensor and array are presented, fabricated by anchoring multilayer graphene (MG) into electrospun polyamide (PA) 66. The sensor works in noncontact mode for asthma detection, via monitoring the respiration rate in real time, and remote alarm systems and provides touchless interfaces in medicine delivery for bedridden patients. The physical structure of the large specific surface area and the chemical structure of the abundant water-absorbing functional groups of the PA66 nanofiber networks contribute to the high performance synergistically. This work can lead to a new era of noncontact HMI without the risk of contagiousness and provide a general and effective strategy for the development of smart electronics that require noncontact interaction.


Asunto(s)
Técnicas Biosensibles/métodos , Electrónica , Asma/diagnóstico , Materiales Biocompatibles/química , Técnicas Biosensibles/instrumentación , Electrodos , Grafito/química , Humanos , Humedad , Internet de las Cosas , Aplicaciones Móviles , Nanofibras/química , Frecuencia Respiratoria , Dispositivos Electrónicos Vestibles
15.
Carbohydr Polym ; 261: 117894, 2021 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-33766379

RESUMEN

The advent of electric skins (E-skin) with tactile sensation, flexibility, and human affinity characteristics have attracted considerable attention in extensive research fields, including intelligent robots and health monitoring, etc. To improve the intrinsic brittleness of hydrogels, a multifunctional E-skin was fabricated involving a TEMPO-NFC and a covalently cross-linked polyacrylamide (PAM) network. In this work, silver nanoparticles (AgNPs) as long-term antibacterial agent and conductive fillers were coated onto NFC nanofibers. Subsequently, this nanocomposite hydrogel was synthesized by free radical copolymerization of AM monomers with PNAg fibers as interpenetrating fibers network. Importantly with NFC present, the nanocomposite hydrogel exhibited superior mechanical performance and excellent self-recovery ability. The obtained sensor with excellent mechanical stability and sensing performance could detect mechanotransduction signal of human movements. This work provides a practicable method to prepare high antibacterial efficiency, excellent mechanical performance, and dual-modal nanocellulose-based hydrogel sensor for the broad-range application in human-motion detection and intelligence skins.


Asunto(s)
Antibacterianos , Materiales Biomiméticos/síntesis química , Técnicas Biosensibles/instrumentación , Hidrogeles , Dispositivos Electrónicos Vestibles , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/uso terapéutico , Materiales Biomiméticos/química , Celulosa/química , Conductividad Eléctrica , Humanos , Hidrogeles/síntesis química , Hidrogeles/química , Hidrogeles/uso terapéutico , Ensayo de Materiales , Mecanotransducción Celular/fisiología , Nanocompuestos/química , Nanofibras/química , Plata/química , Piel/química , Fenómenos Fisiológicos de la Piel , Estrés Mecánico , Resistencia a la Tracción
16.
Biosens Bioelectron ; 179: 113099, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33640656

RESUMEN

The SARS-CoV-2 pandemic, an ongoing global health crisis, has revealed the need for new technologies that integrate the sensitivity and specificity of RT-PCR tests with a faster time-to-detection. Here, an emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to allow for the compartmentalization of LAMP reactions, leading to faster changes in emulsion characteristics, and thus lowering time-to-detection. Within these droplets, ongoing LAMP reactions lead to adsorption of amplicons to the water-oil interface, causing a decrease in interfacial tension, resulting in smaller emulsion diameters. Changes in emulsion diameter allow for the monitoring of the reaction by use of angle-dependent light scatter (based off Mie scatter theory). Mie scatter simulations confirmed that light scatter intensity is diameter-dependent and smaller colloids have lower intensity values compared to larger colloids. Via spectrophotometers and fiber optic cables placed at 30° and 60°, light scatter intensity was monitored. Scatter intensities collected at 5 min, 30° could statistically differentiate 10, 103, and 105 copies/µL initial concentrations compared to NTC. Similarly, 5 min scatter intensities collected at 60° could statistically differentiate 105 copies/µL initial concentrations in comparison to NTC. The use of both angles during the eLAMP assay allows for distinction between high and low initial target concentrations. The efficacy of a smartphone-based platform was also tested and had a similar limit of detection and assay time of less than 10 min. Furthermore, fluorescence-labeled primers were used to validate target nucleic acid amplification. Compared to existing LAMP assays for SARS-CoV-2 detection, these times-to-detections are very rapid.


Asunto(s)
/instrumentación , Dispersión Dinámica de Luz/instrumentación , Emulsiones/química , Técnicas de Diagnóstico Molecular/instrumentación , Técnicas de Amplificación de Ácido Nucleico/instrumentación , /aislamiento & purificación , Técnicas Biosensibles/economía , Técnicas Biosensibles/instrumentación , Técnicas Biosensibles/métodos , /métodos , Dispersión Dinámica de Luz/economía , Dispersión Dinámica de Luz/métodos , Diseño de Equipo , Humanos , Límite de Detección , Técnicas de Diagnóstico Molecular/economía , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificación de Ácido Nucleico/economía , Técnicas de Amplificación de Ácido Nucleico/métodos , Teléfono Inteligente , Factores de Tiempo
17.
Biosens Bioelectron ; 179: 113074, 2021 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-33596516

RESUMEN

On global scale, the current situation of pandemic is symptomatic of increased incidences of contagious diseases caused by pathogens. The faster spread of these diseases, in a moderately short timeframe, is threatening the overall population wellbeing and conceivably the economy. The inadequacy of conventional diagnostic tools in terms of time consuming and complex laboratory-based diagnosis process is a major challenge to medical care. In present era, the development of point-of-care testing (POCT) is in demand for fast detection of infectious diseases along with "on-site" results that are helpful in timely and early action for better treatment. In addition, POCT devices also play a crucial role in preventing the transmission of infectious diseases by offering real-time testing and lab quality microbial diagnosis within minutes. Timely diagnosis and further treatment optimization facilitate the containment of outbreaks of infectious diseases. Presently, efforts are being made to support such POCT by the technological development in the field of internet of medical things (IoMT). The IoMT offers wireless-based operation and connectivity of POCT devices with health expert and medical centre. In this review, the recently developed POC diagnostics integrated or future possibilities of integration with IoMT are discussed with focus on emerging and re-emerging infectious diseases like malaria, dengue fever, influenza A (H1N1), human papilloma virus (HPV), Ebola virus disease (EVD), Zika virus (ZIKV), and coronavirus (COVID-19). The IoMT-assisted POCT systems are capable enough to fill the gap between bioinformatics generation, big rapid analytics, and clinical validation. An optimized IoMT-assisted POCT will be useful in understanding the diseases progression, treatment decision, and evaluation of efficacy of prescribed therapy.


Asunto(s)
Técnicas Biosensibles/instrumentación , Enfermedades Transmisibles/diagnóstico , Internet de las Cosas , Pruebas en el Punto de Atención , Animales , Inteligencia Artificial , Técnicas Biosensibles/métodos , Infecciones por Coronavirus/diagnóstico , Dengue/diagnóstico , Diseño de Equipo , Infecciones por VIH/diagnóstico , Fiebre Hemorrágica Ebola/diagnóstico , Humanos , Gripe Humana/diagnóstico , Malaria/diagnóstico , Infecciones por Orthomyxoviridae/diagnóstico , Infección por el Virus Zika/diagnóstico
18.
Biosens Bioelectron ; 178: 113049, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33540323

RESUMEN

Prompt diagnosis, patient isolation, and contact tracing are key measures to contain the coronavirus disease 2019 (COVID-19). Molecular tests are the current gold standard for COVID-19 detection, but are carried out at central laboratories, delaying treatment and control decisions. Here we describe a portable assay system for rapid, onsite COVID-19 diagnosis. Termed CODA (CRISPR Optical Detection of Anisotropy), the method combined isothermal nucleic acid amplification, activation of CRISPR/Cas12a, and signal generation in a single assay, eliminating extra manual steps. Importantly, signal detection was based on the ratiometric measurement of fluorescent anisotropy, which allowed CODA to achieve a high signal-to-noise ratio. For point-of-care operation, we built a compact, standalone CODA device integrating optoelectronics, an embedded heater, and a microcontroller for data processing. The developed system completed SARS-CoV-2 RNA detection within 20 min of sample loading; the limit of detection reached 3 copy/µL. When applied to clinical samples (10 confirmed COVID-19 patients; 10 controls), the rapid CODA test accurately classified COVID-19 status, in concordance with gold-standard clinical diagnostics.


Asunto(s)
Técnicas Biosensibles/métodos , /diagnóstico , Polarización de Fluorescencia/métodos , /aislamiento & purificación , Técnicas Biosensibles/instrumentación , Técnicas Biosensibles/estadística & datos numéricos , /instrumentación , Sistemas CRISPR-Cas , Diseño de Equipo , Polarización de Fluorescencia/instrumentación , Polarización de Fluorescencia/estadística & datos numéricos , Humanos , Técnicas de Diagnóstico Molecular/instrumentación , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Diagnóstico Molecular/estadística & datos numéricos , Técnicas de Amplificación de Ácido Nucleico/instrumentación , Técnicas de Amplificación de Ácido Nucleico/métodos , Técnicas de Amplificación de Ácido Nucleico/estadística & datos numéricos , Pandemias , Sistemas de Atención de Punto/estadística & datos numéricos , Procesamiento de Señales Asistido por Computador , Relación Señal-Ruido
19.
Biosens Bioelectron ; 178: 113007, 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33556807

RESUMEN

Recent progress in biosensors have quantitively expanded current capabilities in exploratory research tools, diagnostics and therapeutics. This rapid pace in sensor development has been accentuated by vast improvements in data analysis methods in the form of machine learning and artificial intelligence that, together, promise fantastic opportunities in chronic sensing of biosignals to enable preventative screening, automated diagnosis, and tools for personalized treatment strategies. At the same time, the importance of widely accessible personal monitoring has become evident by recent events such as the COVID-19 pandemic. Progress in fully integrated and chronic sensing solutions is therefore increasingly important. Chronic operation, however, is not truly possible with tethered approaches or bulky, battery-powered systems that require frequent user interaction. A solution for this integration challenge is offered by wireless and battery-free platforms that enable continuous collection of biosignals. This review summarizes current approaches to realize such device architectures and discusses their building blocks. Specifically, power supplies, wireless communication methods and compatible sensing modalities in the context of most prevalent implementations in target organ systems. Additionally, we highlight examples of current embodiments that quantitively expand sensing capabilities because of their use of wireless and battery-free architectures.


Asunto(s)
Técnicas Biosensibles/instrumentación , Tecnología Inalámbrica/instrumentación , Inteligencia Artificial , Fuentes de Energía Bioeléctrica , Técnicas Biosensibles/tendencias , Suministros de Energía Eléctrica , Fenómenos Electrofisiológicos , Diseño de Equipo , Humanos , Pandemias , Tecnología de Sensores Remotos/instrumentación , Telemetría/instrumentación , Dispositivos Electrónicos Vestibles , Tecnología Inalámbrica/tendencias
20.
Molecules ; 26(3)2021 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-33535493

RESUMEN

With the increasing prevalence of growing population, aging and chronic diseases continuously rising healthcare costs, the healthcare system is undergoing a vital transformation from the traditional hospital-centered system to an individual-centered system. Since the 20th century, wearable sensors are becoming widespread in healthcare and biomedical monitoring systems, empowering continuous measurement of critical biomarkers for monitoring of the diseased condition and health, medical diagnostics and evaluation in biological fluids like saliva, blood, and sweat. Over the past few decades, the developments have been focused on electrochemical and optical biosensors, along with advances with the non-invasive monitoring of biomarkers, bacteria and hormones, etc. Wearable devices have evolved gradually with a mix of multiplexed biosensing, microfluidic sampling and transport systems integrated with flexible materials and body attachments for improved wearability and simplicity. These wearables hold promise and are capable of a higher understanding of the correlations between analyte concentrations within the blood or non-invasive biofluids and feedback to the patient, which is significantly important in timely diagnosis, treatment, and control of medical conditions. However, cohort validation studies and performance evaluation of wearable biosensors are needed to underpin their clinical acceptance. In the present review, we discuss the importance, features, types of wearables, challenges and applications of wearable devices for biological fluids for the prevention of diseased conditions and real-time monitoring of human health. Herein, we summarize the various wearable devices that are developed for healthcare monitoring and their future potential has been discussed in detail.


Asunto(s)
Biomarcadores/análisis , Técnicas Biosensibles/instrumentación , Prestación de Atención de Salud/normas , Monitoreo Fisiológico/instrumentación , Dispositivos Electrónicos Vestibles/tendencias , Técnicas Biosensibles/tendencias , Humanos , Monitoreo Fisiológico/tendencias , Dispositivos Electrónicos Vestibles/estadística & datos numéricos
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