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2.
Plast Reconstr Surg ; 145(4): 1089-1097, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32221240

RESUMEN

Currently, fat transplantation occurs immediately after harvesting procedures. Because low rates of fat graft take are well reported in the literature, many patients require multiple surgical procedures for fat graft harvest. These subsequent procedures lead to increased cost, donor-site morbidity, and patient discomfort in the long term. The ability to preserve our patients' own adipose aspirate would allow us to counteract these shortcomings and ultimately improve the clinical outcome after fat grafting. Unfortunately, there is no optimal and practical adipose tissue cryopreservation protocol for use by the plastic surgeon at the present time. Because of this dilemma, the senior author (L.L.Q.P.) has investigated this concept in an effort to create a protocol that is both technically sound and clinically achievable to allow for the long-term preservation of adipose tissue. In this article, the authors aim to outline this effort, review current clinical applications that have been reported in the literature, and detail exciting future perspectives in the use of preserved lipoaspirates for repeated fat grafting procedures or in the form of cell-based therapy engineered for reconstructive endeavors for their patients.


Asunto(s)
Tejido Adiposo/trasplante , Contorneado Corporal/métodos , Criopreservación/métodos , Trasplante de Células Madre Mesenquimatosas/métodos , Recolección de Tejidos y Órganos/métodos , Tejido Adiposo/citología , Supervivencia de Injerto , Humanos , Trasplante Autólogo/métodos
3.
Clin Ter ; 171(2): e110-e113, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32141481

RESUMEN

Osteoradionecrosis (ORN) is a well-recognized, debilitating complication of radiotherapy for patients with head and neck cancer. There is no universally accepted treatment for ORN of the jawbones. We report on a case of refractory mandibular ORN treated by sequestrectomy of mandible and autogenous transplantation of fat graft combined with platelet-rich fibrin (PRF). Improved perfusion was observed using the laser Doppler flowmetry. This case highlights the use of autogenous free fat graft and PRF as an adjunct therapy to sequestrectomy in the management of ORN.


Asunto(s)
Tejido Adiposo/trasplante , Enfermedades Mandibulares/terapia , Osteorradionecrosis/terapia , Fibrina Rica en Plaquetas , Terapia Combinada , Humanos , Masculino , Mandíbula/cirugía , Enfermedades Mandibulares/cirugía , Persona de Mediana Edad , Factores de Riesgo , Cicatrización de Heridas
4.
Plast Reconstr Surg ; 145(3): 653-659, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32097301

RESUMEN

BACKGROUND: Autologous fat grafting is an increasingly preferred method for aesthetic facial rejuvenation. The authors' group previously described the concept of "lipotopography" as topographic surface changes that occur with fat grafting to discrete facial fat compartments. The purpose of this study was to define the "augmentation zone" of the lateral suborbicularis oculi fat compartment to understand the topographical surface changes following augmentation. METHODS: Nine cadaver hemifaces were injected with fat analogue at intervals from 1 to 4 cc. Three-dimensional photographs were taken at baseline and following each 1-cc incremental injection. The interval surface changes were calculated using three-dimensional software including perimeter, diameter, and projection. RESULTS: The augmentation zone of the lateral suborbicularis oculi fat compartment was characterized by a consistent shape and boundary. The shape was an elongated oval bound superiorly by the lid-cheek junction and inferiorly at the level of the zygomaticocutaneous ligament. Vertical and horizontal diameter and perimeter showed initial increases between 1 and 2 cc and then a plateau between 2 and 3 cc. Projection changes demonstrated an initial slow increase from 1 to 2 cc injection followed by nearly linear growth from 2 to 4 cc. CONCLUSIONS: Three-dimensional photography and computer analysis provide tools to understand the surface anatomy change in response to fat grafting specific facial fat compartments. Targeted volumization of the lateral suborbicularis oculi fat compartment also results in a unique surface change with consistent shape and anatomical boundaries. The lid-cheek junction and zygomaticocutaneous ligament were observed to restrict the expansion of fat analogue for all injection volumes. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.


Asunto(s)
Tejido Adiposo/trasplante , Técnicas Cosméticas , Cara/anatomía & histología , Imagenología Tridimensional , Rejuvenecimiento , Cadáver , Cara/diagnóstico por imagen , Cara/cirugía , Humanos , Fotograbar , Trasplante Autólogo/métodos , Resultado del Tratamiento
5.
Plast Reconstr Surg ; 145(3): 712-720, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32097312

RESUMEN

BACKGROUND: Autologous fat grafting has long been an essential technique in cosmetic and reconstructive surgery. Here, the authors report the advantages of a new device for preparing micronized fat, and they also investigated the therapeutic potential of micronized fat against ultraviolet B-induced photoaging. METHODS: Micronized fat aliquots were prepared through a connector device with trifoliate blades. The histologic structure and viability of the prepared fat samples were evaluated by calcein AM/propidium iodide staining. The levels of growth factor were measured by enzyme-linked immunosorbent assay, and flow cytometry was used to detect the ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction. The authors also evaluated the effects of micronized fat transplantation through immunohistochemistry and Masson trichrome staining in an animal model of photoaging. RESULTS: The micronized fat had a normal histologic structure and viable adipocytes. It had a higher level of hepatocyte growth factor compared with the control group, and its ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction was also higher than in the control fat preparations. Transplantation of micronized fat preparations in the animal model of photoaging produced increased skin neovascularization, epidermal cell proliferation, and dermal collagen density. CONCLUSIONS: The authors' results demonstrated that the novel device produced micronized fat easily, which can condense adipose tissue. This micronized fat was easy to use with smaller cannulas. It mitigated the signs of cutaneous photoaging and was superior to control fat. Contrary to previous reports, normal histologic structures and viable adipocytes were noted in the micronized fat.


Asunto(s)
Tejido Adiposo/trasplante , Técnicas Cosméticas , Rejuvenecimiento , Envejecimiento de la Piel/efectos de la radiación , Rayos Ultravioleta/efectos adversos , Adipocitos , Tejido Adiposo/citología , Animales , Proliferación Celular , Supervivencia Celular , Células Cultivadas , Células Epidérmicas/fisiología , Masculino , Modelos Animales , Neovascularización Fisiológica , Ratas , Piel/irrigación sanguínea , Piel/efectos de la radiación , Trasplante Autólogo/métodos
6.
Plast Reconstr Surg ; 145(3): 530e-537e, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32097306

RESUMEN

BACKGROUND: Breast augmentation with fat grafting is used as an alternative to breast implants. However, a systematic evaluation of the complication rates after fat grafting using only studies with consecutive patients has not previously been performed. In this study, the authors compiled studies reporting complication rates and radiologic changes in consecutive patients undergoing cosmetic breast augmentation with fat grafting. METHODS: Studies reporting on consecutive patients undergoing breast augmentation with fat grafting were included. Complication rates, radiologic changes, Breast Imaging Reporting and Data System assessments, and the number of patients undergoing revision surgery were extracted. Mean complication rates and radiologic changes were calculated with meta-analytical methods. RESULTS: Twenty-two studies with 2073 patients were included. The rates of major complications were low (hematoma, 0.5 percent; infection, 0.6 percent; and seroma, 0.1 percent). None of these patients needed revision surgery. The most frequent minor complication was palpable cysts in 2.0 percent of the patients; 67 percent of these were treated with aspiration. The radiologic changes in the patients after fat grafting were as follows: oil cysts, 6.5 percent; calcifications, 4.5 percent; and fat necrosis, 1.2 percent. The risk of being referred for additional radiologic imaging (e.g., to exclude malignant changes) was 16.4 percent, and the risk of being referred for biopsy was 3.2 percent. CONCLUSIONS: The complication rates after breast augmentation with fat grafting are low and support fat grafting as an alternative to breast augmentation with implants. The rates of radiologic changes are high after fat grafting, but the changes do not seem to have any therapeutic consequences for the patients.


Asunto(s)
Tejido Adiposo/trasplante , Quiste Mamario/epidemiología , Calcinosis/epidemiología , Necrosis Grasa/epidemiología , Mamoplastia/efectos adversos , Complicaciones Posoperatorias/epidemiología , Mama/diagnóstico por imagen , Mama/cirugía , Quiste Mamario/diagnóstico , Quiste Mamario/etiología , Calcinosis/diagnóstico , Calcinosis/etiología , Necrosis Grasa/diagnóstico , Necrosis Grasa/etiología , Femenino , Humanos , Mamoplastia/métodos , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/etiología , Trasplante Autólogo/efectos adversos , Trasplante Autólogo/métodos , Resultado del Tratamiento
7.
Plast Reconstr Surg ; 145(3): 827-841, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32097333

RESUMEN

BACKGROUND: Autologous fat grafting is a dynamic modality used in plastic surgery as an adjunct to improve functional and aesthetic form. However, current practices in fat grafting for soft-tissue augmentation are plagued by tremendous variability in long-term graft retention, resulting in suboptimal outcomes and repetitive procedures. This systematic review identifies and critically appraises the evidence for various enrichment strategies that can be used to augment and improve the viability of fat grafts. METHODS: A comprehensive literature search of the Medline and PubMed databases was conducted for animal and human studies published through October of 2017 with multiple search terms related to adipose graft enrichment agents encompassing growth factors, platelet-rich plasma, adipose-derived and bone marrow stem cells, gene therapy, tissue engineering, and other strategies. Data on level of evidence, techniques, complications, and outcomes were collected. RESULTS: A total of 1382 articles were identified, of which 147 met inclusion criteria. The majority of enrichment strategies demonstrated positive benefit for fat graft survival, particularly with growth factors and adipose-derived stem cell enrichment. Platelet-rich plasma and adipose-derived stem cells had the strongest evidence to support efficacy in human studies and may demonstrate a dose-dependent effect. CONCLUSIONS: Improved understanding of enrichment strategies contributing to fat graft survival can help to optimize safety and outcomes. Controlled clinical studies are lacking, and future studies should examine factors influencing graft survival through controlled clinical trials in order to establish safety and to obtain consistent outcomes.


Asunto(s)
Tejido Adiposo/trasplante , Contorneado Corporal/métodos , Supervivencia de Injerto/fisiología , Trasplante de Células Madre Mesenquimatosas/métodos , Tejido Adiposo/citología , Animales , Autoinjertos/fisiología , Contorneado Corporal/efectos adversos , Estética , Humanos , Trasplante de Células Madre Mesenquimatosas/efectos adversos , Modelos Animales , Plasma Rico en Plaquetas/fisiología , Trasplante Autólogo/efectos adversos , Trasplante Autólogo/métodos , Resultado del Tratamiento
8.
Plast Reconstr Surg ; 145(2): 445-455, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31985638

RESUMEN

BACKGROUND: Posttraumatic proximal interphalangeal (PIP) joint contractures of the digits are common and are associated with impaired hand function. However, relapse is common after surgical release of PIP joint contractures. This article presents a novel treatment strategy with a PIP joint adipofascial flap to resurface the joint after release, and compares patients with similar joint contracture release who did and did not undergo resurfacing with a PIP joint adipofascial flap. METHODS: From January of 2010 to January of 2018, 10 patients received single-digit PIP joint flexion contracture release and PIP joint adipofascial flap resurfacing; 20 patients received a stepwise release as a control group. Thirty joints were compared, and the degree of extension lag improvement over time was measured during an average follow-up period of 292.4 days. RESULTS: Greater extension lag improvement was observed in the PIP joint adipofascial flap group compared with the control group (37.0 ± 19.2 degrees versus 21.0 ± 19.5 degrees; p =0.055). The ratio of improvement was also significantly higher in the flap group (0.79 ± 0.26 versus 0.49 ± 0.46; p =0.049). Flap resurfacing appeared to have a beneficial effect on improvements in extension lag (p =0.042), whereas a higher number of secondary operations, associated fractures, and maximum visual analogue scale score 1 week postoperatively were negatively associated with extension lag in univariate analysis (p < 0.05). Generalized estimating modeling showed that flap resurfacing had a significantly positive effect on extensor lag improvement with time (ß = 2.235; p =0.04). CONCLUSIONS: PIP joint adipofascial flap resurfacing following PIP joint contracture release may improve and maintain extensor lag. Recovery of joint motion may also be quicker compared with conventional release alone. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, III.


Asunto(s)
Contractura/cirugía , Articulaciones de los Dedos/cirugía , Tejido Adiposo/trasplante , Adolescente , Adulto , Anciano , Niño , Contractura/rehabilitación , Terapia por Ejercicio/métodos , Femenino , Articulaciones de los Dedos/fisiología , Humanos , Masculino , Persona de Mediana Edad , Rango del Movimiento Articular/fisiología , Estudios Retrospectivos , Resultado del Tratamiento , Adulto Joven
10.
Medicine (Baltimore) ; 99(1): e18579, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31895805

RESUMEN

The aim of this study was to compare major voice indicators in different sub-categories, the outcome of lipoinjection for patients might be refined and some voice prognostic factors could be more particularized in specific sub-groups. This is an observational study, and sub-grouped UVFP patients into 3 categories: male vs female, BMI ≥ 24 vs BMI < 24, Age ≥ 60 vs Age < 60 for more detailed exploring whether sub-categories affected voice diagnostic and prognostic parameters. Patients' voice data is recorded before and after the autologous fat injection laryngoplasty by a multidimensional voice program. Overall, 73 patients' voice performance were improved 12 months later by vocal fold lipoinjection. In the comparison of the male with female revealed female obtained better Jita than male by surgery (Female: 174.50 ±â€Š100.58 Hz; Male: 294.82 ±â€Š253.65 Hz; P < .05). BMI ≥ 24 vs BMI < 24 showed no statistical difference. Patients aged under 60 demonstrated better Highest F0, lowest F0, NHR and ShdB than elder ones 12 months after receiving vocal fold lipoinjection. Thus, Noise-to-harmonics ratio (NHR), voice turbulence index (VTI), and ShdB (Absolute shimmer, dB) may be the major post-operative evaluating markers of patients' age under 60. Voice parameters showed no significant correlation with BMI. Female patients performed lower Jita (Absolute jitter, µsec) than male patients 1 year after receiving treatment. The experimental results in this study showed UVFP patients' gender and age may stand as significant categories on analyzing clinical voice prognostic indicators, ShdB and Jita of autologous injection laryngoplasty.


Asunto(s)
Tejido Adiposo/trasplante , Laringoplastia/métodos , Complicaciones Posoperatorias/cirugía , Parálisis de los Pliegues Vocales/cirugía , Adulto , Anciano , Femenino , Humanos , Inyecciones , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/etiología , Glándula Tiroides/cirugía , Trasplante Autólogo , Parálisis de los Pliegues Vocales/etiología , Calidad de la Voz
11.
J Surg Oncol ; 121(3): 435-440, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31943238

RESUMEN

This meta-analysis aims to determine whether autologous fat grafting (AFG) affects the risk of local-regional recurrence (LRR) in breast cancer patients. In pooled analyses of 11 eligible studies, AFG was not associated with increased LRR. Subgroup analyses showed that AFG did not increase the risk of LRR in patients undergoing breast-conserving surgery or mastectomy, in patients with in situ carcinomas or invasive carcinomas, or in patients undergoing postoperative radiotherapy.


Asunto(s)
Tejido Adiposo/trasplante , Neoplasias de la Mama/cirugía , Recurrencia Local de Neoplasia/etiología , Complicaciones Posoperatorias , Femenino , Humanos , Trasplante Autólogo
12.
Plast Reconstr Surg ; 145(1): 130-140, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31577658

RESUMEN

BACKGROUND: Platelet-rich plasma improves engraftment after fat transfer. However, the effects of platelet dose have never been investigated. The authors used magnetic resonance imaging to compare surviving graft volumes in mice after administration of four different formulations (microfat alone, and three platelet-rich plasma-enriched microfat mixes). METHODS: The authors used a random, double-blinded, fat transfer protocol using three different platelet levels: 1 million (low-dose), 500 million (medium-dose), and 1000 million (high-dose) platelets/ml, and fat alone (control). The authors grafted 0.4 ml of the 70/30 platelet-rich plasma-enriched microfat mixtures (0.4 million, 200 million, and 400 million platelets per 0.12 ml for the low-dose, medium-dose, and high-dose mixtures, respectively) or 0.4 ml of microfat alone into 22 nude mice and monitored surviving graft volumes every month for 3 months. Then, the authors histologically analyzed all grafts to assess neoangiogenesis status and fat integrity. RESULTS: Three-dimensional magnetic resonance imaging showed that the median surviving graft volumes at 3 months were 9.5 percent (interquartile range, 0 to 25 percent; p = 0.003) (high-dose), 4.1 percent (interquartile range, 0 to 18 percent; p = 0.001) (medium-dose), and 18 percent (interquartile range, 8 to 38 percent; p = 0.41) (low-dose) compared to 36 percent (interquartile range, 28 to 53 percent) for the control value. The histologic integrity of microfat-alone grafts was significantly better than those of the other grafts, although the high-dose and low-dose grafts exhibited higher levels of neoangiogenesis. CONCLUSION: Higher platelet levels in microfat grafts were associated with poor graft survival in nude mice; a clinical review would be appropriate.


Asunto(s)
Tejido Adiposo/trasplante , Supervivencia de Injerto/efectos de los fármacos , Plasma Rico en Plaquetas , Animales , Imagenología Tridimensional , Imagen por Resonancia Magnética , Ratones , Ratones Desnudos
13.
Plast Reconstr Surg ; 145(1): 151-159, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31592943

RESUMEN

BACKGROUND: This study aims to present a new therapeutic option for the treatment of thumb carpometacarpal joint osteoarthritis. Knowing that autologous fat may be beneficial for osteoarthritis through antiinflammatory and chondroprotective effects, the authors transplanted autologous adipose fat into the thumb carpometacarpal joint with the objective of postponing definite resection arthroplasty surgery. METHODS: In this pilot study, the authors performed surgery on 99 joints. The study population consisted of patients with symptomatic and radiologically confirmed osteoarthritis of the thumb carpometacarpal joint. After harvesting abdominal adipose tissue, 1 to 2 ml of fat without physical or enzymatic manipulation were transplanted into the thumb carpometacarpal joint. Surgical outcome was quantified by use of the Michigan Hand Outcomes Questionnaire in addition to strength and pain measurements during a 12-month follow-up consultation. We conducted Friedman's analysis of variance to gauge the differences over time regarding Michigan Hand Outcomes Questionnaire and pain under stress. RESULTS: From 2 weeks on, there was pain relief, both under stress and at rest. Friedman's analysis of variance revealed a significant change in pain under stress [chi-square (5) = 68.52; p < 0.001]. Postoperative Michigan Hand Outcomes Questionnaire Scores improved significantly over 12 months [chi-square (5) = 90.56; p < 0.001]. CONCLUSION: The authors' preliminary findings suggest that intraarticular autologous fat transplantation is a promising alternative treatment of carpometacarpal joint osteoarthritis of the thumb. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, IV.


Asunto(s)
Tejido Adiposo/trasplante , Artroplastia/métodos , Articulaciones Carpometacarpianas/cirugía , Osteoartritis/cirugía , Pulgar/cirugía , Adulto , Anciano , Femenino , Fuerza de la Mano , Humanos , Masculino , Persona de Mediana Edad , Dimensión del Dolor , Satisfacción del Paciente , Proyectos Piloto , Rango del Movimiento Articular , Adulto Joven
14.
Ann Otol Rhinol Laryngol ; 129(2): 201-204, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31609127

RESUMEN

OBJECTIVES: Understand the utility and technique of injection pharyngoplasty with autologous fat for the treatment of mild to moderate velopharyngeal insufficiency in adults without a history of cleft palate. METHODS: Consecutive case series of 11 patients (mean [SD] 41 ± 21 years of age) who underwent injection pharyngoplasty with autologous fat from 2012 to 2018 at a tertiary care center. Patients were followed for a mean of 8.6 ± 8 months with pre versus postoperative evaluations of improvement in rhinophonia, dysphagia, and velopharyngeal closure by nasopharyngoscopy (scored: 0, none; 1, mild; 2, moderate; 3, near-complete; 4, complete). RESULTS: Patient selection and surgical techniques are described. Mean improvements were 2.3 ± 0.86 for rhinophonia, 2.0 ± 0.89 for dysphagia, and 3.0 ± 0.95 for velopharyngeal closure by nasopharyngoscopy. Five (45%) patients underwent subsequent intervention, including four repeat fat injections and three sphincter pharyngoplasties. There were two transient complications-donor site hematoma and subjective nasal obstruction. CONCLUSIONS: Although objective assessments are lacking to date, this is among the first reports to demonstrate the effectiveness and safety of injection pharyngoplasty with autologous fat for velopharyngeal insufficiency in a population of adults without a history of cleft. We found, on average, moderate improvement in rhinophonia and dysphagia, and near-complete improvement in velopharyngeal closure by nasopharyngoscopy; however, a portion of patients required subsequent intervention.


Asunto(s)
Tejido Adiposo/trasplante , Faringe/cirugía , Insuficiencia Velofaríngea/cirugía , Adulto , Femenino , Humanos , Inyecciones , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Trasplante Autólogo , Resultado del Tratamiento , Adulto Joven
15.
J Craniofac Surg ; 31(1): 64-67, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31469732

RESUMEN

Autologous free fat graft is a widely accepted method of facial contour restoration in Parry-Romberg syndrome (PRS); however, complex and unpredictable facial anatomy can render this surgery extremely challenging. The integration of 3-dimensional (3D) technology strategies as a method to enhance surgical aesthetic outcomes has been demonstrated.A retrospective chart review was performed for PRS with autologous, free fat graft between 2016 and 2017. Based on 3D volumetric facial analysis and presurgical simulation, a 3D printed fat graft guide was produced. Surgical outcomes were analyzed by volumetric measurements of the upper, middle, and lower face segments.A total of 9 patients were included in the study. Their upper and middle facial volumes before and after the procedures showed statistically significant volume augmentation (upper face, P = 0.004; middle face, P = 0.002) 6 months postoperatively (T1). Facial asymmetry was also statistically significantly corrected (middle face P = 0.012; lower face P = 0.025) at 1 year after the procedure (T2).The advantageous application of 3D comprehensive technology for aesthetic improvements in patients with PRS with autologous, free fat graft treatment has been demonstrated. Precise preoperative planning based on simulations and postoperative 3D volumetric analyses can help adequately predict fat graft strategies.


Asunto(s)
Tejido Adiposo/trasplante , Hemiatrofia Facial/cirugía , Adolescente , Adulto , Asimetría Facial/cirugía , Femenino , Humanos , Periodo Posoperatorio , Impresión Tridimensional , Estudios Retrospectivos , Trasplante Autólogo
16.
J Craniofac Surg ; 31(1): 313-318, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31469736

RESUMEN

INTRODUCTION: Over the past decade, some studies have focused on whether platelet-rich plasma (PRP) can promote the survival of fat grafts after transplantation. However, the results of these studies have been controversial because of the variations in research methods and assessment methodologies. METHODS: In this study, the authors aspirated fat particles from the abdomen of a healthy woman, mixed them with PRP, and injected them into the backs of nude mice subcutaneously, which simulates the clinical model. The control group was designed to mix aspired fat with normal saline. The fat grafts were removed 10 weeks after transplantation, stained with hematoxylin and eosin, and sliced into pathological sections, which were subsequently scanned and analyzed using the Digital Slide Scanning System (PRECICE, Beijing, China). RESULTS: The total area of the graft section and the necrotic adipose tissue area in the control group were significantly larger than that in the PRP group. The fibrosis tissue area in the PRP group was significantly larger than that in the control group. There were no statistically significant differences in the viable adipocyte area, the cyst area, and the mature blood vessel number between the PRP and control groups. CONCLUSION: In this study, PRP did not cause an increase in the number of viable adipocytes; however, it did increase the amount of inflammation, which promoted necrotic tissue absorption and the proliferation of granulation tissue. Further research, including research on normalization of fat preparation and grafting, unification of the PRP preparation and application scheme, and optimization of histological measurement and analysis methods, is needed to assess the effect of PRP on fat grafting.


Asunto(s)
Tejido Adiposo/trasplante , Plasma Rico en Plaquetas , Adulto , Animales , Femenino , Supervivencia de Injerto , Humanos , Masculino , Ratones , Ratones Desnudos , Proyectos de Investigación , Trasplante Heterólogo
20.
Facial Plast Surg Clin North Am ; 28(1): 101-117, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31779934

RESUMEN

One of the greatest challenges in the progression of aesthetic medicine lies in providing treatments with long-term results that are also minimally invasive and safe. Keeping up with this demand are developments in autologous therapies such as adipose-derived stem cells, stromal vascular fraction, microfat, nanofat, and platelet therapies, which are being shown to deliver satisfactory results. Innovations in more traditional cosmetic therapies, such as botulinum toxin, fillers, and thread lifts, are even more at the forefront of the advancement in aesthetics. Combining autologous therapies with traditional noninvasive methods can ultimately provide patients with more effective rejuvenation options.


Asunto(s)
Técnicas Cosméticas , Procedimientos Quirúrgicos Dermatologicos , Rejuvenecimiento , Envejecimiento de la Piel , Tejido Adiposo/trasplante , Humanos , Células Madre
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