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1.
Medicine (Baltimore) ; 100(4): e24191, 2021 Jan 29.
Artículo en Inglés | MEDLINE | ID: mdl-33530211

RESUMEN

BACKGROUND: Parkinson disease (PD), the second most common neurodegenerative disease, has no cure or applicable disease-modifying approach, only symptomatic therapy. Oxidative stress and mitochondrial dysfunction play key roles in PD pathophysiology. Animal studies have demonstrated that photobiomodulation (PBM) may enhance mitochondrial function and boost adenosine triphosphate production, thus alleviating PD symptoms; however, this process can cause increased reactive oxygen species (ROS) production. Molecular hydrogen (H2) is a potent and possibly therapeutic antioxidant that can mitigate the effect of ROS. PBM targeting the brainstem may facilitate neuronal activity, and the concomitant H2 may clear additional ROS produced by PBM. Therefore, this study aimed to determine the safety and effectiveness of PBM + H2 in patients with PD. METHODS: We included 18 patients with PD (age 30-80 years) who were at Hoehn and Yahr stages II-III. All the participants received daily PBM + H2 therapy for 2 weeks. The adverse event and the Unified Parkinson Disease Rating Scale (UPDRS) scores were recorded. RESULTS: We noted that the UPDRS scores began significantly decreasing from the first week, and this improvement persisted until the end of therapy. Moreover, no adverse event was recorded. After 1 week of therapy cessation, UPDRS scores slightly increased but the improvement remained significant compared with the baseline. CONCLUSION: This novel, proof-of-concept study demonstrated that PBM+H2 therapy is safe and reduces disease severity. A larger-scaled clinical trial is warranted to completely investigate the effects of PBM + H2 therapy on PD.


Asunto(s)
Antioxidantes/uso terapéutico , Terapia por Luz de Baja Intensidad/métodos , Enfermedad de Parkinson/terapia , Agua/administración & dosificación , Agua/química , Adulto , Anciano , Anciano de 80 o más Años , Antioxidantes/administración & dosificación , Antioxidantes/efectos adversos , Terapia Combinada , Método Doble Ciego , Femenino , Humanos , Hidrógeno/química , Terapia por Luz de Baja Intensidad/efectos adversos , Masculino , Persona de Mediana Edad , Estrés Oxidativo/fisiología , Proyectos Piloto , Índice de Severidad de la Enfermedad , Agua/efectos adversos
2.
Int J Pharm ; 595: 120242, 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33484919

RESUMEN

Platelet-rich plasma (PRP) is rich in cytokines and growth factors and is a novel approach for tissue regeneration. It can be used for skin rejuvenation but the large molecular size of the actives limits its topical application. In this study, low-fluence laser-facilitated PRP was delivered to evaluate its effect on absorption through the skin, infection-induced wound, and photoaging. The PRP permeation enhancement was compared for two ablative lasers: fractional (CO2) laser and fully-ablative (Er:YAG) laser. In the Franz cell experiment, pig skin was treated with lasers with superficial ablation followed by the application of recombinant cytokines, growth factors, or PRP. The transport of interferon (IFN)-γ and tumor necrosis factor (TNF)-α was negligible in intact skin and stratum corneum (SC)-stripped skin. Both lasers significantly elevated skin deposition of IFN-γ and TNF-α from PRP, and fully-ablative laser showed a higher penetration enhancement. A similar tendency was found for vascular endothelial growth factor and epidermal growth factor. Er:YAG laser-exposed skin displayed 1.8- and 3.9-fold higher skin deposition of platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-ß1 from PRP, respectively. According to the confocal images, both laser interventions led to an extensive and deep distribution of IFN-γ and PDGF-BB in the skin. In the in vivo methicillin-resistant Staphylococcus aureus (MRSA) infection model, CO2 laser- and Er:YAG laser-assisted PRP delivery reduced bacterial load from 1.8 × 106 to 5.9 × 105 and 1.4 × 104 colony-forming units, respectively. The open wound induced by MRSA was closed by the laser-assisted PRP penetration. In the mouse photoaging model, elastin and collagen deposition were fully restored by combined PRP and full-ablative laser but not by PRP alone and PRP combined with fractional laser. Laser-facilitated PRP delivery even with a low fluence setting can be considered a promising strategy for treating some dermatological disorders.


Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Staphylococcus aureus Resistente a Meticilina/efectos de la radiación , Plasma Rico en Plaquetas/metabolismo , Envejecimiento de la Piel/efectos de la radiación , Enfermedades de la Piel/terapia , Piel/efectos de la radiación , Infecciones Cutáneas Estafilocócicas/terapia , Administración Cutánea , Animales , Terapia Combinada , Citocinas/farmacocinética , Humanos , Péptidos y Proteínas de Señalización Intercelular/farmacocinética , Láseres de Gas/uso terapéutico , Láseres de Estado Sólido/uso terapéutico , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Piel/diagnóstico por imagen , Piel/efectos de los fármacos , Piel/metabolismo , Absorción Cutánea/efectos de la radiación , Envejecimiento de la Piel/efectos de los fármacos , Porcinos , Cicatrización de Heridas/efectos de los fármacos , Cicatrización de Heridas/efectos de la radiación
3.
Photodiagnosis Photodyn Ther ; 33: 102196, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33515763

RESUMEN

Many oral signs and symptoms related to the novel coronavirus disease (COVID-19) have been reported; however, both prevalence and etiology are still undetermined. Since the clinical features of the oral lesions seen in COVID-19 are highly heterogeneous and the treatments differ considerably in the literature, the present study aimed to report a clinical case in which a combination of antimicrobial photodynamic therapy (aPDT) and photobiomodulation therapy (PBMT) was used for extensive lip lesions in a patient suffering from COVID-19. Within 4 days and without any systemic drug administration, after two sessions of aPDT and one session of PBMT, the lip lesions were completely healed, and the patient recovered her orofacial functions satisfactorily. According to the current case report and taking into consideration the evident lack of information about many aspects of COVID-19 infection, this combination of phototherapy modalities seems to be a promising tool for managing COVID-19-related lip lesions; however, more studies are necessary.


Asunto(s)
/patología , Labio/efectos de los fármacos , Labio/patología , Terapia por Luz de Baja Intensidad/métodos , Fotoquimioterapia/métodos , Femenino , Humanos , Azul de Metileno/uso terapéutico , Persona de Mediana Edad , Fármacos Fotosensibilizantes/uso terapéutico
4.
J Ethnopharmacol ; 264: 113365, 2021 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-32920135

RESUMEN

ETHNO-PHARMACOLOGICAL RELEVANCE: The Carapa guianensis Aubl, popularly known as andiroba, is a large tree of the Meliaceae family, commonly found in the Amazon region. The oil extracted from its seeds is recognized in traditional medicine and has high anti-inflammatory and analgesic potential, which are the basic prerequisites for a therapeutic agent that can be used in the treatment of oral mucositis (OM). Moreover, the aforementioned oil has antimicrobial, antiallergic, and parasiticidal actions and is effective in the management of cutaneous and muscular dysfunctions. AIM OF THE STUDY: To evaluate the therapeutic effects of andiroba gel (Carapa guianensis Aubl) on the symptomatology and evolution of OM in children with leukemia who underwent chemotherapy and to compare it to the effects of low power laser. MATERIALS AND METHODS: This randomized, double-blind clinical trial involved 60 patients of both genders with leukemia, with age ranging from six to twelve years. The patients were divided into two study groups: the andiroba group (n = 30) and the laser group (n = 30). The level of pain experienced by the patients was assessed using the Wong-Baker visual analog scale and the degree of severity of OM was assessed using a table, recommended by the World Health Organization, that depicts the degrees of severity of OM. The data obtained were analyzed using the Mann-Whitney test, with statistical significance indicated by a P value less than or equal to 0.05. RESULTS: A statistically significant reduction in the degree of OM was observed on the fourth, fifth, and sixth days and in the pain scores on the second, third, and fourth days in the andiroba group after the manifestation of OM, compared to the laser group. CONCLUSIONS: The use of andiroba oil effectively reduced the severity of OM and relieved pain, which resulted in a decrease in the severity of signs and symptoms in the patients in the andiroba group, compared to the laser group.


Asunto(s)
Antineoplásicos/efectos adversos , Terapia por Luz de Baja Intensidad/métodos , Meliaceae , Aceites Vegetales/uso terapéutico , Estomatitis/inducido químicamente , Estomatitis/terapia , Niño , Preescolar , Método Doble Ciego , Femenino , Humanos , Leucemia Mielomonocítica Juvenil/tratamiento farmacológico , Masculino , Dimensión del Dolor/efectos de los fármacos , Dimensión del Dolor/métodos , Aceites Vegetales/aislamiento & purificación , Estomatitis/diagnóstico , Resultado del Tratamiento
5.
Cancer Sci ; 112(2): 828-838, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33345417

RESUMEN

Near-infrared photoimmunotherapy (NIR-PIT) is a novel therapy for cancers that uses NIR light and antibody-photosensitizer (IR700) conjugates. However, it is difficult to deliver NIR light into the bile duct for cholangiocarcinoma (CCA) from the conventional extracorporeal apparatus. Thus, in this study, we developed a dedicated catheter with light emitting diodes (LEDs) that supersedes conventional external irradiation devices; we investigated the therapeutic effect of NIR-PIT for CCA using the novel catheter. The new catheter was designed to be placed in the bile duct and a temperature sensor was attached to the tip of the catheter to avoid thermal burn. An anti-epidermal growth factor receptor (EGFR) antibody, Panitumumab-IR700 conjugate or anti-human epidermal growth factor receptor type 2 (HER2) antibody, Trastuzumab-IR700 conjugate, was used with EGFR- or HER2-expressing cell lines, respectively. The in vitro efficacy of NIR-PIT was confirmed in cultured cells; the capability of the new catheter for NIR-PIT was then tested in a mouse tumor model. NIR-PIT via the developed catheter treated CCA xenografts in mice. NIR-PIT had an effect in Panitumumab-IR700 conjugate- and Trastuzumab-IR700 conjugate-treated CCA cells that depended on the receptor expression level. Tumor growth was significantly suppressed in mice treated with NIR-PIT using the novel catheter compared with controls (P < .01). NIR-PIT was an effective treatment for EGFR- and HER2-expressing CCA cells, and the novel catheter with mounted LEDs was useful for NIR-PIT of CCA.


Asunto(s)
Neoplasias de los Conductos Biliares/terapia , Colangiocarcinoma/terapia , Inmunoterapia/instrumentación , Terapia por Luz de Baja Intensidad/instrumentación , Animales , Catéteres , Línea Celular Tumoral , Femenino , Humanos , Inmunoterapia/métodos , Rayos Infrarrojos/uso terapéutico , Terapia por Luz de Baja Intensidad/métodos , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Panitumumab/farmacología , Fármacos Fotosensibilizantes/farmacología , Trastuzumab/farmacología , Ensayos Antitumor por Modelo de Xenoinjerto
6.
J Dermatolog Treat ; 32(1): 73-77, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31172821

RESUMEN

Background: It is anticipated that the combined treatments of ablative laser and human stem cell-conditioned media produce the synergistic effects.Objectives: To investigated the effects of human stem cell-conditioned media (HSCM) as a post-procedural agent after fractional CO2 laser procedure in patients with atrophic acne scars and skin pores. Method: Both cheeks of 15 subjects were treated with a fractional CO2 laser. HSCM was randomly applied to one of the resurfacing sites (T) and normal saline was applied to the other site as a control (C). During the next six days, a solution containing 80% HSCM and hyaluronic acid (HA) was applied on the treated side (T) and HA alone was applied to the control side (C). Scar volume and erythema were objectively evaluated using an Antera 3D® CS. Result: After two months, the scar volume was reduced by 23.5% (T) versus 15.0% (C) (p = .143) and the volume of the skin pores was reduced by 37.6% (T) versus 15.9% (C) (p = .006), while the erythema was increased by 2.8% (T) versus 3.1% (C) (p = .934). Atrophic scar and the skin pores in the HSCM-applied area improved by at least 15.0% after a single treatment session, suggesting better results compared with the control side.Conclusion: HSCM may augment the regenerative effects of fractional CO2 laser.


Asunto(s)
Cicatriz/radioterapia , Medios de Cultivo Condicionados/farmacología , Láseres de Gas/uso terapéutico , Piel/efectos de los fármacos , Acné Vulgar/complicaciones , Adulto , Anciano , Cicatriz/etiología , Eritema/etiología , Femenino , Humanos , Ácido Hialurónico/uso terapéutico , Láseres de Gas/efectos adversos , Terapia por Luz de Baja Intensidad/efectos adversos , Terapia por Luz de Baja Intensidad/métodos , Masculino , Persona de Mediana Edad , Piel/patología , Células Madre/citología , Células Madre/metabolismo , Resultado del Tratamiento , Adulto Joven
7.
Arch. esp. urol. (Ed. impr.) ; 73(8): 745-752, oct. 2020. tab, graf
Artículo en Inglés | IBECS | ID: ibc-197475

RESUMEN

OBJECTIVE: To assess current efficacy and safety of low power HoLEP (Holmium Laser Enucleation of the Prostate) for the treatment of obstructing and symptomatic prostatic adenomas and to identify the mechanisms supporting the related clinical advantages. METHODS: A systematic review was conducted using relevant databases (Ovid Medline, PubMed, Scopus and Web of Sciences), employing ("low power" OR "high power") AND ("HoLEP" OR "holmium laser enucleation of the prostate") as search terms. Inherent publications were selected according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Additionally, the reference lists of the selected papers were checked manually. RESULTS: We included any kind of study (n=15) dealing with low power HoLEP because of the scarcity of the results obtained with the bibliographic search. Low power HoLEP seems to be fully comparable to the traditional high power HoLEP in terms of feasibility, efficacy and safety. An additional clinical advantage of the low power approach might be the reduced incidence of postoperative dysuria, with limited intensity and duration, possibly due to the decreased amount of energy delivered to the capsular plane with a less aggressive modality, conjugated with appropriate technical enucleative choices. The physical rationale of low power HoLEP is discussed. CONCLUSIONS: Low power HoLEP is feasible, safe and effective, and might play a not exclusive role in the reduction of incidence, intensity and duration of postoperative dysuria


OBJETIVO: Determinar la eficacia y seguridad de el holmium de baja potencia en el tratamiento de adenomas prostáticos obstructivos y identificar los mecanismos de soporte relacionados con las ventajas clínicas. MÉTODOS: Una revisión sistemática utilizando las bases de datos más relevantes fue conducida (Ovid Medline, PubMed, Scopus and Web of Sciences). Se utilizaron alta y baja potencia y HOLEP como palabras de búsqueda. Las publicaciones fueron seleccionadas según PRISMA. RESULTADOS: Se incluyó cualquier estudio (n=15) relacionado con baja potencia HOLEP dados los pocos resultados obtenidos con la búsqueda. HOLEP de baja potencia para ser totalmente comparable al HOLEP tradicional de alta potencia en términos de eficacia y seguridad. Una ventaja clínica adicional del de baja potencia es que parece reducir la disuria postoperatoria con intensidad limitada y de más corta duración, probablemente debido a la menor energía recibida por la capsula prostática, así como por el uso conjugado de las técnicas enucleación correctas. El racional para el HOLEP de baja potencia se discutirá en el manuscrito. CONCLUSIONES: El HOLEP de baja potencia es seguro y efectivo y parece reducir la incidencia, intensidad y duración de la disuria postoperatoria


Asunto(s)
Humanos , Masculino , Hiperplasia Prostática/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Láseres de Estado Sólido/uso terapéutico , Tempo Operativo , Resultado del Tratamiento , Factores de Tiempo , Estándares de Referencia
8.
Medicine (Baltimore) ; 99(36): e22098, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32899089

RESUMEN

BACKGROUND: Chronic non-specific low back pain (LBP) is gradually increasing among populations worldwide and affects their activities. Recently, the Nd:YAG laser has been presented in the rehabilitation field. OBJECTIVES: This study aims to explore the short-term effects of the Nd:YAG laser on chronic non-specific LBP individuals. METHODS: Thirty-five individuals with chronic nonspecific LBP were included in the study from December 2019 to March 2020. Randomly, they were categorized to Nd:YAG group (n = 18) and sham laser as a control (n = 17) thrice weekly for a 6-week intervention. Modified Oswestry disability index (MODI), pain disability index (PDI), visual analogue scale (VAS), and lumbar flexion range of motion (ROM) have been assessed pre and post-6 weeks of the intervention. RESULTS: Significant improvements were observed in the Nd:YAG group (MODI, P < .001; PDI, P = .007; VAS, P < .001; lumbar ROM, P = .002), whereas the sham group showed no significant changes (MODI, P = .451; PDI, P = .339; VAS, P = .107; lumbar ROM, P = .296) after 6-week intervention. Between-group comparisons showed significant differences in tending toward the Nd:YAG group (MODI, P < .001; PDI, P = .046; VAS, P < .001; lumbar ROM, P = .003). CONCLUSIONS: Regarding the present study outcomes, short-term pulsed Nd:YAG laser (6 weeks) may reduce functional disabilities and pain intensity, and improve the lumbar flexion ROM in patients with chronic nonspecific LBP. Further well-designed randomized controlled studies with large sample sizes should be conducted regarding laser treatment.


Asunto(s)
Láseres de Estado Sólido/uso terapéutico , Dolor de la Región Lumbar/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Adulto , Enfermedad Crónica , Evaluación de la Discapacidad , Humanos , Láseres de Estado Sólido/efectos adversos , Persona de Mediana Edad , Dimensión del Dolor , Rendimiento Físico Funcional , Estudios Prospectivos , Rango del Movimiento Articular , Método Simple Ciego
9.
Am J Case Rep ; 21: e926779, 2020 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-32865522

RESUMEN

BACKGROUND Coronavirus disease 2019 (COVID-19) is associated with lung inflammation and cytokine storm. Photobiomodulation therapy (PBMT) is a safe, non-invasive therapy with significant anti-inflammatory effects. Adjunct PBMT has been employed in treating patients with lung conditions. Human studies and experimental models of respiratory disease suggest PBMT reduces inflammation and promotes lung healing. This is the first time supportive PBMT was used in a severe case of COVID-19 pneumonia. CASE REPORT A 57-year-old African American man with severe COVID-19 received 4 once-daily PBMT sessions by a laser scanner with pulsed 808 nm and super-pulsed 905 nm modes for 28 min. The patient was evaluated before and after treatment via radiological assessment of lung edema (RALE) by CXR, pulmonary severity indices, blood tests, oxygen requirements, and patient questionnaires. Oxygen saturation (SpO2) increased from 93-94% to 97-100%, while the oxygen requirement decreased from 2-4 L/min to 1 L/min. The RALE score improved from 8 to 5. The Pneumonia Severity Index improved from Class V (142) to Class II (67). Additional pulmonary indices (Brescia-COVID and SMART-COP) both decreased from 4 to 0. CRP normalized from 15.1 to 1.23. The patient reported substantial improvement in the Community-Acquired Pneumonia assessment tool. CONCLUSIONS This report has presented supportive PBMT in a patient with severe COVID-19 pneumonia. Respiratory indices, radiological findings, oxygen requirements, and patient outcomes improved over several days and without need for a ventilator. Future controlled clinical trials are required to evaluate the effects of PBMT on clinical outcomes in patients with COVID-19 pneumonia.


Asunto(s)
Afroamericanos , Betacoronavirus , Infecciones por Coronavirus/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Neumonía Viral/radioterapia , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/etnología , Humanos , Masculino , Persona de Mediana Edad , Pandemias , Neumonía Viral/diagnóstico , Neumonía Viral/etnología , Tomografía Computarizada por Rayos X , Estados Unidos/epidemiología
10.
PLoS One ; 15(8): e0237195, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32764819

RESUMEN

This study investigated the caries-preventive effect of 445 nm laser radiation in combination with fluoride on the prevention of white spot lesions. Previously, several studies have indicated the ability of 488 nm argon ion laser irradiation to reduce early enamel demineralization. A diode laser (445 nm) could be an alternative technology for possible caries-preventive potential. Each sample of a group of seventeen caries-free bovine teeth was treated in four different ways on four different zones of the labial surface: control/no treatment (C), laser irradiation only (L) (0.3 W, 60 s and applied dose of 90 J/cm2), amine fluoride application only (10,000 ppm and pH 3.9) (F), and amine fluoride application followed by laser irradiation (FL). After treatment, the teeth were subjected to a demineralization solution (pH 4.3 for 48 h at 37 °C) to induce subsurface lesions. After sectioning, the teeth were examined by light microscopy. Three teeth were analyzed by scanning electron microscopy (SEM). The depths of the subsurface lesions in the C, L, F, and FL groups were 103.01 (± 13.04), 96.99 (± 14.51), 42.59 (± 17.13), and 24.35 (± 11.38) µm, respectively. The pairwise group comparison showed the following results: p < 0.001 for FL versus C, FL versus L, F versus C, and F versus L, p = 0.019 for FL versus F and p = 0.930 for L versus C. The SEM micrographs support the light-microscopic examination. The results of the current study have shown that using relatively low irradiation settings of 445 nm laser on fluoridated enamel may be effective for prevention of white spot lesions.


Asunto(s)
Caries Dental/prevención & control , Fluoruros Tópicos/administración & dosificación , Terapia por Luz de Baja Intensidad/métodos , Desmineralización Dental/prevención & control , Animales , Bovinos , Terapia Combinada/métodos , Esmalte Dental/efectos de los fármacos , Esmalte Dental/efectos de la radiación , Esmalte Dental/ultraestructura , Humanos , Láseres de Semiconductores/uso terapéutico , Microscopía Electrónica de Rastreo
11.
Medicine (Baltimore) ; 99(32): e21611, 2020 Aug 07.
Artículo en Inglés | MEDLINE | ID: mdl-32769919

RESUMEN

BACKGROUND: Low back pain (LBP) affects approximately 51% to 57% of hospital nurses and nurses' aides in Europe. New high-risk groups include home- and long-term-care nurses and physiotherapists. A number of European countries are experiencing a shortage of healthcare workers. Light therapy has been shown to be an effective treatment for various musculoskeletal disorders, including lateral epicondylitis, temporomandibular joint pain, carpal tunnel syndrome, and delayed-onset muscle soreness. A systematic review and meta-analysis demonstrated that low-level laser therapy is an effective method for relieving non-specific chronic low back pain (NSCLBP). However, the efficacy of light-emitting diode (LED) therapy for NSCLBP is disputed. This study aims to evaluate the effect of LED therapy on NSCLBP. METHODS AND ANALYSIS: We conducted a prospective, double-blind, randomized placebo-controlled trial of 148 patients with NSCLBP. The patients were randomly assigned to 2 groups: intervention group, where patients received LED photobiomodulation therapy 3 times a week for 2 weeks, and the sham group, where patients had sham therapy 3 times a week for 2 weeks. Primary outcome measures included the visual analog scale for pain, lumbar active range of motion assessments, and chair-rising times. Secondary outcome measures included a multidimensional fatigue inventory, fear-avoidance beliefs questionnaire, and the Oswestry disability index. The outcome measures were assessed before therapy and 2weeks, 4 weeks, 8 weeks, 12 weeks, and 6 months after the first interventions were completed. DISCUSSION: This study is a prospective, single-center, double-blind, randomized, controlled study. This study aims to research the efficacy of a 2-week LED program for NSCLBP working nurse. Our results will be useful for patients, working nurses, nurses' aides, and other healthcare workers with chronic low back pain. TRIAL REGISTRATION NUMBER: NCT04424823.


Asunto(s)
Protocolos Clínicos , Dolor de la Región Lumbar/terapia , Terapia por Luz de Baja Intensidad/normas , Enfermeras y Enfermeros/estadística & datos numéricos , Adolescente , Adulto , Anciano , Dolor Crónico/terapia , Método Doble Ciego , Humanos , Terapia por Luz de Baja Intensidad/instrumentación , Terapia por Luz de Baja Intensidad/métodos , Persona de Mediana Edad , Manejo del Dolor/métodos , Manejo del Dolor/normas , Dimensión del Dolor/métodos , Estudios Prospectivos , Encuestas y Cuestionarios , Escala Visual Analógica
12.
Medicine (Baltimore) ; 99(29): e21181, 2020 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-32702878

RESUMEN

INTRODUCTION: Androgenetic alopecia is the most common form of hair loss in both sexes. In recent studies, low-level light therapy (LLLT) has been established as an effective treatment for alopecia. The purpose of this study was to evaluate the safety and efficacy of LLLT using a new helmet-type device for the treatment of androgenetic alopecia. METHOD: A randomized, sham device-controlled, double-blind clinical trial was conducted at 2 institutions. Sixty participants diagnosed with androgenetic alopecia aged from 19 to 65 years were recruited. LLLT was performed through a helmet-type device that emitted light with a mean output power of 2.36 mW/cm at a wavelength of 655 nm. Participants were divided into 2 groups, which respectively used the experimental device and a sham device. After tattooing at the central point of the vertex, phototrichograms at that point were obtained at 0, 8, and 16 weeks. The primary endpoint of the study was the difference in the rate of change of hair density between the test group and the control group. RESULTS: Comparing the results at baseline and week 16, the experimental group showed an increase in hair density of 41.90 hairs/cm and an increase in hair thickness of 7.50 µm, whereas the control group showed an increase of 0.72 hairs/cm and a decrease of 15.03 µm, respectively (P < .001). No adverse events or side effects occurred. CONCLUSION: LLLT showed a significant effect on increasing hair density in patients with androgenetic alopecia. LLLT could be a safe and effective treatment for androgenetic alopecia in both sexes.


Asunto(s)
Alopecia/orina , Dispositivos de Protección de la Cabeza/normas , Terapia por Luz de Baja Intensidad/normas , Adulto , Anciano , Alopecia/fisiopatología , Método Doble Ciego , Diseño de Equipo/métodos , Femenino , Dispositivos de Protección de la Cabeza/estadística & datos numéricos , Humanos , Terapia por Luz de Baja Intensidad/métodos , Terapia por Luz de Baja Intensidad/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Placebos/administración & dosificación , Resultado del Tratamiento
13.
Med. oral patol. oral cir. bucal (Internet) ; 25(4): e549-e553, jul. 2020. tab
Artículo en Inglés | IBECS | ID: ibc-196509

RESUMEN

BACKGROUND: This study aims to evaluate the effectiveness of the photobiomodulation therapy (PBMT) in the treatment of minor recurrent aphthous stomatitis (MiRAS) in children, in terms of pain relief, lesion size reduction and the parental satisfaction of the therapy. MATERIAL AND METHODS: This randomized controlled study was carried out on 60 children with clinical diagnosis of MiRAS. Patients were randomized into two groups: group A receiving laser therapy and group B receiving sham therapy (placebo). Laser therapy (diode laser, Lamba: 645 nm) was administered on day 1 (T0) for three consecutive days. Patients were evaluated also on day 4 (T1), on day 7 (T2) and on day 10 (T3). Oral aphthous lesions size was assessed through a periodontal probe to measure the diameter length (mm); pain was evaluated through the Visual Analogue Scale (VAS); parental satisfaction was assessed through a questionnaire. RESULTS: The difference in the reduction of ulcers diameters between the two groups resulted statistically significant at T1 and at T2 (p<0.05). A statistically significant difference in pain reduction between two groups was found at T1 (p<0.05). No statistically significant difference between the two groups of parents was found as concerns the parental acceptance of the procedure and the discomfort for the need of multiple appointments. CONCLUSIONS: PBMT is to be considered effective in the treatment of MiRAS in children and well-accepted by the parents of the children themselves


No disponible


Asunto(s)
Humanos , Masculino , Femenino , Niño , Estomatitis Aftosa/radioterapia , Terapia por Luz de Baja Intensidad/métodos , Satisfacción del Paciente , Resultado del Tratamiento , Escala Visual Analógica , Manejo del Dolor
14.
Acta Cir Bras ; 35(3): e202000303, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32490900

RESUMEN

PURPOSE: To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon. METHODS: Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis. RESULTS: The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen. CONCLUSION: Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.


Asunto(s)
Tendón Calcáneo/efectos de la radiación , Carotenoides/farmacología , Hidroxibutiratos/farmacología , Terapia por Luz de Baja Intensidad/métodos , Tendinopatía/radioterapia , Tenotomía/métodos , Tendón Calcáneo/efectos de los fármacos , Tendón Calcáneo/cirugía , Animales , Colágeno/farmacología , Colágeno Tipo I/análisis , Colágeno Tipo I/efectos de los fármacos , Colágeno Tipo III/análisis , Colágeno Tipo III/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Fibroblastos/química , Fibroblastos/efectos de los fármacos , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Cicatrización de Heridas/efectos de los fármacos , Cicatrización de Heridas/efectos de la radiación
15.
Life Sci ; 254: 117767, 2020 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-32407848

RESUMEN

OBJECTIVE: Heat stress shock affects the generation of free radicals and can have a harmful effect on spermatogenesis. Photobiomodulation (PBM) is very effective in andrology for treating male infertility. This research aimed at the evaluation of the impacts of PBM on spermatogenesis on the transient scrotal hyperthermia-induced oligospermia mouse model. MATERIALS AND METHODS: This experimental research divided 24 mice into the following four groups: (1) Control, (2) Scrotal hyperthermia, (3) Scrotal hyperthermia receiving laser 0.03 J/cm2 for 30 s for each testis, 35 days after induction of scrotal hyperthermia every other day for 35 days, and (4) Scrotal hyperthermia receiving laser 0.03 J/cm2 for 30 s for each testis, immediately after induction of scrotal hyperthermia every other day for 35 days. Scrotal hyperthermia was induced by water bath with 43 °C for 30 min. Then, the mice were euthanized, and their sperm samples were collected for sperm parameters analysis. Then, we took the testis samples for histopathological experimentations, serum testosterone level, reactive oxygen species (ROS), RNA extraction for the examination of IL1-α, IL6 and TNF-α genes expression as well as production and glutathione disulfide (GSH) activity. KEY FINDINGS: Our outputs indicated that PBM could largely improve the sperms parameters and stereological parameters, like spermatogonia, primary spermatocyte, round spermatid and Leydig cells together with an increasing level of the serum testosterone and GSH activity compared to the scrotal hyperthermia induced mice. In addition, it was found that the diameter of seminiferous tubules, ROS production, as well as the expression of IL1-α, IL6, and TNF-α genes significantly decreased in the treatment groups by PBM compared to the scrotal hyperthermia induced mice, but there was not a significant difference in terms of testis weight and Sertoli cells between the studied groups. SIGNIFICANCE: It could be concluded that PBM may be regarded as an alternative treatment for improving the spermatogenesis process in the scrotal hyperthermia induced mice.


Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Escroto/metabolismo , Espermatogénesis/efectos de los fármacos , Animales , Fiebre/metabolismo , Respuesta al Choque Térmico/fisiología , Calor , Hipertermia Inducida/métodos , Infertilidad Masculina/patología , Células Intersticiales del Testículo/metabolismo , Masculino , Ratones , Estrés Oxidativo/efectos de los fármacos , Escroto/patología , Células de Sertoli/metabolismo , Espermatozoides/patología , Testículo/efectos de los fármacos
16.
Medicine (Baltimore) ; 99(17): e19904, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32332670

RESUMEN

Hypotonia, particularly of the masticatory and oropharyngeal muscles, is 1 of the main characteristics of Down Syndrome (DS), resulting in impaired speech, chewing and swallowing. Moreover, the complete or partial obstruction of the airways during sleep may occur due to hypotonia of the tongue, leading to snoring and sleep disorders, such as obstructive apnea and sleep bruxism. OBJECTIVE:: Analyze salivary levels of dopamine and cortisol and muscle activity before and after treatment with low-level laser therapy administered to acupoints in children with DS. METHODS:: A randomized, controlled, clinical trial will be conducted. Individuals 4 to 17 years of age with a diagnosis of DS and possible sleep bruxism will be screened at the Integrated Health Clinic of Nove de Julho University. We will evaluate orofacial dysfunction (Nordic Orofacial Test - Screening questionnaire), Masseter muscle activity during sleep will be assessed by BiteStrip and the masticatory muscles will be evaluated by electromyography (BTS TMJOINT) head posture as well as salivary cortisol and dopamine. After the evaluations, the participants will be randomized into 2 groups: Grupo 1 - treatment with low-level laser therapy at a wavelength of 808 nm; Group 2 - sham treatment (simulated laser therapy). Treatment will be conducted twice per week for a total of 12 sessions. The data will be tabulated and treated using GraphPad Prism version 7.0. The Kolmogorov-Smirnov test will be used to determine the normality of the data. Variables that fit the Gaussian curve will be expressed as mean and standard deviation. The ANOVA 2-way will be used for comparisons between the groups, with the significance level set to 5% (P < .05).ClinicalTrials registration number: NCT04211870.


Asunto(s)
Protocolos Clínicos , Síndrome de Down/terapia , Terapia por Luz de Baja Intensidad/normas , Bruxismo del Sueño/terapia , Puntos de Acupuntura , Adolescente , Niño , Preescolar , Niños con Discapacidad , Femenino , Humanos , Terapia por Luz de Baja Intensidad/métodos , Masculino , Ensayos Clínicos Controlados Aleatorios como Asunto/métodos , Encuestas y Cuestionarios
17.
Int. j. morphol ; 38(2): 252-258, abr. 2020. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1056431

RESUMEN

The aim of this study was to evaluate the effects of mandibular advancement appliance and low level laser therapy (LLLT) with different doses on cellular hypertrophic changes in the mandibular condyle of rats. Forty-eight 8-week-old male Wistar albino rats weighing between 260 and 280 g were randomly divided into four experimental and control groups. Group I was the control group; group II was the mandibular advancement appliance group; group III was the 8 J/cm2 (0.25 W, 20 s) laser irradiation with mandibular advancement appliance group; and group IV was the 10 J/cm2 (0.25 W, 25 s) laser irradiation with mandibular advancement appliance group. Mandibular condyle cartilage and subchondral bone changes with different LLLT dose and mandibular advancement appliance were evaluated by histomorphometrical analysis. Subchondral bone fraction results showed that there were no significant differences between groups (p<0.05). The statistically significant differences found between control group and experimental groups in anterior and posterior cartilage layers thickness (p<0.05) and (p<0.01). Posterior and anterior condylar cartilage layers of rats react differentially to LLLT and mandibular advancement application. Maximum changes in condylar cartilage layers were found in 8 J/cm2 laser irradiation with mandibular appliance group.


El objetivo de este estudio fue evaluar los efectos del aparato de avance mandibular y la terapia con láser de bajo nivel (TLBN) con diferentes dosis sobre los cambios hipertróficos celulares, en el cóndilo mandibular de ratas. Cuarenta y ocho ratas albinas macho Wistar de 8 semanas de edad con un peso de 260 y 280 g se dividieron aleatoriamente en cuatro grupos experimentales y control. El grupo I control; grupo II, dispositivos de avance mandibular; grupo III de irradiación con láser de 8 J / cm2 (0.25 W, 20 s) con el grupo dispositivos de avance mandibular; y grupo IV con irradiación láser de 10 J / cm2 (0,25 W, 25 s) con el grupo de dispositivos de avance mandibular. El cartílago del cóndilo mandibular y los cambios en el hueso subcondral con diferentes dosis de TLBN y dispositivo de avance mandibular, se evaluaron mediante análisis histomorfométrico. Los resultados de la fracción ósea subcondral indicaron que no hubo diferencias significativas entre los grupos (p <0,05). Las diferencias estadísticamente significativas encontradas entre el grupo control y los grupos experimentales, en el grosor del cartílago anterior y posterior (p<0,05) y (p<0,01). Las capas de cartílago condilar posterior y anterior de las ratas reaccionan de manera diferencial a la aplicación de TLBN y avance mandibular. Se encontraron cambios significativos en las capas de cartílago condilar con irradiación láser de 8 J /cm2 con el grupo de dispositivos mandibulares.


Asunto(s)
Animales , Masculino , Ratas , Huesos/efectos de la radiación , Cartílago Articular/efectos de la radiación , Avance Mandibular/métodos , Terapia por Luz de Baja Intensidad/métodos , Huesos/cirugía , Cartílago Articular/cirugía , Ratas Wistar
18.
Medicine (Baltimore) ; 99(12): e19547, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32195961

RESUMEN

Obstructive sleep apnea (OSA) increases morbidity and mortality and it is associated with an increased cardiovascular risk. The gold standard treatment for OSA is positive airway pressure therapy (CPAP). However, it is an expensive treatment and several patients do not adapt to CPAP. GOAL: The researchers will verify the effects of low-level laser therapy (LLLT) on OSA, when applied to the soft palate and on the tongue base. METHODS: The researchers will select individuals of both sexes aged 30 to 60 years old who are sedentary and that present a high risk of OSA by the Berlin questionnaire. The evaluations pre and post interventions will be polysomnography; anthropometric and body composition measurements (Bioimpedance); metabolic syndrome risk factors (International Diabetes Federation); physical capacity (VO2 peak at the cardiopulmonary exercise test, CPET); endothelial function (flow-mediated dilatation, FMD); autonomic control (heart rate variability and sympathovagal balance). Those diagnosed with moderate and severe OSA (apnea/hypopnea index, AHI ≥15 events/h) will be invited to participate in the study and they will be randomized into 2 groups: LLLT treatment or placebo (C). The LLLT group will receive applications at 8 points on the soft palate and on the base of the tongue for 8 seconds for each point. The applications of LLLT will occur twice a week, with a minimum interval of 2 days between the applications for 2 months, when using a Therapy Plus NS 13678 Laser. The C group will have similar applications, but with the device turned off. EXPECTED RESULTS: In the individuals with OSA, photobiomodulation through LLLT will decrease the AHI. Additionally, when LLLT is applied in the oral cavity, a highly vascularized region, this may cause improvements in the vascular function and in the autonomic and hemodynamic control. ETHICS AND DISSEMINATION: This protocol was approved by the Research Ethics Committee of the Nove de Julho University, São Paulo, Brazil, on the date of March 11, 2019 (CAAE: 06025618.2.0000.5511 - Acceptance Number: 3.191.077). This trial has been registered with the Brazilian Registry of Clinical Trials (REBEC TRIAL RBR-42v548). This study is not yet recruiting. Issue date: November 4, 2019.


Asunto(s)
Terapia por Luz de Baja Intensidad/métodos , Boca/efectos de la radiación , Apnea Obstructiva del Sueño/fisiopatología , Apnea Obstructiva del Sueño/terapia , Adulto , Brasil/epidemiología , Prueba de Esfuerzo/métodos , Femenino , Humanos , Masculino , Síndrome Metabólico/epidemiología , Persona de Mediana Edad , Boca/irrigación sanguínea , Paladar Blando/efectos de la radiación , Polisomnografía/métodos , Factores de Riesgo , Apnea Obstructiva del Sueño/complicaciones , Apnea Obstructiva del Sueño/mortalidad , Lengua/efectos de la radiación
19.
Medicine (Baltimore) ; 99(13): e19430, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32221067

RESUMEN

INTRODUCTION: Loss of a dental element can generate several repercussions in the stomatognathic system. According to the latest survey by the Ministry of Health, in 2010, Brazilian adults had, on average, 7 missing teeth. This loss may lead to movement of the adjacent teeth and the antagonist, which would make prosthetic rehabilitation harder to do. Anchoring systems, such as mini-implants, have been increasingly used as a treatment option because they act with heavy but controlled forces and without side effects. Recent studies have shown that photobiomodulation (PBM) can accelerate orthodontic movement in molar intrusion. The objective of this study will be to evaluate the effect of PBM on the acceleration of the orthodontic movement of molar verticalization and its effect on pain and inflammation of the periodontal tissues. PATIENT CONCERNS:: the concerns assessments will be done over the study using anamnesis interviews and specific questionnaire. DIAGNOSIS: verticalization will be evaluated by clinical and radiographic analysis. INTERVENTIONS: Thirty four healthy patients aged 30 to 60 years, who need to recover the prosthetic space for oral rehabilitation after loss of the posterior inferior dental elements and inclination of the adjacent element, will be randomly divided into 2 groups: G1 (control group) - verticalization by mini-implant + PBM simulation (placebo); G2 (experimental group) - verticalization by mini-implant + PBM. The movements will occur with the aid of mini-implants and elastomeric chains ligatures. The PBM will occur with diode laser application, 808 nm, 100 mW, receiving 1J per point, 10 seconds, 10 points (5 per buccal and 5 per lingual) and radiant exposure of 25 J/cm. The orthodontic forces of verticalization (corresponding to any exchange of elastomeric ligation) will be applied every 30 days and the PBM will be applied immediately, 3 and 7 days of each month, for a period of 3 months. The crevicular gingival fluid (CGF) will be collected on the 1st, 3rd, and 7th days after the first activation, and then on the 3rd day of the following 2 months. OUTCOMES: Interleukins IL1ß, IL-6, IL-8, IL-10, and TNF-α will be analyzed by ELISA. Panoramic radiography will be performed at baseline and 90 afterwards to ascertain the amount (in degrees) of verticalization. To evaluate the pain, the Visual Analog Scale (VAS) will be used in all the consultations, and to evaluate the quality of life, the Oral Health Impact Profile (OHIP-14) questionnaire will be applied. Analgesics will be given and the quantity of drugs will be counted. If the data are normal, they will be submitted to Student t test. The data will be presented as means ± SD and the value of p will be defined as <0.05. DISCUSSION: This protocol will determine the effectiveness of photobiomoduation regarding the orthodontic movement of molar verticalization. ETHICS AND DISSEMINATION: This protocol received approval from the Human Research Ethics Committee of Universidade Nove de Julho (certificate number: 3 533 219). The data will be published in a peer-reviewed periodical.


Asunto(s)
Interleucinas/biosíntesis , Terapia por Luz de Baja Intensidad/métodos , Diente Molar/efectos de la radiación , Técnicas de Movimiento Dental/métodos , Adulto , Brasil , Método Doble Ciego , Femenino , Líquido del Surco Gingival , Humanos , Láseres de Semiconductores , Masculino , Persona de Mediana Edad , Dolor/etiología , Dimensión del Dolor , Calidad de Vida , Técnicas de Movimiento Dental/efectos adversos , Factor de Necrosis Tumoral alfa/biosíntesis
20.
Med. oral patol. oral cir. bucal (Internet) ; 25(2): e283-e290, mar. 2020. graf, tab, ilus
Artículo en Inglés | IBECS | ID: ibc-196258

RESUMEN

BACKGROUND: To assess if photobiostimulation (PBS) alleviates pain intensity/duration and swelling after implant surgery. MATERIAL AND METHODS: Sixty subjects (27 male and 33 female, with a mean age of 47,13 8.05 years) were included and randomly assigned to experimental group (implant surgery and photobiostimulation), placebo group (implant surgery and simulated photobiostimulation) and control group (implant surgery only). INCLUSION CRITERIA: subjects older than 20 years, with a healthy oral mucosa and requiring implant surgery. EXCLUSION CRITERIA: pregnancy, history of implant failure, light sensitivity, metabolic deseases, consumption of antibiotics or corticosteroids in the last two weeks, smokers and alcohol drinkers. Patients reported the pain experienced by using a numeric rating scale (NRS) at 2 hours, 6 hours, 12 hours, 24 hours and from day 2 to 7. Swelling score was assessed by linear measurements and type and number of analgesic drugs within each time-point were recorded on a spreadsheet. Data of pain and amount of swelling were compared among the three groups by using the Kruskal-Wallis H Test and post-hoc comparisons tests. RESULTS: Pain in the experimental group was less compared to controls and placebo group, at each time intervals (p < 0.001) as well as the maximum pain score (experimental group: median = 2, interquartile range 2-3; control group: median = 8, interquartile range 3,75-9; placebo group: median = 8, interquartile range 6,25-9). Swelling was almost insignificant in the experimental group (maximum value = 1, interquartile range 0-2,75, at 24 hours) compared with control (maximum value = 6, interquartile range 5-8,75, at 24 hours) and placebo (maximum value = 6, interquartile range 5-8, at 24 hours). Subjects in the experimental group assumed less analgesics compared to both controls and placebo groups. CONCLUSIONS: Photobiostimulation is an effective method to reduce pain intensity/duration and swelling after implant surgery


No disponible


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Implantación Dental/efectos adversos , Dolor Postoperatorio/prevención & control , Terapia por Luz de Baja Intensidad/métodos , Factores de Tiempo , Resultado del Tratamiento , Análisis de Varianza , Estadísticas no Paramétricas , Dimensión del Dolor
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