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1.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(1): 7-12, 2020 Jan.
Artículo en Chino | MEDLINE | ID: mdl-31950782

RESUMEN

Objective: To study the effects of genistein (GEN) on reproductive system in prepubertal male rats. Methods: Thirty SPF-rated male SD rats were randomly divided into control group (Con group), low-dose group (G1 group) and high-dose group (G2 group), with 10 rats in each group. Corn oil, 150 mg/kg and 300 mg/kg GEN dissolved in corn oil of equal volume were respectively administered every day and weighed the next day. After 6 weeks, the rats were sacrificed, and the testis, epididymis and prostate were dissected, and organ coefficients were calculated. Histopathological changes of testis was observed. The number of sperm was counted and the rate of sperm malformation was calculated. The concentrations of serum testosterone and estradiol were detected by radioimmunoassay. The protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C) protein expression in testicular tissue was detected by immunofluorescence assay. The mRNA and protein expression levels of PPP2R2C and cyclin dependent protein kinases 2 (CDK2) in rat testis were detected by real-time quantitative fluorescence PCR (RT-qPCR) and Western blot, respectively. The protein phosphatase 2A (PP2A) activity in testicular tissue was detected by immunoprecipitation. Results: There were no statistically significant differences in body mass, sperm number, serum estradiol and PP2A enzyme activity among the groups ( P>0.05). The pathological structure of testicular in G2 group was disordered. Sperm abnormality rate in G1 and G2 groups was higher than that in Con group ( P<0.05). Serum testosterone concentration in G2 group was lower than that in Con group ( P<0.05). The expression of PPP2R2C and CDK2 in G2 group was higher than that in Con group ( P<0.05), but the protein level was lower than that in Con group ( P<0.05). PPP2R2C protein was expressed in testicular tissue in each group. Conclusion: Long-term exposure to high dose (300 mg/kg) GEN during prepuberty may cause adverse effects on reproductive function in adult male rats. Further investigation is needed to determine whether PPP2R2C-PP2A-CDK2 phosphorylation pathway affects reproductive system in rats.


Asunto(s)
Genisteína , Genitales Masculinos , Animales , Estradiol/sangre , Regulación de la Expresión Génica/efectos de los fármacos , Genisteína/farmacología , Genitales Masculinos/efectos de los fármacos , Masculino , Fitoestrógenos/farmacología , Ratas , Ratas Sprague-Dawley , Recuento de Espermatozoides , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Testículo/enzimología , Testosterona/sangre
2.
Mymensingh Med J ; 29(1): 66-72, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31915338

RESUMEN

Various forms of sexual dysfunction occur in men with diabetes mellitus (DM) including disorders of libido, ejaculatory problems, and erectile dysfunction (ED). This cross sectional study was conducted in a tertiary hospital of Bangladesh from December 2017 to May 2018 to find out the frequency and risk factors of ED in subjects with type 2 DM (T2DM). One hundred fifty (150) consecutive male patients with T2DM attending the Endocrinology outpatient department (OPD) of the hospital during the study period were evaluated for the presence of ED by using the International Index of Erectile Function-5 (IIEF-5) questionnaire; their socio-demographic, anthropometric, and clinical data were also recorded. Glycemic status was assessed by measurement of fasting plasma glucose (FPG) and HbA1c. Morning serum testosterone was measured in all. Among 150 subjects 68(45.3%) had ED; ED was mild in 14.7%, mild to moderate in 18.0%, moderate in 6.0% whereas severe ED was present in 6.7% of the subjects. The subjects with ED had higher mean age, longer duration of DM, higher body mass index (BMI), higher HbA1c, higher FPG, higher serum creatinine, and lower serum testosterone level than those without ED. Study subjects in the higher age group and higher duration of DM had higher frequencies of ED. IIEF-5 score showed significant negative correlation with age, duration of DM, HbA1c, fasting plasma glucose, serum creatinine and significant positive correlation with serum testosterone. In logistic regression analysis, duration of DM and serum testosterone were found be independent predictors of ED. Frequency of ED among Bangladeshi type 2 diabetic males is high; duration of DM and serum testosterone are independent predictors of ED in them.


Asunto(s)
Diabetes Mellitus Tipo 2/complicaciones , Disfunción Eréctil/epidemiología , Adulto , Distribución por Edad , Bangladesh/epidemiología , Glucemia/análisis , Índice de Masa Corporal , Estudios Transversales , Diabetes Mellitus Tipo 2/epidemiología , Disfunción Eréctil/etiología , Hemoglobina A Glucada/análisis , Humanos , Masculino , Persona de Mediana Edad , Obesidad/epidemiología , Prevalencia , Factores de Riesgo , Testosterona/sangre
3.
Medicine (Baltimore) ; 99(1): e18605, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31895811

RESUMEN

To investigate the age-related nomograms and change trends of reproductive hormones, and prevalence of androgen deficiency (AD) in middle-aged and aging men from 2 studies.Two cross-sectional studies were conducted at 5-year intervals in Chinese community-dwelling men living in the same area. A total of 434 (Study 1, S1) and 944 (Study 2, S2) men aged 40 to 69 years were recruited as subjects and 59 (S1) and 98 (S2) men aged 20 to 39 years as controls to measure serum reproductive hormone levels.Serum total testosterone (TT) levels did not change significantly in S1, whereas TT levels increased in S2 with aging. Serum calculated free testosterone (cFT) levels gradually decreased with aging; however, only men aged 40 to 69 years showed this trend in S2. Serum luteinizing hormone (LH) and sex hormone binding globulin (SHBG) levels gradually increased, and serum testosterone secretion index (TSI) and free testosterone index (FTI) levels gradually decreased with male aging. The mean annual decrease values of serum cFT were 2.705 pmol/l in S1 and 1.060 pmol/l in S2. The cut-off values for AD in S1 and S2 were 9.13 nmol/l and 9.35 nmol/l for TT, and 169.00 pmol/l and 213.90 pmol/l for cFT. Using TT or cFT cut-off values, mean AD prevalence was 14.52% or 44.70% in S1, and 6.36% or 16.53% in S2. Based on cFT cut-off values, prevalence of AD increased gradually with male aging in a range of 25.30% to 61.63% in S1 and 1.20% to 23.03% in S2.The change patterns of serum LH, SHBG, TSI and FTI levels in middle-aged and aging males were consistent; however, there were differences in serum TT and cFT change patterns in S1 and S2 with male aging. cFT cut-off values were the optimal metric to evaluate AD, which can be present a ladder-like change in prevalence of different age groups.


Asunto(s)
Envejecimiento/sangre , Enfermedades del Sistema Endocrino/epidemiología , Hormona Luteinizante/sangre , Globulina de Unión a Hormona Sexual/metabolismo , Testosterona/sangre , Adulto , Anciano , China/epidemiología , Estudios Transversales , Humanos , Masculino , Persona de Mediana Edad , Nomogramas , Prevalencia , Testosterona/deficiencia , Adulto Joven
4.
J Urol ; 203(2): 398-404, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31393814

RESUMEN

PURPOSE: We examined the relationship of the serum testosterone level to low fat, Mediterranean and low carbohydrate diets in a large, nationally representative patient sample. MATERIALS AND METHODS: We queried the NHANES (National Health and Nutrition Examination Survey) from 1999 to 2000, 2003 to 2004 and 2011 to 2012. Men 18 to 80 years old who completed the 2-day dietary history and underwent serum testosterone testing were included in analysis. Diets were categorized as low fat, Mediterranean, low carbohydrate or nonrestrictive. Multivariable modeling was used to determine the relationship between diet and serum testosterone. RESULTS: Of the 3,128 men who met study inclusion criteria 457 (14.6%) and 764 (24.4%) met the criteria for a low fat and a Mediterranean diet, respectively. Only 2 men (less than 0.1%) met the criteria for a low carbohydrate diet, which was removed from further analysis. Mean ± SD serum testosterone was 435.5 ± 6.7 ng/dl. Mean testosterone was lower among men with a low fat diet (410.8 ± 8.1 vs 443.5 ± 7.3, p=0.005) and a Mediterranean diet (412.9 ± 9.1 vs 443.5 ± 7.3, p=0.002). Multivariable analysis controlling for age, body mass index, activity level, diabetes, comorbidities and prostate cancer showed that men with a nonrestrictive diet had higher serum testosterone than those adhering to a low fat diet (ß -57.2, 95% CI -105.6 to -8.8, p <0.05). CONCLUSIONS: Men adhering to low fat diets had lower serum testosterone levels even when controlling for comorbidities, age, body mass index and activity levels. As differences in serum testosterone between the diets were modest, the avoidance of fat restrictive diets should be weighed against the potential benefits on an individual basis.


Asunto(s)
Dieta Baja en Carbohidratos , Dieta con Restricción de Grasas , Dieta Mediterránea , Testosterona/sangre , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Humanos , Masculino , Persona de Mediana Edad , Estados Unidos , Adulto Joven
5.
Toxicol Lett ; 319: 1-10, 2020 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-31689472

RESUMEN

Chlorocholine chloride (CCC), a plant growth retardant, may act as an endocrine disruptor. Our previous study showed that pubertal CCC exposure in rats might decrease testosterone (T) synthesis. This study observed the changes in pubertal development and reproduction of male rats exposed to CCC and its underlying mechanisms. Rats were exposed to CCC (0, 75, 137.5 and 200 mg/kg bw/day) from postnatal day 23 to 60. The results showed that CCC treatment delayed the onset of puberty and reduced the relative organ weight of prostate. Seminiferous tubules with deciduous spermatogenic cells were observed in the 200 mg/kg bw/day group. Sexual behavior was inhibited in the 137.5 and 200 mg/kg bw/day groups. Sperm motility, litter size and normalized anogenital distance (AGD) of male pups were decreased in the 137.5 and 200 mg/kg bw/day groups. Serum kisspeptin level and serum and testicular levels of T were reduced in all CCC treated groups. Crucial hormones in hypothalamic-pituitary-testicular (HPT) axis were reduced subsequently after CCC treatment. Collectively, our results demonstrated that CCC might disturb HPT axis through suppressing the secretion of kisspeptin and subsequently lead to delayed puberty onset and impaired reproductive functions.


Asunto(s)
Clormequat/toxicidad , Reproducción/efectos de los fármacos , Maduración Sexual/efectos de los fármacos , Animales , Relación Dosis-Respuesta a Droga , Genitales/anatomía & histología , Genitales/efectos de los fármacos , Genitales/crecimiento & desarrollo , Hormonas Esteroides Gonadales/sangre , Kisspeptinas/metabolismo , Tamaño de la Camada/efectos de los fármacos , Masculino , Tamaño de los Órganos/efectos de los fármacos , Próstata/efectos de los fármacos , Próstata/crecimiento & desarrollo , Ratas , Ratas Sprague-Dawley , Túbulos Seminíferos/efectos de los fármacos , Conducta Sexual Animal/efectos de los fármacos , Motilidad Espermática/efectos de los fármacos , Espermatogénesis/efectos de los fármacos , Testosterona/sangre
6.
Int J Cancer ; 146(3): 759-768, 2020 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-30968961

RESUMEN

Alcohol consumption is associated with higher risk of breast cancer (BC); however, the biological mechanisms underlying this association are not fully elucidated, particularly the extent to which this relationship is mediated by sex hormone levels. Circulating concentrations of estradiol, testosterone, their free fractions and sex-hormone binding globulin (SHBG), were examined in 430 incident BC cases and 645 matched controls among alcohol-consuming postmenopausal women nested within the European Prospective Investigation into Cancer and Nutrition. Mediation analysis was applied to assess whether individual hormone levels mediated the relationship between alcohol intake and BC risk. An alcohol-related hormonal signature, obtained by partial least square (PLS) regression, was evaluated as a potential mediator. Total (TE), natural direct and natural indirect effects (NIE) were estimated. Alcohol intake was positively associated with overall BC risk and specifically with estrogen receptor-positive tumors with respectively TE = 1.17(95%CI: 1.01,1.35) and 1.36(1.08,1.70) for a 1-standard deviation (1-SD) increase of intake. There was no evidence of mediation by sex steroids or SHBG separately except for a weak indirect effect through free estradiol where NIE = 1.03(1.00,1.06). However, an alcohol-related hormonal signature negatively associated with SHBG and positively with estradiol and testosterone was associated with BC risk (odds ratio [OR] = 1.25 [1.07,1.47]) for a 1-SD higher PLS score, and had a statistically significant NIE accounting for a mediated proportion of 24%. There was limited evidence of mediation of the alcohol-BC association by individual sex hormones. However, a hormonal signature, reflecting lower levels of SHBG and higher levels of sex steroids, mediated a substantial proportion of the association.


Asunto(s)
Consumo de Bebidas Alcohólicas/sangre , Neoplasias de la Mama/epidemiología , Posmenopausia/sangre , Anciano , Consumo de Bebidas Alcohólicas/efectos adversos , Consumo de Bebidas Alcohólicas/epidemiología , Neoplasias de la Mama/etiología , Estudios de Casos y Controles , Estradiol/sangre , Femenino , Humanos , Incidencia , Persona de Mediana Edad , Estudios Prospectivos , Globulina de Unión a Hormona Sexual/análisis , Testosterona/sangre
7.
Heart Fail Clin ; 16(1): 11-21, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31735309

RESUMEN

"Chronic heart failure (CHF) is a complex syndrome characterized by symptoms and signs supported by different forms of cardiac impairment. The link between multiple hormonal and metabolic derangements and the development of CHF and the beneficial effects seen with hormonal replacement therapy suggest that a reduction of anabolic pathways might contribute to the onset of CHF. Therefore, an imbalance between anabolic and catabolic forces could be responsible for the development of CHF. There are sufficient evidence to support the screening in patients with CHF of hormonal deficiencies and their correction with replacement therapy."


Asunto(s)
Hormona del Crecimiento/sangre , Insuficiencia Cardíaca/metabolismo , Resistencia a la Insulina , Enfermedades Metabólicas/etiología , Testosterona/sangre , Hormonas Tiroideas/sangre , Biomarcadores/sangre , Insuficiencia Cardíaca/complicaciones , Insuficiencia Cardíaca/tratamiento farmacológico , Terapia de Reemplazo de Hormonas/métodos , Humanos , Enfermedades Metabólicas/tratamiento farmacológico , Enfermedades Metabólicas/metabolismo
8.
Ecotoxicol Environ Saf ; 188: 109875, 2020 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-31706244

RESUMEN

Previous works showed that chronic exposure to Aroclor 1254 disrupted glucose homeostasis and induced insulin resistance in male mice. To further observe the different effects of Aroclor 1254 exposure on the pancreatic α-cells and ß-cells, male mice were exposed to Aroclor 1254 (0, 0.5, 5, 50, 500 µg/kg) for 60 days, the pancreas was performed a histological examination. The results showed that the percentage of apoptosis cell (indicated by TUNEL assay) was increased in both α-cells and ß-cells, as the Aroclor 1254 dose was increased; the proliferation (indicated by PCNA expression) rate of ß-cells was elevated while that of α-cells was not affected, resulting in an increased ß-cell mass and a decreased α-cell mass in a dose-depend manner. The number of Pdx-1 positive ß-cells was significantly increased whereas that of Arx positive α-cells was markedly decreased, indicating an enhanced ß-cell neogenesis and a weakened α-cell neogenesis. The drastically reduction of serum testosterone levels in all the treatments suggested an anti-androgenic potency of Aroclor 1254. The up-regulation of estrogen receptors (ERα and ERß) and androgen receptor in ß-cells might be responsible for the increased ß-cell mass and neogenesis.


Asunto(s)
Antitiroideos/toxicidad , Células Secretoras de Glucagón/efectos de los fármacos , Células Secretoras de Insulina/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Secretoras de Glucagón/metabolismo , Células Secretoras de Glucagón/patología , Proteínas de Homeodominio/metabolismo , Células Secretoras de Insulina/metabolismo , Células Secretoras de Insulina/patología , Masculino , Ratones , Receptores Androgénicos/metabolismo , Receptores Estrogénicos/metabolismo , Testosterona/sangre , Transactivadores/metabolismo , Factores de Transcripción/metabolismo
9.
Life Sci ; 239: 117012, 2019 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-31678279

RESUMEN

BACKGROUND AND PURPOSE: Reduced male fertility has been regarded as a serious complication of rheumatoid arthritis. Phytochemicals have been described as protective agents against rheumatoid arthritis-linked testicular impairment. The current study aimed to investigate the potential protective effects of ellagic acid on rheumatoid arthritis-evoked testicular dysfunction vis-à-vis the reference anti-inflammatory celecoxib. EXPERIMENTAL APPROACH: Ellagic acid (50 mg/kg/day) and celecoxib (5 mg/kg/day) were administered orally for 20 days in adjuvant-induced arthritic rats. KEY FINDINGS: Current data revealed that ellagic acid counteracted rheumatoid arthritis-evoked testicular histopathologic changes, disrupted sperm characteristics and low gonadosomatic index with comparable efficacy to celecoxib. Ellagic acid also enhanced the testicular steroidogenesis via upregulating the gene expression of 3ß-hydroxysteroid dehydrogenase, 17ß-hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein with consequent boosting of serum testosterone. Notably, ellagic acid attenuated the testicular inflammatory responses through suppression of myeloperoxidase, tumor necrosis factor-α and cyclo-oxygenase-2 protein expression together with enhancing the anti-inflammatory signal interleukin 10. Ellagic acid also curbed the redox alterations via lowering the production of lipid peroxides and nitric oxide and elevation of the anti-oxidant reduced glutathione. In support of cell survival, ellagic acid combated testicular apoptosis through downregulating caspase-3 protein expression. SIGNIFICANCE: The present work accentuates the beneficial actions of ellagic acid in rheumatoid arthritis-incurred testicular impairment and disrupted spermatogenesis via combating the inflammatory, oxidative and apoptotic aberrations.


Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Antiinflamatorios no Esteroideos/uso terapéutico , Apoptosis/efectos de los fármacos , Artritis Reumatoide/complicaciones , Ácido Elágico/farmacología , Ácido Elágico/uso terapéutico , Enfermedades Testiculares/tratamiento farmacológico , Enfermedades Testiculares/etiología , Animales , Caspasa 3/efectos de los fármacos , Celecoxib/farmacología , Celecoxib/uso terapéutico , Regulación hacia Abajo/efectos de los fármacos , Masculino , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Espermatozoides/efectos de los fármacos , Esteroides/biosíntesis , Testosterona/sangre
10.
Int Braz J Urol ; 45(5): 1043-1054, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31626524

RESUMEN

OBJECTIVE: Anacyclus Pyrethrum (AP) and Tribulus Terrestris (TT) have been reported as male infertility treatment in several studies; however, in Iranian traditional medicine these two plants are prescribed simultaneously. In this study, we aimed to determine the effects of AP and TT extracts both separately and simultaneously on the male Wistar rat fertility parameters. MATERIALS AND METHODS: 32 male Wistar rats were divided into 4 groups: Control, TT, AP, and AT treated groups. Treatment continued for 25 days and rats were weighed daily. Their testes were dissected for histological studies. Sperm analysis including sperm count, viability and motility were performed. Serum was obtained to evaluate testosterone, LH and FSH levels. Histological studies were conducted to study Leydig, and Sertoli cells, spermatogonia and spermatid cell numbers, and to measure seminiferous diameter and epithelium thickness. RESULTS: Sperm count increased in all the treatment groups. Sperm viability and motility in AT and AP groups were elevated. TT and AT groups showed signifi cantly increased testosterone level compared to control group (P=004, P=0.000, respectively) and TT, AP and AT treatment groups showed increased LH level (P=0.002, P=0.03 and P=0.000, respectively) compared to control, while only AT group showed increased FSH (p=0.006) compared to control. Histological studies showed signifi cant increase of spermatogonia, Leydig and Sertoli cell numbers and epithelial thickness in AT group compared to other groups. All the treatment groups had higher number of Leydig, spermatogonia and spermatid cells. CONCLUSION: TT and AP improved sexual parameters; however, their simultaneous administration had higher improving effects on studied parameters.


Asunto(s)
Chrysanthemum cinerariifolium/química , Infertilidad Masculina/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Tribulus/química , Animales , Peso Corporal , Fertilidad/efectos de los fármacos , Hormona Folículo Estimulante/sangre , Humanos , Hormona Luteinizante/sangre , Masculino , Tamaño de los Órganos , Extractos Vegetales/farmacología , Ratas Wistar , Valores de Referencia , Reproducibilidad de los Resultados , Recuento de Espermatozoides , Motilidad Espermática/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Testosterona/sangre , Resultado del Tratamiento
11.
Anal Bioanal Chem ; 411(28): 7519-7528, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31630222

RESUMEN

Testosterone in human serum is commonly tested in clinical laboratories using immunoassay methods as well as liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. To standardize and ensure the accuracy of the measurement results, reference procedures with higher metrological order are required. A simple measurement procedure based on one-step liquid-liquid extraction (LLE) and liquid chromatography-isotope dilution tandem mass spectrometry (LC-IDMS/MS) was developed for total testosterone in human serum. The procedure involved serum spiked with 13C3-testosterone, equilibration for 2 h, and extraction with an organic solvent. Testosterone certified reference material (CRM) was used as the calibration standard to ensure the traceability to the International System of Units (SI). Testosterone in serum CRMs from the National Institute for Standards and Technology (NIST) and LGC were used to validate the accuracy of the newly developed method. The deviations of the obtained values from the NIST and LGC certified values ranged from -0.55% to 0.45%. Similarly, the coefficient of variations (CVs) of the replicate measurements were in the range of 0.55% and 0.78%, respectively. The relative expanded uncertainties were comparable with those of the certified materials. The newly developed LC-IDMS/MS procedure demonstrated adequate trueness and precision, and was simple to perform. The method can be used for value assignment of testosterone in external quality assessment (EQA) materials as well as certification of CRMs in the future. Graphical abstract.


Asunto(s)
Extracción Líquido-Líquido/métodos , Espectrometría de Masas en Tándem/métodos , Testosterona/sangre , Calibración , Cromatografía Liquida/métodos , Humanos , Técnicas de Dilución del Indicador , Isótopos , Estándares de Referencia , Reproducibilidad de los Resultados , Testosterona/normas , Incertidumbre
12.
BMC Complement Altern Med ; 19(1): 270, 2019 Oct 17.
Artículo en Inglés | MEDLINE | ID: mdl-31623582

RESUMEN

BACKGROUND: Benign prostatic hyperplasia (BPH) is a pathological condition affecting older men. BPH complications often lead to deterioration in the quality of life. Serenoa repens (Saw Palmetto) is used for treating lower urinary tract infections in traditional medicine. METHODS: This study was performed to compare the efficacy of ß-sitosterol enriched saw palmetto oil (VISPO) and conventional saw palmetto oil (SPO) extracted using supercritical fluid extraction, in alleviating the BPH complications using testosterone-induced BPH model rats. The animals received testosterone (5 mg/kg s.c.) with or without SPO and VISPO (200 and 400 mg/kg b.w.) or Finasteride (1 mg/kg b.w.) p.o. for 28 days. At the end of the experiment, overnight fasted animals were euthanized, blood samples collected for serum analysis of testosterone. Prostate tissue histomorphology was examined by hematoxylin and eosin (H&E) staining. Western blot analysis was performed using prostate tissue homogenates. RESULTS: VISPO exhibited superior efficacy compared to SPO as evident from the significant decrease in prostate weight to body weight ratio, serum testosterone level and increase in growth inhibition of prostate tissue compared to BPH group (p < 0.001). Histological examination of prostate tissue samples showed that VISPO treatment was comparatively better than SPO in improving the hyperplastic patterns. Further, VISPO significantly regulated the expression of inflammatory and apoptotic marker proteins in BPH rats. CONCLUSION: Our data provide experimental evidence that ß-sitosterol enriched saw palmetto oil could be higher efficacious in treating the BPH complications compared to the conventional saw palmetto oil preparations.


Asunto(s)
Fitosteroles/administración & dosificación , Extractos Vegetales/administración & dosificación , Hiperplasia Prostática/tratamiento farmacológico , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteína X Asociada a bcl-2/genética , Animales , Cromatografía con Fluido Supercrítico , Humanos , Masculino , Fitosteroles/aislamiento & purificación , Fitoterapia , Extractos Vegetales/aislamiento & purificación , Próstata/efectos de los fármacos , Próstata/inmunología , Hiperplasia Prostática/sangre , Hiperplasia Prostática/inducido químicamente , Hiperplasia Prostática/inmunología , Proteínas Proto-Oncogénicas c-bcl-2/inmunología , Ratas , Ratas Wistar , Serenoa/química , Sitoesteroles/administración & dosificación , Sitoesteroles/aislamiento & purificación , Testosterona/efectos adversos , Testosterona/sangre , Proteína X Asociada a bcl-2/inmunología
13.
Environ Pollut ; 255(Pt 2): 113317, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31610502

RESUMEN

Aflatoxin B1 (AFB1) is a hazard environmental pollutants and the most toxic one of all the aflatoxins. AFB1 can cause a serious impairment to testicular development and spermatogenesis, yet the underlying mechanisms remain inconclusive. Oxidative stress acts as a master mechanism of AFB1 toxicity, and can promote autophagy. Abnormal autophagy resulted in testicular damage and spermatogenesis disorders. The objective of this study was to explore the effect of AFB1 on autophagy in mice testis and its potential mechanisms. In this study, male mice were intragastrically administered with 0, 0.375, 0.75 or 1.5 mg/kg body weight AFB1 for 30 days. We found that AFB1 induced testicular damage, reduced serum testosterone level and impaired sperm quality accompanied with the elevation of oxidative stress and germ cell apoptosis. Interestingly, we observed increasing numbers of autophagosomes in AFB1-exposed mice testis. Meanwhile, AFB1 caused testis abnormal autophagy with the characterization of increased expressions of LC3, Beclin-1, Atg5 and p62. Furthermore, AFB1 downregulated the expressions of PI3K, p-AKT and p-mTOR in mice testis. Taken together, our data indicated AFB1 induced testicular damage and promoted autophagy, which were associated with oxidative stress-related PI3K/AKT/mTOR signaling pathway in mice testis.


Asunto(s)
Aflatoxina B1/toxicidad , Autofagia/efectos de los fármacos , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Serina-Treonina Quinasas TOR/metabolismo , Testículo/patología , Animales , Apoptosis/efectos de los fármacos , Autofagosomas/efectos de los fármacos , Beclina-1/metabolismo , Masculino , Ratones , Estrés Oxidativo/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Espermatogénesis/fisiología , Espermatozoides/metabolismo , Testículo/embriología , Testosterona/sangre
14.
Environ Pollut ; 255(Pt 2): 113316, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31610511

RESUMEN

Paraquat is a fast and non-selective herbicide that is widely used in crop cultivation and conservation tillage systems. Animal experiments have shown that paraquat decreases sperm quality and testicular organ coefficient, but its effects on the development of Leydig cells remain unclear. The objective of the current study was to investigate the effects of paraquat exposure on the Leydig cell development in rats during puberty. Twenty-eight male 35-day-old Sprague-Dawley rats were divided into 4 groups: 0, 0.5, 2.0, and 8 mg kg-1 d-1 paraquat. Paraquat was gavaged for 10 d. Adult Leydig cells were isolated and treated with paraquat for 24 h. Paraquat in vivo significantly decreased body and testis weights at 8 mg kg-1 and lowered serum testosterone levels at 2 and 8 mg kg-1 without affecting the levels of serum luteinizing hormone and follicle-stimulating hormone. Paraquat did not alter Leydig cell number and PCNA labeling index. Real-time PCR showed that paraquat down-regulated the expression of Lhcgr, Scarb1, Cyp11a1, Cyp17a1, and Hsd17b3 genes and their proteins at 2 or 8 mg kg-1, while it up-regulated the expression of Srd5a1 at 8 mg kg-1. Paraquat increased ROS and decreased testosterone production by Leydig cells at 1 and 10 µM after in vitro 24-h exposure. Vitamin E (40 µg/ml) reversed paraquat-induced ROS and suppression of testosterone synthesis in vitro. In conclusion, paraquat directly delays Leydig cell differentiation to block testosterone synthesis via down-regulating the expression of critical testosterone synthesis-related genes and up-regulating the expression of testosterone metabolic enzyme (Srd5a1) gene and possibly via increasing ROS production.


Asunto(s)
Herbicidas/toxicidad , Células Intersticiales del Testículo/efectos de los fármacos , Paraquat/toxicidad , Animales , Diferenciación Celular/efectos de los fármacos , Regulación hacia Abajo , Hormona Folículo Estimulante/sangre , Herbicidas/metabolismo , Hormona Luteinizante/sangre , Masculino , Ratas , Ratas Sprague-Dawley , Maduración Sexual , Esteroide 17-alfa-Hidroxilasa/metabolismo , Testículo/efectos de los fármacos , Testosterona/sangre , Regulación hacia Arriba
15.
J Sports Med Phys Fitness ; 59(9): 1435-1441, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31610637

RESUMEN

BACKGROUND: This study investigated the effects of coffee ingestion with supplemental caffeine (CAF) on serum testosterone (T) responses to exercise in recreationally strength-trained males. METHODS: Subjects ingested 6 mg/kg body weight of caffeine via 12 ounces of coffee (CAF) supplemented with anhydrous caffeine or decaffeinated (DEC) coffee prior to exercise in a randomized, within-subject, crossover design. The exercise session consisted of 21 minutes of high-intensity interval cycling (alternating intensities at power outputs associated with 2.0 mmol/L lactate for two minutes and 4.0 mmol/L lactate for one minute) followed by resistance exercise (seven exercises, three sets of ten repetitions, 65% 1RM, one-minute rest periods). Subjects also completed repetitions to fatigue tests and soreness scales to determine muscle recovery 24 hours following the exercise. RESULTS: T was elevated immediately and 30-minutes post-exercise by 20.5% and 14.3% respectively (P<0.05). There was no main effect for treatment and no exercise x treatment interaction. There were no differences in repetitions to fatigue or soreness between treatments (P>0.05). No relationships were observed between T and any proxy of recovery. CONCLUSIONS: While past literature suggests caffeine may enhance T post-exercise, data from the current study suggest that augmented T response is not evident following anhydrous caffeine added to coffee. The duration of T elevation indicates that this protocol is beneficial to creating long-lasting increases in serum testosterone.


Asunto(s)
Cafeína/metabolismo , Entrenamiento de Intervalos de Alta Intensidad/métodos , Entrenamiento de Resistencia/métodos , Testosterona/sangre , Adulto , Cafeína/administración & dosificación , Café , Estudios Cruzados , Humanos , Ácido Láctico/sangre , Masculino , Adulto Joven
16.
Medicine (Baltimore) ; 98(39): e16788, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31574794

RESUMEN

BACKGROUND: The aim of this study was to observe the effect and safety of Heyan Kuntai Capsule (HYKT) on glucose and lipid metabolism in patients with polycystic ovary syndrome (PCOS). METHODS: Hundred patients with PCOS were randomly divided into HYKT group (n = 50) and placebo groups (n = 50) in which the individuals were treated with HYKT and its placebo continuously for 6 months. Meanwhile, all participants received health education (such as exercise and diet). The primary outcomes were serum sex hormone levels, a series of blood lipid, fasting and postprandial 2 hours blood glucose. Body mass index (BMI), waist-hip ratio (WHR), insulin, homeostatic model assessment of insulin resistance (HOMA-IR), and insulin-sensitive index (ISI) were also observed. In addition, adverse events were recorded to evaluate the drug safety. RESULTS: After treatment, the BMI and WHR of all the patients were decreased. The fasting and postprandial 2 hours blood glucose levels were significantly declined when treated with HYKT, which were not observed in the placebo group. Similarly, serum sex hormones including luteinizing hormone (LH), LH/follicle-stimulating hormone (FSH), and testosterone were lowered after treated with HYKT instead of the placebo. Besides, blood lipids outcomes such as total cholesterol, triglyceride, and low-density lipoprotein cholesterol, as well as insulin and HOMA-IR were decreased with significance in HYKT group when compared with those in the placebo group, whereas high-density lipoprotein cholesterol and ISI increased obviously. CONCLUSION: HYKT showed the effect on ameliorating the glucose and lipid metabolism disorder and improving insulin resistance and increase insulin sensitivity of PCOS patients, which is similar to insulin sensitizing agent.


Asunto(s)
Glucemia/metabolismo , Medicamentos Herbarios Chinos/efectos adversos , Medicamentos Herbarios Chinos/uso terapéutico , Lípidos/sangre , Síndrome del Ovario Poliquístico/sangre , Síndrome del Ovario Poliquístico/tratamiento farmacológico , Adolescente , Adulto , Índice de Masa Corporal , Método Doble Ciego , Estradiol/sangre , Femenino , Hormona Folículo Estimulante/sangre , Humanos , Insulina/sangre , Resistencia a la Insulina , Hormona Luteinizante/sangre , Prolactina/sangre , Testosterona/sangre , Relación Cintura-Cadera , Adulto Joven
17.
Environ Pollut ; 255(Pt 1): 113097, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31520908

RESUMEN

Decabromodiphenyl ether (BDE-209) is commonly used as a flame retardant, usually in products that were utilized in electronic equipment, plastics, furniture and textiles. To identify the impacts of BDE-209 on the male reproductive system and the underlying toxicological mechanisms, 40 male ICR mice were randomly divided into four groups, which were then exposed to BDE-209 at 0, 7.5, 25 and 75 mg kg-1 d-1 for four weeks, respectively. With regard to the in vitro study, GC-2spd cells were treated with BDE-209 at 0, 2, 8 and 32 µg mL-1 for 24 h, respectively. The results from the in vivo experiments showed that BDE-209 resulted in damage to the testis structure, led to cell apoptosis in testis and decreased sperm number and motility, while sperm malformation rates were significantly increased. Moreover, BDE-209 could induce oxidative stress with decreased testosterone levels, result in DNA damage and activate DNA damage response signaling pathways (ATM/Chk2, ATR/Chk1 and DNA-PKcs/XRCC4/DNA ligase Ⅳ). The data from the in vitro experiments showed that BDE-209 led to cytotoxicity by reducing cell viability and increasing LDH release as well. BDE-209 also induced DNA strand breaks, cell cycle arrest at G1 phase and elevated reactive oxygen species (ROS) level in GC-2 cells. These results suggested that BDE-209 could lead to male reproductive toxicity by inducing DNA damage and failure of DNA damage repair which resulted in cell cycle arrest and apoptosis of spermatogenic cell. The present study provided new evidence to elucidate the potential mechanism of male reproductive toxicity induced by BDE-209.


Asunto(s)
Apoptosis/efectos de los fármacos , Daño del ADN/efectos de los fármacos , Retardadores de Llama/toxicidad , Puntos de Control de la Fase G1 del Ciclo Celular/efectos de los fármacos , Éteres Difenilos Halogenados/toxicidad , Espermatozoides/patología , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Reparación del ADN/genética , Masculino , Ratones , Ratones Endogámicos ICR , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de los fármacos , Recuento de Espermatozoides , Motilidad Espermática/efectos de los fármacos , Espermatozoides/citología , Testículo/citología , Testículo/patología , Testosterona/sangre
18.
Am J Vet Res ; 80(10): 931-942, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31556711

RESUMEN

OBJECTIVE: To characterize physical examination, plasma biochemical, and ultrasonographic findings in aquarium-housed, managed semiwild, and wild southern stingrays (Hypanus americanus) with and without reproductive disease. ANIMALS: Southern stingrays from aquarium (n = 48), lagoon (managed semiwild; 34), and wild (12) habitats. PROCEDURES: Limited, opportunistic prosections were performed of presumed anatomically normal wild southern stingrays and compared with findings for aquarium-housed stingrays with reproductive disease. Ultrasonographic video data from both groups were used to assign a score (1 to 5) indicating increasing severity of ovarian and uterine reproductive disease. Plasma total 17ß-estradiol, estrone, progesterone, and testosterone concentrations were measured with enzyme immunoassays validated for use in southern stingrays. RESULTS: Ultrasonographic ovarian scores were significantly correlated with uterine scores. No reproductive disease was detected in semiwild or wild stingrays, but 65% (31/48) of aquarium-housed stingrays had developing or advanced reproductive disease (ie, ultrasonographic ovarian or uterine score of 4 or 5). Significant correlations were identified between ovarian and uterine disease status and plasma concentrations of all steroid hormones except testosterone. CONCLUSIONS AND CLINICAL RELEVANCE: Findings suggested that ultrasonography and plasma hormone concentrations may be useful in the identification of reproductive disease and determination of disease severity in southern stingrays.


Asunto(s)
Enfermedades de los Peces/diagnóstico por imagen , Enfermedades del Ovario/veterinaria , Enfermedades Uterinas/veterinaria , Animales , Animales Salvajes , Estradiol/sangre , Femenino , Enfermedades de los Peces/sangre , Explotaciones Pesqueras , Enfermedades del Ovario/sangre , Enfermedades del Ovario/diagnóstico por imagen , Progesterona/sangre , Reproducción , Salud Reproductiva , Testosterona/sangre , Ultrasonografía/veterinaria , Enfermedades Uterinas/sangre , Enfermedades Uterinas/diagnóstico por imagen
19.
Harefuah ; 158(9): 568-570, 2019 Sep.
Artículo en Hebreo | MEDLINE | ID: mdl-31507105

RESUMEN

AIMS: To examine statistical correlation between mSASSS and serum levels of testosterone in males suffering from AS. BACKGROUND: Ankylosing spondylitis (AS) is a chronic progressive inflammatory rheumatic disease primarily involving sacroiliac joints and spine. Structural damage, caused by AS, manifests with development of vertebral syndesmophytes and can be calculated as units of modified Spinal Ankylosing Spondylitis Syndesmophyte Score (mSASSS). The rate of growth of spinal syndesmophytes differs among individual AS patients, while male patients develop significantly more structural damage compared to females in general. METHODS: Twenty males with AS known for at least 5 years (average disease duration 12.8 years) and aged between 25 to 40 years donated 5 ml of peripheral blood for serum testosterone assay, and underwent X-ray films of cervical and lumbar spine. The mSASSS was calculated and correlation with serum testosterone levels was examined using Pearson correlation test. RESULTS: The mSASSS values of patients included in the final analysis ranged from 0-14 units and testosterone levels ranged from 8.4-25.5 nmol/L. No significant correlation was found between mSASSS values and testosterone levels in this cohort. CONCLUSIONS: This study did not find statistical correlation between mSASSS and serum levels of testosterone in males suffering from AS.


Asunto(s)
Espondilitis Anquilosante/sangre , Testosterona/sangre , Adulto , Progresión de la Enfermedad , Femenino , Humanos , Vértebras Lumbares , Masculino , Radiografía , Índice de Severidad de la Enfermedad , Columna Vertebral
20.
Chemosphere ; 234: 909-916, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31519099

RESUMEN

T-2 toxin could impair male reproductive function. But, the toxicity mechanism is still unclear. In this study, male Kunming mice were orally administrated with T-2 toxin at the doses of 0, 0.5, 1 or 2 mg/kg body weight for 28 days. The fertility, body weight, reproductive organs volume, daily sperm production (DSP), and sperm malformation rate were detected. The expressions of testosterone (T) biosynthetic enzymes, luteinizing hormone (LH)-receptor, follicle stimulating hormone (FSH)-receptor and androgen binding protein (ABP) in testis were detected. The serum hormone level of gonadotropin-releasing hormone (GnRH), FSH, LH, T and progesterone (P), and the mRNA expression of GnRH, GnRH-receptor, LH and FSH were measured. These results demonstrated that T-2 toxin decreased body weight, reproductive organs volume and DSP, increased sperm malformation rate. T-2 toxin impaired fertility by decreasing the mating index, fertility index, numbers of implantation sites and viable fetuses, and increasing the number of animal with resorptions. Meantime, T-2 suppressed testicular function by inhibiting T biosynthesis and decreasing FSHR, LHR and ABP expression. Furthermore, the serum reproductive hormone contents and key factors expression of hypothalamic-pituitary-testis (HPT) axis were decreased by T-2 toxin. In summary, T-2 toxin impaired the male fertility by disrupting HPT axis and impairing testicular function.


Asunto(s)
Toxina T-2/toxicidad , Testículo/efectos de los fármacos , Animales , Fertilidad , Hormona Folículo Estimulante/sangre , Hormona Liberadora de Gonadotropina/metabolismo , Hormona Luteinizante/sangre , Masculino , Ratones , Receptores de HFE/metabolismo , Receptores LHRH/metabolismo , Reproducción , Espermatozoides/metabolismo , Testículo/metabolismo , Testosterona/sangre
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