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1.
Biochimie ; 158: 34-42, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30557594

RESUMEN

Inflammation of temporomandibular joint (TMJ) tissues are the most common cause of pain conditions associated with temporomandibular disorders (TMDs). After a tissue and/or neural damage, the inflammatory response is characterized by plasma extravasation and leukocytes infiltration in the TMJ tissues, which in turn, release inflammatory cytokines cascades responsible for inflammatory pain. Lectins are glycoproteins widely distributed in nature that may exhibit anti-inflammatory properties. This study demonstrated by molecular docking and MM/PBSA that the lectin from Dioclea violacea (DVL) interacts favorably with α-methyl-D-mannoside, N-acetyl-D-glucosamine, and core1-sialyl-Lewis X which are associated with leukocytes migration during an inflammatory response. Wistar rats pretreated with intravenously injection of DVL demonstrated a significant inhibition of plasma extravasation induced by carrageenan (a non-neurogenic inflammatory inductor) and mustard oil (a neurogenic inflammatory inductor) in the TMJ periarticular tissues (p < 0.05; ANOVA, Tukey's test). In addition, DVL significantly reduced carrageenan-induced leukocyte migration in the TMJ periarticular tissues mediated by down-regulation of ICAM-1 expression. These results suggest a potential anti-inflammatory effect of DVL in inflammatory conditions of TMJ.


Asunto(s)
Antiinflamatorios , Dioclea/química , Molécula 1 de Adhesión Intercelular/biosíntesis , Leucocitos/metabolismo , Lectinas de Plantas , Trastornos de la Articulación Temporomandibular/tratamiento farmacológico , Articulación Temporomandibular/metabolismo , Animales , Antiinflamatorios/química , Antiinflamatorios/farmacología , Movimiento Celular/efectos de los fármacos , Regulación de la Expresión Génica/efectos de los fármacos , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Inflamación/patología , Leucocitos/patología , Masculino , Simulación del Acoplamiento Molecular , Lectinas de Plantas/química , Lectinas de Plantas/farmacología , Ratas , Ratas Wistar , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/metabolismo , Trastornos de la Articulación Temporomandibular/patología
2.
Inflamm Res ; 67(5): 407-422, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29362850

RESUMEN

OBJECTIVE AND DESIGN: To investigate the role of heme oxygenase-1 (HO-1), carbon monoxide (CO), and biliverdin (BVD) in the zymosan-induced TMJ arthritis in rats. MATERIALS AND METHODS: Mechanical threshold was assessed before and 4 h after TMJ arthritis induction in rats. Cell influx, myeloperoxidase activity, and histological changes were measured in the TMJ lavages and tissues. Trigeminal ganglion and periarticular tissues were used for HO-1, TNF-α, and IL-1ß mRNA time course expression and immunohistochemical analyses. Hemin (0.1, 0.3, or 1 mg kg-1), DMDC (0.025, 0.25, or 2.5 µmol kg-1), biliverdin (1, 3, or 10 mg kg-1), or ZnPP-IX (1, 3 or 9 mg kg-1) were injected (s.c.) 60 min before zymosan. ODQ (12.5 µmol kg-1; s.c.) or glibenclamide (10 mg kg-1; i.p.) was administered 1 h and 30 min prior to DMDC (2.5 µmol kg-1; s.c), respectively. RESULTS: Hemin (1 mg kg-1), DMDC (2.5 µmol kg-1), and BVD (10 mg kg-1) reduced hypernociception and leukocyte migration, which ZnPP (3 mg kg-1) enhanced. The effects of DMDC were counteracted by ODQ and glibenclamide. The HO-1, TNF-α, and IL-1ß mRNA expression and immunolabelling increased. CONCLUSIONS: HO-1/BVD/CO pathway activation provides anti-nociceptive and anti-inflammatory effects on the zymosan-induced TMJ hypernociception in rats.


Asunto(s)
Biliverdina/fisiología , Monóxido de Carbono/fisiología , GMP Cíclico , Hemo-Oxigenasa 1/fisiología , Canales KATP , Nocicepción/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Animales , Artritis/inducido químicamente , Biliverdina/genética , Citocinas/metabolismo , Regulación hacia Abajo/efectos de los fármacos , Hemo-Oxigenasa 1/genética , Masculino , Umbral del Dolor , Peroxidasa/metabolismo , Bloqueadores de los Canales de Potasio/farmacología , Ratas , Ratas Wistar , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/patología , Ganglio del Trigémino/efectos de los fármacos , Zimosan
3.
Oral Dis ; 24(4): 600-610, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29069539

RESUMEN

OBJECTIVE: This study evaluated low-intensity pulsed ultrasound effects for temporomandibular joint osteoarthritis in adult rats. MATERIAL AND METHODS: Osteoarthritis-like lesions were induced in 24 adult rats' temporomandibular joints with low-dose mono-iodoacetate injections. The rats were divided into four groups: control and mono-iodoacetate groups, injected with contrast media and mono-iodoacetate, respectively, at 12 weeks and observed until 20 weeks; and low-intensity pulsed ultrasound and mono-iodoacetate + low-intensity pulsed ultrasound groups, injected with contrast media and mono-iodoacetate, respectively, at 12 weeks with low-intensity pulsed ultrasound performed from 16 to 20 weeks. Condylar bone mineral density, bone mineral content and bone volume were evaluated weekly with microcomputed tomography. Histological and immunohistochemical staining for matrix metalloproteinases-13 was performed at 20 weeks. RESULTS: At 20 weeks, the mono-iodoacetate + low-intensity pulsed ultrasound group showed significantly higher bone mineral density, bone mineral content and bone volume than the mono-iodoacetate group; however, these values remained lower than those in the other two groups. On histological and immunohistochemical analysis, the chondrocytes were increased, and fewer matrix metalloproteinases-13 immunopositive cells were identified in the mono-iodoacetate + low-intensity pulsed ultrasound group than mono-iodoacetate group. CONCLUSIONS: Low-intensity pulsed ultrasound for 2 weeks may have therapeutic potential for treating temporomandibular joint osteoarthritis lesions.


Asunto(s)
Osteoartritis/terapia , Trastornos de la Articulación Temporomandibular/terapia , Terapia por Ultrasonido , Animales , Densidad Ósea , Condrocitos , Ácido Yodoacético , Masculino , Cóndilo Mandibular/diagnóstico por imagen , Cóndilo Mandibular/metabolismo , Cóndilo Mandibular/patología , Metaloproteinasa 13 de la Matriz/metabolismo , Osteoartritis/inducido químicamente , Osteoartritis/diagnóstico por imagen , Osteoartritis/patología , Ratas , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Trastornos de la Articulación Temporomandibular/patología , Microtomografía por Rayos X
4.
J Oral Facial Pain Headache ; 32(1): 75­83, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29145524

RESUMEN

AIMS: To determine the involvement of tumor necrosis factor alpha (TNFα) signaling in the trigeminal ganglion (TG) in the mechanical hypersensitivity of the masseter muscle during temporomandibular joint (TMJ) inflammation. METHODS: A total of 55 male Sprague-Dawley rats were used. Following injection of Complete Freund's Adjuvant into the TMJ, the mechanical sensitivities of the masseter muscle and the overlying facial skin were measured. Satellite glial cell (SGC) activation and TNFα expression in the TG were investigated immunohistochemically, and the effects of their inhibition on the mechanical hypersensitivity of the masseter muscle were also examined. Student t test or two-way repeated-measures analysis of variance followed by Bonferroni multiple comparisons test were used for statistical analyses. P < .05 was considered to reflect statistical significance. RESULTS: Mechanical allodynia in the masseter muscle was induced without any inflammatory cell infiltration in the muscle after TMJ inflammation. SGC activation and an increased number of TNFα-immunoreactive cells were induced in the TG following TMJ inflammation. Intra-TG administration of an inhibitor of SGC activity or of TNFα-neutralizing antibody depressed both the increased number of TG cells encircled by activated SGCs and the mechanical hypersensitivity of the masseter following TMJ inflammation. CONCLUSION: These findings suggest that persistent masseter hypersensitivity associated with TMJ inflammation was mediated by SGC-TG neuron interactions via TNFα signaling in the TG.


Asunto(s)
Músculo Masetero/metabolismo , Trastornos de la Articulación Temporomandibular/metabolismo , Ganglio del Trigémino/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Animales , Anticuerpos Neutralizantes , Modelos Animales de Enfermedad , Adyuvante de Freund , Inflamación/inducido químicamente , Masculino , Mecanotransducción Celular , Dolor/etiología , Estimulación Física , Ratas , Ratas Sprague-Dawley , Articulación Temporomandibular/metabolismo , Trastornos de la Articulación Temporomandibular/inducido químicamente , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores
5.
J Investig Clin Dent ; 9(1)2018 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-28834423

RESUMEN

Khat or qat (Catha edulis) is a plant that grows in East Africa and southern Arabia. The leaves and twigs of this small tree are chewed by several millions of people worldwide for their stimulating amphetamine-like effects. The reported prevalence of khat chewing in Europe and the USA is on the rise, especially with global migration. Long-term khat chewing has several detrimental general and oral health effects. The aim of the present study was to review the current literature regarding khat use and its association with oral and dental diseases, with particular emphasis on its link with oral keratotic white lesions and oral cancer. We searched the literature to identify all relevant articles. Studies showed that khat is associated with several oral and dental conditions, including keratotic white lesions, mucosal pigmentation, periodontal disease, tooth loss, plasma cell stomatitis, and xerostomia. There are limited data on the incidence of dental caries among khat chewers. The evidence that khat chewing is a risk factor for oral cancer is still weak, and is mainly based on anecdotal case reports and uncontrolled studies.


Asunto(s)
Catha/efectos adversos , Salud Bucal , Extractos Vegetales/efectos adversos , Anfetamina/efectos adversos , Caries Dental/inducido químicamente , Gingivitis/inducido químicamente , Humanos , Masticación , Microbiota/efectos de los fármacos , Mucosa Bucal/efectos de los fármacos , Neoplasias de la Boca/inducido químicamente , Enfermedades Periodontales/inducido químicamente , Periodoncio/efectos de los fármacos , Pigmentación/efectos de los fármacos , Hojas de la Planta/química , Factores de Riesgo , Glándulas Salivales/efectos de los fármacos , Estomatitis/inducido químicamente , Trastornos de la Articulación Temporomandibular/inducido químicamente , Uso de Tabaco/efectos adversos , Decoloración de Dientes/inducido químicamente , Pérdida de Diente/inducido químicamente , Xerostomía/inducido químicamente
6.
J Pharmacol Toxicol Methods ; 88(Pt 1): 100-108, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28797764

RESUMEN

Temporomandibular joint (TMJ) disorders are a group of conditions that result in TMJ pain, which frequently limits basic daily activities. Experimental models that allow the study of the mechanisms underlying these inflammatory and pain conditions are of great clinical relevance. The aim of this study was to evaluate nociception, inflammation and participation of the macrophage/microglia cells in the arthritis of the TMJ induced by two phlogistic agents. 84 rats were divided into 2 groups: Zy, which received zymosan intra-articularly, or Cg, which received carrageenan intra-articularly. Mechanical nociception, total leukocyte influx to the synovial fluid and histopathological analyses were evaluated in the TMJ. The participation of macrophage/microglia located in trigeminal ganglia (TG) and in the subnucleus caudalis (V-SnC) was assessed immunohistochemically. Both agents induced mechanical hyperalgesia 6h after the induction, but a more persistent algesic state was perceived in the Cg group, which lasted for 120h. Even though both groups presented increased leukocyte influx, the Zy-group presented a more intense influx. Zymosan recruited resident macrophage in the trigeminal ganglia 24h after the injection. In the V-SnC, the group Cg presented a more prolonged immunolabeling pattern in comparison with the group Zy. It can be concluded that zymosan induced a more intense infiltrate and peripheral nervous changes, while Cg lead to a moderate TMJ inflammation with prominent changes in the V-SnC.


Asunto(s)
Artritis/fisiopatología , Hiperalgesia/fisiopatología , Dimensión del Dolor/métodos , Dolor/fisiopatología , Trastornos de la Articulación Temporomandibular/fisiopatología , Animales , Artritis/inducido químicamente , Artritis/diagnóstico , Artritis/patología , Carragenina/farmacología , Modelos Animales de Enfermedad , Hiperalgesia/inducido químicamente , Hiperalgesia/patología , Inyecciones Intraarticulares , Macrófagos/efectos de los fármacos , Macrófagos/patología , Masculino , Microglía/efectos de los fármacos , Microglía/patología , Nocicepción/efectos de los fármacos , Nocicepción/fisiología , Dolor/inducido químicamente , Dolor/patología , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/patología , Ganglio del Trigémino/citología , Ganglio del Trigémino/efectos de los fármacos , Zimosan/farmacología
7.
Rev Stomatol Chir Maxillofac Chir Orale ; 117(4): 298-301, 2016 Sep.
Artículo en Francés | MEDLINE | ID: mdl-27554490

RESUMEN

INTRODUCTION: Temporomandibular disorders (TMDs) affect the masticatory muscles and the temporomandibular joints (TMJs). TMDs most often result from occlusal and/or muscular disorders and are then called primary or idiopathic TMDs. Less frequently, TMDs are related to local (trauma, infection) or general (rheumatoid arthritis) causes and are then called secondary TMDs. A little known iatrogenic cause of secondary TDM is the osteoarthritis that may be induced by intra-articular cortisone injections. We report one case of condylar lysis that occurred after one single intra-articular cortisone injection. OBSERVATION: A 62-years-old woman consulted for a long-lasting TMD on the left side manifesting itself through pain and noise. She benefited one year before from an intra-articular injection of cortisone by her rheumatologist for repeated closed lock of her left TMJ. Physical examination showed limited mouth opening with deviation on the left side. Lateral movements on the right side were impossible. The panoramic X-ray showed a condylar lysis on the left side that was on the CT scan. MRI additionally showed an anteriorly displaced and severely reshaped disc and an articular inflammation without intra-articular effusion. DISCUSSION: TMJ osteoarthritis secondary to unique or repeated intra-articular steroid injections are little-known. They are clinically expressed as typical TMDs and characterized on X-rays by condylar lysis and inflammation. Intra-articular injections of steroids are not totally harmless and other treatments must be preferred.


Asunto(s)
Cortisona/efectos adversos , Osteoartritis/inducido químicamente , Trastornos de la Articulación Temporomandibular/inducido químicamente , Cortisona/administración & dosificación , Femenino , Humanos , Inyecciones Intraarticulares/efectos adversos , Persona de Mediana Edad , Osteoartritis/diagnóstico , Articulación Temporomandibular/efectos de los fármacos , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/diagnóstico , Síndrome de la Disfunción de Articulación Temporomandibular/tratamiento farmacológico
9.
Equine Vet J ; 48(4): 523-7, 2016 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25891835

RESUMEN

REASONS FOR PERFORMING STUDY: Diseases of the temporomandibular joint (TMJ) are well reported in man and some domestic animals other than the horse. The pathophysiology of equine TMJ disease and the effects of disease on the kinematics of mastication are unknown. OBJECTIVES: To determine whether transient unilateral inflammation of the equine TMJ results in alterations in the masticatory cycle. STUDY DESIGN: An experimental controlled study utilising 6 horses of various ages with normal dentition. METHODS: Each horse was equipped with an optical motion tracking (kinematic) system. Horses were observed chewing grass hay over 3 min intervals. Regardless of the initial side of the power stroke in the masticatory cycle, lipopolysaccharide (LPS) was injected in the left TMJ in each horse and the horses were reassessed after 6 h. RESULTS: Four horses developed effusion of the injected TMJs; 2 of these also began quidding. All horses injected on the original side of the power stroke switched sides while the 2 injected on the contralateral side did not. All horses developed reduced vertical pitch (vertical opening) of the mandible. Overall, rostrocaudal movement of the mandible did not change; however, the timing of this movement relative to the phase of the masticatory cycle did. Injection with LPS did not affect the amount of lateral movement of the mandible. CONCLUSIONS: Injection of LPS into the TMJ significantly altered the masticatory cycle compared with baseline values representing avoidance behaviour due to inflammation of the joint, despite which the horses continued to eat using the contralateral mandible. Lipopolysaccharide administration also led to quidding and a loss of feed efficiency (in some individuals).


Asunto(s)
Masticación/fisiología , Trastornos de la Articulación Temporomandibular/veterinaria , Articulación Temporomandibular/fisiología , Animales , Fenómenos Biomecánicos , Enfermedades de los Caballos , Caballos , Inflamación , Lipopolisacáridos/toxicidad , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/patología
10.
J Korean Med Sci ; 30(5): 552-8, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25931785

RESUMEN

Temporomandibular joint (TMJ) disorder is clinically important because of its prevalence, chronicity, and therapy-refractoriness of the pain. In this study, we investigated the effect of infliximab in a mouse model of TMJ pain using a specially-engineered transducer for evaluating the changes in bite force (BF). The mice were randomly divided into three groups (7 mice per group): the control group, the complete Freund's adjuvant (CFA) group, and the infliximab group. BF was measured at day 0 (baseline BF). After measuring the baseline BF, CFA or incomplete Freund's adjuvant was injected into both TMJs and then the changes in BF were measured at days 1, 3, 5, 7, 9, and 13 after the TMJ injection. For measuring the BF, we used a custom-built BF transducer. Control, CFA, and infliximab groups showed similar baseline BF at day 0. From day 1, a significant reduction in BF was observed in the CFA group, and this reduction in BF was statistically significant compared to that in the control group (P < 0.05). This reduction in BF was maintained until day 7, and BF started to recover gradually from day 9. In the infliximab group also, the reduction in BF was observed on day 1, and this reduction was maintained until day 7. However, the degree of reduction in BF was less remarkable compared to that in the CFA group. The reduction in BF caused by injection of CFA into the TMJ could be partially alleviated by the injection of anti-tumor necrosis factor alpha, infliximab.


Asunto(s)
Antirreumáticos/uso terapéutico , Fuerza de la Mordida , Infliximab/uso terapéutico , Trastornos de la Articulación Temporomandibular/tratamiento farmacológico , Animales , Modelos Animales de Enfermedad , Adyuvante de Freund/toxicidad , Masculino , Ratones , Ratones Endogámicos ICR , Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/patología , Factores de Tiempo
11.
J Oral Facial Pain Headache ; 29(1): 31-40, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25635958

RESUMEN

AIMS: To test the hypothesis that experimental pain in the masseter muscle or temporomandibular joint (TMJ) will decrease the anterior maximum voluntary bite force (MVBF) and jaw muscle activity in relation to the perceived effort. METHODS: Sixteen volunteers participated in two experimental sessions. Participants were injected with 0.2 mL of monosodium glutamate (1.0 M) into either the masseter muscle or TMJ. The MVBF and corresponding electromyographic (EMG) activity of the masseter, anterior temporalis, and digastric muscles were recorded 10 times at an interval of 2 minutes before and after injection. Pain was measured using a visual analog scale and McGill Pain Questionnaire. In addition, participants were asked how they perceived the interference of pain on their biting performance. The data analysis included a two-way analysis of variance model and t test. RESULTS: There was no significant difference in peak pain intensity (P = .066) and duration of pain (P = .608) between painful muscle and TMJ injections, but TMJ injection produced a significantly larger area under the curve (P = .005) and a significantly higher pain rating index (P = .030). Pain in the muscle (P = .421) and TMJ (P = .057) did not significantly change the MVBF from baseline levels. The EMG activity also did not differ significantly from baseline levels during muscle pain. However, there was a significant increase (P = .028) in the EMG activity of the anterior temporalis and a significant decrease (P = .010) in the EMG activity of the anterior digastric muscle compared to baseline during TMJ pain. Subject-based reports also revealed that in the majority of cases (62.5%), pain did not interfere with the MVBF task. CONCLUSION: Experimental pain from either masseter muscle or TMJ did not affect the MVBF, in accordance with the subject-based reports. Jaw muscle activity, except for EMG activity of the anterior temporalis and anterior digastric muscles during TMJ pain, also remained unaffected by pain. The findings suggest that it is not pain in itself but rather how pain is perceived that may lead to adaptation of motor function, supporting an integrated pain adaptation model.


Asunto(s)
Fuerza de la Mordida , Dolor Facial/fisiopatología , Músculo Masetero/fisiopatología , Mialgia/fisiopatología , Trastornos de la Articulación Temporomandibular/fisiopatología , Adolescente , Adulto , Electromiografía/métodos , Dolor Facial/inducido químicamente , Femenino , Humanos , Inyecciones Intraarticulares , Inyecciones Intramusculares , Masculino , Mialgia/inducido químicamente , Músculos del Cuello/fisiopatología , Dimensión del Dolor/métodos , Percepción del Dolor/fisiología , Glutamato de Sodio/administración & dosificación , Glutamato de Sodio/efectos adversos , Músculo Temporal/fisiopatología , Trastornos de la Articulación Temporomandibular/inducido químicamente , Escala Visual Analógica , Adulto Joven
12.
Eur J Pain ; 18(9): 1280-9, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24715714

RESUMEN

BACKGROUND: We investigated both the efficacy and the sub-chronic toxicity of Tephrosia toxicaria Pers. in the zymosan-induced temporomandibular joint (TMJ) inflammatory hypernociception in rats evaluating the possible role of heme oxygenase-1 (HO-1). METHODS: Rats were pretreated with T. toxicaria (0.2, 2.0 or 20 mg/kg) 60 min before the intra-articular injection of zymosan (2 mg, 40 µL) in the left TMJ. In another series of experiments, rats were treated with ZnPP-IX (3 mg/kg), a specific HO-1 inhibitor, before T. toxicaria (20 mg/kg). Von Frey test was used to evaluate inflammatory hypernociception (g) 4 h after zymosan injection. Six hours after zymosan injection, the synovial lavage was collected for total cell count and myeloperoxidase (MPO) activity, and joint tissue for histopathological analysis and immunohistochemistry for HO-1. To evaluate the sub-chronic toxicity, mice received T. toxicaria (20 mg/kg) or saline once a day for 14 days to analyse body mass, organ weight and biochemical parameters. RESULTS: T. toxicaria partially reversed the zymosan-induced head withdrawal threshold, the number of cells and the MPO activity. T. toxicaria reduced the inflammatory cell influx in the synovial membrane. TMJ immunohistochemical analyses treated with T. toxicaria showed increased HO-1 expression. These effects of T. toxicaria were not observed in the presence of ZnPP-IX. T. toxicaria treatment for 14 days did not show significant signs of toxicity when administrated to mice. CONCLUSIONS: T. toxicaria did not produce any signs of toxicity and effectively decreased zymosan-induced TMJ inflammatory hypernociception dependent, at least in part, upon the HO-1 pathway integrity.


Asunto(s)
Hemo-Oxigenasa 1/metabolismo , Hiperalgesia/tratamiento farmacológico , Fitoterapia , Preparaciones de Plantas/farmacología , Trastornos de la Articulación Temporomandibular/tratamiento farmacológico , Tephrosia , Animales , Modelos Animales de Enfermedad , Inhibidores Enzimáticos/administración & dosificación , Inhibidores Enzimáticos/farmacología , Hemo-Oxigenasa 1/antagonistas & inhibidores , Hiperalgesia/inducido químicamente , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Masculino , Redes y Vías Metabólicas , Ratones , Preparaciones de Plantas/administración & dosificación , Preparaciones de Plantas/efectos adversos , Protoporfirinas/administración & dosificación , Protoporfirinas/farmacología , Ratas , Ratas Wistar , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/fisiopatología , Zimosan/farmacología
13.
Physiol Behav ; 125: 1-7, 2014 Feb 10.
Artículo en Inglés | MEDLINE | ID: mdl-24291383

RESUMEN

Temporomandibular disorder (TMD) is prevalent in dental clinics and can involve problems with the masticatory muscles or the temporomandibular joints (TMJ). The pain of TMD is frequently associated with inflammation in the TMJs, but it's etiology is considered to be multifactorial and includes biologic, behavioral, environmental, social, emotional and cognitive factors. The purpose of this investigation was to evaluate the anxiety-like behavior in rats exposed to temporomandibular inflammation via injection of Freund's Adjuvant (CFA) with the elevated plus maze (EPM) and light/dark box (LDB) tests and to evaluate nociceptive behavior with the von Frey test at different periods. Moreover, this study measured TMJ inflammation using plasma extravasation (Evans blue test) and the intraarticular infiltration of polymorphonuclear neutrophils (myeloperoxidase quantification). The results showed that rats that were submitted to TMJ inflammation exhibited a decreased number of entries into the open arms of the EPM and a decrease in the time spent in the light compartment and in the number of transitions in the LDB. Additionally, the number of entries in closed arms in the EPM, used as indicator of locomotor activity, did not alter between treatments. Furthermore, increases in mechanical sensitivity and increases in plasma extravasation in the joint tissue occurred throughout the inflammation process, along with an increase in myeloperoxidase in the synovial fluid of TMJ. Our results suggest that the temporomandibular inflammation induced by CFA produced anxiety-like behaviors in rats and induced nociceptive behavior across different periods of inflammation.


Asunto(s)
Ansiedad/psicología , Inflamación/patología , Inflamación/psicología , Dolor Nociceptivo/patología , Dolor Nociceptivo/psicología , Trastornos de la Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/psicología , Animales , Ansiedad/complicaciones , Conducta Animal , Extravasación de Materiales Terapéuticos y Diagnósticos/patología , Adyuvante de Freund , Inflamación/inducido químicamente , Inflamación/complicaciones , Masculino , Infiltración Neutrófila , Dolor Nociceptivo/inducido químicamente , Dolor Nociceptivo/complicaciones , Peroxidasa/metabolismo , Ratas , Líquido Sinovial/metabolismo , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/complicaciones , Factores de Tiempo
14.
Eur J Oral Sci ; 121(6): 573-83, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24206074

RESUMEN

Temporomandibular joint (TMJ) arthritis is a common cause of orofacial pain. In the present study, the modulatory effects of N-methyl-d-aspartate receptors (NMDA-Rs) and magnesium were investigated in TMJ arthritis hypernociception. Male Wistar rats received an intra-articular injection of carrageenan (Cg) in the TMJ, and mechanical hypernociception was measured. The NMDA-R antagonist, MK-801, and magnesium chloride (MgCl2 ) were administered before arthritis induction. Magnesium deficiency was promoted by feeding rats a synthetic magnesium-free diet for 9 d before injection of Cg. The Cg induced mechanical hypernociception that lasted for 120 h. MK-801 inhibited this hypernociceptive state. MgCl2 pretreatment prevented Cg-induced hypernociception and altered the nociceptive threshold in the absence of Cg. Magnesium deficiency increased hypernociception and induced spontaneous hypernociceptive behavior. TMJ arthritis increased the expression of mRNA for all NMDA-R subunits and immunostaining of phosphorylated NR1 (phospho-NR1). MgCl2 inhibited expression of NR2B mRNA and phospho-NR1 immunostaining and increased expression of NR3 mRNA. Magnesium deficiency increased expression of both NR1 and NR3 mRNAs and phospho-NR1 immunostaining in the trigeminal subnucleus caudalis. We found that magnesium modulates nociceptive behavior and induces NMDA-R subunit rearrangement in the subnucleus caudalis. The present results may lead to a better understanding of central processing in the nociceptive trigeminal pathway and the development of new approaches to treat orofacial pain with a TMJ origin.


Asunto(s)
Deficiencia de Magnesio/metabolismo , Magnesio/farmacología , Osteoartritis/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Trastornos de la Articulación Temporomandibular/metabolismo , Núcleo Caudal del Trigémino/metabolismo , Nervio Trigémino/efectos de los fármacos , Análisis de Varianza , Animales , Carragenina , Expresión Génica , Magnesio/sangre , Deficiencia de Magnesio/inducido químicamente , Masculino , Datos de Secuencia Molecular , Dolor Nociceptivo/metabolismo , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/tratamiento farmacológico , Factores de Tiempo , Nervio Trigémino/metabolismo
15.
J Dent Res ; 92(10): 918-24, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23934157

RESUMEN

Temporomandibular joint osteoarthritis (TMJOA) is clinically characterized by female preponderance, with a female-to-male ratio of more than 2:1; however, the underlying mechanism remains obscure. We examined the effects of estrogen on TMJOA induced by monosodium iodoacetate. Female rats were randomly and equally divided into 5 groups: control, sham-ovariectomized, and ovariectomized rats treated, respectively, with 17ß-estradiol (E2) at doses of 0 µg, 20 µg, and 80 µg/day until the end of the experiment. After induction of TMJOA, TMJs were evaluated by histopathology and microCT, and the expression of Fas, FasL, caspase 3, and caspase 8 was evaluated by real-time polymerase chain-reaction or immunohistochemistry. Another 5 groups of female rats were used to evaluate the effect of estrogen receptor antagonist ICI 182780 on E2 effects on TMJOA, when injected intraperitoneally into the control, sham-ovariectomized, and 80-µg-E2-treated groups. We found that E2 potentiated cartilage degradation and subchondral bone erosion in iodoacetate-induced TMJOA. E2 also potentiated mRNA expression of Fas, FasL, caspase 3, and caspase 8 in the condylar cartilage. Moreover, the estrogen receptor antagonist partially blocked E2 effects on TMJOA. These findings suggest that E2 could aggravate TMJOA, which may be an important mechanism underlying the sexual dimorphism of TMJOA.


Asunto(s)
Estradiol/metabolismo , Osteoartritis/metabolismo , Caracteres Sexuales , Trastornos de la Articulación Temporomandibular/metabolismo , Articulación Temporomandibular/metabolismo , Animales , Apoptosis/genética , Apoptosis/fisiología , Cartílago Articular/efectos de los fármacos , Cartílago Articular/metabolismo , Caspasa 3/metabolismo , Caspasa 8/metabolismo , Condrocitos/metabolismo , Estradiol/efectos adversos , Proteína Ligando Fas/metabolismo , Femenino , Ácido Yodoacético , Osteoartritis/inducido químicamente , Ovariectomía , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Articulación Temporomandibular/efectos de los fármacos , Trastornos de la Articulación Temporomandibular/inducido químicamente , Receptor fas/metabolismo
16.
Eur J Pain ; 17(2): 174-84, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22715057

RESUMEN

BACKGROUND: Previous studies have shown 17ß-estradiol will reduce temporomandibular joint (TMJ) inflammation and hypersensitivity in female rats. Although male rats contain significant amounts of oestradiol, it was unknown whether a physiological concentration of 17ß-estradiol would attenuate male TMJ inflammation and nociception. METHODS: Intact and castrated rats were given a physiological concentration of oestradiol to examine first, if oestradiol will affect male TMJ nociception/inflammation and, second, if administration of oestradiol would act synergistically with endogenous male hormones to attenuate TMJ nociception. The hormonally treated rats were given TMJ injections of complete Freund's adjuvant (CFA) and then nociception was measured using a validated method in which a lengthening in meal duration is directly correlated to the intensity of deep TMJ nociception. Inflammation was assayed by quantitating pro-inflammatory gene expression. RESULTS: Meal duration was significantly lengthened after TMJ CFA injection and this lengthening was significantly attenuated in the castrated but not intact males after administering a physiological concentration of oestradiol. A physiological concentration of 17ß-estradiol also significantly increased IL-6 expression in the inflamed TMJ of castrated males while 17ß-estradiol did not alter IL-1ß, CXCL2 and CCL20 expression. Castration increased pro-inflammatory mediators IL-6, IL-1ß and CXCL2 suggesting male sex hormones were anti-inflammatory. Calcitonin gene-related peptide in the trigeminal ganglia was unchanged. CONCLUSIONS: Similar to females, male rats with TMJ inflammation showed a reduced nociceptive response after treatment with a physiological concentration of oestradiol suggesting the effects of oestradiol treatment were not constrained by organizational processes in the males.


Asunto(s)
Antiinflamatorios , Estradiol/uso terapéutico , Trastornos de la Articulación Temporomandibular/tratamiento farmacológico , Animales , Quimiocinas/metabolismo , Implantes de Medicamentos , Estradiol/sangre , Ciclo Estral/efectos de los fármacos , Conducta Alimentaria/efectos de los fármacos , Femenino , Adyuvante de Freund , Masculino , Nocicepción/efectos de los fármacos , Orquiectomía , Dimensión del Dolor/efectos de los fármacos , Proestro/fisiología , Ratas , Ratas Sprague-Dawley , Receptores de Péptido Relacionado con el Gen de Calcitonina/metabolismo , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/patología
17.
Pain Med ; 13(12): 1590-600, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23110394

RESUMEN

OBJECTIVE: To examine the hypothesis that glial activation would regulate the expression of the N-methyl-D-aspartate receptor subunit 1 (NR1) in the trigeminal subnucleus caudalis (Sp5C) after temporomandibular joint (TMJ) inflammation. METHODS: Inflammation of TMJ was produced in rats by injecting 50 µL complete Freund's adjuvant (CFA) into unilateral TMJ space. Sham control rats received incomplete Freund's adjuvant injection. Mechanical nociception in the affected and non-affected TMJ site was tested by using a digital algometer. Fractalkine, fluorocitrate, and/or MK801 were intracisternally administrated to examine the relationship between astroglial activation and NR1 upregulation. RESULTS: CFA TMJ injection resulted in persistent ipsilateral mechanical hyperalgesia 1, 3, and 5 days after CFA injection. The inflammation also induced significant upregulation of CX3C chemokine receptor 1 and glial fibrillary acidic protein (GFAP) beginning on day 1 and of NR1 beginning on day 3 within the ipsilateral Sp5C. Intracisternal administration of fluorocitrate for 5 days blocked the development of mechanical hyperalgesia as well as the upregulation of GFAP and NR1 in the Sp5C. Conversely, intracisternal injection of fractalkine for 5 days exacerbated the expression of NR1 in Sp5C and mechanical hyperalgesia induced by TMJ inflammation. Moreover, once daily intracisternal fractalkine administration for 5 days in naïve rats induced the upregulation of NR1 and mechanical hyperalgesia. CONCLUSIONS: These results suggest that astroglial activation contributes to the mechanism of TMJ pain through the regulation of NR1 expression in Sp5C.


Asunto(s)
Astrocitos/metabolismo , Hiperalgesia/metabolismo , Inflamación/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Trastornos de la Articulación Temporomandibular/metabolismo , Núcleo Caudal del Trigémino/metabolismo , Adyuvantes Inmunológicos , Animales , Receptor 1 de Quimiocinas CX3C , Modelos Animales de Enfermedad , Adyuvante de Freund , Proteína Ácida Fibrilar de la Glía/metabolismo , Hiperalgesia/fisiopatología , Inflamación/inducido químicamente , Inflamación/fisiopatología , Masculino , Dimensión del Dolor , Estimulación Física , Ratas , Ratas Sprague-Dawley , Receptores de Quimiocina/metabolismo , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/fisiopatología , Núcleo Caudal del Trigémino/fisiopatología , Regulación hacia Arriba
19.
Neurosci Lett ; 528(2): 126-30, 2012 Oct 24.
Artículo en Inglés | MEDLINE | ID: mdl-22975136

RESUMEN

To determine whether the vestibular nuclei are affected by inflammation of temporomandibular joint (TMJ) region, we studied vestibular nucleus neural activity using two experimental groups: (1) normal saline 0.1cm(3) injection at right TMJ region, (2) 10% formalin 0.1cm(3) injection at right TMJ region. Neural activity after 24 hours was assessed by immunohistochemical staining with free-floating section at the level of interaural -1.30 mm to -2.00 mm for c-Fos. In inflammation group, formalin injection produced a bilateral increase in c-Fos at vestibular nucleus with ipsilesional side higher activity. In control group, expression of c-Fos protein was also observed in the vestibular nucleus (VN), especially MVN. But stain intensity of Fos-positive neurons was much weaker and mean number of c-Fos positive cells was fewer than inflammation group. This result suggests that there is a close neural connection between TMJ and vestibular nucleus, especially in case of inflammation.


Asunto(s)
Trastornos de la Articulación Temporomandibular/metabolismo , Articulación Temporomandibular/inmunología , Núcleos Vestibulares/metabolismo , Animales , Formaldehído , Inmunohistoquímica , Inflamación/inducido químicamente , Inflamación/inmunología , Masculino , Neuronas/metabolismo , Proteínas Proto-Oncogénicas c-fos/metabolismo , Ratas , Ratas Sprague-Dawley , Trastornos de la Articulación Temporomandibular/inducido químicamente , Trastornos de la Articulación Temporomandibular/inmunología
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