Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 524
Filtrar
1.
PLoS One ; 15(2): e0220753, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32027652

RESUMEN

BACKGROUND: Three arthropod-borne viruses (arboviruses) causing human disease have been the focus of a large number of studies in the Americas since 2013 due to their global spread and epidemiological impacts: Zika, dengue, and chikungunya viruses. A large proportion of infections by these viruses are asymptomatic. However, all three viruses are associated with moderate to severe health consequences in a small proportion of cases. Two mosquito species, Aedes aegypti and Aedes albopictus, are among the world's most prominent arboviral vectors, and are known vectors for all three viruses in the Americas. OBJECTIVES: This review summarizes the state of the entomological literature surrounding the mosquito vectors of Zika, dengue and chikungunya viruses and factors affecting virus transmission. The rationale of the review was to identify and characterize entomological studies that have been conducted in the Americas since the introduction of chikungunya virus in 2013, encompassing a period of arbovirus co-circulation, and guide future research based on identified knowledge gaps. METHODS: The preliminary search for this review was conducted on PubMed (National Library of Health, Bethesda, MD, United States). The search included the terms 'zika' OR 'dengue' OR 'chikungunya' AND 'vector' OR 'Aedes aegypti' OR 'Aedes albopictus'. The search was conducted on March 1st of 2018, and included all studies since January 1st of 2013. RESULTS: A total of 96 studies were included in the scoping review after initial screening and subsequent exclusion of out-of-scope studies, secondary data publications, and studies unavailable in English language. KEY FINDINGS: We observed a steady increase in number of publications, from 2013 to 2018, with half of all studies published from January 2017 to March 2018. Interestingly, information on Zika virus vector species composition was abundant, but sparse on Zika virus transmission dynamics. Few studies examined natural infection rates of Zika virus, vertical transmission, or co-infection with other viruses. This is in contrast to the wealth of research available on natural infection and co-infection for dengue and chikungunya viruses, although vertical transmission research was sparse for all three viruses.


Asunto(s)
Infecciones por Arbovirus/transmisión , Arbovirus/patogenicidad , Mosquitos Vectores/virología , Américas/epidemiología , Virus Chikungunya/patogenicidad , Virus del Dengue/patogenicidad , Humanos , Virus Zika/patogenicidad
2.
PLoS One ; 15(1): e0227058, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31910225

RESUMEN

Nanotrap® (NT) particles are hydrogel microspheres developed for target analyte separation and discovery applications. NT particles consist of cross-linked N-isopropylacrylamide (NIPAm) copolymers that are functionalized with a variety of chemical affinity baits to enable broad-spectrum collection and retention of target proteins, nucleic acids, and pathogens. NT particles have been previously shown to capture and enrich arboviruses including Rift Valley fever and Venezuelan equine encephalitis viruses. Yet, there is still a need to enhance the detection ability for other re-emerging viruses such as Zika (ZIKV), chikungunya (CHIKV), and dengue (DENV) viruses. In this study, we exploited NT particles with different affinity baits, including cibacron blue, acrylic acid, and reactive red 120, to evaluate their capturing and enrichment capability for ZIKV, DENV and CHIKV in human fluids. Our results demonstrate that CN1030, a NT particle conjugated with reactive red 120, can recover between 8-16-fold greater genomic copies of ZIKV, CHIKV and DENV in virus spiked urine samples via RT-qPCR, superior to the other chemical baits. Also, we observed that CN1030 simultaneously enriched ZIKV, CHIKV and DENV in co-infection-based settings and could stabilize ZIKV, but not CHIKV infectivity in saliva spiked samples. CN1030 enriched viral detection at various viral concentrations, with significant enhancement observed at viral titers as low as 100 PFU/mL for ZIKV and 10 PFU/mL for CHIKV. The detection of ZIKV was further enhanced with NT particles by processing of larger volume urine samples. Furthermore, we developed a magnetic NT particle, CN3080, based on the same backbone of CN1030, and demonstrated that CN3080 could also capture and enrich ZIKV and CHIKV in a dose-dependent manner. Finally, in silico docking predictions support that the affinity between reactive red 120 and ZIKV or CHIKV envelope proteins appeared to be greater than acrylic acid. Overall, our data show that NT particles along with reactive red 120 can be utilized as a pre-processing technology for enhancement of detecting febrile-illness causing viruses.


Asunto(s)
Infecciones por Arbovirus/orina , Virus Chikungunya/aislamiento & purificación , Virus del Dengue/aislamiento & purificación , Técnicas de Diagnóstico Molecular/métodos , Nanopartículas/química , Virus Zika/aislamiento & purificación , Infecciones por Arbovirus/diagnóstico , Infecciones por Arbovirus/virología , Virus Chikungunya/genética , Virus Chikungunya/patogenicidad , Colorantes/química , Virus del Dengue/genética , Virus del Dengue/patogenicidad , Humanos , Hidrogeles/química , Nanopartículas/metabolismo , Reacción en Cadena de la Polimerasa/métodos , Unión Proteica , Saliva/virología , Orina/virología , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/metabolismo , Virus Zika/genética , Virus Zika/patogenicidad
3.
PLoS One ; 15(1): e0227676, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31935257

RESUMEN

Zika virus infection during pregnancy is associated with miscarriage and with a broad spectrum of fetal and neonatal developmental abnormalities collectively known as congenital Zika syndrome (CZS). Symptomology of CZS includes malformations of the brain and skull, neurodevelopmental delay, seizures, joint contractures, hearing loss and visual impairment. Previous studies of Zika virus in pregnant rhesus macaques (Macaca mulatta) have described injury to the developing fetus and pregnancy loss, but neonatal outcomes following fetal Zika virus exposure have yet to be characterized in nonhuman primates. Herein we describe the presentation of rhesus macaque neonates with a spectrum of clinical outcomes, including one infant with CZS-like symptoms including cardiomyopathy, motor delay and seizure activity following maternal infection with Zika virus during the first trimester of pregnancy. Further characterization of this neonatal nonhuman primate model of gestational Zika virus infection will provide opportunities to evaluate the efficacy of pre- and postnatal therapeutics for gestational Zika virus infection and CZS.


Asunto(s)
Modelos Animales de Enfermedad , Infección por el Virus Zika/veterinaria , Virus Zika/patogenicidad , Animales , Cardiomiopatías/virología , Femenino , Feto/virología , Macaca mulatta , Microcefalia/virología , Embarazo , Complicaciones Infecciosas del Embarazo/veterinaria , Complicaciones Infecciosas del Embarazo/virología , Primer Trimestre del Embarazo , Convulsiones/virología , Infección por el Virus Zika/virología
4.
Nat Commun ; 10(1): 5730, 2019 12 16.
Artículo en Inglés | MEDLINE | ID: mdl-31844054

RESUMEN

In 2015 and 2016, Zika virus (ZIKV) swept through dengue virus (DENV) endemic areas of Latin America. These viruses are of the same family, share a vector and may interact competitively or synergistically through human immune responses. We examine dengue incidence from Brazil and Colombia before, during, and after the Zika epidemic. We find evidence that dengue incidence was atypically low in 2017 in both countries. We investigate whether subnational Zika incidence is associated with changes in dengue incidence and find mixed results. Using simulations with multiple assumptions of interactions between DENV and ZIKV, we find cross-protection suppresses incidence of dengue following Zika outbreaks and low periods of dengue incidence are followed by resurgence. Our simulations suggest correlations in DENV and ZIKV reproduction numbers could complicate associations between ZIKV incidence and post-ZIKV DENV incidence and that periods of low dengue incidence are followed by large increases in dengue incidence.


Asunto(s)
Dengue/epidemiología , Brotes de Enfermedades/estadística & datos numéricos , Enfermedades Endémicas/estadística & datos numéricos , Infección por el Virus Zika/epidemiología , Anticuerpos Antivirales/inmunología , Brasil/epidemiología , Colombia/epidemiología , Reacciones Cruzadas/inmunología , Dengue/inmunología , Dengue/virología , Virus del Dengue/inmunología , Virus del Dengue/patogenicidad , Monitoreo Epidemiológico , Humanos , Incidencia , Modelos de Interacción Espacial , Análisis de Regresión , Virus Zika/inmunología , Virus Zika/patogenicidad , Infección por el Virus Zika/inmunología , Infección por el Virus Zika/virología
5.
Nat Commun ; 10(1): 4430, 2019 09 27.
Artículo en Inglés | MEDLINE | ID: mdl-31562326

RESUMEN

Zika virus (ZIKV) invades and persists in the central nervous system (CNS), causing severe neurological diseases. However the virus journey, from the bloodstream to tissues through a mature endothelium, remains unclear. Here, we show that ZIKV-infected monocytes represent suitable carriers for viral dissemination to the CNS using human primary monocytes, cerebral organoids derived from embryonic stem cells, organotypic mouse cerebellar slices, a xenotypic human-zebrafish model, and human fetus brain samples. We find that ZIKV-exposed monocytes exhibit higher expression of adhesion molecules, and higher abilities to attach onto the vessel wall and transmigrate across endothelia. This phenotype is associated to enhanced monocyte-mediated ZIKV dissemination to neural cells. Together, our data show that ZIKV manipulates the monocyte adhesive properties and enhances monocyte transmigration and viral dissemination to neural cells. Monocyte transmigration may represent an important mechanism required for viral tissue invasion and persistence that could be specifically targeted for therapeutic intervention.


Asunto(s)
Moléculas de Adhesión Celular/metabolismo , Monocitos/metabolismo , Monocitos/virología , Neuronas/metabolismo , Migración Transendotelial y Transepitelial/fisiología , Infección por el Virus Zika/metabolismo , Virus Zika/fisiología , Virus Zika/patogenicidad , Animales , Adhesión Celular/fisiología , Supervivencia Celular , Sistema Nervioso Central/metabolismo , Sistema Nervioso Central/patología , Sistema Nervioso Central/virología , Cerebelo/patología , Cerebelo/virología , Modelos Animales de Enfermedad , Células Madre Embrionarias , Endotelio/virología , Femenino , Humanos , Monocitos/patología , Neuronas/patología , Neuronas/virología , Organoides/metabolismo , Organoides/patología , Pez Cebra , Infección por el Virus Zika/patología , Infección por el Virus Zika/virología
6.
Nat Commun ; 10(1): 4155, 2019 09 13.
Artículo en Inglés | MEDLINE | ID: mdl-31519912

RESUMEN

Zika virus (ZIKV) infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the underlying mechanisms remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pregnancy, at pre- and peri-implantation, because most current ZIKV pregnancy studies have focused on post-implantation stages. Here, we demonstrate that trophectoderm cells of pre-implantation human and mouse embryos can be infected with ZIKV, and propagate virus causing neural progenitor cell death. These findings are corroborated by the dose-dependent nature of ZIKV susceptibility of hESC-derived trophectoderm cells. Single blastocyst RNA-seq reveals key transcriptional changes upon ZIKV infection, including nervous system development, prior to commitment to the neural lineage. The pregnancy rate of mice is >50% lower in pre-implantation infection than infection at E4.5, demonstrating that pre-implantation ZIKV infection leads to miscarriage. Cumulatively, these data elucidate a previously unappreciated association of pre- and peri-implantation ZIKV infection and microcephaly.


Asunto(s)
Complicaciones Infecciosas del Embarazo/metabolismo , Infección por el Virus Zika/complicaciones , Infección por el Virus Zika/metabolismo , Virus Zika/patogenicidad , Aborto Espontáneo/metabolismo , Aborto Espontáneo/fisiopatología , Animales , Blastocisto/citología , Blastocisto/metabolismo , Implantación del Embrión/fisiología , Femenino , Desarrollo Fetal/genética , Desarrollo Fetal/fisiología , Humanos , Ratones , Ratones Endogámicos C57BL , Embarazo , ARN Viral/genética , Investigación en Medicina Traslacional/métodos , Trofoblastos/citología , Trofoblastos/metabolismo
7.
Nucleic Acids Res ; 47(18): 9789-9802, 2019 10 10.
Artículo en Inglés | MEDLINE | ID: mdl-31392996

RESUMEN

Members of the Flaviviridae family, including dengue virus (DENV) and yellow fever virus, cause serious disease in humans, whilst maternal infection with Zika virus (ZIKV) can induce microcephaly in newborns. Following infection, flaviviral RNA genomes are translated to produce the viral replication machinery but must then serve as a template for the transcription of new genomes. However, the ribosome and viral polymerase proceed in opposite directions along the RNA, risking collisions and abortive replication. Whilst generally linear, flavivirus genomes can adopt a circular conformation facilitated by long-range RNA-RNA interactions, shown to be essential for replication. Using an in vitro reconstitution approach, we demonstrate that circularization inhibits de novo translation initiation on ZIKV and DENV RNA, whilst the linear conformation is translation-competent. Our results provide a mechanism to clear the viral RNA of ribosomes in order to promote efficient replication and, therefore, define opposing roles for linear and circular conformations of the flavivirus genome.


Asunto(s)
Virus del Dengue/genética , Flavivirus/genética , Biosíntesis de Proteínas , Virus Zika/genética , Virus del Dengue/patogenicidad , Flavivirus/patogenicidad , Genoma Viral/genética , Genómica , Humanos , Recién Nacido , ARN Viral/genética , Replicación Viral/genética , Virus de la Fiebre Amarilla/genética , Virus de la Fiebre Amarilla/patogenicidad , Virus Zika/patogenicidad , Infección por el Virus Zika/genética , Infección por el Virus Zika/virología
8.
Cells ; 8(7)2019 07 15.
Artículo en Inglés | MEDLINE | ID: mdl-31311201

RESUMEN

Zika virus (ZIKV) has emerged as an important human pathogen that can cause congenital defects in the fetus and neurological conditions in adults. The interferon (IFN) system has proven crucial in restricting ZIKV replication and pathogenesis. The canonical IFN response is triggered by the detection of viral RNA through RIG-I like receptors followed by activation of the adaptor protein MAVS on mitochondrial membranes. Recent studies have shown that a second organelle, peroxisomes, also function as a signaling platforms for the IFN response. Here, we investigated how ZIKV infection affects peroxisome biogenesis and antiviral signaling. We show that ZIKV infection depletes peroxisomes in human fetal astrocytes, a brain cell type that can support persistent infection. The peroxisome biogenesis factor PEX11B was shown to inhibit ZIKV replication, likely by increasing peroxisome numbers and enhancing downstream IFN-dependent antiviral signaling. Given that peroxisomes play critical roles in brain development and nerve function, our studies provide important insights into the roles of peroxisomes in regulating ZIKV infection and potentially neuropathogenesis.


Asunto(s)
Interacciones Huésped-Patógeno , Peroxisomas/virología , Virus Zika/patogenicidad , Animales , Astrocitos/inmunología , Astrocitos/virología , Línea Celular Tumoral , Células Cultivadas , Células HEK293 , Humanos , Inmunidad Innata , Interferones/metabolismo , Proteínas de la Membrana/metabolismo , Transducción de Señal , Células Vero , Replicación Viral , Virus Zika/fisiología
9.
Chin Med J (Engl) ; 132(14): 1645-1653, 2019 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-31268910

RESUMEN

BACKGROUND: Zika virus (ZIKV) has emerged as a global pathogen causing significant public health concerns. China has reported several imported cases where ZIKV were carried by travelers who frequently travel between China and ZIKV-endemic regions. To fully characterize the ZIKV strains isolated from the cases reported in China and assess the risk of ZIKV transmission in China, comprehensive phylogenetic and genetic analyses were performed both on all ZIKV sequences of China and on a group of scientifically selected ZIKV sequences reported in some of the top interested destinations for Chinese travelers. METHODS: ZIKV genomic sequences were retrieved from the National Center for Biotechnology Information database through stratified sampling. Recombination event detection, maximum likelihood (ML) phylogenetic analysis, molecular clock analysis, selection pressure analysis, and amino acid substitution analysis were used to reconstruct the epidemiology and molecular transmission of ZIKV. RESULTS: The present study investigated 18 ZIKV sequences from China and 70 sequences from 16 selected countries. Recombination events rarely happens in all ZIKV Asian lineage. ZIKV genomes were generally undergone episodic positive selection (17 sites), and only one site was under pervasive positive selection. All ZIKV imported into China were Asian lineage and were assigned into two clusters: Venezuela-origin (cluster A) and Samoa-origin cluster (cluster B) with common ancestor from French Polynesia. The time of most recent common ancestors of Cluster A dated to approximately 2013/11 (95% highest posterior density [HPD] 2013/06, 2014/03) and cluster B dated to 2014/08 (95% HPD 2014/02, 2015/01). Cluster B is more variable than Cluster A in comparison with other clusters, but no varied site of biological significance was revealed. ZIKV strains in Southeast Asia countries are independent from strains in America epidemics. CONCLUSIONS: The genetic evolution of ZIKV is conservative. There are two independent introductions of ZIKV into China and China is in danger of autochthonous transmission of ZIKV because of high-risk surrounding areas. Southeast Asia areas have high risk of originating the next large-scale epidemic ZIKV strains.


Asunto(s)
Proteínas no Estructurales Virales/metabolismo , Infección por el Virus Zika/genética , Virus Zika/patogenicidad , China , Evolución Molecular , Genoma Viral/genética , Funciones de Verosimilitud , Filogenia , Estructura Secundaria de Proteína , Medición de Riesgo , Proteínas no Estructurales Virales/química , Proteínas no Estructurales Virales/genética , Virus Zika/genética , Infección por el Virus Zika/transmisión
10.
BMC Res Notes ; 12(1): 326, 2019 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-31182146

RESUMEN

OBJECTIVE: Increase in the evidence of global occurrence of Zika viral infection suggests that in Africa the circulation of the virus which causes 80% of asymptomatic infection could be undetected and/or overlooked. We sought to serologically detect Zika virus infection in febrile patients at Greater Accra Regional Hospital, Ghana. RESULTS: Of the 160 patient serum samples analyzed, 33 were found to have antibodies against Zika virus infection. Among the sero-positives 30 (91%) of the cases were anti-Zika virus IgM with the 21-30-year age group recording the highest number of 8 (26%) and 2 (7%) cases being the least for the 61 years and above age group. All sero-positive febrile patients developed at least one symptom consistent with Zika virus infection: 33 (100%) fever, 25 (76%) muscle pain, 24 (73%) joint pain, and conjunctivitis 2 (6%). Digestive symptoms recorded include 16 (49%) nausea, 12 (36%) vomiting and diarrhea 18 (55%). In addition, 28 (85%) loss of appetite, 14 (75%) rapid respiration and chest pain 15 (42%) were reported by seropositive febrile patients. Our data indicates exposure to Zika virus which suggests the possible circulation of the virus among febrile patients in Ghana with a sero-prevalence rate of 20.6%.


Asunto(s)
Anticuerpos Antivirales/sangre , Artralgia/inmunología , Fiebre/inmunología , Mialgia/inmunología , Infección por el Virus Zika/inmunología , Virus Zika/inmunología , Adolescente , Adulto , Anciano , Artralgia/diagnóstico , Artralgia/epidemiología , Artralgia/fisiopatología , Niño , Preescolar , Conjuntivitis Viral/diagnóstico , Conjuntivitis Viral/epidemiología , Conjuntivitis Viral/inmunología , Conjuntivitis Viral/fisiopatología , Estudios Transversales , Diarrea/diagnóstico , Diarrea/epidemiología , Diarrea/inmunología , Diarrea/fisiopatología , Femenino , Fiebre/diagnóstico , Fiebre/epidemiología , Fiebre/fisiopatología , Ghana/epidemiología , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Masculino , Persona de Mediana Edad , Mialgia/diagnóstico , Mialgia/epidemiología , Mialgia/fisiopatología , Náusea/diagnóstico , Náusea/epidemiología , Náusea/inmunología , Náusea/fisiopatología , Estudios Seroepidemiológicos , Vómitos/diagnóstico , Vómitos/epidemiología , Vómitos/inmunología , Vómitos/fisiopatología , Virus Zika/crecimiento & desarrollo , Virus Zika/patogenicidad , Infección por el Virus Zika/diagnóstico , Infección por el Virus Zika/epidemiología , Infección por el Virus Zika/fisiopatología
11.
Microbes Infect ; 21(8-9): 353-360, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31158508

RESUMEN

Since the ZIKV outbreak in Brazil in 2015, the scientific community has joined efforts to gather more information on the epidemiology, clinical features and pathogenicity of the virus. Here, we summarize the most important advances made recently and discuss promising, innovative approaches to understand and control ZIKV infection.


Asunto(s)
Infección por el Virus Zika/prevención & control , Infección por el Virus Zika/virología , Virus Zika/fisiología , Virus Zika/patogenicidad , Animales , Antivirales/farmacología , Arbovirus/patogenicidad , Arbovirus/fisiología , Brasil/epidemiología , Humanos , Inmunidad Innata , Mosquitos Vectores/virología , Vacunas Virales/inmunología , Virus Zika/efectos de los fármacos , Infección por el Virus Zika/epidemiología , Infección por el Virus Zika/inmunología
12.
J Assist Reprod Genet ; 36(7): 1339-1349, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31147867

RESUMEN

Zika virus (ZIKV) is mainly transmitted through Aedes mosquito bites, but sexual and post-transfusion transmissions have been reported. During acute infection, ZIKV is detectable in most organs and body fluids including human semen. Although it is not currently epidemic, there is a concern that the virus can still reemerge since the male genital tract might harbor persistent reservoirs that could facilitate viral transmission over extended periods, raising concerns among public health and assisted reproductive technologies (ART) experts and professionals. So far, the consensus is that ZIKV infection in the testes or epididymis might affect sperm development and, consequently, male fertility. Still, diagnostic tests have not yet been adapted to resource-restricted countries. This manuscript provides an updated overview of the cellular and molecular mechanisms of ZIKV infection and reviews data on ZIKV persistence in semen and associated risks to the male reproductive system described in human and animal models studies. We provide an updated summary of the impact of the recent ZIKV outbreak on human-ART, weighing on current recommendations and diagnostic approaches, both available and prospective, with special emphasis on mass spectrometry-based biomarker discovery. In the light of the identified gaps in our accumulated knowledge on the subject, we highlight the importance for couples seeking ART to follow the constantly revised guidelines and the need of specific ZIKV diagnosis tools for semen screening to contain ZIKV virus spread and make ART safer.


Asunto(s)
Biomarcadores , Genitales Masculinos/fisiopatología , Infección por el Virus Zika/fisiopatología , Virus Zika/patogenicidad , Animales , Femenino , Genitales Masculinos/virología , Humanos , Masculino , Espectrometría de Masas , Modelos Animales , Semen/metabolismo , Infección por el Virus Zika/virología
13.
PLoS Negl Trop Dis ; 13(6): e0007387, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-31170143

RESUMEN

We investigated the growth properties and virulence in mice of three Zika virus (ZIKV) strains of Asian/American lineage, PRVABC59, ZIKV/Hu/Chiba/S36/2016 (ChibaS36), and ZIKV/Hu/NIID123/2016 (NIID123), belonging to the three distinct subtypes of this lineage. The American-subtype strain, PRVABC59, showed the highest growth potential in vitro, whereas the Southeast Asian-subtype strain, NIID123, showed the lowest proliferative capacity. Moreover, PRVABC59- and NIID123-infected mice showed the highest and lowest viremia levels and infectious virus levels in the testis, respectively, and the rate of damaged testis in PRVABC59-infected mice was higher than in mice infected with the other two strains. Lastly, ZIKV NS1 antigen was detected in the damaged testes of mice infected with PRVABC59 and the Pacific-subtype strain, ChibaS36, at 2 weeks post-inoculation and in the epididymides of PRVABC59-infected mice at 6 weeks post-inoculation. Our results indicate that PRVABC59 and ChibaS36 exhibit increased abilities to grow in vitro and in vivo and to induce testis damage in mice.


Asunto(s)
Infección por el Virus Zika/virología , Virus Zika/crecimiento & desarrollo , Animales , Sangre/virología , Modelos Animales de Enfermedad , Epidídimo/virología , Masculino , Ratones Endogámicos C57BL , Testículo/virología , Carga Viral , Virulencia , Virus Zika/aislamiento & purificación , Virus Zika/patogenicidad
14.
Matern Child Health J ; 23(8): 1140-1145, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31209616

RESUMEN

OBJECTIVES: This study sought to describe the knowledge and perceptions of pregnant women in Miami-Dade County concerning Zika virus (ZIKV) in their community, to characterize their testing behaviors, and to identify any barriers that would keep them from seeking testing. METHODS: The Florida Department of Health in Miami-Dade County partnered with the Healthy Start Coalition of Miami-Dade to administer an assessment survey in eight OBGYN clinics from June to August 2017. The survey captured past ZIKV testing practices, attitudes towards testing, barriers to testing, risk perception of ZIKV in the participants' community, and ZIKV-related knowledge. Descriptive analyses were performed on variables of interest. Chi squared tests examined associations between categorical variables. RESULTS: A total of 363 participants were included in the analysis. Of these, 203 (55.9%) thought they should be tested for ZIKV, and less than half of the participants reported having been previously tested (152, 41.9%). Participants with some high school education were significantly more likely than those with higher education levels to see ZIKV as a "big problem" in the community (p = 0.0026). There was a significant association (p ≤ 0.0001) between women who thought that they should be tested, and those who perceived ZIKV to be a medium or big problem in their community. CONCLUSIONS FOR PRACTICE: Health interventions that focus on increasing ZIKV knowledge should also place greater emphasis on risk communication when targeting the pregnant population. Having a higher risk perception may be more predictive of testing behaviors than having a lack of barriers or a high level of ZIKV-related knowledge.


Asunto(s)
Tamizaje Masivo/psicología , Percepción , Mujeres Embarazadas/psicología , Ajuste de Riesgo/normas , Infección por el Virus Zika/diagnóstico , Adolescente , Adulto , Femenino , Florida , Humanos , Tamizaje Masivo/métodos , Tamizaje Masivo/estadística & datos numéricos , Embarazo , Ajuste de Riesgo/métodos , Medición de Riesgo/métodos , Encuestas y Cuestionarios , Virus Zika/patogenicidad , Infección por el Virus Zika/psicología
15.
Int J Mol Sci ; 20(12)2019 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-31234341

RESUMEN

Zika virus (ZIKV) transmission can cause serious fetal neurological abnormalities. ZIKV persistence in various human cells and tissues can serve as infectious reservoirs and post serious threats to public health. The human embryonic kidney (HEK293) cell line with known neuronal developmental properties was readily infected by ZIKV in a strain-dependent fashion. Significant cytopathic effect in HEK293 cells infected by the prototype MR 766 strain of ZIKV resulted in complete loss of cells, while small numbers of HEK293 cells infected by contemporary ZIKV isolates (PRV or FLR strain) continued to survive and regrow to confluency in the culture around two months after initial infection. Most, if not all, of the cells in the two resulting persistently ZIKV-infected HEK293 cell lines tested positive for ZIKV antigen. Compared to HEK293 control cells, the persistently ZIKV-infected HEK293 cells had slower growth rates with some cells undergoing apoptosis in culture. The "persistent ZIKVs" produced constitutively by both PRV and FLR strains ZIKV-infected HEK293 cells had significantly attenuated cell infectivity and/or cytopathogenicity. Comparative genome sequence analyses between the persistent ZIKVs and the original inoculum ZIKVs showed no clonal selection with specific gene mutations in the prolonged process of establishing persistently PRV strain ZIKV-infected HEK293 cells; while selection of ZIKV subclones with mutations in the envelope, protein pr and multiple NS genes was evident in developing persistently FLR strain ZIKV-infected HEK293 cell line. Our study provides molecular insights into the complex interplays of ZIKV and human host cells in establishing ZIKV persistence.


Asunto(s)
Infección por el Virus Zika/patología , Virus Zika/fisiología , Animales , Apoptosis , Efecto Citopatogénico Viral , Genoma Viral , Genómica , Células HEK293 , Humanos , Mutación , Células Vero , Virus Zika/genética , Virus Zika/patogenicidad , Infección por el Virus Zika/virología
16.
Bioessays ; 41(6): e1800239, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-31106880

RESUMEN

Previous studies of Zika virus (ZIKV) pathogenesis have focused primarily on virus-driven pathology and neurotoxicity, as well as host-related changes in cell proliferation, autophagy, immunity, and uterine function. It is now hypothesized that ZIKV pathogenesis arises instead as an (unintended) consequence of host innate immunity, specifically, as the side effect of an otherwise well-functioning machine. The hypothesis presented here suggests a new way of thinking about the role of host immune mechanisms in disease pathogenesis, focusing on dysregulation of post-transcriptional RNA editing as a candidate driver of a broad range of observed neurodevelopmental defects and neurodegenerative clinical symptoms in both infants and adults linked with ZIKV infections. The authors collect and synthesize existing evidence of ZIKV-mediated changes in the expression of adenosine deaminases acting on RNA (ADARs), known links between abnormal RNA editing and pathogenesis, as well as ideas for future research directions, including potential treatment strategies.


Asunto(s)
Síndrome de Guillain-Barré/patología , Síndrome de Guillain-Barré/virología , Edición de ARN , Infección por el Virus Zika/patología , Infección por el Virus Zika/virología , Virus Zika/patogenicidad , Adenosina Desaminasa/genética , Adulto , Biomarcadores , Femenino , Expresión Génica , Interacciones Huésped-Patógeno/inmunología , Humanos , Inmunidad Innata , Lactante , Recién Nacido , Microcefalia/virología , Embarazo , Proteínas de Unión al ARN/genética
17.
mSphere ; 4(3)2019 05 08.
Artículo en Inglés | MEDLINE | ID: mdl-31068433

RESUMEN

Zika virus (ZIKV) infection during pregnancy leads to devastating fetal outcomes, including neurological (microcephaly) and ocular pathologies such as retinal lesions, optic nerve abnormalities, chorioretinal atrophy, and congenital glaucoma. Only clinical case reports have linked ZIKV infection to causing glaucoma, a major blinding eye disease. In the present study, we have investigated the role of ZIKV in glaucoma pathophysiology using in vitro and in vivo experimental models. We showed that human primary trabecular meshwork (Pr. TM) cells, as well as a human GTM3 cell line, were permissive to ZIKV infection. ZIKV induced the transcription of various genes expressing pattern recognition receptors (TLR2, TLR3, and RIG-I), cytokines/chemokines (TNF-α, IL-1ß, CCL5, and CXCL10), interferons (IFN-α2, IFN-ß1, and IFN-γ), and interferon-stimulated genes (ISG15 and OAS2) in Pr. TM cells. ZIKV infection in IFNAR1-/- and wild-type (WT) mouse eyes resulted in increased intraocular pressure (IOP) and the development of chorioretinal atrophy. Anterior chamber (AC) inoculation of ZIKV caused infectivity in iridocorneal angle and TM, leading to the death of TM cells in the mouse eyes. Moreover, anterior segment tissue of infected eyes exhibited increased expression of inflammatory mediators and interferons. Furthermore, ZIKV infection in IFNAR1-/- mice resulted in retinal ganglion cell (RGC) death and loss, coinciding with optic nerve infectivity and disruption of anterograde axonal transport. Because of similarity in glaucomatous pathologies in our study and other experimental glaucoma models, ZIKV infection can be used to study infectious triggers of glaucoma, currently an understudied area of investigation.IMPORTANCE Ocular complications due to ZIKV infection remains a major public health concern because of their ability to cause visual impairment or blindness. Most of the previous studies have shown ZIKV-induced ocular pathology in the posterior segment (i.e., retina) of the eye. However, some recent clinical reports from affected countries highlighted the importance of ZIKV in affecting the anterior segment of the eye and causing congenital glaucoma. Because glaucoma is the second leading cause of blindness worldwide, it is imperative to study ZIKV infection in causing glaucoma to identify potential targets for therapeutic intervention. In this study, we discovered that ZIKV permissively infects human TM cells and evokes inflammatory responses causing trabeculitis. Using a mouse model, we demonstrated that ZIKV infection resulted in higher IOP, increased RGC loss, and optic nerve abnormalities, the classical hallmarks of glaucoma. Collectively, our study provides new insights into ocular ZIKV infection resulting in glaucomatous pathology.


Asunto(s)
Ojo/patología , Ojo/virología , Glaucoma/virología , Malla Trabecular/virología , Infección por el Virus Zika/complicaciones , Virus Zika/patogenicidad , Animales , Muerte Celular , Línea Celular , Quimiocinas/genética , Citocinas/genética , Modelos Animales de Enfermedad , Femenino , Glaucoma/fisiopatología , Humanos , Interferones/genética , Presión Intraocular , Masculino , Ratones , Ratones Endogámicos C57BL , Células Ganglionares de la Retina/patología , Células Ganglionares de la Retina/virología , Malla Trabecular/patología , Transcriptoma , Infección por el Virus Zika/patología
18.
Gene ; 708: 57-62, 2019 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-31128224

RESUMEN

Zika virus (ZIKV) has spread globally and has been linked to the onset of microcephaly and other brain abnormalities. The ZIKV genome consists of an ~10.7 kb positive-stranded RNA molecule that encodes three structural (C, prM and E) and seven nonstructural (5'-NS1-NS2A-NS2B-NS3- NS4A/2K-NS4B-NS5-3') proteins. In this work, we looked for genetic variants in 485 ZIKV complete genomes from GenBank (NCBI) and performed a computational systematic approach using MAESTROweb server to assess the impact of nonsynonymous mutations in ZIKV proteins (C, M, E, NS1, NS2A, NS2B-NS3 protease, NS3 helicase and NS5). Then, we merged the data and correlated it with the phenotypic reports of ZIKV circulating strains. The sensitivity profile of the proteins showed 96 mutational hotspots. We found 22 relevant mutations in proteins C (I80T), NS2A (I34M/T/V, I45V, I80T/V, L113F, A117V, I118V, L128P, V143A, T151A, M199I/V, R207K and L208I) and NS3 helicase (D436G, Y498H, R525K, Q528R and R583K) of the circulating strains. Our analysis exploited the impact of nonsynonymous mutations on ZIKV proteins, their structural and functional insights. The results presented here could advance our current understanding on ZIKV proteins functions and pathogenesis.


Asunto(s)
Genoma Viral/genética , Proteínas Virales/genética , Virus Zika/genética , Secuencia de Aminoácidos/genética , Mutación , Conformación Proteica , Virus Zika/patogenicidad
19.
J Med Internet Res ; 21(5): e13090, 2019 05 13.
Artículo en Inglés | MEDLINE | ID: mdl-31094347

RESUMEN

BACKGROUND: An estimated 3.9 billion individuals live in a location endemic for common mosquito-borne diseases. The emergence of Zika virus in South America in 2015 marked the largest known Zika outbreak and caused hundreds of thousands of infections. Internet data have shown promise in identifying human behaviors relevant for tracking and understanding other diseases. OBJECTIVE: Using Twitter posts regarding the 2015-16 Zika virus outbreak, we sought to identify and describe considerations and self-disclosures of a specific behavior change relevant to the spread of disease-travel cancellation. If this type of behavior is identifiable in Twitter, this approach may provide an additional source of data for disease modeling. METHODS: We combined keyword filtering and machine learning classification to identify first-person reactions to Zika in 29,386 English-language tweets in the context of travel, including considerations and reports of travel cancellation. We further explored demographic, network, and linguistic characteristics of users who change their behavior compared with control groups. RESULTS: We found differences in the demographics, social networks, and linguistic patterns of 1567 individuals identified as changing or considering changing travel behavior in response to Zika as compared with a control sample of Twitter users. We found significant differences between geographic areas in the United States, significantly more discussion by women than men, and some evidence of differences in levels of exposure to Zika-related information. CONCLUSIONS: Our findings have implications for informing the ways in which public health organizations communicate with the public on social media, and the findings contribute to our understanding of the ways in which the public perceives and acts on risks of emerging infectious diseases.


Asunto(s)
Brotes de Enfermedades/estadística & datos numéricos , Conductas Relacionadas con la Salud , Salud Pública/tendencias , Medios de Comunicación Sociales/tendencias , Infección por el Virus Zika/epidemiología , Virus Zika/patogenicidad , Femenino , Humanos , Masculino , Estados Unidos
20.
PLoS Pathog ; 15(4): e1007640, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30998804

RESUMEN

Zika virus (ZIKV) has been known for decades to circulate in Africa and Asia. However, major complications of a ZIKV infection have recently become apparent for reasons that are still not fully elucidated. One of the hypotheses for the seemingly increased pathogenicity of ZIKV is that cross-reactive dengue antibodies can enhance a ZIKV infection through the principle of antibody-dependent enhancement (ADE). Recently, ADE in ZIKV infection has been studied, but conclusive evidence for the clinical importance of this principle in a ZIKV infection is lacking. Conversely, the widespread circulation of ZIKV in dengue virus (DENV)-endemic regions raises new questions about the potential contribution of ZIKV antibodies to DENV ADE. In this review, we summarize the results of the evidence to date and elaborate on other possible detrimental effects of cross-reactive flavivirus antibodies, both for ZIKV infection and the risk of ZIKV-related congenital anomalies, DENV infection, and dengue hemorrhagic fever.


Asunto(s)
Anticuerpos Antivirales/inmunología , Acrecentamiento Dependiente de Anticuerpo/inmunología , Reacciones Cruzadas/inmunología , Efecto Citopatogénico Viral/inmunología , Dengue/inmunología , Infección por el Virus Zika/inmunología , Virus Zika/patogenicidad , Dengue/virología , Humanos , Infección por el Virus Zika/prevención & control , Infección por el Virus Zika/virología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA