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Multiloop splitter-based non-cryogenic artificial trapping (M-SNAT) modulation technique was developed, miniaturized and applied in comprehensive two-dimensional gas chromatography (GC×GC) for analysis of cannabis samples. The approach employed deactivated fuse silica (DFS) columns configured into multiple loop splitter system halving the perimeters of the progressively upstream loops. This splitter device was located between the first (1D) semi-nonpolar column outlet and a microfluidic Deans switch (DS). Each splitter loop splits a peak into two subpeaks having the same area with different void times. Three loops were then applied resulting in the number of the split subpeaks (nsplit) of 8 for each peak, and retention time differences between any two adjacent subpeaks (∆tR,split) were the same. By applying periodic heartcut event (H/C) within every artificial modulation period (PAM) of nsplit×∆tR,split, comprehensive split-and-trapped modulation profiles of analytes could be selectively transferred onto the second (2D) polar column (30â¯m) without cryogen consumption. This artificial modulation system was applied for analysis of cannabis samples with enhanced 2D peak capacity (2ncâ¼15). The established method was applied to analyse cannabis extracts using vegetable oils with or without frying process. This reveals 454 different peaks with 76, 92, 35 and 70 specific components specifically observed by using olive oil extraction (OE), fried OE, coconut oil extraction (CE) and fried CE, respectively.
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Cannabis , Cannabis/química , Cromatografía de Gases/métodos , Cromatografía de Gases/instrumentación , MiniaturizaciónRESUMEN
BACKGROUND: Diagnosis of stress generally involves uses of questionnaires which can provide biased results. The more reliable approach relies on observation of individual symptoms by psychiatrists which is time consuming and could not be applicable for massive scale screening tests. This research established alternative approaches with gas chromatography-ion mobility spectrometry (GC-IMS) and electronic nose (e-nose) to perform fast stress screening based on fingerprinting of highly volatile compounds in headspaces of sweat. The investigated samples were obtained from 154 female nurse volunteers who also provided the data of questionnaire-based mental health scores with the high stress cases confirmed by psychiatrists. RESULTS: The interviews by psychiatrists revealed 14 volunteers with high stress. Their axillary sweat samples and that from 32 nurses with low/moderate stress (controls) were collected onto cotton rods and analysed with GC-IMS. The possible marker peaks were selected based on the accuracy data. They were tentatively identified as ammonia, diethyl ether, methanol, octane, pentane, acetone and dimethylamine which could involve different endogenous mechanisms or the relationships with the local microbiomes. The data were further analysed using partial least squares discriminant analysis with the receiver operating characteristic curves showing the optimum accuracy, sensitivity and selectivity of 87%, 86% and 88%, respectively. Providing that the samples were obtained from the nurses without deodorant uses, the high stress cases could be screened using e-nose sensors with the accuracy of 89%. The sensor responses could be correlated with the marker peak area data in GC-IMS with the coefficients ranging from -0.70 to 0.80. SIGNIFICANCE: This represents the first investigation of highly volatile compound markers in sweat for high stress screening. The established methods were simple, reliable, rapid and non-invasive, which could be further adapted into the portable platform of e-nose sensors with the practical application to perform the screening tests for nurses in Phra Nakorn Si Ayutthaya hospital, Thailand.
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Nariz Electrónica , Espectrometría de Movilidad Iónica , Sudor , Humanos , Sudor/química , Femenino , Espectrometría de Movilidad Iónica/métodos , Adulto , Cromatografía de Gases y Espectrometría de Masas , Estrés Psicológico/diagnóstico , Compuestos Orgánicos Volátiles/análisis , Persona de Mediana EdadRESUMEN
This study established a novel infield sensing approach based on detection of the volatile compound markers in skin secretions. This was based on analysis of volatile compounds in axillary sweat samples collected from RT-PCR-proven Coronavirus disease 2019 (COVID-19) positive and negative populations using gas chromatography-mass spectrometry (GC-MS). The analysis proposed the possible markers of the monoaromatic compounds and ethyl hexyl acrylate. A portable photo ionization detector (PID) incorporated with the selective material towards the marker compounds was then developed with the pressurized injection approach. This provided the accuracy of 100% in the research phase (n = 125). The developed approach was then applied for screening of 2207 COVID-19 related cases covering the periods of the Alpha, Beta, Delta and Omicron variants of SARS-CoV-2 infection in Bangkok, Thailand. This offered the sensitivity, specificity and accuracy ranges of 92-99, 93-98 and 95-97%, respectively.
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COVID-19 , Cromatografía de Gases y Espectrometría de Masas , SARS-CoV-2 , Sudor , Compuestos Orgánicos Volátiles , Humanos , COVID-19/diagnóstico , COVID-19/virología , Sudor/química , Compuestos Orgánicos Volátiles/análisis , SARS-CoV-2/aislamiento & purificación , Cromatografía de Gases y Espectrometría de Masas/métodos , Femenino , Masculino , Adulto , Persona de Mediana Edad , Sensibilidad y Especificidad , Tailandia , Prueba de COVID-19/métodos , Biomarcadores/análisis , Anciano , Adulto JovenRESUMEN
BACKGROUND: To investigate the effect of a 50% ascorbic acid with 50% citric acid solution on the immediate shear bond strength (SBS) of metallic brackets after tooth bleaching. The enamel etching pattern and the required quantity of these combined acids as antioxidants following 35% hydrogen peroxide (HP) bleaching were also determined. METHODS: The stability of the solution at room temperature was assessed at various time intervals. Fifty teeth were randomly divided into five groups: non-bleached (G1), bleached then acid etched (G2), bleached followed by a 10-minute treatment with 10% sodium ascorbate and acid etched (G3), 5-minute treatment with 50% ascorbic acid (G4), and 5-minute treatment with a combination of 50% ascorbic acid and 50% citric acid (G5). Groups G2, G3, G4 and G5 were bleached by 35% HP gel for a total of 32 min. Acid etching in groups G1, G2, and G3 was performed using 37% phosphoric acid (Ormco®, Orange, CA, USA) for 15 s. In all groups, metal brackets were immediately bonded using Transbond™ XT primer and Transbond™ PLUS adhesive, with light curing for 40 s. The SBS was tested with a universal testing machine, and statistical analysis was conducted using one-way ANOVA followed by Tukey's HSD test. The level of significance was set at p < 0.05 for all statistical tests. RESULTS: Stability tests demonstrated that the combined acids remained effective for up to 21 days. Group G5 significantly increased the SBS of bleached teeth to the level of G1 (p < 0.05), while G3 did not achieve the same increase in SBS (p > 0.05). SEM analysis revealed enamel etching patterns similar to those of both control groups (G1 and G2). Kinetic studies at 6 min indicated that the antioxidation in G5 reacted 0.2 mmole lower than in G3 and G4. CONCLUSION: 5-minute application of the combined acids enhanced the SBS of bleached teeth comparable to unbleached teeth. The combined acids remain stable over two weeks, presenting a time-efficient, single-step solution for antioxidant application and enamel etching in orthodontic bracket bonding.
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Ácido Ascórbico , Ácido Cítrico , Recubrimiento Dental Adhesivo , Esmalte Dental , Soportes Ortodóncicos , Resistencia al Corte , Blanqueamiento de Dientes , Ácido Ascórbico/farmacología , Ácido Cítrico/farmacología , Ácido Cítrico/química , Blanqueamiento de Dientes/métodos , Humanos , Proyectos Piloto , Esmalte Dental/efectos de los fármacos , Recubrimiento Dental Adhesivo/métodos , Grabado Ácido Dental , Antioxidantes/farmacología , Propiedades de Superficie , Factores de Tiempo , Peróxido de Hidrógeno/química , Blanqueadores Dentales/química , Ácidos Fosfóricos , Análisis del Estrés DentalRESUMEN
This study presents a novel tandem mass spectrometry (MS/MS) approach utilizing a data independent acquisition (DIA) concept specifically designed with gas chromatography-electron ionization-triple quadrupole mass spectrometry (GC-EI-QqQMS). This allows compound identification based on comparison between all the experimental MS/MS product ion spectra and the simulated library data of >1,000 MS/MS transitions of 71 compounds. The simulation data were generated by using the Competitive Fragmentation Modeling (CFM-ID) 3.0 program. The approach for calculation of the DIA MS/MS library match scores was then established and applied for identification of a range of terpenoids and oxygenated compounds in perfume. The identity of each peak was confirmed using 4-241 MS/MS transitions. The established data collection and analysis methods are expected to be useful for increased confidence in volatile compound analysis.
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Espectrometría de Masas en Tándem , Espectrometría de Masas en Tándem/métodos , Cromatografía de Gases y Espectrometría de Masas/métodosRESUMEN
Simple approach for rapid screening of corona virus disease 2019 (COVID-19) has been developed. This applied gas chromatography-flame ionization detector (GC-FID) analyzing the potential compound marker in sweat samples obtained from COVID-19 positive and negative volunteers in Bangkok, Thailand. The samples were collected by using cotton rods for 15 min, heated at 90 °C for 5 min, and the volatile compounds in the headspace (HS) were injected (5.00 mL) at 150 °C and separated within 13.7 min. The marker peak was tentatively identified as p-cymene by the authentic standard injection and comparison with the GC-mass spectrometry (GC-MS) and comprehensive two-dimensional GC (GC × GC)-MS analysis. Possible mechanisms for the presence of p-cymene were proposed. The marker peak area thresholds were then varied and optimized via construction of the receiver operating characteristic (ROC) curve. With the optimum threshold, the established method offered the accuracy, sensitivity and specificity of 96 %. This method was insignificantly affected (p-value >0.05) by genders, body mass indices, ages, and use of deodorants as well as the p-cymene containing food. However, the performance could be affected by the population with personal hygiene or experiencing the microbiomes producing p-cymene.
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COVID-19 , Sudor , Masculino , Femenino , Humanos , Ionización de Llama/métodos , Cromatografía de Gases y Espectrometría de Masas/métodos , COVID-19/diagnóstico , TailandiaRESUMEN
During the corona virus disease 2019 (COVID-19) pandemic period, rapid screening of covid-19 patients has been of great interest by developing a fluorescent sensor for complexation with nonanal, which is a marker for Covid-19 detection in sweat. Solid phase micro-extraction gas chromatography-mass spectrometry (SPME GC-MS) was initially used to quantify nonanal in armpit sweat samples based on an external calibration curve. A sample containing a nonanal content above the threshold of 1.04 µL is expected to be COVID-19 positive with a sensitivity and specificity of 87% and 89%, respectively, validated by comparison with RT-PCR results. For more practical applications, helicene dye-encapsulated ethyl cellulose, namely EC@dyeNH, was applied to screen 140 sweat samples collected from the foreheads of volunteers. The mixed sensor and sweat solution droplets were then visualized and imaged under blacklight. The COVID-19 positive droplets exhibited yellow fluorescence emission, the brightness of which could be measured by using ImageJ in the grey scale. With the optimum color intensity of >73 for positive results, the screening performance was observed with a sensitivity and specificity of 96% and 93%, respectively. The overall test time of this method is approximately less than 15 min. This alternative method offers a promising practical screening approach for the diagnosis of COVID-19 in sweat.
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COVID-19 , Humanos , Cromatografía de Gases y Espectrometría de Masas , COVID-19/diagnóstico , Sudor/química , Sudor/virología , Prueba de COVID-19RESUMEN
A multiloop splitter-based non-cryogenic artificial trapping (M-SNAT) modulation technique was established, which applied the first (1D) nonpolar and the second (2D) polar columns, deactivated fused silica (DFS) columns, a microfluidic Deans switch (DS), and splitters located between the 1D column outlet and the DS. The splitters were connected into multiple loops with a progressively doubled perimeter of the next loop. This enabled a duplex splitting mechanism within each loop consisting of splitting of analyte pulses, the pulse delay, and their combination which led to equally split peaks of the same analytes with the number of split peaks (nsplit) equal to 2m (m = number of loops). This system resulted in local profiles of artificially split-and-trapped analytes prior to their selective transfers onto the 2D column by means of periodic multiple heart-cuts (H/C). The developed SNAT approach can be successful, providing that the ratio of modulation period to sampling time (PM/tsamp) is equal to nsplit. The approach with nsplit = 16 was further developed into a single device platform and applied for the modulation of a wide range of compounds in waste tire pyrolysis samples with the RSD of ≤0.01 and <10% for the one-dimensional modulated peak times and peak areas, respectively (n = 50). The method enabled an artificial modulation mechanism without cryogen consumption and enhanced the 2D peak capacity (2nc) and 2D separation by use of a longer 2D column.
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Over the past years, lung cancer has been one of the vital cancer-related mortalities worldwide and has inevitably exhibited the highest death rate with the subsequent need for facile and convenient diagnosis approaches to identify the severity of cancer. Previous research has reported long-chain aldehyde compounds such as hexanal, heptanal, octanal, and nonanal as potential biomarkers of lung cancer. Herein, the helicene dye-encapsulated ethyl cellulose (EC@dye-NH) nanosensors have been applied for the potentially sensitive and specific detection of long-chain aldehydes in aqueous media. The sensors contain the intrinsic hydrazide group of dye-NH, which is capable of reacting an aldehyde group via imine formation and the EC backbone. This offers the synergistic forces of hydrophobic interactions with alkyl long-chain aldehydes, which could induce self-assembly encapsulation of EC@dye-NH nanosensors and strong fluorescence responses. The addition of long-chain aldehyde would induce the complete micellar-like nanoparticle formation within 15 min in acetate buffer pH 5.0. The limit of detection (LOD) values of EC@dye-NH nanosensors toward heptanal, octanal, and nonanal were 40, 100, and 10 µM, respectively, without interference from the lung fluid matrices and short-chain aldehydes. For practical applicability, this sensing platform was developed for quantification of the long-chain aldehydes in lung fluid samples with 98-101% recoveries. This EC@dye-NH nanosensor was applied to quantify nonanal contents in lung fluid samples. The results of this method based on EC@dye-NH nanosensors were then validated using standard gas chromatography-mass spectrometry (GC-MS), which gave results consistent with the proposed method. With intracellular imaging application, the EC@dye-NH nanosensors demonstrated excellent intracellular uptake and strong green fluorescence emission upon introducing the nonanal into the lung cancer cells (A549). Thus, the developed nanosensing approach served as the potential fluorescent probes in medical and biological fields, especially for lung cancer disease diagnosis based on highly selective and sensitive detection of long-chain aldehydes.
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This study developed a novel voltage step electrocoagulation (VSEC) technique on paper for analysis and classification of red wine samples. The concept relies on the electrode system applying voltage steps along a strip of filter paper soaked with a wine sample. The system employed a cathode array system (CAS) for voltage step application and an aluminium anode undergoing corrosion to form the green sludges responsible for wine color bleaching along the paper. The VSEC technique led to the shade of colors along the paper which can be observed by the naked-eye or using image processing software. The system was applied to classify 15 wine samples into different groups and to perform dilution and adulteration tests. In addition, the approach could be applied to approximate antioxidant properties of the wine samples as observed via the correlation between the results from VSEC and IC50 values obtained from the DPPH assay with an R2 of 0.76.
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Vino , Vino/análisis , Antioxidantes/análisis , Ácido Hipocloroso/análisis , ElectrocoagulaciónRESUMEN
In this work, first and second dimensional retention index (1I and 2I) based calculation approach is established to simulate peak retention times (1tR and 2tR) of samples for the given sets of volatile compounds in comprehensive two-dimensional gas chromatography-mass spectrometry (GC×GC-MS). For the result without 1tR and 2tR data of alkane references (1tR(n) and 2tR(n)), the following steps were applied: (1) curve fitting based on van den Dool and Kratz relationship in order to simulate 1tR(n) using a training set of volatile compounds in a sample with their experimental 1tR data, and (2) simulation of 2tR(n) at different 1tR(n) to construct their isovolatility curves based on a nonlinear equation with p1-p5 parameters and a constant (within the ranges of -0.0052 to 0.0049, -0.6181 to -0.0230, -26.4775 to -0.2698, 0.0050 to 9.6259, -7.2976 to -3.9524 and 0.9157 to 4.0779, respectively). These parameters were obtained by performing curve fitting according to the experimental 2tR data of the same training set with the least square values ranging from 4.58×10-15 to 32.55. Simulation of 1tR and 2tR of target analytes (1tR,sim and 2tR,sim) with known 1I and 2I were performed using 1tR(n) and the simulated isovolatility curves. All the calculations and curve fittings were carried out by using Solver in Microsoft Excel. The approach was applied to simulate results for 542 compounds in several samples including analysis of saffron (Crocus sativas L.), Boswellia papyrifera, acacia honey and incense powder/smoke, perfume and cannabis either reported from literature or from the experiments in this work using different experimental approaches. These were compared with the experimental data showing correlation with the R2 ranges of 0.98-1.00 and 0.80-0.97 for 1tR and 2tR, respectively. This approach was then applied to propose 6 compounds which may be incorrectly identified based on the differences of >2 times of the standard deviations between 2tR,sim and the experimental 2tR in both residue and leave-one-out analyses.
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Alcanos , Crocus , Simulación por Computador , Cromatografía de Gases y Espectrometría de Masas , Análisis de los Mínimos Cuadrados , HumoRESUMEN
High-temperature comprehensive two-dimensional gas chromatography (HTGC × GC) using a longitudinally modulated cryogenic system (LMCS) was developed for the analysis of low-volatility pesticides in cabbage. The method applied DB-17HT and DB-5HT as the first and second dimensional (1D and 2D) columns, respectively. Twelve pesticides, namely 6 organochlorines (4,4'-DDT, ß-endosulfan, endosulfan sulfate, endrin, heptachlor, and dicofol), 4 carbamates (metolcarb, isoprocarb, methiocarb, and carbofuran), 1 organophosphate (chlorpyrifos), and 1 pyrethroid (permethrin), were spiked into cabbage samples and prepared using QuEChERS. The applied oven temperature was up to 340 °C, enabling the elution of all the target pesticides and the matrix. The effects of initial oven temperature program, temperature ramp rate, LMCS trap temperature, and modulation period (PM) on the separation results were investigated, leading to the suitable conditions of 80 °C, 15 °C min-1, 10 °C, and 12 s, respectively. The method detection limits, signal-to-noise ratio, and recoveries of the compounds were within the ranges of 0.01-0.09 mg kg-1, 4.26-32.7, and 78-104%, respectively. Good linearity ranges within the concentration range of 0.1-1 ppm with R2 > 0.9134 were also obtained with the intra and interday precisions of the peak areas of 0.4-9.8% and 1.0-10.2%, respectively.
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Brassica , Hidrocarburos Clorados , Plaguicidas , Cromatografía de Gases/métodos , Hidrocarburos Clorados/análisis , Plaguicidas/análisis , TemperaturaRESUMEN
An improved strategy for compound identification incorporating gas chromatography hyphenated with Fourier transform infrared spectroscopy and mass spectroscopy (GC-FTIR/MS) is reported. (Over)reliance on MS may lead either to ambiguous identity or to incorrect identification of a compound. However, the MS result is useful to provide a cohort of possible compounds. The IR result for each tentative compound match was then simulated using molecular modeling, to provide functional group and isomer differentiation information, and then compared with the experimental FTIR result, offering identification based on both MS and IR. Several basis sets were evaluated for IR simulations; Def2-TZVPP was a suitable basis set and correlated well with experimental data. The approach was applied to industrial applications, confirming the isomers of 2,3-bis(thiosulfanyl)-but-2-enedinitrile, bromination products of 1-bromo-2,3-dimethylbenzene, and autoxidative degradation of phenyl-di-tert-butylphosphine.
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Cromatografía de Gases y Espectrometría de Masas , Humanos , Isomerismo , Espectrometría de Masas , Espectrofotometría Infrarroja , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Experimental and data analysis approaches in multidimensional gas chromatography (MDGC) comprising comprehensive multiple heart-cut (H/C) and comprehensive two dimensional GC (GC × GC) were developed with an example application illustrated for analysis of a technical glycol precursor sample. The GC × GC system employed a long 1D (30 m) and a short 2D (5 m) column with a flow modulator and a Deans switch (DS) as a splitter; meanwhile. The H/C system was applied solely as a DS located between long 1D (30 m) and 2D (60 m) columns without use of cryogenic trapping devices. The effects of injection time and 2D column flow in GC × GC and the impacts of H/C window and number of injections (total analysis time) in H/C analysis were investigated. The analysis performance for each condition was evaluated according to peak capacity and number of separated compounds. The continuum between the two techniques was then established via the relationship between analysis time and analysis performance. The separation performances were improved with longer analysis time so that the suitable condition was selected within this compromise. Under the selected conditions, volatile compounds in the technical glycol precursor sample were identified according to the match between the experimental MS spectra and first dimensional retention indices (1 I) with that from the NIST2014 database and literature. An hour analysis with GC × GC resulted in a total peak capacity of 798, number of separated peaks of 61 and average MS match score of 887 ± 35; meanwhile, the corresponding numbers were improved to be 9198, 107 and 898 ± 24, respectively, with the 25 h comprehensive H/C analysis.
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Electrocoagulation (EC) approach was developed to allow fast sample cleanup step prior to selective analysis of non- and mono-hydroxylated phenolic acids in red wine samples with high performance liquid chromatography hyphenated with UV detection (HPLC-UV). EC system with the wine in KCl(aq) electrolyte (1.5 mol L-1) was employed removing the polymeric pigments with good recovery of 39 peaks from 64 peaks. The mechanisms mainly involve enrichment induced aggregation and reduction of the pigments at the cathode and the adsorption onto the EC sludge. The EC was further miniaturized employing two intercalated stainless steel spring electrodes at 9.0 V which allowed removal of >99% interference peak area from the pigments within 5 s. The recoveries of the target phenolic acids (p-hydroxybenzoic acid, vanillic acid, syringic acid and ferulic acid) were within the range of 86-102%. The repeated analysis of these standards revealed <2 and ≤10% RSD of the intra-day and inter-day precisions, respectively. The linearities of their calibration curves were observed with R 2 > 0.99. Their method detection limits were within the range of 0.02-0.20 mg L-1.
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Comprehensive heart-cut multidimensional gas chromatography (CH/C MDGC) without a cryogenic trapping device was developed with an established approach for calculation of first and second dimensional retention indices (1I and 2I) for improved compound identification. A first dimensional (1D) DB-1MS column (60 m) and a second dimensional (2D) DB-WAX column (60 m) were applied with a Deans switch (DS) using a constant H/C window of 0.2 min and a periodic multiple heartcut strategy comprising 225H/C throughout the CH/C. 1I was calculated based on comparison of the middle of the heartcut time with the alkane retention times on the 1D column. A multi-location peak parking approach using sixteen sets of automated injections of alkane references was also established with the least square curve fitting method for construction of the alkane isovolatility curves which were applied for 2I calculation. The untargeted compound analysis of a perfume sample was then performed according to comparison with the libraries of mass spectra, 1I and 2I. The CH/C MDGC system with a 25 h analysis time showed a peak capacity (nc) of 9198 and 128 separated peaks with 71 compounds successfully identified according to MS, 1I and 2I library match under the established error approximation criteria. Furthermore, relationship between the analysis time and number of separated peaks was proposed based on the set of 84 identifiable compounds. With the compensation of lower separation performance and greater I errors, the analysis time could be reduced by applying a 2.5 min H/C window with a total analysis time of 2 h and nc of 1134.
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In this study, thin-layer chromatography was applied for selective extraction of volatile compounds in perfume prior to analysis with solid phase microextraction and gas chromatography-mass spectrometry. The standard compounds were desorbed from the thin-layer chromatography plate and extracted at 80°C for 15 min showing good linearity of the calibration curves (R2 > 0.98) and acceptable recovery range (65-81%). The plate after the separation was cut into four smaller parts followed by solid phase microextraction/gas chromatography-mass spectrometry analysis, which revealed different compound profile in each part with the correlation between log P of the standard compounds and their positions along the thin-layer chromatography plate (R2 = 0.65). This approach was applied to analyze perfume compounds in the sample with strong matrix interference from the synthetic agarwood. Terpene hydrocarbons (woody-based odors), ketones/esters, aldehydes, ethers, and alcohols were mostly observed at 8 ± 1, 6 ± 1, 5 ± 2, 4 ± 2, and 3 ± 2 cm, respectively, from the bottom of the thin-layer chromatography plate. While, the conventional solid phase microextraction/gas chromatography-mass spectrometry analysis of this sample solution revealed only 62 compounds (including 35 perfume compounds), the four-piece approach resulted in 109 compounds (62 perfume compounds). Furthermore, the capability of thin-layer chromatography-Gas Analyzer approach to analyze the isomers in this complex sample was demonstrated.
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Comprehensive heartcut multidimensional gas chromatography was applied with example application for analysis of a sample obtained from palmitic acid oxidation in a Rancimat instrument. The system utilized a single Deans switch (DS) located between first dimensional semi-standard nonpolar (30 m) and second dimensional polar (60 m) columns. A cyclic multiple heartcut strategy consisting of 150 heartcuts with a 0.2 min window was applied offering comprehensive analysis and injection of a narrow band of compounds onto the second column without use of cryogenic trapping devices. Untargeted compound analysis of the sample prepared by solid phase micro-extraction was performed based on match between the experimental MS spectra and first dimensional retention indices with that from the NIST library. The sample contained the major compounds of 2-octanone, 1-methylcyclohexanol, 2,3,6-trimethylphenol, 3-phenylpropanol and 2-nonanone. This approach was then evaluated based on peak capacity and the number of identified compounds. Compared with one dimensional gas chromatography providing a total peak capacity of 172 and 43 identified compounds, the analysis performance was much more improved with a capacity of 5840 and 235 identified compounds by using comprehensive heartcut multidimensional gas chromatography with the total analysis time of 15.3 h. By comparison within the same set of identified compounds, the one dimensional and multidimensional approaches provided the MS match scores of 769 ± 81 and 836 ± 88, respectively. In addition, the nonlinear relationship between the analysis time and number of identifiable peaks was calculated according to the set of 235 compounds. This revealed that the analysis time could be shortened with the compensation of lower separation performance, where application of a 2.5 min heartcut window with the total analysis time of 1.2 h could result in the total peak capacity of 390 with 150 identifiable compounds.
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Chiral cyclic trilactams ((+)-1 and (-)-1) with C3 symmetry were investigated as liquid phase extraction materials of volatile compounds. Perfume samples, involving a range of chiral odor active terpenoids, were applied and each sample before and after the liquid phase extraction was analyzed by solid phase microextraction (SPME)-gas chromatography hyphenated with mass spectrometry. It was found that (+)-1 exhibited significantly higher enrichment factors for several terpenoids, while (-)-1 did not. The mode of interactions between each enantiomer and l-menthol and Kharismal (methyl dihydrojasmonate) was further investigated by molecular dynamics (MD) simulations and theoretical density functional theory (DFT) calculations, showing the favorable interactions of enriched substrates with (+)-1 through noncovalent interactions, either hydrogen bonds or electrostatic interactions.