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1.
Foods ; 13(12)2024 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-38928737

RESUMEN

Hydroalcoholic extracts from Malbec and Torrontés wine pomaces (Vitis vinifera L.) originating from the high-altitude vineyards of Argentina's Calchaquí Valleys were characterized. Total phenolics, hydroxycinnamic acids, orthodiphenols, anthocyanins, non-flavonoid phenolics, total flavonoids, flavones/flavonols, flavanones/dihydroflavonols, and tannins were quantified through spectrophotometric methods, with the Malbec extract exhibiting higher concentrations in most of phytochemical groups when compared to Torrontés. HPLC-DAD identified more than 30 phenolic compounds in both extracts. Malbec displayed superior antiradical activity (ABTS cation, nitric oxide, and superoxide anion radicals), reduction power (iron, copper, and phosphomolybdenum), hypochlorite scavenging, and iron chelating ability compared to Torrontés. The cytotoxicity assessments revealed that Torrontés affected the viability of HT29-MTX and Caco-2 colon cancer cells by 70% and 50%, respectively, at the highest tested concentration (1 mg/mL). At the same time, both extracts did not demonstrate acute toxicity in Artemia salina or in red blood cell assays at 500 µg/mL. Both extracts inhibited the lipoxygenase enzyme (IC50: 154.7 and 784.7 µg/mL for Malbec and Torrontés), with Malbec also reducing the tyrosinase activity (IC50: 89.9 µg/mL), and neither inhibited the xanthine oxidase. The substantial phenolic content and diverse biological activities in the Calchaquí Valleys' pomaces underline their potentialities to be valorized for pharmaceutical, cosmetic, and food industries.

2.
Mult Scler Relat Disord ; 79: 105049, 2023 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-37864991

RESUMEN

BACKGROUND: Multiple sclerosis (MS) is an autoimmune neurodegenerative disease. Nutritional status influences the course of the disease, however, its relationship with sarcopenia needs further investigation. The aim of the study was to identify patients with sarcopenia and assess its association with nutritional status and the clinical course of the disease. METHODS: The study assessed 110 patients submitted to evaluation of sociodemographic characteristics, level of physical activity, nutritional status, and presence of sarcopenia. The clinical course of the disease, age at onset, disease duration, disease-modifying therapy, and expanded scale of disability status (EDSS) were investigated. RESULTS: Mean age was 37.17 (SD = 10.60) years, disease duration was 6.29 years (SD = 4.65), with a predominance of female gender (80.90 %), relapsing-remitting clinical form (RRMS) (89.10 %) and mild level of disability (EDSS median = 1.92). The group had excess weight (53.6 %) according to body mass index (BMI) and abdominal fat accumulation measured by waist circumference (WC) (53.6 %). High percentage of fat mass ( % FM) was observed in 54.5 % and 38.2 % of the patients according to bioimpedance (BIA) and ultrasound (US), respectively. It was observed that 15.5 % were at risk for sarcopenia, which was associated with excess weight, and high % FM (p<0.05). CONCLUSION: These findings highlight the importance of including nutritional status indicators, and sarcopenia assessment in the care of patients with MS.


Asunto(s)
Esclerosis Múltiple Recurrente-Remitente , Esclerosis Múltiple , Enfermedades Neurodegenerativas , Sarcopenia , Humanos , Femenino , Adulto , Masculino , Esclerosis Múltiple/complicaciones , Esclerosis Múltiple/epidemiología , Esclerosis Múltiple/tratamiento farmacológico , Sarcopenia/diagnóstico por imagen , Sarcopenia/epidemiología , Sarcopenia/etiología , Estado Nutricional , Progresión de la Enfermedad , Esclerosis Múltiple Recurrente-Remitente/complicaciones , Esclerosis Múltiple Recurrente-Remitente/epidemiología , Esclerosis Múltiple Recurrente-Remitente/tratamiento farmacológico
3.
Pharm Dev Technol ; 25(1): 54-67, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31535923

RESUMEN

This study aimed to prepare and characterize organogels containing microparticles of ascorbic acid (AA) obtained from propolis by-product. The formulations F1 (5% of microparticles) and F2 (10% of microparticles) were evaluated regarding rheological and textural properties, antioxidant and radical scavenging activity, in vitro release and cellular studies. The organogels showed plastic flow behavior and rheopexy. The textural parameters were within acceptable values for semisolid formulations. The antioxidant capacity of organogels F1 and F2 by the DPPH assay demonstrated IC50 ranging from 1523.59 to 1166.97 µg/mL, respectively. For the FRAP assay, the values found were 842.88 and 956.14 µmol of FSE/g formulation, respectively. Good scavenging activity against nitrogen species was observed. The concentration of 63 µg/mL did not present toxicity on HaCaT and HFF-1 cells. In vitro release profile of AA from organogels showed a slow pattern of drug release, mainly for F2. Therefore, the proposed organogel containing AA microparticles with propolis by-product matrix represents a promising platform for topical drug delivery with antioxidant effect.


Asunto(s)
Ácido Ascórbico/química , Geles/química , Própolis/química , Antioxidantes/química , Química Farmacéutica/métodos , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos/efectos de los fármacos , Viscosidad/efectos de los fármacos
4.
Rev Gaucha Enferm ; 40: e20180023, 2019 02 18.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-30785543

RESUMEN

OBJECTIVE: To understand the importance of group strategy in the process of nursing care to high-risk pregnant women. METHODS: Qualitative and descriptive study conducted at Assis Chateaubriand Teaching Maternity Hospital in February-March 2017, through focus groups with 24 hospitalized pregnant women. Data was submitted to content analysis and the relevant ideas of the discourses were extracted, forming categories. RESULTS: Three thematic categories were constructed: Interactive groups as a space of bond and coexistence, Educational strategy as an approach to good delivery and birthcare practices, Impact of group experience in strengthening the connection between the health team and the pregnant woman. The group of pregnant women is a space of coexistence and bonding that stimulates them to share knowledge and experiences, re-signifying links. The groups promote sharing of experiences, learning and reflection on the possibilities and limitations of the health-disease process, reducing anxiety and contributing to empowerment in decision making. CONCLUSION: The considerations and suggestions of the pregnant women guided the nurses in the development of the groups, which became strategic spaces of care, acquisition of knowledge and establishment of trust relationships with the nurses, with a consequent impact on the quality of care.


Asunto(s)
Educación en Salud , Embarazo de Alto Riesgo , Adulto , Femenino , Grupos Focales , Humanos , Embarazo , Adulto Joven
5.
Food Funct ; 10(2): 565-572, 2019 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-30702105

RESUMEN

Operations for sorting mushrooms at the industrial level usually generate large amounts of bio-residues not conforming to strict morphological criteria for commercial purposes, even though their biological content is not compromised. In this context, the present work aimed at evaluating the potential for reutilizing industrially discarded Agaricus blazei Murill (ABM). Thus, the content of essential nutrients and the chemical composition were determined, and MTT and LDH assays were used to evaluate the viability and cell death of Caco-2 and HT29 cell lines of an ethanolic extract prepared from ABM (preliminary safety tests for nutraceutical applications). The extract was incorporated into a semi-solid base cosmetic cream and cell viability effects of the extract, and of the final cream formulation, on a keratinocyte cell line (HaCaT) were studied (preliminary safety tests for cosmeceutical applications). Essential nutrients, such as proteins and carbohydrates, and a low fat content were determined for ABM. Twenty-two fatty acids were detected, with polyunsaturated fatty acids (PUFA) (∼53%) being the most abundant fraction. The cell viabilities of Caco-2 and HT29 cells were maintained up to 100 µg mL-1. After incorporation into the base cream, a formulation with a pale yellow colour and favourable pH was obtained. The cell viability of HaCaT cells in the presence of the extract and the final cream formulation was maintained in a concentration dependent manner, which indicates the safety of this extract for cosmeceutical applications. The results suggest that ABM residues can be used as an inexpensive and sustainable source of nutraceutical and cosmeceutical ingredients.


Asunto(s)
Agaricus/química , Cosmecéuticos/efectos adversos , Cosmecéuticos/análisis , Suplementos Dietéticos/efectos adversos , Suplementos Dietéticos/análisis , Brasil , Células CACO-2 , Supervivencia Celular , Células HT29 , Humanos
6.
Food Funct ; 9(8): 4518, 2018 08 15.
Artículo en Inglés | MEDLINE | ID: mdl-30019728

RESUMEN

Correction for 'Development of a microparticulate system containing Brazilian propolis by-product and gelatine for ascorbic acid delivery: evaluation of intestinal cell viability and radical scavenging activity' by Lizziane Maria Belloto de Francisco et al., Food Funct., 2018, DOI: 10.1039/c8fo00863a.

7.
Food Funct ; 9(8): 4194-4206, 2018 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-29993073

RESUMEN

The use of propolis by-product (PBP) microparticles (MP) as delivery systems can be a promising tool to surpass drawbacks related to low stability of ascorbic acid (AA). The objective of this study was to develop and characterize MP prepared with PBP containing AA. The MP was characterized regarding morphology, particle size, polydispersity index (PDI), association efficiency (AE), drug loading (DL), infrared and Raman spectroscopy as well as antioxidant and radical scavenging activity, in vitro release, and cellular studies. MP was shown to be spherical with some agglomeration. Its particle size was 1654 ± 0.210 nm with a PDI of 0.7. The AE and DL were, respectively, 100.30 ± 2.66% and 13.16 ± 0.59. Spectroscopic studies indicated a possible interaction between the PBP and AA. 2,2-Diphenyl-1-picrylhydrazyl (DPPH˙), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays demonstrated that the MP containing AA have an excellent antioxidant capacity as well as a considerable scavenging activity against reactive oxygen and nitrogen species. The in vitro release profile showed a slow pattern of drug release of AA from MP. Viability studies with intestinal cells revealed that MP did not present toxicity in Caco-2 and HT29-MTX. Moreover, AA could permeate Caco-2 monolayers and triple co-culture substantially at the end of 8 h, opposite to the MP. Therefore, the proposed MP formulation represents a promising platform for oral delivery of AA with a local effect on intestines.


Asunto(s)
Antioxidantes/farmacología , Ácido Ascórbico/farmacología , Células Epiteliales/efectos de los fármacos , Gelatina/química , Mucosa Intestinal/citología , Própolis/química , Antioxidantes/química , Ácido Ascórbico/química , Células CACO-2 , Células HT29 , Humanos , Microscopía Electrónica de Rastreo , Espectrofotometría Infrarroja , Espectrometría Raman
8.
J. Health Biol. Sci. (Online) ; 6(2): 165-169, 02/04/2018.
Artículo en Portugués | LILACS | ID: biblio-882713

RESUMEN

Introdução: Estudos demonstram haver limitações acerca do conhecimento sobre Cuidados Paliativos (CP), bem como a existência de dificuldade em discutir sobre o assunto com os profissionais de enfermagem Objetivo: descrever a experiência, enquanto acadêmicas de enfermagem, durante o desenvolvimento e a implementação de uma atividade de intervenção educativa à equipe de enfermagem sobre CP. Métodos: Trata-se de um estudo descritivo do tipo relato de experiência, realizado a partir do programa de iniciação científica de um centro universitário, como projeto de extensão acadêmica, no período de agosto de 2015 a julho de 2016. Resultados e Discussão: A atividade educativa possibilitou o desenvolvimento do pensamento crítico, tanto para as acadêmicas que realizaram o estudo e conduziram a intervenção, como para os participantes da pesquisa, que, durante toda a atividade, foram instigados a contribuir de forma ativa com o processo de aprendizagem. Essa intervenção educativa permitiu que os participantes se apropriassem de estratégias de planejamento, comunicação e organização do cuidado prestado ao paciente que necessita de CP. Acredita-se que a participação dos alunos de graduação em Enfermagem em projetos de iniciação científica favorece a formação de sujeitos capazes de reconhecer e intervir em problemas reais, em busca de transformações sociais que libertem e transformem o meio em que atuam. Conclusão: Conclui-se que, durante a formação acadêmica de enfermagem, a participação em projetos de iniciação científica, bem como o desenvolvimento de atividades que envolvem estratégias educativas dinâmicas pode propiciar um impacto positivo no conhecimento do aluno, favorecendo a formação de um profissional com pensamento crítico-reflexivo e influente em seu campo de atuação.


Introduction: Studies show that there are limitations of knowledge about Palliative Care (CP), as well as the difficulty of discussing this issue with nursing professionals. Objective: to describe the experience, as nursing students, during the development and implementation of an educational intervention activity to the nursing team about CP. Methods: This is a descriptive study of the type of experience report, carried out from the scientific initiation program of a University Center, as an academics extension project from August 2015 to July 2016. Results and Discussion: The activity Educational Development enabled the development of critical thinking, both for the students who conducted the study and led to the intervention, and for the participants in the research, who throughout the activity were encouraged to contribute actively to the learning process. This educational intervention allowed the participants to appropriate strategies for planning, communicating and organizing the care provided to the patient who needs CP. We believe that the participation of the Undergraduate Nursing students in scientific initiation projects favors the formation of subjects capable of recognizing and intervening in realistic problems in search for social transformations that will lead to liberate and transform the environment in which they act. Conclusion: during the academic nursing training, the participation in scientific initiation projects, as well as the development of activities involving dynamic educational strategies can provide a positive impact on the student's knowledge, favoring the formation of a professional with critical thinking -reflective and influential in his field of activity.


Asunto(s)
Educación en Salud , Cuidados Paliativos , Enfermería
9.
Food Res Int ; 105: 537-547, 2018 03.
Artículo en Inglés | MEDLINE | ID: mdl-29433245

RESUMEN

Propolis is a natural adhesive resinous compound produced by honeybees to protect hives from bacteria and fungi, being extremely expensive for food industry. During propolis production, a resinous by-product is formed. This resinous waste is currently undervalued and underexploited. Accordingly, in this study the proximate physical and chemical quality, as well as the antioxidant activity, radical scavenging activity and cell viability of this by-product were evaluated and compared with propolis in order to boost new applications in food and pharmaceutical industries. The results revealed that the by-product meets the physical and chemical quality standards expected and showed that the propolis waste contains similar amounts of total phenolic content (TPC) and total flavonoid content (TFC) to propolis. Also, a good scavenging activity against reactive oxygen and nitrogen species (ROS and RNS, respectively) determined by the assays of superoxide anion radical (O2-), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), nitric oxide (NO) and peroxyl radical (ROO) were determined. Linear positive correlations were established between the TPC of both samples and the antioxidant activity evaluated by three different methods (DPPH, ABTS and FRAP assays). The extracts were also screened for cell viability assays in two different intestinal cell lines (HT29-MTX and Caco-2), showing a viability concentration-dependent. Similarly, the Artemia salina assay, used to assess toxicity, demonstrated the concentration influence on results. Finally, the antifungal activity against ATCC species of Candida was demonstrated. These results suggest that propolis by-product can be used as a new rich source of bioactive compounds for different areas, such as food or pharmaceutical.


Asunto(s)
Antifúngicos/farmacología , Candida/efectos de los fármacos , Depuradores de Radicales Libres/farmacología , Mucosa Intestinal/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Própolis/farmacología , Residuos , Animales , Antifúngicos/toxicidad , Artemia/efectos de los fármacos , Brasil , Células CACO-2 , Candida/crecimiento & desarrollo , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Depuradores de Radicales Libres/toxicidad , Radicales Libres/química , Células HT29 , Humanos , Mucosa Intestinal/patología , Dosificación Letal Mediana , Própolis/toxicidad
10.
Biomed Pharmacother ; 96: 1230-1239, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-29174035

RESUMEN

The objective of this study was to verify a possible neuroprotective effect of the ethanolic extract of Erythrina velutina (EEEV). Male Swiss mice were submitted to transient cerebral ischemia by occlusion of both carotid arteries for 30 min and treated for 5 days with EEEV (200 or 400 mg/kg) or Memantine (MEM) 10 mg/kg, with initiation of treatment 2 or 24 h after Ischemia. On the 6th day after the induction of ischemia, the animals were submitted to evaluation of locomotor activity and memory and then sacrificed. The brains were dissected for the removal of the prefrontal cortex (PFC), hippocampus (HC) and striatum (ST) for determination of amino acid concentrations. In the step down and Y-maze tests, ischemia caused damage to the animals and treatment with EEEV or MEM reversed this effect. The animals submitted to ischemia also showed memory deficit in the object recognition test, an effect that was reverted by EEEV400 and MEM10. Amino acid dosage showed an increase in excitatory amino acid concentrations in the PFC of the ischemic animals and this effect was reversed by the treatment with EEEV400/24H. Regarding the inhibitory amino acids, ischemia caused an increase of taurine in the PFC while treatment with MEM10/24H or EEEV400/24H reversed this effect. In HC, an increase in excitatory amino acids was also observed in ischemiated animals having treatment with EEEV200/2H or EEEV400/24H reversed this effect. Similar effect was also observed in the same area in relation to the inhibitory amino acids with treatment with MEM10/24H or EEEV400/24H. In the ST, ischemia was also able to cause an increase in excitatory amino acids that was reversed more efficiently by the treatments with MEM10/24H and EEEV200. Also in this area, an increase of taurine and GABA was observed and only the treatment with EEEV200/2H showed a reversion of this effect. In view of these findings, EEEV presents a neuroprotective effect possibly due to its action on amino acid concentrations, and is therefore a potential therapeutic tool in reducing the damage caused by ischemia.


Asunto(s)
Isquemia Encefálica/tratamiento farmacológico , Erythrina/química , Extractos Vegetales/farmacología , Aminoácidos/metabolismo , Animales , Encéfalo/efectos de los fármacos , Isquemia Encefálica/metabolismo , Infarto Cerebral/tratamiento farmacológico , Infarto Cerebral/metabolismo , Etanol/química , Masculino , Memoria/efectos de los fármacos , Ratones , Fármacos Neuroprotectores/farmacología
11.
J Med Food ; 20(10): 1039-1046, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28749738

RESUMEN

Alfalfa (Medicago sativa) has been extensively used as animal feed, due to its fiber, protein, minerals, and vitamins, being also a useful source of phenolic compounds with potential therapeutic benefits. Nevertheless, its potential use as human ingredient is scarce. The aim of this work was to assess the nutritional composition, amino acid profile, and antioxidant capacity (AOC) of freeze-dried juice (FDJ) and fibrous residual material (RM), two new alfalfa-derived products (Adps) recently launched as ingredients for beverage preparations. Results demonstrated a high content of proteins (23-30 g/100 g FDJ and 13-17 g/100 g RM), crude fiber (29 g/100 g RM), and minerals (such as sodium, calcium, iron, and zinc). No significant difference was found in caloric content (4 kcal/g). Essential and nonessential amino acids were quantified in both Adps being leucine and lysine the most abundant. Total phenolic and total flavonoid contents (TPC and TFC, respectively) and their changes along the different harvesting periods of the year were also examined. FDJ presented the highest TPC in May (19 mg gallic acid equivalents/g dry weight [dw]), while in October TFC had the maximum value (4 mg catechin equivalents/g dw). Both products exhibited an interesting AOC by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power assays. This study reports the nutraceutical potential of two new types of Adps.


Asunto(s)
Bebidas/análisis , Suplementos Dietéticos/análisis , Medicago sativa/química , Extractos Vegetales/análisis , Aminoácidos/análisis , Antioxidantes/análisis , Proteínas de Plantas/análisis
12.
Reprod. clim ; 32(2): 78-84, 2017. tab
Artículo en Portugués | LILACS | ID: biblio-883261

RESUMEN

Objetivos: analisar a violência institucional contra mulheres no processo de parturição em maternidades vinculadas a Rede Cegonha de Fortaleza/Cascavel. Material e Métodos: Trata­se de um estudo descritivo com abordagem quantitativa, realizado em onze hospitais­maternidades, de média e alta complexidade da rede de atenção à saúde da mulher no estado do Ceará, composta por 11 hospitais­maternidades, participaram da pesquisa 3.765 puérperas de parto transpelviano que se encontravam nas unidades de alojamento conjunto das referidas instituições. Resultados: Sobre as características sociodemográficas das participantes da pesquisa tinham entre 20 a 34 anos (62,1%), tratando­se do suporte emocional que deve ser oferecido durante o parto, as participantes relataram que tiveram a presença de visitas (82,9%), em relação as atitudes que ocasionaram sentimentos de hesitação as participantes afirmaram que sentiam segurança (89,4%). Conclusões: A pesquisa demostra a importância de um atendimento humanizado adequado através da estruturação e organização da atenção materno­infantil proposto pela rede cegonha.(AU)


Objectives: to analyze the institutional violence against women in the parturition process in maternity hospitals linked to Stork Network of Fortaleza/Cascavel. Material and Methods: This is a descriptive study with a quantitative approach, performed in eleven maternities, medium and high complexity of the network of health care of women in the state of Ceará, composed of 11 maternity hospitals, participated in the survey 3.765 transpelvic birth mothers who were in the accommodation units set of these institutions. Results: About the sociodemographic characteristics of the study participants were between 20­34 years (62.1%), in the case of emotional support that should be offered during delivery, the participants reported that they felt the presence of visits (82.9%) in relation to the attitudes that led to feelings of hesitation the participants said they felt security (89.4%). Conclusions: The study demonstrates the importance of an appropriate humanized care through the structure and organization of maternal and child care proposed by the stork network.(AU)


Asunto(s)
Humanos , Femenino , Embarazo , Adulto , Adulto Joven , Parto Humanizado , Bienestar Materno , Servicios de Salud Materno-Infantil , Seguridad del Paciente , Salud Pública , Violencia contra la Mujer
13.
J Med Food ; 19(10): 970-977, 2016 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30913400

RESUMEN

Aerial parts of Medicago sativa L. have been used as food and its consumption has been associated with health benefits, one among the most important being menopausal symptoms control. This work was aimed to explore possible pharmacological effects of two new alfalfa-derived products that have recently emerged as daily beverage preparations. In exploring their potential estrogenic effects, they produced no relevant alteration in the uterus. However, lowering glucose levels until normal values without causing further hypoglycemic effect were observed, when rats were treated with 1.5 g/kg/day samples. In vivo acute toxicity was not found when the alfalfa products were tested up to 3 g/kg rat weight. Furthermore, in vitro studies were conducted to assess their possible toxic effects. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase tests were carried out on the Caco-2 cell model to determine cell viability and membrane integrity. A concentration-dependent effect was observed, with a significant decrease in cell viability after exposure to concentrations of alfalfa product up to 100 mg/mL (after 3 h of incubation) and 50 mg/mL (after 24 h of treatment). Although in vitro level, the decrease in cell viability at these still low doses may underlie some toxicity, making necessary additional studies before any recommendation of a sustained consumption of these products by humans.

14.
Artículo en Inglés | MEDLINE | ID: mdl-24023579

RESUMEN

This study aimed to investigate behavioral and neurochemical effects of α -lipoic acid (100 mg/kg or 200 mg/kg) alone or associated with L-DOPA using an animal model of Parkinson's disease induced by stereotaxic injection of 6-hydroxydopamine (6-OHDA) in rat striatum. Motor behavior was assessed by monitoring body rotations induced by apomorphine, open field test and cylinder test. Oxidative stress was accessed by determination of lipid peroxidation using the TBARS method, concentration of nitrite and evaluation of catalase activity. α -Lipoic acid decreased body rotations induced by apomorphine, as well as caused an improvement in motor performance by increasing locomotor activity in the open field test and use of contralateral paw (in the opposite side of the lesion produced by 6-OHDA) at cylinder test. α -lipoic acid showed antioxidant effects, decreasing lipid peroxidation and nitrite levels and interacting with antioxidant system by decreasing of endogenous catalase activity. Therefore, α -lipoic acid prevented the damage induced by 6-OHDA or by chronic use of L-DOPA in dopaminergic neurons, suggesting that α -lipoic could be a new therapeutic target for Parkinson's disease prevention and treatment.

15.
Oxid Med Cell Longev ; 2012: 697541, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23251721

RESUMEN

This work was designed to study MCT effect in histopathological analysis of hippocampus (HC) and parahippocampal cortex (PHC) and in oxidative stress (OS) parameters in brain areas such as hippocampus (HC), prefrontal cortex (PFC), and striatum (ST). Swiss mice (25-30 g) were administered a single i.p. dose of MCT (5, 50, or 100 mg/kg) or 4% Tween 80 in saline (control group). After 30 minutes, the animals were sacrificed by decapitation and the brain areas (HC, PHC, PFC, or ST) were removed for histopathological analysis or dissected and homogenized for measurement of OS parameters (lipid peroxidation, nitrite, and catalase) by spectrophotometry. Histological evaluation of brain structures of rats treated with MCT (50 and 100 mg/kg) revealed lesions in the hippocampus and parahippocampal cortex compared to control. Lipid peroxidation was evident in all brain areas after administration of MCT. Nitrite/nitrate content decreased in all doses administered in HC, PFC, and ST. Catalase activity was increased in the MCT group only in HC. In conclusion, monocrotaline caused cell lesions in the hippocampus and parahippocampal cortex regions and produced oxidative stress in the HC, PFC, and ST in mice. These findings may contribute to the neurological effects associated with this compound.


Asunto(s)
Encéfalo/efectos de los fármacos , Encéfalo/patología , Monocrotalina/toxicidad , Oxidantes/toxicidad , Animales , Encéfalo/enzimología , Caspasa 3/metabolismo , Catalasa/metabolismo , Cuerpo Estriado/efectos de los fármacos , Cuerpo Estriado/metabolismo , Cuerpo Estriado/patología , Hipocampo/efectos de los fármacos , Hipocampo/enzimología , Hipocampo/patología , Inmunohistoquímica , Peroxidación de Lípido/efectos de los fármacos , Masculino , Ratones , Monocrotalina/administración & dosificación , Nitritos/metabolismo , Estrés Oxidativo/efectos de los fármacos , Corteza Prefrontal/efectos de los fármacos , Corteza Prefrontal/metabolismo , Corteza Prefrontal/patología , Ratas , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo
16.
Mossoró; s.n; 01/08/2012. 72 p. ilus.
Tesis en Portugués | VETINDEX | ID: biblio-1505037

RESUMEN

A lagosta tem papel de destaque no setor pesqueiro nacional, sendo o principal recurso pesqueiro da região Nordeste e 95% desta produção destina-se ao mercado internacional. O grau de frescor do pescado é um dos aspectos mais importantes para determinar a qualidade do pescado, e frequentemente se recorre a métodos sensoriais, físicos, bioquímicos e microbiológicos. O objetivo deste trabalho foi avaliar a qualidade da lagosta inteira armazenada em gelo através do Método de Índice de Qualidade (MIQ), que permite a determinação rápida e eficiente do grau de frescor do pescado. Os pontos de deméritos foram designados aos atributos sensoriais selecionados de acordo com sua importância. Durante o armazenamento foram realizadas determinações do Nitrogênio das Bases Voláteis Totais (NBVT), pH e análises microbiológicas (Coliformes Termotolerantes, Salmonella spp. e Staphylococcus aureus). O Índice de Qualidade (IQ) variou de zero (máximo frescor) a 14 (perda total do frescor) atingindo o limite de aceitabilidade para consumo (IQ = 6,3), o que equivale a um período de 7 dias. O valor do N-BVT variou de 19,69 a 29,63 mg 100g-1, e o pH variou de 6,91 a 7,03 no primeiro e 17º dia de armazenamento, respectivamente. Os resultados microbiológicos atenderam aos padrões microbiológicos estabelecidos pela legislação brasileira.


The lobster has an important role in the national fisheries sector, the main fishing resourc e in the Northeast and 95% of this production is intended for the international market. The seafood freshness degree is one of the most important in determining the seafood quality, and often uses a sensorial, physical, biochemical and microbiological methods. The objective of this study was to evaluate the whole lobster quality stored on ice through the Quality Index Method (QIM), which allows rapid and efficient determination of the seafood freshness. The demerits points were assigned to the sensory attributes selected according to their importance. During storage the total volatile bases nitrogen (TVB - N), pH and microbiological (fecal coliform, Salmonella spp. and Staphylococcus aureus ) were made. The Quality Index (IQ) ranged from zero (maximum freshness) to 14 (total loss of freshness) reached the acceptable limit for consumption (IQ = 6.3), which corresponds to a period of 7 days. The value of the TVB - N ranged from 19.69 to 29.63 mg 100g - 1 , and the pH ranged from 6.91 to 7.03 in the first and 17th day of storage, respectively. The microbiological results met the microbiological standards e stablished by Brazilian legislation.


Asunto(s)
Animales , Alimentos Enfriados , Palinuridae/fisiología , Calidad de los Alimentos , Técnicas Microbiológicas/métodos , Almacenamiento de Alimentos , Conservación de Alimentos
17.
Esc. Anna Nery Rev. Enferm ; 11(1): 86-91, mar. 2007. ilus, tab
Artículo en Portugués | LILACS, BDENF - Enfermería | ID: lil-570792

RESUMEN

A fototerapia é o tratamento inicial da icterícia neonatal. Objetivamos conhecer a percepção da mãe acerca da fototerapia e identificar as suas dificuldades, relacionadas ao tratamento fototerápico. Estudo descritivo com abordagem qualitativa, realizado no Alojamento Conjunto (AC) de um hospital público em Fortaleza-CE, com 8 puérperas na faixa etária entre 13 e 25 anos. Os dados foram coletados em maio e junho/2006, mediante entrevista com duas questões de pesquisa: O que representa para a senhora ver o seu filho sob fototerapia; quais são as dificuldades enfrentadas em relação aos cuidados com o seu bebê na fototerapia? Na análise das falas, identificamos as categorias: percepção da mãe com relação ao cuidado com o bebê sob a fototerapia, dificuldades enfrentadas pela mãe com seu filho em fototerapia, e a mãe após as orientações recebidas. Concluímos que existe a necessidade de repensar as orientações e assumir autenticamente os cuidados de enfermagem ao binômio mãe-filho.


Asunto(s)
Humanos , Femenino , Embarazo , Adolescente , Adulto , Recién Nacido , Ictericia Neonatal/enfermería , Enfermería Neonatal , Fototerapia/enfermería , Alojamiento Conjunto , Hospitales Públicos
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