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1.
Rev. argent. cir. plást ; 30(2): 104-115, 20240000. fig
Artículo en Español | LILACS, BINACIS | ID: biblio-1566499

RESUMEN

El tratamiento del envejecimiento facial debe ser tratado en su conjunto. Elegimos la técnica DPFL (Deep Plane Facelift) por ser la más efectiva en el tratamiento del tercio medio e inferior, la liberación de los ligamentos de retención facial permite una reposición vertical sin energía en los tejidos. La no deslaminación del complejo SMAS-Platisma conforma un colgajo compuesto de mayor estabilidad en las suturas y mayor trofismo cutáneo. Como procedimientos complementarios incluimos el lifting fronto-temporal extendido con incisión mínima, blefaroplastia, lipofilling facial, lifting de labio, rinoplastia, lobuloplastia auricular y cuello profundo. Esta sinergia quirúrgica optimiza el resultado siendo más natural y armónico, conservando la fisonomía de los 10 a 15 años anteriores de los pacientes, hecho observado en fotografías.


The treatment of facial aging must be treated as a whole. We chose the DPFL (Deep Plane Facelift) technique because it is the most eff ective in the treatment of the middle and lower 1/3, the release of the facial retaining ligaments allows a vertical repositioning without energy in the tissues. The non-delamination of the SMAS-Platysma complex forms a composite fl ap with greater suture stability and greater skin trophism. As complementary procedures we include the extended fronto-temporal lift with minimal incision, blepharoplasty, facial lipofi lling, lip lift, rhinoplasty, auricular lobuloplasty, deep neck. This surgical synergy optimizes the result being more natural and harmonic, preserving the physiognomy of 10 to 15 years ago of the patients, fact observed in photographs.


Asunto(s)
Humanos , Masculino , Femenino , Ritidoplastia/métodos , Envejecimiento de la Piel/fisiología , Procedimientos de Cirugía Plástica/métodos
2.
Rev. argent. cir. plást ; 30(2): 116-120, 20240000. fig
Artículo en Español | LILACS, BINACIS | ID: biblio-1566513

RESUMEN

El lifting de plano profundo es una técnica de rejuvenecimiento facial que nos permite lograr resultados altamente satisfactorios aun en casos de difícil resolución. El conocimiento detallado de la anatomía facial es de primordial importancia para su aprendizaje y perfeccionamiento. Es una técnica que insume mayor tiempo operatorio, ya que requiere una adecuada liberación de ligamentos faciales y cervicales para la correcta movilización de los tejidos y su reposicionamiento sin tensión, pero al mismo tiempo es segura y confi able.


Deep plane lifting is a rejuvenation technique. facial treatment that allows us to achieve highly satisfactory even in cases of diffi cult resolution. He met Detailed understanding of facial anatomy is of primary importance importance for your learning and improvement. It is a technique that requires more operating time, since it requires adequate release of ligaments facial and cervical for the correct mobilization of the tissues and their repositioning without tension, but at the same time time is safe and reliable.


Asunto(s)
Humanos , Femenino , Ritidoplastia/métodos , Disección/métodos , Cara/anatomía & histología
3.
Rev. argent. cir. plást ; 30(2): 152-161, 20240000. fig, tab
Artículo en Español | LILACS, BINACIS | ID: biblio-1567237

RESUMEN

La parálisis facial periférica es una condición que impacta negativamente en la calidad de vida y psiquis de los pacientes. Se aborda como una afección relativamente frecuente, con diversas causas, con un manejo multidisciplinario para su tratamiento. Se presenta un estudio retrospectivo de cuatro pacientes femeninas que sufrieron parálisis facial periférica crónica, causada por cirugías previas. Se emplearon técnicas estáticas y dinámica para su tratamiento, incluyendo una nueva técnica estática no descrita anteriormente para parálisis faciales basada en el lifting de tercio medio de plano profundo. Se evaluó la satisfacción de los pacientes mediante el cuestionario FACE Q pre- y posoperatorio, mostrando resultados positivos en ambos grupos de tratamiento. Se destaca la importancia de la kinesiología motora en el proceso de rehabilitación. Demostramos la efi cacia de técnicas accesibles y de bajo costo, así como la introducción exitosa de técnicas más complejas, como la transferencia muscular con anastomosis nerviosa.


Peripheral facial paralysis is a condition that negatively impacts the quality of life and psyche of patients. It is addressed as a relatively frequent condition with various causes, managed through a multidisciplinary approach for its treatment. A retrospective study is presented involving four female patients who suff ered from chronic peripheral facial paralysis caused by previous surgeries. Both static and dynamic techniques were used for their treatment, including a new static technique not previously described for facial paralysis based on deep plane midface lifting. Patient satisfaction was evaluated using the FACE Q questionnaire pre- and post-operatively, showing positive results in both treatment groups. The importance of motor kinesiology in the rehabilitation process is highlighted. We demonstrate the effi cacy of accessible and low-cost techniques, as well as the successful introduction of more complex techniques, such as muscle transfer with nerve anastomosis.


Asunto(s)
Humanos , Femenino , Rehabilitación/métodos , Elevación , Parálisis Facial/terapia
5.
Aesthetic Plast Surg ; 47(5): 1851-1858, 2023 10.
Artículo en Inglés | MEDLINE | ID: mdl-37592144

RESUMEN

BACKGROUND: Eyebrow tail ptosis is usually associated with dermatochalasis of the upper eyelid, and it is necessary to treat them together. For these associated procedures to be incorporated as a routine in upper eyelid surgeries, they should preferably be less invasive with consequent fewer complications. OBJECTIVE: We describe a minimally invasive technique for the correction of mild-to-moderate eyebrow tail ptosis corrected together with superior dermatochalasis through the blepharoplasty incision. METHODS: A phase 2 clinical trial was conducted from February 2020 to December 2021. A total of 50 patients underwent conventional upper blepharoplasty surgery associated with the proposed eyebrow lift technique: internal pexia in the periosteum, dissection, and posterior fixation of the orbicularis muscle to the arcus marginalis, removal of the lateral part of the orbicularis muscle, and loosening of the orbital retention ligament. Evaluation of the height of the eyebrow tail was performed with digital photography in the initial consultation and at 30-90 days postoperatively and analyzed with ImageJ software. RESULTS: Mean difference in brow height at 1-month and 3-month postoperative evaluations compared to the preoperative period was 3.45-3.33 mm, respectively. CONCLUSION: Our study demonstrated a minimally invasive surgical technique for the treatment of mild-to-moderate eyebrow ptosis with significant eyebrow tail lift results that remained stable during the study period. LEVEL OF EVIDENCE II: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .


Asunto(s)
Blefaroplastia , Ritidoplastia , Herida Quirúrgica , Humanos , Blefaroplastia/métodos , Estudios Prospectivos , Estudios Retrospectivos , Párpados/cirugía , Ritidoplastia/métodos , Cejas , Herida Quirúrgica/cirugía
6.
Australas J Dermatol ; 64(3): 322-329, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37265044

RESUMEN

Surgical facelifts and noninvasive techniques such as mesotherapy, hyaluronic fillers and botulinum toxin, among other procedures are widely used nowadays to reverse skin ageing and achieve rejuvenation effects. Secondary alopecia due to cosmetic procedures is a rare side effect and is poorly described in the literature. The mechanisms in which hair loss develops after an aesthetical procedure or surgical technique are not well known. The development of secondary scarring alopecia or non-scarring alopecia depends upon the interaction between different external factors, the individual host response and the extent of damage to the hair follicle anatomy. Current knowledge hints at the type of substance used, pressure to hair structures and vasculature due to the materials used, previously unknown or unaware hair disorder, and poor surgical techniques to be the main factors contributing to the development of secondary alopecia. Physicians and patients must be aware of all the substances used for the procedures and be attentive to any change in hair density or hair loss. Clinicians must have a low threshold to take biopsies if the risk of scarring alopecia may occur. Herein, we review the clinical, trichoscopic, histopathological findings and potential pathophysiological mechanisms of hair loss due to different aesthetic procedures.


Asunto(s)
Alopecia , Ritidoplastia , Humanos , Alopecia/patología , Cabello , Folículo Piloso/patología , Biopsia/efectos adversos
7.
J Cosmet Dermatol ; 22(8): 2158-2165, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37021458

RESUMEN

BACKGROUND: Thread lifting procedures for facial rejuvenation have made great strides with the development of absorbable threads. Although they have gained fame among plastic surgeons and dermatologists, there have been very few studies published in scientific articles on the effects of absorbable threads in facial rejuvenation, as well as few studies published by aesthetic physicians. The most effective and efficient techniques to determine the adequate place to insert the reabsorbable thread, and the different ways to evaluate the effectiveness of these aesthetic procedures, are still unknown. OBJECTIVES: This review aims to identify, in the scientific literature, the evaluation techniques for a safe and correct insertion of PDO threads in facial rejuvenation procedures. METHODOLOGY: A review of scientific literature was performed using the following list of keywords, descriptors, or thesauri: PDO threads, aesthetics, facial rejuvenation. Scopus, PubMed, and Web of Science databases were used for the literature search. Articles from years 2012-2022 were selected. The reference lists of the identified articles were included. (16) out of (35) articles associated with the topic were selected. Simple and compound searches were performed with the keywords and very few rigorous studies about the use of PDO threads for aesthetic procedures were found. RESULTS: Very few rigorous scientific studies on the use of PDO threads for facial rejuvenation procedures were found. There is a strong theoretical and methodological gap on this subject, and, also, on the evaluation techniques that could be used for the safe and correct insertion of the threads. CONCLUSION: The bibliographic information found shows a huge theoretical and methodological gap in the subject of procedures for facial rejuvenation with PDO threads, as well as the techniques and tools employed to guarantee the correct insertion of the threads.


Asunto(s)
Técnicas Cosméticas , Cara , Humanos , Estética , Rejuvenecimiento , Ritidoplastia/métodos
8.
Facial Plast Surg Aesthet Med ; 25(6): 494-499, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36719989

RESUMEN

Background: This study aims to evaluate three-dimensional stereophotogrammetry measured volume changes in the tear trough area after lower blepharoplasty with midface lift with or without orbital fat manipulation. Objective: Among patients undergoing midface lift surgery, volume change by three-dimensional photography in the tear trough region was compared between those having no orbital fat manipulation, fat transposition, or fat resection. Methods: This is a prospective study of 260 eyelids of 130 patients who underwent cosmetic midface surgery. All patients had an external skin muscle flap midface lift. During the procedure, medial inferior orbital fat was resected, repositioned to a preperiosteal dissection pocket, or not manipulated. Forty-six patients (35.4%) had no fat manipulation, 42 patients (32.3%) had fat preservation with repositioning to the preperiosteal pockets on the medial inferior orbital rim, and 42 patients (32.3%) had fat resection without repositioning. Results: All patients had volume gain in tear trough volume. The amount was not statistically significantly different between fat treatment groups. Mean follow-up time was 12 months (range 6-20 months). Conclusions: Midface lifting improved tear trough volume irrespective of how fat was manipulated.


Asunto(s)
Blefaroplastia , Ritidoplastia , Humanos , Blefaroplastia/métodos , Ritidoplastia/métodos , Estudios Prospectivos , Párpados/cirugía , Cara/cirugía
9.
J Cosmet Dermatol ; 22(3): 804-809, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36465022

RESUMEN

BACKGROUND: Aging is part of the immutable characteristics of the human being, and the search for treatments to alleviate the undesirable effects of aging is frequent in the dermatological office. AIMS: This work proposes the technique of association of PDO threads with technologies and the description of the facial treatment protocols. METHODS: For contextualization, two case studies of two patients subjected to the procedure are analyzed. CONCLUSIONS: In recent years, there have been marked changes in facial rejuvenation techniques using PDO threads and these have shown satisfactory results for collagen biostimulation. Having technologies in dermatological clinics is a frequent reality, and sometimes, some doctors are in doubt about when to use them associated with PDO wires and in which sequence it should be done, ensuring patient safety and maintaining the integrity of the wires. This article aims to bring clarity about how to use technology and when is the right time. It is also intended to clarify that the PDO wire can be used in conjunction with the microfocused ultrasound technology, since the melting point of the PDO polymer is between 110° and 115°C.


Asunto(s)
Ritidoplastia , Envejecimiento de la Piel , Humanos , Ritidoplastia/métodos , Polidioxanona/efectos adversos , Cara , Envejecimiento , Rejuvenecimiento , Protocolos Clínicos
10.
Ann Chir Plast Esthet ; 68(2): 173-179, 2023 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-36543616

RESUMEN

Neurofibromatosis is an autosomal dominant disorder characterized by tumors of the nervous system and skin. Plexiform neurofibromas are common complications of neurofibromatosis type 1 and can cause large facial deformities. Vascular anomalies are in turn a rare manifestation of neurofibromatosis. We present the case of a 48-year-old female patient with right hemifacial neurofibromatosis associated with venous vascular malformation, previously treated surgically and then with sclerosing agents, determining severe residual facial deformity. Her surgical approach using a modified facelift technique associated with partial tumor debulking and lipofilling seems to be a valid technical alternative for these highly complex cases that require a customized approach after exhaustive preoperative evaluation.


Asunto(s)
Neurofibroma Plexiforme , Neurofibromatosis 1 , Ritidoplastia , Malformaciones Vasculares , Humanos , Femenino , Persona de Mediana Edad , Neurofibromatosis 1/complicaciones , Neurofibromatosis 1/cirugía , Neurofibromatosis 1/patología , Neurofibroma Plexiforme/complicaciones , Neurofibroma Plexiforme/cirugía , Neurofibroma Plexiforme/patología , Malformaciones Vasculares/cirugía , Malformaciones Vasculares/complicaciones , Cuidados Preoperatorios
11.
São José dos Campos; s.n; 2023. 62 p. ilus, tab.
Tesis en Portugués | BBO - Odontología | ID: biblio-1427419

RESUMEN

O objetivo deste estudo foi analisar a resistência de união de restaurações indiretas cimentadas em superfícies dentinárias desmineralizadas como simulação da remoção seletiva de dentina cariada. Foram utilizados 40 dentes, os quais as superfícies foram planificadas até que a dentina fosse exposta. Os dentes foram divididos em quatro grupos: o primeiro é formado por dentes com dentina sadia, o segundo por aqueles que passaram pelo protocolo de desmineralização de sua dentina. O terceiro grupo de espécimes, além de receberem a desmineralização de sua dentina, receberam selamento da dentina com resina fluida. E o quarto grupo recebeu camada de cimento de ionômero de vidro (CIV) sobre a dentina desmineralizada. Em seguida, peças em dissilicato de lítio foram cimentadas individualmente na região condicionada de cada amostra. O adesivo utilizado foi o autocondicionante (Single Bond Universal; 3M), com duas aplicações ativas. O cimento usado foi o RelyX Ultimate (3M). As peças foram cimentadas e a força de cimentação foi padronizada. Somente após a cimentação, o conjunto recebeu fotoativação com aparelho Bluephase (Ivoclar Vivadent, Liechtenstein), com emitância de 1200 mW/cm², por 60 segundos em cada lado. Após 48 horas da cimentação, os espécimes foram submetidos aos testes mecânicos de tração, com velocidade de 1 mm/min. A análise do tipo de fratura foi realizada por estereomicroscopia. Os dados foram convertidos em MPa. Os grupos controle, desmineralizado e SDI não apresentaram diferenças estatísticas significativas. O grupo do CIV apresentou diferenças estatísticas em relação aos grupos com dentina desmineralizada e que recebeu a resina flow, porém não foi observada diferenças estatisticamente significantes entre os valores do grupo controle e que recebeu CIV (AU)


The aim of this study was to analyze the bond strength of cemented indirect restorations on demineralized dentin surfaces as a simulation of selective removal of decayed dentin. 40 teeth were used, whose surfaces were flattened until the dentin was exposed. The teeth were divided into four groups: the first is formed by teeth with healthy dentin, the second by those who have gone through the protocol of demineralization of their dentin. The third group of specimens, in addition to receiving demineralization of their dentin, received dentin sealing with fluid resin. And the fourth group received a layer of glass ionomer cement over the demineralized dentin. Then, pieces of lithium disilicate were cemented individually in the conditioned region of each sample. The adhesive used was self-etching (Single Bond Universal; 3M), with two active applications. The cement used was RelyX Ultimate (3M). The pieces were cemented, and the cement strength was standardized. Only after cementation, the set received photoactivation with a Bluephase device (Ivoclar Vivadent, Liechtenstein), with an emittance of 1200 mW/cm², for 60 seconds on each side. After 48 hours of cementation, the specimens were submitted to mechanical tensile tests with a speed of 1 mm/min. An analysis of the type of fracture was performed by stereomicroscopy. The data was converted to MPa. The control, demineralized and IDS groups did not show statistically significant differences. The GIC group showed statistical differences in relation to the groups with demineralized dentin and the one that received the flow resin. However, no statistically significant differences were observed between the values of the control group and the GIC one (AU)


Asunto(s)
Cementación , Elevación , Dentina , Fracturas Óseas
12.
Facial Plast Surg ; 38(6): 650-667, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36563674

RESUMEN

Establishing a pleasant cervical contour in short-neck patients is of one the most difficult challenges for a facial plastic surgeon. Subplatysmal volume reduction, platysma tightening, and skin distribution adequately coupled with the middle third facelift are the pillars of the surgical approach. Additionally, treating the small chin, which is frequently observed in these patients, improves the overall result. In this paper, an objective method to define short-neck patients is offered. The applied surgical anatomy of the neck is revised. Innovative strategies to treat the supra and infrahyoid subplatysmal structures are presented, including the sternohyoid muscles plicature and the use of the interplatysmal/subplatysmal fat flap. A novel chin augmentation technique, using a subperiosteal en bloc fat graft is also introduced. A modified deep plane approach is described, including a continuous suture of the middle third fasciocutaneous flap. A combined lateral platysma tensioning with the sternocleidomastoid rejuvenation is demonstrated. Lastly, the hemostatic net is revisited as a critical approach to resolve the defying skin accommodation.


Asunto(s)
Procedimientos de Cirugía Plástica , Ritidoplastia , Humanos , Cuello/cirugía , Cuello/anatomía & histología , Músculos del Cuello/cirugía , Músculos del Cuello/anatomía & histología , Ritidoplastia/métodos , Colgajos Quirúrgicos
13.
J. health med. sci. (Print) ; 8(2): 125-130, abr.-jun. 2022. tab
Artículo en Español | LILACS | ID: biblio-1391951

RESUMEN

INTRODUCCIÓN: La mayoría de los establecimientos educacionales tienen una jornada escolar completa, generando que el estudiante transporte mayor cantidad de útiles escolares en su mochila. OBJETIVO: Analizar el peso promedio de las mochilas de espalda en estudiantes de educación básica de la comuna de Copiapó. METODOLOGÍA: se realizó un estudio descriptivo de corte transversal con alcance correlacional, la muestra estuvo constituida por 286 estudiantes (139 varones y 147 mujeres) de educación básica, de un establecimiento escolar de la comuna de Copiapó. Se analizó el peso corporal, la altura y el peso de las mochilas durante una semana, mediante una plataforma de fuerza y estadiómetro. RESULTADOS: Se encontró que entre los días lunes a jueves los estudiantes llevaban en su mochila un peso que sobrepasaba el 10/ de su peso corporal. Asimismo, las mochilas de espaldas son las más utilizadas en comparación con las mochilas con ruedas, con un 88,1% de la muestra. Sin embargo, las mochilas de espalda presentaron menor peso al compararlas con las mochilas con ruedas, las cuales tuvieron valores cercanos al 20% del peso corporal. CONCLUSIÓN: La mayoría de los estudiantes transportaban en sus mochilas, pesos que superan el 10% de peso recomendado, siendo las mochilas con rueda las de mayor peso. Se recomienda desarrollar normas establecidas dentro de los colegios, para evitar el traslado de dichas cargas y prevenir alteraciones posturales, biomecánicas y fisiológicas.


INTRODUCTION: Most educational establishments have a full school day, causing the student to carry a greater number of school supplies in their backpack. OBJECTIVE: To analyze the average weight of backpacks in basic education students of the Copiapó district. METHODOLOGY: a descriptive cross-sectional study with correlational scope was carried out, the sample consisted of 286 students (139 men and 147 women) of basic education, from a school in the district of Copiapó. Body weight, height and backpack weight were analyzed for one week, using a force platform and stadiometer. RESULTS: It was found that between Monday and Thursday the students carried a weight in their backpack that exceeded 10% of their body weight. Likewise, backpacks on the back are the most used compared to backpacks with wheels, with 88.1% of the sample. However, the backpacks presented less weight when compared to the backpacks with wheels, which had values close to 20% of body weight. CONCLUSION: Most of the students carried weights in their backpacks that exceed 10% of the recommended weight, with wheeled backpacks being the heaviest. It is recommended to develop established norms within the schools, to avoid the transfer of said loads and prevent postural, biomechanical and physiological alterations.


Asunto(s)
Humanos , Masculino , Femenino , Niño , Estudiantes , Levantamiento de Peso/fisiología , Elevación , Peso Corporal , Chile , Estudios Transversales
14.
Nurs Open ; 9(5): 2304-2313, 2022 09.
Artículo en Inglés | MEDLINE | ID: mdl-35609217

RESUMEN

AIM: To investigate the level of self-reported work ability and its association with manual patient handling in healthcare workers. DESIGN: Cross-sectional study adhering to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. METHODS: A total of 320 healthcare workers answered a self-administered questionnaire regarding manual patient handling, work ability, occupational factors, occurrence of low back pain and sociodemographic and lifestyle factors from November 2016 to March 2017. The association between manual patient handling and low back pain was analysed with Poisson regression models. RESULTS: The prevalence ratio of inadequate work ability was 43.42%. Manual patient handling (PR 1.375, 95% CI 1.038-1.821), bachelor education (PR 2.150, 95% CI 1.272-3.632), less than bachelor education (PR 2.166, 95% CI 1.218-3.855), seniority (PR 1.049, 95% CI 1.024-1.086), poor sleep quality (PR 1.425, 95% CI 1.13-1.796) and presence of low back pain (PR 2.003, 95% CI 1.314-3.052) were all positively associated with an inadequate work ability.


Asunto(s)
Dolor de la Región Lumbar , Movimiento y Levantamiento de Pacientes , Enfermedades Profesionales , Brasil/epidemiología , Estudios Transversales , Hospitales , Humanos , Elevación/efectos adversos , Dolor de la Región Lumbar/epidemiología , Enfermedades Profesionales/epidemiología , Evaluación de Capacidad de Trabajo
15.
Acta bioquím. clín. latinoam ; Acta bioquím. clín. latinoam;56(2): 139-148, abr. 2022. graf
Artículo en Español | LILACS, BINACIS | ID: biblio-1402951

RESUMEN

Resumen La ferritina es una proteína de gran tamaño que se encuentra fisiológicamente en el líquido cefalorraquídeo (LCR) en concentraciones de 2-10 ng/mL. Su elevación puede utilizarse como biomarcador en distintas condiciones patológicas. El procedimiento de validación tradicional para la medición en LCR no puede ser utilizado debido a la ausencia de controles y calibradores comerciales para esta matriz. El objetivo de este trabajo fue llevar a cabo una validación analítica de ferritina en LCR. Se realizaron ensayos de estimación de precisión y veracidad mediante el protocolo EP15-A3, linealidad por el protocolo EP6-A (ambos de la guía de la CLSI), recuperación, estabilidad, contaminación por arrastre, interferencia por hemólisis y bilirrubina y límite de detección (LoD). La ferritina en LCR en el autoanalizador DxI 800 de Beckman Coulter tuvo una performance intra e interensayo <3,7%, el ensayo denota linealidad en el intervalo de 2,1-547 ng/mL; se estableció estabilidad por un período de 5 días y la recuperación resultó ser aceptable. No se evidenció efecto de contaminación por arrastre ni interferencia por hemólisis hasta un rango entre 300-500 mg/dL de hemoglobina, ni interferencia por bilirrubina hasta una concentración de 16,0 mg/dL de bilirrubina total. El LoD fue de 0,4 ng/mL. Por medio de los ensayos realizados se logró validar la ferritina en LCR a partir de la utilización de pools de muestras, lo que pudo garantizar la confiabilidad y exactitud del método analítico.


Abstract Ferritin is a large protein physiologically present in the cerebrospinal fluid (CSF) in concentrations of 2-10 ng/mL. Its elevation can be used as a biomarker in several pathological conditions. The traditional validation procedure for measurement in CSF cannot be used due to the absence of commercial controls and calibrators for this matrix. The objective of the present study was to perform CSF ferritin analytical validation. Assays such as precision and accuracy estimation through the EP15-A3 protocol, linearity according to the EP6-A protocol (both from the CLSI guidelines), recovery, stability, carry-over, hemolysis and bilirubin interference and limit of detection (LoD) were conducted. Serum samples with different concentrations of ferritin were added to aliquots of a normal CSF pool. CSF ferritin in the Beckman Coulter DxI 800 had a <3.7% intra and inter-assay performance, the assay shows linearity in the 2.1 -547 ng/mL interval, stability was established for a 5-day period and the recovery was acceptable. There was neither carry-over effect or hemolysis interference up to a range of 300-500 mg/dL of hemoglobin, nor interference by bilirubin up to 16.0 mg/dL of total bilirubin. The LoD was 0.4 ng/mL. By means of the performed assays, CSF ferritin was validated by using sample pools, thereby ensuring the reliability and accuracy of the analytical method.


Resumo A ferritina é uma grande proteína fisiologicamente encontrada no líquido cefalorraquidiano (LCR) em concentrações de 2 a 10 ng/mL. Sua elevação pode ser usada como biomarcador em diferentes condições patológicas. O procedimento de validação tradicional para medição no LCR não pode ser usado devido à ausência de controles e calibradores comerciais para esta matriz. O objetivo deste estudo foi realizar uma validação analítica da ferritina no LCR. Foram realizados estudos de precisão e veracidade utilizando o protocolo EP15-A3, linearidade pelo protocolo EP6-A (ambos das diretrizes do CLSI), recuperação, estabilidade, contaminação transportada, interferência de hemólise e bilirrubina e limite de detecção (LoD). A ferritina no LCR no DxI 800 da Beckman Coulter teve um desempenho intra e inter-ensaio <3,7%, o ensaio denota linearidade na faixa de 2,1-547 ng/mL, a estabilidade foi estabelecida em um período de 5 dias e a recuperação foi considerado aceitável. Nenhum efeito de transporte ou interferência por hemólise foi evidenciado até um intervalo entre 300-500 mg/dL de hemoglobina, nem interferência pela bilirrubina até uma concentração de 16,0 mg/dL de bilirrubina total. O LoD foi de 0,4 ng/mL. Através dos testes realizados, a ferritina no LCR foi validada, com base no uso de pool de amostras, o que poderia garantir a confiabilidade e a acurácia do método analítico.


Asunto(s)
Líquido Cefalorraquídeo , Ferritinas , Bilirrubina , Hemoglobinas , Proteínas , Elevación , Ensayo , Suero , Eficiencia , Contaminación Ambiental , Hemólisis , Métodos
16.
Braz J Phys Ther ; 26(1): 100385, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35063698

RESUMEN

BACKGROUND: A common misconception about low back pain (LBP) is that the spine is weak and that lumbar flexion should be avoided. Because the beliefs of health-care professionals (HCPs) influence patients, it is important to understand the attitudes of health care professionals towards LBP and lifting. OBJECTIVES: To assess and compare the perceptions of different categories of HCPs regarding the safety of specific movement strategies used to lift a light load, and their beliefs regarding back pain. The secondary aim was to determine whether certain factors influenced the beliefs of HCPs. METHODS: Data were collected via an electronic survey. Student and qualified physical therapists (PTs), medical students, and general practitioner (GP) trainees were included. The questionnaire included eight photographs, depicting eight different strategies to lift a light load. Respondents were requested to select the strategy(s) they considered as "unsafe" to use for asymptomatic people with a previous history of LBP and people with chronic LBP. Beliefs and attitudes towards LBP were evaluated using the Back Pain Attitudes Questionnaire (Back-PAQ). RESULTS: Questionnaires from 1005 participants were included. Seventy percent of qualified PTs considered none of the strategies as harmful (versus 32% of PT students, 9% of GP trainees and 1% of medical students). Qualified PTs had higher Back-PAQ scores (mean ± SD: 13.6 ± 5.5) than PT students (8.7 ± 5.7), GP trainees (5.9 ± 5.9) and medical students (4.1 ± 5.2), indicating less misconceptions regarding LBP. Having LBP negatively influenced beliefs while taking a pain education course positively influenced beliefs. CONCLUSION: Misconceptions regarding LBP and the harmfulness of lifting a light load with a rounded back remain common among HCPs, particularly medical doctors.


Asunto(s)
Dolor de la Región Lumbar , Fisioterapeutas , Dolor de Espalda , Humanos , Elevación , Encuestas y Cuestionarios
17.
Aesthet Surg J ; 42(3): NP144-NP150, 2022 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-34373897

RESUMEN

BACKGROUND: Facial nerve injury after facelift is rare; hence, its treatment is poorly established. Botulinum toxin type A (BTXA) can be employed to resolve the asymmetry. To our knowledge, there is no protocol in the literature about the best timing for this treatment, injection sites, or recommended dose. OBJECTIVES: The authors sought to propose a protocol to guide the management of asymmetries post-facelift. METHODS: Fifteen patients with post-rhytidectomy facial palsies were treated in the non-paralyzed side with BTXA. After analysis of the smile deviation vectors, it is possible to identify the muscles that should be treated. The dose varied from 1 to 2 volume-unit per point. Patients were examined after 15 days for outcomes evaluation and touch-up if needed. Patients were re-treated after 5 to 6 months in case of asymmetry recurrence. RESULTS: Symmetry was achieved in all cases. Six patients had definitive nerve lesions and required treatment every 6 months after the first session. Five patients had lesions affecting the upper third of the face; 4 of them were definitive nerve lesions. Two of the 4 patients who were treated less than 2 weeks after surgery recovered early from the post-facelift paralysis and developed reversed asymmetry due to the BTXA. In 7 patients, the post-facelift asymmetry was due to neuropraxis: the recovery from the nerve injury and BTXA treatment occurred symmetrically on both sides of the face in the following months after 1 single session. CONCLUSIONS: Asymmetries post-facelift were successfully managed with the proposed protocol. The best time for injection was 2 to 4 weeks after surgery.


Asunto(s)
Toxinas Botulínicas Tipo A , Parálisis Facial , Fármacos Neuromusculares , Ritidoplastia , Cara , Expresión Facial , Músculos Faciales , Parálisis Facial/tratamiento farmacológico , Parálisis Facial/etiología , Humanos , Fármacos Neuromusculares/uso terapéutico
18.
Scand J Pain ; 22(2): 336-347, 2022 04 26.
Artículo en Inglés | MEDLINE | ID: mdl-34821139

RESUMEN

OBJECTIVES: Previous studies in a high-income country have demonstrated that people with and without low back pain (LBP) have an implicit bias that bending and lifting with a flexed lumbar spine is dangerous. These studies present two key limitations: use of a single group per study; people who recovered from back pain were not studied. Our aims were to evaluate: implicit biases between back posture and safety related to bending and lifting in people who are pain-free, have a history of LBP or have current LBP in a middle-income country, and to explore correlations between implicit and explicit measures within groups. METHODS: Exploratory cross-sectional study including 174 participants (63 pain-free, 57 with history of LBP and 54 with current LBP). Implicit biases between back posture and safety related to bending and lifting were assessed with the Implicit Association Test (IAT). Participants completed paper-based (Bending Safety Belief [BSB]) and online questionnaires (Tampa Scale of Kinesiophobia; Back Pain Attitudes Questionnaire). RESULTS: Participants displayed significant implicit bias between images of round-back bending and lifting and words representing "danger" (IATD-SCORE: Pain-free group: 0.56 (IQR=0.31-0.91; 95% CI [0.47, 0.68]); history of LBP group: 0.57 (IQR=0.34-0.84; 95% CI [0.47, 0.67]); current LBP group: 0.56 (IQR=0.24-0.80; 95% CI [0.39, 0.64])). Explicit measures revealed participants hold unhelpful beliefs about the back, perceiving round-back bending and lifting as dangerous (BSBthermometer: Pain-free group: 8 (IQR=7-10; 95% CI [7.5, 8.5]); history of LBP group: 8 (IQR=7-10; 95% CI [7.5, 9.0]); current LBP group: 8.5 (IQR=6.75-10; [7.5, 9.0])). There was no correlation between implicit and explicit measures within the groups. CONCLUSIONS: In a middle-income country, people with and without LBP, and those who recovered from LBP have an implicit bias that round-back bending and lifting is dangerous.


Asunto(s)
Elevación , Dolor de la Región Lumbar , Sesgo Implícito , Estudios Transversales , Humanos , Elevación/efectos adversos , Postura
19.
Braz. dent. sci ; 25(2): 1-13, 2022. tab
Artículo en Inglés | LILACS, BBO - Odontología | ID: biblio-1368754

RESUMEN

Background: The debonding of crowns and ceramic veneers with laser is already a reality in the clinic. It presents benefits in comparison to traditional removal with the use of a rotating instrument; however, there is still no consolidated protocol and many professionals use it without the necessary scientific basis. Objective: The aim of this work was to perform a literature review on the debonding of indirect ceramic restorations using the Er:YAG and Er,Cr:YSGG lasers to provide clinical professionals and the scientific community a careful analysis, and also to guiding the use of irradiation at laser for such procedures. Methods: Bibliographic searches were performed in the following databases: Pubmed, Web of Science and Google Scholar. According to the inclusion criteria adopted, twenty-seven clinical and in vitro studies were selected in the period from 2007 to 2021. In the present work, the composition of the crowns and facets, as well as the types of cement and the laser irradiation protocols adopted in the selected studies is detailed. Conclusions: It was concluded that the use of erbium lasers for debonding of indirect ceramic restorations proved to be more selective and conservative when compared to removal with a rotary diamond instrument. Furthermore, it was shown to be in more efficient in debonding different types of ceramics. However, there are great variations in the "debonding" protocols, which emphasizes the need for further studies that seek to standardize the irradiation protocols considering the different clinical situations (AU)


Antecedentes: A remoção de coroas e facetas cerâmicas com laser já é uma realidade clínica e apresenta benefícios em relação à remoção tradicional com uso de instrumento rotatório diamantado; entretanto, ainda não existe um protocolo consolidado e muitos profissionais o utilizam sem o embasamento científico necessário. Objetivos: O objetivo deste trabalho foi realizar uma revisão da literatura sobre a remoção de restaurações cerâmicas indiretas utilizando os lasers Er:YAG e Er,Cr:YSGG para fornecer uma análise cuidadosa aos profissionais clínicos e à comunidade científica, além de orientar o uso da irradiação a laser para tal aplicação. Métodos: As buscas bibliográficas foram realizadas, nas seguintes bases de dados: Pubmed, Web of Science e Google Scholar. De acordo com os critérios de inclusão adotados, foram selecionados vinte e sete estudos clínicos e in vitro no período de 2007 a 2021. No presente trabalho, a composição das coroas e facetas, bem como os tipos de cimento e os protocolos de irradiação laser adotados nos estudos selecionados foram detalhados. Conclusão: Concluiu-se que o uso dos lasers de érbio para remoção de restaurações cerâmicas indiretas mostrou-se mais seletivo e conservador quando comparado à remoção com instrumento rotatório diamantado, além de ser eficiente na remoção de diferentes tipos de cerâmicas. No entanto, existem grandes variações nos protocolos de "debonding", o que enfatiza a necessidade de novos estudos que busquem uma padronização dos protocolos de irradiação considerando as diferentes situações clínicas.(AU)


Asunto(s)
Elevación , Coronas , Coronas con Frente Estético , Láseres de Estado Sólido
20.
Braz. dent. sci ; 25(3): 1-6, 2022. ilus
Artículo en Inglés | LILACS, BBO - Odontología | ID: biblio-1391034

RESUMEN

Hereby, we objectively assessed the outcomes of a facial-lifting procedure with Botulinum toxin type A (BoNT-A) using a 3D stereophotogrammetry quantification (3D-SQ). A 46-year-old female patient received a full face BoNT-A treatment in a total dose of 180 Speywood Units (sU). Frontal, lateral and oblique photographs were taken before and 20 days after treatment, at rest and during mimic movements. Also, a facial scanning was performed before and 20 days after BoNT-A injections. The results were analyzed using a 3D-SQ software. The photographs showed a decrease in expression lines and dynamic wrinkles. In addition, a better-defined jawline and volume gain in the midface area with improvement of the profile appearance, due to the reduction of the sagging skin under the chin, was observed. The 3D-SQ showed volume gains of 1.17 ml on the right and of 1.59 ml on the left cheekbone areas, due to the cranially soft-tissue repositioning. In addition, a decrease in the volume of melomental folds areas (0.27ml on the right and 0.41 ml on the left side) was reported, compatible to the above-mentioned volume gain. Measurements considering cephalometric points showed a decrease in the total facial height (distance from Trichion to Mental points), suggesting a soft tissue dislocation in an upward direction. Finally, this case report showed quantitative results that can evidence the role of BoNT-A in facial- lifting procedures. These results reinforce the importance of using a 3D-SQ to assess the outcomes of BoNT-A and, probably, other aesthetic procedures.(AU)


No presente estudo avaliamos objetivamente os resultados de um procedimento de lifting facial com toxina botulínica tipo A (BoNT-A) usando uma quantificação de estereofotogrametria 3D (3D-SQ). Uma paciente do sexo feminino de 46 anos recebeu um tratamento facial completo com BoNT-A em uma dose total de 180 unidades Speywood (sU). Fotografias frontais, laterais e oblíquas foram tomadas antes e 20 dias após o tratamento, em repouso e durante os movimentos mímicos. Além disso, um escaneamento facial foi realizado antes e 20 dias após as injeções de BoNT-A. Os resultados foram analisados por meio de um software 3D-SQ. As fotografias mostraram uma diminuição das linhas de expressão e rugas dinâmicas. Além disso, observou-se um maxilar mais bem definido e ganho de volume na região média da face com melhora da aparência do perfil, devido à redução da flacidez da pele sob o queixo. O 3D-SQ apresentou ganho de volume de 1,17 ml à direita e 1,59 ml à esquerda, devido ao reposicionamento do tecido mole. Além disso, foi relatada diminuição do volume das áreas do sulco mentual (0,27 ml à direita e 0,41ml à esquerda), compatível com o ganho de volume acima citado. As medidas considerando os pontos cefalométricos mostraram uma diminuição da altura facial total (distância dos pontos Triquion ao Mentual), sugerindo um deslocamento superior dos tecidos moles. Em conclusão, este relato de caso mostrou resultados quantitativos que podem evidenciar o papel da BoNT-A em procedimentos de lifting facial. Esses resultados reforçam a importância da 3D-SQ para avaliar objetivamente os resultados de harmonização orofacial com BoNT-A e outros materiais. (AU)


Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Fotogrametría , Elevación , Toxinas Botulínicas Tipo A , Fotografía Dental , Músculos del Cuello
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