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Plant Cell Physiol ; 59(11): 2188-2203, 2018 Nov 01.
Article in English | MEDLINE | ID: mdl-30239816

ABSTRACT

Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.


Subject(s)
Carrier Proteins/metabolism , Plant Proteins/metabolism , Solanum lycopersicum/metabolism , alpha-Tocopherol/metabolism , Chloroplasts/metabolism , Endoplasmic Reticulum/metabolism , Gene Knockdown Techniques , Lipid Metabolism , Solanum lycopersicum/genetics , Phylogeny , Plant Proteins/genetics , Plastids/metabolism
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