Arsenic uptake by Agrostis capillaris, as related to its genotypic diversity in the area of historical ore mining and processing.

Common bentgrass Agrostis capillaris L. is known as tolerant to toxic elements. A hypothesis was examined that its ecotypes growing in historically polluted sites show a limited arsenic uptake and have genetic features that distinguish them from commercially available cultivars. The study was conducted in Zloty Stok, a historical area of arsenic mining. Additionally, two commercial cultivars were grown in pots with arsenic-rich soils. Based on arsenic concentrations in plant roots and shoots, bioconcentration and translocation factors BCF and TF were calculated. Commercial cultivars indicated many times higher BCF shoots and TF values compared to field plants. DNA analysis of leaf blades showed a clear distinction between the plants growing in some sites and patches in the field, and also a gene overlap between the plants in the field and commercial forms. The research did not allow for identification of ecotypes with exceptionally limited arsenic uptake. Moreover, there were no significant differences between the genotypic characteristics of plants growing in polluted sites and those poorly tolerant grown from commercially available seeds. Apparently, other factors, and not genetically determined features, are responsible for A. capillaris tolerance to arsenic in Zloty Stok.

Arsenic toxicity to earthworms in soils of historical As mining sites: an assessment based on various endpoints and chemical extractions.

Eisenia fetida is an earthworm species often used to assess the toxicity of contaminants in soils. Several studies indicated that its response can be unpredictable because it depends both on total concentrations of contaminants and also on their forms that differ in susceptibility to be released from soil solid phase. The issue is complex because two various uptake routes are concurrently involved, dermal and ingestion in guts, where the bioavailability of contaminants can considerably change. The aim of this study was to analyze the toxicity of arsenic (As) in various strongly contaminated meadow and forest soils, representative for former As mining and processing area, to earthworms E. fetida and its accumulation in their bodies. An attempt was made to find relationships between the response of earthworms and chemical extractability of As. In the bioassay, carried out according to the standard ISO protocol, different endpoints were applied: earthworm survival, fecundity measured by the numbers of juveniles and cocoons, earthworm weight and As accumulation in the bodies. The results proved that E. fetida can tolerate extremely high total As concentrations in soils, such as 8000 mg/kg, however, the individual endpoints were not correlated and showed different patterns. The most sensitive one was the number of juveniles. No particular soil factor was identified that would indicate an exceptionally high As susceptibility to the release from one of soils, however, we have demonstrated that the sum of non-specifically and specifically bound As (i.e. fractions F1 + F2 in sequential extraction according to Wenzel) could be a good chemical indicator of arsenic toxicity to soil invertebrates.

Back to Locality? Demand Potential Analysis for Short Food Supply Chains.

The main purpose of the article is an attempt to estimate the demand for products traded within short food supply chains in Poland. The survey was conducted in autumn 2021 in the Kamienna Góra county, where the first business incubator in Poland, addressed to farmers and food producers, initiated and supported by the local government, is located. The Computer-Assisted Web Interview (CAWI) method constituted the basis for the process of collecting research material. The channel for reaching respondents was the LIBRUS application and local social media. Responses were mainly given by women, people with incomes in the range of PLN 1000-3000 per person, those aged 30-50, and those with a university education. The research results showed a high level of potential demand for local agri-food products, which should encourage farmers to switch from long to short supply chain models. The persisting low awareness regarding the existence of alternative distribution networks for local products, which primarily requires increased activities in the field of territorial marketing that promote local agri-food products among the residents of municipalities constitutes, from the perspective of consumers, a barrier to the development of short food supply chains.

Arsenic Uptake by Two Tolerant Grass Species: Holcus lanatus and Agrostis capillaris Growing in Soils Contaminated by Historical Mining.

The study focused on two grass species Holcus lanatus and Agrostis capillaris abundant in the sites of former As mining and processing in the Sudetes. Arsenic uptake from soils was examined to assess a risk associated with its accumulation in grass shoots and to check its dependence on soil fertilization. The research involved a field study and greenhouse experiment. In the field study, soil and plant samples were collected from 33 sites with 72-98,400 mg/kg total soil As. Arsenic uptake by grasses differed widely. Both species indicated a strategy typical for eliminators, although As concentrations in more than 50% of the shoot samples exceeded 4 mg/kg, a maximum permissible value for fodder. In the greenhouse experiment, commercial cultivars of both species were grown in five soils containing 394-19,600 mg/kg, untreated and fertilized. All seedlings died in the soil with highest total As, and considerable phytotoxicity was observed in other soils, particularly in nonfertilized ones. Fertilization resulted in the improvement of plant growth and reduction of As uptake except for Agrostis capillaris fertilized with manure. Further research should focus on identifying tolerant genotypes growing in extremely enriched sites and analysis of factors that will efficiently reduce As phytoaccumulation.

Accumulation of Arsenic by Plants Growing in the Sites Strongly Contaminated by Historical Mining in the Sudetes Region of Poland.

The uptake of As by various plants growing in highly enriched sites was examined in order to identify potential As accumulators and to assess the risk associated with As presence in plant shoots. Representative samples of 13 plant species, together with soil samples, were collected from various sites affected by historical As mining: mine and slag dumps, tailings and contaminated soils with As concentrations in a range 72-193,000 mg/kg. Potentially and actually soluble As forms, extracted with 0.43 M HNO3 and, 1M NH4NO3 were examined in relation to As concentrations in plant roots and shoots. The latter differed strongly among the species and within them and were in the ranges 2.3-9400 mg/kg and 0.5-509 mg/kg, respectively. The majority (over 66%) of plant samples had As shoot concentrations above 4 mg/kg, an upper safe limit for animal fodder. The uptake of As by plants correlated well with total and extractable soil As, though As concentrations in plants could not be predicted based on soil parameters. Equisetum spp. and C. epigejos indicated a particularly strong accumulation of As in shoots, while A. capillaris, and H. lanatus showed a limited As root-to-shoot transfer, apparently associated with species-related tolerance to As.

Arsenic accumulation by red fescue (Festuca rubra) growing in mine affected soils - Findings from the field and greenhouse studies.

Soils strongly enriched in arsenic in historical mining sites pose the environmental risk. Phytostabilization is a reasonable method for their remediation. A suitability of red fescue (Festuca rubra L.) for this purpose was examined. Plant and soil material was collected from four study objects: mine dumps in Zloty Stok and Czarnów and two areas formerly flooded by tailings. Total As in soils ranged 72-48900 mg/kg, while the shoots and roots of red fescue contained 1.5-65.5 and 2.3-824 mg/kg As, respectively. Bioaccumulation BAF and translocation TF factors were typical for excluders, however, in most cases, As in shoots exceeded 4 mg/kg, an EU threshold for As in fodder. A greenhouse experiment, that involved treatment with mineral fertilizers, manure, and forest litter, was performed to closer examine the factors governing As uptake by red fescue. A stress-resistant cultivar Leo-Pol was used as a test plant. Grass shoots were harvested after 6 and 12 weeks. Manure treatment increased strongly As extractability but did not increase As uptake by plants. Though, As concentrations in plants were in the pot experiment by manifold higher than those in the field. Particularly high (66.5-1580 mg/kg) was As in the second shoot harvest. Differences between the field and greenhouse data indicate that the populations of red fescue, that develop in As rich sites, are specifically As-tolerant. Possible mechanisms of tolerance are discussed. The conclusion is that the commercial cultivar, despite declared stress-resistance, cannot be used for phytostabilization of barren As-rich soils.

Ecotoxicity of pore water in soils developed on historical arsenic mine dumps: The effects of forest litter.

Arsenic release from dump soils in historical mining sites poses the environmental risk. Decomposing forest litter can affect mobilization of As and other toxic elements, change their speciation in pore water and influence the toxicity to biota. This study examined the chemistry and ecotoxicity of pore water acquired from four soils that developed on the dumps in former As mining sites, in the presence and absence of forest litter collected from beech and spruce stands. Soils contained 1540-19600 mg/kg of As. Pore water was collected after 2, 7, 21 and 90 days of incubation, using MacroRhizon suction samplers. Its chemical analysis involved determination of pH, the concentrations of As, Cu and Pb (the elements with high enrichment factor Igeo>3), as well as metals considered most mobile: Cd, Zn and Mn. Ecotoxicity of pore water was examined in three bioassays: Microtox, MARA and Phytotox with Sinapis alba as test plant. The release of As, unlike heavy metals, was particularly intensive from the soils with neutral and alkaline pH. The concentrations of toxic elements in pore water were in broad ranges, up to dozens mg/L. The results of Phytotox had a poor precision, but their means correlated well with As concentrations in pore water, which indicates that As made a crucial factor of phytotoxicity. The outcomes of Microtox bioassay indicated poorer relationships between As concentrations and toxicity, and other factors contributed to ecotoxicity at very low and very high As concentrations. The highest toxicity was recorded from the soils treated with forest litter. MARA turned out to be not sensitive enough to give reproducible results in experimental conditions. The PCA analysis confirmed that the growth of microbes in MARA bioassay was poorly dependent on As and metals in pore water except for a yeast Pichia anomala (No 11). The results let us conclude that the bioassays Phytotox and Microtox can provide useful information on ecotoxicity of pore water in soils that develop on As-rich dumps whereas applicability of MARA in those conditions proved limited.

Some adverse effects of soil amendment with organic Materials-The case of soils polluted by copper industry phytostabilized with red fescue.

The study was aimed to examine the effects of soil amendment with organic waste materials on the growth of red fescue and the uptake of Cu and Zn by this grass, in view of its potential usage for phytostabilization of Cu-polluted soils. Five soils, containing 301-5180 mg/kg Cu, were collected from the surroundings of copper smelter Legnica, and amended with lignite (LG) and limed sewage sludge (SS). Plant growth and the concentrations of Cu and Zn in the shoots and roots of grass were measured in a pot experiment and related to the results of Pytotoxkit and Microtox® tests performed on soil solution. The effects of soil amendment with LG and SS differed greatly, and depended on soil properties. In some cases, the application of alkaline SS resulted in dramatic increase of Cu phytotoxicity and its enhanced uptake by plants, while application of LG to slightly acidic soil caused increased accumulation of Zn in plants, particularly in their roots. The study confirmed good suitability of red fescue for phytostabilization of Cu-contaminated soils except for those extremely polluted. Organic amendments to be used for metal immobilization should be thoroughly examined prior to application.