Shrimp hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation.

Conjugation to a carrier protein is essential to give rise to the antigenicity of hapten. Three carrier proteins e.g. KLH (Keyhole Limpet hemocyanin), BSA (bovine serum albumin), and OVA (Ovalbumin) were used mostly. KLH is advantageous to the others, majorly owing to its strong immunogenicity and limited usage in other biological assays. However, the cost of obtaining Keyhole Limpet is high and the solubility of KLH is not as well as the other carriers, especially after hapten conjugation. Here, we extracted the shrimp hemocyanin (SHC) from Litopenaeus vannamei (L. vannamei), which is a commonly sea product worldwide. The high pure SHC could be acquired by two-step purification, with a production yield of > 1 g proteins (98% pure) per 1 kg shrimp. Compared to KLH, the peptide-SHC conjugates exhibit higher solubility after hapten conjugation. Meanwhile, compared with KLH, SHC induces comparable antibody production efficiency in mammals, with or without conjugation. Furthermore, rabbit polyclonal antibodies or mouse monoclonal antibodies were generated by immunizing SHC-peptide conjugates, and the subsequent antibodies were confirmed to be used in western blot, immunofluorescence and immunohistochemistry. Therefore, we demonstrated that SHC may be used as a substitute for KLH in future antibody and vaccine development.

Data Clothing and BigBarChart: Designing Physical Data Reports on Indoor Pollutants for Individuals and Communities.

In response to participant preferences and new ethics guidelines, researchers are increasingly sharing data with health study participants, including data on their own household chemical exposures. Data physicalization may be a useful tool for these communications, because it is thought to be accessible to a general audience and emotionally engaged. However, there are limited studies of data physicalization in the wild with diverse communities. Our application of this method in the Green Housing Study is an early example of using data physicalization in environmental health report-back. We gathered feedback through community meetings, prototype testing, and semistructured interviews, leading to the development of data t-shirts and other garments and person-sized bar charts. We found that participants were enthusiastic about data physicalizations, it connected them to their previous experience, and they had varying desires to share their data. Our findings suggest that researchers can enhance environmental communications by further developing the human experience of physicalizations and engaging diverse communities.