ABSTRACT
Background: Feline Injection Site-Associated Sarcoma (FISS) is a mesenchymal neoplasia of aggressive behavior thatdevelops in sites where vaccine or drugs were administered. FISS is clinically characterized by the appearance of asolitary firm nodule or a diffuse mass, adhered to tissues, in regions associated to vaccine or drug applications. Despitelow prevalence, tumor recurrence rates can reach 80%. FISS present more aggressive histological characteristics whencompared to sarcomas not associated to injection sites. The aim of this paper is to contribute towards the understandingof the biological behavior of FISS.Materials, Methods & Results: Sixteen samples of FISS were analyzed. Fibrosarcomas were the most frequent histological subtype (62.5%). Malignant peripheral nerve sheath tumor was diagnosed in 18.75% cases. Ten (62.5%) FISS wereclassified as grade II; 4/16 (25%) grade I, and 2/16 (12.5%) grade III. Cox-2 overexpression occurred in 3/16 (18.75%)samples, with positive correlation between Cox-2 expression and cellularity (r = 0.696, P = 0.003). Mitotic index lowerthan 9 events was found in 11/16 (68.7%) samples and between 10 and 19 mitotic events in 5/16 (31.3%) cases. MeanKi-67 expression was 2.39 ± 2.48%. FISS characterized as fibrosarcomas presented longer overall survival (median 545days) than other histological subtypes (median 130.5 days) [P = 0.01].Discussion: Patients with FISS generally present with larger nodules than those with sarcomas not associated to injections,suggesting a challenge for pet owners to note subcutaneous tumors in the interscapular region, in addition to the aggressivebiological behavior of FISS. The influence of size on prognosis remains controversial. An association between histologicgrade and the development of metastasis has been observed, with patients with grade III FISS associated with an increasein the metastatic rate. The present study did not find a correlation between overall survival...(AU)
Subject(s)
Animals , Cats , Sarcoma/veterinary , Injections/adverse effects , Cats , Immunohistochemistry/veterinaryABSTRACT
Background: Feline Injection Site-Associated Sarcoma (FISS) is a mesenchymal neoplasia of aggressive behavior thatdevelops in sites where vaccine or drugs were administered. FISS is clinically characterized by the appearance of asolitary firm nodule or a diffuse mass, adhered to tissues, in regions associated to vaccine or drug applications. Despitelow prevalence, tumor recurrence rates can reach 80%. FISS present more aggressive histological characteristics whencompared to sarcomas not associated to injection sites. The aim of this paper is to contribute towards the understandingof the biological behavior of FISS.Materials, Methods & Results: Sixteen samples of FISS were analyzed. Fibrosarcomas were the most frequent histological subtype (62.5%). Malignant peripheral nerve sheath tumor was diagnosed in 18.75% cases. Ten (62.5%) FISS wereclassified as grade II; 4/16 (25%) grade I, and 2/16 (12.5%) grade III. Cox-2 overexpression occurred in 3/16 (18.75%)samples, with positive correlation between Cox-2 expression and cellularity (r = 0.696, P = 0.003). Mitotic index lowerthan 9 events was found in 11/16 (68.7%) samples and between 10 and 19 mitotic events in 5/16 (31.3%) cases. MeanKi-67 expression was 2.39 ± 2.48%. FISS characterized as fibrosarcomas presented longer overall survival (median 545days) than other histological subtypes (median 130.5 days) [P = 0.01].Discussion: Patients with FISS generally present with larger nodules than those with sarcomas not associated to injections,suggesting a challenge for pet owners to note subcutaneous tumors in the interscapular region, in addition to the aggressivebiological behavior of FISS. The influence of size on prognosis remains controversial. An association between histologicgrade and the development of metastasis has been observed, with patients with grade III FISS associated with an increasein the metastatic rate. The present study did not find a correlation between overall survival...
Subject(s)
Animals , Cats , Cats , Injections/adverse effects , Sarcoma/veterinary , Immunohistochemistry/veterinaryABSTRACT
Parvoviral enteritis is a common viral infection in dogs and is associated with many clinical and hematological changes. Bacterial translocation is a common complication and may result in sepsis. The objective of this study is to determine the presence of clinical and hematological factors associated with the risk of death in puppies with naturally occurring parvoviral enteritis and sepsis. Twenty-four dogs with parvoviral enteritis confirmed by chromatographic immunoassay during the clinical routine of a university veterinary hospital were selected. At admission and every 24 hours until the third day of hospitalization or until death, venous blood samples were collected for complete blood count, renal and hepatic biochemistry, and lactate and magnesium measurement; arterial blood samples were collected for gas analysis. Sodium, potassium, and ionized calcium were also analyzed, and a complete physical examination was performed. The factors associated with mortality were evaluated by Cox univariate analysis at a level of significance of 5%. The increase in urea and heart rate was associated with an increase in the risk of death. In contrast, an increase in total leukocytes, lymphocytes, monocytes, partial pressure of oxygen, base deficit, bicarbonate ion, and oxygen saturation were associated with a reduction in the risk of death.(AU)
A enterite por Parvovírus é uma infecção viral comum em cães e é associada a diversas alterações clínicas e hematológicas. A translocação bacteriana é uma complicação comum e pode resultar em sepse. O objetivo do estudo foi determinar a presença de fatores clínicos e hematológicos associados ao risco de óbito em filhotes com parvovirose de ocorrência natural e sepse. Vinte e quatro cães com parvovirose, confirmada através de imunoensaio cromatográfico, foram selecionados a partir da rotina clínica do Hospital Veterinário. À admissão e a cada 24 horas até o terceiro dia de hospitalização ou até óbito, amostras de sangue venoso foram coletadas para realização de hemograma, bioquímica renal e hepática, mensuração de lactato e magnésio; amostras de sangue arterial foram coletadas para gasometria. Foram analisados também sódio, potássio e cálcio ionizado, bem como foi realizado o exame físico completo. Os fatores associados à mortalidade foram avaliados através de análise univariada de Cox com um nível de significância de 5%. O aumento da concentração de ureia e da frequência cardíaca foram associados à elevação do risco de óbito. Em contraste, o aumento nos valores de leucócitos totais, linfócitos, monócitos, pressão parcial de oxigênio, déficit de base, íon bicarbonato e saturação de oxigênio foram associados à redução do risco de óbito.(AU)
Subject(s)
Animals , Child , Dogs , Parvovirus, Canine/pathogenicity , Enteritis/diagnosis , Enteritis/veterinary , Enteritis/mortality , Sepsis/veterinary , Sepsis/complications , Bacterial TranslocationABSTRACT
Parvoviral enteritis is a common viral infection in dogs and is associated with many clinical and hematological changes. Bacterial translocation is a common complication and may result in sepsis. The objective of this study is to determine the presence of clinical and hematological factors associated with the risk of death in puppies with naturally occurring parvoviral enteritis and sepsis. Twenty-four dogs with parvoviral enteritis confirmed by chromatographic immunoassay during the clinical routine of a university veterinary hospital were selected. At admission and every 24 hours until the third day of hospitalization or until death, venous blood samples were collected for complete blood count, renal and hepatic biochemistry, and lactate and magnesium measurement; arterial blood samples were collected for gas analysis. Sodium, potassium, and ionized calcium were also analyzed, and a complete physical examination was performed. The factors associated with mortality were evaluated by Cox univariate analysis at a level of significance of 5%. The increase in urea and heart rate was associated with an increase in the risk of death. In contrast, an increase in total leukocytes, lymphocytes, monocytes, partial pressure of oxygen, base deficit, bicarbonate ion, and oxygen saturation were associated with a reduction in the risk of death.
A enterite por Parvovírus é uma infecção viral comum em cães e é associada a diversas alterações clínicas e hematológicas. A translocação bacteriana é uma complicação comum e pode resultar em sepse. O objetivo do estudo foi determinar a presença de fatores clínicos e hematológicos associados ao risco de óbito em filhotes com parvovirose de ocorrência natural e sepse. Vinte e quatro cães com parvovirose, confirmada através de imunoensaio cromatográfico, foram selecionados a partir da rotina clínica do Hospital Veterinário. À admissão e a cada 24 horas até o terceiro dia de hospitalização ou até óbito, amostras de sangue venoso foram coletadas para realização de hemograma, bioquímica renal e hepática, mensuração de lactato e magnésio; amostras de sangue arterial foram coletadas para gasometria. Foram analisados também sódio, potássio e cálcio ionizado, bem como foi realizado o exame físico completo. Os fatores associados à mortalidade foram avaliados através de análise univariada de Cox com um nível de significância de 5%. O aumento da concentração de ureia e da frequência cardíaca foram associados à elevação do risco de óbito. Em contraste, o aumento nos valores de leucócitos totais, linfócitos, monócitos, pressão parcial de oxigênio, déficit de base, íon bicarbonato e saturação de oxigênio foram associados à redução do risco de óbito.
Subject(s)
Animals , Child , Dogs , Enteritis/diagnosis , Enteritis/mortality , Enteritis/veterinary , Parvovirus, Canine/pathogenicity , Sepsis/complications , Sepsis/veterinary , Bacterial TranslocationABSTRACT
Infectious diseases can cause deleterious effects on bird species, leading to population decline and extinction. Haemosporidia can be recognized by their negative effects on host fitness, including reproductive success and immune responses. In captivity, outbreaks of haemosporidian infection have been observed in birds in zoos and aviaries. The endemic Brazilian Atlantic rainforest species Aburria jacutinga is one of the most endangered species in the Cracidae family, and wild populations of this species are currently found mainly in conservation areas in only two Brazilian states. In this study, we aimed to evaluate the effects of avian haemosporidia on hematological and biochemical parameters in two captive populations of A. jacutinga. Forty-two animals were assessed, and the haemosporidian prevalence was similar for males and females. The occurrence of haemosporidian infection in captive A. jacutinga observed in this study was similar to results found in other captive and wild birds in Brazil. We found three different lineages of haemosporidia. Two lineages were identified as Plasmodium sp., one of which was previously detected in Europe and Asia, and the other is a new lineage closely related to P. gallinaceum. A new third lineage was identified as Haemoproteus sp. We found no significant differences in hematological and biochemical values between infected and non-infected birds, and the haemosporidian lineage did not seem to have an impact on the clinical and physiological parameters of A. jacutinga. This is the first report on an evaluation of natural haemosporidian infections diagnosed by microscopic and molecular methods in A. jacutinga by hematology, blood biochemistry, and serum protein values. Determining physiological parameters, occurrence and an estimation of the impact of haemosporidia in endangered avian species may contribute to the management of species rehabilitation and conservation.