ABSTRACT
OBJECTIVE: The objective of these guidelines is to define for women at low obstetric risk modalities that respect the physiology of delivery and guarantee the quality and safety of maternal and newborn care. METHODS: These guidelines were made by a consensus of experts based on an analysis of the scientific literature and the French and international recommendations available on the subject. RESULTS: It is recommended to conduct a complete initial examination of the woman in labor at admission (consensus agreement). The labor will be monitored using a partogram that is a useful traceability tool (consensus agreement). A transvaginal examination may be offered every two to four hours during the first stage of labor and every hour during the second stage of labor or before if the patient requests it, or in case of a warning sign. It is recommended that if anesthesia is required, epidural or spinal anesthesia should be used to prevent bronchial inhalation (grade A). The consumption of clear fluids is permitted throughout labor in patients with a low risk of general anesthesia (grade B). It is recommended to carry out a "low dose" epidural analgesia that respects the experience of delivery (grade A). It is recommended to maintain the epidural analgesia through a woman's self-administration pump (grade A). It is recommended to give the woman the choice of continuous (by cardiotocography) or discontinuous (by cardiotocography or intermittent auscultation) monitoring if the conditions of maternity organization and the permanent availability of staff allow it and, after having informed the woman of the benefits and risks of each technique (consensus agreement). In the active phase of the first stage of labor, the dilation rate is considered abnormal if it is less than 1cm/4h between 5 and 7cm or less than 1cm/2h above 7cm (level of Evidence 2). It is then recommended to propose an amniotomy if the membranes are intact or an oxytocin administration if the membranes are already ruptured, and the uterine contractions considered insufficient (consensus agreement). It is recommended not to start expulsive efforts as soon as complete dilation is identified, but to let the presentation of the fetus drop (grade A). It is recommended to inform the gynecologist-obstetrician in case of nonprogression of the fetus after two hours of complete dilation with sufficient uterine dynamics (consensus agreement). It is recommended not to use abdominal expression (grade B). It is recommended to carry out preventive administration of oxytocin at 5 or 10 IU to prevent PPH after vaginal delivery (grade A). In the case of placental retention, it is recommended to perform a manual removal of the placenta (grade A). In the absence of bleeding, it should be performed 30minutes but not more than 60minutes after delivery (consensus agreement). It is recommended to assess at birth the breathing or screaming, and tone of the newborn to quickly determine if resuscitation is required (consensus agreement). If the parameters are satisfactory (breathing present, screaming frankly, and normal tonicity), it is recommended to propose to the mother that she immediately place the newborn skin-to-skin with her mother if she wishes, with a monitoring protocol (grade B). Delayed cord clamping is recommended beyond the first 30seconds in neonates, not requiring resuscitation (grade C). It is recommended that the first oral dose (2mg) of vitamin K (consensus agreement) be given systematically within two hours of birth. CONCLUSION: These guidelines allow women at low obstetric risk to benefit from a better quality of care and optimal safety conditions while respecting the physiology of delivery.
Subject(s)
Gynecology , Midwifery , Delivery, Obstetric , Female , Humans , Oxytocin , Placenta , PregnancyABSTRACT
Fatty acids of marine origin have been shown to affect blood coagulation in the rat. In an attempt to gain insight into the mechanisms of this phenomenon, we studied the effects of dietary linseed and fish oils on the liver antioxidant status and plasma coagulation parameters in rats on a time-course basis. Dietary enrichment in eicosapentaenoic and docosahexaenoic acids resulted in strong hypocoagulation after only 1 week and a concomitant increase in liver lipid peroxidation and tocopherolquinone content. Enrichment in linolenic acid induced similar increases in lipid peroxidation and tocopherol catabolism but negligible alteration of coagulation. A significant correlation between plasma factor II coagulant activity and liver tocopherolquinone was found in fish oil- but not in linseed oil-fed rats. Although ingestion of tocopherolquinone led to high levels of this compound in the liver, it had only marginal effects on coagulation factors. Thus, it seems unlikely that this vitamin E metabolite could be involved in the lowering of vitamin K-dependent clotting factors through inhibition of gamma-glutamylcarboxylase. Rather, our results indicate that the effects of the n-3 fatty acids of fish oil on vitamin K-dependent coagulation factors are specific and independent of liver tocopherolquinone levels.
Subject(s)
Blood Coagulation Factors/drug effects , Fatty Acids, Omega-3/pharmacology , Vitamin E/analogs & derivatives , Vitamin K/physiology , Animals , Anticoagulants/metabolism , Anticoagulants/pharmacology , Blood Coagulation/drug effects , Blood Coagulation Factors/metabolism , Cholesterol/blood , Dietary Fats, Unsaturated/pharmacology , Fatty Acids/analysis , Fibrinogen/drug effects , Fibrinogen/metabolism , Lipid Peroxidation/drug effects , Lipids/blood , Liver/drug effects , Liver/metabolism , Male , Oxidative Stress , Phospholipids/chemistry , Phospholipids/metabolism , Rats , Rats, Wistar , Thiobarbituric Acid Reactive Substances/metabolism , Triglycerides/blood , Vitamin E/metabolism , Vitamin E/pharmacologyABSTRACT
BACKGROUND: Neuronal ceroid lipofuscinosis (NCL) is a relatively common group of inherited neurodegenerative disorders characterised by the accumulation of autofluorescent lipopigments (ceroid) similar to lipofuscin. Because of this property, studies have concentrated on fatty acid metabolism and lipid peroxidation. METHODS: In the present study, the fatty acid composition of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and the molecular species compositions of diacylglycerophosphocholine (diacyl GPC), diacylglycerophosphoethanolamine (diacyl GPE) and alkenylacyl GPE (plasmalogens) were investigated in cultured skin fibroblasts from three patients with a confirmed diagnosis of the late infantile form of the disease (LINCL, CLN2) and three healthy age-matched controls. RESULTS: Relatively minor differences in the fatty acid compositions of PC and PE were observed between patients and controls. However, dimethyl acetals of plasmalogens were found to be 40% higher in the patients compared to in the controls. Control and LINCL fibroblasts displayed only slight differences in the molecular compositions of diacyl GPE and diacyl GPC. In contrast, compared with normal cells, LINCL fibroblasts had higher levels of alkenylacyl GPE species containing both 18 : 1 and polyunsaturated fatty acids, but lower levels of species with 16 : 0 or 18 : 0 in the sn-1 position. CONCLUSION: The molecular composition of PC and PE subclasses in skin fibroblasts of healthy subjects and patients suffering from LINCL is here described for the first time. While few differences are noticeable in the fatty acid composition of PC and PE and the molecular species distribution of diacylGPC and diacylGPE, the alkenylacyl GPE (or ethanolamine plasmalogens) were found to differ significantly between patients and healthy controls.
Subject(s)
Neuronal Ceroid-Lipofuscinoses/metabolism , Phospholipids/chemistry , Phospholipids/metabolism , Cells, Cultured , Child , Child, Preschool , Fatty Acids/analysis , Fibroblasts , Humans , Matched-Pair Analysis , Phosphatidylcholines/chemistry , Phosphatidylethanolamines/chemistry , Plasmalogens/chemistry , Tripeptidyl-Peptidase 1ABSTRACT
Phospholipid (PL) compositions and fatty acid (FA) patterns of PL were determined in the erythrocytes and blood thrombocytes of a seabird, the king penguin, living in the subantarctic area and feeding on prey rich in n-3 polyunsaturated FA. Results were compared between birds in three different physiological states (breeding and molting adults, chicks) to those reported for other birds. In erythrocytes, the ratios of cholesterol to PL and of sphingomyelin to phosphatidylcholine (PC) were lower than in other birds. The PL distribution was similar to those previously reported in the hen and pigeon. In contrast to other birds, cardiolipin levels were unexpectedly high (4%). Very long chain n-3 FA were abundant (13-27%) in phosphatidylethanolamine (PE), phosphatidylserine and PC, probably in relation to the natural diet of these birds. Among n-3 FA, 22:6n-3 was the most abundant in all PL (2-20%), whereas the highest levels of arachidonic acid were observed in PE (14%). In thrombocytes, the PL distribution and FA composition of the main PL (PC, PE) differed from those of erythrocytes, and in particular, levels of n-3 FA (9-12%) were 1.5-2 times lower. The highest levels of arachidonic acid were found in phosphatidylinositol (24%). The lipid profile of penguin erythrocytes could contribute to the efficiency of blood circulation and oxygen delivery in microvascular beds, thus favoring diving capacity of these animals. Our observations do not support the hypothesis of a common origin of avian thrombocytes and erythrocytes.
Subject(s)
Birds/blood , Blood Platelets/chemistry , Erythrocytes/chemistry , Lipids/blood , Animals , Antarctic Regions , Arachidonic Acid/blood , Cholesterol/blood , Dietary Fats, Unsaturated/administration & dosage , Fatty Acids/blood , Fatty Acids, Omega-3/administration & dosage , Female , Male , Phosphatidylcholines/blood , Phosphatidylethanolamines/blood , Phosphatidylserines/blood , Phospholipids/blood , Sphingomyelins/bloodABSTRACT
Erythrocyte and blood platelet phospholipid compositions were studied in three elephant seals and two fur seals, two species of marine mammals living in the Subantarctic region feeding on preys rich in (n-3) polyunsaturated fatty acids. Results were compared with those reported for related species and humans. In erythrocytes, the phospholipid (PL) and cholesterol (CHOL) contents were lower in pinnipeds than in humans. Phosphatidylcholine (PC) levels were higher in elephant seals than in fur seals, with a reverse trend for phosphatidylethanolamine (PE) and phosphatidylserine (PS). Both species had lower SM/PC ratios and PE plasmalogen concentrations than human. Erythrocytes were richer in (n-3) fatty acids (FA) in pinnipeds than in humans. In platelets, the PL content was lower and the CHOL content higher in elephant seals than in humans or in other phocid seal species studied to date. The SM/PC ratio was much higher than in other seal species or in man. In both species, the proportion of PE plasmalogens was higher in platelets than in erythrocytes. PL were more saturated in elephant seals than in fur seals. These results suggest that the erythrocytes and platelets of wild marine mammals may prove useful models to study the influence of dietary lipids on the structure and hemostatic function of these cells.
Subject(s)
Blood Platelets/chemistry , Erythrocytes/chemistry , Fur Seals/blood , Phospholipids/blood , Animals , Cholesterol/blood , Fatty Acids/blood , Humans , Phosphatidylcholines/blood , Phosphatidylethanolamines/blood , Phosphatidylserines/bloodSubject(s)
Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Hypercholesterolemia/drug therapy , Kidney Transplantation/physiology , Lipids/blood , Postoperative Complications , Pyridines/therapeutic use , Apolipoproteins/blood , Cholesterol/blood , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cyclosporine/therapeutic use , Drug Therapy, Combination , Female , Humans , Immunosuppressive Agents/therapeutic use , Male , Middle Aged , Triglycerides/bloodABSTRACT
The lipid content of cultured cells can be experimentally modified by supplementing the culture medium with specific lipids or by the use of phospholipases. In the case of the insulin receptor, these methods have contributed to a better understanding of lipid disorder-related diseases. Previously, our laboratory demonstrated that experimental modification of the cellular lipid composition of an insulin-sensitive rat hepatoma cell line (ZHC) resulted in an alteration in insulin receptor binding and biological action (Bruneau et al., Biochim. Biophys. Acta 928 (1987) 287-296/297-304). In this paper, we have examined the effects of lipid modification in another hepatoma cell line, HepG2. Exogenous linoleic acid (LA, n-6), eicosapentaenoic acid (EPA, n-3) or hemisuccinate of cholesterol (CHS) was added to HepG2 cells, to create a cellular model in which membrane composition was modified. In this model, we have shown that: (1) lipids were incorporated in treated HepG2 cells, but redistributed differently when compared to treated ZHC cells; (2) that insulin signaling events, such as insulin receptor autophosphorylation and the phosphorylation of the major insulin receptor substrate (IRS-1) were altered in response to the addition of membrane lipids or cholesterol derived components; and (3) different lipids affected insulin receptor signaling differently. We have also shown that the loss of insulin receptor autophosphorylation in CHS-treated cells can be correlated with a decreased sensitivity to insulin. Overall, the results suggest that the lipid environment of the insulin receptor may play an important role in insulin signal transduction.
Subject(s)
Lipids/pharmacology , Receptor, Insulin/metabolism , Animals , Cell Membrane/drug effects , Cell Membrane/metabolism , Cholesterol/analysis , Cholesterol Esters/pharmacology , Eicosapentaenoic Acid/pharmacology , Fatty Acids/analysis , Linoleic Acid/pharmacology , Lipids/isolation & purification , Membrane Fluidity/drug effects , Rats , Signal Transduction , Triglycerides/analysis , Tumor Cells, CulturedABSTRACT
The antithrombotic effects of dietary lipids were investigated in rat models of arterial and venous thrombosis. In the arterial model, thrombus formation was evaluated by determination of the occlusion time and the deposition of 111In-labeled platelets and 125I-labeled fibrinogen in a collagen-coated glass capillary inserted into an arterio-arterial shunt. Venous thrombosis was evaluated by measurement of the thrombus weight after administration of thromboplastin as a source of tissue factor and establishment of stasis in the vena cava. Diets were supplemented with saturated (SAT group) or (n-3) fatty acids, the latter being added either as MaxEPA oil (MaxEPA group), or as docosahexaenoic (DHA group) or eicosapentaenoic (EPA group) ethyl ester. Only the MaxEPA group displayed a prolonged occlusion time as compared with all other groups. Platelet accumulation, similar in the MaxEPA, EPA and DHA groups (13.3, 16.7 and 17.7 x 10(6) platelets/shunt, respectively), was significantly higher in the SAT group (25.3 x 10(6) platelets/shunt), while accumulation of fibrinogen-fibrin was similar whatever the group. There was a trend towards a lower venous thrombus weight in MaxEPA fed rats relative to those fed other diets. Our data indicate that the MaxEPA diet had antithrombotic effects in arterial and to a lesser extent venous thrombosis models, best attributed to its multiple targeting of platelets and coagulation.
Subject(s)
Fatty Acids, Omega-3/therapeutic use , Fibrinolytic Agents/therapeutic use , Thrombosis/drug therapy , Venous Thrombosis/drug therapy , Animals , Disease Models, Animal , Docosahexaenoic Acids/therapeutic use , Eicosapentaenoic Acid/therapeutic use , Fibrinogen/metabolism , Male , Platelet Count , Rats , Rats, WistarABSTRACT
The effects of dietary lipids on haemostasis were investigated in rats fed high fat diets enriched in saturated fatty acids (SAT), oleic acid (OLEIC), MaxEPA oil (MaxEPA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) and results were compared to those for rats fed standard chow (ST). Coagulant activities of factor IIc and factor VII-Xc were reduced by about 70% in the MaxEPA group and 50% in the EPA and DHA groups relative to the OLEIC, SAT and ST groups. Liver vitamin K levels were five times lower in the experimental groups than in the ST group, which would indicate an effect of high fat diets on vitamin K metabolism. However, only (n-3) fatty acids prolonged the prothrombin time. These components could act at the post-translational modification level of vitamin K-dependent plasma clotting factors. The changes in haemostatic factors found in the MaxEPA group were counteracted by vitamin K supplementation.
Subject(s)
Blood Coagulation Factors/metabolism , Blood Coagulation/drug effects , Dietary Fats, Unsaturated/pharmacology , Dietary Fats/pharmacology , Fatty Acids, Omega-3/pharmacology , Vitamin K/pharmacology , Animals , Docosahexaenoic Acids/pharmacology , Drug Combinations , Eicosapentaenoic Acid/pharmacology , Factor VII/metabolism , Lipids/blood , Liver/metabolism , Male , Oleic Acid/pharmacology , Prothrombin/metabolism , Rats , Rats, Wistar , Vitamin K/metabolismABSTRACT
A sensitive procedure is described for the simultaneous determination of vitamin E and coenzyme Q homologues and alpha-tocopherol oxidation products using two-isocratic step high pressure liquid chromatography (HPLC) and electrochemical detection in the oxidative mode. Zinc-catalyzed reduction in a post-column reactor allows the detection of alpha-tocopherolquinone, epoxy-tocopherolquinone, and ubiquinones. This technique was used to quantify lipophilic antioxidants in the liver tissue of rats treated or not with alpha-tocopherolquinone and in a plant oil. Alpha-tocopherolquinone and its epoxide derivatives, formed from alpha-tocopherol during iron-catalyzed phospholipid peroxidation, were also determined in a liposome suspension. The high selectivity and sensitivity of the coulometric detection system enabled use of low oxidation potentials giving little baseline noise, while a fast isolation procedure and quantitative recoveries of all oxidized and reduced forms made it possible to measure a high ubiquinol/ubiquinone ratio in liver tissue. Administration of alpha-tocopherolquinone to rats did not alter the antioxidant status of the liver, despite strong accumulation of both this quinone and its reduced form, alpha-tocopherolhydroquinone. These results indicate the presence of an efficient reductase and suggest that it could contribute to the protection of cellular membranes from oxidative stress.
Subject(s)
Antioxidants/analysis , Chromatography, High Pressure Liquid/methods , Liver/chemistry , Ubiquinone/analysis , Vitamin E/analysis , Vitamin E/chemistry , Animals , Chromatography, High Pressure Liquid/standards , Drug Stability , Iron/chemistry , Lipid Peroxidation , Liposomes/chemistry , Liver/drug effects , Male , Plant Oils , Rats , Rats, Wistar , Reproducibility of Results , Ubiquinone/analogs & derivatives , Vitamin E/analogs & derivatives , Vitamin E/pharmacology , Zinc/chemistryABSTRACT
Chromatin phospholipidic fraction, as previously demonstrated, shows the same localization as RNA inside the nuclei. DNase and RNase treatment of nuclei removed almost totally the DNA, 63% of RNA and caused a 50% loss of phospholipids. The aim of the present investigation is to study the fraction of RNase undigested nuclear RNA and its relationship with the phospholipids still present in the nuclei. Isolated hepatocyte nuclei were treated with Triton X-100 and digested with RNase and DNase. The undigested nuclear material contained proteins (98%) and a small amount of RNA (1.7%), DNA (0.4%) and phospholipids (0.18%). The analysis of phospholipids showed the presence of two components only, namely phosphatidylcholine and sphingomyelin. In the same complex, the activity of sphingomyelin synthase, phosphatidylcholine-dependent phospholipase C and neutral sphingomyelinase has been detected. Treatment of isolated RNA with neutral sphingomyelinase modified the RNA in RNase sensitive RNA, thus suggesting that the SM may represent a bridge between two RNA strands possibly regulating transcription.
Subject(s)
Cell Nucleus/metabolism , RNA/metabolism , Ribonucleases/metabolism , Sphingomyelins/metabolism , Animals , Cell Nucleus/enzymology , Cells, Cultured , Female , Liver/cytology , Liver/enzymology , Liver/metabolism , Male , Phosphatidylcholines/metabolism , Rats , Rats, Sprague-Dawley , Sphingomyelin Phosphodiesterase/metabolism , Type C Phospholipases/metabolismABSTRACT
Human platelet CD38 is a multifunctional ectoenzyme catalysing the synthesis and hydrolysis of cADP-ribose (cADPR), a recently identified calcium-mobilizing agent that acts independently of D-myo-inositol 1,4,5-trisphosphate and is known to be expressed by human platelets. The present work shows that ADP-ribosyl cyclase activity is exclusively a membrane activity, of which the major part is located in plasma membranes and a small part in internal membranes. In broken cells, cyclase activity was insensitive to the presence of calcium and was not modulated by agonists such as thrombin or ADP, whereas in intact cells thrombin increased cADPR formation by 30%, an effect due to fusion of granules with the plasma membrane. In order to assess the role of cADPR as a calcium-mobilizing agent, vesicles were prepared from internal membranes and loaded with 45CaCl2. These vesicles were efficiently discharged by IP3 in a dose-dependent manner, but were not responsive to cADPR or ryanodine in the presence or absence of calmodulin. Thus cADPR is unlikely to play a role in intracellular calcium release in human blood platelets.
Subject(s)
Adenosine Diphosphate Ribose/blood , Antigens, CD , Blood Platelets/metabolism , Calcium/blood , ADP-ribosyl Cyclase , ADP-ribosyl Cyclase 1 , Adenosine Diphosphate/pharmacology , Antigens, Differentiation/blood , Blood Platelets/drug effects , Calcimycin/pharmacology , Calcium Chloride/pharmacology , Cell Membrane/enzymology , Flow Cytometry , Humans , Hydrolysis , Inositol 1,4,5-Trisphosphate/pharmacology , Magnesium Chloride/pharmacology , Membrane Glycoproteins , NAD+ Nucleosidase/blood , Ryanodine/pharmacology , Second Messenger Systems , Thrombin/pharmacologyABSTRACT
The influence of dietary (n-3) compared with (n-6) polyunsatured fatty acids (PUFA) on the lipid composition and metabolism of adipocytes was evaluated in rats over a period of 1 week. Isocaloric diets comprised 16.3 g/100 g protein, 53.8 g/100 g carbohydrate and 21.4 g/100 g lipids, the latter containing either (n-3) PUFA (32.4 mol/100 mol) or (n-6) PUFA (37.8 mol/100 mol) but having identical contents of saturated, monounsaturated and total unsaturated fatty acids and identical polyunsaturated to saturated fatty acid ratios and double bond indexes. Despite comparable food intake, significantly smaller body weight increments and adipocyte size were observed in rats of the (n-3) diet group after feeding for 1 wk. Rats fed the (n-3) diet also had significantly lower concentrations of serum triglycerides, cholesterol and insulin compared with those fed the (n-6) diet, although levels of serum glucose and free fatty acids did not differ in the two dietary groups. In the (n-6) diet group, the (n-6) and (n-3) PUFA contents of plasma triglycerides, free fatty acids and phospholipids were 30-60% higher and 60-80% lower, respectively, than in the (n-3) diet group, whereas adipocyte plasma membrane phospholipids showed a significantly higher unsaturated to saturated fatty acid ratio and greater fluidity. Glycerol release in response to noradrenaline was significantly higher in the adipocytes of rats fed the (n-3) diet, whereas the antilipolytic effect of insulin generally did not differ in the two groups. Finally, insulin stimulated the transport of glucose and its incorporation into fatty acids to a lesser extent in adipocytes of (n-3) diet fed rats compared with (n-6) diet fed rats. This reduction in the metabolic effects of insulin in rats fed a (n-3) diet for 1 wk could be related to smaller numbers and a lower binding capacity of the insulin receptors on adipocytes and/or to a lesser degree of phosphorylation of the 95 kDa beta subunit of the receptor. In conclusion, dietary intake for 1 wk of (n-3) rather than (n-6) PUFA is sufficient to induce significant differences in the lipid composition and metabolic responses to insulin of rat adipocytes.
Subject(s)
Adipocytes/drug effects , Adipocytes/metabolism , Fatty Acids, Omega-3/pharmacology , Fatty Acids, Unsaturated/pharmacology , Fatty Acids/metabolism , Insulin/pharmacology , Animals , Cell Membrane/metabolism , Fatty Acids, Omega-6 , Insulin/metabolism , Male , Phosphorylation , Rats , Rats, Wistar , Time Factors , Tyrosine/metabolismABSTRACT
The aim of the present study was to determine whether clopidogrel, one of the most potent antiplatelet compounds in vivo, could alter the lipid composition of plasma, liver tissue or platelet membranes in the rat. Animals treated (10 mg/kg per day for 7 days) with clopidogrel and its inactive analogue (R form, SR 25989) were compared with control animals. Neither compound altered plasma concentrations of triglycerides or free and esterified cholesterol, and no changes were observed in liver lipids. Clopidogrel treatment significantly lowered platelet cholesterol content and cholesterol to phospholipid ratio, while SR 25989 had comparatively smaller effects. Concerning platelet phospholipids, clopidogrel treatment reduced phosphatidylcholine(PC) but increased sphingomyelin (SP) content, whereas SR 25989 lowered PC and phosphatidylserine (PS) but raised phosphatidylethanolamine (PE) content. A significant increase in the arachidonic acid content of PE was observed only in the SR 25989 group. Clopidogrel and SR 25989 both induced an increase in the unsaturation level of platelet PC, accompanied by a decrease in the level of unsaturation in platelet SP, while a similar decrease was observed for phosphatidylinositol only in the clopidogrel group. These changes in platelet membrane composition in the clopidogrel group are probably unrelated to the antiaggregating properties of the drug, but could influence other platelet functions under long-term treatment.
ABSTRACT
OBJECTIVE: To examine the possible involvement of an increase in diet-induced thermogenesis from brown adipose tissue (BAT) in the n-3 polyunsaturated fatty acids (n-3 PUFA) induced limitation of the development of white fat pads during high-fat feeding. DESIGN: Rats fed for four weeks on a low-fat/high-carbohydrate diet (C group) or high-fat diet without n-3 PUFA (REF group), with eicosapentaenoic acid (EPA group), with docosahexaenoic acid (DHA group) or with a mixture of these two fatty acids (MIX group). MEASUREMENTS: Epididymal and retroperitoneal fat pad mass, BAT composition, Guanosine 5'-diphosphate (GDP) binding and uncoupling protein (UCP) content were measured in the five groups of rats. RESULTS: The masses of retroperitoneal and epididymal white fat pads were lower in the groups fed n-3 PUFA than in the C and REF groups. The total BAT GDP binding was 1.6 times higher in the MIX and EPA groups than in the REF group. The BAT from the EPA group presented an enrichment in mitochondria compared to the C and REF groups whereas the BAT from the DHA and REF groups presented a hyperplasia and an increase in thermogenic activity of the mitochondria compared to the C group. The higher thermogenic activity of BAT was observed in the MIX group and is due to hyperplasia and to an increase in thermogenic activity of mitochondria. CONCLUSIONS: n-3 PUFA induce a marked stimulation of BAT thermogenic activity without changes in the UCP content compared to a high-fat diet without n-3 PUFA. The mixture of EPA and DHA has the more pronounced effect while EPA and DHA seem to act in synergy on BAT thermogenesis via different mechanisms.
Subject(s)
Adipose Tissue, Brown/metabolism , Animal Nutritional Physiological Phenomena , Dietary Fats/administration & dosage , Fatty Acids, Omega-3/administration & dosage , Hot Temperature , Adipose Tissue/metabolism , Adipose Tissue, Brown/enzymology , Animals , Electron Transport Complex IV/metabolism , Energy Intake , Lipid Metabolism , Male , Proteins/metabolism , Rats , Rats, WistarABSTRACT
A reliable procedure is described for the joint analysis of vitamin E (tocopherols), cholesterol and phospholipids in the same minute sample of human platelets and on human cultured endothelial cells. The whole procedure is based on the extraction of total lipids, thin-layer chromatography of all compounds of interest and microcolumn purification of tocopherols and cholesterol. The combined use of butyl hydroxytoluene and ascorbic acid in the purification steps allowed a complete recovery of the tocopherols analyzed, as well as of cholesterol by high-performance liquid chromatography. The detection of these lipids was performed with fluorometric, spectrophotometric and evaporative light-scattering detectors whose respective sensitivities were compared. The fatty acid composition of phospholipid classes from the same sample, separated on the same silica gel plate, was determined by gas-liquid chromatography. The whole procedure is rapid since it requires about 4 h to analyse tocopherols and cholesterol and to prepare methylated fatty acids, 28 samples being easily completed within one working day. The evaluation of the whole membrane antioxidant status requires as little as one 25 cm2 confluent culture flask (about 0.75 x 10(6) cells) for endothelial cells or two ml of blood (3 x 10(8) platelets).
Subject(s)
Blood Platelets/chemistry , Cholesterol/analysis , Endothelium, Vascular/chemistry , Fatty Acids/analysis , Phospholipids/chemistry , Vitamin E/analysis , Cells, Cultured , Cholesterol/blood , Chromatography, Gas , Chromatography, High Pressure Liquid/methods , Chromatography, Thin Layer/methods , Endothelium, Vascular/cytology , Fatty Acids/blood , Fatty Acids/classification , Humans , Reproducibility of Results , Sensitivity and Specificity , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Vitamin E/bloodABSTRACT
The human P2Y1 purinoceptor has been expressed in Jurkat cells and the effects of HPLC purified nucleotides on calcium movements were measured. The most potent agonist was 2-methylthio-ADP followed by ADP. ATP, Sp-ATPalphaS and beta,gamma-methylene-ATP were competitive antagonists. Suramin and PPADS inhibited the effects of ADP. This pharmacological profile is the same as that of the so-called P2T purinoceptor responsible for platelet aggregation, which has not yet been identified. Using PCR we found the P2Y1 receptor to be present in blood platelets and megakaryoblastic cell lines. These data suggest that the P2Y1 receptor may be the elusive P2T receptor.
Subject(s)
Adenosine Triphosphate/pharmacology , Blood Platelets/metabolism , Megakaryocytes/metabolism , Membrane Proteins , Receptors, Purinergic P2/biosynthesis , Receptors, Purinergic P2/metabolism , Adenosine Diphosphate/isolation & purification , Adenosine Diphosphate/metabolism , Adenosine Diphosphate/pharmacology , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/isolation & purification , Adenosine Triphosphate/metabolism , Binding, Competitive , Blood Platelets/drug effects , Calcium/metabolism , Chromatography, High Pressure Liquid , Dose-Response Relationship, Drug , Humans , Jurkat Cells/drug effects , Jurkat Cells/metabolism , Megakaryocytes/drug effects , Polymerase Chain Reaction , Pyridoxal Phosphate/analogs & derivatives , Pyridoxal Phosphate/pharmacology , Receptors, Purinergic P2/drug effects , Receptors, Purinergic P2Y1 , Receptors, Purinergic P2Y12 , Suramin/pharmacologyABSTRACT
[125I]TID [3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine] is a commercially available, hydrophobic, photoactivatable, gamma-emitting reagent mostly used to label protein hydrophobic domains. It has also been used to radiolabel the phospholipids of lung surfactant (Gilliard et al., Anal. Biochem. 193, 310-315, 1991). Since a nonspecific, highly sensitive, lipid-labeling probe would be a very useful tool to investigate lipid-protein interactions in biological membranes, we characterized further the [125I]TID-labeling products of lipids from cultured Chinese hamster ovary cells (IR-CHO). After labeling of whole cells, TLC analysis followed by autoradiography enabled detection of sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, cardiolipin, diglycerides, cholesterol and its esters, and triglycerides. Analysis of the radioactivity associated with the saponification products of different lipids showed that [125I]TID was mostly (80%) extracted with the fatty acid moiety of the lipids whereas 20% remained associated with the hydrosoluble moiety. Similar radioactivity profiles were observed after labeling of whole cells or extracted and liposome-reconstituted lipids; the [125I]TID probe was able to diffuse in all intracellular organelles. Labeling was not equivalent between the different lipid classes, and it appeared that the amount of associated radioactivity correlated well with the degree of lipid unsaturation. This was confirmed by studying [125I]TID incorporation in phosphatidylcholines of different chain length and unsaturation. Taken together, our data demonstrate that [125I]TID can be used as a radiolabel for lipids in cultured cells. It is rapidly incorporated in the hydrophobic part of membranes, diffuses into all cellular compartments, and labels all lipid classes, including phospholipids, cholesterol, and glycerides, with a sensitivity in the nanomolar range.