Oral supplementation with Scabiosa artropurperea L. aqueous extract improves the in vivo sexual behaviour, sperm quality and androgen level in rams.

The effects of an aqueous extract of Scabiosa atropurpurea L. (AES) on the reproduction potential of Queue Fine de l'Ouest rams were evaluated over 9 weeks. Eighteen mature (4-6 years old) rams (52.8 ± 2.6 kg) were divided into three groups. The control (C) group was fed oat hay ad libitum with 700 g of concentrate and the other two groups were fed the same diet supplemented with AES at 1 and 2 mg/kg body weight (AES1 and AES2, respectively). Ram sperm was collected with an artificial vagina (2 × 2 days/week) to evaluate sperm production and quality, antioxidant activity, the adenosine triphosphate (ATP) and calcium concentrations. Sexual behaviour and plasma testosterone concentrations were also investigated. The administration of AES improved sexual behaviour (the duration of contact and the number of lateral approaches). The addition of AES also improved individual spermatozoa motility (C: 71.7% ± 6.3%; AES1: 78.3% ± 4.9%; AES2: 83.8% ± 4.4%), the sperm concentration (C: 5.6 ± 0.36; AES1: 6.4 ± 0.81; AES2: 6.7 ± 0.52 × 109 spermatozoa/mL), the ATP ratio (C: 1 ± 0.08; AES1: 2.1 ± 0.08; AES2: 3.3 ± 0.08) and the calcium concentration (C: 5.6 ± 0.24; AES1: 7.7 ± 0.21; AES2: 8.1 ± 0.24 mmol/L). AES treatment decreased the percentage of abnormal sperm (C: 18.5% ± 1.2%; AES1: 16.2% ± 1.1%; AES2: 14.8% ± 0.94%) and DNA damage (C: 62%; AES1: 27%; AES2: 33%) and was associated with elevated seminal fluid antioxidant activity (C: 22 ± 0.27; AES1: 27.1 ± 1.08 and AES2: 27.5 ± 0.36 mmol Trolox equivalents/L) and plasma testosterone (C: 8.3 ± 0.7; AES1: 11.7 ± 0.4; AES2: 15 ± 0.7 ng/L). In conclusion, our study suggests that S. atropurpurea may be potentially useful to enhance libido and sperm production and quality in ram.

Analysis of Management Practices and Breeders' Perceptions of Climate Change's Impact to Enhance the Resilience of Sheep Production Systems: A Case Study in the Tunisian Semi-Arid Zone.

Global climate change inflicts unambiguous risks on agricultural systems and food security. Small ruminants are known for their ability to adapt to changing environmental conditions. This paper aims to characterize sheep production systems in a vulnerable agricultural zone and the breeders' perceptions of climate change to apprehend challenges that they are confronting, and formulate resilience actions. The data analysis is based on 94 semi-structured surveys of sheep farmers carried out in the Tunisian semi-arid region. The PCA analysis results revealed three main sheep production systems. The agro-pastoral rain-fed system (AGPRF) is dominant (55%), with large farms and common pastures integrating cereals and fodder. The agro-pastoral irrigated system (AGPI: 20%) is characterized by small-area and forage irrigation (1.8 ha) and a smaller number of ewes but a greater use of animal feed supplementation. The agro-sylvo-pastoral system (AGSP: 25%) is a system where grazing is based on common lands and using tree sub-products, while the agricultural area is exclusively used to cultivate cereal crops. Sheep breeders' climate perceptions are summarized as unpredictable climate events, a decrease in precipitation, and an increase in temperature. Resilience actions principally consist of reducing flocks' numbers, using alternative local feed, fodder, and water resources, and building more shelters and planting more trees in the grazing areas. Nevertheless, cost-effectiveness should be considered in such vulnerable zones to insure the sheep production systems' sustainability.

Impact of Body Reserves Dynamic on Productivity and Reproductive Performance in Fat-Tail and Thin-Tail Sheep Breeds over Contrasting Production Cycles.

Under climate change, animal breeding faces severe nutritional challenges, exhibiting their resilience and efficiency to survive and produce. The animals' robustness can be evaluated through the use and reconstitution of body reserves (BR). This study investigated the dynamics of ewes' BR, measured through body weight (BW) and the body condition score (BCS), and their effect on production performances of 480 ewes belonging to fat-tailed Barbarine (BB) and thin-tailed Queue Fine de l'Ouest (QFO) ewes over two consecutive years. The BW and BCS were recorded across physiological stages. Reproductive parameters and lambs' growth were calculated. For both years and breeds, the decrease in the BCS between mating and lambing showed BR mobilization to balance nutritional restrictions, which continues until suckling; after weaning, a BR accretion was noted. The lambs' birth weight was similar regardless of the dams' BCS at lambing, though it was lower in the second year (3.5 vs. 4 kg). Lambs' growth parameters depended on ewes' BCS at lambing and weaning. Fertility rates changed with BCS changes and the higher values (80%) were recorded for ewes with BCSs between 2 and 2.5. However, prolificacy was similar for both breeds and years (p > 0.05). In conclusion, ewes have developed adaptative capacities through BR mobilization to cope with environmental challenges and successfully rear their offspring. To maintain a high offspring growth rate, it is recommended to plan correct flock nutrition during suckling.

Comparative Study of Carcass Characteristics and Meat Quality of Local Mediterranean Donkey Breeds.

This study aimed to evaluate carcass and meat quality traits in Masri (n = 14) and North African (n = 14) male donkeys, raised in a semi-intensive breeding system, grazing on mountainous areas, with supplementation of 1 kg of barley per day per animal, slaughtered at 5 (±0.48) years old. Compared to Masri, the North African population exhibited higher (p < 0.05) final body weights (181.7 ± 2.1 and 212.5 ± 7.6 kg) and cold carcass weights (101.7 ± 1.3 and 116.2 ± 4.5 kg), but lower (p < 0.05) cold dressing percentages (56.0 ± 0.4 and 54.6 ± 0.4%). Meat quality analyses showed higher (p < 0.05) cooking loss values in Masri meat (43.9 ± 0.8 vs. 39.9 ± 1.2%). Among the meat color parameters, the hue value was higher (p < 0.05) in North African samples (0.42 ± 0.01 vs. 0.39 ± 0.1). Meat from North African donkeys had higher (p < 0.05) dry matter, fat, and protein contents. Meat amino acid analysis revealed abundant levels of lysine, leucine, and methionine, in both populations. Donkey meat from both populations presented a high polyunsaturated fatty acids content, resulting in polyunsaturated fatty acids/saturated fatty acids and omega 6/omega 3 ratios for all breeds close to the recommended values for human health. Atherogenic and thrombogenic indices were also suggested to have positive effects on consumers' artery health. The characteristics of donkey meat present intriguing nutritional aspects compared to ruminant meat, and its production should be encouraged in the rural development of inner-Mediterranean areas.

Evaluation of acute toxicity of Scabiosa artropurperea var.maritima aqueous extracts in Swiss mice.

Scabiosa artropurperea var.maritima is a plant widely distributed in the Mediterranean region and used as a traditional medicine. The present study evaluated the biochemical composition and the potential toxicity of aqueous extract of whole Scabiosa artropurperea var.maritima through acute toxicity oral administration in male mice. Phytochemical analysis of the Scabiosa artropurperea var.maritima revealed high levels of reductor sugars and significant flavonoid and total phenol content. The aqueous extract of Scabiosa artropurperea var.maritima was daily oral administered to mice at doses of 300 (group 1), 2000 (group 2) and 4000 (group 3) mg/kg body weight per day for 14 days. We observed no significant difference in the consumption of food, body weight and relative organ weights except for an increase in the seminal vesicles weight in group 3. Hematological parameters revealed the non-adverse effects of prolonged oral consumption of Scabiosa artropurperea var.maritima except for a slight increase but significant of percentage of hematocrit in group 1 and 3 and a decrease in percentage of granulocytes in group 2. The histopathologic examination did not show any differences in vital organs. We also observed non-adverse effects on the reproductive parameters including testosterone concentration, spermatozoa motility and morphologies. Based on our findings, the aqueous extract of Scabiosa artropurperea var.maritima could be considered safe for oral medication in animals.

Substitution of soyabean meal by faba bean improves body weight, sperm production and testosterone concentration in mature Queue Fine de l'Ouest rams.

The current study aimed at investigating the effects of partial or total substitution of soybean meal with faba bean on reproductive parameters of Queue Fine de l'Ouest rams. Eighteen adult rams (49.8 ± 3.7 kg and 2.4 ± 1.5 years old) were divided into three homogenous groups. Rams received oat hay ad libitum and 3 types of concentrate (33 g/BW0.75) where soybean meal was the main protein source (SBM diet, n = 6), partially (50%, SBMFB diet, n = 6) or totally (100% FB diet, n = 6) substituted in nitrogen basis by local faba bean. The volume of ejaculate, sperm concentration and sperm mortality rate were measured weekly by collecting semen using an artificial vagina. Serial blood samples were collected 30 days and 120 days after the beginning of the experiment in order to assess plasma testosterone concentrations. The results showed that hay intake was affected (P < 0.05) by the nature of the nitrogen source incorporated (1032.3 ± 12.2; 1026.8 ± 56.6 and 972.8 ± 39.05 g DM/d for SBM, FB and SBMFB respectively. The average live weight of the rams increased from 49.8 ± 0.4 (week 1) to 57.3 ± 0.9 kg (week 17) without effect of the diet. A positive effect of faba bean incorporation in the concentrate was observed on ejaculate volume, concentration and spermatozoa production. All these parameters were significantly higher in SBMFB and FB groups than in SBM group (P < 0.05). The protein source did not affect the percentage of dead spermatozoa and the total abnormalities which were similar for the three diets (38.7; 35.8 and 38.1% for SBM; SBMFB and FB, respectively). The mean testosterone concentration was higher (P < 0.05) in rams fed with faba bean (1.7 ± 0.7 to 1.9 ± 0.7 ng/ml for the SBMFB and the FB groups respectively) than in rams fed SBM diet (1.06 ± 0.5 ng/ml). It was concluded that the substitution of soybean meal by faba bean improved the reproductive performance and did not affect sperm quality in Queue Fine de l'Ouest rams.

Vulnerability assessments in dairy cattle farms based on individual sensitivity to heat stress.

Climate change (CC) is expected to increase temperatures and the frequency of extreme weather events, which renewed interest in heat stress (HS) effects on dairy cattle farms. Resilience is a key concept that should be considered to better understand the dairy farms exposure to HS and to combat CC-related risks. Thus, this study aimed to investigate the aspects of HS vulnerability for Tunisian dairy cattle farming systems. Historical milk test-day records from official milk recording were merged with temperature and humidity data provided by public weather stations. Firstly, different models relying in two heat load indices were applied for HS exposure assessment. Secondly, broken line models were used to estimate HS thresholds, milk losses, and rates of decline of milk production associated with temperature-humidity index (THI) across parities. Thirdly, individual cow responses to HS estimated using random regression model were considered as key measures of dairy farming system sensitivity assessment to HS. Dairy farms are annually exposed for 5 months to high THI values above 72 in Tunisia. The tipping points, at which milk yield started to decline over parities with 3-day average THI, ranged between 65 and 67. The largest milk decline per unit of THI above threshold values was 0.135 ± 0.01 kg for multiparous cows. The milk losses estimated due to HS in the Holstein breed during the summer period (June to August) ranged between 110 and 142 kg/cow in north and south, respectively. A high HS sensitivity was proved especially in dairy farms characterized by large herd size and high milk production level. Hence, providing knowledge of dairy farms vulnerability to HS may provide the basis for developing strategies to reduce HS effects and plan for CC adaptation.

Effects of green oak acorn (Quercus ilex) intake on nutrient digestibility, lamb growth, and carcass and non-carcass characteristics.

The green oak (Quercus ilex) plays an important role in forest ecology when oaks are the dominant species or are plentiful. The use of acorns as an alternative to barley for livestock feeding can be beneficial for breeders. The aim of this study was the evaluation of the acorn intake by lambs in two stages, suckling and fattening, on growth, diet digestibility, carcass and non-carcass characteristics. For this, 32 lambs were used. During the suckling period, 16 lambs were reared on range pasture, supplied by barley (S-Ba), the other 16 on forest pasture and supplied by acorns (S-Ac). During the fattening period, lambs were assigned to concentrate based either on barley (F-Ba) or acorn (F-Ac) resulting in eight animals per suckling treatment per fattening treatment. The feed intake, diet digestibility and lamb growth were recorded. At 90 d of fattening, all animals were slaughtered and carcass traits studied. The main results show that the incorporation of acorn in concentrate was without effect on digestibility of organic matter, crud protein and neutral detergent fibre. The nitrogen balance was positive for animals fed barley concentrate or acorn one ( > 8  g d - 1 ). The lamb growth rates and slaughter body weight were not affected by acorn incorporation in both phases ( p > 0.05 ). Consequently, the carcass weights and carcass yields were similar. The F-Ac and S-Ac lambs had relatively heavier liver than F-Ba and S-Ba. The carcass composition in cutting pieces and that in tissues (muscle, fat and bone) was similar for all groups. These results suggest that acorns could replace partially conventional feedstuffs as concentrate without affecting animal performance and carcass quality.

Effects of Using Rosemary Residues as a Cereal Substitute in Concentrate on Vitamin E, Antioxidant Activity, Color, Lipid Oxidation, and Fatty Acid Profile of Barbarine Lamb Meat.

The shortage of some ingredients and, consequently, the continuous increase in the price of feed encourage the search for other alternatives to maintain animal production and enhance its products. In this line, the use of aromatic plant by-products in animal diet has been recently and widely considered, given their richness in bioactive compounds. Therefore, the aim of this study was to investigate the effects of the inclusion of rosemary residues (RR) and protein sources on lamb meat quality. The experiment was carried out on 24 male Barbarine lambs (3 months old) with an average body weight (BW) of 17.8 ± 2.6 kg, which were divided into three homogeneous groups according to BW. The diet comprised 600 g of oat hay and 600 g of concentrate. Three types of concentrate were evaluated: commercial concentrate as the control group (C); rosemary residues (RR) plus soybean meal as the RRS group, and RR plus faba bean as the RRF group. After an experimental period of 65 days, lambs were slaughtered. The inclusion of RR in both concentrates increased the α-tocopherol and total polyphenol content in meat and protected meat against discoloration (high red index and chroma after 9 days of storage) but did not affect meat lipid oxidation, which was similar for all groups. The FA profile was affected by the inclusion of RR, with no effect from the source of protein (faba bean or soybean). The inclusion of RR in the concentrate increased the C18:2 n-6, C18:3 n-3, C20:4 n-6, C20:5 n-3, and C22:5 n-3 content (p < 0.05). Consequently, the inclusion of RR also increased the total polyunsaturated FA (p < 0.05) and the ratio of polyunsaturated FA to saturated FA (p < 0.05). The results of this study demonstrate that concentrate based on RR could be useful for lamb meat production by improving the nutritional quality of meat, especially the fatty acid profile. In addition, soybean meal can be replaced by faba bean in lamb concentrate without affecting meat quality.

Carcass Traits and Meat Quality of Fat-Tailed Lambs Fed Rosemary Residues as a Part of Concentrate.

Facing climate change implications on feeds unavailability, unconventional resources are being considered with a growing interest such as aromatic plant distillation residues with a two-fold object, enhancing meat quality by increasing the antioxidant properties and reducing feed prices which are often imported though expensive. Hence, this study aims to assess the effects of rosemary distillation residues (RR) incorporation in concentrate associated to two nitrogen sources as a substitute for standard concentrate on lamb's growth, carcass traits and meat quality. For this, 24 Barbarine male lambs (3 months old, 17.83 ± 2.6 kg body weight) were divided into three groups. All lambs received individually 600 g of oat hay as roughage and 600 g of standard concentrate for control group, 600 g of concentrate based on RR and soybean meal for RRS group and 600 g of concentrate based on RR and faba bean for RRF group. After 65 days of experiment, all lambs were slaughtered. Phenolic and tocopherol intakes were significantly higher for both RR groups compared to control (p < 0.05). Growth, carcass weights, dressing percentages and non-carcass component weights were unaffected by the diet (p > 0.05). Moreover, regional and tissular compositions and meat physical properties were similar irrespective of the diet (p > 0.05). All color parameters were similar among groups (p > 0.05). However, meat produced by lambs receiving RR-based concentrate was richer on vitamin E and polyphenol contents than control lambs (p < 0.05). Rosemary by-products may substitute the standard concentrate resulting in similar lamb's growth and carcass traits, while improving meat quality by increasing vitamin E content, which could improve its antioxidant power.

Fatty acid profile of muscles and adipose tissues of fat-tail Barbarine lambs as affected by rosemary residue intake.

The rosemary distillation industry produces a considerable amount of rosemary distillation residues (RRs), which can be an alternative for feeding animals in harsh conditions and could enhance animal product quality. Given the meat quality is largely influenced by its fat content and fatty acid composition, the fatty acid (FA) profiles of longissimus thoracis et lumborum (LTL), semi-membranous (SM) muscles, and caudal (CFs) and omental fats (OFs) were determined using 21 Barbarine lambs fed with or without RRs. Diets contained 600 g of concentrate plus 600 g of forage. Forage represented oat hay, RR87 and RR60 pellets containing 87 % or 60 % of RR, respectively. At the end of the study, all lambs were slaughtered, and the fatty acid profile was studied. The inclusion of RR increased the polyunsaturated fatty acid (PUFA) contents and reduced saturated fatty acids (SFAs), and the thrombogenic and saturation indexes in all tissues. The SM muscle was the richest tissue in PUFAs, n -3 and n -6; however, both adipose tissues contained the highest proportions of SFAs. Especially the OF was the richest tissue in oleic acid and SFAs. Feeding RR to lambs enhanced meat quality.

Thermotolerance indicators related to production and physiological responses to heat stress of holstein cows.

Heat stress (HS) adversely influences dairy cattle welfare and productivity. This study aimed to investigate the effects of HS on production and physiological parameters of Holstein cows. Two experiments each lasted 6 weeks were conducted in four Tunisian farms, firstly during summer under HS (n = 80, THI = 77) and later during autumn under thermo-neutral (n = 80, THI = 54) conditions. Respiration rate (RR), skin temperature (ST), rectal temperature (RT) and milk yield were measured, and milk samples were collected on 2 days every week during each experimental period. Temperature and relative humidity were measured inside the barn to calculate the temperature-humidity index (THI). Mixed models were used to evaluate the effects of period and the relationships between THI and physiological and production traits. Reaction norm models were applied to quantify the individual responses of cows across the trajectory of THI during the HS period. A clustering methodology was developed to identify tolerant and sensitive cows to HS based on their slope for response of physiological and production traits during HS period. In summer, RR (61 breaths/min) and ST (37.7 °C) were 2.3- and 1.3-fold higher, whereas milk yield per milking was 24% lower compared with thermo-neutral conditions. Linear relationship between THI and RR, ST and RT was observed and showed increases by 2 breaths/min, 0.5 °C and 0.04 °C per increase in one THI unit, respectively. Inversely, milk, fat and protein yields showed a drop of 0.13 kg, 0.4 g and 0.3 g per milking per increase in one THI unit, respectively. Cows qualified to be heat tolerant by our work tended to have higher RR, ST, and RT and lower to almost no decay in milk yield compared to cows qualified to be heat sensitive. Specifically, RR could be used as a reliable indicator for thermotolerance. The results of this study deepen our understanding of different aspects of HS resilience.

Effects of Rosmarinus officinalis†L. essential oils supplementation on digestion, colostrum production of dairy ewes and lamb mortality and growth.

The aim of this study was to evaluate the effect of rosemary essential oils (REO) and the forage nature on ewes' performances, immune response and lambs' growth and mortality. Forty-eight dairy ewes (Sicilo-Sarde) were fed oat-hay or oat-silage supplemented with 400 g of concentrate during pregnancy and 600 g during postpartum. The experimental concentrate contained the same mixture as the control (barley, soybean meal and mineral vitamin supplement) more 0.6 g/kg of REO. Two groups were obtained with each forage (Hay groups: H-C and H-REO; Silage groups: S-C and S-REO). REO increased the dry matter (DM) intake, the nitrogen intake and retention being higher with the silage groups (P < 0.05). REO increased solid non-fat (P = 0.004) and fat contents of colostrum which was higher with hay (P = 0.002). REO decreased lamb mortality (P < 0.05) which averaged 21% for control groups and 6% for H-REO, while no mortality was recorded with S-REO. REO dietary supply improved forage intake and tended to ameliorate colostrum production; it could be a natural additive to improve ewes' performances.

Effects of Rosmarinus officinalis L. as essential oils or in form of leaves supplementation on goat's production and metabolic statute.

The effects of rosemary supply in form of essential oils (REO) or leaves (RL) on performances of goats were investigated. Thirty goats were allocated into three equal groups, which were fed oat-hay ad libitum and 400 g of concentrate during the two last weeks of pregnancy and 600 g during the first 8 weeks of lactation. Three-control diet (C) was a mixture of barley, soybean meal and mineral vitamin supplement. The experimental concentrates contained the same mixture of the control diet plus 0.6 g/kg of REO or its equivalent supply RL (60 g/kg). Rosemary supply did not affect dry matter (DM), organic matter (OM), crude protein (CP) and neutral detergent fiber (NDF) digestibility. While urinary nitrogen loss was higher for experimental groups than the C (P = 0.03). Daily milk production was significantly higher (P = 0.007) for rosemary groups (694 and 582 ml for RL and REO, respectively) than C group (442 ml). Rosemary decreased numerically (P > 0.05) the fat content (23, 25 and 26.5 g/l for REO, RL and C groups, respectively) but significantly increased the fat (P = 0.003) and protein content (P = 0.008). The growth rate of kids was significantly higher (P = 0.008) for RL (111 g) than that for REO and C (97 and 83 g, respectively). However, rosemary has not shown significant effect on the plasma metabolite concentrations. Given the facility to obtain the rosemary leaves, this form of rosemary use is recommended as natural alternative to improve the performances of goats.

The effects of diet, slaughter weight and docking on growth, carcass composition and meat quality of fat-tailed Barbarine lambs. A review.

This review summarises the main factors that influence meat production and quality in fat-tailed Barbarine (FTB) lambs. As a general feature, FTB lamb's growth is moderate, and the average daily gain ranges between 100 and 350 g. The carcass being relatively fatty, carcass fat content varies from 10% to 32%; white fat and rose meat are often dominant in these carcasses. The meat fatty acid profile of this fat-tailed breed is similar to that of thin-tailed ones, with a prevalence of palmitic, stearic and oleic acids. The order of dissected adipose tissues accumulation, estimated by allometry coefficients, is in agreement with observations in thin-tailed sheep. However, tail fat allometry coefficient is closer to kidney fat values rather than to the subcutaneous one. Concerning effects of feed level, growth of FTB lambs fed silage is higher than those fed oat hay. With moderate concentrate supply, FTB lambs' growth is more pronounced on pasture diet than on the feedlot (FL) diet. Furthermore, at similar slaughter weights, carcasses of lambs fed pasture diet have less tail and carcass fat than those from lambs fed FL diet (5% and 18% vs. 9% and 24% for grazing and FL lambs, respectively). No difference in fat colour, fat firmness or cooked meat flavour is observed between carcasses obtained at different slaughtering weight (i.e. from 25 to 35 kg). The tail docking of FTB improved lambs' growth particularly before weaning. Its effect on carcass composition and fat proportion depends on stage of slaughtering and type of fattening diet. For suckled lambs (4 months), the docking resulted in the lower carcass fat weight (and proportion), while for fattened lambs, carcass composition was similar for all types of lambs. Overall, FTB lambs always grow slower than Noire de Thibar lambs. This is particularly pronounced during the fattening phase. Then, for FTB breed, the possibilities to obtain heavy carcasses are at risks of fat accumulation, 22.8% vs. 14.4% for FTB and Noire de Thibar thin-tailed lambs, respectively. However, the main advantage of FTB breed is that adults are well adapted to food scarcity and may produce lambs even under harsh conditions.