An in vitro micro-CT assessment of bioactive restorative materials interfacial adaptation to dentin.

Background: The background of this study was to improve the longevity of a restoration and optimal adaptation of restorative material to the prepared cavity walls is crucial. The study aimed to evaluate the interfacial adaptation of Activa, Micron, and Predicta bulk bioactive restorative materials to coronal dentin using micro-computed tomography (CT) analysis. Materials and Methods: In this in vitro micro-CT assessment study, Class II mesio- and disto-occlusal cavities were prepared on 60 extracted human mandibular molar teeth. After etching and bonding procedures, all the mesial cavities (n = 60) were restored with Tetric N-Ceram and the disto-occlusal cavities with Activa or Micron or Predicta bioactive (n = 20 each) restoratives. Interfacial gap percentages were evaluated under micro-CT before (baseline) and after thermo-mechanical load cycling (TMC). Acquired data were analyzed statistically using one-way analysis of variance, Paired t-test, and Tukey's multiple post hoc procedures, at P < 0.05 level of significance. Results: The interfacial gap percentages were lowest for Predicta bioactive and highest for the Micron group (P < 0.05). The number of gaps increased significantly after TMC in all the groups (P < 0.05). The adaptation of tested materials was inferior to axial wall and pulpal floor, whereas considerably better adaptation was observed on buccal and lingual walls. Conclusion: Predicta bioactive followed by Activa bioactive has shown superior interfacial adaptation, whereas Micron bioactive demonstrated maximum microgaps compared to nanohybrid composite. Artificial aging with TMC has a negative influence on the internal adaptation of all tested materials.

An In-vitro Fracture Strength Assessment of Endodontically Treated Teeth with Different Root-End Materials.

OBJECTIVE: To assess and compare the fracture strength of endodontically treated teeth when the retrograde preparations were restored either with Biodentine or Endosequence BC RRM Fast set putty or Geristore. METHODS: One hundred and twenty human mandibular premolars were used and allocated randomly into five groups (n=24 each). Following conventional root canal treatment, and apical root resection, retrograde cavities of 3 mm were prepared using ultrasonic tips. Group 1 (intact, sound teeth), Group 2 (without rootend filling), Groups 3, 4 and 5were allocated for Biodentine, Endosequence BC RRM putty, and Geristore respectively. Thermo-mechanical cyclic loading (TMC) was performed for one section of samples in each group (n=12 each) following which immediate and after TMC fracture resistance was evaluated using the Instron machine. One-way ANOVA followed by Tukey's multiple post-hoc procedures was used for data analysis. RESULTS: Intact teeth had shown the highest fracture strength values than all other four groups (P<0.05) and resected roots without root-end filling group exhibited the lowest resistance to fracture. Amongst the test groups, Endosequence BC RRM putty displayed improved fracture strength, and Geristore exhibited the least resistance to fracture. Fracture strength values were not statistically different among Endosequence fast set putty and Biodentine group samples immediately and after thermo-mechanical cyclic loading (P=0.5987 and 0.9999 respectively). The fracture strength was notsignificantly different between Geristore and without root-end filling groups (P=0.3530). CONCLUSION: Endodontically treated teeth with Endosequence BC RRM putty or Biodentine root-end filled teeth had shown better fracture resistance compared to Geristore. Retrofilling with Geristore was not able to improve fracture strength of root canal-treated teeth.

Relationship between the Depth of Penetration and Fracture Resistance of Various Sealers: A Comparative Study.

AIM: The aim of this in vitro study was to estimate the penetration depth and fracture resistance of three different sealers and to verify the relationship between the penetration depth and fracture resistance. MATERIALS AND METHODS: Sixty single-rooted teeth were selected and root canal preparation was done. After the instrumentation, the teeth were divided into three groups of 20 each. The groups were then obturated with gutta-percha (GP)-AH Plus sealer, Resilon-Real Seal, and propoint-bioceramic sealers, respectively. Ten teeth from each group were sectioned at three different regions (i.e., coronal, middle, and apical thirds of the root canal) and were viewed under a confocal microscope to determine the penetration depth of the sealer. The remaining samples were subjected to fracture resistance under a universal testing machine and the statistical analysis was done by using one-way ANOVA and post hoc Bonferroni tests. RESULTS: Propoint-bioceramic group showed the highest fracture resistance values followed by GP-AH Plus sealer and Resilon-Real seal groups with no significant difference noticed between them. Depth of penetration was greater for GP-AH Plus sealer, propoint-bioceramic, with no significant difference followed by the Resilon-Real seal group. CONCLUSION: The newer obturating material propoint-bioceramic group showed a greater fracture resistance. No correlation could be established between the depth of sealer penetration and fracture resistance. CLINICAL SIGNIFICANCE: Fracture resistance of tooth obturated with propoint-bioceramic sealer combination is significantly greater than GP-AH Plus and Resilon-Real seal combination, thereby showing propoint-bioceramic as a promising obturating material.

Preemptive screening of DPYD as part of clinical practice: high prevalence of a novel exon 4 deletion in the Finnish population.

Capecitabine is a fluoropyrimidine that is widely used as a cancer drug for the treatment of patients with a variety of cancers. Unfortunately, early onset, severe or life-threatening toxicity is observed in 19-32% of patients treated with capecitabine and 5FU. Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme in the degradation of 5FU and a DPD deficiency has been shown to be a major determinant of severe fluoropyrimidine-associated toxicity. DPD is encoded by the DPYD gene and some of the identified variants have been described to cause DPD deficiency. Preemptive screening for DPYD gene alterations enables the identification of DPD-deficient patients before administering fluoropyrimidines. In this article, we describe the application of upfront DPD screening in Finnish patients, as a part of daily clinical practice, which was based on a comprehensive DPYD gene analysis, measurements of enzyme activity and plasma uracil concentrations. Almost 8% of the patients (13 of 167 patients) presented with pathogenic DPYD variants causing DPD deficiency. The DPD deficiency in these patients was further confirmed via analysis of the DPD activity and plasma uracil levels. Interestingly, we identified a novel intragenic deletion in DPYD which includes exon 4 in four patients (31% of patients carrying a pathogenic variant). The high prevalence of the exon 4 deletion among Finnish patients highlights the importance of full-scale DPYD gene analysis. Based on the literature and our own experience, genotype preemptive screening should always be used to detect DPD-deficient patients before fluoropyrimidine therapy.

Microtensile Bond Strength Evaluation of Composite Resin to Discolored Dentin After Amalgam Removal.

Background To obtain prolonged clinical success with composite restorations, better bonding of resin to the tooth substrate is crucial. Aim The study was aimed to evaluate the microtensile bond strength (µTBS) of bulk-fill composite resin restorations when bonded to a cavity previously restored with amalgam, comparing with that of freshly prepared dentin. Materials and method Mesio-occlusal cavity preparations were done on 80 extracted human mandibular molars with a buccolingual width of 4 mm and a 1.5 mm axial depth by placing the gingival seat 0.5 mm coronal to the cementoenamel junction (CEJ) and were restored with fine-grain amalgam alloy. After thermocycling, the amalgam restorations were removed. Disto-occlusal cavities with similar dimensions of mesial cavities were prepared, and both the proximal surfaces were filled with bulk-fill composite using either etch-and-rinse or self-etch adhesives. Following thermomechanical cyclic loading, all the teeth were sectioned for µTBS testing. Bond strength data expressed in megapascals (MPa) were subjected to statistical analyses using analysis of variance (ANOVA) and Tukey's multiple post-hoc tests. Results The total-etch adhesive exhibited statistically higher bond strength values to both dentin substrates compared to self-etch adhesives (p<0.05). Failure mode analysis reported more of adhesive failures. Conclusion The µTBS of bulk-fill composite resin restorations bonded to a cavity previously restored with amalgam was significantly lower than that of freshly prepared dentin. Total-etch adhesives bond strength was higher than self-etch adhesives to both the substrates tested.

Effect of pretreatment medication on postendodontic pain: A double-blind, placebo-controlled study.

BACKGROUND: Posttreatment endodontic pain has been reported in 25%-40% of all endodontic patients. Effective management of endodontic pain represents a continuing challenge. AIM: To evaluate and compare the efficacy of preoperative single dose of nonsteroidal anti-inflammatory drug, piroxicam (20 mg), with two types of corticosteroid drugs - dexamethasone (4 mg) or deflazacort (30 mg) - for the prevention and control of postendodontic pain. MATERIALS AND METHODS: A total of 132 volunteers selected for nonsurgical root canal therapy were randomly divided into the following four groups (n = 30 each) according to preoperative medication given: Group 1, piroxicam (20 mg); Group 2, dexamethasone (4 mg); Group 3, deflazacort (30 mg); and Group 4, placebo. The preoperative medications were administrated 1 h before the start of standard endodontic treatment. Patients were instructed to complete a pain diary using Visual Analog Scale preoperatively and at 6-, 12-, 24-, 48-, and 72-h intervals after root canal instrumentation. STATISTICAL ANALYSIS: The correlation between preoperative endodontic pain to postoperative pain and pair-wise comparison of four groups was evaluated by Kruskal-Wallis analysis of variance test followed by Mann-Whitney U-test. RESULTS: Compared to the placebo group, piroxicam, dexamethasone, and deflazacort resulted in a statistically significant reduction in postendodontic pain at 6, 12, and 24 h (P < 0.05). CONCLUSION: Preoperative single oral dose of piroxicam or dexamethasone or deflazacort is equally effective in controlling postendodontic pain.

Micro-computed tomographic evaluation of dentinal defects after root canal preparation with hyflex edm and vortex blue rotary systems.

BACKGROUND: To evaluate the incidence of dentinal microcracks formation after root canal shaping procedures with HyFlex EDM and Vortex Blue rotary systems comparing with that of hand instrumentation using micro-computed tomography. MATERIAL AND METHODS: Mandibular first molar teeth (n=60) having 50 to 200mesial root curvature were scanned using high resolution micro-CT imaging before root canal preparation to identify the presence of dentinal defects. Post-instrumentation cross-sectional images were taken and increased number and type of root defects were assessed and recorded. The data was statistically analysed by using chi-square test and McNemar's test at a significance level of 5%. RESULTS: HyFlex EDM has shown greater increase in post instrumentation dentinal defects. Both rotary systems exhibited increased dentinal defects formation at coronal, middle regions which is statistically significant (P=0.042) when compared to apical region. CONCLUSIONS: HyFlex EDM has shown greater increase in post instrumentation dentinal defects followed by Vortex Blue rotary system and hand instrumentation resulted minimal defects. Key words:Craze lines, dentinal microcracks, micro-computed tomography, nickel-titanium files, root canal preparation.

Microtensile bond strength of bulk-fill restorative composites to dentin.

BACKGROUND: To facilitate the easier placement of direct resin composite in deeper cavities, bulk fill composites have been introduced. The Mechanical stability of fillings in stress bearing areas restored with bulk-fill resin composites is still open to question, since long term clinical studies are not available so far. Thus, the objective of the study was to evaluate and compare the microtensile bond strength of three bulk-fill restorative composites with a nanohybrid composite. MATERIAL AND METHODS: Class I cavities were prepared on sixty extracted mandibular molars. Teeth were divided into 4 groups (n= 15 each) and in group I, the prepared cavities were restored with nanohybrid (Filtek Z250 XT) restorative composite in an incremental manner. In group II, III and IV, the bulk-fill composites (Filtek, Tetric EvoCeram, X-tra fil bulk-fill restoratives) were placed as a 4 mm single increment and light cured. The restored teeth were subjected to thermocycling and bond strength testing was done using instron testing machine. The mode of failure was assessed by scanning electron microscope (SEM). The bond strength values obtained in megapascals (MPa) were subjected to statistical analysis, using SPSS/PC version 20 software.One-way ANOVA was used for groupwise comparison of the bond strength. Tukey's Post Hoc test was used for pairwise comparisons among the groups. RESULTS: The highest mean bond strength was achieved with Filtek bulk-fill restorative showing statistically significant difference with Tetric EvoCeram bulk-fill (p< 0.003) and X-tra fil bulk-fill (p<0.001) composites. Adhesive failures are mostly observed with X-tra fil bulk fill composites, whereas mixed failures are more common with other bulk fill composites. CONCLUSIONS: Bulk-fill composites exhibited adequate bond strength to dentin and can be considered as restorative material of choice in posterior stress bearing areas. Key words:Bond strength, Bulk-fill restoratives, Configuration factor, Polymerization shrinkage.